Affinage

ZW10

Centromere/kinetochore protein zw10 homolog · UniProt O43264

Length
779 aa
Mass
88.8 kDa
Annotated
2026-06-11
35 papers in source corpus 21 papers cited in narrative 21 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ZW10 is a dual-function protein that operates at the kinetochore during mitosis and at the endomembrane system during interphase, in both contexts coupling cargo to the dynein/dynactin motor (PMID:1339459, PMID:9700164, PMID:16505164). In dividing cells ZW10 shows cell cycle-dependent kinetochore localization and is required for accurate chromosome segregation, with loss causing lagging chromosomes and attenuated poleward chromosome motion (PMID:1339459, PMID:11146661). It assembles with ROD and Zwilch into the evolutionarily conserved RZZ complex—a large (~700–900 kDa) particle containing two copies of each subunit—whose members mutually depend on one another for recruitment to the mitotic apparatus (PMID:11590237, PMID:12686595, PMID:25849506). RZZ is recruited to kinetochores through a sequential Hec1→Zwint-1→ZW10 hierarchy, and ZW10 in turn recruits the dynein/dynactin motor (binding the dynactin subunit dynamitin through its N-terminal domain) and enables stable Mad1–Mad2 loading at unattached kinetochores, thereby bridging structural kinetochore components to the spindle assembly checkpoint (PMID:9700164, PMID:15824131, PMID:16732327, PMID:18782227). The same N-terminal region binds dynamitin, RINT-1, and Zwint-1 in a mutually exclusive manner, so that partner switching at this domain governs ZW10 localization and dynein linkage (PMID:18782227). PLK1 phosphorylates ZW10 at Ser12 to dynamically tune the ZW10–Zwint-1 interaction and fine-tune checkpoint silencing and segregation fidelity (PMID:38402459). During interphase ZW10 relocalizes to the ER, cytosol, and Golgi/endosomal membranes, where it joins a RINT-1/p31 subcomplex associated with the syntaxin 18 ER t-SNARE to mediate retrograde ER–Golgi membrane trafficking and recruits dynein to membranes for minus-end-directed organelle movement (PMID:15029241, PMID:16505164, PMID:16571679).

Mechanistic history

Synthesis pass · year-by-year structured walk · 21 steps
  1. 1992 High

    Established ZW10 as a cell cycle-regulated factor whose proper behavior is needed for faithful chromosome segregation, framing it as an anaphase-onset effector rather than a static structural protein.

    Evidence Immunofluorescence tracking of dynamic localization and mutant phenotype analysis in Drosophila neuroblasts

    PMID:1339459

    Open questions at the time
    • Molecular partners and biochemical activity unknown
    • The filamentous structure ZW10 associates with at metaphase not molecularly defined
  2. 1994 High

    Placed ZW10 within a multicomponent kinetochore pathway by showing its kinetochore localization depends on rough deal and abnormal anaphase resolution gene products, indicating ZW10 acts as part of a complex rather than alone.

    Evidence Genetic epistasis and immunofluorescence in Drosophila mutant backgrounds

    PMID:7914521

    Open questions at the time
    • Physical nature of the interactions not yet shown
    • Downstream effectors of the pathway unidentified
  3. 1996 High

    Linked ZW10 kinetochore residency to spindle attachment/tension, showing its localization predicts chromosome fate and positioning it within a tension-sensing mechanism for anaphase control.

    Evidence Immunofluorescence correlated with chromosome movement in Drosophila meiotic cells

    PMID:8794856

    Open questions at the time
    • Mechanism translating attachment status into ZW10 redistribution unknown
  4. 1997 High

    Demonstrated evolutionary conservation of ZW10 structure and function from flies to humans and worms, validating model-organism findings for the human protein.

    Evidence Cross-species sequence analysis, anti-human ZW10 IF in HeLa, C. elegans antisense phenocopy

    PMID:9298984

    Open questions at the time
    • Conserved binding partners not yet defined
  5. 1998 High

    Identified the molecular output of kinetochore ZW10 as dynein/dynactin recruitment via a direct dynamitin interaction, explaining its role in anaphase chromosome motion separate from microtubule capture.

    Evidence Yeast two-hybrid (ZW10–dynamitin) and IF in zw10 mutants in Drosophila

    PMID:9700164

    Open questions at the time
    • Dynamitin-binding region of ZW10 initially mismapped (later revised)
    • How dynein recruitment relates to checkpoint not addressed
  6. 1998 Medium

    Distinguished ZW10/Rod from other checkpoint arms by showing Bub3 kinetochore loading is ZW10-independent, revealing parallel kinetochore assembly pathways.

    Evidence Immunofluorescence in Drosophila zw10 and rod mutant neuroblasts

    PMID:9914369

    Open questions at the time
    • Single study with a negative result
    • Relationship to Mad1/Mad2 arm not tested here
  7. 2000 High

    Quantified the functional consequence of ZW10/Rod loss as slowed, asynchronous poleward chromosome motion, tying the complex to the kinetics of segregation.

    Evidence Live imaging of chromosome movement in zw10-null Drosophila cells

    PMID:11146661

    Open questions at the time
    • Direct molecular cause of motion defect (dynein vs other) not isolated in this assay
  8. 2001 High

    Defined ROD and ZW10 as a single large conserved macromolecular complex that functions in one pathway, consolidating prior genetic interactions into biochemistry.

    Evidence Reciprocal co-IP, size-exclusion chromatography and double-null epistasis in Drosophila and HeLa

    PMID:11590237

    Open questions at the time
    • Complete subunit composition not yet known
    • Stoichiometry undefined
  9. 2003 High

    Completed the core complex by identifying Zwilch as the third subunit, defining the trimeric RZZ complex and a candidate kinesin (CENP-meta) interaction.

    Evidence Immunoaffinity chromatography/MS and co-IP from HeLa, Drosophila mutant analysis

    PMID:12686595

    Open questions at the time
    • CENP-meta interaction weak and unvalidated functionally
    • Subunit stoichiometry not resolved
  10. 2004 High

    Revealed a distinct interphase function, placing ZW10 in a RINT-1/p31/syntaxin 18 SNARE assembly that mediates ER–Golgi membrane trafficking.

    Evidence Co-IP, fractionation, overexpression, antibody microinjection, siRNA and Golgi morphology assays

    PMID:15029241

    Open questions at the time
    • How ZW10 partitions between mitotic and interphase complexes unknown
    • Direct binding within the SNARE assembly not mapped
  11. 2005 High

    Established RZZ as the bridge between structural kinetochore components and the checkpoint, showing ZW10 is recruited via Zwint-1 and is required for stable Mad1–Mad2 binding at unattached kinetochores.

    Evidence Co-IP from HeLa mitotic extracts, siRNA depletion and Xenopus egg extract immunodepletion

    PMID:15824131

    Open questions at the time
    • Mechanism by which RZZ promotes Mad1–Mad2 loading not molecularly defined
  12. 2006 High

    Defined the kinetochore recruitment hierarchy Hec1→Zwint-1→ZW10, providing the ordered assembly logic for RZZ-dependent checkpoint control in human cells.

    Evidence M-phase co-IP and sequential siRNA depletion of Hec1 and Zwint-1 in human cells

    PMID:16732327

    Open questions at the time
    • Structural basis of the Zwint-1–ZW10 contact not resolved
  13. 2006 High

    Extended the interphase role to organelle positioning, showing ZW10 loads dynein onto Golgi/endosome/lysosome membranes to drive minus-end-directed organelle movement.

    Evidence Cosedimentation, dominant-negative, antibody injection, RNAi, live imaging and dynein membrane pulldown

    PMID:16505164

    Open questions at the time
    • Whether membrane dynein recruitment uses the same dynamitin contact as at kinetochores not directly shown here
  14. 2006 High

    Ordered the interphase pathway by placing RINT-1 upstream of ZW10 in controlling ZW10 entry into the syntaxin 18 SNARE complex and ER–Golgi transport.

    Evidence Asymmetric knockdown epistasis, co-IP and Golgi morphology assays

    PMID:16571679

    Open questions at the time
    • Single-lab directional epistasis
    • Trigger that recruits ZW10 to RINT-1 unknown
  15. 2008 Medium

    Remapped the dynamitin-binding site to the ZW10 N-terminus and revealed mutually exclusive binding of dynamitin, RINT-1 and Zwint-1, establishing partner switching at one domain as the switch between functions.

    Evidence Yeast two-hybrid, truncation co-IP, competitive binding and live imaging of ZW10 movement

    PMID:18782227

    Open questions at the time
    • Single lab
    • Structural basis of competitive binding not solved
    • Regulation of partner choice in cells not defined
  16. 2008 High

    Dissected RZZ versus dynein contributions at kinetochores, showing RZZ inhibits load-bearing attachments while dynein (recruited via SPDL-1) counteracts this, refining how the complex regulates attachment maturation.

    Evidence RNAi epistasis with kinetochore tension assays and co-IP in C. elegans embryos

    PMID:18765790

    Open questions at the time
    • Molecular mechanism of RZZ attachment inhibition unknown
    • Relationship of SPDL-1 to human Spindly-equivalent in this dataset not established
  17. 2010 Medium

    Connected the Rod/Zw10 complex to centromeric SUMOylation by showing it recruits the SUMO E3 ligase PIASy and supports SUMO2/3 modification at centromeres during mitosis.

    Evidence Co-IP, truncation mapping and Rod immunodepletion in Xenopus egg extracts

    PMID:20696768

    Open questions at the time
    • Single lab
    • Functional consequence of centromeric SUMOylation downstream of RZZ not defined
  18. 2015 Medium

    Provided a defined biochemical reagent by reconstituting recombinant human RZZ with two copies of each subunit and obtaining crystals, enabling structural analysis.

    Evidence Recombinant insect-cell co-expression, purification and X-ray crystallization

    PMID:25849506

    Open questions at the time
    • Atomic structure not solved in this work
    • Conformational basis of partner switching not addressed
  19. 2019 Medium

    Identified CAL1, the CENP-A chaperone, as a centromeric anchor for RZZ in Drosophila, linking CENP-A loading to checkpoint signaling through ZW10.

    Evidence Co-IP, IF and CAL1 overexpression in Drosophila cultured cells

    PMID:31553715

    Open questions at the time
    • Single lab
    • Whether a human equivalent anchor exists not addressed
  20. 2019 Medium

    Demonstrated that ZW10's checkpoint function operates in mammalian meiosis, with Zw10 needed for Mad2 recruitment and euploid oocyte maturation.

    Evidence siRNA knockdown in mouse oocytes with Mad2 quantification and segregation analysis

    PMID:31250100

    Open questions at the time
    • Single lab
    • Whether RZZ assembly/recruitment in oocytes mirrors mitotic hierarchy not shown
  21. 2024 Medium

    Introduced a post-translational control layer, showing PLK1 phosphorylation of ZW10 at Ser12 dynamically tunes the ZW10–Zwint-1 interaction to balance checkpoint silencing against segregation errors.

    Evidence In vitro PLK1 kinase assay, phospho-mutant/phospho-mimic expression, co-IP and segregation phenotyping

    PMID:38402459

    Open questions at the time
    • Single lab
    • Structural effect of Ser12 phosphorylation on the Zwint-1 interface not resolved
    • Other ZW10 phosphosites/kinases not explored

Open questions

Synthesis pass · forward-looking unresolved questions
  • How a single ZW10 N-terminal hub coordinates partner switching among dynamitin, RINT-1, and Zwint-1 to toggle between mitotic checkpoint/segregation and interphase membrane-trafficking functions, and the atomic structure underlying RZZ assembly and these contacts, remains unresolved.
  • No atomic structure of full RZZ or of ZW10–partner interfaces
  • Cell-cycle signal that reassigns ZW10 between complexes unknown
  • Quantitative partitioning of ZW10 pools across functions undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4 GO:0008092 cytoskeletal protein binding 2
Localization
GO:0005694 chromosome 5 GO:0005783 endoplasmic reticulum 2 GO:0005794 Golgi apparatus 2 GO:0005768 endosome 1 GO:0005829 cytosol 1
Pathway
R-HSA-1640170 Cell Cycle 4 R-HSA-5653656 Vesicle-mediated transport 3 R-HSA-9609507 Protein localization 1
Partners
CAL1DYNAMITINPLK1RINT-1RODSYNTAXIN 18ZWILCHZWINT-1
Complex memberships
RZZ complex (ROD-Zwilch-ZW10)syntaxin 18 SNARE complex (ZW10/RINT-1/p31)

Evidence

Reading pass · 21 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1992 The ZW10 protein (85 kDa) displays cell cycle-dependent localization: excluded from nuclei during interphase, migrates into the nuclear zone at prometaphase, associates with a filamentous structure (possibly kinetochore microtubules) at metaphase, and undergoes rapid redistribution to kinetochore regions at anaphase onset. Loss-of-function causes lagging chromosomes and broken centromeric connections in colchicine-treated cells. Immunofluorescence microscopy, mutant analysis in Drosophila larval brain neuroblasts The Journal of cell biology High 1339459
1994 ZW10 protein localization at kinetochores is dependent on the rough deal and abnormal anaphase resolution gene products, indicating ZW10 functions within a multicomponent pathway. ZW10 accumulates at kinetochores in mitotically arrested cells, and its activity becomes essential at anaphase onset. Genetic epistasis analysis, immunofluorescence in Drosophila mutant backgrounds Journal of cell science High 7914521
1996 ZW10 redistribution from kinetochores to kinetochore microtubules at metaphase requires bipolar spindle attachment. The presence or absence of ZW10 at a kinetochore predicts whether that chromosome moves to the pole, placing ZW10 downstream of or within a tension-sensing mechanism regulating chromosome separation at anaphase onset. Immunofluorescence in Drosophila meiotic cells; analysis of chromosome behavior in multiple mutant backgrounds The Journal of cell biology High 8794856
1997 ZW10 protein is conserved from Drosophila to humans, mice, C. elegans, and plants. Human ZW10 displays the same cell cycle-dependent kinetochore localization as Drosophila ZW10 in HeLa cells. C. elegans ZW10 antisense RNA phenocopies Drosophila zw10 mutations, demonstrating functional conservation of the chromosome segregation role. Cross-species sequence analysis, anti-human ZW10 immunofluorescence in HeLa cells, C. elegans antisense RNA injection The Journal of cell biology High 9298984
1998 ZW10 is required for dynein and dynactin localization to kinetochores in Drosophila. Dynamitin (p50 subunit of dynactin) interacts with ZW10 in a yeast two-hybrid screen. ZW10 and dynein show parallel behavior: both leave kinetochores at metaphase, both bind functional neocentromeres, and both require Rough Deal for kinetochore localization. In zw10 mutants, dynein fails to localize to kinetochores but chromosome congression proceeds normally, suggesting kinetochore dynein is dispensable for microtubule capture but essential for chromosome motion at anaphase. Yeast two-hybrid (ZW10-dynamitin interaction), immunofluorescence in zw10 mutants, analysis of dynein/ZW10 co-behavior The Journal of cell biology High 9700164
1998 Kinetochore localization of Bub3 (spindle assembly checkpoint protein) does not require ZW10 or Rod, demonstrating that kinetochore assembly proceeds through at least two relatively independent pathways. Immunofluorescence in Drosophila zw10 and rod mutant neuroblasts Chromosoma Medium 9914369
2000 ZW10 and Rod together are required for normal poleward chromosome motion rate; zw10-null mutants show greatly attenuated poleward chromosome movement throughout division and highly asynchronous chromosome disjunction at anaphase. Live imaging of chromosome movement in zw10-null Drosophila cells Nature cell biology High 11146661
2001 ROD and ZW10 form a large (~700–900 kDa) evolutionarily conserved macromolecular complex in both Drosophila and human cells. They colocalize throughout mitosis, require each other for recruitment to the mitotic apparatus, and show no additive phenotypic effects in double null mutants, indicating they function in the same pathway. Co-immunoprecipitation from Drosophila and HeLa extracts, size-exclusion chromatography, immunofluorescence, zw10; rod double null mutant analysis Journal of cell science High 11590237
2003 ZW10 and ROD form a trimeric complex with a third subunit, Zwilch. Zwilch localizes to kinetochores identically to ZW10 and ROD. Human Zwilch co-immunoprecipitates with hZW10 and hROD from HeLa extracts. Immunoaffinity chromatography data suggests a weak interaction between the ZW10/ROD/Zwilch (RZZ) complex and the kinesin CENP-meta. Immunoaffinity chromatography, mass spectrometry, co-immunoprecipitation from HeLa extracts, immunofluorescence, Drosophila mutant analysis Molecular biology of the cell High 12686595
2004 During interphase, ZW10 localizes to the endoplasmic reticulum and cytosol, and forms a subcomplex with RINT-1 and p31 within a larger complex containing syntaxin 18 (an ER-localized t-SNARE). ZW10/RINT-1/p31 dissociate from syntaxin 18 upon Mg2+-ATP treatment with NSF and alpha-SNAP (but the subcomplex itself is maintained). ZW10 overexpression, microinjection of antibodies, and ZW10 knockdown each disrupt membrane trafficking between the ER and Golgi. Co-immunoprecipitation, cell fractionation, overexpression, antibody microinjection, siRNA knockdown, Golgi morphology assays The EMBO journal High 15029241
2005 In mitotic human cells, ZW10 resides in a complex with ROD and Zwilch (RZZ complex), while its other partner Zwint-1 is part of a separate structural kinetochore complex with Mis12 and Ndc80/Hec1. Zwint-1 is critical for recruiting ZW10 to unattached kinetochores. Depletion of ZW10 from human cells or Xenopus egg extracts abolishes stable Mad1-Mad2 binding to unattached kinetochores, demonstrating that the RZZ complex bridges structural kinetochore components to the mitotic checkpoint machinery. Co-immunoprecipitation from HeLa mitotic extracts, siRNA depletion in human cells, immunodepletion from Xenopus egg extracts, immunofluorescence The Journal of cell biology High 15824131
2006 Hec1 directly interacts with Zwint-1 in human cells; Hec1 recruits Zwint-1 to kinetochores first (from prophase), after which Zwint-1 recruits ZW10 (from prometaphase). Depletion of Zwint-1 abolishes ZW10 kinetochore localization without affecting Hec1. This establishes a Hec1→Zwint-1→ZW10 sequential recruitment hierarchy at human kinetochores required for spindle checkpoint control. Co-immunoprecipitation (M-phase specific), siRNA depletion of Hec1 and Zwint-1, immunofluorescence Oncogene High 16732327
2006 ZW10 localizes to pericentriolar membranous structures and cosediments with Golgi membranes during interphase. Dominant-negative ZW10, anti-ZW10 antibody injection, and ZW10 RNAi each cause Golgi dispersal, endosome and lysosome dispersal, and a specific decrease in minus-end-directed movements as shown by live imaging. Golgi membrane-associated dynein is markedly decreased after ZW10 RNAi, indicating ZW10 mediates dynein cargo binding to membranes during interphase. Subcellular fractionation (cosedimentation), dominant-negative overexpression, antibody injection, RNAi, live imaging of organelle markers, dynein pulldown from membrane fractions The Journal of cell biology High 16505164
2006 RINT-1 regulates ZW10 localization and its entry into the syntaxin 18 SNARE complex. Overexpression of the N-terminal RINT-1 domain (which binds ZW10) causes ZW10 redistribution, ER-to-Golgi transport block, and Golgi dispersal. RINT-1 knockdown reduces ZW10 association with syntaxin 18. In contrast, ZW10 knockdown does not cause RINT-1 redistribution, establishing RINT-1 as upstream of ZW10 in the ER-Golgi trafficking pathway. Overexpression, siRNA knockdown, co-immunoprecipitation, Golgi morphology assays Molecular biology of the cell High 16571679
2008 In C. elegans, the RZZ complex and the coiled-coil adaptor SPDL-1 both recruit dynein/dynactin to kinetochores and are required for Mad2 targeting and spindle checkpoint activation. RZZ inhibition slows but does not prevent load-bearing kinetochore-microtubule attachments; SPDL-1 inhibition alone (which abolishes dynein targeting without perturbing RZZ localization) prevents load-bearing attachments. Co-inhibition of SPDL-1 and RZZ reduces severity to RZZ-alone levels, indicating RZZ can inhibit load-bearing attachment formation and that dynein (via SPDL-1) normally counteracts this RZZ activity. RNAi epistasis in C. elegans embryos, kinetochore tension assays, immunofluorescence, co-immunoprecipitation Genes & development High 18765790
2008 The N-terminal region of ZW10 (not C-terminal as previously reported) is the major binding site for dynamitin. This N-terminal region can move along microtubules to the centrosomal area in a dynein-dynactin-dependent manner. Competitive binding experiments show dynamitin and RINT-1 bind the same N-terminal region of ZW10 in a mutually exclusive fashion; RINT-1 overexpression interferes with dynein-dynactin-mediated ZW10 movement. The N-terminal region also interacts with Zwint-1, suggesting partner-switching at this domain controls ZW10 localization and dynein-linking function. Yeast two-hybrid, co-immunoprecipitation with truncation mutants, competitive binding assays, live imaging of ZW10 movement Genes to cells Medium 18782227
2010 The Rod/Zw10 complex interacts with the N-terminal domain (first 47 residues) of PIASy (a SUMO E3 ligase) at centromeres. This interaction is required for centromeric localization of PIASy and for PIASy-dependent centromeric SUMOylation (SUMO2/3) during mitosis in Xenopus egg extracts. Depletion of Rod compromises centromeric localization of PIASy and SUMO2/3. Co-immunoprecipitation, truncation mapping, immunofluorescence in Xenopus egg extracts, immunodepletion of Rod The Journal of biological chemistry Medium 20696768
2015 The human Rod-Zwilch-ZW10 (RZZ) complex was recombinantly reconstituted by co-expression in insect cells, purified to homogeneity, and shown to contain two copies of each subunit (~800 kDa predicted). Crystals were obtained in space group P3₁/P3₂, enabling structural studies. Recombinant co-expression in insect cells, biochemical purification, X-ray crystallography (initial crystals) Acta crystallographica Section F Medium 25849506
2019 In Drosophila, CAL1 (CENP-A chaperone) interacts with Zw10 (RZZ complex component) and constitutes the anchor for RZZ complex recruitment to centromeres. This interaction connects CENP-A loading during metaphase to spindle assembly checkpoint signaling through RZZ. Co-immunoprecipitation, immunofluorescence, CAL1 overexpression experiments in Drosophila cultured cells PLoS genetics Medium 31553715
2019 Zw10 localizes to kinetochores during mouse oocyte meiosis and is required for Mad2 recruitment to unattached kinetochores and spindle assembly checkpoint activation. Zw10 knockdown causes precocious polar body extrusion, impaired chromosome alignment, and increased aneuploidy. siRNA knockdown in mouse oocytes, immunofluorescence, quantification of Mad2 kinetochore signals, chromosome segregation analysis Histochemistry and cell biology Medium 31250100
2024 PLK1 phosphorylates ZW10 at Ser12, and this phosphorylation is required for dynamic ZW10-Zwint1 interactions. Inhibition of ZW10 phosphorylation causes misaligned chromosomes, while persistent phospho-mimicking ZW10 causes premature anaphase with entangled sister chromatids, demonstrating that PLK1-mediated ZW10 phosphorylation fine-tunes spindle checkpoint silencing and accurate chromosome segregation. In vitro kinase assay (PLK1-ZW10), phospho-mutant and phospho-mimicking ZW10 expression, co-immunoprecipitation, chromosome segregation phenotype analysis Journal of molecular cell biology Medium 38402459

Source papers

Stage 0 corpus · 35 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1998 ZW10 helps recruit dynactin and dynein to the kinetochore. The Journal of cell biology 221 9700164
2005 ZW10 links mitotic checkpoint signaling to the structural kinetochore. The Journal of cell biology 214 15824131
2005 Rod-Zw10-Zwilch: a key player in the spindle checkpoint. Trends in cell biology 176 15922598
2004 Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi. The EMBO journal 160 15029241
2008 A new mechanism controlling kinetochore-microtubule interactions revealed by comparison of two dynein-targeting components: SPDL-1 and the Rod/Zwilch/Zw10 complex. Genes & development 144 18765790
1992 The Drosophila l(1)zw10 gene product, required for accurate mitotic chromosome segregation, is redistributed at anaphase onset. The Journal of cell biology 113 1339459
2000 The rate of poleward chromosome motion is attenuated in Drosophila zw10 and rod mutants. Nature cell biology 111 11146661
1997 Conservation of the centromere/kinetochore protein ZW10. The Journal of cell biology 91 9298984
2003 Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions. Molecular biology of the cell 86 12686595
1996 Bipolar spindle attachments affect redistributions of ZW10, a Drosophila centromere/kinetochore component required for accurate chromosome segregation. The Journal of cell biology 84 8794856
1998 Localization of the Drosophila checkpoint control protein Bub3 to the kinetochore requires Bub1 but not Zw10 or Rod. Chromosoma 80 9914369
2007 Rab6 regulates both ZW10/RINT-1 and conserved oligomeric Golgi complex-dependent Golgi trafficking and homeostasis. Molecular biology of the cell 79 17699596
2001 The ZW10 and Rough Deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore. Journal of cell science 73 11590237
2006 RINT-1 regulates the localization and entry of ZW10 to the syntaxin 18 complex. Molecular biology of the cell 68 16571679
1994 Determinants of Drosophila zw10 protein localization and function. Journal of cell science 64 7914521
2006 Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control. Oncogene 55 16732327
2006 Role of the kinetochore/cell cycle checkpoint protein ZW10 in interphase cytoplasmic dynein function. The Journal of cell biology 50 16505164
2010 Dsl1p/Zw10: common mechanisms behind tethering vesicles and microtubules. Trends in cell biology 46 20226673
2006 ZW10 function in mitotic checkpoint control, dynein targeting and membrane trafficking: is dynein the unifying theme? Cell cycle (Georgetown, Tex.) 38 17102640
2014 Distinct sets of Rab6 effectors contribute to ZW10--and COG-dependent Golgi homeostasis. Traffic (Copenhagen, Denmark) 23 24575842
2021 The antagonistic mechanism of Bacillus velezensis ZW10 against rice blast disease: Evaluation of ZW10 as a potential biopesticide. PloS one 21 34449822
2010 Rod/Zw10 complex is required for PIASy-dependent centromeric SUMOylation. The Journal of biological chemistry 20 20696768
2020 Comprehensive analysis of 5-hydroxymethylcytosine in zw10 kinetochore protein as a promising biomarker for screening and diagnosis of early colorectal cancer. Clinical and translational medicine 17 32628818
2004 Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes. Gene 17 15094189
2008 N-terminal region of ZW10 serves not only as a determinant for localization but also as a link with dynein function. Genes to cells : devoted to molecular & cellular mechanisms 13 18782227
2019 The checkpoint protein Zw10 connects CAL1-dependent CENP-A centromeric loading and mitosis duration in Drosophila cells. PLoS genetics 11 31553715
2024 PLK1 phosphorylation of ZW10 guides accurate chromosome segregation in mitosis. Journal of molecular cell biology 10 38402459
2022 Zw10 negatively regulates the MyD88-mediated NF-κB signaling through autophagy in teleost fish. Developmental and comparative immunology 7 35339534
2019 Zw10 is a spindle assembly checkpoint protein that regulates meiotic maturation in mouse oocytes. Histochemistry and cell biology 5 31250100
2015 Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod-Zwilch-ZW10 (RZZ) complex. Acta crystallographica. Section F, Structural biology communications 5 25849506
2001 Gene structure, chromosomal localization and immunolocalization of chicken centromere proteins CENP-C and ZW10. Gene 5 11179694
2024 ZW10: an emerging orchestrator of organelle dynamics during the cell division cycle. Journal of molecular cell biology 4 38830800
2024 A multidimensional analysis of ZW10 interacting kinetochore protein in human tumors. American journal of cancer research 3 38323280
2019 Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues. Journal of visualized experiments : JoVE 2 31107455
2017 Rs2459976 in ZW10 gene associated with congenital heart diseases in Chinese Han population. Oncotarget 1 29423089

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