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Showing AURKB — AURB is a alias.

AURKB

Aurora kinase B · UniProt Q96GD4

Length
344 aa
Mass
39.3 kDa
Annotated
2026-06-09
100 papers in source corpus 48 papers cited in narrative 47 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

AURKB (Aurora B kinase, the IPL1/AIR-2/AIM-1 ortholog) is the catalytic engine of high-fidelity chromosome segregation, functioning as the kinase subunit of the chromosomal passenger complex (CPC) together with INCENP/Sli15, Survivin/Bir1, and Borealin/Nbl1, which together control CPC localization, stability, and activity (PMID:8007975, PMID:7874197, PMID:10385519, PMID:19158380). Its kinase activity is stimulated by direct association with INCENP/Sli15, which also targets it to the mitotic spindle (PMID:10385519, PMID:11724818). AURKB enforces chromosome bi-orientation by phosphorylating outer-kinetochore and microtubule-binding substrates — Ndc10, the Dam1/DASH complex, and Ndc80 — to weaken improper kinetochore-microtubule attachments and promote their turnover until tension is established, at which point substrate dephosphorylation by the opposing PP1/Glc7 phosphatase stabilizes correct attachments (PMID:10072382, PMID:11724818, PMID:19923271, PMID:19822728, PMID:28928489, PMID:16537909). By converting tension defects into unattached-kinetochore signals it activates the spindle assembly checkpoint specifically in response to loss of tension (PMID:11731476, PMID:16327780). AURKB also phosphorylates histone H3 on serine 10 during mitosis (PMID:10975519, PMID:19704020), and drives cytokinesis, spindle midzone organization, and spindle disassembly through phosphorylation of midzone regulators such as Ase1 and through CPC relocalization to the central spindle (PMID:9809983, PMID:9852156, PMID:12566427, PMID:17765685). Its spatiotemporal control is layered: Cdk1 phosphorylates AURKB and INCENP/Sli15 to restrain premature spindle binding until anaphase (PMID:21727193, PMID:22521784), haspin-generated H3T3ph and Shugoshin recruit it to centromeres (PMID:35694956, PMID:24945276), USP29-mediated deubiquitination stabilizes it (PMID:38233848), and VRK1 cross-inhibition modulates its H3 phosphorylation (PMID:29340707). In specialized and disease contexts AURKB remodels meiotic kinetochores and protects meiotic cohesion (PMID:17371833, PMID:23371552, PMID:26157162), resets Oct4-driven pluripotency transcription in stem cells (PMID:26880562), and acts in interphase as a transcriptional regulator by depositing promoter H3S10ph at genes including CCND1, CCNE1, and TERT (PMID:31982864, PMID:38713155, PMID:37079315).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1994 High

    Established that a kinase activity opposed by a phosphatase governs chromosome segregation fidelity, defining the foundational kinase-phosphatase balance later shown to act on Aurora B substrates.

    Evidence Genetic epistasis and dosage suppressor screens in budding yeast linking IPL1 and GLC7/PP1

    PMID:7874197 PMID:8007975

    Open questions at the time
    • Did not identify direct substrates of the kinase
    • Mechanism of opposition (substrate vs kinase regulation) unresolved
  2. 1998 High

    Showed the kinase controls cytokinesis distinct from nuclear division, separating its cleavage-furrow role from chromosome functions.

    Evidence Dominant-negative AIM-1 expression and RNAi of AIR-2 with live imaging of cytokinesis in rat cells and C. elegans

    PMID:9809983 PMID:9852156

    Open questions at the time
    • Cytokinesis substrates not identified
    • Mechanism of midbody relocalization unknown
  3. 1999 High

    Connected the kinase mechanistically to kinetochores by identifying a direct kinetochore substrate and a stable activating partner.

    Evidence In vitro kinase assay on Ndc10p, kinetochore-microtubule binding from mutant extracts, and Co-IP/epistasis identifying Sli15/INCENP

    PMID:10072382 PMID:10385519

    Open questions at the time
    • Did not establish how phosphorylation alters attachment
    • Full CPC composition not yet defined
  4. 2000 High

    Identified histone H3 serine 10 as a conserved mitotic substrate balanced by the opposing phosphatase, generalizing the kinase-phosphatase axis to chromatin.

    Evidence Genetic analysis and phospho-H3 staining in S. cerevisiae and C. elegans

    PMID:10975519

    Open questions at the time
    • Functional consequence of H3S10ph for segregation not resolved
    • Direct vs indirect phosphorylation in vivo not separated here
  5. 2001 High

    Defined the kinase as the agent of bi-orientation and tension-sensing, distinguishing tension defects from attachment defects in checkpoint signaling.

    Evidence Live imaging of kinetochore attachment turnover, checkpoint arrest assays, and in vitro kinase/microtubule-binding assays on Sli15 and Dam1

    PMID:11724818 PMID:11731476 PMID:11853667

    Open questions at the time
    • Did not show how tension is read mechanically
    • Relative contribution of individual substrates unquantified
  6. 2005 High

    Demonstrated directly that the kinase generates the checkpoint signal by converting tensionless attachments into unattached kinetochores, and uncovered methylation antagonism as a substrate-level control.

    Evidence Analog-sensitive kinase allele with checkpoint and attachment assays; in vitro Set1 methylation inhibiting Dam1 phosphorylation

    PMID:16143104 PMID:16327780

    Open questions at the time
    • Physical basis of detachment after phosphorylation not fully defined
    • Generality of methyl-phospho switch to other substrates unknown
  7. 2006 High

    Resolved that the opposing phosphatase acts on the kinase's substrates rather than on the kinase itself, clarifying the directionality of the regulatory balance.

    Evidence Phosphorylation assays, localization, and dosage suppressor genetics on Glc7/PP1 regulatory subunits Gip3/Gip4 and Dam1

    PMID:16537909

    Open questions at the time
    • Spatial coordination of phosphatase targeting incompletely mapped
  8. 2007 High

    Extended kinase function to meiotic cohesion protection and centrosome-driven spindle assembly through new substrates and recruitment factors.

    Evidence Genetic/localization analysis of Sgo1-dependent recruitment and PP2A-Rts1 maintenance; phospho-site mutagenesis of the midzone protein Ase1

    PMID:17371833 PMID:17765685

    Open questions at the time
    • Direct phosphorylation of cohesion machinery not shown
    • Ase1 role outside Cin8-deficient context unclear
  9. 2009 High

    Built the mechanistic detail of substrate regulation by tension and added core CPC subunit Nbl1, while extending the kinase to mitotic chromatin RNA control and meiotic SC disassembly.

    Evidence Phospho-specific antibodies tracking Dam1/Ndc80 phosphorylation vs tension, Co-purification of Nbl1, RNAi/imaging of XIST retention, and meiotic SC disassembly genetics

    PMID:19158380 PMID:19704020 PMID:19759266 PMID:19822728 PMID:19923271

    Open questions at the time
    • Why single-substrate phospho-mutants are insufficient remains open
    • Mechanism linking H3 phosphorylation to RNA retention not defined
  10. 2012 High

    Established Cdk1 as a direct upstream regulator that times CPC spindle binding, integrating Aurora B into the mitotic kinase network.

    Evidence In vitro kinase assay, phospho-site mutagenesis, and localization of Cdk1-phosphorylated Sli15/Ipl1 controlling Bim1 association

    PMID:21727193 PMID:22521784

    Open questions at the time
    • Coordination with phosphatase counteraction at anaphase not fully resolved
  11. 2015 High

    Showed the kinase remodels kinetochores and orchestrates attachment release for meiosis, defining a meiosis-specific structural role.

    Evidence Live imaging with chemical-genetic kinase inhibition and quantitative mass spectrometry showing Ipl1-dependent Ndc80 shedding in meiosis I

    PMID:23371552 PMID:26157162

    Open questions at the time
    • Substrate driving Ndc80 shedding not pinpointed
    • Sequential kinase handoff mechanism with Mps1 incompletely mapped
  12. 2018 High

    Expanded the regulatory network upstream and laterally — VRK1 cross-inhibition, haspin-dependent centromere recruitment, and inter-Aurora kinase regulation in meiosis.

    Evidence Co-IP and reciprocal in vitro kinase inhibition (VRK1); haspin inhibitor/knockout localization in spermatocytes; double-knockout mouse oocyte genetics (AURKB/AURKC)

    PMID:29340707 PMID:30415701 PMID:35694956

    Open questions at the time
    • Quantitative balance among recruitment inputs unresolved
    • Tissue specificity of inter-kinase regulation not generalized
  13. 2019 Medium

    Defined an interphase transcriptional function in which promoter H3S10ph activates growth genes, and positioned AURKB as a context-dependent therapeutic target.

    Evidence ChIP of H3S10ph at CCND1, EHMT phosphorylation in B-ALL, and inhibitor studies in NSCLC, TNBC, and KSHV cleavage models

    PMID:30733284 PMID:30917319 PMID:31000705 PMID:31982864 PMID:36627281

    Open questions at the time
    • Direct vs recruited H3S10ph at specific promoters not always separated
    • Generality of transcriptional roles beyond surveyed cancers unknown
  14. 2022 Medium

    Identified post-translational stabilization controlling AURKB abundance, linking deubiquitination to oncogenic signaling.

    Evidence Co-IP, ubiquitination assays, ChIP, and Usp29 knockout mice defining a FUBP1-USP29-AURKB axis

    PMID:38233848

    Open questions at the time
    • Whether stabilization affects mitotic vs transcriptional pools unclear
  15. 2024 Medium

    Revealed kinase-independent and noncanonical activities, including RNA-binding-protein regulation and additional promoter H3S10ph targets.

    Evidence Co-IP/RNA-IP and mRNA stability assays for HNRNPM/PSAT1 (kinase-independent), DHX9 and MAD2L2 interaction studies, and ChIP at the CCNE1 promoter

    PMID:38515112 PMID:38713155 PMID:38874176 PMID:40784984

    Open questions at the time
    • Structural basis of kinase-independent RNA-protein interference unknown
    • DHX9 interaction rests on Co-IP without reciprocal validation

Open questions

Synthesis pass · forward-looking unresolved questions
  • How AURKB partitions between its canonical CPC mitotic role and its emerging kinase-independent and interphase transcriptional functions, and how these are coordinately regulated in normal versus malignant cells, remains unresolved.
  • No unified model integrating mitotic and transcriptional pools
  • Substrate hierarchy across contexts not quantified
  • Structural determinants of kinase-independent functions undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 7 GO:0042393 histone binding 5 GO:0008092 cytoskeletal protein binding 4 GO:0016740 transferase activity 4 GO:0140110 transcription regulator activity 4 GO:0003723 RNA binding 1
Localization
GO:0005856 cytoskeleton 5 GO:0005694 chromosome 4 GO:0005634 nucleus 3 GO:0000228 nuclear chromosome 2 GO:0005815 microtubule organizing center 2
Pathway
R-HSA-1643685 Disease 7 R-HSA-1474165 Reproduction 6 R-HSA-1640170 Cell Cycle 5 R-HSA-74160 Gene expression (Transcription) 4 R-HSA-4839726 Chromatin organization 3
Partners
BIRC5BOREALIN/NBL1DAM1INCENPMAD2L2NDC80USP29VRK1
Complex memberships
Chromosomal passenger complex (CPC)

Evidence

Reading pass · 47 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1994 IPL1 encodes a protein kinase required for high-fidelity chromosome segregation in budding yeast. Type 1 protein phosphatase (PP1/GLC7) acts in opposition to Ipl1 kinase to ensure proper chromosome segregation: overexpression of GLC7 causes chromosome missegregation in wild-type cells, and glc7-1 mutation can partially suppress ipl1-1, establishing these two enzymes as opposing regulators of chromosome segregation. Genetic epistasis, dosage suppressor screen, conditional mutant analysis Molecular and cellular biology High 7874197 8007975
1999 Ipl1p (Aurora B ortholog) regulates microtubule binding to kinetochores; kinetochores assembled from ipl1 mutant extracts show altered microtubule binding, and Ipl1p phosphorylates the kinetochore component Ndc10p in vitro. Ipl1p localizes to the mitotic spindle with cell-cycle-regulated levels. In vitro kinase assay (Ndc10p phosphorylation), kinetochore-microtubule binding assay from ipl1 mutant extracts, immunofluorescence localization Genes & development High 10072382
2000 Ipl1/Aurora kinase and its genetically interacting phosphatase Glc7/PP1 are responsible for the balance of histone H3 serine 10 phosphorylation during mitosis in S. cerevisiae and C. elegans. Both enzymes are required for H3 phosphorylation and chromosome segregation. Genetic analysis in S. cerevisiae and C. elegans, phospho-H3 staining, epistasis Cell High 10975519
1998 AIM-1 (rat Aurora B ortholog) controls entry into cytokinesis during M phase; kinase-negative AIM-1 inhibits cleavage furrow formation without affecting nuclear division. Overexpression of wild-type AIM-1 produces multinuclearity and increased ploidy. Dominant-negative kinase mutant expression, live cell imaging of cytokinesis, flow cytometry for ploidy Cancer research Medium 9809983
1998 AIR-2 (C. elegans Aurora B ortholog) is required for polar body extrusion and cytokinesis; RNAi-mediated depletion of AIR-2 results in failed cytokinesis with transient cleavage furrow initiation followed by regression, and mislocalization of midbody microtubule components. AIR-2 localizes to chromosomes during meiosis, moves to midbody microtubules at anaphase, and persists at the cytokinesis remnant. RNAi knockdown in C. elegans, immunofluorescence localization, live imaging The Journal of cell biology High 9852156
1999 Sli15 (INCENP ortholog) associates directly with the Ipl1 protein kinase in vivo; both proteins co-localize to the mitotic spindle. sli15 mutant phenotype is very similar to ipl1 mutants and is partially suppressed by reduced PP1 activity, establishing Sli15 as a key functional partner of Ipl1 in chromosome segregation. Co-immunoprecipitation, genetic epistasis, immunofluorescence The Journal of cell biology High 10385519
2001 Ipl1p (Aurora B) is required to maintain spindle checkpoint arrest induced by lack of tension at kinetochores but is not required for arrest induced by spindle depolymerization. Ipl1p localizes at or near kinetochores during mitosis, distinguishing two mechanistically distinct spindle checkpoint signals. Genetic analysis, checkpoint arrest assays, immunofluorescence localization Genes & development High 11731476
2001 The Ipl1-Sli15 complex promotes chromosome bi-orientation by altering kinetochore-spindle pole connections. In ipl1 mutants, kinetochores remain attached to old spindle pole bodies and fail to turn over attachments, suggesting Ipl1-Sli15 facilitates bi-orientation by promoting turnover of kinetochore-microtubule connections until tension is established. Live-cell imaging in spindle pole body duplication assay, genetic analysis Cell High 11853667
2001 Sli15 stimulates the in vitro kinase activity of Ipl1 and facilitates Ipl1's association with the mitotic spindle. Both Ipl1 and Sli15 bind Dam1 (a microtubule-binding kinetochore protein) directly, and Ipl1 phosphorylates both Sli15 and Dam1 in vitro with reduced in vivo phosphorylation in ipl1 mutants. Sli15 and Ipl1 also bind microtubules directly in vitro and are associated with yeast centromeric DNA in vivo. In vitro kinase assay, Co-immunoprecipitation, microtubule binding assay, chromatin immunoprecipitation The Journal of cell biology High 11724818
2003 Ipl1p has a role in mitotic spindle disassembly separable from its chromosome segregation functions. Ipl1-GFP transfers from kinetochores to the spindle after metaphase and accumulates at the spindle midzone late in anaphase; Ipl1p kinase activity increases at anaphase, and ipl1 mutants can stabilize fragile spindles. Time-lapse microscopy, GFP-tagging, kinase activity assays The Journal of cell biology Medium 12566427
2005 Ipl1/Aurora activates the spindle checkpoint in response to tension defects by creating unattached kinetochores. When Ipl1 function was impaired in kinetochore mutants that appear to have unattached kinetochores, microtubule attachments were restored and the checkpoint was turned off, demonstrating that Ipl1 converts tension defects into unattached kinetochore signals. Chemical-genetic (analog-sensitive allele), spindle checkpoint assays, kinetochore attachment assays Nature cell biology High 16327780
2005 The Set1 methyltransferase modulates the Ipl1/Glc7 balance. Set1 methylates conserved lysines in the kinetochore protein Dam1, and Dam1 methylation inhibits Ipl1-mediated phosphorylation of flanking serines, demonstrating antagonism between lysine methylation and serine phosphorylation as a mechanism controlling Ipl1 substrate activity. Genetic epistasis, in vitro kinase assays, biochemical methylation assays Cell High 16143104
2006 Glc7/PP1 ensures accurate chromosome segregation by dephosphorylating Ipl1 targets (particularly Dam1) rather than by regulating Ipl1 kinase levels or activity. Regulatory subunits Gip3 and Gip4 suppress ipl1-321 by redistributing Glc7 away from Ipl1 targets, restoring the balance of Dam1 phosphorylation. Phosphorylation assays, localization studies, dosage suppressor screen, genetic epistasis Molecular and cellular biology High 16537909
2007 Aurora B kinase (Ipl1) in yeast is essential for protection of meiotic centromeric cohesion. Sgo1 recruits Ipl1 to centromeric regions, and in the absence of Ipl1, the PP2A regulatory subunit Rts1 cannot be maintained at centromeres after anaphase I onset, leading to loss of cohesion protection. Genetic analysis, localization studies, immunofluorescence The Journal of cell biology Medium 17371833
2007 Ipl1/Aurora kinase is required for centrosome-mediated spindle assembly in the absence of BimC motor Cin8. Ipl1 regulates Ase1 (spindle midzone protein) by phosphorylating it; an Ase1 mutant lacking Ipl1 consensus phosphorylation sites cannot assemble spindles in the absence of Cin8, and Ase1 phosphorylation and localization are altered in ipl1 mutants. Genetic epistasis, phosphorylation site mutagenesis, spindle assembly assays, fluorescence microscopy Developmental cell High 17765685
2009 AURKB phosphorylates H3 at prophase, and RNAi knockdown of AURKB causes mitotic retention of XIST RNA on the inactive X chromosome, demonstrating that AURKB-mediated H3 phosphorylation regulates RNA binding to heterochromatin during mitosis. H3S10 phosphorylation (but not H3S28ph) is excluded from the inactive X and potentially linked to ubiquitination. RNAi knockdown, pharmacological inhibition, immunofluorescence, RNA-FISH The Journal of cell biology Medium 19704020
2009 Ipl1-dependent phosphorylation of the kinetochore protein Dam1 is maximal during S phase and minimal during metaphase; when tension at kinetochores is reduced by failure to establish sister chromatid cohesion, Dam1 phosphorylation persists in metaphase, indicating that tension leads to dephosphorylation of Ipl1 substrates, stabilizing bi-orientation. Phospho-specific antibodies, genetic manipulation of tension, cell cycle synchronization Journal of cell science High 19923271
2009 Phosphorylation of the Ndc80 kinetochore protein by Ipl1/Aurora B reduces its microtubule binding activity in vitro, and kinetochore-bound Ndc80 is phosphorylated on Ipl1 sites in vivo; however, this phosphorylation is not essential alone, indicating additional Ipl1 targets contribute to segregation and checkpoint signaling. In vitro microtubule binding assay with phosphorylation, in vivo phosphorylation analysis, genetic analysis Genetics Medium 19822728
2009 Nbl1p is a new core component of the chromosomal passenger complex (CPC) in budding yeast, related to Borealin/Dasra. Nbl1p colocalizes and co-purifies with the CPC (Ipl1/Aurora B, Sli15/INCENP, Bir1/Survivin), is essential for CPC localization, stability, integrity, and function. Structure modeling revealed structural conservation of the CPC architecture from Fungi to Animalia. Co-purification, co-localization, genetic analysis, comparative structural modeling Molecular biology of the cell High 19158380
2009 Ipl1/Aurora B coordinates synaptonemal complex (SC) disassembly with cell cycle progression in budding yeast meiosis. Ipl1 mutants fail to dissociate the central element Zip1 and its binding partner Smt3/SUMO from chromosomes in a timely fashion, and SC disassembly delay occurs even in cdc5 or NDT80-regulated backgrounds. Genetic analysis, immunofluorescence, meiotic progression assays Genes & development Medium 19759266
2011 Ipl1/Aurora B-dependent phosphorylation of Sli15/INCENP modulates microtubule dynamics by preventing CPC binding to the preanaphase spindle and to the central spindle until late anaphase. Decreased Ipl1-dependent Sli15 phosphorylation drives direct CPC-microtubule binding, revealing how CPC influences microtubule dynamics. Cdk1 and Ipl1/Aurora cooperatively modulate microtubule dynamics. Phosphorylation site mutagenesis, live-cell imaging, microtubule dynamics assays The Journal of cell biology High 21727193
2011 Aurora kinase Ipl1 is necessary for maintenance of tight association (cohesion) between duplicated spindle pole bodies (SPBs) during meiosis. Loss of Ipl1 leads to premature SPB separation, overduplication, and multipolar spindles. The Polo-like kinase Cdc5 interacts antagonistically with Ipl1 at the meiotic SPB. Genetic analysis, fluorescence microscopy, SPB localization assays Journal of cell science Medium 21878496
2012 Cdk1 directly phosphorylates Ipl1/Aurora B on two serine residues in the N-terminal domain, suppressing its association with the microtubule plus-end tracking protein Bim1 until anaphase onset. Failure to phosphorylate Ipl1 leads to premature targeting to the metaphase spindle and constitutive Bim1 phosphorylation, and the non-phosphorylatable Ipl1-Sli15 complex causes severe growth defects. In vitro kinase assay, phosphorylation site mutagenesis, localization studies, genetic analysis Current biology : CB High 22521784
2013 Ipl1/Aurora B releases kinetochore-microtubule associations after meiotic entry, liberating chromosomes for homologous pairing in meiosis I. Ipl1 also releases improper kMT connections established early in meiosis, while Mps1 triggers formation of new force-generating attachments, establishing a sequential kinase mechanism for correct chromosome orientation. Live-cell imaging, chemical genetic inhibition of Ipl1 and Mps1, fluorescence microscopy Science High 23371552
2014 Ipl1/Aurora B-dependent phosphorylation of Sli15 on microtubule-binding domain sites inhibits Sli15-microtubule interaction in vitro; mimicking constitutive phosphorylation delocalizes the CPC in metaphase, while blocking phosphorylation drives excessive spindle localization. These Ipl1-phosphorylation events also regulate the tension checkpoint mechanism. Phosphorylation site mutagenesis, in vitro microtubule binding assay, in vivo localization studies, checkpoint assay PloS one High 24558497
2014 Shugoshin (Sgo1) in budding yeast maintains Aurora B/Ipl1 localization on kinetochores during metaphase and also recruits condensin to centromeric chromatin via PP2A-Rts1, demonstrating a dual function of shugoshin in promoting biorientation. Genetic analysis, localization studies, immunofluorescence PLoS genetics Medium 24945276
2015 Ipl1/Aurora B is necessary for kinetochore restructuring in meiosis I. Upon meiotic entry, the Ndc80 outer kinetochore complex (but not other subcomplexes) is shed from kinetochores in an Ipl1-dependent manner, promoting assembly of a meiosis-specific kinetochore that confers correct segregation patterns. Quantitative mass spectrometry of kinetochore components, imaging, genetic analysis Molecular biology of the cell High 26157162
2016 Aurora B (Aurkb) phosphorylates Oct4 at serine 229 during G2/M, leading to dissociation of Oct4 from chromatin in embryonic stem cells (ESCs). PP1 then binds Oct4 and dephosphorylates S229 during M/G1 transition, resetting Oct4-driven transcription for pluripotency and cell cycle genes. Phospho-mimetic and PP1-binding-deficient Oct4 mutations alter the cell cycle and cause loss of pluripotency. In vitro kinase assay, phosphorylation site mutagenesis, chromatin binding assays, ESC functional assays eLife High 26880562
2017 Rad52 is a substrate of Ipl1/Aurora B kinase in yeast and humans (confirmed by in vitro kinase assay). Ipl1-dependent phosphorylation of Rad52 facilitates kinetochore accumulation of Mps1, linking Aurora B activity to spindle assembly checkpoint regulation via Rad52. In vitro kinase assay, kinetochore localization assays, genetic epistasis Proceedings of the National Academy of Sciences of the United States of America Medium 29078282
2017 Phosphorylation of Dam1 by Ipl1/Aurora B kinase at three key serine residues in vivo promotes chromosome bipolar attachment. Phospho-deficient dam1-3A mutants show stabilized kinetochore-microtubule attachment, delay establishment of bipolar attachment after nocodazole washout, and exhibit dramatic chromosome missegregation. Phospho-deficient mutant analysis in vivo, chromosome segregation assays, spindle checkpoint assays Scientific reports Medium 28928489
2018 AURKB (Aurora B) phosphorylates survivin, and PLK1 also phosphorylates survivin at different sites to affect cell proliferation. AURKB and PLK1 are required for growth of African American but not European American triple-negative breast cancer (TNBC) xenografts, establishing a context-specific requirement for this phosphorylation axis. Kinase assays, xenograft tumor models, pharmacological inhibition (barasertib, volasertib), siRNA knockdown Cell death & disease Medium 36627281
2018 VRK1 and AURKB form a stable protein complex; each kinase inhibits the kinase activity of the other and inhibits their respective histone H3 phosphorylations (Thr3 by VRK1, Ser10 by AURKB). Depletion of VRK1 downregulates survivin (BIRC5), preventing AURKB recruitment and localization to centromeres. Co-immunoprecipitation, in vitro kinase inhibition assays, RNAi knockdown, immunofluorescence Cellular and molecular life sciences : CMLS Medium 29340707
2018 In mouse oocyte meiosis, AURKC is the predominant CPC kinase. In the absence of AURKC, AURKA localizes to chromosomes in a CPC-dependent manner, suggesting AURKC prevents AURKA from competing for CPC binding. AURKB negatively regulates AURKC to prevent aneuploidy, revealing inter-kinase regulation critical for meiosis. Single and double knockout mouse models, immunofluorescence localization, oocyte functional assays Current biology : CB High 30415701
2019 AURKB phosphorylates histone H3 at serine 10 (H3S10ph), and this activity activates CCND1 expression through H3S10ph at the CCND1 gene promoter in gastric cancer cells. AZD1152 (AURKB inhibitor) suppresses CCND1 expression and inhibits cell proliferation in vitro and in vivo. Chromatin immunoprecipitation (ChIP) at CCND1 promoter, AURKB knockdown/inhibitor treatment, proliferation assays, xenograft models Aging Medium 31982864
2019 AURKB restrains glucocorticoid (GC) signaling in B-ALL by phosphorylating the histone methyltransferases EHMT1/EHMT2, which are required for GC-induced cell death gene expression. AURKB inhibition enhances GC-induced expression of cell death genes and potentiates GC cytotoxicity in relapsed B-ALL cells. Genome-wide shRNA screen, AURKB inhibitor treatment, gene expression analysis, functional cell death assays Proceedings of the National Academy of Sciences of the United States of America Medium 30733284
2019 In NSCLC cells with acquired resistance to EGFR TKIs, AURKB is activated (measured as increased phospho-histone H3), and AURKB inhibition reduces pH3 levels, triggering G1/S arrest and polyploidy followed by cell death or senescence depending on mutation status. AURKB inhibitor treatment (barasertib, S49076), phospho-H3 measurement, flow cytometry, cell death assays Nature communications Medium 31000705
2019 In KSHV-infected tumor cells, the viral latent antigen LANA cleaves AURKB at Asp76 in a serine protease-dependent manner, generating an N'-AURKB isoform that relocalizes to the spindle pole and promotes metaphase-to-telophase transition, enhancing colony formation and malignant growth. Mass spectrometry identification of cleavage site, mutagenesis, localization studies (immunofluorescence), in vitro/in vivo tumor growth assays Cell reports Medium 30917319
2019 COMA complex (Ctf19/Ame1/Okp1/Mcm21) recruits the Sli15/Ipl1 (INCENP/Aurora B) CPC to the inner kinetochore in budding yeast. The Ctf19 C-terminus interacts with the CPC in vitro, and tethering Sli15 to Ame1/Okp1 rescues lethality from Ctf19 depletion in a Sli15 centromere-targeting deficient mutant. Ame1/Okp1 selectively binds Cse4/CENP-A nucleosomes through the Cse4 N-terminus. Crosslink-guided in vitro reconstitution, Co-IP, genetic rescue experiments eLife High 31112132
2020 CCAT2 lncRNA interacts directly with and stabilizes BOP1, and BOP1 overexpression promotes chromosomal instability by increasing the active form of Aurora kinase B (AURKB), which regulates chromosomal segregation. MS2 pull-down, RNA immunoprecipitation, mass spectrometry, CIN assays Gastroenterology Medium 32805281
2021 BRAF(V600E) induces mitotic arrest in human melanocytes through microRNA-mediated suppression of AURKB. MIR211-5p and MIR328-3p target AURKB mRNA and their overexpression induces mitotic failure, genome duplication, and proliferation arrest. AURKB expression rescues arrested nevus cells from this arrest. miRNA overexpression, luciferase reporter assay for AURKB targeting, AURKB rescue experiment, flow cytometry for ploidy eLife Medium 34812139
2022 Haspin kinase activity is required for recruitment of Aurora B (AURKB) and kinesin MCAK to meiotic centromeres during male meiosis in mice. Haspin inhibition or genetic ablation reduces H3T3 phosphorylation, impairs AURKB centromere localization, and causes chromosome congression defects. Chemical inhibition (LDN-192960), Haspin-/- mouse model, immunofluorescence of spermatocytes Journal of cell science Medium 35694956
2022 USP29 deubiquitinase stabilizes AURKB by suppressing K48-linked polyubiquitination. FUBP1 transcription factor directly activates USP29 gene transcription, constituting a FUBP1-USP29-AURKB regulatory axis promoting gastric cancer. Systemic knockout of Usp29 in mice reduces AURKB levels in forestomach tissues. Co-immunoprecipitation, ubiquitination assay, ChIP, luciferase reporter assay, USP29 knockout mice, mass spectrometry Cancer cell international Medium 38233848
2023 AURKB inhibition (hesperadin) in uveal melanoma reduces H3S10 phosphorylation at the TERT (telomerase reverse transcriptase) promoter, leading to H3K9 methylation and chromatin condensation that silences TERT transcription, demonstrating an epigenetic mechanism by which AURKB controls telomerase expression. Chromatin immunoprecipitation (ChIP), RNA-seq, AURKB inhibitor treatment, in vitro/in vivo tumor models Investigative ophthalmology & visual science Medium 37079315
2024 AURKB interacts with and modulates the expression of MAD2L2 in bladder cancer cells. AURKB activates MAD2L2 expression to downregulate the p53 DNA damage response pathway, promoting cancer cell proliferation and cell cycle progression. MAD2L2 overexpression rescues AURKB knockdown phenotypes in vitro and in vivo. Co-immunoprecipitation, Western blot, CRISPR knockdown, xenograft rescue experiments Journal of translational medicine Medium 38515112
2024 AURKB activates CCNE1 (cyclin E1) expression by phosphorylating histone H3 at serine 10 (H3S10ph) at the CCNE1 promoter in colorectal cancer cells, promoting cell proliferation and tumor growth. Chromatin immunoprecipitation (ChIP) at CCNE1 promoter, AURKB knockdown/inhibitor (AZD1152), xenograft models Aging Medium 38713155
2024 AURKB interacts with and phosphorylates DHX9 (DExH-Box helicase 9), targeting its expression in hepatocellular carcinoma cells, and this AURKB-DHX9 interaction promotes HCC progression through the PI3K/AKT/mTOR pathway. Co-immunoprecipitation, Western blot, functional knockdown and rescue experiments Molecular carcinogenesis Low 38874176
2025 AURKB exerts a kinase-independent oncogenic function in colorectal cancer by binding HNRNPM and interfering with its interaction with PSAT1 mRNA, thereby suppressing HNRNPM-mediated mRNA degradation and increasing PSAT1 protein levels. AURKB transcription in CRC is driven by H3K18 lactylation at its promoter. Mass spectrometry, Co-IP, proximity ligation assay, RNA immunoprecipitation, mRNA stability assay, ChIP-qPCR Journal of experimental & clinical cancer research : CR Medium 40784984

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes. Cell 725 10975519
2002 Evidence that the Ipl1-Sli15 (Aurora kinase-INCENP) complex promotes chromosome bi-orientation by altering kinetochore-spindle pole connections. Cell 603 11853667
1999 The conserved protein kinase Ipl1 regulates microtubule binding to kinetochores in budding yeast. Genes & development 354 10072382
2001 The budding yeast protein kinase Ipl1/Aurora allows the absence of tension to activate the spindle checkpoint. Genes & development 346 11731476
1998 Multinuclearity and increased ploidy caused by overexpression of the aurora- and Ipl1-like midbody-associated protein mitotic kinase in human cancer cells. Cancer research 290 9809983
1998 AIR-2: An Aurora/Ipl1-related protein kinase associated with chromosomes and midbody microtubules is required for polar body extrusion and cytokinesis in Caenorhabditis elegans embryos. The Journal of cell biology 273 9852156
2005 The Ipl1-Aurora protein kinase activates the spindle checkpoint by creating unattached kinetochores. Nature cell biology 248 16327780
1994 Type 1 protein phosphatase acts in opposition to IpL1 protein kinase in regulating yeast chromosome segregation. Molecular and cellular biology 237 8007975
1997 Cell cycle-dependent expression and spindle pole localization of a novel human protein kinase, Aik, related to Aurora of Drosophila and yeast Ipl1. The Journal of biological chemistry 236 9153231
1999 Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3. The Journal of biological chemistry 213 10066797
1992 The G-protein-coupled receptor kinases beta ARK1 and beta ARK2 are widely distributed at synapses in rat brain. The Journal of neuroscience : the official journal of the Society for Neuroscience 177 1403099
2001 Functional cooperation of Dam1, Ipl1, and the inner centromere protein (INCENP)-related protein Sli15 during chromosome segregation. The Journal of cell biology 144 11724818
2003 The budding yeast Ipl1/Aurora protein kinase regulates mitotic spindle disassembly. The Journal of cell biology 137 12566427
2005 Overexpression of aurora B kinase (AURKB) in primary non-small cell lung carcinoma is frequent, generally driven from one allele, and correlates with the level of genetic instability. British journal of cancer 126 16222316
2005 The Set1 methyltransferase opposes Ipl1 aurora kinase functions in chromosome segregation. Cell 125 16143104
1999 Sli15 associates with the ipl1 protein kinase to promote proper chromosome segregation in Saccharomyces cerevisiae. The Journal of cell biology 119 10385519
2020 The Long Noncoding RNA CCAT2 Induces Chromosomal Instability Through BOP1-AURKB Signaling. Gastroenterology 113 32805281
2019 AURKB as a target in non-small cell lung cancer with acquired resistance to anti-EGFR therapy. Nature communications 104 31000705
2003 An Mtw1 complex promotes kinetochore biorientation that is monitored by the Ipl1/Aurora protein kinase. Developmental cell 96 14602074
2006 Glc7/protein phosphatase 1 regulatory subunits can oppose the Ipl1/aurora protein kinase by redistributing Glc7. Molecular and cellular biology 93 16537909
1998 Cloning of STK13, a third human protein kinase related to Drosophila aurora and budding yeast Ipl1 that maps on chromosome 19q13.3-ter. Genomics 85 9799611
2019 The COMA complex interacts with Cse4 and positions Sli15/Ipl1 at the budding yeast inner kinetochore. eLife 67 31112132
2020 AURKB promotes gastric cancer progression via activation of CCND1 expression. Aging 65 31982864
2020 ALKBH5 promotes the proliferation of renal cell carcinoma by regulating AURKB expression in an m6A-dependent manner. Annals of translational medicine 63 32566583
2013 Mps1 and Ipl1/Aurora B act sequentially to correctly orient chromosomes on the meiotic spindle of budding yeast. Science (New York, N.Y.) 63 23371552
2018 Genetic Interactions between the Aurora Kinases Reveal New Requirements for AURKB and AURKC during Oocyte Meiosis. Current biology : CB 62 30415701
2010 High expression of AURKA and AURKB is associated with unfavorable cytogenetic abnormalities and high white blood cell count in patients with acute myeloid leukemia. Leukemia research 60 20732714
1998 Identification and characterization of STK12/Aik2: a human gene related to aurora of Drosophila and yeast IPL1. Cytogenetics and cell genetics 60 9858806
2007 A pathway containing the Ipl1/aurora protein kinase and the spindle midzone protein Ase1 regulates yeast spindle assembly. Developmental cell 59 17765685
2009 Analysis of Ipl1-mediated phosphorylation of the Ndc80 kinetochore protein in Saccharomyces cerevisiae. Genetics 57 19822728
2014 Sgo1 regulates both condensin and Ipl1/Aurora B to promote chromosome biorientation. PLoS genetics 56 24945276
2009 Ipl1-dependent phosphorylation of Dam1 is reduced by tension applied on kinetochores. Journal of cell science 56 19923271
1994 Regulation of yeast chromosome segregation by Ipl1 protein kinase and type 1 protein phosphatase. Cellular & molecular biology research 55 7874197
2014 Overcoming cetuximab resistance in HNSCC: the role of AURKB and DUSP proteins. Cancer letters 53 25192874
2009 RNAi-mediated knockdown of AURKB and EGFR shows enhanced therapeutic efficacy in prostate tumor regression. Gene therapy 49 19956271
2007 The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis. The Journal of cell biology 48 17371833
2019 Relation of AURKB over-expression to low survival rate in BCRA and reversine-modulated aurora B kinase in breast cancer cell lines. Cancer cell international 46 31244554
2023 PLK1 and AURKB phosphorylate survivin differentially to affect proliferation in racially distinct triple-negative breast cancer. Cell death & disease 43 36627281
2018 Let-7b attenuates cisplatin resistance and tumor growth in gastric cancer by targeting AURKB. Cancer gene therapy 43 30237418
2018 VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis. Cellular and molecular life sciences : CMLS 41 29340707
2009 AURKB-mediated effects on chromatin regulate binding versus release of XIST RNA to the inactive chromosome. The Journal of cell biology 41 19704020
2016 Aurkb/PP1-mediated resetting of Oct4 during the cell cycle determines the identity of embryonic stem cells. eLife 40 26880562
2019 AURKB: a promising biomarker in clear cell renal cell carcinoma. PeerJ 39 31576249
2011 Ipl1/Aurora-dependent phosphorylation of Sli15/INCENP regulates CPC-spindle interaction to ensure proper microtubule dynamics. The Journal of cell biology 39 21727193
2010 System-level analysis of neuroblastoma tumor-initiating cells implicates AURKB as a novel drug target for neuroblastoma. Clinical cancer research : an official journal of the American Association for Cancer Research 39 20651058
2016 Inhibition of Aurora kinases induces apoptosis and autophagy via AURKB/p70S6K/RPL15 axis in human leukemia cells. Cancer letters 38 27612557
2021 Mitotic syndicates Aurora Kinase B (AURKB) and mitotic arrest deficient 2 like 2 (MAD2L2) in cohorts of DNA damage response (DDR) and tumorigenesis. Mutation research. Reviews in mutation research 36 34083040
2021 BRAFV600E induces reversible mitotic arrest in human melanocytes via microrna-mediated suppression of AURKB. eLife 36 34812139
2018 Down-regulated let-7b-5p represses glycolysis metabolism by targeting AURKB in asthenozoospermia. Gene 36 29653228
2009 Ipl1/Aurora B kinase coordinates synaptonemal complex disassembly with cell cycle progression and crossover formation in budding yeast meiosis. Genes & development 35 19759266
2019 Relapse-associated AURKB blunts the glucocorticoid sensitivity of B cell acute lymphoblastic leukemia. Proceedings of the National Academy of Sciences of the United States of America 34 30733284
2013 Bcr-Abl activates AURKA and AURKB in chronic myeloid leukemia cells via AKT signaling. International journal of cancer 33 23934627
2009 Nbl1p: a Borealin/Dasra/CSC-1-like protein essential for Aurora/Ipl1 complex function and integrity in Saccharomyces cerevisiae. Molecular biology of the cell 31 19158380
2020 AURKB Promotes the Metastasis of Gastric Cancer, Possibly by Inducing EMT. Cancer management and research 30 32801915
2015 The potential role of the NEK6, AURKA, AURKB, and PAK1 genes in adenomatous colorectal polyps and colorectal adenocarcinoma. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 29 26423403
2011 The Aurora kinase Ipl1 is necessary for spindle pole body cohesion during budding yeast meiosis. Journal of cell science 29 21878496
2023 Plasmodium ARK2 and EB1 drive unconventional spindle dynamics, during chromosome segregation in sexual transmission stages. Nature communications 28 37704606
2015 Ipl1/Aurora-B is necessary for kinetochore restructuring in meiosis I in Saccharomyces cerevisiae. Molecular biology of the cell 28 26157162
2019 Viral-Mediated AURKB Cleavage Promotes Cell Segregation and Tumorigenesis. Cell reports 27 30917319
2012 Spatiotemporal regulation of Ipl1/Aurora activity by direct Cdk1 phosphorylation. Current biology : CB 25 22521784
2022 LncRNA PVT1 contributes to invasion and doxorubicin resistance of bladder cancer cells through promoting MDM2 expression and AURKB-mediated p53 ubiquitination. Environmental toxicology 24 35213076
2014 BUB1 mRNA is significantly co-expressed with AURKA and AURKB mRNA in advanced-stage ovarian serous carcinoma. Virchows Archiv : an international journal of pathology 24 24756216
2011 Temperature-sensitive ipl1-2/Aurora B mutation is suppressed by mutations in TOR complex 1 via the Glc7/PP1 phosphatase. Proceedings of the National Academy of Sciences of the United States of America 24 21368139
2019 Combined elevation of AURKB and UBE2C predicts severe outcomes and therapy resistance in glioma. Pathology, research and practice 23 31353228
2012 Differential expression of AURKA and AURKB genes in bone marrow stromal mesenchymal cells of myelodysplastic syndrome: correlation with G-banding analysis and FISH. Experimental hematology 23 23092930
2024 AURKB promotes bladder cancer progression by deregulating the p53 DNA damage response pathway via MAD2L2. Journal of translational medicine 22 38515112
2018 Polymorphisms in AURKA and AURKB are associated with the survival of triple-negative breast cancer patients treated with taxane-based adjuvant chemotherapy. Cancer management and research 22 30288111
2014 Loss-of-function RNAi screens in breast cancer cells identify AURKB, PLK1, PIK3R1, MAPK12, PRKD2, and PTK6 as sensitizing targets of rapamycin activity. Cancer letters 22 25193464
2020 HI-511 overcomes melanoma drug resistance via targeting AURKB and BRAF V600E. Theranostics 21 32863956
2018 MGMT inhibition in ER positive breast cancer leads to CDC2, TOP2A, AURKB, CDC20, KIF20A, Cyclin A2, Cyclin B2, Cyclin D1, ERα and Survivin inhibition and enhances response to temozolomide. Oncotarget 21 30038716
1999 Cloning, mapping, and expression of ial, a novel Drosophila member of the Ipl1/aurora mitotic control kinase family. DNA and cell biology 20 10433558
2023 BID expression determines the apoptotic fate of cancer cells after abrogation of the spindle assembly checkpoint by AURKB or TTK inhibitors. Molecular cancer 19 37443114
2023 AURKB activates EMT through PI3K/AKT signaling axis to promote ICC progression. Discover oncology 17 37318676
1999 The Xenopus laevis centrosome aurora/Ipl1-related kinase. Biology of the cell 17 10519006
2019 Spatio-temporal regulation of nuclear division by Aurora B kinase Ipl1 in Cryptococcus neoformans. PLoS genetics 16 30763303
2024 AURKB targets DHX9 to promote hepatocellular carcinoma progression via PI3K/AKT/mTOR pathway. Molecular carcinogenesis 14 38874176
2024 RNA demethylase FTO participates in malignant progression of gastric cancer by regulating SP1-AURKB-ATM pathway. Communications biology 14 38956367
2017 Rad52 phosphorylation by Ipl1 and Mps1 contributes to Mps1 kinetochore localization and spindle assembly checkpoint regulation. Proceedings of the National Academy of Sciences of the United States of America 14 29078282
2012 AURKB and MAPK involvement in the regulation of the early stages of mouse zygote development. Science China. Life sciences 14 22314491
2018 Potential Molecular Mechanisms of AURKB in the Oncogenesis and Progression of Osteosarcoma Cells: A Label-Free Quantitative Proteomics Analysis. Technology in cancer research & treatment 13 31122179
2017 The phosphorylation of a kinetochore protein Dam1 by Aurora B/Ipl1 kinase promotes chromosome bipolar attachment in yeast. Scientific reports 13 28928489
2012 Mutation screening of AURKB and SYCP3 in patients with reproductive problems. Molecular human reproduction 13 23100464
2023 Molecular imaging of HER2 expression in breast cancer patients using a novel peptide-based tracer 99mTc-HP-Ark2: a pilot study. Journal of translational medicine 12 36631812
2022 Effects of Shenkang Pills on Early-Stage Diabetic Nephropathy in db/db Mice via Inhibiting AURKB/RacGAP1/RhoA Signaling Pathway. Frontiers in pharmacology 12 35222021
2024 USP29 activation mediated by FUBP1 promotes AURKB stability and oncogenic functions in gastric cancer. Cancer cell international 11 38233848
2022 Haspin participates in AURKB recruitment to centromeres and contributes to chromosome congression in male mouse meiosis. Journal of cell science 11 35694956
2017 Haspin inhibition reveals functional differences of interchromatid axis-localized AURKB and AURKC. Molecular biology of the cell 11 28659416
2017 Single nucleotide polymorphisms rs911160 in AURKA and rs2289590 in AURKB mitotic checkpoint genes contribute to gastric cancer susceptibility. Environmental and molecular mutagenesis 11 28843004
2014 Phosphorylation of Sli15 by Ipl1 is important for proper CPC localization and chromosome stability in Saccharomyces cerevisiae. PloS one 11 24558497
2013 Ipl1/Aurora kinase suppresses S-CDK-driven spindle formation during prophase I to ensure chromosome integrity during meiosis. PloS one 11 24386320
2005 The Ipl1/Aurora kinase family: methods of inhibition and functional analysis in mammalian cells. Methods in molecular biology (Clifton, N.J.) 11 15576945
2024 AURKB promotes colorectal cancer progression by triggering the phosphorylation of histone H3 at serine 10 to activate CCNE1 expression. Aging 10 38713155
2023 AURKB promotes tumorigenesis and carboplatin resistance by regulating the ERK pathway in neuroblastoma cells. The International journal of neuroscience 10 34396896
2023 AURKB Enhances Chromosomal Remodeling of Telomeric Genes and Accelerates Tumorigenesis of Uveal Melanoma. Investigative ophthalmology & visual science 10 37079315
2012 Suppressors of ipl1-2 in components of a Glc7 phosphatase complex, Cdc48 AAA ATPase, TORC1, and the kinetochore. G3 (Bethesda, Md.) 10 23275890
2024 Inhibition of Aurora B kinase (AURKB) enhances the effectiveness of 5-fluorouracil chemotherapy against colorectal cancer cells. British journal of cancer 9 38287178
2023 Barasertib impedes chondrocyte senescence and alleviates osteoarthritis by mitigating the destabilization of heterochromatin induced by AURKB. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 9 37634474
2021 Multi-omics analysis identifies potential mechanisms of AURKB in mediating poor outcome of lung adenocarcinoma. Aging 9 33612479
1999 In silico cloning of a new protein kinase, Aik2, related to Drosophila Aurora using the new tool: EST Blast. In silico biology 9 11471245
2025 Histone lactylation-boosted AURKB facilitates colorectal cancer progression by inhibiting HNRNPM-mediated PSAT1 mRNA degradation. Journal of experimental & clinical cancer research : CR 8 40784984

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