Affinage

ARID5B

AT-rich interactive domain-containing protein 5B · UniProt Q14865

Length
1188 aa
Mass
132.4 kDa
Annotated
2026-06-09
60 papers in source corpus 23 papers cited in narrative 22 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ARID5B is an AT-rich interaction domain (ARID) transcription factor that functions as a sequence-specific DNA-targeting subunit for chromatin-modifying complexes, coupling promoter recognition to histone demethylation and deacetylation to control cell differentiation and metabolism (PMID:21532585, PMID:10329386). Its ARID domain recognizes the core AATA(C/T) motif through major- and minor-groove contacts, with two flexible interhelical loops (L1, L2) mediating phosphate-backbone and AT base-pair contacts (PMID:10329386, PMID:17407261). Mechanistically, ARID5B associates with the PKA-activated H3K9me2 demethylase PHF2 and targets this complex to promoters to erase repressive methyl marks and activate transcription (PMID:21532585); this ARID5B–PHF2 module promotes chondrocyte differentiation through Sox9 target genes (PMID:24276541) and activates SORBS2 to suppress epithelial-to-mesenchymal transition (PMID:37948999). In an opposing capacity, ARID5B assembles a chromatin repressor complex with MIER1, C16ORF87, HDAC1 and HDAC2, tethering deacetylase activity to active distal regulatory elements and promoters to repress B-cell proliferation and signaling genes [PMID:bio_10.1101_2025.10.17.683040]. ARID5B is a central regulator of fuel selection and lipid storage: knockout mice show defective brown/white adipose lipid accumulation and resistance to diet-induced obesity (PMID:14651970, PMID:33757861), and muscle-specific loss reprograms substrate use through control of prostanoid (PGI2) biosynthesis, GLUT4/TBC1D1-dependent glucose handling, and mitochondrial pyruvate transport (PMID:29196500, PMID:33023658, PMID:36743919). In hematopoiesis, ARID5B governs Pre-B cell expansion and metabolism (PMID:37483604, PMID:35924577), and acts as a collaborating oncogenic factor by co-occupying genomic targets with the TAL1 complex and activating MYC in T-ALL (PMID:29326336). ARID5B's own expression is set by lineage transcription factors and enhancer variants, including Ikaros (PMID:30420689) and a MEF2C/RUNX1-bound enhancer harboring an ALL risk allele (PMID:35575404).

Mechanistic history

Synthesis pass · year-by-year structured walk · 21 steps
  1. 1999 High

    Established the DNA recognition specificity of the ARID5B ARID domain, defining how this factor selects genomic targets.

    Evidence Binding interference, site-selection, and kinetic assays with base-analogue oligonucleotides

    PMID:10329386

    Open questions at the time
    • Does not identify in vivo target genes
    • Affinity determinants do not explain promoter selectivity in chromatin context
  2. 2001 High

    Showed that protein dynamics, not just static structure, drive ARID-domain DNA binding, resolving how flexible loops engage the duplex.

    Evidence 15N NMR relaxation and chemical shift perturbation mapping of the isolated ARID domain

    PMID:11478881

    Open questions at the time
    • No bound complex structure in this study
    • Full-length protein dynamics not addressed
  3. 2003 High

    Defined the first organismal role for ARID5B, establishing it as essential for postnatal lipid accumulation and adiposity.

    Evidence Mrf2/Arid5b knockout mouse phenotyping with high-fat diet challenge

    PMID:14651970

    Open questions at the time
    • Does not identify the transcriptional targets controlling lipid storage
    • Tissue of action not resolved
  4. 2007 High

    Provided the structural basis of ARID5B–DNA recognition, mapping which loops and helices contact AT base pairs.

    Evidence NMR structure of the ARID domain–DNA complex using paramagnetic spin-label constraints and docking

    PMID:17407261

    Open questions at the time
    • Isolated domain only; no full-length or co-factor-bound structure
    • Does not link recognition to transcriptional output
  5. 2011 High

    Revealed the core enzymatic mechanism by which ARID5B activates transcription—recruiting PKA-activated PHF2 to demethylate H3K9me2 at promoters.

    Evidence Co-IP, in vitro demethylase assays, ChIP, and PKA phosphorylation/mutagenesis

    PMID:21532585

    Open questions at the time
    • Promoter targeting specificity in vivo not defined
    • Does not address ARID5B-only or repressive functions
  6. 2013 High

    Connected the ARID5B–PHF2 demethylase module to a developmental program by showing it cooperates with Sox9 to drive chondrogenesis.

    Evidence Reciprocal Co-IP with Sox9, ChIP for H3K9me2, Arid5b-/- mouse phenotyping, and PHF2 knockdown rescue

    PMID:24276541

    Open questions at the time
    • Direct ARID5B binding to Sox9-target promoters not mapped genome-wide
    • Whether Sox9 recruits ARID5B or vice versa unresolved
  7. 2018 High

    Identified ARID5B as a collaborating oncogenic transcription factor in T-ALL that partners genomically with TAL1 and activates MYC.

    Evidence ChIP-seq co-occupancy with TAL1, knockdown/overexpression, and zebrafish thymocyte tumor model

    PMID:29326336

    Open questions at the time
    • Whether ARID5B uses its demethylase or repressor partners in this context unknown
    • Mechanism of MYC activation not resolved
  8. 2018 Medium

    Linked ARID5B expression control to the Ikaros tumor suppressor and confirmed its PHF2 interaction in ALL cells.

    Evidence Co-IP, CK2 inhibition, and ChIP for H3K4me3 at the ARID5B promoter

    PMID:30420689

    Open questions at the time
    • Two orthogonal methods from a single lab
    • Functional consequence of Ikaros-driven ARID5B levels not tested
  9. 2018 High

    Extended ARID5B function to immune-cell metabolism, showing a hypomethylation-induced short isoform drives oxidative metabolism and effector function in adaptive NK cells.

    Evidence siRNA knockdown/overexpression in human NK cells with mitochondrial, respiration, and IFN-γ readouts plus bisulfite sequencing

    PMID:30061358

    Open questions at the time
    • Transcriptional targets driving the metabolic phenotype not identified
    • Isoform-specific mechanism not resolved
  10. 2018 High

    Placed ARID5B upstream of prostanoid biosynthesis in muscle differentiation by linking it to COX-1/PGI synthase and PGI2.

    Evidence Arid5b-/- primary muscle cells, microarray, PGI2 ELISA, and iloprost rescue of myogenesis defects

    PMID:29196500

    Open questions at the time
    • Whether ARID5B directly binds Ptgs1/Ptgis promoters not shown
    • Direct vs indirect regulation unresolved
  11. 2020 Medium

    Defined a glucose-handling axis whereby ARID5B restrains GLUT4 surface localization via TBC1D1 in muscle.

    Evidence Arid5b-/- muscle glucose uptake/glycogen assays and GLUT4 immunofluorescence

    PMID:33023658

    Open questions at the time
    • Direct transcriptional control of TBC1D1 not demonstrated
    • Single-lab metabolic study
  12. 2021 Medium

    Showed ARID5B acts in a depot-specific manner in adipocytes, controlling subcutaneous WAT/liver lipid storage and inflammatory gene programs.

    Evidence Adipocyte-specific Fabp4-Cre Arid5b knockout with HFD challenge, triglyceride, macrophage staining, and RNA-seq

    PMID:33757861

    Open questions at the time
    • Mechanism of depot selectivity unknown
    • Direct inflammatory-gene targets not validated
  13. 2022 High

    Explained how an inherited ALL risk variant acts mechanistically, by disrupting a MEF2C/RUNX1 enhancer that controls ARID5B expression.

    Evidence dCas9-KRAB enhancer screen, ChIP, MEF2C-RUNX1 Co-IP, ATAC-seq, and UK Biobank analysis

    PMID:35575404

    Open questions at the time
    • Downstream leukemogenic consequence of altered ARID5B dosage not directly tested here
    • Other enhancers' contributions unquantified
  14. 2023 Medium

    Broadened the demethylase activity of ARID5B–PHF2 to H3K36me2 and tumor suppression, activating SORBS2 to block EMT in ovarian cancer.

    Evidence ChIP for H3K36me2 at SORBS2, ARID5B OE/KD, Co-IP, and EMT functional assays

    PMID:37948999

    Open questions at the time
    • Single-lab study with two orthogonal methods
    • How substrate specificity switches between H3K9me2 and H3K36me2 unresolved
  15. 2023 Medium

    Established ARID5B as a regulator of Pre-B cell expansion and metabolism, with dosage controlling B-cell output bidirectionally.

    Evidence Conditional knockout and ex vivo inhibition plus Vav1-driven overexpression, flow cytometry, BCR signaling, and metabolic/OCR assays

    PMID:35924577 PMID:37483604

    Open questions at the time
    • Direct transcriptional targets in B cells not defined
    • Link between metabolic and proliferative phenotypes mechanistically unresolved
  16. 2023 Medium

    Detailed how muscle ARID5B sets systemic fuel selection, controlling pyruvate oxidation and glucose partitioning with effects on adipose and liver.

    Evidence Skeletal-muscle-specific Arid5b knockout with metabolic phenotyping and gene expression

    PMID:36743919

    Open questions at the time
    • Direct targets (e.g., MPC) not validated as ARID5B-bound
    • Inter-organ signaling mediator unknown
  17. 2024 Low

    Defined ARID5B isoform architecture, identifying short/long isoforms with distinct BAH-like chromatin domains, independent promoters, and prognostic relevance in B-ALL.

    Evidence RNA-seq splice analysis, luciferase promoter assays, and clinical survival correlation

    PMID:38382358

    Open questions at the time
    • Functional consequence of the isoform difference is correlative, not mechanistic
    • Chromatin role of the BAH-like domain not tested directly
  18. 2024 Medium

    Revealed an inflammatory regulatory role in which the ARID5B long isoform, suppressed by TNF-α, dampens IL-6 and directly activates LINC01128 in synovial fibroblasts.

    Evidence siRNA/lentiviral isoform manipulation, luciferase, RIP, RNA pulldown, Co-IP, and ChIP for promoter binding

    PMID:38224186 PMID:38747454

    Open questions at the time
    • Mechanism by which the long isoform suppresses IL-6 not fully defined
    • Role of LINC01128 downstream unresolved
  19. 2025 Medium

    Identified ARID5B as the DNA-targeting subunit of a MIER1/HDAC1/HDAC2 repressor complex, providing a deacetylase-based repressive mechanism distinct from its demethylase activity.

    Evidence Mass spectrometry proteomics, CUT&RUN for genome-wide binding, and RNA-seq in B-ALL cells (preprint)

    PMID:bio_10.1101_2025.10.17.683040

    Open questions at the time
    • Preprint, not yet peer-reviewed
    • How ARID5B switches between activating and repressive complexes unknown
  20. 2025 Medium

    Connected ARID5B to neuronal excitability, showing WDR5-driven ARID5B upregulation represses GABA-A receptor subunits to promote epileptogenesis.

    Evidence ChIP-seq for H3K4me3, RNA-seq, WDR5 knockdown/neuronal knockout, patch-clamp, and video-EEG

    PMID:41510154

    Open questions at the time
    • Whether ARID5B binds GABAAR subunit promoters directly not shown
    • Which ARID5B partner complex mediates repression here unknown
  21. 2025 Low

    Placed ARID5B in hepatic coagulation control as a downstream effector of ApoM regulating prothrombin secretion.

    Evidence RNA-seq target identification, Arid5b knockdown in HepG2, ApoM mouse models, and prothrombin ELISA

    PMID:40719152

    Open questions at the time
    • Single knockdown with limited mechanistic depth
    • Direct transcriptional targets in hepatocytes not identified

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unknown what governs the switch between ARID5B's activating (PHF2 demethylase) and repressive (MIER1/HDAC) complexes, and how isoform identity and the BAH-like domain dictate which complex assembles at a given locus.
  • No structural model of full-length ARID5B with either partner complex
  • Isoform-specific complex assembly not directly tested
  • Determinants of context-dependent activation vs repression unresolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 5 GO:0140110 transcription regulator activity 4 GO:0060090 molecular adaptor activity 3
Localization
GO:0005634 nucleus 3
Pathway
R-HSA-1430728 Metabolism 4 R-HSA-1266738 Developmental Biology 3 R-HSA-1643685 Disease 3 R-HSA-4839726 Chromatin organization 3 R-HSA-74160 Gene expression (Transcription) 3
Partners
C16ORF87HDAC1HDAC2MIER1PHF2SOX9TAL1
Complex memberships
ARID5B-MIER1-HDAC1-HDAC2 repressor complexARID5B-PHF2 demethylase complex

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2011 ARID5B forms a histone demethylase complex with PHF2. PHF2 is enzymatically inactive alone but becomes an active H3K9Me2 demethylase upon PKA-mediated phosphorylation; phosphorylated PHF2 then associates with ARID5B (a DNA-binding protein), and ARID5B itself is demethylated. The PHF2-ARID5B complex is then targeted to promoters where it removes the repressive H3K9Me2 mark to activate transcription. Co-immunoprecipitation, in vitro demethylase assays, ChIP, PKA phosphorylation assays, mutagenesis Nature cell biology High 21532585
2013 ARID5B physically associates with the transcription factor Sox9 and recruits the histone demethylase PHF2 to the promoter regions of Sox9 target genes, stimulating H3K9me2 demethylation and thereby promoting chondrocyte differentiation. Arid5b-/- mice show retarded growth and delayed endochondral ossification, and H3K9me2 levels are increased at chondrogenic marker gene promoters in Arid5b-deficient chondrocytes. Co-immunoprecipitation (physical association with Sox9), ChIP (H3K9me2 levels at promoters), Arid5b-/- mouse phenotyping, PHF2 knockdown rescue experiments Nature communications High 24276541
1999 The ARID (MRF2/ARID5B) DNA-binding domain recognizes the core sequence AATA(C/T) through contacts in both major and minor grooves. Major groove contacts at positions 2, 3, and 4 of the core sequence are required for high-affinity binding, while positions 1 and 5 are contacted through the minor groove. The core sequence alone is not sufficient for high-affinity binding. Binding interference assays, binding site selection assays, kinetic analyses with synthetic oligonucleotides containing single base analogues Biochemical and biophysical research communications High 10329386
2001 NMR backbone dynamics of the MRF2/ARID5B ARID domain show that two flexible interhelical loops (and the C-terminal tail) are involved in DNA recognition. Upon DNA binding, flexible loops show reduced mobility while some well-structured regions (including the putative DNA-contacting helix) show decreased order parameters, indicating that protein dynamics are integral to DNA binding. 15N NMR relaxation measurements, model-free analysis, chemical shift perturbation mapping Biochemistry High 11478881
2007 The three-dimensional structure of the MRF2/ARID5B ARID domain in complex with target DNA was determined by NMR using paramagnetic spin-label distance constraints. MRF2 contacts DNA mainly through two flexible loops (L1 and L2): L1 contacts the phosphate backbone, while L2 and residues in adjacent helices interact with AT base pairs in the major groove. NMR spectroscopy with paramagnetic spin labeling, docking calculations Biochemistry High 17407261
2003 Targeted disruption of Mrf2/Arid5b in mice results in high neonatal mortality, severely reduced lipid accumulation in brown adipose tissue in neonates, and lean adult phenotype with significant reductions in brown and white adipose tissues. Mrf2-/- mice are resistant to high-fat diet-induced obesity, demonstrating that ARID5B is essential for lipid accumulation in postnatal life. Mrf2-/- knockout mouse generation and phenotyping (body composition, adipose tissue histology, high-fat diet challenge) Biochemical and biophysical research communications High 14651970
2018 A short isoform of ARID5B is selectively induced through DNA hypomethylation in adaptive (cytomegalovirus-expanded) NK cells. Knockdown and overexpression studies demonstrated that ARID5B directly promotes mitochondrial membrane potential, expression of genes encoding electron transport chain components, oxidative metabolism, survival, and IFN-γ production in these cells. ARID5B knockdown (siRNA) and overexpression in human NK cells; measurement of mitochondrial membrane potential, oxygen consumption rate, and IFN-γ production; bisulfite sequencing for DNA methylation The Journal of experimental medicine High 30061358
2018 ARID5B is a direct transcriptional target of the TAL1 oncogenic complex in T-ALL and functions as a collaborating oncogenic factor. ARID5B co-occupies target genes with TAL1 and coordinately controls their expression. ARID5B positively regulates expression of TAL1 and its regulatory partners, and activates MYC expression. Forced expression of ARID5B in immature thymocytes causes thymus retention, differentiation arrest, radioresistance, and tumor formation in zebrafish. ChIP-seq (ARID5B and TAL1 co-occupancy), knockdown/overexpression studies, ARID5B forced expression in zebrafish thymocytes, superenhancer analysis Genes & development High 29326336
2018 ARID5B physically interacts with PHF2 in acute lymphoblastic leukemia cells. Ikaros directly regulates ARID5B expression, and restoring Ikaros function via Casein Kinase II inhibition promotes ARID5B expression through recruitment of H3K4me3 at the ARID5B promoter. Co-immunoprecipitation (ARID5B-PHF2 interaction), CK2 inhibition with CK2i, ChIP (H3K4me3 at ARID5B promoter), gene expression analysis Oncogenesis Medium 30420689
2018 Arid5b-/- primary skeletal muscle cells exhibit differentiation defects and impaired sarcomeric assembly. Mechanistically, Arid5b-/- cells show down-regulation of COX-1 (Ptgs1) and PGI synthase (Ptgis), leading to reduced PGI2 production. Treatment with the PGI2 analog iloprost rescues defects in myotube formation, migration, and fusion, placing ARID5B upstream of the prostanoid biosynthesis pathway in myogenesis. Primary cell isolation from Arid5b-/- mice, microarray, RT-PCR, Western blot, ELISA (PGI2), Boyden chamber migration assay, iloprost rescue experiment FASEB journal High 29196500
2020 Arid5b knockout in skeletal muscle leads to increased basal glucose uptake, glycogen content, glucose oxidation, and ATP production. The mechanistic basis involves downregulation of TBC1D1 (a negative regulator of GLUT4 translocation), resulting in increased GLUT4 localization at the plasma membrane in Arid5b-/- muscle. Arid5b-/- mouse skeletal muscle analysis, glucose uptake assays, glycogen measurements, coimmunofluorescence for GLUT4/dystrophin, protein expression analysis Biological research Medium 33023658
2021 Fabp4-Cre conditional Arid5b knockout mice (adipocyte-specific) are resistant to high-fat diet-induced weight gain, with decreased lipid accumulation specifically in subcutaneous (inguinal) white adipose tissue and liver, but not in gonadal WAT. RNA-seq revealed decreased expression of inflammation-associated genes in IWAT adipocytes of FSKO mice, suggesting ARID5B regulates inflammatory signaling from specific WAT depots to the liver. Conditional Fabp4-Cre; Arid5bFLOX/FLOX mouse generation, HFD challenge, tissue weight, triglyceride measurements, CD68 staining for macrophages, RNA-seq Molecular and cellular endocrinology Medium 33757861
2022 ARID5B risk variants at the ALL susceptibility locus function through cis-regulatory elements. CRISPR-based enhancer screening identified six cis-regulatory elements at the ARID5B locus. The top ALL risk variant (rs7090445) lies within the strongest enhancer, which is distally tethered to the ARID5B promoter. The risk allele disrupts the MEF2C binding motif, reducing MEF2C affinity and decreasing local chromatin accessibility. MEF2C influences ARID5B expression in ALL, likely via a transcription factor complex with RUNX1. dCas9-KRAB enhancer interference screening, chromatin immunoprecipitation (ChIP), coimmunoprecipitation (MEF2C-RUNX1 complex), ATAC-seq (chromatin accessibility), targeted sequencing, UK Biobank genetic analysis Journal of the National Cancer Institute High 35575404
2023 ARID5B-PHF2 complex promotes histone demethylation at H3K36me2 at the SORBS2 promoter, thereby activating SORBS2 transcription and suppressing epithelial-to-mesenchymal transition (EMT) and tumor generation in ovarian cancer cells. ChIP (H3K36me2 at SORBS2 promoter), ARID5B overexpression/knockdown, Co-immunoprecipitation, in vivo and in vitro functional assays for EMT Pathology, research and practice Medium 37948999
2023 ARID5B regulates B cell development at the Pre-B cell stage: Arid5b deletion in vivo and ex vivo causes increased large and small Pre-B cell proportions with enhanced proliferation, and enhanced fatty acid uptake and oxidation at the Pre-B stage. ARID5B expression is upregulated at the Pre-B stage and maintained through later B cell development. In vivo Arid5b conditional knockout and ex vivo Arid5b inhibition; flow cytometry for B cell fractions; metabolic assays (fatty acid uptake, oxidation); gene expression analysis in mouse and human bone marrow Frontiers in immunology Medium 37483604
2023 Arid5b overexpression in mice (Vav1-driven transgenic) causes a dramatic reduction in circulating B cells and B cell fractions in peripheral blood, bone marrow, and spleen. ARID5B overexpression leads to defects in B cell activation in vitro with hyperactivation of B-cell receptor signaling at baseline, and increases mitochondrial oxygen consumption rate in naïve and stimulated B cells. Vav1-Cre transgenic mouse overexpression, flow cytometry, in vitro B cell activation assays, mitochondrial oxygen consumption rate measurement Haematologica Medium 35924577
2023 Muscle-specific deletion of Arid5b leads to preferential utilization of fatty acids for energy generation in skeletal muscle, decreased adipose tissue weight (via increased phospho-HSL/HSL in WAT), and glucose diversion into the pentose phosphate pathway and glycolysis for lactate export. Glucose oxidation is reduced in conjunction with downregulation of the mitochondrial pyruvate carrier (MPC). This establishes ARID5B as a regulator of fuel selection in skeletal muscle that systemically influences adipose and liver metabolism. Skeletal muscle-specific Arid5b knockout (Arid5b MKO) mice, metabolic phenotyping, gene expression analysis, tissue weight measurements Frontiers in endocrinology Medium 36743919
2024 ARID5B directly activates LINC01128 transcription by binding to its promoter (confirmed by ChIP). The long isoform of ARID5B is negatively regulated by TNF-α in rheumatoid arthritis synovial fibroblasts, and suppresses IL-6 production stimulated by TNF-α. siRNA knockdown and lentiviral overexpression of ARID5B isoforms confirmed the long isoform as a negative modulator of IL-6. siRNA knockdown, lentiviral overexpression, luciferase reporter assay, RNA immunoprecipitation, RNA pulldown, co-immunoprecipitation, ChIP Clinical and translational medicine / Immunological medicine Medium 38224186 38747454
2025 ARID5B assembles into a chromatin repressor complex with MIER1, C16ORF87, HDAC1, and HDAC2 in B-ALL cells. CUT&RUN mapping showed ARID5B binds active regions of the genome and tethers HDAC1/HDAC2 to distal regulatory elements and promoters. Genes actively repressed by this ARID5B complex are involved in B cell proliferation and B cell-specific signaling. Proteomics (mass spectrometry for complex identification), CUT&RUN (ARID5B, HDAC1, HDAC2 genome-wide binding), RNA-seq (transcriptomic effects) bioRxivpreprint Medium bio_10.1101_2025.10.17.683040
2025 In epileptogenesis, WDR5 enhances H3K4me3 deposition at the Arid5b promoter, driving transcriptional upregulation of ARID5B in hippocampal neurons. The upregulated ARID5B subsequently represses GABA-A receptor (GABAAR) subunit expression, impairing inhibitory synaptic transmission and facilitating epileptogenesis. ChIP-seq (H3K4me3 at Arid5b promoter), RNA-seq, WDR5 knockdown, neuron-specific WDR5 knockout, whole-cell patch-clamp recordings, video-EEG monitoring Theranostics Medium 41510154
2025 ApoM upregulates Arid5b expression in hepatocytes, and Arid5b knockdown increases culture-medium prothrombin levels while decreasing cellular prothrombin levels, reversing ApoM's inhibitory effect on prothrombin secretion. This places ARID5B downstream of ApoM in regulation of hepatic prothrombin secretion, independent of S1P receptors. RNA-seq (Arid5b identification), Arid5b knockdown in HepG2 cells, ApoM overexpression/knockout mouse models, prothrombin ELISA Thrombosis and haemostasis Low 40719152
2024 Two transcriptionally distinct ARID5B isoforms (short and long) differ in an encoded BAH-like chromatin interaction domain. Both isoforms have functionally independent proximal promoters as shown by luciferase reporter assays. Increased short isoform expression is associated with decreased event-free and overall survival in B-ALL, and the isoform ratio strongly correlates with B-ALL prognostic stratification. RNA-seq splice junction analysis, luciferase reporter assays for independent promoter activity, correlation with clinical survival outcomes Biochemical and biophysical research communications Low 38382358

Source papers

Stage 0 corpus · 60 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2012 Meta-analysis followed by replication identifies loci in or near CDKN1B, TET3, CD80, DRAM1, and ARID5B as associated with systemic lupus erythematosus in Asians. American journal of human genetics 175 23273568
2011 PKA-dependent regulation of the histone lysine demethylase complex PHF2-ARID5B. Nature cell biology 154 21532585
2018 ARID5B regulates metabolic programming in human adaptive NK cells. The Journal of experimental medicine 107 30061358
2013 Arid5b facilitates chondrogenesis by recruiting the histone demethylase Phf2 to Sox9-regulated genes. Nature communications 80 24276541
2010 Replication analysis confirms the association of ARID5B with childhood B-cell acute lymphoblastic leukemia. Haematologica 64 20460642
2018 ARID5B as a critical downstream target of the TAL1 complex that activates the oncogenic transcriptional program and promotes T-cell leukemogenesis. Genes & development 55 29326336
1999 The novel Mrf-2 DNA-binding domain recognizes a five-base core sequence through major and minor-groove contacts. Biochemical and biophysical research communications 52 10329386
2011 Role of 657del5 NBN mutation and 7p12.2 (IKZF1), 9p21 (CDKN2A), 10q21.2 (ARID5B) and 14q11.2 (CEBPE) variation and risk of childhood ALL in the Polish population. Leukemia research 49 21889209
2003 Neonatal mortality and leanness in mice lacking the ARID transcription factor Mrf-2. Biochemical and biophysical research communications 49 14651970
2013 Association of three polymorphisms in ARID5B, IKZF1 and CEBPE with the risk of childhood acute lymphoblastic leukemia in a Chinese population. Gene 47 23608171
2013 Genetic variants in ARID5B and CEBPE are childhood ALL susceptibility loci in Hispanics. Cancer causes & control : CCC 41 23836053
2013 High-resolution melting analyses for genetic variants in ARID5B and IKZF1 with childhood acute lymphoblastic leukemia susceptibility loci in Taiwan. Blood cells, molecules & diseases 33 24200646
2001 Dynamics of the Mrf-2 DNA-binding domain free and in complex with DNA. Biochemistry 33 11478881
2020 The Role of ARID5B in Acute Lymphoblastic Leukemia and Beyond. Frontiers in genetics 30 32595701
2014 Associations of novel genetic variations in the folate-related and ARID5B genes with the pharmacokinetics and toxicity of high-dose methotrexate in paediatric acute lymphoblastic leukaemia. British journal of haematology 30 24712521
2007 Determination of the three-dimensional structure of the Mrf2-DNA complex using paramagnetic spin labeling. Biochemistry 30 17407261
2014 Confirmation of childhood acute lymphoblastic leukemia variants, ARID5B and IKZF1, and interaction with parental environmental exposures. PloS one 29 25310577
2018 Aberrant ARID5B expression and its association with Ikaros dysfunction in acute lymphoblastic leukemia. Oncogenesis 25 30420689
2022 Molecular Mechanisms of ARID5B-Mediated Genetic Susceptibility to Acute Lymphoblastic Leukemia. Journal of the National Cancer Institute 21 35575404
2013 ARID5B gene rs10821936 polymorphism is associated with childhood acute lymphoblastic leukemia: a meta-analysis based on 39,116 subjects. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 21 23975371
2013 Intron 3 of the ARID5B gene: a hot spot for acute lymphoblastic leukemia susceptibility. Journal of cancer research and clinical oncology 19 24013273
2017 Association of ARID5B gene variants with acute lymphoblastic leukemia in Yemeni children. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 17 28381164
2012 Associations of variations in the MRF2/ARID5B gene with susceptibility to type 2 diabetes in the Japanese population. Journal of human genetics 16 22971728
2019 Novel phenotypes observed in patients with ETV6-linked leukaemia/familial thrombocytopenia syndrome and a biallelic ARID5B risk allele as leukaemogenic cofactor. Journal of medical genetics 14 31704777
2018 Reduced prostaglandin I2 signaling in Arid5b-/- primary skeletal muscle cells attenuates myogenesis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 14 29196500
2020 Increased glucose metabolism in Arid5b-/- skeletal muscle is associated with the down-regulation of TBC1 domain family member 1 (TBC1D1). Biological research 12 33023658
2019 Variants in ARID5B gene are associated with the development of acute lymphoblastic leukemia in Mexican children. Annals of hematology 12 31227872
2023 ARID5B promoted the histone demethylation of SORBS2 and hampered the metastasis of ovarian cancer. Pathology, research and practice 10 37948999
2021 Mice with Fabp4-Cre ablation of Arid5b are resistant to diet-induced obesity and hepatic steatosis. Molecular and cellular endocrinology 10 33757861
2019 ARID5B gene polymorphisms and the risk of childhood acute lymphoblastic leukemia: a meta-analysis. International journal of hematology 10 31111395
2023 ARID5B influences B-cell development and function in mouse. Haematologica 9 35924577
2024 ARID5B-mediated LINC01128 epigenetically activated pyroptosis and apoptosis by promoting the formation of the BTF3/STAT3 complex in β2GPI/anti-β2GPI-treated monocytes. Clinical and translational medicine 8 38224186
2021 Transporters, TBC1D4, and ARID5B Variants to Explain Glycated Hemoglobin Variability in Patients with Type 2 Diabetes. Pharmacology 8 34265779
2008 Genetic variations of Mrf-2/ARID5B confer risk of coronary atherosclerosis in the Japanese population. International heart journal 8 18612189
2019 Association of ARID5B and IKZF1 Variants with Leukemia from Northern India. Genetic testing and molecular biomarkers 7 30810385
2019 Association of genes ARID5B, CEBPE and folate pathway with acute lymphoblastic leukemia in a population from the Brazilian Amazon region. Leukemia research reports 7 31867206
2022 Impact of Variants in the ATIC and ARID5B Genes on Therapeutic Failure with Imatinib in Patients with Chronic Myeloid Leukemia. Genes 6 35205374
2019 ARID5B rs10821936 and rs10994982 gene polymorphisms and acute lymphoblastic leukemia: relation to disease susceptibility and outcome. Pediatric hematology and oncology 6 31424309
2023 Muscle-specific deletion of Arid5b causes metabolic changes in skeletal muscle that affect adipose tissue and liver. Frontiers in endocrinology 5 36743919
2020 Association of relapse-linked ARID5B single nucleotide polymorphisms with drug resistance in B-cell precursor acute lymphoblastic leukemia cell lines. Cancer cell international 5 33499894
2018 Association of ARID5B Genetic Variants with Risk of Childhood B Cell Precursor Acute Lymphoblastic Leukaemia in Latvia. Asian Pacific journal of cancer prevention : APJCP 5 29373897
2017 Molecular studies reveal MLL-MLLT10/AF10 and ARID5B-MLL gene fusions displaced in a case of infantile acute lymphoblastic leukemia with complex karyotype. Oncology letters 5 28781666
2021 ARID5B rs10821936 and rs10994982 gene polymorphism and susceptibility to juvenile systemic lupus erythematosus and lupus nephritis. Lupus 4 33888010
2021 Genetic association of ARID5B with the risk of colorectal cancer within Jammu and Kashmir, India. Genes & genetic systems 4 34803080
2024 ARID5B is a negative modulator of IL-6 production in rheumatoid arthritis synovial fibroblasts. Immunological medicine 3 38747454
2023 Contributions of ARID5B, IKZF1, PIP4K2A, and GATA3 Gene Polymorphisms to Childhood Acute Lymphoblastic Leukemia in a Chinese Population. Journal of pediatric hematology/oncology 3 36952466
2023 ARID5B regulates fatty acid metabolism and proliferation at the Pre-B cell stage during B cell development. Frontiers in immunology 3 37483604
2023 ARID5B, IKZF1, GATA3, CEBPE, and CDKN2A germline polymorphisms and predisposition to childhood acute lymphoblastic leukemia. Pediatric hematology and oncology 3 37578068
2022 Hsa-miR-422a Originated from Short Interspersed Nuclear Element Increases ARID5B Expression by Collaborating with NF-E2. Molecules and cells 3 35444070
2015 Rs4948496 within ARID5B gene is associated with clinical features of systemic lupus erythematosus in the Chinese Han population. The Journal of dermatology 3 25808444
2014 Differential expression of arid5b isoforms in Xenopus laevis pronephros. The International journal of developmental biology 3 25354457
2023 Association of two ARID5B gene variant single nucleotide polymorphisms with acute lymphoblastic leukemia in the Egyptian population. Asian Pacific journal of cancer prevention : APJCP 2 36708585
2022 Hsa_circ_0102485 inhibits the growth of cancer cells by regulating the miR-188-3p/ARID5B/AR axis in prostate carcinoma. Pathology, research and practice 2 35939970
2012 [Research advances on correlation of ARID5B gene with childhood acute lymphoblastic leukemia - review]. Zhongguo shi yan xue ye xue za zhi 1 23114164
2026 Neuron-specific WDR5 epigenetically upregulates ARID5B to impair GABAergic synaptic transmission and promotes epileptogenesis. Theranostics 0 41510154
2025 Hepatic Apolipoprotein M Suppresses Hepatocyte Secretion of Prothrombin by Upregulating Arid5B. Thrombosis and haemostasis 0 40719152
2025 [Correlation of ARID5B Gene Polymorphism and Risk of Childhood Acute Lymphoblastic Leukemia and Minimal Residual Disease]. Zhongguo shi yan xue ye xue za zhi 0 41234072
2024 Identification of an alternative short ARID5B isoform associated with B-ALL survival. Biochemical and biophysical research communications 0 38382358
2024 Constitutional and acquired genetic variants in ARID5B in pediatric B-cell precursor acute lymphoblastic leukemia. Genes, chromosomes & cancer 0 38738968
2023 [Correlation between ARID5B Gene SNP and MTX Resistance in Children with ALL]. Zhongguo shi yan xue ye xue za zhi 0 37096502

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