Affinage

ABI1

Abl interactor 1 · UniProt Q8IZP0

Length
508 aa
Mass
55.1 kDa
Annotated
2026-06-09
98 papers in source corpus 50 papers cited in narrative 51 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/9 claims corpus-supported (89%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ABI1 (Abelson interactor 1/E3B1) is a multivalent cytoskeletal scaffold that couples Rho-family GTPase and tyrosine-kinase signaling to Arp2/3-dependent actin polymerization at protrusive structures (PMID:10499589, PMID:15048123). Its central function is to nucleate and stabilize the WAVE regulatory complex: ABI1 binds the WHD domain of WAVE2 and an N-terminal WAVE-binding domain of WAVE1 to stimulate WAVE-mediated actin polymerization and assemble the WAVE2-NAP1-PIR121 complex, and Abi1 loss in mice destabilizes WAVE1/WAVE2/NAP1/Sra-1, abolishes lamellipodial protrusion, and causes embryonic lethality with heart and brain malformations (PMID:15048123, PMID:15143189, PMID:21482783). In parallel, ABI1 acts as the scaffold of an EPS8-ABI1-SOS1 tri-complex (SH3 domain binding SOS1, proline-rich/PxxDY region binding EPS8) that, upon recruitment of the PI3K p85 subunit and PIP3, exhibits Rac-specific GEF activity downstream of Ras to drive actin remodeling (PMID:10499589, PMID:12515821, PMID:31488087). ABI1 also directly activates N-WASP cooperatively with Cdc42 to govern actin-based vesicular transport, EGFR endocytosis, and macropinocytosis, functions distinct from its WAVE-dependent membrane protrusion role (PMID:16155590). ABI1 allosterically regulates c-Abl: phospho-Y213 binds the Abl SH2 domain in trans while a proximal PXXP motif engages the Abl SH3 domain, and ABI1 reciprocally bridges c-Abl to WAVE2 phosphorylation (PMID:18328268, PMID:26428302). Its activity is gated by post-translational control including CDK1/cyclin B phosphorylation at S216 at mitosis, CaMKIIα phosphorylation at S88, and PTEN-mediated Y213/S216 dephosphorylation that triggers calpain-dependent degradation and reorganizes the actin cytoskeleton during epithelial polarization (PMID:21900237, PMID:22993434, PMID:32673396). Alternative splicing of exon 10 produces isoforms with opposite Rac1-binding and macropinocytosis behavior (PMID:20479892). Beyond actin, synaptically stimulated ABI1 translocates to the nucleus in an Abl-dependent manner to co-activate Myc/Max E-box transcription and shapes dendritic spine and synapse maturation (PMID:17304222). Loss of ABI1 in hematopoietic cells causes a myeloproliferative/myelofibrosis-like disease through derepressed SFK/STAT3/NF-κB signaling, and ABI1 loss promotes EMT, while ABI1 is a fusion partner of MLL in acute myeloid leukemia (PMID:9694699, PMID:30213875, PMID:31530281).

Mechanistic history

Synthesis pass · year-by-year structured walk · 28 steps
  1. 1997 Medium

    Established ABI1 as a phosphoprotein adaptor by showing it binds the SH3 domains of EPS8 and ABL and is hyperphosphorylated on serine after EGF stimulation, framing it as a receptor-coupled scaffold.

    Evidence SH3-domain library screen, in vitro binding and reciprocal Co-IP, phosphoamino acid analysis

    PMID:9010225

    Open questions at the time
    • Functional consequence of EPS8/ABL binding not yet defined
    • Kinase driving serine hyperphosphorylation unidentified
  2. 1998 Medium

    Identified ABI1 as an MLL fusion partner in t(10;11) AML, linking the scaffold to leukemogenesis at the structural level.

    Evidence RT-PCR cloning of chimeric MLL-ABI1 transcripts and chromosomal mapping in patient cases

    PMID:9694699

    Open questions at the time
    • Oncogenic mechanism of the fusion protein not tested
    • Contribution of ABI1 moiety vs MLL moiety unresolved
  3. 1999 High

    Showed ABI1 scaffolds an EPS8-SOS1 tri-complex with Rac-specific GEF activity, placing ABI1 mechanistically in the Ras→Rac signaling axis.

    Evidence In vivo Co-IP, in vitro Rac-GEF activity assay, epistatic signaling analysis

    PMID:10499589

    Open questions at the time
    • How GEF activity is switched on in cells not yet defined
    • Structural basis of complex assembly unknown
  4. 2003 High

    Defined the activation input for the tri-complex by showing PI3K p85 recruitment and PIP3 unmask its Rac-GEF activity, connecting lipid signaling to actin remodeling.

    Evidence Co-IP, in vitro Rac-GEF assay, dominant-negative and KO cell experiments

    PMID:12515821

    Open questions at the time
    • Quantitative stoichiometry of activation not measured
    • Spatial control of p85 recruitment unclear
  5. 2004 High

    Established ABI1 as a core WAVE-complex subunit by mapping direct WHD/WAB-domain binding to WAVE2/WAVE1, demonstrating it stimulates actin polymerization and is needed for lamellipodia.

    Evidence Mass spectrometry, in vitro actin polymerization assay, RNAi knockdown with live imaging, domain-mapping rescue in MEFs

    PMID:15048123 PMID:15143189

    Open questions at the time
    • In vivo organismal requirement not yet tested at this stage
    • SNARE-domain/Syntaxin-1 functional role undefined
  6. 2004 Low

    Identified calpain-dependent degradation of ABI1 controlled by Rac/PAK as a turnover mechanism, introducing proteolytic regulation of the scaffold.

    Evidence Calpain inhibitor treatment and constitutively active/dominant-negative PAK1/Rac1 constructs with Western blotting

    PMID:15178460

    Open questions at the time
    • Pharmacological/overexpression-only without reconstitution
    • Direct calpain cleavage site not mapped
  7. 2005 High

    Distinguished ABI1's two actin-nucleator arms by showing it directly activates N-WASP with Cdc42 for vesicular transport/EGFR endocytosis, separate from WAVE-driven membrane protrusion.

    Evidence In vitro actin polymerization with affinity measurement, RNAi with macropinocytosis and EGFR endocytosis readouts

    PMID:16155590

    Open questions at the time
    • How ABI1 partitions between WAVE and N-WASP pools unknown
    • Regulation of nucleator choice undefined
  8. 2005 Medium

    Extended ABI1's actin role to physiological migration by showing CSF-1-induced WAVE2-ABI1 co-recruitment drives macrophage protrusion and motility.

    Evidence Co-IP, RNAi knockdown, dominant-negative constructs, fluorescence microscopy

    PMID:16280551

    Open questions at the time
    • Upstream receptor-to-complex coupling not resolved
  9. 2007 Medium

    Revealed a nuclear/transcriptional function: synaptic NMDA stimulation drives Abl-dependent ABI1 translocation to nuclei where it co-activates Myc/Max, while it shapes dendritic spine maturation.

    Evidence Co-IP with Shank3/Eps8/SOS1, siRNA knockdown, nuclear translocation and E-box reporter assays in neurons

    PMID:17304222

    Open questions at the time
    • Direct DNA-bound complex composition not defined
    • Target genes activated in vivo unknown
  10. 2007 Medium

    Tied ABI1 to receptor downregulation by showing an EGF-inducible Abi1-Cbl complex required for EGFR internalization, disrupted by oncogenic Abl.

    Evidence Co-IP, SH3-deletion dominant-negative, EGFR internalization assay

    PMID:17395426

    Open questions at the time
    • Whether Cbl ubiquitinates ABI1 here not tested
    • Single-lab Co-IP
  11. 2008 High

    Provided the molecular mechanism by which ABI1 allosterically inhibits c-Abl, with pY213 engaging the Abl SH2 domain and a PXXP motif the SH3 domain in trans.

    Evidence In vitro kinase assays with phosphopeptides, Y213F mutagenesis, cellular co-expression

    PMID:18328268

    Open questions at the time
    • Cellular kinase generating pY213 not identified in this study
    • Reciprocal regulation context-dependence unclear
  12. 2010 Medium

    Connected ABI1 tyrosine phosphorylation to lipid signaling, showing pY213 binds the p85 C-terminal SH2 domain and is required for Abl-driven macropinocytosis.

    Evidence SH2 domain library screen, Y213 mutant, macropinocytosis assay

    PMID:20598684

    Open questions at the time
    • Direct biochemical affinity not quantified
  13. 2010 Medium

    Showed exon-10 alternative splicing functionally diversifies ABI1, with isoform 3 constitutively activating Rac1/macropinocytosis and isoform 2 binding activated Rac1 via the exon-10 site to suppress it.

    Evidence Macropinocytosis flow cytometry, Rac1 activation/binding assays, exon-10 domain mapping, fractionation

    PMID:20479892

    Open questions at the time
    • Tissue-specific splicing control not defined
    • Structural basis of differential Rac1 binding unknown
  14. 2010 Medium

    Demonstrated the tri-complex is required for cancer cell invasion in vivo, with ABI1 as the obligate scaffold holding SOS1 and EPS8 for LPA-induced Rac activation and ovarian metastasis.

    Evidence Co-IP, knockdown/re-expression rescue, in vivo peritoneal metastasis assay

    PMID:21118970

    Open questions at the time
    • Direct GEF activity in this metastatic setting not measured
  15. 2011 High

    Established ABI1 as essential for organismal development and WAVE-complex stability, with KO causing embryonic lethality and loss of WAVE/NAP1/Sra-1 protein.

    Evidence Conditional KO mouse, Western blotting, migration and ruffling assays

    PMID:21482783

    Open questions at the time
    • Tissue-autonomous requirements not dissected
    • Mechanism stabilizing partner proteins not fully resolved
  16. 2011 High

    Introduced cell-cycle control of ABI1, showing CDK1/cyclin B phosphorylates S216 at mitosis to attenuate Bcr-Abl-driven WAVE phosphorylation and F-actin assembly.

    Evidence In vitro kinase assay, S216D phosphomimetic, cell-cycle and F-actin assays

    PMID:21900237

    Open questions at the time
    • Phosphatase reversing S216 not identified here
  17. 2011 Medium

    Linked ABI1 to Bcr-Abl leukemogenesis, showing its knockdown reduces abnormal actin remodeling, adhesion/migration, Lyn activation, and leukemia in vivo.

    Evidence shRNA knockdown, actin/adhesion/migration assays, NOD/SCID leukemogenesis, kinase activity assay

    PMID:18453543

    Open questions at the time
    • Direct vs indirect role in Lyn activation unresolved
  18. 2011 Medium

    Identified a mutually inhibitory ABI1-CaMKIIα interaction via the tSNARE domain and CaMKIIα-mediated S88 phosphorylation as a calcium-gated switch for Rac activation and spine maturation.

    Evidence Co-IP, FRET activity sensors, mutagenesis, neuronal spine morphology

    PMID:22993434

    Open questions at the time
    • Direct biochemical demonstration of S88-driven Rac change limited
  19. 2011 Medium

    Mapped a second Abl-interaction tyrosine (Y398) in the SH3 domain, showing combined Y213/Y398 phosphorylation strengthens Abl binding and stimulates Mena phosphorylation.

    Evidence Mutagenesis, in vitro kinase and binding assays

    PMID:21320496

    Open questions at the time
    • Cellular significance of dual-site phosphorylation not validated in vivo
  20. 2012 Medium

    Defined a conserved axon-guidance role through direct ABI-1/MIG-10 (lamellipodin) binding placing ABI-1/WAVE downstream of MIG-10 in netrin and slit pathways.

    Evidence Yeast two-hybrid, Co-IP, domain mapping, C. elegans epistasis, mammalian lamellipodia assay

    PMID:23028145 PMID:23209429

    Open questions at the time
    • Mammalian in vivo guidance role not directly tested
  21. 2013 High

    Showed ABI1 mediates smooth muscle contraction by linking c-Abl/CAS to N-WASP activation and actin polymerization independent of myosin light chain phosphorylation.

    Evidence Co-IP, lentiviral RNAi, FRET N-WASP sensor, actin polymerization and contraction assays

    PMID:23740246

    Open questions at the time
    • Order of complex assembly during contraction not fully resolved
  22. 2014 Medium

    Connected tyrosine-phosphorylated ABI1 (pY435) to invasion, localizing it to matrix-degradation sites in an Abl-dependent manner.

    Evidence RNAi knockdown, matrix degradation assay, STI571 treatment, immunofluorescence

    PMID:24913355

    Open questions at the time
    • Kinase site specificity for Y435 not biochemically isolated
  23. 2015 Medium

    Defined how ABI1 routes the WAVE2 complex toward lamellipodia vs invadopodia, with a proline-rich/SH3 linker required for c-Abl binding and Abl-mediated WAVE2 phosphorylation.

    Evidence Domain deletion, Co-IP, imatinib/siRNA, morphological assays; tri-complex domain mapping with TAT peptides

    PMID:26428302 PMID:31488087

    Open questions at the time
    • Switch between protrusion types not quantitatively dissected
  24. 2018 High

    Established ABI1 as a tumor-suppressive negative regulator of hematopoietic SFK/STAT3/NF-κB signaling, with bone-marrow KO producing a myeloproliferative/myelofibrosis-like disease.

    Evidence Conditional KO mouse, bone marrow transplantation, kinase and signaling assays, patient sample corroboration

    PMID:30213875

    Open questions at the time
    • Direct molecular target through which ABI1 restrains SFKs unclear
  25. 2020 High

    Identified PTEN as the phosphatase that dephosphorylates ABI1 Y213/S216 to trigger calpain-dependent degradation, downregulating the WAVE complex and driving epithelial polarization and, when lost, EMT.

    Evidence Phospho-IP/MS, PTEN mutant reconstitution, calpain inhibitor rescue, embryoid body and breast cancer EMT assays

    PMID:32673396 PMID:32728066

    Open questions at the time
    • Calpain cleavage site on ABI1 not mapped
  26. 2020 Medium

    Refined leading-edge mechanics, showing ABI1 recruits profilin-1, c-Abl and N-WASP to the lamellipodial tip under integrin β1/c-Abl control, and that CRISPR KO blocks Bcr-Abl ERK/PI3K signaling and leukemogenesis.

    Evidence shRNA/CRISPR KO, live imaging, Co-IP, leading-edge localization, in vivo leukemogenesis

    PMID:32276588 PMID:32606387

    Open questions at the time
    • Direct profilin-1 binding interface not mapped
  27. 2024 Medium

    Identified ubiquitin-mediated turnover via CBLC that degrades ABI1 to activate ERK signaling in colorectal cancer, complementing calpain proteolysis as a degradation route.

    Evidence Ubiquitination assay, Co-IP, gain/loss-of-function, ERK analysis, xenograft

    PMID:38743987

    Open questions at the time
    • Ubiquitination site(s) on ABI1 not defined
  28. 2026 High

    Resolved a spectrin-tethered reservoir mechanism whereby pathogen-induced calpain cleavage releases cortical ABI1 to bind EPS8 and activate its actin-capping activity for protrusion-based cell-to-cell spread.

    Evidence Conditional KO mouse, Co-IP, calpain inhibition, Ca2+ imaging, protrusion/spread assay during Listeria infection

    PMID:41974702

    Open questions at the time
    • Generality of spectrin-tethered ABI1 pool beyond infection unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • How ABI1's many phosphorylation sites, splice isoforms, and competing proteolysis routes are integrated to select between WAVE-driven protrusion, N-WASP-driven trafficking, nuclear transcription, and kinase regulation in a given cell remains unresolved.
  • No structural model of full-length ABI1 in its complexes
  • Quantitative rules governing isoform/PTM-dependent partner choice undefined
  • In vivo coordination of cytoplasmic vs nuclear ABI1 pools unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 6 GO:0008092 cytoskeletal protein binding 4 GO:0098772 molecular function regulator activity 4 GO:0140110 transcription regulator activity 1
Localization
GO:0005886 plasma membrane 4 GO:0005829 cytosol 3 GO:0005856 cytoskeleton 3 GO:0005634 nucleus 1
Pathway
R-HSA-1643685 Disease 5 R-HSA-1266738 Developmental Biology 4 R-HSA-162582 Signal Transduction 4 R-HSA-5653656 Vesicle-mediated transport 4
Partners
ABL1EPS8ITGA4PIK3R1SHANK3SOS1WASLWAVE2
Complex memberships
EPS8-ABI1-SOS1 tri-complexWAVE regulatory complex (WAVE2-ABI1-NAP1-PIR121)

Evidence

Reading pass · 51 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 E3B1/ABI1 was identified as a binding partner of the EPS8 SH3 domain and the ABL SH3 domain in vitro, and associates with EPS8 in vivo; the protein exists as multiple phosphoserine-containing isoforms (65, 68, 72 kDa) whose hyperphosphorylation is induced by EGF stimulation. SH3-domain library screen, in vitro binding assay, in vivo co-immunoprecipitation, phosphatase digestion and phosphoamino acid analysis Oncogene Medium 9010225
1999 EPS8 and E3B1/ABI1 transduce signals from Ras to Rac by forming a tri-complex with SOS-1 that exhibits Rac-specific GEF activity in vitro, placing ABI1 as an essential scaffold in the Ras→Rac signaling axis. In vivo co-immunoprecipitation, in vitro Rac-GEF activity assay, epistatic signaling analysis Nature High 10499589
1998 ABI1 (human ABI-1) was identified as a fusion partner of MLL in acute myeloid leukemia with t(10;11)(p11.2;q23); the MLL-ABI1 fusion combines MLL AT-hook/DNA methyltransferase homology domains with ABI1's homeodomain homology region, polyproline stretches, and SH3 domain. RT-PCR cloning of chimeric transcripts, chromosomal localization, sequence analysis Blood Medium 9694699
2000 Abi-1 and Abi-2 proteins are expressed in the developing nervous system, are concentrated in synaptosomes and growth cone particles, and their phosphorylation state changes with age in brain lysates, consistent with participation in Abl kinase signaling at neuronal structures. Subcellular fractionation, immunohistochemistry, Western blotting of brain lysates Molecular and cellular neurosciences Low 10995551
2000 ABI-1 SH3 domain interacts with ENL (an MLL fusion partner) via an internal ENL region; the interaction was confirmed by yeast two-hybrid, far-Western blot, GST pulldown, and co-immunoprecipitation. Yeast two-hybrid, far-Western blot, GST pulldown, co-immunoprecipitation Oncogene Medium 10777208
2003 PI3K (via its p85 regulatory subunit) is recruited by ABI1 into the EPS8-ABI1-SOS1 complex; PIP3 (the PI3K catalytic product) and p85 recruitment cooperate to unmask the Rac-GEF activity of the complex in vitro and are required for Rac activation and actin remodeling in vivo. Co-immunoprecipitation, in vitro Rac-GEF assay, dominant-negative and KO cell experiments, colocalization studies The Journal of cell biology High 12515821
2004 ABI1 directly interacts with the WHD domain of WAVE2, stimulates WAVE2-mediated actin polymerization activity in vitro, and mediates assembly of the WAVE2-ABI1-NAP1-PIR121 complex; RNAi knockdown of ABI1 abrogates Rac-dependent lamellipodia protrusion in vivo. Tandem mass spectrometry, in vitro actin polymerization assay, co-immunoprecipitation, RNAi knockdown with live-cell imaging Nature cell biology High 15048123
2004 ABI1 binds WAVE1 through an amino-terminal WAVE-binding (WAB) domain; disruption of this interaction prevents ABI1 from reaching lamellipodia tips and reduces WAVE1 protein levels in fibroblasts lacking one Abi1 allele and null for Abi2, an effect rescued by Abi1 constructs retaining WAVE1 binding. ABI1 also contains an overlapping SNARE domain that interacts with Syntaxin-1 and undergoes leptomycin B-sensitive nucleocytoplasmic shuttling. Domain mapping, rescue experiments in MEFs, co-immunoprecipitation, leptomycin B treatment, live-cell localization Molecular and cellular biology Medium 15143189
2005 ABI1 binds N-WASP with nanomolar affinity and, cooperating with Cdc42, potently induces N-WASP actin polymerization activity in vitro. ABI1 and WAVE are both required for Rac-dependent membrane protrusion and macropinocytosis, whereas ABI1 and N-WASP (but not WAVE) regulate actin-based vesicular transport, EGFR endocytosis, and receptor surface distribution. In vitro actin polymerization assay, binding affinity measurements, RNAi knockdown, EGFR endocytosis assay, macropinocytosis assay Nature cell biology High 16155590
2005 Eps8 promotes actin filopodia-like structures; interaction with ABI1 modulates actin architecture toward cables and star-like structures, and the Eps8/ABI1 complex recruits N-WASP to regulate actin nucleator localization/activity. Overexpression in cultured cells and Xenopus embryos, colocalization, dominant-negative constructs BMC cell biology Low 16225669
2005 In response to CSF-1, WAVE2 and ABI1 form a stable complex in macrophages and are co-recruited to F-actin-rich protrusions; knockdown of either WAVE2 or ABI1 by RNAi impairs CSF-1-induced membrane protrusions and macrophage migration. Co-immunoprecipitation, RNAi knockdown, dominant-negative constructs, fluorescence microscopy Journal of cell science Medium 16280551
2006 ABI1 interacts with betaPIX (a Rac1 GEF) via its C-terminal region in vitro and via N-terminal region in vivo; co-expression of ABI1 with truncated betaPIX blocks PDGF-induced dorsal membrane ruffle formation. Yeast two-hybrid, in vitro pulldown, co-immunoprecipitation, overexpression functional assay Cell research Low 16940963
2007 ABI1 is localized in neurites, growth cones, and later enriched in dendritic spines and PSDs. It co-immunoprecipitates with the ProSAP2/Shank3 scaffold and with Eps8/SOS1. Upon NMDA stimulation, ABI1 translocates from PSDs to nuclei in an Abl kinase-dependent manner, where it associates with Myc/Max transcription factor complex and enhances E-box-regulated gene transcription. Knockdown causes excessive dendritic branching and immature spine/synapse morphology. Co-immunoprecipitation, siRNA knockdown, immunofluorescence, nuclear translocation assay, reporter gene assay The EMBO journal Medium 17304222
2007 Chlamydia trachomatis infection promotes Rac interaction with WAVE2 and ABI1 (but not IRSp53) as shown by co-immunoprecipitation; siRNA depletion of WAVE2 or ABI1 abrogates chlamydia-induced actin recruitment and significantly reduces uptake. Co-immunoprecipitation, siRNA knockdown, infection assay Cellular microbiology Medium 17501982
2007 Abi-1 forms an EGF-inducible complex with the Cbl ubiquitin ligase; an Abi-1 mutant lacking the SH3 domain fails to interact with Cbl, inhibits EGFR internalization, and prevents Cbl membrane accumulation after EGF. Oncogenic Abl disrupts the EGF-inducible Abi-1/Cbl complex. Co-immunoprecipitation, dominant-negative overexpression, EGFR internalization assay Cellular signalling Medium 17395426
2008 Phosphotyrosine pY213 of ABI1 allosterically inhibits nonmyristoylated c-Abl kinase in trans by concurrent binding of pY213 to the Abl SH2 domain and a proximal PXXP motif to the Abl SH3 domain; wild-type ABI1 (but not Y213F mutant) inhibits Abl pY412 autophosphorylation in vivo. In vitro kinase assay with phosphopeptides, mutagenesis (Y213F), co-expression in cells, phosphorylation assays Biochimica et biophysica acta High 18328268
2009 In C. elegans, ABI-1 interacts with the UNC-53/NAV2 scaffolding protein via a restricted calponin homology (CH) domain of UNC-53; ABI-1 and UNC-53 share overlapping expression and similar cell-migration phenotypes, and ABI-1 links UNC-53 to the ARP2/3 complex for actin remodeling during cell migration. Yeast two-hybrid, domain mapping, phenotypic analysis of mutants, RNAi of actin-regulatory partners Development Medium 19168673
2010 ABI1 isoform 3 (lacking exon 10) constitutively activates Rac1 and promotes macropinocytosis; isoform 2 (containing exon 10) preferentially binds activated Rac1 via an exon-10 site, suppresses macropinocytosis, and is more cytoplasmic. Both isoforms bind inactive Rac1 through a non-exon-10 site. Flow cytometry macropinocytosis assay, Rac1 activation assay, in vitro binding with exon-10 domain, subcellular fractionation PloS one Medium 20479892
2010 ABI1 pY213 links Abl kinase signaling to the p85 regulatory subunit of PI3K: phosphorylation of Y213 is required for Abl to regulate macropinocytosis, and pY213 interacts with the C-terminal SH2 domain of p85. SH2 domain library screen, interaction mapping, loss-of-function Y213 mutant, macropinocytosis assay FEBS letters Medium 20598684
2010 Dysbindin-1 interacts with WAVE2 (but not N-WASP) and with ABI1, and dysbindin-1 promotes the WAVE2-ABI1 interaction; the three proteins form a ternary complex. RNAi knockdown of dysbindin-1 produces abnormally elongated immature dendritic protrusions, phenocopying ABI1 loss. Co-immunoprecipitation, RNAi knockdown, immunofluorescence Molecular psychiatry Medium 20531346
2010 Integrity of the SOS1/EPS8/ABI1 tri-complex is essential for LPA-induced Rac activation, cell migration, and peritoneal metastatic colonization of ovarian cancer cells; ABI1 acts as the scaffold holding SOS1 and EPS8 together in the complex. Co-immunoprecipitation, knockdown, re-expression rescue, in vivo metastasis assay Cancer research Medium 21118970
2010 Abi1 directly binds the α4 integrin cytoplasmic tail (confirmed by purified protein binding assay), colocalizes with phosphorylated α4 at the leading edge, and Abi1-deficient cells expressing α4 have impaired cell spreading rescued by wild-type but not α4-binding-deficient Abi1. Direct binding assay with purified proteins, co-localization, KO mouse, rescue with Abi1 mutant Proceedings of the National Academy of Sciences High 21173240
2011 Abi1 knockout in mice causes embryonic lethality (~E11.5) with heart and brain malformations. Abi1-KO cells show reduced WAVE1, WAVE2, NAP1, and Sra-1/PIR121 levels, decreased migration rate and distance, and decreased dorsal ruffling, demonstrating that Abi1 is critical for WAVE complex integrity and stability. Conditional KO mouse, Western blotting, cell migration assay, ruffling assay Proceedings of the National Academy of Sciences High 21482783
2011 CDK1/cyclin B phosphorylates ABI1 at serine 216 at mitotic entry, and this phosphorylation attenuates Bcr-Abl-induced tyrosine phosphorylation of the WAVE complex and F-actin assembly; phosphomimetic S216D mutation in ABI1 is sufficient to recapitulate this attenuation and interferes with cell cycle progression. In vitro kinase assay, phosphomimetic mutagenesis, cell cycle analysis, F-actin assembly assay The Journal of biological chemistry High 21900237
2011 Abi1 knockdown by shRNA in Bcr-Abl-transformed cells attenuates abnormal actin remodeling, inhibits cell adhesion and migration on fibronectin, and impairs leukemogenesis in NOD/SCID mice; Abi1 silencing also attenuates Bcr-Abl-stimulated activation of Lyn (Src family kinase). shRNA knockdown, actin remodeling assay, adhesion/migration assay, in vivo leukemogenesis assay, kinase activity assay Carcinogenesis Medium 18453543
2011 Abi-1 and CaMKIIα interact at resting conditions through Abi1's tSNARE domain (which has homology to CaMKIIα regulatory domain); this interaction mutually inhibits both proteins including Abi1-dependent Rac activation. Glutamate receptor activation causes calmodulin binding leading to dissociation; CaMKIIα phosphorylates Abi1 at serine 88 prior to dissociation, contributing to Rac activation and spine maturation. Co-immunoprecipitation, FRET-based activity sensors, mutagenesis, spine morphology analysis in neurons The Journal of neuroscience Medium 22993434
2011 An SK3 channel/nWASP/Abi-1 trimeric complex is present in neural stem cells and postsynaptic compartments; SK3 and nWASP act synergistically to induce filopodial outgrowth, while Abi-1 acts antagonistically to these partners to regulate neurite outgrowth. Co-immunoprecipitation, overexpression, pharmacological SK3 channel activation, fluorescence microscopy PloS one Low 21464958
2011 hnRNPK interacts with the Abi-1 SH3 domain through the hnRNPK KI domain; knockdown of hnRNPK phenocopies Abi-1 knockdown (enlarged dendritic tree, increased filopodia, decreased mature synapses), indicating synergistic function in a multiprotein complex regulating spine/filopodia balance. Co-immunoprecipitation, domain mapping, siRNA knockdown, fluorescence microscopy PloS one Low 22102872
2012 C. elegans ABI-1 SH3 domain directly binds MIG-10 (lamellipodin); epistasis analysis places ABI-1 and WVE-1 downstream of MIG-10 to mediate axon guidance in both UNC-6 (netrin) and SLT-1 (slit) pathways; this ABI-1/MIG-10 interaction also promotes lamellipodia formation in cultured mammalian cells. Yeast two-hybrid, co-immunoprecipitation, domain mapping, epistasis analysis, cell-based lamellipodia assay PLoS genetics Medium 23209429
2012 MIG-10B isoform interacts with ABI-1 to organize the actin cytoskeleton at presynaptic sites and instruct synaptic vesicle clustering through Synapsin-1 (SNN-1) downstream of Netrin; a dominant-negative MIG-10B construct expressing a specific N-terminal domain disrupts vesicle clustering and thermotaxis behavior in vivo. Genetic analysis, domain mapping, dominant-negative construct, in vivo behavioral assay Genes & development Medium 23028145
2013 Stimulation with acetylcholine induces Abi1 association with N-WASP in smooth muscle; Abi1 knockdown inhibits N-WASP activation, actin polymerization, and contraction without affecting myosin light chain phosphorylation. A multiprotein complex of c-Abl, CAS, and Abi1 forms upon contractile activation, and Abi1 is activated by c-Abl/CAS while Abi1 reciprocally controls c-Abl activation (pY412) and CAS interaction. Co-immunoprecipitation, lentiviral RNAi, FRET-based N-WASP sensor, actin polymerization assay, contraction assay The Journal of biological chemistry High 23740246
2014 Y435-phosphorylated ABI1 localizes at sites of extracellular matrix degradation in colorectal carcinoma cells; RNAi knockdown of ABI1 suppresses matrix dissolution by 95%, and STI571 treatment abolishes ABI1 Y435-phosphorylation and suppresses matrix degradation, fibronectin attachment, and cell invasion. RNAi knockdown, matrix degradation assay, STI571 kinase inhibitor treatment, immunofluorescence Molecular cancer Medium 24913355
2010 Activated Rac1 specifically binds to a site encoded by exon 10 in ABI1 isoform 2 in vitro; because exon 10 is absent in isoform 3 due to alternative splicing, the isoform-2 WAVE2 complex and isoform-3 WAVE2 complex display differential binding to activated versus inactive Rac1. In vitro binding assay with activated/inactive Rac1, domain mapping by exon exclusion PloS one Medium 20479892
2015 ABI1 acts as a scaffold protein in the SOS1/EPS8/ABI1 tri-complex: the SH3 domain of ABI1 mediates SOS1 binding, and the poly-proline+PxxDY region mediates EPS8 binding; TAT-conjugated inhibitory peptides disrupting ABI1-EPS8 interaction suppress ovarian cancer invasion and metastasis in vivo. Co-immunoprecipitation, GST pulldown, domain deletion mapping, in vivo peritoneal metastasis assay with inhibitory peptides BMC cancer Medium 31488087
2015 ABI1 bridges c-Abl and WAVE2: a linker region between the proline-rich and SH3 domains of ABI1 is required for c-Abl interaction and c-Abl-mediated WAVE2 phosphorylation; the NESH/Abi-3-based WAVE2 complex, lacking this c-Abl-binding linker, is unable to generate lamellipodial protrusions and instead may promote invadopodia. Domain deletion analysis, co-immunoprecipitation, imatinib/siRNA knockdown, morphological assays Cell communication and signaling Medium 26428302
2018 Bone marrow-specific deletion of Abi1 in mice results in an MPN-like phenotype with increased activity of Src family kinases (SFKs), STAT3, and NF-κB signaling, impaired hematopoietic stem cell self-renewal, and features resembling human myelofibrosis; ABI1 thus negatively regulates SFK/STAT3/NF-κB signaling in hematopoietic cells. Conditional KO mouse, bone marrow transplantation, kinase activity assays, signaling pathway analysis Blood High 30213875
2019 ABI1 loss in benign prostate epithelial cells induces EMT and activation of non-canonical WNT signaling; FYN SH2 domain interacts with ABI1 pY421 (high-affinity), and ABI1 loss leads to increased STAT3 phosphorylation downstream of FZD2, suggesting ABI1 inhibits EMT through suppressing FYN-STAT3 activation. CRISPR/Cas9 gene disruption, RNA sequencing, Western blotting, SH2-ABI1 interaction assay Cell communication and signaling Medium 31530281
2020 PTEN dephosphorylates ABI1 at Y213 and S216 (identified by phosphotyrosine immunoprecipitation/MS), leading to ABI1 degradation through the calpain pathway; this down-regulates the WAVE regulatory complex, reorganizes the actin cytoskeleton, and is required for epiblast epithelial polarization. Phosphotyrosine IP/mass spectrometry, PTEN mutant reconstitution in embryoid bodies, calpain inhibitor rescue, embryoid body polarization assay The Journal of cell biology High 32673396
2020 PTEN's protein phosphatase activity dephosphorylates and downregulates ABI1 in breast cancer cells; gain- and loss-of-function of ABI1 demonstrates that ABI1 mediates PTEN-loss-induced EMT and cancer stem cell activity. PTEN reconstitution (WT vs. mutant), gain/loss-of-function of ABI1, EMT marker analysis Scientific reports Medium 32728066
2020 ABI1 localizes to the tip of lamellipodia and its protrusion coordinates with F-actin at the leading edge; ABI1 knockdown reduces smooth muscle cell migration and decreases leading-edge recruitment of profilin-1 (Pfn-1, identified as a new ABI1 partner), c-Abl, and N-WASP but not pVASP, cortactin, or vinculin. Integrin β1 and c-Abl regulate ABI1 positioning at the leading edge. shRNA knockdown/rescue, live-cell imaging, co-immunoprecipitation, leading-edge protein localization assay Scientific reports Medium 32606387
2011 Y398 in the SH3 domain of Abi-1 is a new phosphorylation site; combined disruption of Y213 and Y398 significantly weakens Abi-1 binding to c-Abl (SH3-Abl proline-rich domain interaction); Abi-1 phosphorylation at both sites stimulates Mena phosphorylation through c-Abl kinase activation. Mutagenesis, in vitro kinase assay, binding assay, phosphorylation assay FEBS letters Medium 21320496
2004 E3B1/ABI1 levels are regulated by the Ca2+-activated protease calpain; serum starvation activates calpain-dependent E3B1 degradation, which is prevented by constitutively active PAK1 or by Rac1 activation downstream of PDGF; conversely, PAK auto-inhibitory domain expression causes E3B1 degradation even in serum. Calpain inhibitor treatment, constitutively active/dominant-negative constructs, Western blotting Biochemical and biophysical research communications Low 15178460
2012 Abi1 mediates airway smooth muscle (ASM) cell proliferation by regulating JAK2 and STAT3 activation; Abi1 knockdown reduces JAK2 and STAT3 activation and ASM proliferation. shRNA loss-of-function, kinase/STAT3 phosphorylation assay, proliferation assay iScience Medium 35198891
2020 CRISPR/Cas9 knockout of Abi1 in Bcr-Abl-transformed hematopoietic cells reduces ERK and PI3K/Akt signaling, inhibits IL3-independent growth and SDF-1α-mediated chemotaxis, abrogates abnormal actin remodeling, and abolishes leukemogenesis in syngeneic mice. CRISPR/Cas9 KO, signaling pathway analysis, in vivo leukemogenesis assay Journal of hematology & oncology Medium 32276588
2013 Xenopus Abi1 knockdown by morpholino disrupts eye morphogenesis; the SH3 domain of Abi1 is required for proper retinal progenitor cell movement and differentiation, and this requires an interaction with WASP (Wiskott-Aldrich syndrome protein). Morpholino knockdown, SH3 domain rescue constructs, co-immunoprecipitation The Journal of biological chemistry Medium 23558677
2021 Loss of MBNL1 promotes expression of the Abi1-Δe10 splice isoform (lacking exon 10); Abi1-Δe10 activates Rac1 independently of upstream stimulation and triggers the Rac1-NOX1-ROS pathway leading to KLF4 upregulation, VSMC macrophage-like transdifferentiation, increased proliferation and migration. MBNL1 knockdown, splice isoform expression analysis, Rac1 activation assay, NOX1-ROS pathway analysis, VSMC phenotyping Cell proliferation Medium 33759281
2024 Caskin2 associates with the WAVE regulatory complex in an ABI1-dependent manner to promote cell migration; growth factor-induced phosphorylation of Caskin2 at serine 878 regulates the Caskin2-ABI1 interaction. Co-immunoprecipitation, phospho-mutant analysis, cell migration assay Journal of cell science Low 38587458
2026 During Listeria monocytogenes infection, the pore-forming toxin LLO triggers Ca2+ influx leading to calpain-mediated spectrin cleavage; this mobilizes ABI1 from its spectrin-bound cortical state, allowing ABI1 to bind EPS8 within protrusions and activate EPS8's actin-capping activity to enable actin recycling and protrusion elongation for cell-to-cell spread. Conditional Abi1 KO mice show significantly reduced Lm infection susceptibility. Conditional KO mouse, co-immunoprecipitation, calpain inhibitor assay, Ca2+ imaging, protrusion/spread assay Nature communications High 41974702
2024 ABI1 is imported to the plant cell nucleus by SAD2 (an importin β family protein) upon coronatine (COR) treatment; nuclear ABI1 accumulation requires SAD2 and enables COR to antagonize ABA signaling and reopen stomata during bacterial pathogen invasion. Subcellular localization assay, sad2 mutant analysis, COR treatment, stomatal aperture assay The Plant journal Medium 38683723
2020 CaMKIIα phosphorylates Shank3 at Ser685; this phosphorylation significantly increases binding of ABI1 to Shank3 in vitro (pre-phosphorylated GST-Shank3), whereas Ser685Ala or Ser685Asp mutation reduces ABI1-Shank3 co-immunoprecipitation from cells. In vitro kinase assay with purified CaMKIIα, co-immunoprecipitation, mutagenesis Biochemical and biophysical research communications Medium 31983435
2024 ABI1 degradation through ubiquitination by the E3 ligase CBLC activates ERK signaling in colorectal cancer; ABI1 upregulation abolishes the pro-tumorigenic effects of CBLC. Ubiquitination assay, co-immunoprecipitation, gain/loss-of-function, ERK pathway analysis, xenograft assay Translational oncology Medium 38743987

Source papers

Stage 0 corpus · 98 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway. The Plant journal : for cell and molecular biology 449 11208021
1999 ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling. The Plant cell 432 10521520
2001 Abscisic acid activation of plasma membrane Ca(2+) channels in guard cells requires cytosolic NAD(P)H and is differentially disrupted upstream and downstream of reactive oxygen species production in abi1-1 and abi2-1 protein phosphatase 2C mutants. The Plant cell 382 11701885
2004 Phospholipase D alpha 1-derived phosphatidic acid interacts with ABI1 phosphatase 2C and regulates abscisic acid signaling. Proceedings of the National Academy of Sciences of the United States of America 372 15197253
2012 Reconstitution of abscisic acid activation of SLAC1 anion channel by CPK6 and OST1 kinases and branched ABI1 PP2C phosphatase action. Proceedings of the National Academy of Sciences of the United States of America 341 22689970
2004 Abi1 is essential for the formation and activation of a WAVE2 signalling complex. Nature cell biology 341 15048123
1999 EPS8 and E3B1 transduce signals from Ras to Rac. Nature 290 10499589
2015 Degradation of the ABA co-receptor ABI1 by PUB12/13 U-box E3 ligases. Nature communications 258 26482222
2003 Phosphoinositide 3-kinase activates Rac by entering in a complex with Eps8, Abi1, and Sos-1. The Journal of cell biology 215 12515821
2005 Abi1 regulates the activity of N-WASP and WAVE in distinct actin-based processes. Nature cell biology 182 16155590
2004 Drought tolerance established by enhanced expression of the CC-NBS-LRR gene, ADR1, requires salicylic acid, EDS1 and ABI1. The Plant journal : for cell and molecular biology 168 15144382
2017 Abscisic Acid Signaling Inhibits Brassinosteroid Signaling through Dampening the Dephosphorylation of BIN2 by ABI1 and ABI2. Molecular plant 135 29275167
2007 Abelson interacting protein 1 (Abi-1) is essential for dendrite morphogenesis and synapse formation. The EMBO journal 100 17304222
1997 Isolation and characterization of e3B1, an eps8 binding protein that regulates cell growth. Oncogene 89 9010225
2013 Type 2C protein phosphatase ABI1 is a negative regulator of strawberry fruit ripening. Journal of experimental botany 86 23404898
2010 Dysbindin-1, WAVE2 and Abi-1 form a complex that regulates dendritic spine formation. Molecular psychiatry 81 20531346
2010 Integrity of SOS1/EPS8/ABI1 tri-complex determines ovarian cancer metastasis. Cancer research 79 21118970
2004 Abl interactor 1 (Abi-1) wave-binding and SNARE domains regulate its nucleocytoplasmic shuttling, lamellipodium localization, and wave-1 levels. Molecular and cellular biology 77 15143189
1997 Alteration of anion channel kinetics in wild-type and abi1-1 transgenic Nicotiana benthamiana guard cells by abscisic acid. The Plant journal : for cell and molecular biology 74 9263461
2005 WASP-related proteins, Abi1 and Ena/VASP are required for Listeria invasion induced by the Met receptor. Journal of cell science 70 15769844
2007 ABA is required for Leptosphaeria maculans resistance via ABI1- and ABI4-dependent signaling. Molecular plant-microbe interactions : MPMI 67 17427804
2005 A WAVE2-Abi1 complex mediates CSF-1-induced F-actin-rich membrane protrusions and migration in macrophages. Journal of cell science 66 16280551
2011 Essential role for Abi1 in embryonic survival and WAVE2 complex integrity. Proceedings of the National Academy of Sciences of the United States of America 63 21482783
2000 Localization and phosphorylation of Abl-interactor proteins, Abi-1 and Abi-2, in the developing nervous system. Molecular and cellular neurosciences 63 10995551
1998 ABI-1, a human homolog to mouse Abl-interactor 1, fuses the MLL gene in acute myeloid leukemia with t(10;11)(p11.2;q23). Blood 63 9694699
2019 ABA signaling is negatively regulated by GbWRKY1 through JAZ1 and ABI1 to affect salt and drought tolerance. Plant cell reports 62 31713664
2007 Rac interacts with Abi-1 and WAVE2 to promote an Arp2/3-dependent actin recruitment during chlamydial invasion. Cellular microbiology 59 17501982
2009 The cell migration molecule UNC-53/NAV2 is linked to the ARP2/3 complex by ABI-1. Development (Cambridge, England) 58 19168673
2013 Role of the adapter protein Abi1 in actin-associated signaling and smooth muscle contraction. The Journal of biological chemistry 53 23740246
2003 The abi1-1 mutation blocks ABA signaling downstream of cADPR action. The Plant journal : for cell and molecular biology 51 12713537
2011 An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis. PloS one 43 21464958
2001 Functional interactions of lanthanum and phospholipase D with the abscisic acid signaling effectors VP1 and ABI1-1 in rice protoplasts. The Journal of biological chemistry 41 11139577
2017 The sHSP22 Heat Shock Protein Requires the ABI1 Protein Phosphatase to Modulate Polar Auxin Transport and Downstream Responses. Plant physiology 40 29288233
2005 Regulation of actin cytoskeleton architecture by Eps8 and Abi1. BMC cell biology 40 16225669
2016 Protein phosphatase type 2C PP2CA together with ABI1 inhibits SnRK2.4 activity and regulates plant responses to salinity. Plant signaling & behavior 39 27901636
2010 Abl-interactor-1 (Abi1) has a role in cardiovascular and placental development and is a binding partner of the alpha4 integrin. Proceedings of the National Academy of Sciences of the United States of America 39 21173240
2005 High frequencies of Y chromosome lineages characterized by E3b1, DYS19-11, DYS392-12 in Somali males. European journal of human genetics : EJHG 39 15756297
2019 Abi1 loss drives prostate tumorigenesis through activation of EMT and non-canonical WNT signaling. Cell communication and signaling : CCS 38 31530281
2012 Abi1, a critical molecule coordinating actin cytoskeleton reorganization with PI-3 kinase and growth signaling. FEBS letters 37 22617151
2012 Synaptic vesicle clustering requires a distinct MIG-10/Lamellipodin isoform and ABI-1 downstream from Netrin. Genes & development 36 23028145
2000 The interaction between EEN and Abi-1, two MLL fusion partners, and synaptojanin and dynamin: implications for leukaemogenesis. Leukemia 36 10764144
2010 Differential regulation of macropinocytosis by Abi1/Hssh3bp1 isoforms. PloS one 35 20479892
2008 Abi1 gene silencing by short hairpin RNA impairs Bcr-Abl-induced cell adhesion and migration in vitro and leukemogenesis in vivo. Carcinogenesis 35 18453543
2007 The WAVE2/Abi1 complex differentially regulates megakaryocyte development and spreading: implications for platelet biogenesis and spreading machinery. Blood 35 17664349
2020 PTEN suppresses epithelial-mesenchymal transition and cancer stem cell activity by downregulating Abi1. Scientific reports 34 32728066
2016 Phosphatase ABI1 and okadaic acid-sensitive phosphoprotein phosphatases inhibit salt stress-activated SnRK2.4 kinase. BMC plant biology 34 27297076
2011 Heterogeneous nuclear ribonucleoprotein k interacts with Abi-1 at postsynaptic sites and modulates dendritic spine morphology. PloS one 32 22102872
2018 MiR-181a/b induce the growth, invasion, and metastasis of neuroblastoma cells through targeting ABI1. Molecular carcinogenesis 31 29802737
2014 Expression and Y435-phosphorylation of Abelson interactor 1 (Abi1) promotes tumour cell adhesion, extracellular matrix degradation and invasion by colorectal carcinoma cells. Molecular cancer 31 24913355
2002 In search of a function for the E3B1/Abi2/Argbp1/NESH family (Review). International journal of molecular medicine 31 12011975
2000 Isolation and characterization of a novel human gene (NESH) which encodes a putative signaling molecule similar to e3B1 protein. Biochimica et biophysica acta 31 10978530
2018 Bone marrow-specific loss of ABI1 induces myeloproliferative neoplasm with features resembling human myelofibrosis. Blood 29 30213875
2012 Crk and ABI1: binary molecular switches that regulate abl tyrosine kinase and signaling to the cytoskeleton. Genes & cancer 26 23226578
2010 Abi1/Hssh3bp1 pY213 links Abl kinase signaling to p85 regulatory subunit of PI-3 kinase in regulation of macropinocytosis in LNCaP cells. FEBS letters 26 20598684
2008 Allosteric inhibition of the nonMyristoylated c-Abl tyrosine kinase by phosphopeptides derived from Abi1/Hssh3bp1. Biochimica et biophysica acta 26 18328268
2015 The NESH/Abi-3-based WAVE2 complex is functionally distinct from the Abi-1-based WAVE2 complex. Cell communication and signaling : CCS 25 26428302
2000 ENL, the MLL fusion partner in t(11;19), binds to the c-Abl interactor protein 1 (ABI1) that is fused to MLL in t(10;11)+. Oncogene 25 10777208
2001 t(10;11)-acute leukemias with MLL-AF10 and MLL-ABI1 chimeric transcripts: specific expression patterns of ABI1 gene in leukemia and solid tumor cell lines. Genes, chromosomes & cancer 24 11477655
2001 Inhibition of v-Abl transformation in 3T3 cells overexpressing different forms of the Abelson interactor protein Abi-1. Oncogene 23 11526477
2015 Reciprocal regulation of Abl kinase by Crk Y251 and Abi1 controls invasive phenotypes in glioblastoma. Oncotarget 22 26473374
2022 Abi1 mediates airway smooth muscle cell proliferation and airway remodeling via Jak2/STAT3 signaling. iScience 21 35198891
2021 RNA Splicing of the Abi1 Gene by MBNL1 contributes to macrophage-like phenotype modulation of vascular smooth muscle cell during atherogenesis. Cell proliferation 21 33759281
2012 Disruption of Abi1/Hssh3bp1 expression induces prostatic intraepithelial neoplasia in the conditional Abi1/Hssh3bp1 KO mice. Oncogenesis 21 23552839
2000 Trivalent ions activate abscisic acid-inducible promoters through an ABI1-dependent pathway in rice protoplasts. Plant physiology 21 10938371
2007 Abi-1 forms an epidermal growth factor-inducible complex with Cbl: role in receptor endocytosis. Cellular signalling 20 17395426
2012 MIG-10 functions with ABI-1 to mediate the UNC-6 and SLT-1 axon guidance signaling pathways. PLoS genetics 19 23209429
2015 Involvement of genes encoding ABI1 protein phosphatases in the response of Brassica napus L. to drought stress. Plant molecular biology 18 26059040
2020 Distinctive roles of Abi1 in regulating actin-associated proteins during human smooth muscle cell migration. Scientific reports 17 32606387
2012 Expression of Abelson interactor 1 (Abi1) correlates with inflammation, KRAS mutation and adenomatous change during colonic carcinogenesis. PloS one 17 22808230
2012 Activity-dependent modulation of the interaction between CaMKIIα and Abi1 and its involvement in spine maturation. The Journal of neuroscience : the official journal of the Society for Neuroscience 17 22993434
2020 PTEN dephosphorylates Abi1 to promote epithelial morphogenesis. The Journal of cell biology 16 32673396
2012 Abelson interactor-1 (ABI-1) interacts with MRL adaptor protein MIG-10 and is required in guided cell migrations and process outgrowth in C. elegans. Developmental biology 16 23022657
2006 A new interaction between Abi-1 and betaPIX involved in PDGF-activated actin cytoskeleton reorganisation. Cell research 16 16940963
2019 Inhibitory short peptides targeting EPS8/ABI1/SOS1 tri-complex suppress invasion and metastasis of ovarian cancer cells. BMC cancer 15 31488087
2025 SnRK2 kinases sense molecular crowding and form condensates to disrupt ABI1 inhibition. Science advances 14 39879308
2020 CRISPR/CAS9-mediated knockout of Abi1 inhibits p185Bcr-Abl-induced leukemogenesis and signal transduction to ERK and PI3K/Akt pathways. Journal of hematology & oncology 13 32276588
2011 CDK1-mediated phosphorylation of Abi1 attenuates Bcr-Abl-induced F-actin assembly and tyrosine phosphorylation of WAVE complex during mitosis. The Journal of biological chemistry 13 21900237
2011 Identification and functional analysis of a new phosphorylation site (Y398) in the SH3 domain of Abi-1. FEBS letters 11 21320496
2002 Is t(10;11)(p11.2;q23) involving MLL and ABI-1 genes associated with congenital acute monocytic leukemia? Cancer genetics and cytogenetics 11 12547160
2024 Coronatine orchestrates ABI1-mediated stomatal opening to facilitate bacterial pathogen infection through importin β protein SAD2. The Plant journal : for cell and molecular biology 10 38683723
2022 ABI1-based expression signature predicts breast cancer metastasis and survival. Molecular oncology 10 34967509
2013 Negative regulation of abscisic acid signaling by the Brassica oleracea ABI1 ortholog. Biochemical and biophysical research communications 10 24269821
1998 The role of ABI1 in abscisic acid signal transduction: from gene to cell. Symposia of the Society for Experimental Biology 10 10645425
2012 E3B1/ABI-1 isoforms are down-regulated in cancers of human gastrointestinal tract. Disease markers 9 22430194
2005 E3B1, a human homologue of the mouse gene product Abi-1, sensitizes activation of Rap1 in response to epidermal growth factor. Experimental cell research 9 16182283
2020 CaMKIIα phosphorylation of Shank3 modulates ABI1-Shank3 interaction. Biochemical and biophysical research communications 7 31983435
2024 Caskin2 is a novel talin- and Abi1-binding protein that promotes cell motility. Journal of cell science 4 38587458
2024 ABI3 regulates ABI1 function to control cell length in primary root elongation zone. The Plant journal : for cell and molecular biology 4 39495594
2013 [Abelson interactor 1 (Abi1) in colorectal cancer. From synaptic plasticity to tumor cell migration]. Der Pathologe 4 24196611
2013 Abelson interactor 1 (ABI1) and its interaction with Wiskott-Aldrich syndrome protein (wasp) are critical for proper eye formation in Xenopus embryos. The Journal of biological chemistry 3 23558677
2005 [The role of EGF receptor-dependent e3B1/Abi1 protein as a tumor suppressor protein in malignant tumors]. Orvosi hetilap 3 16025998
2004 Pak regulates calpain-dependent degradation of E3b1. Biochemical and biophysical research communications 3 15178460
2025 Ethylene antagonizes ABA responses in stomatal movement and seed germination by upregulating ABI1 and ABI2 at both the transcript and protein levels. The Plant journal : for cell and molecular biology 2 40811817
2024 Circulating miR-30e-3p induces disruption of neurite development in SH-SY5Y cells by targeting ABI1, a novel biomarker for schizophrenia. Journal of psychiatric research 2 38626565
2026 AlphaFold2 Insights into c-Abl Transactivation by Adaptor c-Crk or Abi-1: Structural Forms, Active Site Types, and Multiple SH3-PxxPxK/R Interactions. Genes to cells : devoted to molecular & cellular mechanisms 0 41618774
2026 A pore-forming toxin initiates ABI1 complex switching to promote bacterial cell-to-cell spread. Nature communications 0 41974702
2024 CBLC promotes the development of colorectal cancer by promoting ABI1 degradation to activate the ERK signaling pathway. Translational oncology 0 38743987
2024 Programmed cell death-related ABI1 is a critical mediator of abdominal aortic aneurysm. European journal of medical research 0 39568082

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