| 2016 |
ZDHHC7 is the major palmitoyl acyltransferase that S-palmitoylates Scribble (SCRIB) at conserved cysteine residues, which is required for SCRIB membrane targeting to cell-cell junctions, maintenance of epithelial cell polarity, and tumor suppression. Knockout of ZDHHC7 causes SCRIB mislocalization and YAP activation, and disrupts SCRIB's suppressive activities in HRas(V12)-induced cell invasion. |
Chemical reporter palmitoylation assay, ZDHHC7 knockout, palmitoylation-deficient SCRIB mutants, cell polarity assays, invasion assays |
Nature chemical biology |
High |
27380321
|
| 2024 |
ZDHHC7 palmitoylates NLRP3 at Cys126, promoting NLRP3 localization on the trans-Golgi network (TGN) at rest and on dispersed TGN upon activation, which is required for recruitment and oligomerization of the adaptor ASC and full inflammasome activation in macrophages and in vivo. |
ZDHHC7 knockout, pharmacological inhibition, Cys126 site mutation, palmitoylation assay, subcellular localization imaging, ASC oligomerization assay, in vivo inflammasome activation |
Cell reports |
High |
38583156
|
| 2024 |
ZDHHC7 S-palmitoylates ATG16L1 at Cys153; this palmitoylation enhances formation of the ATG16L1-WIPI2B and ATG16L1-RAB33B complexes on the phagophore, facilitating LC3 lipidation and autophagosome formation. |
Acyl-biotin exchange assay, ATG16L1-KO HeLa cell rescue with wild-type vs. C153S mutant, ZDHHC7 overexpression/deletion, LC3-II immunoblot, co-immunoprecipitation, transmission electron microscopy |
Autophagy |
High |
39087410
|
| 2023 |
ZDHHC7 inhibits androgen receptor (AR) gene transcription, thereby reducing AR protein levels and abolishing AR signaling in prostate cancer cells; ZDHHC7 depletion increases oncogenic properties while ZDHHC7 restoration suppresses proliferation and invasion in vitro and tumor growth in vivo. |
RNA-seq, ZDHHC7 knockdown/overexpression, AR transcription and protein level assays, xenograft tumor model |
Oncogene |
Medium |
37198397
|
| 2022 |
The transcription factor KLF10 transcriptionally activates ZDHHC7 expression, which then palmitoylates CD36, promoting its plasma membrane localization and driving hepatic lipid accumulation and inflammation in NASH pathogenesis. |
Transcriptomic analysis, gain/loss-of-function in hepatocyte-specific mouse models, CD36 palmitoylation assay, membrane fractionation, diet-induced NASH model |
EMBO reports |
Medium |
35492028
|
| 2019 |
ZDHHC7 deficiency in mice impairs excitatory transmission, synaptic plasticity at hippocampal Schaffer collateral CA1 synapses, and hippocampal structural connectivity in both sexes; it also causes sex-specific effects on medial prefrontal cortical synaptic transmission and anxiety-related behavior (females only), consistent with a role in palmitoylation of sex steroid receptors linking them to membrane microdomains. |
Constitutive Zdhhc7 knockout mouse, behavioral tests, electrophysiology, protein analyses, diffusion tensor-based fiber tractography neuroimaging |
Brain structure & function |
Medium |
31183559
|
| 2024 |
zDHHC7 expression and protein S-palmitoylation are elevated in hippocampi of 3×Tg-AD mice and post-mortem AD patients; hippocampal silencing of zDHHC7 prevents cognitive deficits in 3×Tg-AD mice; a FoxO1-mediated epigenetic mechanism drives zDHHC7 expression in response to brain insulin resistance; and S-palmitoylation of BACE1 (Beta-Secretase 1) by zDHHC7 is associated with Aβ1-42 load. |
zDHHC7 hippocampal silencing (knockdown) in 3×Tg-AD mice, cognitive behavior tests, 2-bromopalmitate pharmacological inhibition, palmitoylation assays, human post-mortem tissue analysis |
Proceedings of the National Academy of Sciences of the United States of America |
Medium |
39589870
|
| 2025 |
ZDHHC7 palmitoylates Sprouty 4 (SPRY4), and this modification is reversed by palmitoyl-protein thioesterase 1 (PPT1); the dynamic palmitoylation-depalmitoylation cycle of SPRY4 modulates MAPK signaling and affects tumor cell proliferation, migration, apoptosis, and cisplatin resistance in osteosarcoma cells. |
In vitro and in vivo experiments, single-cell analysis, palmitoylation assays, MAPK signaling readouts, drug resistance assays |
Research (Washington, D.C.) |
Low |
40416361
|
| 2026 |
ZDHHC7 and ZDHHC3 (both Golgi-localized S-acyltransferases) mediate triple S-acylation of the small GTPase ARL15 at Cys17, Cys22, and Cys23; loss of S-acylation disrupts ARL15 membrane association, redistributing it from membranes to the cytosol. |
Acyl-PEGyl exchange gel-shift assay, siRNA knockdown, CRISPR/Cas9 gene disruption, confocal imaging, subcellular fractionation, cysteine-to-serine mutagenesis |
The Journal of biological chemistry |
High |
41999893
|
| 2026 |
ZDHHC7 S-palmitoylates the PRRSV nonstructural protein Nsp10 in an acyltransferase-dependent manner, weakening Nsp10 binding to viral RNA and restricting PRRSV replication; PRRSV counteracts this by having Nsp4 cleave ZDHHC7 at E226 (abolishing palmitoylation of Nsp10, STING, and MAVS), and by Nsp2/GP5 inducing APT1 (depalmitoylase) via NFATC3 to reverse Nsp10 palmitoylation. |
Palmitoylation assays, ZDHHC7 overexpression/mutation (E226A cleavage-resistant), viral replication assays, RNA-protein binding assays, transcription factor analysis |
Research in veterinary science |
Medium |
41671757
|
| 2021 |
ZDHHC7 deficiency impairs synaptic plasticity in mice of both sexes; after acute stress, male Zdhhc7-KO mice show reduced mean fiber lengths of the medioventral hippocampus, while acute stress improves synaptic plasticity in female (but not male) mice; estrogen receptor α and β mRNA levels are reduced by acute stress in male mice regardless of genotype, consistent with ZDHHC7 modulating stress responses partly via estrogen receptor-mediated pathways. |
Zdhhc7 knockout mouse, electrophysiology, diffusion tensor imaging, mRNA expression analysis (qPCR), behavioral tests |
Brain structure & function |
Low |
33880616
|