Affinage

VCAM1

Vascular cell adhesion protein 1 · UniProt P19320

Round 2 corrected
Length
739 aa
Mass
81.3 kDa
Annotated
2026-04-28
130 papers in source corpus 45 papers cited in narrative 45 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

VCAM-1 is a cytokine-inducible immunoglobulin superfamily transmembrane glycoprotein that serves as a central adhesion and signaling hub on endothelial, stromal, and other cell types, mediating leukocyte, hematopoietic stem cell, and cancer cell interactions through binding VLA-4 (α4β1) and α9β1 integrins (PMID:2688898, PMID:10209034). Its transcription is driven by NF-κB and GATA elements in the proximal promoter and is further regulated by Notch1/Jagged1 signaling, HDAC1/2–STAT3–GATA6 epigenetic circuits, miR-126, and FAK–GATA4 nuclear ubiquitination pathways (PMID:1379595, PMID:18227515, PMID:33859766, PMID:22734001, PMID:26646450). Engagement of VCAM-1 by integrin-bearing leukocytes activates endothelial NADPH oxidase–derived H₂O₂, PKCα oxidation, and Ezrin/moesin-dependent actin docking structures that facilitate transendothelial migration, while its extracellular domain is proteolytically shed by ADAM17 and neutrophil serine proteases to modulate adhesion in inflammation and hematopoietic progenitor mobilization (PMID:17056569, PMID:12082081, PMID:12878595, PMID:11520773). Beyond classical leukocyte trafficking, VCAM-1 regulates embryonic chorioallantoic fusion, neural stem cell quiescence via β-catenin signaling, HSC immune tolerance as a 'don't-eat-me' signal, brain endothelial aging-associated neuroinflammation, and cancer cell survival and metastasis through PI3K/Akt and AKT–mTOR pathways (PMID:7530222, PMID:28728023, PMID:35210567, PMID:31086348, PMID:22014578).

Mechanistic history

Synthesis pass · year-by-year structured walk · 20 steps
  1. 1989 High

    Identification of VCAM-1 as a new cytokine-inducible endothelial adhesion molecule for lymphocytes established it as a member of the immunoglobulin superfamily mediating selective leukocyte recruitment.

    Evidence Expression cloning by adhesion selection from IL-1/TNF-α–stimulated HUVEC cDNA library

    PMID:2688898

    Open questions at the time
    • Ligand on the leukocyte side not yet identified
    • In vivo function unknown
    • Signaling capacity of the cytoplasmic tail uncharacterized
  2. 1991 High

    Genomic characterization revealed alternative splicing producing 6- and 7-domain isoforms, and identification of NF-κB/GATA/AP1 promoter elements explained cytokine-inducible transcription, while the VLA-4 (α4β1) integrin was identified as the counter-receptor mediating B cell precursor retention in bone marrow.

    Evidence Genomic cloning, promoter-reporter analysis, EMSA, antibody-blocking adhesion assays with bone marrow fibroblasts and B cell precursors

    PMID:1707873 PMID:1715583 PMID:1715889

    Open questions at the time
    • Relative functional contribution of 6- vs 7-domain isoforms in vivo unclear
    • Other integrin counter-receptors not yet explored
    • Mechanism of NF-κB cell-type specificity not resolved
  3. 1992 High

    Detailed promoter dissection confirmed that tandem NF-κB sites and a GATA element are essential for TNF-α–induced transcription, ruling out mRNA stabilization as the primary induction mechanism.

    Evidence Deletion reporter constructs, nuclear run-on assays, EMSA in bovine aortic endothelial cells

    PMID:1281211 PMID:1379595

    Open questions at the time
    • Identity of the GATA family member driving endothelial expression unknown
    • Chromatin-level regulation not addressed
  4. 1995 High

    Three key mechanistic advances: (1) Vcam1 knockout mice revealed embryonic lethality from failed chorioallantoic fusion, establishing a non-immune developmental role; (2) domains 1 and 4 were shown to independently mediate VLA-4–dependent monocyte transmigration; (3) regulated metalloprotease-dependent ectodomain shedding was identified as a mechanism generating soluble VCAM-1.

    Evidence Targeted gene disruption in mice; domain-specific mAb blocking in transmigration assays; metalloprotease inhibitor studies with immunoprecipitation

    PMID:7529789 PMID:7530222 PMID:7545712

    Open questions at the time
    • Identity of the sheddase not yet determined
    • Whether placentation failure reflects adhesion vs signaling function unknown
    • Physiological role of soluble VCAM-1 undefined
  5. 1997 Medium

    Discovery that p38 MAPK selectively controls VCAM-1 surface expression post-transcriptionally (without affecting mRNA levels) revealed a layer of regulation beyond NF-κB–driven transcription.

    Evidence p38 inhibitor SB203580 in TNF-α–stimulated endothelial cells with parallel mRNA and surface protein quantification

    PMID:9020057

    Open questions at the time
    • Post-transcriptional mechanism (translation, trafficking, or stability) not specified
    • Only pharmacological inhibitor used, no genetic confirmation
  6. 1999 High

    Identification of α9β1 as a second integrin counter-receptor on neutrophils expanded VCAM-1's adhesion repertoire beyond VLA-4, explaining neutrophil interaction that was initially thought absent.

    Evidence Recombinant VCAM-1 adhesion assays and α9β1 antibody blocking in transendothelial migration

    PMID:10209034

    Open questions at the time
    • Relative contribution of α9β1 vs α4β1 in vivo not delineated
    • Structural basis of dual-integrin recognition unknown
  7. 2002 High

    Direct interaction of VCAM-1 with ezrin and moesin was demonstrated, and live imaging revealed VCAM-1/ICAM-1/ERM actin-rich docking structures that embrace adherent leukocytes, establishing a cytoskeletal signaling function for VCAM-1's cytoplasmic tail.

    Evidence GST pulldown for direct binding, live-cell dynamic fluorescence microscopy under flow, pharmacological inhibition of Rho/ROCK

    PMID:12082081

    Open questions at the time
    • Specific cytoplasmic tail residues mediating ERM binding not mapped
    • Whether docking structures are required for transmigration vs firm adhesion not resolved
  8. 2003 High

    ADAM17 (TACE) was identified as the specific metalloproteinase responsible for PMA-stimulated VCAM-1 ectodomain shedding, resolving the identity of the sheddase.

    Evidence ADAM17-deficient mouse-derived cells lack PMA-induced VCAM-1 shedding; metalloproteinase inhibitor pharmacology

    PMID:12878595

    Open questions at the time
    • Cleavage site not precisely mapped
    • Whether ADAM17 mediates constitutive shedding or only stimulated shedding unclear
  9. 2006 High

    Leukocyte binding to VCAM-1 was shown to activate endothelial NADPH oxidase, generating H₂O₂ that oxidizes and activates PKCα, which is required for transendothelial migration—linking VCAM-1 engagement to a reactive oxygen species signaling cascade.

    Evidence Dominant-negative PKCα, PKCα inhibitors, NADPH oxidase inhibition, anti-VCAM-1 cross-linking in endothelial cells under laminar flow

    PMID:17056569

    Open questions at the time
    • How VCAM-1 cytoplasmic tail couples to NADPH oxidase activation not defined
    • Whether PKCα oxidation is the sole downstream effector unknown
  10. 2008 High

    miR-126 was established as a post-transcriptional negative regulator of VCAM-1 in endothelial cells, with bidirectional gain- and loss-of-function demonstrating its role in tuning leukocyte adhesion.

    Evidence Transfection of miR-126 inhibitor/precursor in endothelial cells with VCAM-1 protein/mRNA quantification and leukocyte adhesion assay

    PMID:18227515

    Open questions at the time
    • Whether miR-126 directly targets VCAM-1 3ʹ UTR or acts indirectly not fully resolved
    • In vivo significance in inflammation not tested
  11. 2011 High

    VCAM-1 was repurposed in cancer: aberrant expression on breast cancer cells tethers macrophages via α4-integrins, activating Ezrin–PI3K/Akt pro-survival signaling in cancer cells; separately, VCAM-1 on bone micrometastases recruits osteoclast progenitors to switch dormancy to overt metastasis.

    Evidence Dominant-negative Ezrin, α4 blocking, TRAIL apoptosis and Akt assays, bone metastasis dormancy mouse models

    PMID:22014578 PMID:22137794

    Open questions at the time
    • Whether VCAM-1–Ezrin–Akt axis operates in non-breast cancers untested
    • Mechanism of VCAM-1 transcriptional reactivation in cancer cells not defined
  12. 2012 High

    Upstream regulatory complexity expanded: FAK–GATA4 nuclear pathway controls VCAM-1 via CHIP-mediated GATA4 ubiquitination, and IRF-1/miR-126 reciprocal regulation links lipid metabolism to VCAM-1 in atherogenesis.

    Evidence Conditional endothelial FAK KO, direct FAK–GATA4 binding, CHIP co-IP and ubiquitination assay; IRF-1/miR-126 bidirectional manipulation with monocyte arrest readout

    PMID:22734001 PMID:22874466

    Open questions at the time
    • Whether GATA4 or GATA6 predominates in different vascular beds unclear
    • FAK–GATA4 mechanism not tested outside cardiac endothelium
  13. 2015 Medium

    Notch1 intracellular domain was shown to directly induce VCAM-1 in the absence of inflammatory cytokines, adding a developmental signaling pathway to VCAM-1 regulation; concurrently, tumor-derived SPARC was found to induce endothelial VCAM-1 via p38 MAPK to promote cancer cell extravasation.

    Evidence Forced N1ICD expression, γ-secretase inhibition, Notch1/Jagged1 siRNA; SPARC manipulation with VCAM-1 blocking in melanoma lung metastasis model

    PMID:25925867 PMID:26646450

    Open questions at the time
    • Whether Notch1 binds the VCAM-1 promoter directly or acts through intermediaries not shown
    • Generalizability of SPARC–VCAM-1 axis across tumor types unknown
  14. 2017 High

    VCAM-1's intracellular signaling was shown to regulate β-catenin in embryonic radial glial cells, maintaining neural stem cell quiescence; separately, VCAM-1 synergized with DLL4 to enhance Notch signaling in HSPCs for T-lineage differentiation.

    Evidence Conditional Vcam1 KO in embryonic brain, β-catenin reporter, BrdU labeling; defined stromal-free DLL4/VCAM-1 co-presentation system with Notch reporter

    PMID:28394335 PMID:28728023

    Open questions at the time
    • How VCAM-1 cytoplasmic domain activates β-catenin mechanistically unknown
    • Whether VCAM-1 directly potentiates Notch receptor activation or acts through co-signaling unclear
  15. 2019 High

    Brain endothelial VCAM-1 was identified as a mediator of aging-associated neuroinflammation: aged plasma elevates brain endothelial VCAM-1, and genetic ablation or antibody blockade reverses microglial reactivity and cognitive decline in young mice receiving aged plasma.

    Evidence Conditional endothelial Vcam1 KO, anti-VCAM-1 antibody, parabiosis/plasma transfer, cognitive behavioral tests

    PMID:31086348

    Open questions at the time
    • Factor(s) in aged plasma responsible for VCAM-1 induction not identified
    • Whether VCAM-1 signals to microglia directly or recruits peripheral immune cells not resolved
  16. 2021 High

    Two new signaling cascades were delineated: (1) macrophage VLA-4/endothelial VCAM-1 engagement activates RAC1/ROS/PYK2/VE-cadherin phosphorylation to induce vascular permeability; (2) VCAM-1 with membrane-bound SCF on lipid bilayers synergistically promotes HSC adhesion, polarized morphology, and FOXO3a nuclear retention via PI3K.

    Evidence VLA-4/VCAM-1 overexpression/knockdown with vascular permeability assays and in vivo ascites model; reconstituted supported lipid bilayer with PI3K inhibitors and FOXO3a imaging

    PMID:33295887 PMID:34402812

    Open questions at the time
    • Whether PYK2 is a direct substrate of VCAM-1 outside-in signaling unknown
    • HSC bilayer findings not yet validated in bone marrow niche in vivo
  17. 2021 High

    An epigenetic circuit was uncovered: HDAC1/2 sustain VCAM-1 expression by preventing STAT3 acetylation-driven DNMT1 recruitment and GATA6 promoter methylation, linking chromatin remodeling to VCAM-1 transcriptional control in atherosclerosis.

    Evidence HDAC1/2 siRNA, romidepsin, STAT3 acetylation-blocking mutant, methylation-specific PCR, ChIP, ApoE⁻/⁻ mouse atherosclerosis model

    PMID:33859766

    Open questions at the time
    • Relative importance of GATA4 vs GATA6 in different endothelial beds unresolved
    • Whether HDAC3 has compensatory roles not tested
  18. 2022 High

    VCAM-1 on HSCs was revealed as a 'don't-eat-me' signal conferring innate immune tolerance, regulated by β2-microglobulin/MHC-I and recognized by PIR-B on phagocytes; additionally, VCAM-1–bearing endothelial extracellular vesicles were found to mobilize splenic neutrophils after myocardial infarction.

    Evidence Conditional Vcam1 KO in haplotype-mismatch transplantation, PIR-B KO phagocytes, phagocytosis assays; CRISPR/Cas9 VCAM-1 deletion in endothelial EV-producing cells with in vivo neutrophil mobilization

    PMID:35134856 PMID:35210567

    Open questions at the time
    • Whether VCAM-1 don't-eat-me function operates through direct PIR-B binding or via MHC-I co-presentation not distinguished
    • VCAM-1 EV cargo composition and receptor on splenic neutrophils undefined
  19. 2023 Medium

    A non-canonical role emerged: IL-33–induced microglial VCAM-1 senses plaque-associated ApoE to direct microglial chemotaxis toward amyloid-β plaques, establishing VCAM-1 as a chemotactic receptor in neurodegeneration.

    Evidence VCAM-1–ApoE interaction blocking, Aβ chemotaxis assays, Alzheimer's disease mouse model

    PMID:37735240

    Open questions at the time
    • VCAM-1–ApoE binding interface not structurally defined
    • Whether this represents a direct receptor–ligand interaction or requires co-receptors unknown
    • Relevance to human AD not established
  20. 2024 Medium

    Epigenetic and metabolic regulation of VCAM-1 was extended: H3K18 lactylation activates VCAM-1 transcription in gastric cancer, where VCAM-1 drives AKT–mTOR signaling and CXCL1-mediated immune cell recruitment; NF-κB–dependent tubular VCAM-1 was shown to mediate immune cell adhesion in kidney inflammation.

    Evidence H3K18 lactylation ChIP with VCAM-1 overexpression/knockdown and AKT–mTOR inhibitors; TNF-α/IL-1β treatment of tubular cells with NF-κB inhibition and Vcam1 genetic deletion

    PMID:38461905 PMID:39116349

    Open questions at the time
    • Whether lactylation-driven VCAM-1 induction is tumor-type specific unknown
    • Tubular VCAM-1 findings limited to in vitro models

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: how the VCAM-1 cytoplasmic tail couples to NADPH oxidase and β-catenin signaling; the structural basis for dual-integrin (α4β1/α9β1) and ApoE recognition; whether the 'don't-eat-me' function operates through direct PIR-B engagement; and the in vivo functional significance of the distinct 6- and 7-domain isoforms.
  • Cytoplasmic tail signaling adaptor identity unknown
  • No high-resolution structure of full-length VCAM-1 with integrin or ApoE
  • Isoform-specific functions not tested in vivo

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098631 cell adhesion mediator activity 8 GO:0048018 receptor ligand activity 3 GO:0060089 molecular transducer activity 3
Localization
GO:0005886 plasma membrane 7 GO:0005576 extracellular region 4 GO:0031410 cytoplasmic vesicle 1
Pathway
R-HSA-168256 Immune System 7 R-HSA-1500931 Cell-Cell communication 6 R-HSA-162582 Signal Transduction 6 R-HSA-1643685 Disease 5 R-HSA-1266738 Developmental Biology 4 R-HSA-1474165 Reproduction 1
Partners
ADAM17APOECTNNB1DLL4EZRITGA4ITGA9MSN

Evidence

Reading pass · 45 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1989 VCAM-1 was cloned as a novel cytokine-induced endothelial adhesion molecule that binds lymphocytes; it is a member of the immunoglobulin gene superfamily induced by IL-1 or TNF-alpha on human endothelial cells, mediating binding of mononuclear leukocytes but not neutrophils. Expression cloning by adhesion selection, transfection, mRNA induction assays Cell High 2688898
1991 The human VCAM1 gene contains 9 exons spanning ~25 kb; alternative splicing of exon 5 produces two VCAM-1 precursors (6-domain and 7-domain forms). The promoter contains functional NF-κB, GATA, and AP1 binding sites. Genomic cloning, Southern blotting, chromosomal mapping (human-mouse hybrid cell lines and in situ hybridization), mRNA splicing analysis Proceedings of the National Academy of Sciences of the United States of America High 1715583
1991 VCAM-1 binds B cell precursors to bone marrow fibroblasts via the integrin VLA-4 (CD49d/CD29); anti-VCAM-1 antibody blocked adhesion of normal B cell precursors, establishing the VLA-4/VCAM-1 axis in B cell precursor retention in bone marrow. Antibody blocking adhesion assays, flow cytometry, co-culture of B cell precursors with bone marrow fibroblasts The Journal of clinical investigation High 1715889
1991 A seven-domain (long) form of human VCAM-1 was cloned; both six-domain and seven-domain forms support adhesion of VLA-4-expressing cell lines, with the long form being the major form expressed on endothelium. cDNA cloning, immunoprecipitation with deglycosylation, transfection of COS7 cells, adhesion assay The Journal of biological chemistry High 1707873
1992 The VCAM-1 promoter is activated by TNF-alpha through two functional NF-κB sites at positions -77 and -63 bp; silencer elements between -1.641 kb and -288 bp restrict constitutive activity; NF-κB responsiveness is cell-type specific (present in endothelial cells but not Jurkat T cells). Deletion reporter (CAT) constructs transfected into bovine aortic endothelial cells, EMSA (gel retardation assays), TNF-alpha stimulation The Journal of biological chemistry High 1379595
1992 Functional NF-κB and GATA elements in the VCAM-1 core promoter are required for TNF-induced transcription in endothelial cells; new transcription (not mRNA stabilization) drives cytokine-induced VCAM-1 expression. Deletion reporter constructs, transfection into bovine aortic endothelial cells, nuclear run-on assays The Journal of experimental medicine High 1281211
1993 The murine VCAM1 gene has 10 exons spanning ~20 kb; exon 5 encodes a novel domain unique to murine VCAM-1 and inclusion by alternative splicing produces a truncated three-Ig-domain form anchored by a phosphatidylinositol linkage. The murine and human VCAM1 promoters share conserved NF-κB, Ets, and GATA binding sites. Genomic cloning, exon mapping, sequence analysis, alternative splicing characterization Genomics High 7507076
1995 Genetic ablation of murine Vcam1 causes failure of allantois-chorion fusion at E8.5, resulting in defective placentation and embryonic lethality, revealing an essential developmental role for VCAM-1 in chorioallantoic fusion. Targeted homologous recombination (knockout mice), embryological analysis, histology Genes & development High 7530222
1995 Both domain 1 and domain 4 of VCAM-1 independently support VLA-4 (α4β1)-dependent monocyte transendothelial migration; combined blockade of both domains is required for complete inhibition, and their relative contribution depends on the duration of endothelial IL-1 activation. Domain-specific anti-VCAM-1 mAb blocking assays, CHO cell transfection with 6-domain and 7-domain VCAM-1 constructs, monocyte migration assays under C5a gradient Journal of immunology High 7545712
1995 Membrane-bound VCAM-1 is converted to a soluble 100-kDa form by regulated metalloprotease-dependent proteolytic cleavage; EDTA and 1,10-phenanthroline (metalloprotease inhibitors) block this conversion, and phorbol ester induces shedding with concurrent loss of membrane-bound form. Biochemical characterization, metalloprotease inhibitor treatment, phorbol ester stimulation, immunoprecipitation of intracellular precursors and soluble forms in T cell clones Journal of immunology High 7529789
1997 TNF-alpha activates p38 MAP kinase in endothelial cells; p38 inhibition (SB203580) suppresses TNF-alpha-induced surface expression of VCAM-1 but not ICAM-1, without affecting VCAM-1 mRNA accumulation, indicating p38 regulates VCAM-1 at the post-transcriptional level. p38 MAPK inhibitor (SB203580), MAPKAP kinase 2 activity assays, Hsp27 phosphorylation, flow cytometry for surface expression, Northern blot for mRNA Biochemical and biophysical research communications Medium 9020057
1999 α9β1 integrin on neutrophils binds VCAM-1 and mediates stable adhesion and transendothelial neutrophil migration; α9β1 is highly expressed on neutrophils and contributes to chemotaxis across activated endothelium alongside α4 integrins. Cell adhesion assays with recombinant VCAM-1, antibody blocking of α9β1, transendothelial migration assays with TNF-alpha-activated HUVEC The Journal of cell biology High 10209034
1999 PPARα activators (fenofibrate, WY14643) inhibit TNF-alpha-induced VCAM-1 mRNA expression in human endothelial cells, in part by inhibiting NF-κB activation, and reduce monocyte adhesion; this identifies PPARα as a transcriptional regulator of VCAM-1. RT-PCR, transfection with VCAM-1 promoter deletion constructs, EMSA, monocyte adhesion assays, immunohistochemistry Circulation High 10377075
2000 VCAM-1 on fibroblast-like synoviocytes (FLS) interacts with CD49d/CD29 (VLA-4) on B cells to rescue synovial B cells from apoptosis and upregulate Bcl-xL expression in a contact-dependent manner; anti-VCAM-1 antibody blocked both effects. Co-culture of synovial fluid B cells with FLS, antibody blocking, viability assays (trypan blue, annexin V, Hoechst staining), Western blot for Bcl-xL Arthritis and rheumatism Medium 10817566
2000 RA synovial stromal cell VCAM-1 (CD106) interaction with CD49d/CD29 (VLA-4) on B cells induces Bcl-xL upregulation and protects B cells from apoptosis in a contact-dependent manner; anti-CD106 but not anti-CD54 antibody blocked these effects. Co-culture with stromal cell lines, antibody blocking, flow cytometry, Bcl-xL/Bcl-2 Western blot Journal of immunology Medium 10623863
2001 VCAM-1 expressed by bone marrow stromal cells is proteolytically cleaved by neutrophil serine proteases (neutrophil elastase and cathepsin G) during G-CSF-induced hematopoietic progenitor cell mobilization, enabling HSC egress. In vivo mouse mobilization model, immunohistochemistry, in vitro protease cleavage assays with recombinant VCAM-1 and purified neutrophil elastase/cathepsin G Blood High 11520773
2002 VCAM-1 interacts directly with moesin and ezrin (ERM proteins) and colocalizes with them at the apical endothelial surface. During leukocyte adhesion, VCAM-1, ICAM-1, activated moesin and ezrin cluster in an endothelial actin-rich docking structure that partially embraces the adherent leukocyte; this structure requires phosphoinositides and the Rho/ROCK pathway. In vitro direct binding assay (GST pulldown), live-cell imaging, immunofluorescence, siRNA-mediated knockdown, pharmacological inhibition of Rho/ROCK, dynamic fluorescence microscopy under flow The Journal of cell biology High 12082081
2003 PMA-stimulated shedding of VCAM-1 from the cell surface is mediated specifically by TACE (ADAM17), a metalloproteinase that cleaves VCAM-1 near the transmembrane domain to produce soluble VCAM-1; cells from ADAM17-deficient mice lack PMA-induced VCAM-1 shedding. Metalloproteinase inhibitors, ADAM17-deficient mouse-derived cells, VCAM-1 shedding assays, comparison with E-selectin/PECAM-1/ICAM-1 shedding The Journal of biological chemistry High 12878595
2006 Lymphocyte binding to VCAM-1 activates endothelial cell NADPH oxidase, generating ~1 μM H2O2, which in turn induces oxidation and activation of PKCα (phosphorylation at Thr638); this PKCα activation is required for VCAM-1-dependent leukocyte transendothelial migration. Dominant-negative PKCα overexpression, PKCα inhibitors (Rö-32-0432, Gö-6976), H2O2 scavenging, NADPH oxidase inhibition, anti-VCAM-1 antibody cross-linking, PKCα oxidation assay, spleen cell migration under laminar flow Journal of immunology High 17056569
2008 miR-126 is expressed by endothelial cells and inhibits VCAM-1 expression; reduction of miR-126 increases TNF-alpha-stimulated VCAM-1 expression and enhances leukocyte adherence, while overexpression of miR-126 precursor decreases VCAM-1 levels. Transfection of endothelial cells with miR-126 inhibitor or precursor oligonucleotides, VCAM-1 protein/mRNA quantification, leukocyte adhesion assay Proceedings of the National Academy of Sciences of the United States of America High 18227515
2011 VCAM-1 aberrantly expressed on breast cancer cells tethers macrophages via α4-integrins; clustering of cancer cell surface VCAM-1, acting through Ezrin, activates PI3K/Akt signaling and protects cancer cells from TRAIL-induced apoptosis in leukocyte-rich microenvironments such as the lung. Co-culture of breast cancer cells with macrophages, antibody blocking of α4-integrins, dominant-negative Ezrin, Akt activation assays, TRAIL apoptosis assay, in vivo lung metastasis mouse model Cancer cell High 22014578
2011 Aberrant VCAM-1 expression on breast cancer micrometastases in bone promotes transition from dormancy to overt metastasis by recruiting α4β1-positive monocytic osteoclast progenitors via VCAM-1/α4β1 interaction, elevating local osteoclast activity; anti-VCAM-1 and anti-α4 antibodies inhibit this process. Bone metastasis dormancy mouse model, NF-κB pathway manipulation, VCAM-1 overexpression, antibody blockade, osteoclast activity assays, in vivo imaging Cancer cell High 22137794
2012 IRF-1 and miR-126 reciprocally regulate VCAM-1 expression in response to triglyceride-rich lipoproteins (TGRL): proatherogenic TGRL increase IRF-1 and decrease miR-126, inducing VCAM-1; antiatherogenic TGRL do the opposite. Overexpression or silencing of IRF-1 or miR-126 recapitulates these effects. HAEC transfection with IRF-1 overexpression/silencing and miR-126 overexpression/silencing, VCAM-1 expression assay, monocyte arrest assay Circulation research Medium 22874466
2012 Nuclear-localized FAK (kinase-inhibited) binds GATA4 transcription factor via its FERM domain, promoting CHIP E3 ligase-dependent polyubiquitination and degradation of GATA4; loss of GATA4 reduces VCAM-1 expression. Active FAK facilitates TNF-α-induced MAPK activation and GATA4 stability, thereby supporting VCAM-1 expression. Pharmacological FAK inhibition, conditional endothelial FAK knockout mice, nuclear fractionation, direct FAK-GATA4 binding assay, CHIP co-immunoprecipitation, GATA4 ubiquitination assay, in vivo heart vessel analysis The Journal of cell biology High 22734001
2013 Metastatic tumor cell attachment induces endothelial VCAM-1 (dependent on tumor cell-clot/tissue factor formation); endothelial VCAM-1 recruits myeloid cells that support tumor cell survival and metastasis; VCAM-1 blocking antibody reduces myeloid recruitment and diminishes metastasis without affecting tumor cell adhesion. In vivo pulmonary metastasis mouse model, VCAM-1 blocking antibody, tissue factor pathway inhibitor/hirudin treatment, flow cytometry for myeloid cells, tumor cell survival assays Blood Medium 23407548
2014 VLA-4 (α4β1) on leukocytes interacts with VCAM-1 to mediate reciprocal NF-κB activation in both leukemia cells and bone marrow mesenchymal stromal cells; this bidirectional signaling promotes stromal-mediated chemoresistance, reversible by VLA-4/VCAM-1 blockade or NF-κB inhibition. Genome-wide gene expression profiling of BM-MSCs, co-culture, NF-κB reporter assays, mutant IκBα overexpression, VLA-4 blocking antibody, in vivo human leukemia BM microenvironment model Blood High 24599548
2015 Tumor-derived SPARC induces endothelial vascular permeability and cancer cell extravasation through VCAM-1 and p38 MAPK signaling; blocking VCAM-1 impedes SPARC-induced permeability and extravasation. Comparative proteomic analysis of melanoma secretomes, SPARC knockdown/overexpression, VCAM-1 blocking antibody, vascular permeability assays, lung metastasis mouse model Nature communications Medium 25925867
2015 VCAM-1 is a TGF-β1-inducible gene in lung fibroblasts (via transcriptional induction, not mRNA stabilization); VCAM-1 depletion inhibits fibroblast proliferation, causing G2/M and S phase arrest associated with reduced phospho-ERK1/2 and cyclin D1. TGF-β1 treatment, VCAM-1 siRNA depletion, mRNA stability assays, cell cycle analysis, Western blot for phospho-ERK1/2 and cyclin D1 Cellular signalling Medium 26386411
2016 oxLDL induces NF-κB activation and VCAM-1 expression through FAK-dependent activation of RSK (p90 ribosomal S6 kinase), which activates IKKβ; transgenic mice with endothelial-specific kinase-dead FAK show reduced RSK activity, decreased VCAM-1 expression, and reduced macrophage accumulation in atherosclerotic regions. RSK inhibitors, FAK kinase-dead transgenic mice, IKKβ activation assays, NF-κB reporter, monocyte adhesion assay, atherosclerosis model Journal of cell science High 26906414
2017 VCAM-1 expression in glioblastoma depends on EGFR expression, and is regulated via the p38/STAT3 signaling pathway; miR-181b, via protein phosphatase 2A-modulated SP-1 dephosphorylation, controls EGFR-dependent VCAM-1 expression and thereby monocyte adhesion to GBM cells through integrin α4β1. EGFR inhibition, VCAM-1 knockdown, integrin α4β1 blocking, miR-181b overexpression/inhibition, PP2A assay, SP-1 phosphorylation Western blot, 2D/3D co-culture adhesion assays Oncogene Medium 28459461
2017 VCAM-1 signals through its intracellular domain to regulate β-catenin signaling in embryonic radial glial cells (RGCs) in a context-dependent manner; loss of VCAM-1 in RGCs stimulates premature neuronal differentiation and prevents quiescence, depleting the embryonic origin of postnatal neural stem cells. Conditional Vcam1 knockout mice (region- and stage-specific), β-catenin reporter assays, BrdU labeling, clonal analysis, in vitro neural stem cell assays Neuron High 28728023
2017 VCAM-1 synergizes with Delta-like 4 (DLL4) to enhance Notch signaling in hematopoietic stem and progenitor cells and promote T-lineage differentiation; VCAM-1 additionally activates an inflammatory transcriptional program in nascent HSPCs. Defined stromal cell-free in vitro T cell differentiation system, DLL4/VCAM-1 protein presentation on surfaces, Notch reporter assays, flow cytometry for T cell progenitors, in vivo thymus colonization Nature methods Medium 28394335
2018 VCAM-1+ macrophage-like 'usher' cells patrol the inner surface of the venous plexus in zebrafish caudal haematopoietic tissue, interact with HSPCs via ITGA4, and direct HSPC retention at vascular homing hotspots. Advanced live imaging in zebrafish, cell-labelling system, ITGA4 blocking, in vivo loss-of-function Nature High 30455424
2019 Brain endothelial VCAM-1 mediates the detrimental effects of aged plasma on young brains: aged plasma elevates VCAM-1 on BECs; anti-VCAM-1 antibody administration or genetic ablation of Vcam1 in BECs counteracts microglial reactivity and cognitive deficits induced by aged plasma in young mice. Systemic anti-VCAM-1 antibody, conditional endothelial Vcam1 KO mice, parabiosis/plasma transfer model, microglial activation assays, cognitive behavioral tests Nature medicine High 31086348
2021 M2 macrophage VLA-4 (α4β1) engagement with endothelial VCAM-1 induces vascular permeability through a RAC1/ROS/PYK2 phosphorylation/VE-cadherin phosphorylation cascade; downregulation of VLA-4 or VCAM-1 reduces RAC1 and ROS, decreases p-PYK2 and p-VE-cadherin, and enhances barrier integrity. Targeting the VLA-4/VCAM-1 axis abrogates ascites formation in vivo. VLA-4 overexpression/knockdown, VCAM-1 overexpression in ECs, RAC1/ROS/PYK2/VE-cadherin signaling assays, vascular permeability assay, in vivo ovarian cancer ascites model The Journal of clinical investigation High 33295887
2021 HDAC1/2 promote endothelial VCAM-1 expression by suppressing STAT3 acetylation-dependent methylation (via DNMT1) of the GATA6 promoter; HDAC1/2 inhibition increases STAT3 acetylation, promotes STAT3-DNMT1 interaction, leads to GATA6 promoter hypermethylation, reduces GATA6 and VCAM-1 expression, and attenuates atherosclerosis. HDAC1/2 siRNA, Romidepsin treatment, methylation-specific PCR, ChIP for STAT3 at GATA6 promoter, STAT3 Lys685Arg acetylation-blocking mutant, DNMT1 co-immunoprecipitation, ApoE-/- mouse atherosclerosis model Theranostics High 33859766
2022 VCAM-1 on haematopoietic stem cells serves as a 'don't-eat-me' signal providing innate immune tolerance; this function is regulated by β2-microglobulin/MHC-I presentation on HSCs and paired Ig-like receptor-B (PIR-B) on phagocytes. VCAM-1 deletion in haplotype-mismatched settings impairs HSC engraftment due to phagocytic clearance. Vcam1 conditional KO, haplotype-mismatch transplantation, MHC-I/β2m blocking, PIR-B KO phagocytes, phagocytosis assays, in vivo HSC engraftment assays Nature cell biology High 35210567
2022 DLL4 and VCAM-1 synergize to activate Notch signaling in nascent HSPCs during endothelial-to-haematopoietic transition, enhancing T cell progenitor output ~80-fold; VCAM-1 additionally promotes an inflammatory transcriptional program in HSPCs. Defined serum/feeder-free PSC differentiation system, single-protein presentation, Notch reporter, transcriptomic analysis, flow cytometry, in vivo T cell maturation Science advances Medium 36001668
2023 IL-33 induces VCAM-1 in microglia, which promotes microglial chemotaxis toward amyloid-β plaques by sensing plaque-associated ApoE; disrupting the VCAM-1-ApoE interaction abolishes microglial Aβ chemotaxis and decreases Aβ clearance. IL-33 stimulation of microglia, VCAM-1 functional screening, VCAM-1-ApoE interaction blocking, Aβ chemotaxis assays, in vivo Alzheimer's disease mouse model Nature aging Medium 37735240
2021 Membrane-bound SCF and VCAM-1 synergistically regulate HSC morphology: HSCs cluster membrane-bound SCF at the HSC-bilayer interface and form a polarized morphology with large protrusion; VCAM-1 dramatically enhances HSC adhesion to the bilayer through a mechanism requiring PI3K signaling and cytoskeletal reorganization, and the synergy enhances nuclear retention of FOXO3a. Supported lipid bilayer reconstitution system, PI3K inhibitors, cytoskeletal disruption, FOXO3a localization by immunofluorescence, HSC adhesion force measurements The Journal of cell biology Medium 34402812
2015 Notch1 intracellular domain (N1ICD) in endothelial cells upregulates Jagged1 and directly induces VCAM-1 expression in the absence of inflammatory cytokines; Notch1/Jagged1 circuit amplifies IL-1β-induced VCAM-1 induction. γ-Secretase inhibition and Notch1/Jagged1 silencing abrogate IL-1β-mediated VCAM-1 induction. γ-Secretase inhibitor, Notch1/Jagged1 siRNA silencing, forced N1ICD expression, VCAM-1 quantification, in vivo chronic liver inflammation model Oncotarget Medium 26646450
2020 H. pylori infection increases VCAM-1 expression in cancer-associated fibroblasts via JAK/STAT1 signaling; CAF-derived VCAM-1 physically interacts with integrin αvβ1/5 on gastric cancer cells to facilitate tumor invasion in vitro and in vivo. RNA-seq of H. pylori-infected fibroblasts, JAK/STAT1 inhibitors, biolayer interferometry for VCAM1-integrin binding kinetics, zebrafish xenograft invasion model Oncogene Medium 32034307
2024 H3K18 lactylation in the gastric cancer tumor microenvironment transcriptionally activates VCAM-1; VCAM-1 in turn activates AKT-mTOR signaling to promote GC cell proliferation and migration, and upregulates CXCL1 via AKT-mTOR to recruit mesenchymal stem cells and M2 macrophages. H3K18 lactylation ChIP, VCAM-1 overexpression/knockdown, AKT-mTOR pathway inhibitors, CXCL1 quantification, macrophage/MSC recruitment assays, GC mouse models Biochemical pharmacology Medium 38461905
2024 Proinflammatory cytokines (TNF-α and IL-1β) induce NF-κB-dependent VCAM-1 expression in proximal tubule cells; tubular VCAM-1 overexpression or TNF-α stimulation increases splenocyte adhesion to tubular monolayers; pharmacological NF-κB inhibition or genetic Vcam1 deletion suppresses this tubule-immune cell adhesion. TNF-α/IL-1β treatment of immortalized mouse proximal tubular cells and primary renal cells, NF-κB inhibitors, MyD88/TRIF double-KO cells, VCAM-1 overexpression, splenocyte adhesion assay, single-cell transcriptomics of patient biopsies American journal of physiology. Renal physiology Medium 39116349
2022 VCAM-1-bearing endothelial cell-derived extracellular vesicles (EVs) are rapidly released after myocardial infarction and accumulate in the spleen, where they induce inflammatory gene expression and mobilize splenic neutrophils to peripheral blood; CRISPR/Cas9 deletion of VCAM-1 in endothelial cells removes the ability of EVs to provoke neutrophil mobilization. CRISPR/Cas9 VCAM-1-deficient endothelial cells, EV characterization (size/marker), in vivo splenic neutrophil mobilization assay, miRNA-126 in vivo inhibition, MI mouse model Cardiovascular research Medium 35134856

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2011 Genetic risk and a primary role for cell-mediated immune mechanisms in multiple sclerosis. Nature 2101 21833088
1989 Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes. Cell 1667 2688898
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2008 MicroRNA-126 regulates endothelial expression of vascular cell adhesion molecule 1. Proceedings of the National Academy of Sciences of the United States of America 857 18227515
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
1999 PPARalpha activators inhibit cytokine-induced vascular cell adhesion molecule-1 expression in human endothelial cells. Circulation 497 10377075
1994 Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene 492 8125298
2011 Macrophage binding to receptor VCAM-1 transmits survival signals in breast cancer cells that invade the lungs. Cancer cell 484 22014578
2002 Dynamic interaction of VCAM-1 and ICAM-1 with moesin and ezrin in a novel endothelial docking structure for adherent leukocytes. The Journal of cell biology 453 12082081
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2013 The intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes. The Journal of biological chemistry 413 23463506
2011 VCAM-1 promotes osteolytic expansion of indolent bone micrometastasis of breast cancer by engaging α4β1-positive osteoclast progenitors. Cancer cell 413 22137794
2005 Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome research 409 16344560
1992 Functional analysis of the human vascular cell adhesion molecule 1 promoter. The Journal of experimental medicine 404 1281211
1992 Characterization of the promoter for vascular cell adhesion molecule-1 (VCAM-1). The Journal of biological chemistry 388 1379595
2001 Vascular cell adhesion molecule-1 (CD106) is cleaved by neutrophil proteases in the bone marrow following hematopoietic progenitor cell mobilization by granulocyte colony-stimulating factor. Blood 373 11520773
2005 Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. Journal of proteome research 350 16335952
2019 Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nature medicine 339 31086348
1997 Selective expression of adhesion molecules on human brain microvascular endothelial cells. Journal of neuroimmunology 337 9184636
1998 Role of Rac1 and oxygen radicals in collagenase-1 expression induced by cell shape change. Science (New York, N.Y.) 320 9572733
1995 Targeted disruption of the murine VCAM1 gene: essential role of VCAM-1 in chorioallantoic fusion and placentation. Genes & development 309 7530222
2006 An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. The Journal of experimental medicine 289 17116732
2023 ICAM-1 and VCAM-1: Gatekeepers in various inflammatory and cardiovascular disorders. Clinica chimica acta; international journal of clinical chemistry 275 37442359
2014 Reciprocal leukemia-stroma VCAM-1/VLA-4-dependent activation of NF-κB mediates chemoresistance. Blood 245 24599548
2003 Stimulated shedding of vascular cell adhesion molecule 1 (VCAM-1) is mediated by tumor necrosis factor-alpha-converting enzyme (ADAM 17). The Journal of biological chemistry 234 12878595
1999 The integrin alpha9beta1 mediates adhesion to activated endothelial cells and transendothelial neutrophil migration through interaction with vascular cell adhesion molecule-1. The Journal of cell biology 230 10209034
1991 Vascular cell adhesion molecule-1 and the integrin VLA-4 mediate adhesion of human B cell precursors to cultured bone marrow adherent cells. The Journal of clinical investigation 227 1715889
2007 Genetic susceptibility to respiratory syncytial virus bronchiolitis is predominantly associated with innate immune genes. The Journal of infectious diseases 223 17703412
1993 Expression of VCAM-1 and E-selectin in an in vivo model of endothelial activation. The American journal of pathology 211 7689792
2009 Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. Science signaling 207 19738201
2000 beta-amyloid-induced migration of monocytes across human brain endothelial cells involves RAGE and PECAM-1. American journal of physiology. Cell physiology 193 11078691
1991 Gene structure, chromosomal location, and basis for alternative mRNA splicing of the human VCAM1 gene. Proceedings of the National Academy of Sciences of the United States of America 191 1715583
1997 p38 mitogen activated protein kinase regulates endothelial VCAM-1 expression at the post-transcriptional level. Biochemical and biophysical research communications 177 9020057
2015 Tumour-derived SPARC drives vascular permeability and extravasation through endothelial VCAM1 signalling to promote metastasis. Nature communications 160 25925867
2012 Nanobodies targeting mouse/human VCAM1 for the nuclear imaging of atherosclerotic lesions. Circulation research 154 22461363
2002 AGEs bind to mesothelial cells via RAGE and stimulate VCAM-1 expression. Kidney international 141 11786095
1991 Cloning of an alternate form of vascular cell adhesion molecule-1 (VCAM1). The Journal of biological chemistry 120 1707873
2018 VCAM-1+ macrophages guide the homing of HSPCs to a vascular niche. Nature 115 30455424
1996 Enhanced levels of soluble VCAM-1 in sickle cell patients and their specific increment during vasoocclusive crisis. Clinical immunology and immunopathology 101 8808648
2017 Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1. Nature methods 97 28394335
2012 IRF-1 and miRNA126 modulate VCAM-1 expression in response to a high-fat meal. Circulation research 88 22874466
2024 H3K18 lactylation-mediated VCAM1 expression promotes gastric cancer progression and metastasis via AKT-mTOR-CXCL1 axis. Biochemical pharmacology 87 38461905
2012 Nuclear-localized focal adhesion kinase regulates inflammatory VCAM-1 expression. The Journal of cell biology 85 22734001
2002 ICAM-1 and VCAM-1 mediate endotoxemic myocardial dysfunction independent of neutrophil accumulation. American journal of physiology. Regulatory, integrative and comparative physiology 85 12121861
2012 Adiponectin and leptin induce VCAM-1 expression in human and murine chondrocytes. PloS one 82 23285079
2014 Mobilization with granulocyte colony-stimulating factor blocks medullar erythropoiesis by depleting F4/80(+)VCAM1(+)CD169(+)ER-HR3(+)Ly6G(+) erythroid island macrophages in the mouse. Experimental hematology 75 24721610
2013 VCAM-1 and VAP-1 recruit myeloid cells that promote pulmonary metastasis in mice. Blood 71 23407548
2000 Rheumatoid arthritis synovial stromal cells inhibit apoptosis and up-regulate Bcl-xL expression by B cells in a CD49/CD29-CD106-dependent mechanism. Journal of immunology (Baltimore, Md. : 1950) 71 10623863
2006 Cytokines differentially regulate ICAM-1 and VCAM-1 expression on human gingival fibroblasts. Clinical and experimental immunology 70 16734619
2008 Changes in the expression of CD106, osteogenic genes, and transcription factors involved in the osteogenic differentiation of human bone marrow mesenchymal stem cells. Journal of bone and mineral metabolism 65 18600396
2015 VCAM-1 is a TGF-β1 inducible gene upregulated in idiopathic pulmonary fibrosis. Cellular signalling 64 26386411
2020 Cancer-associated fibroblasts-derived VCAM1 induced by H. pylori infection facilitates tumor invasion in gastric cancer. Oncogene 63 32034307
2017 MiR-181b modulates EGFR-dependent VCAM-1 expression and monocyte adhesion in glioblastoma. Oncogene 63 28459461
1995 Expression of VCAM-1 (CD106) by a subset of TCR gamma delta-bearing lymphocyte clones. Involvement of a metalloprotease in the specific hydrolytic release of the soluble isoform. Journal of immunology (Baltimore, Md. : 1950) 62 7529789
2016 Oxidized LDL induces FAK-dependent RSK signaling to drive NF-κB activation and VCAM-1 expression. Journal of cell science 60 26906414
2000 Regulation of synovial B cell survival in rheumatoid arthritis by vascular cell adhesion molecule 1 (CD106) expressed on fibroblast-like synoviocytes. Arthritis and rheumatism 60 10817566
2017 Follicular Stimulating Hormone Accelerates Atherogenesis by Increasing Endothelial VCAM-1 Expression. Theranostics 59 29187895
2006 VCAM-1 signals activate endothelial cell protein kinase Calpha via oxidation. Journal of immunology (Baltimore, Md. : 1950) 59 17056569
2021 Macrophage-mediated vascular permeability via VLA4/VCAM1 pathway dictates ascites development in ovarian cancer. The Journal of clinical investigation 58 33295887
1995 ICAM-1 and VCAM-1 in human renal allograft rejection. Kidney international 55 7543623
2017 Persistent Expression of VCAM1 in Radial Glial Cells Is Required for the Embryonic Origin of Postnatal Neural Stem Cells. Neuron 53 28728023
2014 SIRPA, VCAM1 and CD34 identify discrete lineages during early human cardiovascular development. Stem cell research 53 24968096
1996 Ibuprofen inhibits pyrogen-dependent expression of VCAM-1 and ICAM-1 on human endothelial cells. Life sciences 53 8649201
1999 Estimation of wound age and VCAM-1 in human skin. International journal of legal medicine 51 10335877
2023 Rapid neutrophil mobilization by VCAM-1+ endothelial cell-derived extracellular vesicles. Cardiovascular research 50 35134856
2021 Regulatory effects of IL-1β in the interaction of GBM and tumor-associated monocyte through VCAM-1 and ICAM-1. European journal of pharmacology 50 34058204
2018 Urinary angiostatin, CXCL4 and VCAM-1 as biomarkers of lupus nephritis. Arthritis research & therapy 49 29325582
2017 LINC00341 exerts an anti-inflammatory effect on endothelial cells by repressing VCAM1. Physiological genomics 48 28500253
2021 HDAC1 and 2 regulate endothelial VCAM-1 expression and atherogenesis by suppressing methylation of the GATA6 promoter. Theranostics 46 33859766
2020 VCAM-1 Target in Non-Invasive Imaging for the Detection of Atherosclerotic Plaques. Biology 46 33138124
2023 The VCAM1-ApoE pathway directs microglial chemotaxis and alleviates Alzheimer's disease pathology. Nature aging 43 37735240
2018 Purinergic receptor Y2 (P2Y2)- dependent VCAM-1 expression promotes immune cell infiltration in metabolic syndrome. Basic research in cardiology 43 30338362
2022 Mendelian randomization of genetically independent aging phenotypes identifies LPA and VCAM1 as biological targets for human aging. Nature aging 42 37118362
2010 P2Y2 receptor regulates VCAM-1 membrane and soluble forms and eosinophil accumulation during lung inflammation. Journal of immunology (Baltimore, Md. : 1950) 40 20720203
2009 Expression of ICAM1 and VCAM1 serum levels in rheumatoid arthritis clinical activity. Association with genetic polymorphisms. Disease markers 39 19597294
2008 Canonical Wnt pathway signaling suppresses VCAM-1 expression by marrow stromal and hematopoietic cells. Experimental hematology 39 18951693
2019 Human epithelial ovarian cancer cells expressing CD105, CD44 and CD106 surface markers exhibit increased invasive capacity and drug resistance. Oncology letters 38 31186752
2000 Budesonide and formoterol inhibit ICAM-1 and VCAM-1 expression of human lung fibroblasts. The European respiratory journal 38 10678623
1995 Domains 1 and 4 of vascular cell adhesion molecule-1 (CD106) both support very late activation antigen-4 (CD49d/CD29)-dependent monocyte transendothelial migration. Journal of immunology (Baltimore, Md. : 1950) 36 7545712
2014 Involvement of VCAM1 in the bovine conceptus adhesion to the uterine endometrium. Reproduction (Cambridge, England) 35 24803492
1995 A direct binding assay for the vascular cell adhesion molecule-1 (VCAM1) interaction with alpha 4 integrins. Cell adhesion and communication 35 8640376
1993 Regulation of VCAM-1 expression and involvement in cell adhesion to murine microvascular endothelium. Cellular immunology 35 7680963
2010 3,3'-Diindolylmethane decreases VCAM-1 expression and alleviates experimental colitis via a BRCA1-dependent antioxidant pathway. Free radical biology & medicine 34 21034812
2018 Endothelial NOD1 directs myeloid cell recruitment in atherosclerosis through VCAM-1. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33 30496704
2017 Heparan sulfate potentiates leukocyte adhesion on cardiac fibroblast by enhancing Vcam-1 and Icam-1 expression. Biochimica et biophysica acta. Molecular basis of disease 33 29222072
2022 DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells. Science advances 32 36001668
2020 Blocking VCAM-1 inhibits pancreatic tumour progression and cancer-associated thrombosis/thromboembolism. Gut 32 33087490
2017 VHL promotes immune response against renal cell carcinoma via NF-κB-dependent regulation of VCAM-1. The Journal of cell biology 32 28235946
2015 Clopidogrel Protects Endothelium by Hindering TNFα-Induced VCAM-1 Expression through CaMKKβ/AMPK/Nrf2 Pathway. Journal of diabetes research 32 26824050
2022 VCAM1 confers innate immune tolerance on haematopoietic and leukaemic stem cells. Nature cell biology 31 35210567
2022 Blocking VCAM-1 Prevents Angiotensin II-Induced Hypertension and Vascular Remodeling in Mice. Frontiers in pharmacology 31 35222039
2022 IMPDH inhibition activates TLR-VCAM1 pathway and suppresses the development of MLL-fusion leukemia. EMBO molecular medicine 31 36453131
1996 Expression of ICAM-1 and VCAM-1 in human malignant mesothelioma. The Journal of pathology 31 8774481
2015 PDE4 inhibition reduces neointima formation and inhibits VCAM-1 expression and histone methylation in an Epac-dependent manner. Journal of molecular and cellular cardiology 30 25640159
2003 Iron chelators inhibit VCAM-1 expression in human dermal microvascular endothelial cells. The Journal of investigative dermatology 28 12713595
2002 Rolling adhesion of human NK cells to porcine endothelial cells mainly relies on CD49d-CD106 interactions. Transplantation 28 11907429
1993 Structure of the murine VCAM1 gene. Genomics 28 7507076
2020 Human cardiac fibroblasts expressing VCAM1 improve heart function in postinfarct heart failure rat models by stimulating lymphangiogenesis. PloS one 27 32936824
2019 VCAM1 Is Induced in Ovarian Theca and Stromal Cells in a Mouse Model of Androgen Excess. Endocrinology 27 30951142
2000 Acute rejection before cytomegalovirus infection enhances von Willebrand factor and soluble VCAM-1 in blood. Kidney international 27 11115088
2001 Expression of PDGF-A, TGFb and VCAM-1 during the developmental stages of experimental atherosclerosis. European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes 26 11684832
2021 VCAM-1: closing the gap between lipotoxicity and endothelial dysfunction in nonalcoholic steatohepatitis. The Journal of clinical investigation 25 33720049
2016 Glycated LDL increase VCAM-1 expression and secretion in endothelial cells and promote monocyte adhesion through mechanisms involving endoplasmic reticulum stress. Molecular and cellular biochemistry 25 27206739
2010 Quantitative lymphatic vessel trait analysis suggests Vcam1 as candidate modifier gene of inflammatory bowel disease. Genes and immunity 25 20220769
2022 Ectopic Tumor VCAM-1 Expression in Cancer Metastasis and Therapy Resistance. Cells 24 36497180
2017 MicroRNA-1185 Promotes Arterial Stiffness though Modulating VCAM-1 and E-Selectin Expression. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 24 28441665
2013 Increased monocyte adhesion by endothelial expression of VCAM-1 missense variation in vitro. Atherosclerosis 24 24075742
2023 Human serum-derived exosomes modulate macrophage inflammation to promote VCAM1-mediated angiogenesis and bone regeneration. Journal of cellular and molecular medicine 23 36965158
2022 IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis. International journal of molecular sciences 23 35743247
2015 Activation of an endothelial Notch1-Jagged1 circuit induces VCAM1 expression, an effect amplified by interleukin-1β. Oncotarget 23 26646450
2005 Genomic rearrangements on VCAM1, SELE, APEG1and AIF1 loci in atherosclerosis. Journal of cellular and molecular medicine 22 15784173
2002 Soluble VCAM-1 is a very early marker of endothelial cell activation in cardiopulmonary bypass. Perfusion 21 11817523
2002 Signal transduction of beta2m-induced expression of VCAM-1 and COX-2 in synovial fibroblasts. Kidney international 21 11849381
2002 Phytochemical inhibition of interleukin-4-activated Stat6 and expression of VCAM-1. Biochemical and biophysical research communications 21 11944890
2021 Membrane-bound SCF and VCAM-1 synergistically regulate the morphology of hematopoietic stem cells. The Journal of cell biology 20 34402812
2015 Interaction Between Gastric Carcinoma Cells and Neural Cells Promotes Perineural Invasion by a Pathway Involving VCAM1. Digestive diseases and sciences 20 26108418
2010 MCP-1, ICAM-1 and VCAM-1 are present in early aneurysmal dilatation in experimental rats. Folia histochemica et cytobiologica 20 21071353
2003 Dexamethasone reduces lung eosinophilia, and VCAM-1 and ICAM-1 expression induced by Sephadex beads in rats. European journal of pharmacology 20 12729843
2023 Increase of ALCAM and VCAM-1 in the plasma predicts the Alzheimer's disease. Frontiers in immunology 19 36685605
2011 High-cell density-induced VCAM1 expression inhibits the migratory ability of mesenchymal stem cells. Cell biology international 19 21073443
2024 VCAM-1 mediates proximal tubule-immune cell cross talk in failed tubule recovery during AKI-to-CKD transition. American journal of physiology. Renal physiology 18 39116349
2023 Compound Kushen Injection inhibits epithelial-mesenchymal transition of gastric carcinoma by regulating VCAM1 induced by the TNF signaling pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology 18 37487253
2022 Activation of the Notch1-c-myc-VCAM1 signalling axis initiates liver progenitor cell-driven hepatocarcinogenesis and pulmonary metastasis. Oncogene 18 35256782
2020 IGFBP-3 stimulates human osteosarcoma cell migration by upregulating VCAM-1 expression. Life sciences 18 33188835
2016 Th1-Induced CD106 Expression Mediates Leukocytes Adhesion on Synovial Fibroblasts from Juvenile Idiopathic Arthritis Patients. PloS one 18 27123929
2015 Up-regulation of VCAM1 Relates to Neuronal Apoptosis After Intracerebral Hemorrhage in Adult Rats. Neurochemical research 18 25868755
2022 Loss of IRF7 accelerates acute myeloid leukemia progression and induces VCAM1-VLA-4 mediated intracerebral invasion. Oncogene 17 35256780
2002 Novel expression of vascular cell adhesion molecule-1 (CD106) by squamous epithelium in experimental acute graft-versus-host disease. The American journal of pathology 17 12213703