Affinage

ASIC3

Acid-sensing ion channel 3 · UniProt Q9UHC3

Length
531 aa
Mass
58.9 kDa
Annotated
2026-06-09
100 papers in source corpus 34 papers cited in narrative 34 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ASIC3 is a proton-gated, Na+-selective cation channel of the DEG/ENaC superfamily that functions in peripheral sensory neurons as a detector of extracellular acidosis and contributes to nociception, mechanosensation, and chemosensation (PMID:11754838, PMID:12037186). It distinctively produces a sustained inward current within the mild pH range (7.3–6.7) characteristic of cardiac and skeletal muscle ischemia, because its activation and inactivation curves overlap in this window; lactate, Zn2+, and amiloride further modulate this sustained mode (PMID:16873722). ASIC3 rarely acts as a homomer in vivo: it co-assembles into heteromeric channels with ASIC2a and ASIC2b that determine current amplitude, kinetics, pH sensitivity, and surface expression (PMID:10842183, PMID:19590043, PMID:23109675, PMID:27241858), and genetic dissection in cardiac and skeletal muscle afferents confirms ASIC2a/3 (and ASIC1a/2a/3) heteromers as the principal native channels (PMID:19590043, PMID:23109675). Channel activity is tuned by intracellular scaffolds and kinases—PICK-1 enables PKC-dependent potentiation of ASIC3/ASIC2b channels, PSD-95 within lipid rafts reduces surface expression and current, and CAR modulates trafficking through this PDZ-based complex (PMID:14976185, PMID:18579798, PMID:22809504)—and is further sensitized by PAR2 signaling through a G protein/PLC/PKC/PKA cascade (PMID:28754162). In sensory physiology, muscle ASIC3 drives inflammatory mechanical hyperalgesia and ischemic myalgia via an IL-1β→IL-1R axis (PMID:17134831, PMID:27358445), contributes to proprioceptor and muscle-spindle dynamic sensitivity (PMID:27161260), mediates immediate exercise-induced pain while protecting against muscle injury (PMID:37795390), and mediates acid-plus-nonproton-ligand coincident detection underlying itch (PMID:26440887). Beyond neurons, ASIC3 acts via Ca2+/RhoA signaling in pancreatic cancer EMT and metastasis (PMID:28518134) and drives CGRP-dependent neurogenic inflammation in psoriasis (PMID:38902277). ASIC3 expression is transcriptionally controlled by SMAD3 repression, p75NTR/ERK maintenance, and estrogen-receptor-α induction (PMID:18466073, PMID:17696763, PMID:30114476). Pharmacologically, the sea anemone peptide APETx2 selectively inhibits ASIC3, RFamide peptides potentiate it, and amiloride paradoxically potentiates the sustained current at neutral pH through the nonproton ligand-sensing domain (PMID:15044953, PMID:28396446, PMID:34721074).

Mechanistic history

Synthesis pass · year-by-year structured walk · 34 steps
  1. 2000 High

    Established that ASIC3 is not an obligate homomer but co-assembles with ASIC2a into proton-gated heteromers, answering how subunit composition sets channel properties.

    Evidence Xenopus oocyte co-expression electrophysiology plus co-purification from HEK293 cells

    PMID:10842183

    Open questions at the time
    • Stoichiometry of the heteromer not resolved
    • Did not establish native composition in sensory neurons
  2. 2001 High

    Defined ASIC3's in vivo sensory role by showing its loss bidirectionally alters mechanoreceptor and acid/heat nociceptor sensitivity, implicating it in heteromeric channels in sensory endings.

    Evidence DRASIC-/- knockout mouse with skin immunolocalization and single-fiber recordings

    PMID:11754838

    Open questions at the time
    • Did not identify the specific heteromeric partners in each fiber type
    • Mechanism of mechanoreceptor modulation unresolved
  3. 2002 High

    Showed ASIC3 shapes the kinetics, pH sensitivity, and pharmacology of native DRG H+-gated currents, confirming it forms heteromultimeric acid-sensors in sensory neurons.

    Evidence Whole-cell patch clamp of large DRG neurons from DRASIC-/- vs wild-type mice

    PMID:12037186

    Open questions at the time
    • Partner subunits in large DRG neurons not biochemically defined
  4. 2004 High

    Identified APETx2 as a selective ASIC3 antagonist, providing the key pharmacological tool to probe native ASIC3 currents.

    Evidence Electrophysiology in oocytes, HEK cells, and rat DRG neurons with outside-out patches

    PMID:15044953

    Open questions at the time
    • Binding site on ASIC3 not mapped
    • Cross-reactivity in mixed heteromers incompletely characterized
  5. 2004 High

    Resolved how kinase signaling tunes ASIC3 by showing PKC potentiates ASIC3/ASIC2b channels via the PICK-1 scaffold binding the ASIC2b C-terminus.

    Evidence Patch clamp of DRG and heterologous cells with PKC activation and PDZ-domain interaction analysis

    PMID:14976185

    Open questions at the time
    • Phosphorylation site on the channel not identified
    • Whether PKC acts directly on ASIC3 or via PICK-1 unresolved
  6. 2004 High

    Distinguished cell-body mechanotransduction from sensory-ending function by showing ASIC2/ASIC3 are dispensable for mechanically activated currents in DRG somata.

    Evidence Patch clamp of cultured DRG neurons from single and double KO mice with mechanical stimulation

    PMID:14990679

    Open questions at the time
    • Negative result does not exclude a role at peripheral endings
    • Possible compensation by other channels not addressed
  7. 2006 High

    Explained ASIC3's ischemia-sensing capacity by demonstrating a sustained current in the mild pH range where activation and inactivation overlap, enhanced in ASIC2a/3 heteromers.

    Evidence Patch clamp of transfected lines and cardiac sensory neurons with pH dose-response and pharmacology

    PMID:16873722

    Open questions at the time
    • Molecular basis of overlapping activation/inactivation curves not structurally defined
    • Contribution of endogenous lactate in vivo not quantified
  8. 2006 High

    Localized the critical site of inflammatory mechanical hyperalgesia to muscle ASIC3 using tissue-specific genetic rescue.

    Evidence ASIC3-/- mice with herpesvirus-mediated muscle-specific re-expression and behavioral testing

    PMID:17134831

    Open questions at the time
    • Downstream central sensitization mechanism not defined
    • Did not identify the proton/inflammatory trigger in muscle
  9. 2007 Medium

    Linked ASIC3 expression and cell survival in nucleus pulposus to p75NTR/ERK signaling, extending ASIC3 function beyond neurons.

    Evidence Luciferase reporters, dominant-negative/constitutively active MEK, p75NTR blockade, caspase-3 assays

    PMID:17696763

    Open questions at the time
    • Single-lab reporter-based study
    • Direct transcription factor binding not shown
  10. 2008 High

    Established transcriptional repression of ASIC3 by SMAD3 downstream of TGF-β, defining a direct promoter-level control mechanism.

    Evidence Reporter assays, EMSA/supershift, ChIP, and smad3-null cell rescue in nucleus pulposus cells

    PMID:18466073

    Open questions at the time
    • Relevance to neuronal ASIC3 regulation not tested
    • HDAC partner identity not specified
  11. 2008 High

    Showed PSD-95 within lipid rafts is a negative regulator of ASIC3 surface expression and current, revealing membrane-microdomain control.

    Evidence Co-IP, detergent-resistant membrane fractionation, cholesterol depletion, PSD-95 palmitoylation mutagenesis, patch clamp

    PMID:18579798

    Open questions at the time
    • In vivo relevance in sensory neurons not established
    • Mechanism by which raft localization reduces current unclear
  12. 2009 High

    Defined the major native cardiac afferent ASIC as the ASIC2a/3 heteromer using complementary genetic ablations.

    Evidence Retrograde labeling and patch clamp in cardiac afferents from ASIC2-/- and ASIC3-/- mice

    PMID:19590043

    Open questions at the time
    • Heteromer stoichiometry not determined
    • Contribution to cardiac pain perception in vivo not directly measured here
  13. 2010 High

    Identified ASIC3 as a specific molecular determinant of acidosis chemotransduction in carotid body glomus cells via reciprocal gain/loss of function.

    Evidence Ca2+ imaging in glomus cells from ASIC3 transgenic and KO mice with pH and hypoxia stimulation

    PMID:23165770

    Open questions at the time
    • Heteromeric partners in glomus cells not defined
    • Link to ventilatory output not directly tested
  14. 2012 High

    Demonstrated ASIC1a/2a/3 heteromers are the principal acid sensors of skeletal muscle afferents through systematic single and triple knockouts.

    Evidence Patch clamp of labeled muscle afferents from multiple ASIC-null genotypes

    PMID:23109675

    Open questions at the time
    • Subunit ratio within native heteromers unknown
  15. 2012 High

    Clarified that ASIC subunits influence but do not directly transduce cutaneous mechanical stimuli, since triple-KO increases mechanosensitivity.

    Evidence Triple-KO von Frey behavior and single-fiber cutaneous recordings

    PMID:22506072

    Open questions at the time
    • Identity of the actual mechanotransducer remains open
    • Mechanism of the modulatory effect unresolved
  16. 2012 Medium

    Showed CAR recruits ASIC3 and PSD-95 to cell-cell junctions and can rescue PSD-95-mediated inhibition, adding a trafficking layer to the PDZ scaffold network.

    Evidence Co-IP, immunofluorescence, and patch clamp in heterologous cells

    PMID:22809504

    Open questions at the time
    • Heterologous system only
    • Physiological context in sensory neurons untested
  17. 2012 Medium

    Provided in vivo evidence for ASIC3 in osteoarthritic joint pain and a candidate chondroprotective role using APETx2 blockade.

    Evidence Immunohistochemistry, behavioral pain testing, and intra-articular APETx2 in a monoiodoacetate OA model

    PMID:22909215

    Open questions at the time
    • Single-lab pharmacology
    • Cellular mechanism of chondroprotection unclear
  18. 2013 High

    Characterized Ugr 9-1 as a structurally novel ASIC3 inhibitor that differentially blocks transient versus sustained current components.

    Evidence Xenopus oocyte electrophysiology, NMR structure, and mouse pain assays

    PMID:23801332

    Open questions at the time
    • Binding site on ASIC3 not mapped
    • Selectivity across heteromers not fully tested
  19. 2015 High

    Revealed ASIC3 mediates itch via coincident detection of acid and the nonproton ligand SL-NH2 acting on an extracellular ligand-sensing domain.

    Evidence ASIC3-/- scratching behavior, patch clamp, and nonproton ligand-sensing domain mutagenesis

    PMID:26440887

    Open questions at the time
    • Endogenous nonproton ligand in itch not identified
    • Downstream itch circuitry not defined
  20. 2016 High

    Established muscle ASIC3 as a node in an IL-1β→IL-1R axis driving ischemic myalgia through afferent sensitization.

    Evidence IL-1β treatment of single DRG neurons, nerve-specific siRNA knockdown, single-unit recordings, behavior

    PMID:27358445

    Open questions at the time
    • Transcriptional mechanism of IL-1β-induced ASIC3 upregulation not defined
  21. 2016 High

    Defined ASIC3 in proprioceptor axons as a contributor to muscle-spindle dynamic sensitivity and stretch-dependent mechanotransduction.

    Evidence Asic3 conditional KO in Pv+ neurons, spindle afferent electrophysiology, elastic-matrix stimulation, behavior

    PMID:27161260

    Open questions at the time
    • Whether ASIC3 directly transduces stretch or modulates a transducer unresolved
  22. 2016 High

    Demonstrated dual mechano- and chemosensory ASIC3 function in lamprey CSF-contacting neurons using APETx2 blockade.

    Evidence Patch clamp of identified CSF-contacting neurons with APETx2, mechanical bending, and pH manipulation

    PMID:26743691

    Open questions at the time
    • Mechanism of mechanical gating in these neurons not defined
    • Conservation to mammalian CSF-contacting neurons not shown
  23. 2016 Medium

    Showed ASIC2a co-expression rescues ASIC3 from ER retention, driving surface targeting and enhancing the sustained current.

    Evidence Surface biotinylation, BiFC, and patch clamp in heterologous cells

    PMID:27241858

    Open questions at the time
    • Heterologous system only
    • ER retention signal on ASIC3 not mapped
  24. 2017 High

    Identified a physical and functional ASIC3–P2X3 complex in which ASIC3 activation inhibits P2X3 current via a Ca2+-dependent mechanism.

    Evidence Patch clamp, reciprocal Co-IP, BN-PAGE/SDS-PAGE, Ca2+ chelation, in vivo pain summation

    PMID:29636447

    Open questions at the time
    • Structural basis of the complex unresolved
    • Not a single heteromeric channel; interface undefined
  25. 2017 High

    Characterized the cono-RFamide RPRFamide as a potentiator of ASIC3 that enhances acid-induced muscle pain in an ASIC3-dependent manner.

    Evidence Venom electrophysiology, DRG patch clamp, and ASIC3-/- in vivo pain assay

    PMID:28396446

    Open questions at the time
    • Binding site for RFamide peptides not mapped
    • Endogenous mammalian RFamide relevance not addressed
  26. 2017 High

    Defined PAR2 as a sensitizer of ASIC3 acting through a G protein/PLC/PKC/PKA cascade in heterologous and native neurons.

    Evidence Patch clamp in CHO cells and DRG neurons with stepwise pharmacological inhibition

    PMID:28754162

    Open questions at the time
    • Direct phosphorylation target on ASIC3 not identified
  27. 2017 Medium

    Extended ASIC3 function to cancer biology, showing ASIC1/ASIC3 mediate acidity-induced EMT and metastasis via a Ca2+/RhoA pathway.

    Evidence siRNA knockdown, pharmacological inhibition, Ca2+ imaging, RhoA assay, xenograft metastasis

    PMID:28518134

    Open questions at the time
    • Single-lab study
    • Relative contribution of ASIC1 vs ASIC3 not dissected
  28. 2018 Medium

    Connected ASIC3 to sex-dependent expression by showing estrogen-receptor-α-mediated upregulation of ASIC3 protein.

    Evidence Co-transfection western blot with ERα antagonist and in vivo ovariectomy/orchiectomy

    PMID:30114476

    Open questions at the time
    • Whether ERα acts at the ASIC3 promoter directly not shown
    • Functional consequence for pain not measured here
  29. 2019 Medium

    Implicated ASIC3 in hypoxic nucleus pulposus cell fate through interaction with HIF-1α and ERK1/2 and downstream MAPK/ERK-driven apoptosis and autophagy.

    Evidence Co-IP, shRNA knockdown, overexpression, flow cytometry, western blot, EM

    PMID:31202172

    Open questions at the time
    • Single-lab correlative interaction data
    • Direct vs indirect HIF-1α interaction unclear
  30. 2021 High

    Mechanistically dissected amiloride's paradoxical potentiation of ASIC3 sustained current at neutral pH, mapping it to nonproton ligand-sensing residues E79/E423 and the pore site G445.

    Evidence Patch clamp with systematic site-directed mutagenesis in heterologous cells

    PMID:34721074

    Open questions at the time
    • Structural model of dual amiloride action not resolved
    • Physiological relevance of neutral-pH potentiation untested
  31. 2022 Medium

    Implicated ASIC3 in skin fibrosis through an ASIC3–M-CSF–TGF-β1 positive feedback loop driving M2 macrophage polarization and myofibroblast differentiation.

    Evidence ASIC3 activation/inhibition in cultured cells, macrophage polarization and fibroblast differentiation assays

    PMID:35661105

    Open questions at the time
    • Primarily in vitro
    • How acid activation occurs in fibrotic tissue not defined
  32. 2023 Medium

    Defined a dual role for ASIC3 in exercise: required for immediate exercise-induced pain but protective against strenuous-exercise muscle injury.

    Evidence ASIC3-/- mice with treadmill exercise, withdrawal threshold, locomotor and grip testing, serum injury markers

    PMID:37795390

    Open questions at the time
    • Mechanism of protection against muscle injury not defined
    • Single-lab study
  33. 2023 Low

    Predicted a transmembrane arachidonic-acid binding site on ASIC3 involving R65, R68, and Y58, proposing lipid modulation of the channel.

    Evidence Multiscale molecular dynamics simulations with in silico mutagenesis

    PMID:36625864

    Open questions at the time
    • Computational prediction without experimental functional validation
    • Functional effect of AA binding on ASIC3 not measured
  34. 2024 High

    Established ASIC3 as a driver of neurogenic psoriatic inflammation through activity-dependent CGRP release from nociceptors.

    Evidence Global and nociceptor-specific conditional KO, imiquimod psoriasis model, CGRP rescue, botulinum toxin, CGRP antagonists

    PMID:38902277

    Open questions at the time
    • Endogenous acidic/ligand trigger in psoriatic skin not defined
    • Sex-specificity of the effect not fully explained

Open questions

Synthesis pass · forward-looking unresolved questions
  • A high-resolution structural basis for ASIC3's overlapping activation/inactivation, heteromer interfaces, and the nonproton ligand-sensing domain that integrates protons, RFamides, and amiloride remains undefined.
  • No experimental structure of ASIC3 or its heteromers
  • Subunit stoichiometry of native heteromers unresolved
  • Direct phosphorylation sites mediating kinase potentiation unmapped

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005215 transporter activity 3 GO:0140299 molecular sensor activity 3
Localization
GO:0005886 plasma membrane 3 GO:0005783 endoplasmic reticulum 1
Pathway
R-HSA-112316 Neuronal System 3 R-HSA-162582 Signal Transduction 3 R-HSA-9709957 Sensory Perception 3
Partners
ASIC1AASIC2AASIC2BCXADRDLG4HIF1AP2RX3PICK1
Complex memberships
ASIC1a/ASIC2a/ASIC3 heteromeric channelASIC2a/ASIC3 heteromeric channelASIC3/ASIC2b/PICK-1 complexASIC3–P2X3 multiprotein complex

Evidence

Reading pass · 34 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 DRASIC (ASIC3) is localized in multiple specialized sensory nerve endings in skin, and its genetic disruption alters mechanoreceptor sensitivity bidirectionally: loss of DRASIC increases sensitivity of light-touch mechanoreceptors but reduces sensitivity of noxious-pinch mechanoreceptors and acid/noxious-heat nociceptors. The data indicate DRASIC participates in heteromultimeric channel complexes in sensory neurons. Gene knockout mouse (DRASIC-/−), immunolocalization in skin nerve endings, single-fiber electrophysiology Neuron High 11754838
2000 ASIC2a and ASIC3 subunits co-assemble into heteromeric proton-gated cation channels: co-expression in Xenopus oocytes produces currents up to 20-fold larger than homomers, with a reversal potential reflecting Na+-selective current, and co-purification from HEK293 cells confirms biochemical interaction. Heteromeric ASIC2a/3 channels show increased sensitivity to Gd3+ (IC50 ~40 µM) compared to ASIC2a homomers (IC50 ≥1 mM). Xenopus oocyte co-expression electrophysiology, co-purification/co-immunoprecipitation from HEK293 cells, in situ hybridization for co-localization The Journal of biological chemistry High 10842183
2002 DRASIC (ASIC3) contributes to the composition and properties of H+-gated currents in large-diameter DRG neurons: genetic disruption of DRASIC slows desensitization kinetics, decreases pH sensitivity, increases amiloride sensitivity, and alters FMRF-related peptide potentiation, indicating DRASIC forms heteromultimeric acid-activated channels with other DEG/ENaC subunits in these neurons. Whole-cell patch clamp on large DRG neurons from DRASIC-/− mice vs. wild-type Journal of neurophysiology High 12037186
2004 APETx2, a 42-amino-acid peptide from sea anemone Anthopleura elegantissima, acts at the extracellular face of ASIC3 to reversibly inhibit rat ASIC3 homomeric channels (IC50 = 63 nM) and ASIC3-containing heteromeric channels, without affecting ASIC1a, ASIC1b, or ASIC2a. It does not change unitary conductance. It also inhibits the native ASIC3-like current in rat DRG neurons (IC50 = 216 nM). Electrophysiology in heterologous expression systems (Xenopus oocytes, HEK cells) and primary DRG neuron patch clamp; pharmacological characterization with outside-out patches The EMBO journal High 15044953
2004 ASIC3 is regulated by protein kinase C (PKC) through its interaction with the silent ASIC2b subunit and the PDZ scaffold protein PICK-1. PKC stimulation (via phorbol ester PDBu or serotonin) increases native ASIC3-like currents in DRG neurons and shifts pH dependence toward more physiological values only in heteromeric ASIC3/ASIC2b channels (not ASIC3 homomers), requiring PICK-1 which binds the ASIC2b C-terminus. Whole-cell patch clamp of rat DRG neurons and heterologous expression, pharmacological PKC activation, PICK-1 interaction identified by PDZ domain analysis The Journal of biological chemistry High 14976185
2004 ASIC2 and ASIC3 null mutations do not alter mechanically activated currents (amplitude or kinetics) in isolated DRG neuron cell bodies, indicating that ASIC2 and ASIC3 are not required for mechanotransduction at the cell body level. Whole-cell patch clamp of cultured DRG neurons from ASIC2-/−, ASIC3-/−, and double-KO mice with mechanical stimulation The Journal of physiology High 14990679
2006 ASIC3 produces a sustained inward current within the pH range (7.3–6.7) that occurs during cardiac/skeletal muscle ischemia, because activation and inactivation curves overlap in this range. This sustained mode does not occur with ASIC1a homomers or ASIC1a/3 heteromers; ASIC2a/3 heteromers produce larger sustained currents than ASIC3 homomers. Lactate shifts activation to more basic pH; amiloride paradoxically increases ASIC3 current at pH 7.0. Cardiac sensory neurons exhibit a small perfectly sustained current at pH 7.0–7.4 that is potentiated by Zn2+ and amiloride. Patch clamp on transfected cell lines and fluorescently tagged cardiac sensory neurons; pH dose-response curves; pharmacological modulators Circulation research High 16873722
2006 ASIC3 in primary afferent fibers innervating muscle (not skin) is required for development of cutaneous mechanical hyperalgesia after carrageenan-induced muscle inflammation. Rescue of the phenotype by herpes virus-mediated ASIC3 expression specifically in muscle (not skin) of ASIC3-/− mice restores mechanical hyperalgesia, establishing peripheral muscle ASIC3 as the critical locus. ASIC3-/− mice, behavioral pain testing (mechanical and heat), herpes virus vector-mediated tissue-specific ASIC3 re-expression, DRG mRNA/protein confirmation Pain High 17134831
2008 PSD-95 interacts with ASIC3 via its PDZ domain and reduces ASIC3 cell surface expression and H+-gated current. ASIC3 and PSD-95 co-immunoprecipitate within lipid raft (detergent-resistant membrane) fractions; disruption of lipid rafts with methyl-β-cyclodextrin abolishes PSD-95 inhibition of ASIC3. Mutation of palmitoylation sites (Cys residues) in PSD-95 prevents its targeting to lipid rafts and its inhibition of ASIC3. Cell surface ASIC3 is enriched in lipid raft fractions. Co-immunoprecipitation, detergent-resistant membrane fractionation, methyl-β-cyclodextrin cholesterol depletion, site-directed mutagenesis of PSD-95 palmitoylation sites, whole-cell patch clamp American journal of physiology. Cell physiology High 18579798
2008 SMAD3 acts as a transcriptional repressor of the ASIC3 gene in nucleus pulposus cells. TGF-β treatment decreases ASIC3 mRNA/protein; constitutively active ALK5 or SMAD3 suppresses ASIC3 promoter activity; dominant-negative SMAD3 or SMAD7 restores it. SMAD3 directly binds two CAGA box motifs in the rat ASIC3 promoter as shown by gel-shift, supershift, and chromatin immunoprecipitation assays. Suppression requires histone deacetylase recruitment. Luciferase reporter assays, dominant-negative/constitutively active constructs, EMSA/supershift, ChIP, smad3-null cell rescue Journal of bone and mineral research High 18466073
2007 p75NTR and ERK signaling maintain basal ASIC3 expression in nucleus pulposus cells: blocking p75NTR suppresses basal ASIC3 promoter activity; dominant-negative MEK suppresses, while constitutively active MEK1 increases, ASIC3 promoter activity. ASIC3 promotes disc cell survival under acidic and hyperosmotic conditions by lowering caspase-3 activity. Luciferase reporter assays, dominant-negative/constitutively active MEK, p75NTR blocking antibody/dominant-negative construct, MTT and caspase-3 activity assays Journal of bone and mineral research Medium 17696763
2009 ASIC2a and ASIC3 are the major ASIC subunits forming heteromers in cardiac dorsal root ganglia neurons. Patch-clamp studies in cardiac afferents from ASIC3-/− mice show currents matching ASIC2a homomers; ASIC2-/− cardiac afferents show currents matching ASIC3 channels. Current properties of wild-type cardiac DRG neurons most closely match ASIC2a/3 heteromeric channels. Retrograde labeling + patch clamp in cardiac afferents from ASIC-null mice; pharmacological and kinetic characterization Circulation research High 19590043
2012 ASIC1a, ASIC2a, and ASIC3 form heteromeric channels as the principal ASICs in skeletal muscle afferents. ASIC1a-/− currents show reduced pH sensitivity and faster recovery; ASIC2-/− currents show diminished Zn2+ potentiation; ASIC3-/− currents show slower desensitization. ASIC-like currents are absent in triple-null (ASIC1a/2a/3) mice. Patch clamp of isolated labeled mouse muscle afferents from specific ASIC-/− mice; pharmacological modulation FASEB journal High 23109675
2012 Simultaneous knockout of ASIC1a, ASIC2, and ASIC3 (triple-KO) increases cutaneous mechanosensitivity: triple-KO mice show increased paw withdrawal frequency to von Frey filaments and enhanced single-fiber A-mechanonociceptor responses compared to wild-type. Other fiber types are unaffected. This indicates ASIC subunits influence mechanosensitivity but likely do not directly transduce mechanical stimuli. Triple-KO behavioral testing (von Frey), single-fiber cutaneous nerve recordings PloS one High 22506072
2016 ASIC3 is expressed in parvalbumin-positive (Pv+) proprioceptor axons innervating muscle spindles. Conditional knockout of ASIC3 in Pv+ neurons disrupts spindle afferent sensitivity to dynamic stimuli and impairs mechanotransduction in Pv+ DRG neurons in response to substrate deformation-induced neurite stretching, but not direct neurite indentation. Global and Pv-Cre::Asic3f/f mice show similar deficits in grid and balance beam walking tasks. Asic3-KO/eGFPf-knockin mice, Cre-lox conditional KO, electrophysiology of muscle spindle afferents, localized elastic matrix mechanostimulation, behavioral tasks Nature communications High 27161260
2016 ASIC3 in cerebrospinal fluid-contacting neurons of lamprey spinal cord mediates both mechanosensory and chemosensory (pH) responses. APETx2 (selective ASIC3 antagonist) blocks both mechanical stimulation-evoked and low-pH-evoked responses in these neurons. Lowering pH or applying somatostatin reduces locomotor burst rate; somatostatin receptor antagonist counteracts both effects. Whole-cell patch clamp of identified CSF-contacting neurons, APETx2 pharmacological blockade, imposed lateral bending, pH manipulation Nature communications High 26743691
2013 Sea anemone peptide Ugr 9-1 (π-AnmTX Ugr 9a-1) reversibly inhibits human ASIC3 expressed in Xenopus oocytes: it completely blocks the transient current component (IC50 10 µM) and partially inhibits the sustained component (IC50 1.44 µM). NMR spectroscopy revealed a novel 'boundless β-hairpin' structure stabilized by two disulfide bonds. Xenopus oocyte electrophysiology, NMR structure determination, in vivo pain assays in mice The Journal of biological chemistry High 23801332
2017 ASIC3 and P2X3 receptors physically associate to form a multiprotein complex in sensory neurons. ASIC3 stimulation strongly inhibits P2X3R current partly through a Ca2+-dependent mechanism. The proton-binding site of ASIC3 is critical for this inhibitory effect. Co-immunoprecipitation and BN-PAGE/SDS-PAGE analysis are consistent with a heteromeric multiprotein structure (though not a single heteromeric channel). The two receptors switch their ionic permeabilities during activation. Patch clamp current measurements, co-immunoprecipitation, BN-PAGE, SDS-PAGE, Ca2+ chelation experiments, in vivo pain summation in rats Nature communications High 29636447
2017 RPRFamide (a 4-amino acid cono-RFamide from Conus textile venom) strongly potentiates ASIC3 currents and increases DRG neuron excitability. Injection into mouse gastrocnemius muscle enhances acid-induced muscle pain, and this enhancement is abolished by genetic inactivation of ASIC3. In vitro venom screen electrophysiology, patch clamp of isolated DRG neurons, ASIC3-/− mouse in vivo pain assay Proceedings of the National Academy of Sciences of the United States of America High 28396446
2017 ASIC1 and ASIC3 mediate acidity-induced epithelial-mesenchymal transition (EMT) in pancreatic cancer cells through a Ca2+/RhoA pathway. Acid-induced inward current via ASIC1/ASIC3 elevates intracellular Ca2+, which activates RhoA downstream; siRNA knockdown or pharmacological inhibition of ASIC1/ASIC3 decreases [Ca2+]i and RhoA, suppressing EMT. ASIC1/ASIC3 knockdown suppresses liver and lung metastasis in xenograft models. siRNA knockdown, pharmacological inhibition, Ca2+ imaging, RhoA assay, xenograft metastasis model Cell death & disease Medium 28518134
2015 ASIC3 mediates itch sensation via coincident detection of acidosis and nonproton ligands (SL-NH2). Co-administration of acid with SL-NH2 increases scratching in wild-type but not ASIC3-null mice. Mechanistically, SL-NH2 slows desensitization of proton-evoked currents by targeting the nonproton ligand-sensing domain in the extracellular region of ASIC3. ASIC3 gene ablation also reduces dry-skin-induced scratching. ASIC3-/− behavioral assay, patch clamp on primary sensory neurons, mutagenesis of nonproton ligand-sensing domain Cell reports High 26440887
2017 PAR2 signaling sensitizes ASIC3 via an intracellular cascade involving G protein, PLC, PKC, and PKA. PAR2-activating peptide concentration-dependently increases ASIC3 currents in CHO cells expressing both ASIC3 and PAR2, increasing maximal response by ~59% without changing pH50. PAR2 activation also increases proton-evoked currents and spikes in rat DRG neurons. Inhibition of each signaling step (G protein, PLC, PKC, or PKA) abolishes PAR2-induced potentiation of ASIC3. Whole-cell patch clamp in CHO cells and DRG neurons, pharmacological inhibition of G protein/PLC/PKC/PKA Journal of neuroinflammation High 28754162
2016 ASIC2a co-expression increases ASIC3 surface expression via heteromeric assembly, markedly enhancing the sustained component of proton-evoked currents. ASIC3 alone is largely retained in the ER; co-expression with ASIC2a drives plasma membrane targeting. Heteromeric association confirmed by BiFC assay. Surface biotinylation, bimolecular fluorescence complementation (BiFC), whole-cell patch clamp in heterologous cells BMB reports Medium 27241858
2012 CAR (Coxsackievirus and adenovirus receptor) interacts with ASIC3 only when PSD-95 is co-expressed, and CAR recruits both PSD-95 and ASIC3 to cell-cell junctions. Furthermore, CAR rescues PSD-95-mediated inhibition of ASIC3 currents, indicating CAR can modulate ASIC3 trafficking through a PDZ-based scaffolding complex. Co-immunoprecipitation, immunofluorescence localization, whole-cell patch clamp in heterologous cells Biochemical and biophysical research communications Medium 22809504
2021 Amiloride exerts paradoxical potentiation of ASIC3 sustained currents at neutral pH while inhibiting currents at acidic pH. Mechanistically, amiloride causes alkaline shift of pH-dependent activation, inhibits steady-state desensitization (SSD), and requires extracellular Ca2+. Site-directed mutagenesis identifies E79 and E423 in the nonproton ligand-sensing domain as critical for amiloride-induced inhibition of SSD; disruption of the pore amiloride binding site (G445C) blunts both inhibition and potentiation. Whole-cell patch clamp, site-directed mutagenesis of ASIC3 expressed in heterologous cells Frontiers in physiology High 34721074
2023 Multiscale MD simulations predict arachidonic acid (AA) binds the outer leaflet transmembrane region of ASIC3, with salt bridge interactions at R65 and R68 and aromatic interactions at Y58 in hASIC3. Subtle differences exist between ASIC1a and ASIC3 AA interaction patterns reversible by mutating three key residues at the outer TM1 leaflet. Longer, more unsaturated fatty acid tails show increased occupancy at this region. Multiscale molecular dynamics simulations, mutagenesis analysis in silico The Journal of general physiology Low 36625864
2016 IL-1β from ischemic muscle upregulates ASIC3 in single muscle afferent neurons (DRG), and nerve-specific knockdown of ASIC3 recapitulates the effects of inhibiting IL1β/IL1R signaling, reversing sensitization of group III/IV afferents and pain behaviors after ischemia-reperfusion injury. This establishes an IL1β → IL1R → ASIC3 signaling axis in muscle afferents mediating ischemic myalgia. In vitro IL-1β treatment of single DRG neurons, siRNA nerve-specific knockdown in vivo, afferent single-unit recordings, behavioral pain assays The Journal of neuroscience High 27358445
2019 ASIC3 interacts with HIF-1α and ERK1/2 as shown by co-immunoprecipitation in nucleus pulposus cells. Hypoxia-induced ASIC3 upregulation is reduced by HIF-1α silencing, indicating mutual regulation. ASIC3 overexpression under hypoxia activates MAPK/ERK pathway, inhibits proliferation, arrests cell cycle in G1, and promotes apoptosis and autophagy; ASIC3 silencing reverses these effects. Co-immunoprecipitation, shRNA knockdown, ASIC3 overexpression, flow cytometry, western blot, CCK-8, transmission EM Biomedicine & pharmacotherapy Medium 31202172
2024 ASIC3 activation in nociceptors exacerbates psoriatic inflammation through a neurogenic pathway. Global or nociceptor-specific Asic3 KO in female mice alleviates imiquimod-induced psoriatic acanthosis and type 17 inflammation similarly to nociceptor ablation. Mechanistically, ASIC3 activation induces activity-dependent release of CGRP from sensory neurons to promote neurogenic inflammation; CGRP replenishment in skin of Asic3-KO mice restores the inflammatory response. Botulinum neurotoxin A and CGRP antagonists phenocopy Asic3 KO. Global and nociceptor-specific conditional KO, imiquimod-induced psoriasis model, CGRP rescue experiment, botulinum toxin, CGRP antagonists, histology Nature communications High 38902277
2022 ASIC3 activation promotes M-CSF transcriptional regulation in macrophages, driving M2 polarization. M2 macrophages transduced by the ASIC3-M-CSF signal promote fibroblast-to-myofibroblast differentiation through TGF-β1, forming an ASIC3-M-CSF-TGF-β1 positive feedback loop in skin fibrosis. ASIC3 activation/inhibition in cultured cells, western blot, cytokine/macrophage polarization assays, fibroblast differentiation assay Cell death & disease Medium 35661105
2008 17β-estradiol (E2) upregulates ASIC3 protein expression in a dose- and time-dependent manner via estrogen receptor α (ERα); the ERα-specific antagonist MPP blocks this effect. Female rats have higher ASIC3 expression in DRG than males; ovariectomy decreases DRG ASIC3 expression; orchiectomy does not affect it. Co-transfection of ASIC3 and ERα in 293T cells with western blot, ERα antagonist MPP, in vivo ovariectomy/orchiectomy with DRG western blot Neuroscience letters Medium 30114476
2012 ASIC3 expression is increased ~2-fold in knee joint afferents in rats with monoiodoacetate-induced osteoarthritis. Continuous intra-articular injection of APETx2 (ASIC3 blocker) inhibits weight-bearing pain, secondary hyperalgesia, and attenuates ASIC3 upregulation in joint afferents. Early APETx2 administration has chondroprotective effects. Immunohistochemistry, behavioral pain testing, intra-articular APETx2 injection, histology Journal of biomedical science Medium 22909215
2010 ASIC3-like current in carotid body glomus cells mediates acidosis chemosensitivity. Increased ASIC3 expression in transgenic mice increases pH sensitivity while reducing cyanide/hypoxia sensitivity; ASIC3 deletion reduces pH sensitivity while increasing relative sensitivity to hypoxia. This reciprocal relationship demonstrates ASIC3 is a specific molecular determinant of acidosis chemotransduction in glomus cells. Ca2+ imaging in isolated glomus cells, ASIC3 transgenic and KO mice, pharmacological and hypoxia stimulation The Journal of physiology High 23165770
2023 ASIC3 is required for immediate exercise-induced pain (IEIP) but plays a protective role against muscle injury during strenuous exercise. ASIC3-/− mice do not develop reduced muscle withdrawal threshold immediately after exhaustive exercise (no IEIP). At 24 h, ASIC3-/− mice show lower locomotor activity, lower repeat exercise performance, and higher serum markers of muscle injury (LDH, CK) than wild-type mice. ASIC3-/− mice, treadmill exercise, muscle withdrawal threshold, locomotor testing, grip strength, serum enzyme assays Frontiers in pain research Medium 37795390

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 The DRASIC cation channel contributes to the detection of cutaneous touch and acid stimuli in mice. Neuron 480 11754838
2004 A new sea anemone peptide, APETx2, inhibits ASIC3, a major acid-sensitive channel in sensory neurons. The EMBO journal 306 15044953
2002 A role for ASIC3 in the modulation of high-intensity pain stimuli. Proceedings of the National Academy of Sciences of the United States of America 259 12060708
2006 Sustained currents through ASIC3 ion channels at the modest pH changes that occur during myocardial ischemia. Circulation research 206 16873722
2004 Acid-sensing ion channels ASIC2 and ASIC3 do not contribute to mechanically activated currents in mammalian sensory neurones. The Journal of physiology 205 14990679
2005 ASIC3, an acid-sensing ion channel, is expressed in metaboreceptive sensory neurons. Molecular pain 195 16305749
2006 ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation. Pain 150 17134831
2000 Mammalian ASIC2a and ASIC3 subunits co-assemble into heteromeric proton-gated channels sensitive to Gd3+. The Journal of biological chemistry 140 10842183
2009 ASIC1 and ASIC3 play different roles in the development of Hyperalgesia after inflammatory muscle injury. The journal of pain 135 20015700
2008 Role of ASIC3 in the primary and secondary hyperalgesia produced by joint inflammation in mice. Pain 132 18343037
2016 Evidence for the involvement of ASIC3 in sensory mechanotransduction in proprioceptors. Nature communications 114 27161260
2005 Transgenic expression of a dominant-negative ASIC3 subunit leads to increased sensitivity to mechanical and inflammatory stimuli. The Journal of neuroscience : the official journal of the Society for Neuroscience 102 16251436
2012 Local ASIC3 modulates pain and disease progression in a rat model of osteoarthritis. Journal of biomedical science 93 22909215
2017 ASIC1 and ASIC3 contribute to acidity-induced EMT of pancreatic cancer through activating Ca2+/RhoA pathway. Cell death & disease 89 28518134
2016 Ciliated neurons lining the central canal sense both fluid movement and pH through ASIC3. Nature communications 85 26743691
2004 ASIC2b-dependent regulation of ASIC3, an essential acid-sensing ion channel subunit in sensory neurons via the partner protein PICK-1. The Journal of biological chemistry 84 14976185
2009 ASIC2a and ASIC3 heteromultimerize to form pH-sensitive channels in mouse cardiac dorsal root ganglia neurons. Circulation research 77 19590043
2017 Bone Pain Induced by Multiple Myeloma Is Reduced by Targeting V-ATPase and ASIC3. Cancer research 76 28254863
2007 Expression of acid-sensing ion channel 3 (ASIC3) in nucleus pulposus cells of the intervertebral disc is regulated by p75NTR and ERK signaling. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 72 17696763
2002 DRASIC contributes to pH-gated currents in large dorsal root ganglion sensory neurons by forming heteromultimeric channels. Journal of neurophysiology 68 12037186
2014 Roles of ASIC3, TRPV1, and NaV1.8 in the transition from acute to chronic pain in a mouse model of fibromyalgia. Molecular pain 66 24957987
2012 Simultaneous disruption of mouse ASIC1a, ASIC2 and ASIC3 genes enhances cutaneous mechanosensitivity. PloS one 64 22506072
2011 Selective targeting of ASIC3 using artificial miRNAs inhibits primary and secondary hyperalgesia after muscle inflammation. Pain 64 21843914
2013 Sea anemone peptide with uncommon β-hairpin structure inhibits acid-sensing ion channel 3 (ASIC3) and reveals analgesic activity. The Journal of biological chemistry 63 23801332
2010 Femoral artery occlusion increases expression of ASIC3 in dorsal root ganglion neurons. American journal of physiology. Heart and circulatory physiology 59 20852050
2004 Characterisation of DRASIC in the mouse inner ear. Hearing research 58 15051137
2013 pH-evoked dural afferent signaling is mediated by ASIC3 and is sensitized by mast cell mediators. Headache 57 23808707
2017 TDAG8, TRPV1, and ASIC3 involved in establishing hyperalgesic priming in experimental rheumatoid arthritis. Scientific reports 56 28827659
2012 Acid-sensing ion channels (ASICs) in mouse skeletal muscle afferents are heteromers composed of ASIC1a, ASIC2, and ASIC3 subunits. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 52 23109675
2007 Deletion of the acid-sensing ion channel ASIC3 prevents gastritis-induced acid hyperresponsiveness of the stomach-brainstem axis. Pain 51 17531389
2010 Mice lacking Asic3 show reduced anxiety-like behavior on the elevated plus maze and reduced aggression. Genes, brain, and behavior 50 20497234
2018 The ASIC3/P2X3 cognate receptor is a pain-relevant and ligand-gated cationic channel. Nature communications 47 29636447
2017 Identification of a cono-RFamide from the venom of Conus textile that targets ASIC3 and enhances muscle pain. Proceedings of the National Academy of Sciences of the United States of America 46 28396446
2001 ASIC3: a lactic acid sensor for cardiac pain. TheScientificWorldJournal 46 12805843
2017 Glioblastoma cancer stem cell lines express functional acid sensing ion channels ASIC1a and ASIC3. Scientific reports 45 29057936
2002 The co-expression of ASIC3 with calcitonin gene-related peptide and parvalbumin in the rat trigeminal ganglion. Brain research 44 12101052
2021 ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration. Aging 40 33824228
2008 Enhanced muscle fatigue occurs in male but not female ASIC3-/- mice. American journal of physiology. Regulatory, integrative and comparative physiology 39 18305024
2008 Effects of selective spinal nerve ligation on acetic acid-induced nociceptive responses and ASIC3 immunoreactivity in the rat dorsal root ganglion. Brain research 39 18534561
2016 Muscle IL1β Drives Ischemic Myalgia via ASIC3-Mediated Sensory Neuron Sensitization. The Journal of neuroscience : the official journal of the Society for Neuroscience 38 27358445
2015 Combined, but not individual, blockade of ASIC3, P2X, and EP4 receptors attenuates the exercise pressor reflex in rats with freely perfused hindlimb muscles. Journal of applied physiology (Bethesda, Md. : 1985) 38 26472871
2017 Targeting ASIC3 for Relieving Mice Fibromyalgia Pain: Roles of Electroacupuncture, Opioid, and Adenosine. Scientific reports 37 28440280
2021 Heartburn sensation in nonerosive reflux disease: pattern of superficial sensory nerves expressing TRPV1 and epithelial cells expressing ASIC3 receptors. American journal of physiology. Gastrointestinal and liver physiology 32 33655767
2008 SMAD3 functions as a transcriptional repressor of acid-sensing ion channel 3 (ASIC3) in nucleus pulposus cells of the intervertebral disc. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 32 18466073
2008 Increase of insulin sensitivity and reversal of age-dependent glucose intolerance with inhibition of ASIC3. Biochemical and biophysical research communications 32 18466760
2021 Inhibiting BDNF/TrkB.T1 receptor improves resiniferatoxin-induced postherpetic neuralgia through decreasing ASIC3 signaling in dorsal root ganglia. Journal of neuroinflammation 30 33874962
2010 Detection of acid-sensing ion channel 3 (ASIC3) in periodontal Ruffini endings of mouse incisors. Neuroscience letters 30 21078372
2015 The role of periodontal ASIC3 in orofacial pain induced by experimental tooth movement in rats. European journal of orthodontics 29 26675477
2017 Sensitization of ASIC3 by proteinase-activated receptor 2 signaling contributes to acidosis-induced nociception. Journal of neuroinflammation 28 28754162
2013 ASIC3 in the cerebrospinal fluid-contacting nucleus of brain parenchyma contributes to inflammatory pain in rats. Neurological research 28 24512021
2021 Metformin Attenuates Bone Cancer Pain by Reducing TRPV1 and ASIC3 Expression. Frontiers in pharmacology 26 34421611
2012 Responses of glomus cells to hypoxia and acidosis are uncoupled, reciprocal and linked to ASIC3 expression: selectivity of chemosensory transduction. The Journal of physiology 26 23165770
2015 Role of TRPV1 and ASIC3 channels in experimental occlusal interference-induced hyperalgesia in rat masseter muscle. European journal of pain (London, England) 24 26201614
2015 ASIC3 Mediates Itch Sensation in Response to Coincident Stimulation by Acid and Nonproton Ligand. Cell reports 24 26440887
2008 Acid-sensing ion channel 3 (ASIC3) cell surface expression is modulated by PSD-95 within lipid rafts. American journal of physiology. Cell physiology 24 18579798
2018 TRPM8, ASIC1, and ASIC3 localization and expression in the human oropharynx. Neurogastroenterology and motility 22 29971861
2016 Distinct roles of ASIC3 and TRPV1 receptors in electroacupuncture-induced segmental and systemic analgesia. Frontiers of medicine 22 27896621
2024 Sensory ASIC3 channel exacerbates psoriatic inflammation via a neurogenic pathway in female mice. Nature communications 20 38902277
2022 The ASIC3-M-CSF-M2 macrophage-positive feedback loop modulates fibroblast-to-myofibroblast differentiation in skin fibrosis pathogenesis. Cell death & disease 20 35661105
2006 ASIC3-immunoreactive neurons in the rat vagal and glossopharyngeal sensory ganglia. Brain research 20 16510130
2020 ASIC3 inhibition modulates inflammation-induced changes in the activity and sensitivity of Aδ and C fiber sensory neurons that innervate bone. Molecular pain 19 33280501
2019 Effects of hypoxia and ASIC3 on nucleus pulposus cells: From cell behavior to molecular mechanism. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 18 31202172
2020 c-Jun/p38MAPK/ASIC3 pathways specifically activated by nerve growth factor through TrkA are crucial for mechanical allodynia development. Pain 16 31977937
2019 ASIC3-dependent metabolomics profiling of serum and urine in a mouse model of fibromyalgia. Scientific reports 16 31431652
2014 Role of TRPV1 and ASIC3 in formalin-induced secondary allodynia and hyperalgesia. Pharmacological reports : PR 15 25443722
2019 Endogenous Neuropeptide Nocistatin Is a Direct Agonist of Acid-Sensing Ion Channels (ASIC1, ASIC2 and ASIC3). Biomolecules 14 31443477
2015 ASIC3 contributes to the blunted muscle metaboreflex in heart failure. Medicine and science in sports and exercise 14 24983337
2010 Acid-induced CGRP release from the stomach does not depend on TRPV1 or ASIC3. Neurogastroenterology and motility 13 20100279
2008 Genetic variation in the ASIC3 gene influences blood pressure levels in Taiwanese. Journal of hypertension 13 18854755
2021 Paradoxical Potentiation of Acid-Sensing Ion Channel 3 (ASIC3) by Amiloride via Multiple Mechanisms and Sites Within the Channel. Frontiers in physiology 12 34721074
2018 Expression of ASIC3 in the Trigeminal Nucleus Caudalis Plays a Role in a Rat Model of Recurrent Migraine. Journal of molecular neuroscience : MN 12 30209688
2012 Osthole, a herbal compound, alleviates nucleus pulposus-evoked nociceptive responses through the suppression of overexpression of acid-sensing ion channel 3 (ASIC3) in rat dorsal root ganglion. Medical science monitor : international medical journal of experimental and clinical research 12 22648244
2019 Effect of knockout of the ASIC3 on cardiovascular reflexes arising from hindlimb muscle in decerebrated rats. American journal of physiology. Regulatory, integrative and comparative physiology 11 31347922
2024 Fenofibrate ameliorates nitroglycerin-induced migraine in rats: Role of CGRP/p-CREB/P2X3 and NGF/PKC/ASIC3 signaling pathways. European journal of pharmacology 10 38795754
2022 Single Subcutaneous Injection of Lysophosphatidyl-Choline Evokes ASIC3-Dependent Increases of Spinal Dorsal Horn Neuron Activity. Frontiers in molecular neuroscience 10 35774865
2018 The Beneficial Effects of Electroacupuncture at PC6 Acupoints (Neiguan) on Myocardial Ischemia in ASIC3 -/- mice. Journal of acupuncture and meridian studies 10 29608997
2018 Up-regulation of ASIC3 expression by β-estradiol. Neuroscience letters 10 30114476
2014 Abnormal cardiac autonomic regulation in mice lacking ASIC3. BioMed research international 10 24804235
2024 ASIC3-activated key enzymes of de novo lipid synthesis supports lactate-driven EMT and the metastasis of colorectal cancer cells. Cell communication and signaling : CCS 9 39095886
2018 Role of ASIC3, Nav1.7 and Nav1.8 in electroacupuncture-induced analgesia in a mouse model of fibromyalgia pain. Acupuncture in medicine : journal of the British Medical Acupuncture Society 9 29343477
2015 Conversed mutagenesis of an inactive peptide to ASIC3 inhibitor for active sites determination. Toxicon : official journal of the International Society on Toxinology 9 26686983
2010 Association between an ASIC3 gene variant and insulin resistance in Taiwanese. Clinica chimica acta; international journal of clinical chemistry 9 20416288
2023 Amiloride alleviates morphine tolerance by suppressing ASIC3-dependent neuroinflammation in the spinal cord. European journal of pharmacology 8 37918499
2006 Peripherally applied neuropeptide SF is equally algogenic in wild type and ASIC3-/- mice. Neuroscience research 8 16730827
2023 Vacuolar-ATPase-mediated muscle acidification caused muscular mechanical nociceptive hypersensitivity after chronic stress in rats, which involved extracellular matrix proteoglycan and ASIC3. Scientific reports 7 37604935
2024 Complex alterations in inflammatory pain and analgesic sensitivity in young and ageing female rats: involvement of ASIC3 and Nav1.8 in primary sensory neurons. Inflammation research : official journal of the European Histamine Research Society ... [et al.] 6 38483556
2023 Involvement of ASIC3 and Substance P in Therapeutic Ultrasound-Mediated Analgesia in Mouse Models of Fibromyalgia. The journal of pain 6 37054767
2023 ASIC3 plays a protective role in delayed-onset muscle soreness (DOMS) through muscle acid sensation during exercise. Frontiers in pain research (Lausanne, Switzerland) 6 37795390
2018 Author Correction: The ASIC3/P2X3 cognate receptor is a pain-relevant and ligand-gated cationic channel. Nature communications 6 30120221
2012 Coxsackievirus and adenovirus receptor (CAR) mediates trafficking of acid sensing ion channel 3 (ASIC3) via PSD-95. Biochemical and biophysical research communications 6 22809504
2024 Activation of ASIC3/ERK pathway by paeoniflorin improves intestinal fluid metabolism and visceral sensitivity in slow transit constipated rats. The Kaohsiung journal of medical sciences 5 38634140
2023 Multiscale molecular dynamics simulations predict arachidonic acid binding sites in human ASIC1a and ASIC3 transmembrane domains. The Journal of general physiology 5 36625864
2016 ASIC2a-dependent increase of ASIC3 surface expression enhances the sustained component of the currents. BMB reports 5 27241858
2014 The selective ASIC3 inhibitor APETx2 alleviates gastric mucosal lesion in the rat. Die Pharmazie 5 25073401
2020 Roles of Up-Regulated Expression of ASIC3 in Sex Difference of Acid-Induced Duodenal HCO3 - Responses. Current pharmaceutical design 3 32303171
2019 Hydrophobic Amines and Their Guanidine Analogues Modulate Activation and Desensitization of ASIC3. International journal of molecular sciences 3 30959896
2025 Muscle afferent ASIC3 upregulation mediates the exacerbated exercise pressor reflex in male rats following hindlimb ischemia-reperfusion. Physiological reports 2 40635407
2022 Age-dependent and modality-specific changes in the phenotypic markers Nav1.8, ASIC3, P2X3 and TRPM8 in male rat primary sensory neurons during healthy aging. Biogerontology 2 36478541
2010 Genomic structure, polymorphism and expression of ACCN1 and ACCN3 genes in the horse. Animal genetics 2 21070287
2022 ASIC3 knockout alters expression and activity of P2X3 in muscle afferent nerves of rat model of peripheral artery disease. FASEB bioAdvances 1 35520394

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