Affinage

WNT10B

Protein Wnt-10b · UniProt O00744

Length
389 aa
Mass
43.0 kDa
Annotated
2026-06-11
100 papers in source corpus 37 papers cited in narrative 37 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

WNT10B is a secreted canonical Wnt ligand that controls mesenchymal progenitor cell fate, directing precursors toward osteoblastogenesis (via induction of Runx2, Dlx5, and osterix) while suppressing adipogenesis (via repression of C/EBPα and PPARγ), with reciprocal gain- and loss-of-function in mice establishing its endogenous role in bone formation and fat restraint (PMID:15728361, PMID:15190075, PMID:20499361). These fate-determining effects on mesenchymal stem cells require β-catenin, since β-catenin knockdown abolishes both the pro-osteogenic and anti-adipogenic activities of WNT10B (PMID:21872687), and a naturally occurring obesity-associated C256Y variant loses canonical signaling capacity (PMID:16477437). In the skeletal anabolic axis, WNT10B is produced by bone marrow CD8+ T cells in response to intermittent parathyroid hormone and to gut microbiota-derived butyrate acting through Treg cells, the latter driving an NFAT1-SMAD3 transcription complex that induces Wnt10b; T-cell-derived WNT10B then activates canonical Wnt signaling in preosteoblasts and mediates the sclerostin-independent component of iPTH bone anabolism (PMID:19723499, PMID:30446387, PMID:24357520). Beyond β-catenin, WNT10B signals through β-catenin-independent routes: a Wnt/STOP (LRP6-DVL-dependent) pathway that suppresses GSK3β to maintain mitotic microtubule dynamics and chromosome segregation fidelity (PMID:33257473), and nuclear RAC1 transport that drives Cyclin D1-dependent proliferation (PMID:26370090). WNT10B can be trafficked via fibroblast-derived exosomes for paracrine and autocrine action, including mTOR activation that supports axonal regeneration after optic nerve injury (PMID:28394344, PMID:28683327). In cancer, WNT10B activates β-catenin to upregulate HMGA2 and sustain a β-CATENIN/HMGA2/EZH2 autoregulatory loop driving proliferation and chemoresistance (PMID:23307470, PMID:30563890). Its expression is tightly controlled epigenetically by NSD1-mediated H3K36me2, BMI1-mediated H2AK119ub, and MAT2A-EZH2-mediated H3K27me3, and post-transcriptionally by miR-148a acting downstream of XBP1s (PMID:31727171, PMID:35541907, PMID:29133280, PMID:27055562, PMID:23603388).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1997 High

    Established WNT10B as a developmentally and oncogenically active Wnt ligand in vivo, before its mesenchymal fate role was known.

    Evidence MMTV-Wnt10b transgenic mice with mammary phenotyping and an FGF-3 genetic cross

    PMID:9393971

    Open questions at the time
    • Did not define the signaling pathway (β-catenin vs other)
    • Did not identify direct transcriptional targets
  2. 2003 High

    Showed WNT10B has a conserved developmental patterning function redundant with Wnt1 at the midbrain-hindbrain boundary.

    Evidence Zebrafish wnt1-wnt10b deficiency and morpholino knockdown with in situ hybridization and epistasis to Pax2.1/Fgf8

    PMID:12591239

    Open questions at the time
    • Redundancy with Wnt1 obscures Wnt10b-specific requirement
    • No biochemical pathway analysis
  3. 2005 High

    Defined the core function: WNT10B switches mesenchymal precursor fate toward bone and away from fat via defined transcription factor targets, and is required endogenously for trabecular bone.

    Evidence FABP4-Wnt10b transgenic and Wnt10b knockout mice with histomorphometry and adipose/metabolic phenotyping

    PMID:15190075 PMID:15673614 PMID:15728361

    Open questions at the time
    • Did not pin the effect on β-catenin causally
    • Receptor context for WNT10B not defined
  4. 2006 Medium

    Linked WNT10B loss-of-canonical-signaling directly to a human obesity-associated variant, validating the anti-adipogenic mechanism in humans.

    Evidence Functional assay of the C256Y missense variant for canonical signaling and adipogenesis block

    PMID:16477437

    Open questions at the time
    • Single-variant functional test
    • No structural basis for loss of activity
  5. 2009 High

    Identified an immune-skeletal axis in which iPTH stimulates CD8+ T-cell WNT10B to drive osteoblast commitment, redefining the cellular source of bone-anabolic Wnt.

    Evidence iPTH treatment in T-cell-null and T-cell-specific Wnt10b conditional knockout mice with histomorphometry

    PMID:19723499

    Open questions at the time
    • Did not resolve receptor on preosteoblasts
    • Mechanism of T-cell Wnt10b induction not yet known
  6. 2011 High

    Established β-catenin as strictly required for WNT10B control of MSC fate via genetic epistasis.

    Evidence β-catenin knockdown in ST2 and 3T3-L1 cells with osteogenic/adipogenic readouts

    PMID:21872687

    Open questions at the time
    • Did not exclude parallel β-catenin-independent contributions in other cell types
  7. 2013 High

    Connected WNT10B to oncogenic transcription by identifying HMGA2 as a direct β-catenin target mediating TNBC proliferation.

    Evidence ChIP, HMGA2 siRNA epistasis, luciferase reporters and a transgenic tumor model

    PMID:23307470

    Open questions at the time
    • HMGA2 downstream effectors not fully defined
    • Generalizability beyond TNBC unclear
  8. 2013 Medium

    Expanded the regulatory inputs on WNT10B, showing hypoxia (HIF-2α), TGF-β1/Smad in osteoclasts, SREBP-1c reciprocity, XBP1 repression, and CD40L-dependent T-cell production each control its expression or downstream metabolic output.

    Evidence ChIP, conditioned-media assays, signaling blockades and knockout/knockdown across multiple cell systems

    PMID:20041157 PMID:23603388 PMID:23861379 PMID:23900840 PMID:23954891

    Open questions at the time
    • Each axis from a single lab
    • Cross-talk and hierarchy among these regulators not integrated
  9. 2016 Medium

    Validated WNT10B as a Mendelian disease gene, linking loss-of-canonical-signaling mutations to oligodontia.

    Evidence Patient mutation sequencing with TOPFlash reporter and dental pulp stem cell differentiation assays

    PMID:27321946

    Open questions at the time
    • Genotype-phenotype correlation across mutation types not resolved
    • Developmental cell target in tooth formation undefined
  10. 2017 Medium

    Showed WNT10B is delivered via exosomes for paracrine and autocrine signaling, enabling EMT-driven cancer progression and mTOR-dependent axonal regeneration.

    Evidence Exosome isolation, lipid raft fractionation, co-culture and Wnt10b knockout in an optic nerve injury model

    PMID:28394344 PMID:28683327

    Open questions at the time
    • Exosomal loading/sorting mechanism unknown
    • Receptor engagement on recipient cells not defined
  11. 2018 High

    Defined the upstream transcriptional logic of T-cell WNT10B (butyrate→Treg→NFAT1-SMAD3) and a β-CATENIN/HMGA2/EZH2 autoregulatory loop sustaining chemoresistant cancer.

    Evidence Germ-free/Treg-depleted mice, Wnt10b-/- CD8+ T-cell reconstitution, ChIP, Co-IP and PDX models

    PMID:30446387 PMID:30563890

    Open questions at the time
    • K49 acetylation regulation single-lab
    • Translation of microbiota axis to humans not established here
  12. 2020 Medium

    Revealed a β-catenin-independent role: WNT10B uses Wnt/STOP through LRP6-DVL-GSK3β to safeguard mitotic spindle dynamics and prevent aneuploidy.

    Evidence Wnt10b loss-of-function in human somatic cells with live-cell imaging of mitosis and microtubule dynamics

    PMID:33257473

    Open questions at the time
    • GSK3β substrates mediating microtubule effect not defined
    • Single lab
  13. 2022 Medium

    Consolidated epigenetic control of the Wnt10b locus, adding BMI1-H2AK119ub repression in stem cell maintenance alongside NSD1 and MAT2A-EZH2 mechanisms.

    Evidence BMI1 knockout mice with histone-mark ChIP and β-catenin-inhibition rescue in spermatogonial stem cells

    PMID:29133280 PMID:31727171 PMID:35541907

    Open questions at the time
    • How distinct chromatin writers are coordinated at the locus is unknown
    • Tissue specificity of each mechanism unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • The receptor/co-receptor complex through which WNT10B engages target cells, and how a single ligand partitions between canonical β-catenin, Wnt/STOP, and RAC1 outputs in a given cell, remain undefined.
  • No defined Frizzled receptor for WNT10B in the corpus
  • Mechanism selecting among parallel pathways not established
  • Structural basis of ligand-receptor engagement absent

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0048018 receptor ligand activity 4 GO:0060089 molecular transducer activity 3
Localization
GO:0005576 extracellular region 4 GO:0031410 cytoplasmic vesicle 2 GO:0005929 cilium 1
Pathway
R-HSA-1266738 Developmental Biology 4 R-HSA-162582 Signal Transduction 4 R-HSA-1643685 Disease 4 R-HSA-74160 Gene expression (Transcription) 4
Partners
CTNNB1GSK3BLRP6RAC1

Evidence

Reading pass · 37 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2005 Wnt10b shifts mesenchymal precursor cell fate toward osteoblastogenesis and away from adipogenesis by inducing osteoblastogenic transcription factors Runx2, Dlx5, and osterix while suppressing adipogenic transcription factors C/EBPα and PPARγ; Wnt10b-/- mice have decreased trabecular bone confirming its endogenous role in bone formation. Transgenic mouse overexpression (FABP4-Wnt10b), Wnt10b knockout mice, pharmacological and genetic epistasis approaches, histomorphometry Proceedings of the National Academy of Sciences of the United States of America High 15728361
2004 Wnt10b inhibits development of both white and brown adipose tissue in vivo; transgenic FABP4-Wnt10b mice show ~50% reduction in total body fat, resistance to diet-induced obesity, improved glucose tolerance and insulin sensitivity, and inability to maintain core body temperature (loss of brown adipose tissue function). Transgenic mouse model (FABP4-Wnt10b), histology, metabolic phenotyping, cold-exposure challenge The Journal of biological chemistry High 15190075
2011 Wnt10b inhibits adipogenesis and stimulates osteoblastogenesis through a β-catenin-dependent mechanism; knockdown of β-catenin completely prevents both effects of Wnt10b on mesenchymal stem cell fate in bipotential ST2 cells and 3T3-L1 preadipocytes. Gain- and loss-of-function in ST2 and 3T3-L1 cells, β-catenin knockdown, osteoblastogenesis and adipogenesis assays Bone High 21872687
2009 Intermittent parathyroid hormone (iPTH) increases Wnt10b production by bone marrow CD8+ T cells, which then activate canonical Wnt signaling in preosteoblasts to stimulate osteoblastic commitment, proliferation, differentiation, and lifespan; T-cell-null mice and mice lacking T-cell-produced Wnt10b show no anabolic response to iPTH. In vivo iPTH treatment, T-cell-null mice, conditional Wnt10b knockout in T cells, histomorphometry, Wnt signaling assays in preosteoblasts Cell metabolism High 19723499
2018 Gut microbiota-derived butyrate induces expansion of intestinal and bone marrow regulatory T (Treg) cells; Treg cells promote assembly of a NFAT1-SMAD3 transcription complex in CD8+ T cells, driving expression of Wnt10b, which mediates bone anabolic effects; reconstitution with Wnt10b-/- CD8+ T cells prevents butyrate-induced bone formation. Probiotic/butyrate supplementation in mice, germ-free mice, Treg depletion, TCRβ-/- mice reconstituted with Wnt10b-/- CD8+ T cells, ChIP for NFAT1-SMAD3 complex, microCT Immunity High 30446387
2013 WNT10B activates canonical β-catenin signaling in triple-negative breast cancer cells, leading to transcriptional upregulation of HMGA2 (demonstrated by ChIP); HMGA2 is necessary and sufficient for WNT10B-driven proliferation of TNBC cells; siRNA to HMGA2 decreases proliferation. ChIP analysis, siRNA knockdown, luciferase reporter assays, transgenic mouse tumor model, Western blotting EMBO molecular medicine High 23307470
2018 In chemoresistant TNBC, WNT10B activates a β-CATENIN/HMGA2/EZH2 autoregulatory loop; HMGA2 and EZH2 displace Groucho/TLE1 from TCF-4 and serve as gatekeepers for K49 acetylation on β-CATENIN essential for transcription; HMGA2-EZH2 interacts with the PRC2 complex. Co-immunoprecipitation, MMTV-Wnt10b transgenic mice, Hmga2 haploinsufficiency, PDX model, Western blot, molecular epistasis Cancer research Medium 30563890
2013 TGF-β1 stimulates Wnt10b production in osteoclasts through Smad2/3 activation (not AKT or MAPK); TGF-β1-induced osteoclast-derived Wnt10b promotes osteoblast mineralization; blocking Wnt10b with DKK1 suppresses this mineralization-promoting activity. In vitro osteoclast culture, TGF-β1 treatment, Smad2/3 signaling blockade, DKK1 inhibition, conditioned media osteoblast mineralization assay Endocrinology Medium 23861379
2010 Wnt10b deficiency in null mice results in a progressive, age-dependent reduction in mesenchymal progenitor cells (assessed by colony-forming unit assays) and trabecular bone loss, with reduced expression of osteoblast differentiation markers in bone marrow stromal cells. Wnt10b-/- mice, microCT, histomorphometry, colony-forming unit assays, osteogenic gene expression analysis Journal of bone and mineral research High 20499361
2005 Wnt10b deficiency in myoblasts increases adipogenic potential, contributing to excessive lipid accumulation in regenerating myofibers; overexpression of Wnt10b in aged myoblasts inhibits adipogenic gene expression and sustains myogenic differentiation; GSK-3 inhibition mimics Wnt10b overexpression effects. Wnt10b null myoblasts, Wnt10b overexpression, GSK-3 inhibition, adipogenic/myogenic differentiation assays, in vivo muscle regeneration analysis Molecular biology of the cell High 15673614
1997 Overexpression of Wnt-10b in mammary glands of transgenic mice (MMTV-Wnt10b) causes hypermorphic ductal development, precocious alveolar formation in virgins, male gynecomastia bypassing androgen repression, and high susceptibility to mammary adenocarcinoma; co-expression with FGF-3/int-2 causes potent interaction and disorganized mammary epithelium. MMTV-Wnt10b transgenic mouse model, histology, tumor incidence analysis, MMTV-Wnt10b × MMTV-FGF3 cross Oncogene High 9393971
2017 Wnt10b secreted from fibroblasts is transported via exosomes to breast cancer epithelial cells; paracrine Wnt10b from p85α-deficient fibroblasts promotes cancer progression through epithelial-to-mesenchymal transition via the canonical Wnt pathway. Conditioned media experiments, exosome isolation/characterization, co-culture, EMT marker analysis, Western blot Oncogene Medium 28394344
2017 Fibroblast-derived exosomes (FD exosomes) mobilize Wnt10b toward lipid rafts, activating mTOR signaling via GSK3β and TSC2; autocrine Wnt10b-mTOR pathway is required for FD exosome-promoted axonal regeneration after optic nerve injury, as Wnt10b-deleted animals show strongly reduced regeneration. Wnt10b knockout mice, optic nerve injury model, lipid raft fractionation, mTOR signaling pathway analysis, in vitro neurite growth assays Cell reports Medium 28683327
2012 miR-148a directly targets WNT10B mRNA via its 3'UTR (demonstrated by luciferase reporter assay); silencing of miR-148a in cancer-associated fibroblasts elevates WNT10B protein levels and promotes migration of endometrial cancer cell lines; re-introduction of miR-148a reduces WNT10B and impairs cancer cell migration. 3'UTR luciferase reporter assay, miR-148a lentiviral overexpression, conditioned media migration assays, Western blot Oncogene High 22890324
2003 In zebrafish, wnt10b and wnt1 provide partially redundant functions at the midbrain-hindbrain boundary (MHB); double deletion of both loci causes loss of pax2.1, en2, and her5 expression in the ventral MHB; wnt10b/wnt1 are required to maintain threshold levels of Pax2.1 and Fgf8 at the MHB. Zebrafish wnt1-wnt10b deficiency allele generation, morpholino knockdown, in situ hybridization, genetic epistasis with Pax2.1 and Fgf8 mutants Developmental biology High 12591239
2020 Wnt10b is required for normal mitotic microtubule dynamics and chromosome segregation in human somatic cells; Wnt10b acts through a Wnt/STOP (LRP6-DVL-dependent, β-catenin-independent) pathway to suppress GSK3β activity; loss of Wnt10b causes increased microtubule growth rates in mitotic spindles, whole chromosome missegregation, and aneuploidy. Wnt10b knockdown/knockout in human somatic cells, live-cell imaging of mitosis, microtubule dynamics measurements, Wnt/STOP pathway analysis, chromosome segregation assays Life science alliance Medium 33257473
2015 WNT10B causes nuclear transport and binding of both RAC1 and β-catenin in human corneal endothelial cells, activating Cyclin D1 expression and proliferation through simultaneously β-catenin-dependent and β-catenin-independent (RAC1) pathways. Nuclear fractionation, co-immunoprecipitation, reporter assays, proliferation assays in human corneal endothelial cells The Journal of biological chemistry Medium 26370090
2014 The sclerostin-independent bone anabolic activity of iPTH is mediated exclusively by T-cell-produced Wnt10b; in T-cell-null mice and mice lacking T-cell Wnt10b expression, combined iPTH + anti-sclerostin antibody treatment is equivalent to anti-sclerostin antibody alone in increasing osteoblast pool and bone volume. T-cell-null mice, T-cell-specific Wnt10b knockout mice, anti-sclerostin antibody treatment, iPTH treatment, histomorphometry, microCT Journal of bone and mineral research High 24357520
2019 NSD1 histone methyltransferase promotes Wnt10b expression by mediating H3K36me2 methylation at the Wnt10b locus; NSD1 knockout increases H3K27me3 and reduces H3K36me2, suppressing Wnt10b expression and inactivating the Wnt/β-catenin pathway in hepatocellular carcinoma cells. CRISPR/Cas9 NSD1 knockout, ChIP for histone marks at Wnt10b locus, Western blot, reporter assays, xenograft model Journal of experimental & clinical cancer research Medium 31727171
2013 HIF-2α (but not HIF-1α) directly binds the Wnt10b enhancer region under hypoxic conditions to drive Wnt10b expression in adipogenic cells; hypoxia-conditioned medium containing Wnt10b activates canonical Wnt signaling (LRP6 phosphorylation, β-catenin-dependent transcription) and inhibits adipogenesis in normoxic cells. ChIP for HIF-2α at Wnt10b enhancer, HIF-2α-deficient cells, hypoxia-conditioned medium, Wnt reporter assays, LRP6 phosphorylation assay The Journal of biological chemistry Medium 23900840
2016 XBP1s directly induces transcription of miR-148a by binding to a response element in the miR-148a promoter (demonstrated by point mutation analysis and ChIP); miR-148a binds the 3'UTR of Wnt10b mRNA to suppress Wnt10b expression and β-catenin activity during adipogenesis, constituting a post-transcriptional silencing axis XBP1s → miR-148a → Wnt10b. ChIP for XBP1s at miR-148a promoter, point mutation of XBP1 response element, 3'UTR reporter assay, miR-148a mimic/knockdown in 3T3-L1 cells Experimental & molecular medicine Medium 27055562
2013 XBP1 transcription factor directly binds the Wnt10b promoter and suppresses Wnt10b expression, reducing β-catenin signaling and promoting adipogenic differentiation of 3T3-L1 preadipocytes; XBP1 and Wnt10b display reciprocal expression patterns during adipogenesis. XBP1 ChIP on Wnt10b promoter, reporter assays, siRNA knockdown, Western blot for β-catenin, 3T3-L1 adipogenesis assay FEBS letters Medium 23603388
2017 MAT2A interacts with Ezh2 and MafK and is recruited to the Wnt10b promoter to repress Wnt10b expression by promoting H3K27 methylation; MAT2A-mediated suppression of Wnt10b inhibits Wnt/β-catenin signaling and promotes adipogenesis. Co-immunoprecipitation of MAT2A-Ezh2-MafK, ChIP for H3K27me3 and MAT2A at Wnt10b promoter, MAT2A overexpression/knockdown, adipogenesis assays Biochimica et biophysica acta. Molecular and cell biology of lipids Medium 29133280
2019 Wnt10b is localized at the base of primary cilia in osteoblasts; sinusoidal electromagnetic fields (SEMFs) cause Wnt10b to disappear from/be released from primary cilia, activating Wnt/β-catenin signaling in osteoblasts; abrogation of primary cilia by IFT88 siRNA blocks SEMF-mediated Wnt10b/β-catenin activation and osteogenic differentiation. IFT88 siRNA knockdown, immunofluorescence localization of Wnt10b at primary cilia, Wnt10b siRNA, osteoblast differentiation assays in rat calvarial osteoblasts, in vivo microCT in rats Journal of bone and mineral research Medium 30779853
2015 After myocardial infarction, Wnt10b is expressed in cardiomyocytes and localizes to intercalated discs; Wnt10b is transiently induced in peri-infarct cardiomyocytes; Wnt10b gain-of-function promotes cardiac repair by stimulating VEGFR2 expression in endothelial cells and angiopoietin-1 in vascular smooth muscle cells through NF-κB activation, coordinating neovascularization and reducing fibrosis. Histological localization in mouse and human hearts, coronary artery ligation/cryoinjury models, cardiomyocyte-specific Wnt10b gain-of-function mouse line, VEGFR2/Ang-1 expression analysis, NF-κB reporter assays Circulation research Medium 26338900
2009 In skeletal muscle cells, Wnt10b and SREBP-1c show reciprocal expression; knockdown of SREBP-1 induces Wnt10b expression and activates Wnt/β-catenin pathway; silencing Wnt10b induces SREBP-1c expression; Wnt/β-catenin activation increases insulin sensitivity by decreasing intramyocellular lipid deposition through SREBP-1c downregulation and increasing glucose transport via differential Akt and AMPK activation. siRNA knockdown of SREBP-1 and Wnt10b, Wnt10b overexpression, GSK-3β inhibition, glucose transport assays, Akt/AMPK pathway analysis in myotubes PloS one Medium 20041157
2022 BMI1 epigenetically represses Wnt10b transcription in spermatogonial stem cells (SSCs) by increasing H2AK119ub and decreasing H3K4me3 at the Wnt10b locus; BMI1 inhibition leads to Wnt10b upregulation, nuclear translocation of β-catenin, and impaired SSC maintenance; suppression of Wnt/β-catenin signaling restores SSC maintenance in BMI1-deficient SSCs. BMI1 knockout mouse model, chromatin modification analysis (H2AK119ub, H3K4me3), β-catenin localization, Wnt/β-catenin inhibition, SSC functional assays in vitro and in vivo International journal of biological sciences Medium 35541907
2007 WNT10B activates β-catenin/Tcf signaling but also has β-catenin/Tcf-independent growth suppression activity; fibroblast growth factor (FGF) synergizes with WNT10B to switch it from growth suppressor to growth promoter; WNT10B is silenced by promoter DNA methylation in 46% of primary hepatocellular carcinomas. WNT10B overexpression in cancer cell lines, dominant-negative hTcf-4, mutant β-catenin transduction, anchorage-independent growth assay, 5-aza-2'deoxycytidine treatment, methylation analysis, FGF co-treatment Molecular biology of the cell Medium 17761539
2007 WNT10B treatment of osteosarcoma cells (U2OS) activates Wnt, NF-κB, and Notch pathways; Wnt10b (but not Wnt3a) upregulates Notch-1, Jagged-1, and activates Notch-responsive genes Hes-1 and Hey-1, and stimulates NF-κB reporter activity; IL-1α and TNF-α are upregulated at transcript and protein levels. Wnt10b-expressing U2OS cell line, microarray analysis, NF-κB reporter assay, Hey-1 reporter assay, qRT-PCR, Western blot, comparison with Wnt3a Journal of cellular biochemistry Medium 21321991
2006 WNT10B C256Y naturally-occurring missense variant abrogates the ability of WNT10B to activate canonical WNT signaling and block adipogenesis in functional assays, establishing loss of canonical signaling as the mechanism underlying this obesity-associated mutation. Functional assay of WNT10B C256Y mutant for canonical WNT signaling activation and adipogenesis inhibition, comparison to wild-type Diabetologia Medium 16477437
2016 WNT10B mutations identified in oligodontia patients (p.Arg211Gln, p.Pro190Arg, p.Trp262*, p.Phe284Cys) cannot normally enhance canonical Wnt signaling in HEPG2 cells (TOPFlash reporter) and cannot efficiently induce endothelial differentiation of dental pulp stem cells. TOPFlash luciferase reporter assay, dental pulp stem cell endothelial differentiation assay, Sanger sequencing American journal of human genetics Medium 27321946
2012 Wnt10b-induced hair follicle regeneration in vivo requires canonical Wnt signaling: Wnt10b causes nuclear translocation of β-catenin, and knockdown of β-catenin with siRNA abrogates Wnt10b-induced hair follicle telogen-to-anagen transition; siRNA knockdown of Wnt10b blocks anagen onset. Intradermal injection of adenovirus-Wnt10b in mice, β-catenin siRNA co-treatment, Wnt10b siRNA, immunofluorescence for nuclear β-catenin, hair follicle cycling assessment The Journal of investigative dermatology Medium 22832493
2013 Ovariectomy expands short-term hematopoietic stem cells (ST-HSPCs) through CD40L expressed on T cells; CD40L is required for ovariectomy to stimulate T-cell Wnt10b production; Wnt10b activates Wnt signaling in HSPCs and stromal cells; ovariectomy fails to expand ST-HSPCs in CD40L-null mice and in mice lacking T-cell Wnt10b. Ovariectomy in CD40L-null mice, T-cell-specific Wnt10b null mice, bone marrow transplantation assays, HSPC flow cytometry Blood Medium 23954891
2021 PTEN reduces BMP9-induced Wnt10b expression in mesenchymal stem cells through disrupting interaction between CREB and BMP/Smad1/5/9 signaling; p-CREB and p-Smad1/5/9 interact and are enriched at the Wnt10b promoter; PI3K inhibition (Ly294002) and rapamycin both reduce BMP9-induced Wnt10b expression, placing Wnt10b downstream of PI3K/mTOR and BMP/Smad signaling. Co-immunoprecipitation of CREB-Smad1/5/9, ChIP at Wnt10b promoter, PTEN overexpression/knockdown, PI3K and mTOR inhibitors, ALP activity and mineralization assays, in vivo bone mass assay Frontiers in cell and developmental biology Medium 33614622
2011 Leucine-rich Amelogenin Peptide (LRAP) promotes osteogenesis and inhibits adipogenesis of MSCs by upregulating Wnt10b expression to activate canonical Wnt/β-catenin signaling; Wnt10b siRNA knockdown abrogates LRAP's effects on MSC fate. LRAP treatment of ST2 cells, Wnt10b siRNA knockdown, sFRP-1 Wnt inhibition, Wnt/β-catenin reporter assays, osteogenic/adipogenic differentiation assays Biomaterials Medium 21663957
2006 Purified recombinant Wnt10b protein promotes differentiation of primary skin epithelial cells toward hair shaft and inner root sheath identity, inducing expression of keratin 1, keratin 2, loricrin, mHa5, mHb5, and reducing keratin 5; this differentiation involves the canonical Wnt signaling pathway (TCF reporter activation). Recombinant Wnt10b protein purification from lymphocyte supernatant, primary skin epithelial cell culture, immunocytochemistry, RT-PCR for differentiation markers, TCF reporter (pTOPFLASH) assay Biochemical and biophysical research communications Medium 16510119
2012 FHL2 silencing reduces Wnt10b expression in mesenchymal cells, and Wnt10b overexpression overcomes the negative effect of FHL2 knockdown on osteoblast gene expression; FHL2 knockout mice show decreased Wnt5a and Wnt10b expression in bone marrow with reduced bone mass and osteoblast function. FHL2 shRNA knockdown, Wnt10b overexpression rescue, FHL2 knockout mice, histomorphometry, qRT-PCR of Wnt molecules and osteoblast genes Bone Medium 23201222

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Regulation of osteoblastogenesis and bone mass by Wnt10b. Proceedings of the National Academy of Sciences of the United States of America 717 15728361
2018 The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression. Immunity 422 30446387
2011 Wnt6, Wnt10a and Wnt10b inhibit adipogenesis and stimulate osteoblastogenesis through a β-catenin-dependent mechanism. Bone 359 21872687
2004 Wnt10b inhibits development of white and brown adipose tissues. The Journal of biological chemistry 296 15190075
2007 Wnt10b increases postnatal bone formation by enhancing osteoblast differentiation. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 230 17708715
2013 WNT10B/β-catenin signalling induces HMGA2 and proliferation in metastatic triple-negative breast cancer. EMBO molecular medicine 152 23307470
2010 Wnt10b deficiency results in age-dependent loss of bone mass and progressive reduction of mesenchymal progenitor cells. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 150 20499361
2007 Wnt10b inhibits obesity in ob/ob and agouti mice. Diabetes 144 17259372
2009 T lymphocytes amplify the anabolic activity of parathyroid hormone through Wnt10b signaling. Cell metabolism 143 19723499
2005 Wnt10b deficiency promotes coexpression of myogenic and adipogenic programs in myoblasts. Molecular biology of the cell 131 15673614
1997 Wnt-10b directs hypermorphic development and transformation in mammary glands of male and female mice. Oncogene 128 9393971
1995 Murine Wnt-11 and Wnt-12 have temporally and spatially restricted expression patterns during embryonic development. Mechanisms of development 123 7547479
2015 Loss of Bone and Wnt10b Expression in Male Type 1 Diabetic Mice Is Blocked by the Probiotic Lactobacillus reuteri. Endocrinology 119 26135835
2004 Identification of the mammary line in mouse by Wnt10b expression. Developmental dynamics : an official publication of the American Association of Anatomists 113 14745960
2012 Silencing of miR-148a in cancer-associated fibroblasts results in WNT10B-mediated stimulation of tumor cell motility. Oncogene 107 22890324
2006 WNT10B mutations in human obesity. Diabetologia 107 16477437
2017 Aberrant low expression of p85α in stromal fibroblasts promotes breast cancer cell metastasis through exosome-mediated paracrine Wnt10b. Oncogene 106 28394344
2009 Activation of Wnt/beta-catenin signaling increases insulin sensitivity through a reciprocal regulation of Wnt10b and SREBP-1c in skeletal muscle cells. PloS one 102 20041157
2012 Adenovirus-mediated Wnt10b overexpression induces hair follicle regeneration. The Journal of investigative dermatology 87 22832493
1996 Murine Wnt10a and Wnt10b: cloning and expression in developing limbs, face and skin of embryos and in adults. Oncogene 87 8875992
2017 Exosomes Mediate Mobilization of Autocrine Wnt10b to Promote Axonal Regeneration in the Injured CNS. Cell reports 86 28683327
2011 The role of WNT10B in physiology and disease. Acta physiologica (Oxford, England) 84 21447090
2016 Mutations in WNT10B Are Identified in Individuals with Oligodontia. American journal of human genetics 81 27321946
2003 Wnt1 and wnt10b function redundantly at the zebrafish midbrain-hindbrain boundary. Developmental biology 76 12591239
1997 A novel human Wnt gene, WNT10B, maps to 12q13 and is expressed in human breast carcinomas. Oncogene 74 9121776
2001 Proto-oncogene WNT10B is up-regulated by tumor necrosis factor alpha in human gastric cancer cell line MKN45. International journal of oncology 72 11713588
2011 Wnt10b promotes growth of hair follicles via a canonical Wnt signalling pathway. Clinical and experimental dermatology 67 21392083
2008 Homozygous WNT10b mutation and complex inheritance in Split-Hand/Foot Malformation. Human molecular genetics 65 18515319
2013 TGF-β induces Wnt10b in osteoclasts from female mice to enhance coupling to osteoblasts. Endocrinology 64 23861379
2018 MiR-148a suppressed cell invasion and migration via targeting WNT10b and modulating β-catenin signaling in cisplatin-resistant colorectal cancer cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 63 30551544
2014 Modulating hair follicle size with Wnt10b/DKK1 during hair regeneration. Experimental dermatology 61 24750467
2018 The WNT10B Network Is Associated with Survival and Metastases in Chemoresistant Triple-Negative Breast Cancer. Cancer research 59 30563890
2014 The sclerostin-independent bone anabolic activity of intermittent PTH treatment is mediated by T-cell-produced Wnt10b. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 58 24357520
2008 Wnt10b induces chemotaxis of osteosarcoma and correlates with reduced survival. Pediatric blood & cancer 58 18465804
2004 Immunolocalization of BMP-2/-4, FGF-4, and WNT10b in the developing mouse first lower molar. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 55 14688221
2019 Coactivation of Endogenous Wnt10b and Foxc2 by CRISPR Activation Enhances BMSC Osteogenesis and Promotes Calvarial Bone Regeneration. Molecular therapy : the journal of the American Society of Gene Therapy 54 31882321
2015 Wnt10b Gain-of-Function Improves Cardiac Repair by Arteriole Formation and Attenuation of Fibrosis. Circulation research 53 26338900
2019 The balance of Bmp6 and Wnt10b regulates the telogen-anagen transition of hair follicles. Cell communication and signaling : CCS 52 30791955
2019 CRISPR/Cas9-mediated knockout of NSD1 suppresses the hepatocellular carcinoma development via the NSD1/H3/Wnt10b signaling pathway. Journal of experimental & clinical cancer research : CR 49 31727171
2014 CTLA-4Ig-induced T cell anergy promotes Wnt-10b production and bone formation in a mouse model. Arthritis & rheumatology (Hoboken, N.J.) 46 24757150
2017 MicroRNA-148a suppresses epithelial-mesenchymal transition and invasion of pancreatic cancer cells by targeting Wnt10b and inhibiting the Wnt/β-catenin signaling pathway. Oncology reports 44 28586066
2007 Wnt-10b, uniquely among Wnts, promotes epithelial differentiation and shaft growth. Biochemical and biophysical research communications 43 18155657
2011 Wnt10b activates the Wnt, notch, and NFκB pathways in U2OS osteosarcoma cells. Journal of cellular biochemistry 42 21321991
2002 Expression and regulation of WNT10B in human cancer: up-regulation of WNT10B in MCF-7 cells by beta-estradiol and down-regulation of WNT10B in NT2 cells by retinoic acid. International journal of molecular medicine 42 12239602
2020 Calcitonin Induces Bone Formation by Increasing Expression of Wnt10b in Osteoclasts in Ovariectomy-Induced Osteoporotic Rats. Frontiers in endocrinology 39 33013696
2020 Wnt10b-overexpressing umbilical cord mesenchymal stem cells promote critical size rat calvarial defect healing by enhanced osteogenesis and VEGF-mediated angiogenesis. Journal of orthopaedic translation 38 32477867
2019 Sinusoidal Electromagnetic Fields Increase Peak Bone Mass in Rats by Activating Wnt10b/β-Catenin in Primary Cilia of Osteoblasts. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 38 30779853
2018 WNT10B mutations associated with isolated dental anomalies. Clinical genetics 38 29364501
2011 A novel homozygous missense mutation in WNT10B in familial split-hand/foot malformation. Clinical genetics 38 21554266
2020 Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits. Bioscience reports 35 31961392
2010 Homozygous nonsense mutation in WNT10B and sporadic split-hand/foot malformation (SHFM) with autosomal recessive inheritance. American journal of medical genetics. Part A 35 20635353
2014 Oleuropein attenuates visceral adiposity in high-fat diet-induced obese mice through the modulation of WNT10b- and galanin-mediated signalings. Molecular nutrition & food research 34 25104077
2009 Association analysis of WNT10B with bone mass and structure among individuals of African ancestry. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 34 19016593
2007 Ectopic expression of Wnt10b decreases adiposity and improves glucose homeostasis in obese rats. American journal of physiology. Endocrinology and metabolism 34 17578883
2011 The influence of Leucine-rich amelogenin peptide on MSC fate by inducing Wnt10b expression. Biomaterials 33 21663957
2007 WNT10B functional dualism: beta-catenin/Tcf-dependent growth promotion or independent suppression with deregulated expression in cancer. Molecular biology of the cell 33 17761539
2003 Role of Wnt10b and C/EBPalpha in spontaneous adipogenesis of 243 cells. Biochemical and biophysical research communications 33 12593840
2012 Expression and the clinical significance of Wnt10a and Wnt10b in endometrial cancer are associated with the Wnt/β-catenin pathway. Oncology reports 32 23135473
2012 The LIM-only protein FHL2 controls mesenchymal cell osteogenic differentiation and bone formation through Wnt5a and Wnt10b. Bone 32 23201222
2006 Wnt-10b promotes differentiation of skin epithelial cells in vitro. Biochemical and biophysical research communications 32 16469292
2015 Downregulation of Microrna-148a in Cancer-Associated Fibroblasts from Oral Cancer Promotes Cancer Cell Migration and Invasion by Targeting Wnt10b. Journal of biochemical and molecular toxicology 30 26709120
2013 Hypoxia-inducible factor-2α-dependent hypoxic induction of Wnt10b expression in adipogenic cells. The Journal of biological chemistry 30 23900840
2023 The role of WNT10B in physiology and disease: A 10-year update. Frontiers in cell and developmental biology 29 36814601
2022 BMI1 promotes spermatogonial stem cell maintenance by epigenetically repressing Wnt10b/β-catenin signaling. International journal of biological sciences 29 35541907
2013 Ovariectomy expands murine short-term hemopoietic stem cell function through T cell expressed CD40L and Wnt10B. Blood 29 23954891
1996 The mouse Wnt-10B gene isolated from helper T cells is widely expressed and a possible oncogene in BR6 mouse mammary tumorigenesis. Gene 29 8682303
2016 CD8+ T cells mediate the regenerative PTH effect in hPDL cells via Wnt10b signaling. Innate immunity 28 28071181
2013 Canonical Wnts, specifically Wnt-10b, show ability to maintain dermal papilla cells. Biochemical and biophysical research communications 27 23916705
2006 Wnt-10b secreted from lymphocytes promotes differentiation of skin epithelial cells. Biochemical and biophysical research communications 27 16510119
2016 miR-148a is a downstream effector of X-box-binding protein 1 that silences Wnt10b during adipogenesis of 3T3-L1 cells. Experimental & molecular medicine 26 27055562
2007 Expression of Wnt5A and Wnt10B in non-immortalized breast cancer cells. Oncology reports 26 17342334
2007 AP1- and NF-kappaB-binding sites conserved among mammalian WNT10B orthologs elucidate the TNFalpha-WNT10B signaling loop implicated in carcinogenesis and adipogenesis. International journal of molecular medicine 25 17334647
2016 Immune Modulation of the T Cell Response in Asthma through Wnt10b. American journal of respiratory cell and molecular biology 24 26436894
2020 Long Noncoding RNA HOXB-AS1 Is Upregulated in Endometrial Carcinoma and Sponged miR-149-3p to Upregulate Wnt10b. Technology in cancer research & treatment 23 33073693
2015 Expression of Wnt3a, Wnt10b, β-catenin and DKK1 in periodontium during orthodontic tooth movement in rats. Acta odontologica Scandinavica 23 26414930
2010 A functional promoter polymorphism -607G>C of WNT10B is associated with abdominal fat in Korean female subjects. The Journal of nutritional biochemistry 23 20579865
2022 Sinomenine hydrochloride suppresses the stemness of breast cancer stem cells by inhibiting Wnt signaling pathway through down-regulation of WNT10B. Pharmacological research 22 35413424
2015 WNT10B enhances proliferation through β-catenin and RAC1 GTPase in human corneal endothelial cells. The Journal of biological chemistry 22 26370090
2005 Identification and characterization of rat Wnt1 and Wnt10b genes in silico. International journal of oncology 22 15703844
2020 Wnt10b-GSK3β-dependent Wnt/STOP signaling prevents aneuploidy in human somatic cells. Life science alliance 21 33257473
2017 MAT2A promotes porcine adipogenesis by mediating H3K27me3 at Wnt10b locus and repressing Wnt/β-catenin signaling. Biochimica et biophysica acta. Molecular and cell biology of lipids 21 29133280
2023 Wnt10b regulates osteogenesis of adipose-derived stem cells through Wnt/β-catenin signalling pathway in osteoporosis. Cell proliferation 20 37340715
2021 PTEN Reduces BMP9-Induced Osteogenic Differentiation Through Inhibiting Wnt10b in Mesenchymal Stem Cells. Frontiers in cell and developmental biology 20 33614622
2020 LncRNA-PCAT1 maintains characteristics of dermal papilla cells and promotes hair follicle regeneration by regulating miR-329/Wnt10b axis. Experimental cell research 20 32339605
2012 ApoA1: mimetic peptide reverses adipocyte dysfunction in vivo and in vitro via an increase in heme oxygenase (HO-1) and Wnt10b. Cell cycle (Georgetown, Tex.) 20 22306989
2018 Homozygous sequence variants in the WNT10B gene underlie split hand/foot malformation. Genetics and molecular biology 19 29384555
2018 Effect of Human Wnt10b Transgene Overexpression on Peri-Implant Osteogenesis in Ovariectomized Rats. Human gene therapy 19 29790378
2017 Wnt10B is critical for the progression of gastric cancer. Oncology letters 19 28599424
2015 Treatment with intermittent PTH increases Wnt10b production by T cells in osteoporotic patients. Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA 19 26068297
2013 X-box binding protein 1 enhances adipogenic differentiation of 3T3-L1 cells through the downregulation of Wnt10b expression. FEBS letters 19 23603388
1997 Isolation, characterization and chromosomal localization of human WNT10B. Cytogenetics and cell genetics 19 9284937
2019 The role of WNT10B in normal prostate gland development and prostate cancer. The Prostate 18 31433503
2015 Prolonged overexpression of Wnt10b induces epidermal keratinocyte transformation through activating EGF pathway. Histochemistry and cell biology 18 25995040
2013 Novel homozygous mutations in the WNT10B gene underlying autosomal recessive split hand/foot malformation in three consanguineous families. Gene 18 24211389
2023 Chelerythrine chloride inhibits the progression of colorectal cancer by targeting cancer-associated fibroblasts through intervention with WNT10B/β-catenin and TGFβ2/Smad2/3 axis. Phytotherapy research : PTR 17 37402476
2013 Associations of TCF12, CTNNAL1 and WNT10B gene polymorphisms with litter size in pigs. Animal reproduction science 17 23820070
2009 Polymorphisms of the WNT10B gene, bone mineral density, and fractures in postmenopausal women. Calcified tissue international 17 19458884
2020 lncRNA HOTAIRM1 regulates cell proliferation and the metastasis of thyroid cancer by targeting Wnt10b. Oncology reports 16 33650656
2018 FABP4 accelerates glioblastoma cell growth and metastasis through Wnt10b signalling. European review for medical and pharmacological sciences 16 30536325
2017 Low-level laser treatment stimulates hair growth via upregulating Wnt10b and β-catenin expression in C3H/HeJ mice. Lasers in medical science 16 28508243

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