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Showing BIN1 — AMPH2 is a alias.

BIN1

Myc box-dependent-interacting protein 1 · UniProt O00499

Length
593 aa
Mass
64.7 kDa
Annotated
2026-06-09
100 papers in source corpus 55 papers cited in narrative 55 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

BIN1 (Amphiphysin II) is a multi-domain membrane-remodeling adaptor whose N-BAR domain senses and generates membrane curvature in an autoinhibited, phosphoinositide-regulated manner (PMID:25350771, PMID:17059209). Its membrane-deforming activity is gated by an intramolecular interaction between its polybasic exon10 region and the SH3 domain, which is relieved by PI(4,5)P2 binding or by SH3 ligands such as PxxP-containing dynamin (PMID:15483625, PMID:25350771); cryo-EM shows the N-terminal amphipathic helix H0 nucleates cooperative N-BAR polymerization on membrane tubes (PMID:26487375). Through PI(4,5)P2 clustering BIN1 recruits and negatively regulates dynamin 2 GTPase activity, a relationship essential for T-tubule biogenesis: BIN1 scaffolds T-tubule membranes in skeletal and cardiac muscle, recruits Cav1.2 via microtubule-tethered delivery and drives N-WASP-dependent actin folding of T-tubule membranes for excitation-contraction coupling (PMID:25487648, PMID:33187981, PMID:20169111, PMID:24836577). Genetic loss of BIN1 causes perinatal cardiomyopathy in mice (PMID:12773571) and muscle-specific deletion recapitulates centronuclear myopathy that is rescued by lowering DNM2 (PMID:34371181, PMID:29130937), while disease-linked missplicing (myotonic dystrophy via MBNL1) and CNM coding mutations impair its membrane-tubulating activity (PMID:21623381, PMID:24755653). In the nucleus, BIN1 binds the MYC transactivation domain through its SH3 and MYC-binding domains to suppress MYC-driven transformation and to engage apoptotic and tumor-suppressive programs (PMID:8782822, PMID:10380878, PMID:15992821, PMID:22457068); it also binds and inhibits PARP1 and restrains E2F1/ATM-driven DNA repair to maintain cisplatin sensitivity (PMID:21447800, PMID:30733337). In neurons BIN1 controls presynaptic vesicle dynamics, synaptic transmission and AMPA receptor trafficking (PMID:32160554, PMID:30967682), and its SH3 domain directly binds the tau proline-rich domain in a phosphorylation- and conformation-regulated manner linking it to tauopathy and Alzheimer's disease (PMID:26395440, PMID:30487734, PMID:31065832).

Mechanistic history

Synthesis pass · year-by-year structured walk · 25 steps
  1. 1996 High

    Established BIN1's founding identity as a MYC-interacting tumor suppressor, defining a nuclear/oncogenic axis distinct from its later membrane roles.

    Evidence Interaction screen and growth-suppression assays against MYC-transformed cells

    PMID:8782822

    Open questions at the time
    • Did not define the binding domain or structural basis
    • Did not connect MYC suppression to membrane biology
  2. 1997 High

    Localized BIN1 to neuronal axon initial segments/nodes and muscle T-tubules, and identified it as a short-lived nuclear phosphoprotein mislocalized in tumors, foreshadowing dual nuclear and membrane functions.

    Evidence Immunofluorescence and subcellular fractionation in brain/muscle; pulse-chase and immunostaining in normal vs tumor cells

    PMID:9182667 PMID:9242458

    Open questions at the time
    • Did not establish the mechanism of T-tubule targeting
    • Functional consequence of tumor relocalization unresolved
  3. 1997 Medium

    Showed alternative splicing controls MYC-binding capacity and that MyoD drives muscle expression, introducing isoform switching as a core regulatory principle.

    Evidence Exon mapping, promoter reporter, cell-type RT-PCR

    PMID:9395479

    Open questions at the time
    • Functional impact of each splice variant not fully tested
    • Single-lab promoter analysis
  4. 1999 Medium

    Resolved that BIN1 suppresses growth by both MYC-dependent transactivation inhibition and MYC-independent mechanisms, and that brain exon 12A inclusion abolishes tumor-suppressor and apoptotic activity, linking splicing to oncogenic escape.

    Evidence Co-IP, transcriptional reporters, transformation and apoptosis assays with isoform comparison

    PMID:10380878 PMID:10449755

    Open questions at the time
    • MYC-independent effectors not identified
    • Mechanism by which exon 12A blocks function unclear at the time
  5. 2000 Medium

    Defined a caspase-independent, BAR-domain-dependent cell death program engaged by BIN1, distinguishing its apoptotic effect from canonical pathways.

    Evidence Domain mutagenesis and pharmacological inhibitors in cell death assays

    PMID:11032017 PMID:11306501

    Open questions at the time
    • Molecular executioners of this death program unknown
    • Single-lab characterization
  6. 2003 High

    Genetic knockout revealed that BIN1's essential in vivo role is cardiac, not endocytic, redirecting the field from a presumed generic endocytosis function toward muscle membrane organization.

    Evidence Constitutive Bin1 knockout mouse with histology, EM, and endocytosis/phagocytosis assays

    PMID:12773571

    Open questions at the time
    • Perinatal lethality limited adult analysis
    • Molecular cause of cardiomyopathy not yet defined
  7. 2004 High

    Identified the phosphoinositide-gated intramolecular exon10-SH3 mechanism, explaining how BIN1's SH3-ligand binding (including dynamin) is regulated by PI(4,5)P2.

    Evidence In vitro binding, PI(4,5)P2 titration, T-tubule formation assay

    PMID:15483625

    Open questions at the time
    • Did not resolve how this couples to membrane curvature generation
    • Single-lab biochemistry
  8. 2005 Medium

    Connected BIN1 loss to tumor immune escape via STAT1/NF-kB-driven IDO upregulation, broadening its tumor-suppressor role to immune evasion.

    Evidence Knockout mouse studies with pathway analysis in MMTV-Neu model

    PMID:15711557

    Open questions at the time
    • Direct molecular link between BIN1 and IDO transcription unresolved
    • Single-lab finding
  9. 2005 High

    Provided structural and biochemical definition of the BIN1 SH3-MYC interaction and showed phosphorylation of MYC Ser62 and intramolecular polyproline-SH3 binding disable it in tumor isoforms.

    Evidence NMR structure, SPR, biochemical binding

    PMID:15992821

    Open questions at the time
    • In vivo relevance of Ser62 regulation not tested
    • Dynamics of complex incompletely described
  10. 2006 High

    Crystal and interaction structures of the BAR domain and an SH3-MYC complex provided the architectural basis for membrane curvature and protein scaffolding.

    Evidence X-ray crystallography of BAR domain; yeast two-hybrid/Co-IP and infectivity for HCV NS5A binding

    PMID:16530520 PMID:17059209

    Open questions at the time
    • Convex-face protein partners not all identified
    • How curvature is dynamically tuned in cells unresolved
  11. 2009 High

    Linked BIN1 membrane tubulation to the cytoskeleton and endocytic recycling, showing BAR-domain binding to CLIP-170, cooperative tubulation with EHD1/RME-1, and SH3 scaffolding of sarcomere components.

    Evidence Co-IP, GST pulldown, RNAi, in vitro reconstitution with purified AMPH-1/RME-1, dominant-negative expression

    PMID:19004523 PMID:19633357 PMID:19915558

    Open questions at the time
    • Hierarchy among microtubule, actin and recycling functions unclear
    • Tissue specificity of each interaction not resolved
  12. 2009 Medium

    Placed BIN1 downstream of E2F1 as a transcriptional target mediating DNA-damage apoptosis independent of p53, integrating it into stress-response signaling.

    Evidence Promoter reporter, ChIP, siRNA, DNA damage apoptosis assays

    PMID:19629135

    Open questions at the time
    • Apoptotic effectors downstream of BIN1 unidentified
    • Single-lab study
  13. 2010 High

    Defined the cardiac mechanism by which BIN1 scaffolds T-tubules to deliver Cav1.2 via microtubule tethering, establishing its role in calcium handling.

    Evidence Immunogold EM, Co-IP, surface biotinylation, live-cell TIRF, shRNA knockdown

    PMID:20169111

    Open questions at the time
    • Did not address skeletal muscle EC coupling
    • Microtubule-membrane tether composition incompletely defined
  14. 2011 High

    Extended T-tubule scaffolding to skeletal muscle EC coupling and human heart failure, and identified MBNL1-dependent missplicing as a disease mechanism, plus a PARP1/MYC axis controlling cisplatin resistance.

    Evidence In vivo shRNA electroporation with electrophysiology; failing-heart tissue with multi-species knockdown; MBNL1 RNA-binding and transgenic mouse splicing model; PARP1 activity and binding assays

    PMID:21447800 PMID:21623381 PMID:21984944 PMID:22138472

    Open questions at the time
    • How missplicing produces functional loss structurally not fully defined
    • PARP1 nuclear and membrane roles not reconciled
  15. 2012 Medium

    Established direct BIN1-Tau interaction and modifier genetics, opening the neurodegeneration axis, alongside biophysical characterization of the disordered MYC-BIN1 complex.

    Evidence Co-IP, co-localization, Drosophila tau-toxicity suppression; NMR/SPR of MYC transactivation domain

    PMID:22457068 PMID:23399914

    Open questions at the time
    • Domain and residue-level basis of Tau binding not yet defined
    • Directionality of BIN1 effect on tau toxicity in mammals unclear
  16. 2013 High

    Mapped the BIN1 SH3-Tau proline-rich-domain interaction and its regulation by Tau Thr231 phosphorylation, defining a phospho-switch relevant to tauopathy.

    Evidence GST pulldown, NMR, primary neuron co-localization, phospho-specific analysis

    PMID:26395440

    Open questions at the time
    • Cellular consequence of disrupted SH3-PRD binding not yet measured
    • Link to pathological aggregation unresolved
  17. 2014 High

    Provided a comprehensive mechanistic and structural model of BIN1 membrane action: autoinhibition relieved by PI(4,5)P2, PI(4,5)P2 clustering to recruit dynamin, N-WASP-dependent T-tubule folding for electrical insulation, and CNM-mutant defects in tubulation.

    Evidence In vitro membrane deformation and dynamin recruitment assays, cardiac-specific KO with superresolution and electrophysiology, liposome tubulation of CNM mutants

    PMID:24755653 PMID:24836577 PMID:25332206 PMID:25350771 PMID:25487648 PMID:26487375

    Open questions at the time
    • Coupling between autoinhibition release and dynamin regulation in vivo not fully resolved
    • Distinct CNM mutant mechanisms not unified
  18. 2016 Medium

    Tied beta-adrenergic signaling and Rab8/EHBP1L1-dependent vesicular transport to BIN1, showing dynamic recruitment of phospho-RyR2 to dyads and apical trafficking roles.

    Evidence Co-IP, superresolution imaging, Bin1 heterozygous mice; binding assays and EHBP1L1-KO mouse in epithelia

    PMID:26733606 PMID:26833786

    Open questions at the time
    • Signaling input controlling BIN1 redistribution incompletely mapped
    • Generalizability of Rab8 complex beyond epithelia unclear
  19. 2017 High

    Demonstrated genetically that BIN1's key muscle function is negative regulation of DNM2, with Dnm2 reduction rescuing Bin1-null lethality, and characterized BAR-domain actin stabilization relevant to tau.

    Evidence Bin1-/- Dnm2+/- epistasis mice with in vitro GTPase assay; in vitro actin binding/bundling plus Drosophila tauopathy KD

    PMID:28893863 PMID:29130937

    Open questions at the time
    • Isoform-specific DNM2 regulation not fully resolved
    • Role of actin stabilization in disease unclear
  20. 2018 High

    Resolved the residue-level NMR structure of the BIN1 SH3-Tau interface and quantified how multisite Tau phosphorylation weakens binding and unmasks the intramolecular SH3-CLAP interaction.

    Evidence NMR, SPR, competition binding; BIN1 expression in hESC-cardiomyocytes for T-tubule induction

    PMID:30353632 PMID:30487734

    Open questions at the time
    • Physiological role of competition between Tau and CLAP unclear
    • In vivo consequence of phospho-weakening untested here
  21. 2019 High

    Defined neuronal and glial BIN1 functions: presynaptic vesicle dynamics and learning, calcineurin-controlled conformational gating of Tau binding, microglial control of Tau spreading, an LVGCC-Tau hyperexcitability axis, and the negative result that neuronal BIN1 does not regulate Abeta in vivo.

    Evidence Conditional neuronal/microglial KO with behavior, electrophysiology, EM, NMR conformational analysis, EV-Tau quantification, MEA recordings, and 5XFAD amyloid analysis

    PMID:30692199 PMID:30733337 PMID:31065832 PMID:31263146 PMID:32160554 PMID:32657270

    Open questions at the time
    • Sex-specific microglial effects not mechanistically explained
    • Reconciliation of pro- and anti-pathogenic neuronal roles incomplete
  22. 2020 Medium

    Expanded synaptic roles to postsynaptic AMPA receptor trafficking via Arf6/GluA1 complexes and clarified excitability and tau-background mortality phenotypes of neuronal Bin1 loss.

    Evidence Superresolution SIM, Co-IP, surface biotinylation, electrophysiology, conditional KO with transcriptomics, GTPase reconstitution assay

    PMID:30967682 PMID:31408457 PMID:33187981

    Open questions at the time
    • Direct structural basis of BIN1-Arf6-GluA1 complex undefined
    • Integration of pre- and postsynaptic functions unresolved
  23. 2021 High

    Validated BIN1-DNM2 as a therapeutic axis in centronuclear myopathy, identified Cavin4 as a T-tubule partner, defined endosome-based control of Abeta generation, and linked LOAD coding variants to impaired BACE1 recycling.

    Evidence Muscle-specific KO with DNM2 ASO rescue; Cavin4 KO in mouse and zebrafish; Co-IP of BIN1-BACE1 with variants and endosome/Abeta assays

    PMID:34371181 PMID:34375641 PMID:34633413

    Open questions at the time
    • Cardiac vs skeletal DNM2 isoform regulation differences unresolved
    • Mechanistic link between endosome enlargement and Abeta incompletely defined
  24. 2022 Medium

    Established isoform-specific neuronal control of early endosome size and BACE1/APP spatial separation (RIN3-dependent), and defined microglial BIN1 control of interferon and disease-associated activation programs.

    Evidence Drosophila and human neuron/organoid KO with isoform rescue, endosome morphometry, APP internalization and Abeta assays; microglial conditional KO with transcriptomics and cytokine measurement

    PMID:34998435 PMID:35241726 PMID:35526014

    Open questions at the time
    • Why isoform 1 but not isoform 9 controls endosome size mechanistically unclear
    • In vivo contribution of microglial interferon regulation to AD pathology unresolved
  25. 2023 Medium

    Resolved the balanced cardiac roles of BIN1, MTM1, and DNM2 in tuning T-tubule growth geometry, integrating phosphoinositide phosphatase and fission machinery with BIN1 isoform-specific tubulation.

    Evidence Developing cardiomyocytes, gene-modified HL-1 and hiPSC-CMs, Airyscan microscopy, Co-IP, Ca2+ recording

    PMID:37139790

    Open questions at the time
    • Quantitative stoichiometry balancing the three regulators in vivo undefined
    • Cardiac vs skeletal differences in MTM1 dependence unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How BIN1's distinct compartmentalized functions—nuclear MYC/PARP1/E2F1 tumor suppression, muscle T-tubule scaffolding, and neuronal endosomal/synaptic/tau roles—are coordinately partitioned by isoform expression, phosphorylation, and conformational state across tissues remains unresolved.
  • No unified model linking isoform/phospho-state to compartment-specific function
  • Causal contribution of BIN1 variants to Alzheimer's pathogenesis mechanism still debated across opposing neuronal/microglial datasets

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 4 GO:0005198 structural molecule activity 3 GO:0008092 cytoskeletal protein binding 3 GO:0008289 lipid binding 3 GO:0060090 molecular adaptor activity 3 GO:0140110 transcription regulator activity 2
Localization
GO:0005768 endosome 4 GO:0005886 plasma membrane 4 GO:0005634 nucleus 3 GO:0005856 cytoskeleton 3
Pathway
R-HSA-1852241 Organelle biogenesis and maintenance 4 R-HSA-397014 Muscle contraction 4 R-HSA-5653656 Vesicle-mediated transport 4 R-HSA-112316 Neuronal System 3 R-HSA-73894 DNA Repair 2
Partners
BACE1CAV1.2 (CACNA1C)CLIP-170DNM2E2F1MYCPARP1TAU (MAPT)
Complex memberships
BIN1-DNM2 membrane fission complexBIN1-EHD1/RME-1 recycling tubuleEHBP1L1-Rab8-BIN1-dynamin complexT-tubule/dyad scaffold (BIN1-Cav1.2-RyR2)

Evidence

Reading pass · 55 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1996 BIN1 physically interacts with the N-terminal Myc box regions of the MYC oncoprotein and inhibits malignant cell transformation by MYC. Genetic screen/protein interaction assay; ectopic expression growth suppression assay Nature genetics High 8782822
1997 BIN1 (Amphiphysin II) localizes to the cortical cytomatrix of axon initial segments and nodes of Ranvier in brain, and concentrates around T tubules in skeletal muscle, co-localizing with ankyrin3 splice variants. Immunofluorescence, subcellular fractionation, direct localization experiments in tissue sections The Journal of cell biology High 9182667
1997 BIN1 is a short-lived nuclear phosphoprotein (half-life ~2 h) with altered subcellular localization in tumor cells versus normal cells: predominantly nucleoplasmic in normal cells but shifted to a subnuclear compartment in tumor cells. Pulse-chase experiments, immunoprecipitation, immunofluorescence with monoclonal antibodies Cancer research Medium 9242458
1997 Alternative splicing of BIN1 controls its MYC-binding capacity; one alternatively spliced exon encodes part of the MYC-binding domain, and MyoD transactivates the BIN1 promoter accounting for high muscle expression. Gene cloning, exon mapping, promoter reporter assays, cell-type-specific RT-PCR The Journal of biological chemistry Medium 9395479
1998 BIN1 promotes skeletal muscle differentiation; overexpression accelerates differentiation while antisense BIN1 impairs differentiation of C2C12 myoblasts; during differentiation BIN1 protein relocates from nucleus to cytoplasm via isoform switching. Stable antisense/sense overexpression in C2C12 cells, morphological and marker analysis of differentiation, immunolocalization Molecular and cellular biology Medium 9418903
1999 BIN1 interacts with and inhibits c-Myc transactivation through its Myc-binding domain (MBD), and inhibits cell proliferation via both MYC-dependent and MYC-independent mechanisms involving the BAR, U1, and SH3 domains. Co-immunoprecipitation, transcriptional reporter assays, Ras cotransformation assay, growth inhibition assays Oncogene High 10380878
1999 Aberrant inclusion of brain-specific exon 12A in BIN1 abrogates its ability to inhibit malignant transformation by c-Myc or adenovirus E1A and eliminates its ability to induce programmed cell death in melanoma cells. RT-PCR isoform analysis, ectopic expression of exon 12A-containing vs. non-containing BIN1 in transformation and apoptosis assays Proceedings of the National Academy of Sciences of the United States of America Medium 10449755
2000 BIN1 engages a caspase-independent cell death program characterized by cell shrinkage, vacuolated cytoplasm, and DNA degradation; this is abrogated by BAR domain mutation or the melanoma-associated exon 12A missplicing event, and is not blocked by Bcl-2 or caspase inhibitor ZVAD.fmk. Ectopic expression, domain mutagenesis, pharmacological inhibitors, cell death assays Oncogene Medium 11032017
2001 BIN1 mediates c-Myc-induced apoptosis in transformed primary cells via protein-protein interaction; antisense or dominant-inhibitory BIN1 reduced susceptibility to c-Myc-induced apoptosis without affecting proliferation or transformation. Antisense/dominant-negative expression, apoptosis assays in chick and rat primary transformed cells Cancer research Medium 11306501
2003 Constitutive knockout of murine Bin1 causes perinatal lethality with severe ventricular cardiomyopathy and disorganized myofibrils, but does not impair endocytosis, phagocytosis, actin organization, proliferation, or apoptosis in fibroblasts/macrophages. Homologous recombination knockout mouse, histology, electron microscopy, endocytosis/phagocytosis assays Molecular and cellular biology High 12773571
2004 The Exon10 (polybasic) sequence of BIN1 binds PI(4,5)P2 and also intramolecularly binds the BIN1 SH3 domain, blocking SH3-mediated binding to PxxP ligands (including dynamin); this blockage is released by PI(4,5)P2, providing a phosphoinositide-regulated SH3 domain mechanism. In vitro binding assays, PI(4,5)P2 addition/depletion experiments, cell overexpression with PI4P5-kinase, T-tubule formation assay The EMBO journal High 15483625
2005 The BIN1 SH3 domain binds a class II SH3-binding motif in c-Myc; tumor-specific BIN1 isoforms are prevented from binding c-Myc via an intramolecular polyproline-SH3 interaction; phosphorylation of c-Myc at Ser62 inhibits BIN1 binding. NMR structure, surface plasmon resonance, biochemical binding assays, structure-based modeling Journal of molecular biology High 15992821
2006 Crystal structure of the BIN1 BAR domain at 2.0 Å resolution reveals a homodimeric crescent-shaped architecture with knobs-into-holes coiled-coil packing governing membrane-engaging concave face curvature, and identifies two potential protein-protein interaction sites on the convex face. X-ray crystallography Biochemistry High 17059209
2006 HCV NS5A protein interacts with BIN1 via NS5A's SH3-binding motif (PxxP) and BIN1's SH3 domain; this interaction inhibits BIN1-induced apoptosis and is required for productive HCV infection. Yeast two-hybrid, in vitro binding, co-immunoprecipitation, confocal microscopy, deletion/mutation analysis, HCV infectivity in chimpanzees Gastroenterology Medium 16530520
2008 BIN1's membrane-tubulating activity depends on CLIP-170; BIN1 interacts with CLIP-170 via its BAR domain and CLIP-170's coiled-coil region; depletion of CLIP-170 reduces BIN1-induced tubule formation, and BIN1 tubules align with microtubules. Yeast two-hybrid, GST pulldown, co-immunoprecipitation, RNAi knockdown, confocal microscopy, nocodazole depolymerization European journal of cell biology Medium 19004523
2009 BIN1 (AMPH-1 in C. elegans) colocalizes with RME-1/EHD1 on recycling endosomes; BIN1/AMPH-1 deletion impairs recycling endosome function; purified AMPH-1 and RME-1 together form coated membrane tubules distinct from those produced by either alone, and BIN1 is required for EHD1-regulated endocytic recycling in human cells. In vivo co-localization, deletion mutant analysis, in vitro reconstitution of membrane tubules, siRNA knockdown in human cells Nature cell biology High 19915558
2009 BIN1's SH3 domain acts as a scaffold for sarcomere assembly; it forms transient complexes with actin, myosin filaments, and the pro-myogenic kinase Cdk5, and associates with a Cdk5 phosphorylation domain of titin; expression of isolated SH3 domain causes myofiber disorganization. Dominant-negative expression in mouse, co-immunoprecipitation, pull-down assays The Journal of biological chemistry Medium 19633357
2009 BIN1 is a transcriptional target of E2F1 (via canonical E2F sites in the BIN1 promoter) and mediates E2F1-induced apoptosis in response to DNA damage; BIN1 suppression attenuates E2F1/etoposide-induced cell death independently of p53, p73, and caspases. Promoter reporter assay, ChIP, siRNA knockdown, antisense suppression, DNA damage apoptosis assays Cell death and differentiation Medium 19629135
2010 BIN1 localizes to cardiac T-tubules and clusters there with L-type calcium channel Cav1.2; dynamic microtubules tethered to BIN1-scaffolded membrane enable targeted delivery of Cav1.2; BIN1 knockdown reduces surface Cav1.2 and delays calcium transient development. Immunocytochemistry, electron microscopy with dual immunogold labeling, co-immunoprecipitation, surface biotinylation, live-cell confocal/TIRF microscopy, shRNA knockdown PLoS biology High 20169111
2011 BIN1 is required for skeletal muscle T-tubule biogenesis and excitation-contraction coupling; Bin1 knockdown in adult mouse skeletal muscle disrupts T-tubule structure, reduces DHPR-RyR1 coupling, and impairs SR Ca2+ release. In vivo electroporation shRNA delivery, confocal imaging of T-tubules, patch-clamp, Ca2+ spark and transient measurements PloS one Medium 21984944
2011 BIN1 is significantly reduced (~36% protein) in failing human cardiomyocytes, and this reduction impairs Cav1.2 trafficking to T-tubules, reduces calcium transients, and causes contractile dysfunction (75% reduction in calcium transients in BIN1-knockdown zebrafish hearts). Human failing heart tissue analysis, immunostaining, biochemical fractionation, patch-clamp in cell lines, mouse cardiomyocyte shRNA knockdown, zebrafish morpholino knockdown Heart rhythm High 22138472
2011 MBNL1 binds the BIN1 pre-mRNA and regulates its alternative splicing; in myotonic dystrophy, BIN1 missplicing produces an inactive BIN1 form lacking PI(5)P-binding and membrane-tubulating activities, causing T-tubule alterations; reproducing this splicing alteration in mice is sufficient to cause T-tubule defects and muscle weakness. RNA binding assay (MBNL1-BIN1 pre-mRNA), functional splicing assays, rescue experiments in patient muscle cells, transgenic mouse model Nature medicine High 21623381
2011 BIN1 binds the automodification domain of PARP1 and suppresses its catalytic activity; c-MYC represses BIN1 expression (by blocking MIZ1-mediated activation) thereby releasing PARP1 activity and increasing cisplatin resistance. Co-immunoprecipitation, PARP1 activity assay, siRNA knockdown, promoter analysis, cisplatin resistance assays Science signaling High 21447800
2012 BIN1 interacts with Tau and they co-localize in human neuroblastoma cells and mouse brain; decreased expression of the Drosophila BIN1 ortholog Amph suppresses Tau-mediated neurotoxicity in three assays. Co-immunoprecipitation, co-localization confocal microscopy, Drosophila genetic suppressor assay Molecular psychiatry Medium 23399914
2012 Transient structure in the intrinsically disordered c-Myc transactivation domain (residues 22-33 and MB1) mediates binding to BIN1 SH3 domain; Bin1 binds primarily to the Ser62 region of c-Myc in a dynamically disordered, multivalent complex; binding causes population shifts in Myc conformational dynamics. NMR chemical shift analysis, relaxation measurements, NOE analysis, surface plasmon resonance Nucleic acids research High 22457068
2013 BIN1 directly interacts with Tau through its SH3 domain binding Tau's proline-rich domain; BIN1-Tau complexes co-localize with the actin cytoskeleton in primary neurons; phosphorylation of Tau at Thr231 weakens the SH3-PRD interaction. GST pulldown, NMR, co-localization in primary neurons, phospho-specific analysis Acta neuropathologica communications High 26395440
2014 A cardiac-specific isoform of BIN1 (BIN1+13+17) promotes N-WASP-dependent actin polymerization to fold T-tubule inner membranes at Z-discs, creating a 'fuzzy space' that restricts local ion diffusion; cardiac Bin1 deletion decreases T-tubule folding, allowing free diffusion of Ca2+ and K+, prolonging action potential duration and increasing arrhythmia susceptibility. Cardiac-specific Bin1 knockout mouse, electrophysiology, superresolution microscopy, BIN1 isoform re-expression, N-WASP interaction assay Nature medicine High 24836577
2014 BIN1/M-Amphiphysin2 clusters PtdIns(4,5)P2 on membranes to recruit dynamin; the N-BAR domain controls kinetics and accumulation of dynamin while the SH3 domain controls its accumulation on membranes; CNM-associated BIN1 mutants show defects in this process. In vitro membrane binding assays, fluorescence microscopy, CNM mutant analysis, numerical simulations Nature communications High 25487648
2014 BIN1 knockdown by siRNA reduces t-tubule density, calcium transient amplitude, and synchrony of systolic calcium transient in rat cardiac cells; AmpII protein levels correlate with t-tubule density across cardiac chambers and heart failure models. siRNA knockdown, di-4-ANEPPS/FM4-64 t-tubule staining, calcium transient measurements, Western blot across species/chamber comparisons Circulation research Medium 25332206
2014 BIN1 membrane curvature sensing and generation is autoinhibited by intramolecular exon10-SH3 interaction on membranes lacking PI(4,5)P2; addition of PI(4,5)P2 or SH3 domain ligands (PRD peptides) relieves autoinhibition and activates BIN1 membrane deformation. In vitro membrane deformation assays, SH3-exon10 binding assays, PI(4,5)P2 titration, CNM mutant analysis Biochemistry High 25350771
2014 CNM-associated BIN1 mutants R154Q and D151N disrupt membrane tubulation through distinct mechanisms: R154Q reduces membrane-bound protein density while D151N impairs protein oligomerization upon membrane binding. In vitro liposome tubulation assays, protein density quantification, chemical crosslinking, live-cell depolymerization assays PloS one Medium 24755653
2015 Cryo-EM reveals that BIN1 N-BAR domains self-assemble cooperatively on membrane tubes; the N-terminal amphipathic helix H0 initiates tube assembly and organizes BAR-mediated polymerization by locking adjacent N-BAR domains; loss of H0 or BAR tip disrupts polymer organization. Cryo-EM, 3D reconstruction, biochemical tubulation assays with mutants Scientific reports High 26487375
2016 Isoproterenol (β-adrenergic activation) promotes BIN1 redistribution to T-tubules and recruits phosphorylated RyR2 (P-RyR) into BIN1+13+17-organized dyads via coimmunoprecipitation; in cardiac-specific Bin1 heterozygous mice, isoproterenol fails to concentrate BIN1 and recruit P-RyRs, resulting in uncoupled P-RyRs and spontaneous calcium release. Co-immunoprecipitation, superresolution fluorescent imaging, cardiac-specific Bin1 heterozygous mice, calcium imaging Circulation Medium 26733606
2016 EHBP1L1 directly binds GTP-loaded Rab8 and BIN1, forming a complex with dynamin at the endocytic recycling compartment; this complex is required for apical-directed vesicular transport in polarized epithelial cells. Co-immunoprecipitation, biochemical binding assays, overexpression/knockdown in organoids and cells, EHBP1L1-KO mouse The Journal of cell biology Medium 26833786
2017 BIN1 acts as a negative regulator of Dynamin 2 (DNM2) during muscle maturation; Bin1-/- Dnm2+/- double-null mice survive (unlike Bin1-/- alone) with normal muscle; BIN1 colocalized with and partially inhibited DNM2 activity in vitro during muscle maturation but not for the adult muscle DNM2 isoform. Genetic epistasis (Bin1-/- Dnm2+/- mice), in vitro DNM2 GTPase activity assay, co-localization The Journal of clinical investigation High 29130937
2017 Bin1 BAR domain directly binds actin filaments, has moderate actin bundling activity, stabilizes actin filaments against depolymerization, and stabilizes tau-induced actin bundles; knockdown of Bin1 in a Drosophila tauopathy model reduces tau-induced actin inclusions. In vitro actin binding/bundling assays, F-actin co-sedimentation, Drosophila genetic KD with actin inclusion quantification EMBO reports Medium 28893863
2018 NMR structural model of BIN1 SH3 domain binding to Tau peptide (213-229) shows P216 and P219 contact BIN1 SH3 aromatic residues F588 and W562, while R221 and K224 of Tau form electrostatic contacts with E556/E557 of BIN1; phosphorylation of Tau at T212, T217, T231, and S235 reduces interaction with BIN1 SH3 five-fold (Kd 44 to 256 μM) and prevents Tau from competing with BIN1's intramolecular SH3-CLAP interaction. NMR spectroscopy, SPR, competition binding assays Frontiers in molecular neuroscience High 30487734
2018 BIN1 expression in hESC-derived cardiomyocytes induces T-tubule development, promotes Cav1.2 clustering along T-tubules, increases coupled Cav1.2 gating probability, anchors sarcoplasmic reticulum, and increases Cav1.2-RyR junctions. BIN1 transfection in hESC-CMs, electrophysiology, superresolution microscopy, Ca2+ imaging Stem cells Medium 30353632
2019 BIN1 interacts with Tau via SH3-PRD interaction; calcineurin dephosphorylates BIN1 at Thr348 (a CDK phosphorylation site), promoting open BIN1 conformation and increasing availability of SH3 domain for Tau binding as demonstrated by NMR; phospho-BIN1(T348):BIN1 ratio is increased in AD brains. NMR spectroscopy, high-content screening (1126 compounds), calcineurin inhibitor pharmacology, primary neuron experiments, AD brain biochemistry Acta neuropathologica High 31065832
2019 BIN1 loss in microglia reduces Tau secretion via extracellular vesicles in vitro and decreases Tau spreading in vivo in male PS19 mice; microglial Bin1 deletion reduces heat-shock protein expression previously linked to Tau proteostasis. Cre-lox microglia-specific Bin1 conditional knockout, extracellular vesicle Tau quantification, in vivo tau spreading assay in PS19 mice Scientific reports Medium 31263146
2019 BIN1 interacts with L-type voltage-gated calcium channels (LVGCCs) in rat hippocampal neurons and mouse brain; BIN1-LVGCC interactions are modulated by Tau; increasing neuronal BIN1 expression induces network hyperexcitability and increased calcium transients; Tau reduction prevents BIN1-induced hyperexcitability. Co-immunoprecipitation, multielectrode array recordings, calcium imaging, Tau reduction genetic experiments in hippocampal neurons eLife Medium 32657270
2019 Neuronal BIN1 loss-of-function impairs spatial learning and memory, reduces presynaptic release probability, alters synaptic vesicle dynamics (increases docked and reserve vesicle pools), reduces synapse density, and alters presynaptic active zone protein clustering; BIN1 localizes to presynaptic sites by superresolution and immunoelectron microscopy. Conditional neuronal Bin1 KO, fear conditioning/Morris water maze, electrophysiology, superresolution microscopy, immunoelectron microscopy, 3D-EM reconstruction Cell reports High 32160554
2019 BIN1 loss-of-function in neurons does not regulate Aβ generation in vivo: 50% global BIN1 reduction or conditional neuronal BIN1 KO does not alter BACE1 levels, localization, APP processing, or Aβ deposition in the 5XFAD mouse model. Bin1 heterozygous KO mice, conditional neuronal KO, 5XFAD amyloidosis model, biochemistry, immunohistochemistry The Journal of biological chemistry Medium 30692199
2019 BIN1 loss enables ATM activation via E2F1: BIN1 inactivates ATM kinase particularly when bound to E2F1; BIN1 prevents E2F1 from transcriptionally activating the ATM promoter; BIN1 loss increases MRE11A/RAD50/NBS1 complex formation and promotes ATM autophosphorylation and γH2AX, driving cisplatin resistance. Co-immunoprecipitation, promoter reporter assays, siRNA knockdown, ATM kinase assays, cisplatin sensitivity assays The Journal of biological chemistry Medium 30733337
2020 BIN1 localizes to postsynaptic compartments (dendritic spines); participates in protein complexes with Arf6 and GluA1; manipulations of BIN1 alter AMPA receptor surface expression, trafficking, spine morphology, and AMPA receptor-mediated synaptic transmission. Superresolution SIM microscopy, co-immunoprecipitation, AMPA receptor surface biotinylation, electrophysiology, BIN1 knockdown/overexpression Molecular psychiatry Medium 30967682
2020 Neuronal Bin1 conditional KO reduces neuronal excitability in vitro and alters microglial transcriptome in vivo; in PS19 Tau transgenic background, neuronal Bin1 loss increases mortality without worsening neuropathology. Conditional KO (Thy1-Cre x Bin1flox/flox x PS19), electrophysiology in primary neurons, c-fos immunostaining, microglial transcriptomics PloS one Medium 31408457
2020 Dynamin 2 GTPase activity is suppressed through interaction with BIN1; CNM disease-associated mutant dynamin 2 retains active GTPase due to lack of BIN1-mediated regulation, causing aberrant membrane fission and remodeling in T-tubule equivalent structures. In cellulo reconstitution assay, GTPase activity measurements, BIN1-DNM2 interaction assays, CNM mutant analysis The Journal of biological chemistry High 33187981
2021 Muscle-specific Bin1 knockout (Bin1mck-/-) mice recapitulate centronuclear myopathy with T-tubule and mitochondria network defects and impaired calcium homeostasis; DNM2 antisense oligonucleotides rescue force and histology, confirming BIN1-DNM2 functional pathway in vivo. Muscle-specific conditional KO, force measurements, histology, electron microscopy, Ca2+ homeostasis, ASO treatment Molecular therapy High 34371181
2021 Cavin4 directly interacts with Bin1 (Cavin4b in zebrafish) and localizes to T-tubules; loss of Cavin4 causes aberrant T-tubule maturation and accumulation of interconnected caveolae within T-tubules, impairing Ca2+ response; Cavin4 is proposed to remodel T-tubule membrane by recycling caveolar components. Direct interaction assay (Cavin4b-Bin1), Cavin4 KO mouse and zebrafish, confocal microscopy, Ca2+ imaging The Journal of cell biology Medium 34633413
2021 LOAD-associated BIN1 coding variants (rs754834233, rs138047593) reduce BIN1 interaction with BACE1 and fail to rescue BACE1 recycling impaired by Bin1 knockdown; BIN1 normally promotes BACE1 recycling from early endosomes, and its loss increases intracellular Aβ42 by enabling BACE1 cleavage of APP in enlarged early endosomes. Co-immunoprecipitation of BIN1-BACE1, early endosome morphology assay, Aβ ELISA, BACE1 recycling assay, BIN1 variant overexpression/knockdown rescue The Journal of biological chemistry Medium 34375641
2022 BIN1 isoform 1 (neuronal) overexpression causes early endosome accumulation and neurodegeneration in Drosophila and human induced neurons; BIN1 KO narrows early endosomes; isoform 1 rescues BIN1-KO early endosome phenotype but isoform 9 does not, identifying isoform-specific control of early endosome size. Drosophila overexpression, hiN and cerebral organoid BIN1 KO, endosome morphometry, isoform rescue experiments Acta neuropathologica communications Medium 34998435
2022 Neuronal BIN1 isoform 1 (BIN1V1) but not isoform 9 (BIN1V9) downregulates BACE1-mediated APP processing and Aβ generation in a RIN3-dependent manner; BIN1V1 delays APP (but not BACE1) endocytosis into early endosomes, spatially separating APP from BACE1; RIN3 sequesters BIN1V1 via CLAP domain into RAB5+ endosomes. Confocal microscopy, Western blot, Aβ ELISA, surface biotinylation APP internalization assay, FACS-enriched cell populations, isoform transfection Scientific reports Medium 35241726
2022 Microglial BIN1 regulates proinflammatory and disease-associated microglial activation; BIN1 loss impairs type 1 interferon responses in microglia, particularly upregulation of Ifitm3; Bin1 regulates transcription factors PU.1 and IRF1, and loss of microglial Bin1 in vivo alters disease-associated gene expression and CX3CR1 signaling. siRNA Bin1 knockdown in primary microglia, Cre-lox microglia-specific conditional KO, NanoString transcriptomics, flow cytometry, cytokine measurement Molecular neurodegeneration Medium 35526014
2023 BIN1, MTM1, and DNM2 have balanced roles in T-tubule growth in cardiomyocytes: all four cardiac BIN1 isoforms induce tubulation but with different geometries; high MTM1 (phosphoinositide phosphatase) levels are necessary for BIN1-induced tubulation (without direct binding to cardiac BIN1 isoforms lacking exon 11); high DNM2 levels are inhibitory for T-tubule formation despite DNM2 binding all four BIN1 isoforms and co-localizing at Z-lines. Developing mouse cardiomyocytes, gene-modified HL-1 and hiPSC-derived cardiomyocytes, confocal/Airyscan microscopy, RT-qPCR, Western blot, Ca2+ recording, co-IP Circulation research Medium 37139790
2005 Bin1 loss elevates STAT1- and NF-κB-dependent expression of IDO in tumor cells, promoting immune escape from T cell-dependent antitumor immunity; Bin1 knockout studies establish that Bin1 loss upregulates IDO expression. Mouse knockout studies, STAT1/NF-κB pathway analysis, IDO expression measurement, immune escape assays in MMTV-Neu mice Nature medicine Medium 15711557

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Inhibition of indoleamine 2,3-dioxygenase, an immunoregulatory target of the cancer suppression gene Bin1, potentiates cancer chemotherapy. Nature medicine 891 15711557
2014 Alzheimer's disease: early alterations in brain DNA methylation at ANK1, BIN1, RHBDF2 and other loci. Nature neuroscience 743 25129075
1996 BIN1 is a novel MYC-interacting protein with features of a tumour suppressor. Nature genetics 323 8782822
2013 Increased expression of BIN1 mediates Alzheimer genetic risk by modulating tau pathology. Molecular psychiatry 312 23399914
2011 Misregulated alternative splicing of BIN1 is associated with T tubule alterations and muscle weakness in myotonic dystrophy. Nature medicine 268 21623381
2015 Association of Brain DNA methylation in SORL1, ABCA7, HLA-DRB5, SLC24A4, and BIN1 with pathological diagnosis of Alzheimer disease. JAMA neurology 238 25365775
1997 Amphiphysin II (SH3P9; BIN1), a member of the amphiphysin/Rvs family, is concentrated in the cortical cytomatrix of axon initial segments and nodes of ranvier in brain and around T tubules in skeletal muscle. The Journal of cell biology 226 9182667
2014 Cardiac BIN1 folds T-tubule membrane, controlling ion flux and limiting arrhythmia. Nature medicine 202 24836577
2010 BIN1 localizes the L-type calcium channel to cardiac T-tubules. PLoS biology 186 20169111
2009 AMPH-1/Amphiphysin/Bin1 functions with RME-1/Ehd1 in endocytic recycling. Nature cell biology 168 19915558
1999 Mechanism for elimination of a tumor suppressor: aberrant splicing of a brain-specific exon causes loss of function of Bin1 in melanoma. Proceedings of the National Academy of Sciences of the United States of America 162 10449755
2013 Bridging integrator 1 (BIN1): form, function, and Alzheimer's disease. Trends in molecular medicine 151 23871436
2022 Hypoxia-induced exosomal circPDK1 promotes pancreatic cancer glycolysis via c-myc activation by modulating miR-628-3p/BPTF axis and degrading BIN1. Journal of hematology & oncology 143 36068586
2019 BIN1 favors the spreading of Tau via extracellular vesicles. Scientific reports 137 31263146
2011 BIN1 is reduced and Cav1.2 trafficking is impaired in human failing cardiomyocytes. Heart rhythm 136 22138472
1997 Structural analysis of the human BIN1 gene. Evidence for tissue-specific transcriptional regulation and alternate RNA splicing. The Journal of biological chemistry 130 9395479
2014 Amphiphysin 2 (BIN1) in physiology and diseases. Journal of molecular medicine (Berlin, Germany) 128 24590001
2016 Predominant expression of Alzheimer's disease-associated BIN1 in mature oligodendrocytes and localization to white matter tracts. Molecular neurodegeneration 108 27488240
2014 Dependence of cardiac transverse tubules on the BAR domain protein amphiphysin II (BIN-1). Circulation research 108 25332206
2003 Targeted disruption of the murine Bin1/Amphiphysin II gene does not disable endocytosis but results in embryonic cardiomyopathy with aberrant myofibril formation. Molecular and cellular biology 106 12773571
1999 Bin1 functionally interacts with Myc and inhibits cell proliferation via multiple mechanisms. Oncogene 104 10380878
2000 The c-Myc-interacting adaptor protein Bin1 activates a caspase-independent cell death program. Oncogene 98 11032017
1998 A role for the putative tumor suppressor Bin1 in muscle cell differentiation. Molecular and cellular biology 97 9418903
2012 Mechanisms of Cisplatin-Induced Apoptosis and of Cisplatin Sensitivity: Potential of BIN1 to Act as a Potent Predictor of Cisplatin Sensitivity in Gastric Cancer Treatment. International journal of surgical oncology 93 22778941
2014 BIN1/M-Amphiphysin2 induces clustering of phosphoinositides to recruit its downstream partner dynamin. Nature communications 90 25487648
2012 Transient structure and dynamics in the disordered c-Myc transactivation domain affect Bin1 binding. Nucleic acids research 90 22457068
2005 A structure-based model of the c-Myc/Bin1 protein interaction shows alternative splicing of Bin1 and c-Myc phosphorylation are key binding determinants. Journal of molecular biology 90 15992821
2022 BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia. Molecular neurodegeneration 87 35526014
2011 c-MYC suppresses BIN1 to release poly(ADP-ribose) polymerase 1: a mechanism by which cancer cells acquire cisplatin resistance. Science signaling 85 21447800
2020 Neuronal BIN1 Regulates Presynaptic Neurotransmitter Release and Memory Consolidation. Cell reports 84 32160554
2017 BIN1 reverses PD-L1-mediated immune escape by inactivating the c-MYC and EGFR/MAPK signaling pathways in non-small cell lung cancer. Oncogene 84 28714960
2019 The BIN1 rs744373 SNP is associated with increased tau-PET levels and impaired memory. Nature communications 79 30992433
2014 Adult-onset autosomal dominant centronuclear myopathy due to BIN1 mutations. Brain : a journal of neurology 78 25260562
2000 Losses of the tumor suppressor BIN1 in breast carcinoma are frequent and reflect deficits in programmed cell death capacity. International journal of cancer 78 10652430
2000 Loss of heterozygosity and tumor suppressor activity of Bin1 in prostate carcinoma. International journal of cancer 78 10738240
2017 Amphiphysin (BIN1) negatively regulates dynamin 2 for normal muscle maturation. The Journal of clinical investigation 72 29130937
2016 Isoproterenol Promotes Rapid Ryanodine Receptor Movement to Bridging Integrator 1 (BIN1)-Organized Dyads. Circulation 72 26733606
2015 Tau phosphorylation regulates the interaction between BIN1's SH3 domain and Tau's proline-rich domain. Acta neuropathologica communications 72 26395440
2001 Bin1 mediates apoptosis by c-Myc in transformed primary cells. Cancer research 68 11306501
2006 The crystal structure of the BAR domain from human Bin1/amphiphysin II and its implications for molecular recognition. Biochemistry 66 17059209
2004 Regulation of Bin1 SH3 domain binding by phosphoinositides. The EMBO journal 66 15483625
2007 Bin1 ablation increases susceptibility to cancer during aging, particularly lung cancer. Cancer research 62 17699764
2013 BIN1 is decreased in sporadic but not familial Alzheimer's disease or in aging. PloS one 60 24205320
2020 Alzheimer's disease risk gene BIN1 induces Tau-dependent network hyperexcitability. eLife 57 32657270
2013 Altered splicing of the BIN1 muscle-specific exon in humans and dogs with highly progressive centronuclear myopathy. PLoS genetics 57 23754947
2006 The SH3 binding motif of HCV [corrected] NS5A protein interacts with Bin1 and is important for apoptosis and infectivity. Gastroenterology 55 16530520
2003 Expression of a MYCN-interacting isoform of the tumor suppressor BIN1 is reduced in neuroblastomas with unfavorable biological features. Clinical cancer research : an official journal of the American Association for Cancer Research 55 12960121
2016 EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells. The Journal of cell biology 53 26833786
2012 Plasma BIN1 correlates with heart failure and predicts arrhythmia in patients with arrhythmogenic right ventricular cardiomyopathy. Heart rhythm 51 22300662
2019 BIN1 recovers tauopathy-induced long-term memory deficits in mice and interacts with Tau through Thr348 phosphorylation. Acta neuropathologica 50 31065832
2022 The Alzheimer susceptibility gene BIN1 induces isoform-dependent neurotoxicity through early endosome defects. Acta neuropathologica communications 49 34998435
2019 A novel role for the late-onset Alzheimer's disease (LOAD)-associated protein Bin1 in regulating postsynaptic trafficking and glutamatergic signaling. Molecular psychiatry 49 30967682
2011 Disrupted membrane structure and intracellular Ca²⁺ signaling in adult skeletal muscle with acute knockdown of Bin1. PloS one 49 21984944
2015 Bridging Integrator 1 (BIN1) Genotype Effects on Working Memory, Hippocampal Volume, and Functional Connectivity in Young Healthy Individuals. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 47 25630570
1999 Induction of apoptosis and differentiation in neuroblastoma and astrocytoma cells by the overexpression of Bin1, a novel Myc interacting protein. Journal of cellular biochemistry 47 10412034
2020 BIN1 protein isoforms are differentially expressed in astrocytes, neurons, and microglia: neuronal and astrocyte BIN1 are implicated in tau pathology. Molecular neurodegeneration 45 32727516
2018 BIN1 Induces the Formation of T-Tubules and Adult-Like Ca2+ Release Units in Developing Cardiomyocytes. Stem cells (Dayton, Ohio) 45 30353632
2015 Structural insights into the cooperative remodeling of membranes by amphiphysin/BIN1. Scientific reports 45 26487375
1997 The putative tumor suppressor BIN1 is a short-lived nuclear phosphoprotein, the localization of which is altered in malignant cells. Cancer research 44 9242458
2017 Bin1 directly remodels actin dynamics through its BAR domain. EMBO reports 43 28893863
2010 Case report of intrafamilial variability in autosomal recessive centronuclear myopathy associated to a novel BIN1 stop mutation. Orphanet journal of rare diseases 43 21129173
2014 Mutations in BIN1 associated with centronuclear myopathy disrupt membrane remodeling by affecting protein density and oligomerization. PloS one 41 24755653
2008 The membrane-tubulating potential of amphiphysin 2/BIN1 is dependent on the microtubule-binding cytoplasmic linker protein 170 (CLIP-170). European journal of cell biology 41 19004523
2014 Genetic variation in BIN1 gene and Alzheimer's disease risk in Han Chinese individuals. Neurobiology of aging 40 24582639
2014 The benefits of staying active in old age: physical activity counteracts the negative influence of PICALM, BIN1, and CLU risk alleles on episodic memory functioning. Psychology and aging 40 24660791
2018 Structural Basis of Tau Interaction With BIN1 and Regulation by Tau Phosphorylation. Frontiers in molecular neuroscience 39 30487734
2015 BIN1 regulates dynamic t-tubule membrane. Biochimica et biophysica acta 38 26578114
2013 BIN1 gene rs744373 polymorphism contributes to Alzheimer's disease in East Asian population. Neuroscience letters 38 23570733
2003 Immunohistochemical analysis of Bin1/Amphiphysin II in human tissues: diverse sites of nuclear expression and losses in prostate cancer. Journal of cellular biochemistry 38 12532338
2007 Bin1 ablation in mammary gland delays tissue remodeling and drives cancer progression. Cancer research 37 17210688
2021 Mice with muscle-specific deletion of Bin1 recapitulate centronuclear myopathy and acute downregulation of dynamin 2 improves their phenotypes. Molecular therapy : the journal of the American Society of Gene Therapy 35 34371181
2017 Regulation of the interaction between the neuronal BIN1 isoform 1 and Tau proteins - role of the SH3 domain. The FEBS journal 35 28755476
2009 The c-MYC-interacting proapoptotic tumor suppressor BIN1 is a transcriptional target for E2F1 in response to DNA damage. Cell death and differentiation 34 19629135
2023 Loss of forebrain BIN1 attenuates hippocampal pathology and neuroinflammation in a tauopathy model. Brain : a journal of neurology 33 36059072
2019 Reduction of the expression of the late-onset Alzheimer's disease (AD) risk-factor BIN1 does not affect amyloid pathology in an AD mouse model. The Journal of biological chemistry 33 30692199
2017 Post-Myocardial Infarction T-tubules Form Enlarged Branched Structures With Dysregulation of Junctophilin-2 and Bridging Integrator 1 (BIN-1). Journal of the American Heart Association 31 28473402
2009 Identification of tyrosine kinase, HCK, and tumor suppressor, BIN1, as potential mediators of AHI-1 oncogene in primary and transformed CTCL cells. Blood 31 19211505
2015 Cardiac-specific disruption of Bin1 in mice enables a model of stress- and age-associated dilated cardiomyopathy. Journal of cellular biochemistry 30 25939245
2014 BIN1 membrane curvature sensing and generation show autoinhibition regulated by downstream ligands and PI(4,5)P2. Biochemistry 30 25350771
1999 The murine Bin1 gene functions early in myogenesis and defines a new region of synteny between mouse chromosome 18 and human chromosome 2. Genomics 29 10036185
2021 Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle. The Journal of cell biology 28 34633413
2016 Structural insights into the centronuclear myopathy-associated functions of BIN1 and dynamin 2. Journal of structural biology 28 27343996
2009 Bin1 SRC homology 3 domain acts as a scaffold for myofiber sarcomere assembly. The Journal of biological chemistry 28 19633357
2022 Long non-coding RNA SNHG10 upregulates BIN1 to suppress the tumorigenesis and epithelial-mesenchymal transition of epithelial ovarian cancer via sponging miR-200a-3p. Cell death discovery 27 35149697
2021 Alzheimer's disease BIN1 coding variants increase intracellular Aβ levels by interfering with BACE1 recycling. The Journal of biological chemistry 27 34375641
2020 Differential physiological roles for BIN1 isoforms in skeletal muscle development, function and regeneration. Disease models & mechanisms 27 32994313
2020 Mutant BIN1-Dynamin 2 complexes dysregulate membrane remodeling in the pathogenesis of centronuclear myopathy. The Journal of biological chemistry 27 33187981
2019 SRSF1-dependent alternative splicing attenuates BIN1 expression in non-small cell lung cancer. Journal of cellular biochemistry 27 31478261
2018 Aberrant accrual of BIN1 near Alzheimer's disease amyloid deposits in transgenic models. Brain pathology (Zurich, Switzerland) 27 30506549
2015 BIN1 tumor suppressor regulates Fas/Fas ligand-mediated apoptosis through c-FLIP in cutaneous T-cell lymphoma. Leukemia 27 25578476
2022 The neuronal-specific isoform of BIN1 regulates β-secretase cleavage of APP and Aβ generation in a RIN3-dependent manner. Scientific reports 25 35241726
2003 hob1+, the fission yeast homolog of Bin1, is dispensable for endocytosis or actin organization, but required for the response to starvation or genotoxic stress. Oncogene 25 12569356
2023 BIN1, Myotubularin, and Dynamin-2 Coordinate T-Tubule Growth in Cardiomyocytes. Circulation research 24 37139790
2019 Loss of the tumor suppressor BIN1 enables ATM Ser/Thr kinase activation by the nuclear protein E2F1 and renders cancer cells resistant to cisplatin. The Journal of biological chemistry 24 30733337
2017 Methylation decreases the Bin1 tumor suppressor in ESCC and restoration by decitabine inhibits the epithelial mesenchymal transition. Oncotarget 24 28152502
2013 Exploring the value of plasma BIN1 as a potential biomarker for alzheimer's disease. Journal of Alzheimer's disease : JAD 24 23803295
2014 Genetic evidence for the involvement of variants at APOE, BIN1, CR1, and PICALM loci in risk of late-onset Alzheimer's disease and evaluation for interactions with APOE genotypes. Journal of molecular neuroscience : MN 23 25022885
2007 Bin1 attenuation in breast cancer is correlated to nodal metastasis and reduced survival. Cancer biology & therapy 23 17218774
2000 BIN1 inhibits colony formation and induces apoptosis in neuroblastoma cell lines with MYCN amplification. Medical and pediatric oncology 23 11107117
2019 Cell-autonomous and non-cell autonomous effects of neuronal BIN1 loss in vivo. PloS one 21 31408457

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