Affinage

WASF1

Actin-binding protein WASF1 · UniProt Q92558

Length
559 aa
Mass
61.7 kDa
Annotated
2026-06-11
100 papers in source corpus 31 papers cited in narrative 31 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

WASF1 (WAVE1) is an actin-regulatory scaffold that drives Arp2/3-complex-dependent actin nucleation at sites of membrane protrusion, kept inactive within a heterotetrameric complex with PIR121, Nap125, and HSPC300 until Rac1 and the adapter Nck release an active WAVE1-HSPC300 dimer (PMID:12181570, PMID:19206172). Its output is tightly gated by inhibitory phosphorylation: Cdk5 phosphorylates Ser310/Ser397/Ser441 to suppress WAVE1-dependent actin polymerization, and these sites are dephosphorylated by PP2A and PP2B (calcineurin) downstream of D1 dopamine and NMDA receptor signaling to reactivate the protein (PMID:16862120, PMID:20403076). Beyond nucleating actin, WAVE1 serves as an A-kinase anchoring protein assembling a PKA/Abl kinase scaffold that translocates to sites of actin reorganization (PMID:10970852), and it is recruited to membranes through partners including CRMP-2/kinesin-1 for axonal transport, retrolinkin/CYFIP for BDNF-driven TrkB endocytosis, and NYAP/PI3K signaling (PMID:16260607, PMID:27605705, PMID:21946561). In the nervous system WAVE1 controls dendritic spine morphogenesis, synaptic plasticity, mitochondrial trafficking into spines, and oligodendrocyte myelination, with knockout mice showing learning and memory deficits (PMID:16862120, PMID:17215396, PMID:18287015, PMID:16723544, PMID:12578964). WAVE1 also functions in distinct cell types — platelet GPVI-collagen cytoskeletal responses, macrophage phagocytosis suppression via anchored PKA, and oocyte/embryonic nuclear transcriptional reprogramming through its WHD domain (PMID:17319906, PMID:23934128, PMID:23990560). De novo truncating mutations disrupting its C-terminal WCA actin-binding domain cause intellectual disability with seizures (PMID:29961568).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 2000 High

    Established that WAVE1 is not merely an actin effector but an organizing scaffold, by showing it anchors PKA and Abl kinase and relocates to actin-remodeling sites upon growth factor stimulation.

    Evidence AKAP screen and reciprocal Co-IP from brain extracts and HEK-293 cells with live-cell imaging in fibroblasts

    PMID:10970852

    Open questions at the time
    • Did not resolve how kinase anchoring is coordinated with actin nucleation
    • Competition between PKA-binding and actin-binding regions inferred from overlap, not directly tested functionally
  2. 2002 High

    Defined the core regulatory logic: WAVE1 is held inactive in a heterotetramer and released by Rac1/Nck to drive Arp2/3-dependent actin nucleation, answering how upstream GTPase signaling is converted to actin output.

    Evidence Biochemical purification, reconstitution, in vitro actin nucleation assays, and MS identification of PIR121/Nap125/HSPC300

    PMID:12181570

    Open questions at the time
    • Did not establish in vivo physiological triggers in specific cell types
    • Structural basis of WCA masking not resolved
  3. 2003 High

    Distinguished WAVE1 from WAVE2 functionally, showing WAVE1 specifically drives dorsal (not peripheral) ruffles and couples to MMP-2 for ECM migration, and that WAVE1-null mice have neurobehavioral deficits.

    Evidence Knockout MEFs with PDGF stimulation and MMP inhibition, plus targeted KO mouse behavioral testing

    PMID:12578964 PMID:14536061

    Open questions at the time
    • Molecular basis of WAVE1 vs WAVE2 specificity not defined
    • Link between cellular ruffle defect and behavioral phenotype not mechanistically connected
  4. 2006 High

    Identified the dominant post-translational switch controlling WAVE1: Cdk5 phosphorylation at Ser310/397/441 inhibits actin polymerization and constrains dendritic spine maturation, with phospho-mimic mutants failing to rescue.

    Evidence In vitro Cdk5 kinase assay, phospho-/dephospho-mimic mutagenesis, neuron morphology, and in vivo mouse studies

    PMID:16862120

    Open questions at the time
    • Did not identify the phosphatases reversing these sites
    • Receptor signaling upstream of Cdk5 site control left open
  5. 2007 High

    Placed WAVE1 within synaptic and hematologic pathways, defining a WRP-RhoGAP partnership required for spine density/cognition and a GPVI-collagen-specific role in platelet lamellipodia.

    Evidence Gene targeting disrupting WRP-WAVE1 interaction with electrophysiology/behavior; WAVE1-KO platelet substrate-specific functional assays

    PMID:17215396 PMID:17319906

    Open questions at the time
    • How WRP GAP activity feeds back onto Rac1/WAVE1 not fully resolved
    • Receptor-proximal signaling linking GPVI to WAVE1 not mapped
  6. 2008 Medium

    Connected receptor activity to WAVE1 activation in neurons, showing NMDA-receptor-driven p35 degradation lowers Cdk5-site phosphorylation and licenses mitochondrial movement into spines.

    Evidence Neuronal depolarization assays, NMDA pharmacology, and mitochondrial trafficking imaging

    PMID:18287015

    Open questions at the time
    • Direct mechanistic link between WAVE1 actin activity and mitochondrial transport machinery not established
    • Single-lab functional imaging
  7. 2009 Medium

    Confirmed the native human WAVE complex is intrinsically inactive (WCA masked), reinforcing membrane recruitment as the required activation step, and extended WAVE1 to apoptotic and signaling scaffolding roles.

    Evidence Purified native complex actin assays; estradiol pathway dissection; WAVE1-Bcl-2 Co-IP and RNAi in leukemia cells

    PMID:19206172 PMID:19460862 PMID:19890377

    Open questions at the time
    • Mitochondrial Bcl-2/apoptotic role mechanistically distinct from actin function and not unified
    • Estradiol cascade single-lab
  8. 2010 Medium

    Completed the phospho-switch model by identifying PP2A and PP2B as the site-specific phosphatases that reverse Cdk5 phosphorylation under D1-dopamine and NMDA receptor signaling.

    Evidence In vitro phosphatase assays and receptor pharmacology in neurons

    PMID:20403076

    Open questions at the time
    • Quantitative contribution of each phosphatase in vivo unresolved
    • Single lab
  9. 2011 High

    Expanded the recruitment logic, showing NYAP phosphoproteins bridge PI3K to the WAVE1 complex downstream of Contactin5/Fyn to control neuronal morphogenesis.

    Evidence Co-IP, tyrosine phosphorylation and PI3K activity assays, and NYAP KO mice

    PMID:21946561

    Open questions at the time
    • Whether PI3K recruitment is additive to or competes with Rac1 activation not addressed
  10. 2013 High

    Revealed an unexpected nuclear function: Wave1 acts in the oocyte nucleus via its WHD domain to support RNA Pol II activity and transcriptional reprogramming of transplanted nuclei.

    Evidence Xenopus oocyte microinjection, knockdown, domain-mapping Co-IP, and Hox activation readouts; macrophage AKAP-PKA phagocytosis dissection

    PMID:23934128 PMID:23990560

    Open questions at the time
    • How an actin scaffold supports transcription mechanistically not resolved
    • Nuclear and cytoplasmic pools not reconciled
  11. 2015 High

    Linked WAVE1 to disease-relevant trafficking and to APP/Aβ biology, showing WAVE1 promotes APP vesicle budding and that lowering WAVE1 reduces Aβ and rescues memory in an AD model.

    Evidence ChIP of AICD at Wasf1 promoter, Golgi colocalization, APP budding assays, and in vivo AD model rescue; CB1 receptor WAVE1-complex proteomics

    PMID:26280122 PMID:26496209

    Open questions at the time
    • Whether WAVE1's actin-nucleation activity per se drives APP budding not isolated
    • CB1-WAVE1 actin collapse mechanism only partly defined
  12. 2018 Medium

    Established direct human disease causation, showing de novo truncating WASF1 mutations disrupting the C-terminal WCA domain produce defective actin remodeling and cause intellectual disability with seizures.

    Evidence Exome/genome sequencing with Western blot and actin remodeling assays in patient fibroblasts

    PMID:29961568

    Open questions at the time
    • Single study
    • Genotype-phenotype range and dominant-negative vs haploinsufficiency mechanism not resolved
  13. 2021 High

    Uncovered an Arp2/3-independent morphogenetic role, showing the WAVE complex localizes to saddle membrane curvature as actin-independent rings and supports protrusions even in ARP2-null cells.

    Evidence Super-resolution microscopy with ARP2-null and WAVE-complex-null cells and CRISPR KO actin dynamics measurements

    PMID:32697617 PMID:34096975

    Open questions at the time
    • Mechanism by which the complex senses curvature not defined
    • Functional separation of Arp2/3-dependent and -independent activities incomplete

Open questions

Synthesis pass · forward-looking unresolved questions
  • How WAVE1's diverse non-canonical roles — nuclear transcription, mitochondrial apoptotic complexes, and curvature sensing — relate to its core Arp2/3-activating actin function remains unresolved.
  • No unified structural model linking WCA masking, AKAP scaffolding, and nuclear/mitochondrial pools
  • Disease mechanism (dominant-negative vs loss-of-function) not definitively established

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 5 GO:0008092 cytoskeletal protein binding 3 GO:0098772 molecular function regulator activity 3
Localization
GO:0005886 plasma membrane 5 GO:0005739 mitochondrion 4 GO:0005856 cytoskeleton 4 GO:0005634 nucleus 2 GO:0005794 Golgi apparatus 2
Pathway
R-HSA-112316 Neuronal System 5 R-HSA-162582 Signal Transduction 4 R-HSA-5357801 Programmed Cell Death 2 R-HSA-5653656 Vesicle-mediated transport 2 R-HSA-74160 Gene expression (Transcription) 2
Partners
ABL1BCL2CRMP2/DPYSL2CYFIP1KLC/KINESIN-1NCKAP1PRKACARAC1
Complex memberships
WAVE regulatory complex (WAVE1-PIR121/CYFIP-Nap125-HSPC300)

Evidence

Reading pass · 31 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2002 WAVE1 exists in a heterotetrameric complex with PIR121, Nap125, and HSPC300 that is constitutively inactive; Rac1 and the adapter protein Nck cause dissociation of this complex, releasing active WAVE1-HSPC300 to drive actin nucleation via the Arp2/3 complex. Biochemical purification, reconstitution, in vitro actin nucleation assays, mass spectrometry identification of complex components Nature High 12181570
2000 WAVE-1 acts as an A-kinase anchoring protein (AKAP) that assembles a multi-kinase scaffold by simultaneously binding cAMP-dependent protein kinase (PKA) and Abelson tyrosine kinase (Abl); the PKA-binding site overlaps a verprolin homology region that interacts with actin, suggesting competitive regulation. The scaffold dynamically translocates to sites of actin reorganization upon PDGF stimulation. AKAP screen, Co-immunoprecipitation from HEK-293 cells and brain extracts, mapping studies, immunocytochemistry in Swiss 3T3 fibroblasts The EMBO journal High 10970852
2006 Cyclin-dependent kinase 5 (Cdk5) phosphorylates WAVE1 at multiple serine residues (Ser310, Ser397, Ser441) in vitro and in intact mouse neurons, and phosphorylation inhibits WAVE1-dependent Arp2/3 complex-mediated actin polymerization. Loss of WAVE1 function reduces mature dendritic spine density; a dephosphorylation-mimic mutant rescues spine morphology but a phosphorylation-mimic mutant does not. cAMP signaling reduces Cdk5-site phosphorylation and increases spine density in a WAVE1-dependent manner. In vitro kinase assay with Cdk5, phosphorylation-mimic and dephosphorylation-mimic mutagenesis, actin polymerization assays, cultured neuron morphology analysis, in vivo mouse studies Nature High 16862120
2005 CRMP-2 interacts with the Sra-1/WAVE1 complex and links it to kinesin-1 light chain, mediating anterograde transport of the Sra-1/WAVE1 complex to axonal growth cones. Knockdown of Sra-1 and WAVE1 canceled CRMP-2-induced axon outgrowth and multiple-axon formation; knockdown of CRMP-2 or kinesin-1 delocalized Sra-1 and WAVE1 from growth cone tips. Co-immunoprecipitation, RNA interference knockdown, immunofluorescence localization in hippocampal neurons Molecular and cellular biology High 16260607
2003 WAVE2 deficiency impairs peripheral ruffle formation, while WAVE1 deficiency specifically impairs dorsal ruffle formation in PDGF-stimulated fibroblasts. WAVE1, but not WAVE2, colocalizes with matrix metalloproteinase MMP-2 in dorsal ruffles, and WAVE1-dependent migration in ECM requires MMP activity. Knockout mouse embryonic fibroblasts, PDGF stimulation assays, immunofluorescence colocalization with MMP-2, MMP inhibitor experiments Developmental cell High 14536061
2007 WAVE-1 forms a signaling complex with the GTPase-activating protein WRP (WAVE-associated RhoGAP protein); WRP anchoring to WAVE-1 is required for normal dendritic spine density, synaptic plasticity, and cognitive behavior in mice, as shown by gene targeting that disrupts WRP–WAVE-1 interaction. Gene targeting in mice, neuronal time-lapse imaging, electrophysiological recordings, behavioral analyses The Journal of neuroscience High 17215396
2006 WAVE1 is expressed in oligodendrocytes and localizes to the lamella leading edge; dominant-negative WAVE1 impairs process outgrowth and lamellipodia formation in oligodendrocytes. WAVE1-knockout mice exhibit regional hypomyelination in corpus callosum and optic nerve, with fewer nodes of Ranvier, demonstrating a cell-autonomous role for WAVE1 in oligodendrocyte morphogenesis and CNS myelination. Dominant-negative expression, WAVE1-knockout mouse analysis, immunofluorescence localization, electron microscopy of myelin The Journal of neuroscience High 16723544
2003 WAVE1 knockout mice display sensorimotor retardation, reduced anxiety, and deficits in hippocampal-dependent learning and memory, establishing that WAVE-1 is required for normal neural functioning and implicating it in the WAVE-1 signaling network relevant to mental retardation phenotypes. Targeted gene disruption (knockout) in mice, behavioral testing, hippocampal-dependent memory assays Proceedings of the National Academy of Sciences of the United States of America High 12578964
2008 WAVE1 controls depolarization-induced mitochondrial movement into dendritic spines and filopodia. NMDA receptor activation causes degradation of the p35 regulatory subunit of Cdk5, reducing inhibitory phosphorylation of WAVE1, and this dephosphorylation is associated with mitochondrial redistribution and spine morphogenesis. Neuronal depolarization assays, WAVE1 expression manipulation, NMDA receptor pharmacology, mitochondrial trafficking imaging Proceedings of the National Academy of Sciences of the United States of America Medium 18287015
2009 The purified native human WAVE complex is intrinsically inactive in actin nucleation assays, indicating that the WCA domain is masked at resting state, analogous to WASP proteins; the complex must be recruited and activated at the plasma membrane to drive lamellipodia formation. Affinity purification of native WAVE complex from stable human cell line, in vitro Arp2/3-dependent actin polymerization assay Cell motility and the cytoskeleton High 19206172
2013 Xenopus Wave1 is present in the oocyte nucleus and is required for transcriptional reprogramming of sperm and somatic nuclei. Nuclear Wave1 binds active transcription components via its WHD domain and contributes to RNA polymerase II activity. Wave1 knockdown in embryos causes abnormal development and defective Hox gene activation. Xenopus oocyte microinjection, knockdown experiments, co-immunoprecipitation of Wave1 with transcription components, domain mapping Science High 23990560
2003 VEGF stimulation causes WAVE1 to bind p47phox within membrane ruffles; WAVE1 acts as a scaffold recruiting the NADPH oxidase to a complex with Rac1 and PAK1. PAK1 kinase activity is required for p47phox phosphorylation, oxidant production, and ruffle formation. WAVE1 domain-based competition experiments block VEGF-induced ruffling. Co-immunoprecipitation, dominant-negative domain expression, NADPH oxidase activity assays, ruffle formation assays in endothelial cells The Journal of biological chemistry Medium 12855698
2009 Estradiol (E2) rapidly phosphorylates WAVE1 at Ser310, Ser397, and Ser441 in rat cortical neurons via a Gαi/Gβ → c-Src → Rac1 → Cdk5 signaling cascade, causing WAVE1 redistribution to the cell membrane at sites of dendritic spine formation and triggering local Arp2/3 complex concentration and actin reorganization. Silencing WAVE1 abrogates E2-induced spine increase. Phospho-specific antibodies, signaling pathway inhibitors, Rac1/Cdk5 knockdown, immunofluorescence in cortical neurons, spine counting Molecular endocrinology Medium 19460862
2007 WAVE1 and pancortin-2 form a protein complex with Bcl-xL at mitochondria in adult cortical neurons following ischemic stroke; this three-protein complex increases Bax mitochondrial association, cytochrome c release, and neuronal apoptosis. In pancortin-null mice, WAVE1–Bcl-xL interaction is diminished and cortical neurons are protected against ischemia. Co-immunoprecipitation, subcellular fractionation, pancortin conditional knockout (Cre-loxP), focal ischemia model in mice The Journal of neuroscience High 17301160
2009 WAVE1 interacts with mitochondrial Bcl-2 in leukemia cells; depletion of WAVE1 by RNAi causes mitochondrial release of Bcl-2, phosphorylation of ASK1/JNK and Bcl-2, and increases ROS production, leading to increased apoptosis. WAVE1 overexpression confers resistance to drug-induced apoptosis. RNA interference, gene transfection, Co-immunoprecipitation, apoptosis assays, ROS measurement Leukemia Medium 19890377
2015 The APP intracellular domain (AICD) binds the Wasf1 promoter and negatively regulates WAVE1 transcription. WAVE1 colocalizes with APP in the Golgi apparatus; reducing WAVE1 decreases budding of APP-containing vesicles, reduces cell-surface APP, and thereby lowers Aβ production. WAVE1 reduction in a mouse AD model dramatically reduced Aβ levels and restored memory deficits. Chromatin immunoprecipitation (AICD at Wasf1 promoter), Golgi colocalization, APP vesicle budding assays, APP/Aβ ELISA, mouse AD model behavior Nature medicine High 26280122
2015 The cannabinoid CB1 receptor assembles with multiple members of the WAVE1 complex and with Rac1 in neurons in vivo; CB1 receptor activation modulates actin polymerization and stability via WAVE1 in growth cones and synaptic spines, causing collapse/retraction. CB1 agonists attenuate activity-dependent dendritic spine remodeling in spinal cord neurons in vivo via the WAVE1 complex. In vivo proteomics pull-down from mouse cortex, actin polymerization assays, dendritic spine imaging in vivo, pain behavior assays PLoS biology High 26496209
2011 The NYAP family of phosphoproteins (NYAP1, NYAP2, Myosin16/NYAP3) are tyrosine-phosphorylated by Fyn upon Contactin5 stimulation; phosphorylated NYAPs activate PI3K and simultaneously interact with the WAVE1 complex, physically bridging a PI3K–WAVE1 association that regulates actin cytoskeleton remodeling and neuronal morphogenesis. Co-immunoprecipitation, tyrosine phosphorylation assays, PI3K activity assays, NYAP gene disruption in mice The EMBO journal High 21946561
2010 WAVE1 phosphorylation at Ser310, Ser397, and Ser441 (by Cdk5) can be reversed by protein phosphatase-2A (PP2A) and protein phosphatase-2B (PP2B/calcineurin), acting on different sites in response to D1 dopamine receptor (via cAMP) and NMDA receptor (via Ca2+) signaling respectively, thereby activating WAVE1. Phosphatase inhibitor studies, in vitro phosphatase assays, receptor pharmacology in neurons Journal of neurochemistry Medium 20403076
2004 WAVE1 undergoes cell-cycle-dependent nuclear localization during fertilization: redistributes from cortex in GV oocytes to cytoplasmic foci in Met II oocytes, then to pronuclei after sperm entry. Nuclear localization is independent of actin/Arp2/3 and nuclear pores but requires dynamic microtubules. Anti-WAVE1 antibodies prevent pronuclear migration. In Met II oocytes WAVE1 associates with PKA regulatory subunit but not Arp2/3; in Met II it also interacts with Arp2/3. Immunofluorescence, immunoprecipitation, wheat germ agglutinin microinjection, taxol microtubule stabilization, antibody microinjection Developmental biology Medium 15581863
2004 During mammalian spermatogenesis, WAVE1 localizes to the Golgi apparatus in spermatocytes/round spermatids and to the mitochondrial sheath in elongated spermatids and sperm. WAVE1 co-localizes with PKA RII along the mitochondrial sheath, establishing a signaling unit in the sperm midpiece; this distribution is conserved across mouse, bull, baboon, and human. Transmission electron microscopy, immunocytochemistry, western blotting across multiple species Human reproduction Medium 15471936
2007 WAVE1 plays a major role downstream of the GPVI collagen receptor in platelets: WAVE1-knockout platelets show markedly inhibited lamellipodia formation on CRP and laminin (GPVI-dependent substrates) and impaired aggregation in response to CRP, while responses to GPCR agonist thrombin are unaffected. WAVE1 knockout mouse platelets, lamellipodia formation assays on defined substrates, platelet aggregation assays Journal of thrombosis and haemostasis High 17319906
2013 WAVE1 (acting as an AKAP) mediates the suppression of macrophage phagocytosis by oxidized phospholipids (OxPL/DAMPs): OxPL activates an anchored PKA pool, and WAVE1 gene silencing or Wave1-knockout chimeric mice abolish OxPL-induced impairment of phagocytosis and extend survival after E. coli infection. RNAi gene silencing, Wave1-knockout chimeric mice, phagocytosis assays, in vivo bacterial infection model The Journal of clinical investigation High 23934128
2018 De novo heterozygous truncating mutations in WASF1 (WAVE1) that partially or fully disrupt the C-terminal WCA actin-binding domain cause intellectual disability with seizures. Functional studies in patient fibroblasts confirmed truncated WASF1 protein and a defect in actin remodeling. Exome/whole-genome sequencing of patients, Western blot for truncated protein in patient fibroblasts, actin remodeling assays in patient cells American journal of human genetics Medium 29961568
2016 Retrolinkin interacts with the CYFIP1/2 subunit of the WAVE1 complex; this interaction recruits WAVE1 to the neuronal plasma membrane upon BDNF stimulation and is required for clathrin-independent, actin-dependent endocytosis of activated TrkB and subsequent dendrite outgrowth. N-WASP is not required for this process. Co-immunoprecipitation, dominant-negative disruption of retrolinkin–CYFIP1/2 interaction, WAVE1 knockdown, TrkB endocytosis assays, dendrite morphology Molecular biology of the cell Medium 27605705
2005 WAVE1 is required for stabilization (but not initial extension) of lamellipodial protrusions during cell spreading on fibronectin: WAVE1-KO MEFs show decreased actin filament density at protrusion tips and increased membrane extension speed, causing focal complex deformation, while WAVE2-KO MEFs fail to form lamellipodial structures. Knockout MEFs (WAVE1-KO and WAVE2-KO), live-cell imaging during spreading on fibronectin, actin filament density analysis Genes to cells Medium 15836768
2009 ArgBP2 and CIP4 both directly interact with WAVE1 and can enhance its tyrosine phosphorylation catalyzed by c-Abl; ArgBP2 and CIP4 act synergistically to increase WAVE1 tyrosine phosphorylation. However, CIP4 is dispensable for ArgBP2-induced blockade of cell migration. Yeast two-hybrid, Co-immunoprecipitation, in vitro kinase assays with c-Abl, cell migration assays Cancer letters Medium 19631450
2004 WAVE1 isoform is preferentially precipitated by profilin I (SH3 domain pull-down) compared with WAVE2, while the IRSp53 SH3 domain precipitates WAVE2 more efficiently; the Abl SH3 domain binds all three WAVE isoforms. All three WAVE isoforms are substrates for calpain cleavage in vivo and in vitro in platelets. SH3 domain pull-down with platelet lysates, in vitro calpain cleavage assays, Triton X-100 fractionation Blood Medium 15280206
2020 WAVE1 and WAVE2 are redundant for lamellipodia formation and motility in B16-F1 melanoma cells; however, WAVE1 KO increases the rate of leading-edge actin extension (while WAVE2 KO decreases it), and WAVE1 restricts actin extension rate and couples actin networks to the membrane to drive protrusion. The faster actin extension in WAVE1-KO cells is offset by increased retrograde flow. CRISPR knockout of WAVE1 and WAVE2 individually and together, live-cell imaging of actin dynamics at leading edge, protrusion rate measurements Molecular biology of the cell High 32697617
2015 Phosphorylated WAVE1 (p-WAVE1) and phosphorylated CRMP2 co-localize with aggregated hyperphosphorylated tau in hippocampal neurons of triple-transgenic AD model mice and are present in sarkosyl-insoluble fractions; both are phosphorylated by Cdk5 and their phosphorylation correlates with tau aggregate formation. Immunofluorescence colocalization, subcellular fractionation (sarkosyl-insoluble), ATRA pharmacological treatment in 3xTg-AD mice Journal of neuroscience research Low 26400044
2021 The WAVE complex localizes to regions of saddle membrane curvature as actin-independent 230-nm-wide rings (visualized by super-resolution microscopy); the WAVE complex recruits IRSp53 to saddle curvature sites but does not depend on IRSp53 for its own localization. Sheet-like protrusions persist in ARP2-null cells but not in WAVE complex-null cells, indicating WAVE complex has roles in morphogenesis beyond Arp2/3 activation. Super-resolution microscopy, ARP2-null and WAVE complex-null cell lines, curvature analysis, IRSp53 localization The Journal of cell biology High 34096975

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2011 ROS signaling: the new wave? Trends in plant science 1394 21482172
2012 Engineering the third wave of biocatalysis. Nature 1304 22575958
2002 Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Nature 667 12181570
2013 Surface acoustic wave microfluidics. Lab on a chip 433 23900527
2009 J wave syndromes. Heart rhythm 394 20153265
2007 An actin-based wave generator organizes cell motility. PLoS biology 327 17696648
2020 TDP-43 proteinopathies: a new wave of neurodegenerative diseases. Journal of neurology, neurosurgery, and psychiatry 281 33177049
2003 Survivin study: what is the next wave? Journal of cellular physiology 275 12942537
2003 Differential roles of WAVE1 and WAVE2 in dorsal and peripheral ruffle formation for fibroblast cell migration. Developmental cell 263 14536061
2006 Phosphorylation of WAVE1 regulates actin polymerization and dendritic spine morphology. Nature 255 16862120
2017 Propagating Wave of ERK Activation Orients Collective Cell Migration. Developmental cell 222 29112851
2005 CRMP-2 is involved in kinesin-1-dependent transport of the Sra-1/WAVE1 complex and axon formation. Molecular and cellular biology 207 16260607
2004 Histone deacetylase inhibitors: understanding a new wave of anticancer agents. International journal of cancer 202 15352027
2006 Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes. Molecular biology of the cell 187 16597702
2018 Slow-wave sleep: From the cell to the clinic. Sleep medicine reviews 185 29490885
2003 An overview of shock wave therapy in musculoskeletal disorders. Chang Gung medical journal 180 12846521
2007 A WAVE-1 and WRP signaling complex regulates spine density, synaptic plasticity, and memory. The Journal of neuroscience : the official journal of the Society for Neuroscience 178 17215396
2000 Scar/WAVE-1, a Wiskott-Aldrich syndrome protein, assembles an actin-associated multi-kinase scaffold. The EMBO journal 175 10970852
2003 Loss of WAVE-1 causes sensorimotor retardation and reduced learning and memory in mice. Proceedings of the National Academy of Sciences of the United States of America 170 12578964
2006 Survivin study: an update of "what is the next wave"? Journal of cellular physiology 153 16557517
2005 The next wave in metabolome analysis. Trends in biotechnology 142 16154652
2018 TWEAK and RIPK1 mediate a second wave of cell death during AKI. Proceedings of the National Academy of Sciences of the United States of America 132 29588419
2006 Calcium wave of tubuloglomerular feedback. American journal of physiology. Renal physiology 120 16495210
2004 Transdermal drug delivery with a pressure wave. Advanced drug delivery reviews 120 15019746
2009 The WASP and WAVE family proteins. Genome biology 118 19589182
2010 Calcium wave signaling in cancer cells. Life sciences 116 20875431
2023 A new wave of innovations within the DNA damage response. Signal transduction and targeted therapy 114 37679326
2019 Genetic induction and mechanochemical propagation of a morphogenetic wave. Nature 111 31413363
2016 Heat Wave-Associated Vibriosis, Sweden and Finland, 2014. Emerging infectious diseases 111 27314874
2020 Extracorporeal shock wave therapy: an update. EFORT open reviews 107 33204500
2008 WAVE1 controls neuronal activity-induced mitochondrial distribution in dendritic spines. Proceedings of the National Academy of Sciences of the United States of America 101 18287015
2011 Involvement of cytokines in slow wave sleep. Progress in brain research 96 21854954
2021 WAVE regulatory complex. Current biology : CB 93 34033782
2006 WAVE1 is required for oligodendrocyte morphogenesis and normal CNS myelination. The Journal of neuroscience : the official journal of the Society for Neuroscience 86 16723544
2021 A flexible electromagnetic wave-electricity harvester. Nature communications 85 33547310
2018 Venetoclax: A new wave in hematooncology. Experimental hematology 81 29477371
2009 The Wave complex is intrinsically inactive. Cell motility and the cytoskeleton 80 19206172
2003 Characterization of the WAVE1 knock-out mouse: implications for CNS development. The Journal of neuroscience : the official journal of the Society for Neuroscience 80 12716942
2013 Nuclear Wave1 is required for reprogramming transcription in oocytes and for normal development. Science (New York, N.Y.) 77 23990560
2003 Vascular endothelial growth factor causes translocation of p47phox to membrane ruffles through WAVE1. The Journal of biological chemistry 75 12855698
2003 Kette regulates actin dynamics and genetically interacts with Wave and Wasp. Development (Cambridge, England) 75 12900458
2009 Rapid signaling of estrogen to WAVE1 and moesin controls neuronal spine formation via the actin cytoskeleton. Molecular endocrinology (Baltimore, Md.) 74 19460862
2020 Calcium Wave Promotes Cell Extrusion. Current biology : CB 73 32004455
2005 Regulation of actin assembly by SCAR/WAVE proteins. Biochemical Society transactions 73 16246088
2013 Phosphoregulation of the WAVE regulatory complex and signal integration. Seminars in cell & developmental biology 72 23354023
2003 Experience-dependent slow-wave sleep development. Nature neuroscience 72 12754515
2019 Wave patterns organize cellular protrusions and control cortical dynamics. Molecular systems biology 71 30858181
2018 The fourth wave of biocatalysis is approaching. Philosophical transactions. Series A, Mathematical, physical, and engineering sciences 70 29175831
2022 The next wave of cellular immunotherapies in pancreatic cancer. Molecular therapy oncolytics 67 35229033
2008 Expression of Aire and the early wave of apoptosis in spermatogenesis. Journal of immunology (Baltimore, Md. : 1950) 67 18209027
2015 The Cannabinoid Receptor CB1 Interacts with the WAVE1 Complex and Plays a Role in Actin Dynamics and Structural Plasticity in Neurons. PLoS biology 61 26496209
2011 NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons. The EMBO journal 55 21946561
2021 Driving DNA Origami Assembly with a Terahertz Wave. Nano letters 53 34968055
2016 Adhesion-Dependent Wave Generation in Crawling Cells. Current biology : CB 53 27939309
1986 New wave of research in the epilepsies. Advances in neurology 52 2871720
2012 Distributed computation: the new wave of synthetic biology devices. Trends in biotechnology 50 22516742
2007 Pancortin-2 interacts with WAVE1 and Bcl-xL in a mitochondria-associated protein complex that mediates ischemic neuronal death. The Journal of neuroscience : the official journal of the Society for Neuroscience 50 17301160
2007 Nutrigenetics and nutraceuticals: the next wave riding on personalized medicine. Translational research : the journal of laboratory and clinical medicine 46 17240315
2014 Mouse b-wave mutants. Documenta ophthalmologica. Advances in ophthalmology 45 24395437
2004 WAVE/Scars in platelets. Blood 45 15280206
2001 cAMP receptor affinity controls wave dynamics, geometry and morphogenesis in Dictyostelium. Journal of cell science 44 11559759
2016 Functional Coordination of WAVE and WASP in C. elegans Neuroblast Migration. Developmental cell 43 27780040
2011 Shock wave therapy in wound healing. Plastic and reconstructive surgery 43 21841528
2010 Kinetochores' gripping feat: conformational wave or biased diffusion? Trends in cell biology 43 20951587
2021 The WAVE complex associates with sites of saddle membrane curvature. The Journal of cell biology 42 34096975
2015 APP intracellular domain-WAVE1 pathway reduces amyloid-β production. Nature medicine 41 26280122
1995 Evanescent wave biosensors. Real-time analysis of biomolecular interactions. Molecular biotechnology 40 7606504
1998 Ca2+ wave dispersion and spiral wave entrainment in Xenopus laevis oocytes overexpressing Ca2+ ATPases. Biophysical chemistry 39 9652090
2009 WAVE1 regulates Bcl-2 localization and phosphorylation in leukemia cells. Leukemia 37 19890377
2006 WAVE signalling: from biochemistry to biology. Biochemical Society transactions 37 16417486
2004 WAVE1 intranuclear trafficking is essential for genomic and cytoskeletal dynamics during fertilization: cell-cycle-dependent shuttling between M-phase and interphase nuclei. Developmental biology 36 15581863
2019 Calibrating Evanescent-Wave Penetration Depths for Biological TIRF Microscopy. Biophysical journal 35 31439287
2018 De Novo Truncating Mutations in WASF1 Cause Intellectual Disability with Seizures. American journal of human genetics 34 29961568
2011 J-wave syndromes. from cell to bedside. Journal of electrocardiology 34 21908004
2005 A novel function of WAVE in lamellipodia: WAVE1 is required for stabilization of lamellipodial protrusions during cell spreading. Genes to cells : devoted to molecular & cellular mechanisms 34 15836768
2016 Shock Wave Enhances Angiogenesis through VEGFR2 Activation and Recycling. Molecular medicine (Cambridge, Mass.) 33 27925633
1995 Q wave and Non-Q wave myocardial infarction after thrombolysis. Journal of the American College of Cardiology 33 7594069
2010 Hem-1: putting the "WAVE" into actin polymerization during an immune response. FEBS letters 31 20969869
2009 Traveling wave formation in vertebrate segmentation. Journal of theoretical biology 31 19174170
2021 A traveling-wave solution for bacterial chemotaxis with growth. Proceedings of the National Academy of Sciences of the United States of America 30 34819366
2018 Shock wave-induced permeabilization of mammalian cells. Physics of life reviews 30 29685859
2012 Deterministic versus stochastic cell polarisation through wave-pinning. Bulletin of mathematical biology 30 22956290
2008 WAVE1 is associated with invasiveness and growth of prostate cancer cells. The Journal of urology 30 18710763
2004 WAVE1, an A-kinase anchoring protein, during mammalian spermatogenesis. Human reproduction (Oxford, England) 27 15471936
1998 Q wave and non-Q wave myocarditis with special reference to clinical significance. Japanese heart journal 26 10089938
2021 Effect of Radial Extracorporeal Shock Wave Combined With Ultrashort Wave Diathermy on Fibrosis and Contracture of Muscle. American journal of physical medicine & rehabilitation 25 32969968
2010 Signaling pathways controlling the phosphorylation state of WAVE1, a regulator of actin polymerization. Journal of neurochemistry 25 20403076
2007 A major role for Scar/WAVE-1 downstream of GPVI in platelets. Journal of thrombosis and haemostasis : JTH 25 17319906
2016 Retrolinkin recruits the WAVE1 protein complex to facilitate BDNF-induced TrkB endocytosis and dendrite outgrowth. Molecular biology of the cell 24 27605705
2013 WAVE1 mediates suppression of phagocytosis by phospholipid-derived DAMPs. The Journal of clinical investigation 24 23934128
2023 Intercellular transmission of pathogenic proteins in ALS: Exploring the pathogenic wave. Neurobiology of disease 23 37394036
2020 WAVE1 and WAVE2 have distinct and overlapping roles in controlling actin assembly at the leading edge. Molecular biology of the cell 23 32697617
2018 Guided wave elastography of layered soft tissues. Acta biomaterialia 23 30528611
2014 The ventral tegmentum and dopamine: A new wave of diversity. Neuroscience 22 25453764
2009 CIP4 is a new ArgBP2 interacting protein that modulates the ArgBP2 mediated control of WAVE1 phosphorylation and cancer cell migration. Cancer letters 22 19631450
1988 Morphology of spikes in spike-and-wave complexes. Electroencephalography and clinical neurophysiology 21 2453327
2020 Wave Propagation and Energy Dissipation in Collagen Molecules. ACS biomaterials science & engineering 20 33455394
2016 Knockdown of WAVE1 enhances apoptosis of leukemia cells by downregulating autophagy. International journal of oncology 19 27035872
2015 Colocalization of phosphorylated forms of WAVE1, CRMP2, and tau in Alzheimer's disease model mice: Involvement of Cdk5 phosphorylation and the effect of ATRA treatment. Journal of neuroscience research 19 26400044
2014 Biochemical reconstitution of the WAVE regulatory complex. Methods in enzymology 19 24630101

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