Affinage

UBE2J2

Ubiquitin-conjugating enzyme E2 J2 · UniProt Q8N2K1

Length
259 aa
Mass
28.9 kDa
Annotated
2026-06-10
41 papers in source corpus 20 papers cited in narrative 19 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

UBE2J2 is a tail-anchored, ER membrane-localized E2 ubiquitin-conjugating enzyme that serves as a central conjugating component of endoplasmic-reticulum-associated degradation (ERAD), conserved from its yeast ortholog Ubc6p, which ubiquitinates ERAD substrates on the cytosolic face of the ER as a prerequisite for their retrotranslocation and proteasomal degradation (PMID:9388185, PMID:12082160). It is anchored to the ER via a hydrophobic C-terminal tail with cytosolic-facing catalytic topology, is a functional E2 by in vitro thiol-ester assay, and is induced by the unfolded protein response (PMID:11278356, PMID:16720581). A defining feature of UBE2J2 is its capacity for noncanonical ubiquitination: it ubiquitinates serine/threonine residues through ester bonds even when lysines are available, a chemistry enabled by an active-site rearrangement that boosts E2-Ub thioester reactivity and a conserved histidine acting as a general base to activate the substrate hydroxyl, with RING E3 ligases sharpening serine selectivity allosterically (PMID:19951915, PMID:39533056). UBE2J2 functions as the cognate E2 for a broad set of ER-membrane E3 ligases—TMEM129, MARCH6/MARCHF6, CGRRF1, RNF145, and RNF139—to ubiquitinate substrates including MHC class I, FcRn, the Wnt cargo receptor EVI/WLS, and squalene monooxygenase, with substrate- and ligase-specific E2 usage that distinguishes it from UBE2G2 (PMID:24807418, PMID:25030448, PMID:29378775, PMID:30658189, PMID:31289263, PMID:41068091). It is exploited by viral E3 ligases (mK3, US11) to drive immune-evasion degradation of MHC-I and FcRn (PMID:19951915, PMID:24807418, PMID:25030448, PMID:31289263). Beyond catalysis, UBE2J2 acts as a membrane lipid-packing sensor whose transmembrane domain toggles between ubiquitin-loading-competent and inactive states with lipid order, coupling ER membrane homeostasis to ERAD output (PMID:41068091). UBE2J2 is itself an unstable, catalysis- and ER-localization-dependent substrate of proteasomal turnover (PMID:25083800), and is required for male meiotic progression in mice, where its loss arrests spermatocytes at mid-pachytene with defective crossover formation (PMID:40686610).

Mechanistic history

Synthesis pass · year-by-year structured walk · 17 steps
  1. 1997 High

    Established that an ER membrane-bound E2 (yeast Ubc6p, ortholog of UBE2J2) is a required upstream actor in ERAD, linking ubiquitination to retrograde extraction of misfolded substrates.

    Evidence Genetic deletion mutants and ERAD substrate degradation assays in yeast

    PMID:9388185

    Open questions at the time
    • Did not define the mammalian ortholog's role
    • Site of ubiquitin attachment and substrate range unresolved
  2. 2000 High

    Resolved where ubiquitination occurs by showing the Ubc6p/Ubc7p pair acts on cytosolic degrons on the ER's cytosolic face, independent of luminal transfer and Sec61/Hrd1.

    Evidence Genetic epistasis and degron mutagenesis in yeast

    PMID:10982838

    Open questions at the time
    • Substrate-recognition determinants in mammals untested
    • E3 ligase partners for these degrons not fully mapped
  3. 2001 High

    Defined UBE2J2 as a tail-anchored ER integral membrane protein in mammals and implicated ER-localized E2s in mammalian ERAD of TCR and CD3 subunits.

    Evidence Immunofluorescence, dominant-negative overexpression, pulse-chase in mammalian cells; yeast self-ubiquitination/turnover assays

    PMID:11278356 PMID:11406589

    Open questions at the time
    • Direct catalytic requirement of UBE2J2 (vs UBE2J1/Ubc7) for specific substrates not isolated
    • Cognate E3 ligases in mammals unidentified
  4. 2002 High

    Confirmed conserved cytoplasmic-face topology and ERAD function of mammalian Ubc6p-related E2s across species, generalizing the role to multiple substrates (TCR-alpha, mutant CFTR).

    Evidence Fractionation, topology assays, dominant-negative ERAD assays in mammalian cells and yeast

    PMID:12082160

    Open questions at the time
    • Endogenous loss-of-function effects not tested
    • Specific E2-E3 pairings undefined
  5. 2006 Medium

    Confirmed human UBE2J2 is a bona fide functional E2 with tail-anchor motifs and UPR-inducible expression, embedding it in the ER stress response.

    Evidence In vitro thiol-ester assay, localization, UPR induction in mammalian cells

    PMID:16720581

    Open questions at the time
    • No substrate or E3 partner defined in this study
    • Single-lab in vitro activity
  6. 2009 High

    Discovered that UBE2J2 catalyzes noncanonical ester-bond ubiquitination of serine/threonine even in the presence of lysines, redefining the chemistry of ubiquitin transfer it can perform.

    Evidence E2 pulldown, in vitro ubiquitination with lysine-less substrates, mass spectrometry of linkages (viral E3 mK3)

    PMID:19951915

    Open questions at the time
    • Structural basis of ester-bond catalysis not yet resolved
    • Generality across cellular E3 ligases untested
  7. 2014 High

    Identified UBE2J2 as the cognate E2 for the E3 TMEM129 in US11-driven MHC-I dislocation, establishing a specific E2-E3 axis in viral immune evasion.

    Evidence Genome-wide shRNA and CRISPR haploid screens, Co-IP, degradation assays

    PMID:24807418 PMID:25030448

    Open questions at the time
    • Whether the same axis operates on endogenous MHC-I turnover unclear
    • Role of ester vs lysine linkage on this substrate not dissected
  8. 2014 Medium

    Showed UBE2J2 is itself an unstable protein turned over by the proteasome in a manner dependent on its catalytic activity and ER localization, implying auto-regulatory turnover.

    Evidence Proteasome inhibitors, catalytic and truncation mutants, immunoblot

    PMID:25083800

    Open questions at the time
    • Direct auto-ubiquitination not demonstrated
    • Responsible E3, if any, not identified
  9. 2017 Medium

    Distinguished UBE2J2 from its paralog UBE2J1 by showing UBE2J2 is dispensable for ER stress recovery and is not the MAPK-phosphorylated/c-IAP1-interacting E2, sharpening functional non-redundancy.

    Evidence Phospho-mutant constructs, viability assays, Co-IP in mammalian cells

    PMID:28321712

    Open questions at the time
    • Whether UBE2J2 is regulated by any post-translational signal unknown
    • Negative result; single lab
  10. 2017 Medium

    Demonstrated E2 selectivity in viral ERAD, with UBE2J2 modulating TRC8/RNF139 levels while UBE2G2 drives US2-mediated HLA-I degradation, reinforcing E2-specific routing.

    Evidence Genome-scale CRISPR screen of all E2s with functional follow-up

    PMID:28743740

    Open questions at the time
    • Mechanism by which UBE2J2 downregulates TRC8 not defined
    • Single lab
  11. 2018 High

    Expanded the substrate/E3 repertoire by establishing UBE2J2 as the E2 for CGRRF1-mediated EVI/WLS degradation (Wnt secretion control) and for MARCH6-dependent SQLE degradation (cholesterol-regulated sterol metabolism), with substrate-specific E2 usage versus UBE2G2.

    Evidence RNAi/CRISPR screens, Co-IP, catalytic-mutant rescue, ERAD stability assays in multiple human cell types

    PMID:29378775 PMID:30658189

    Open questions at the time
    • Whether SQLE/EVI ubiquitination uses ester linkages unresolved
    • How E3 selection partitions substrates not mechanistically explained
  12. 2019 Medium

    Showed UBE2J2 within a Derlin-1/TMEM129 complex drives FcRn dislocation and degradation, providing a functional consequence (blockade of IgG transport) for its ERAD activity.

    Evidence Co-IP, degradation and IgG transcytosis assays in mammalian cells (US11 context)

    PMID:31289263

    Open questions at the time
    • Endogenous (non-viral) FcRn regulation not addressed
    • Single lab
  13. 2021 Medium

    Revealed UBE2J2 can build K11/K48/K63 linkages on EVI/WLS cooperatively with UBE2K and UBE2N independently of HRD1/GP78, with ERLIN2 bridging substrate to machinery, broadening its linkage and partner range.

    Evidence RNAi screen, Co-IP, ubiquitin linkage typing, E3-independence assays

    PMID:34406391

    Open questions at the time
    • Reconciliation with CGRRF1-dependent EVI degradation unclear
    • Direct E3 catalyzing these linkages not defined
  14. 2023 Medium

    Extended UBE2J2 function beyond the ER by showing it acts with UBE2K and the mitochondrial E3 MARCH5 to regulate Noxa/Bax-Mcl1 apoptotic signaling, modulating Venetoclax sensitivity in AML.

    Evidence Genome-wide CRISPR and epistasis screens, knockouts, apoptosis readouts in mouse AML cells

    PMID:36973350

    Open questions at the time
    • Mitochondrial vs ER pool of UBE2J2 not distinguished
    • Direct mitochondrial substrate of UBE2J2 not identified
  15. 2024 High

    Provided the structural and mechanistic basis for serine ubiquitination: active-site rearrangement enhances thioester reactivity and a conserved histidine acts as a general base, with RING E3s adding allosteric serine selectivity.

    Evidence X-ray crystallography, MD simulations, in vitro reconstitution and active-site mutagenesis

    PMID:39533056

    Open questions at the time
    • In-cell contribution of ester vs lysine linkages per substrate not quantified
    • Which physiological substrates are serine-ubiquitinated remains to be mapped
  16. 2025 High

    Demonstrated UBE2J2 is a membrane lipid-packing sensor whose transmembrane domain toggles ubiquitin-loading competence with lipid order, coupling ER membrane homeostasis to ERAD by multiple E3 ligases (RNF145, MARCHF6, RNF139).

    Evidence Reconstitution with purified ERAD factors and defined-lipid proteoliposomes, ubiquitination assays

    PMID:41068091

    Open questions at the time
    • In-cell evidence that physiological lipid changes toggle activity not shown
    • How lipid sensing integrates with E3 selection unresolved
  17. 2025 Medium

    Established a physiological requirement for UBE2J2 in male meiosis, with knockout mice azoospermic due to mid-pachytene arrest, unstable recombination intermediates, and failed crossover formation.

    Evidence Knockout mouse model, histology, HR-marker immunofluorescence, spermatocyte proteomics

    PMID:40686610

    Open questions at the time
    • Mechanistic link between UBE2J2 ubiquitination activity and recombination not defined
    • Whether ERAD substrates mediate the meiotic phenotype unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unknown which endogenous substrates are serine/ester-ubiquitinated in cells, how lipid sensing and E3 selection are integrated in vivo, and what molecular pathway connects UBE2J2 catalysis to meiotic recombination.
  • No in-cell quantification of ester vs lysine linkage per substrate
  • Mechanism coupling ERAD/ubiquitination to meiotic crossover formation undefined
  • Physiological lipid triggers of the sensor not identified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 4 GO:0016740 transferase activity 3 GO:0140299 molecular sensor activity 1
Localization
GO:0005783 endoplasmic reticulum 4 GO:0005739 mitochondrion 1
Pathway
R-HSA-392499 Metabolism of proteins 4 R-HSA-168256 Immune System 3 R-HSA-1430728 Metabolism 2 R-HSA-8953897 Cellular responses to stimuli 2 R-HSA-1474165 Reproduction 1 R-HSA-5357801 Programmed Cell Death 1
Partners
CGRRF1ERLIN2MARCH5MARCHF6RNF139RNF145TMEM129UBE2K
Complex memberships
Derlin-1/TMEM129/UBE2J2 ERAD dislocation complex

Evidence

Reading pass · 19 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 Ubc6p (yeast ortholog of UBE2J2) is a membrane-bound ER ubiquitin-conjugating enzyme that works together with Cue1p-assembled Ubc7p to ubiquitinate ERAD substrates; ubiquitination by this pair is a prerequisite for retrograde transport of lumenal substrates out of the ER. Genetic (deletion mutants), biochemical fractionation, ERAD substrate degradation assays in yeast Science High 9388185
2000 Ubc6p/Ubc7p ubiquitin-conjugating enzyme pair recognizes cytosolic degradation signals containing a patch of bulky hydrophobic residues and positively charged residues; ubiquitination of these substrates occurs on the cytosolic face of the ER without prior transfer to the ER lumen, independently of Hrd1p/Der3p and Sec61p retrograde transport. Genetic analysis with deletion mutants (hrd1/der3Δ, sec61-2), fusion protein degradation assays, mutational analysis of degron sequences in yeast Molecular and cellular biology High 10982838
2001 The tail-anchored ER membrane protein Ubc6p undergoes self-ubiquitination-dependent degradation that requires its own catalytic site cysteine and Cue1p-assembled Ubc7p, but is independent of Ubc1p, Hrd1p/Der3p, Hrd3p, Der1p, and the Sec61p translocon; a membrane-bound degradation intermediate accumulates in proteasome mutants. Genetic deletion mutants, sec61 mutants, pulse-chase degradation assays, proteasome mutant analysis in yeast The EMBO journal High 11406589
2001 Murine UBE2J2 (MmUbc6) is an integral membrane protein anchored via its hydrophobic C-terminal tail to the endoplasmic reticulum; overexpression of catalytically inactive MmUbc7 (not MmUbc6 dominant-negative) significantly delayed ERAD of T cell receptor alpha and CD3-delta subunits, implicating these ER-localized E2s in mammalian ERAD. Immunofluorescence colocalization, dominant-negative overexpression, pulse-chase degradation assays in mammalian cells The Journal of biological chemistry Medium 11278356
2002 Two families of mammalian Ubc6p-related proteins (including UBE2J2/NCUBE2) are localized to the ER membrane with the same cytoplasmic-face topology as yeast Ubc6p; expression of wild-type and dominant-negative alleles specifically affects ERAD of TCR-alpha and mutant CFTR, and elevated Ubc6p levels in yeast also affect ERAD, indicating highly conserved function. Subcellular fractionation, topology assays, dominant-negative overexpression, ERAD substrate degradation assays in mammalian cells and yeast Journal of cell science High 12082160
2006 Human UBE2J2 (hsUbc6) possesses tail-anchored protein motifs, is localized to the ER, is a functional ubiquitin-conjugating enzyme as shown by in vitro thiol-ester assay, and its expression is induced by the unfolded protein response. In vitro ubiquitin thiol-ester assay, subcellular localization, UPR induction experiments The Journal of biological chemistry Medium 16720581
2009 UBE2J2 is the primary cellular E2 recruited by the viral E3 ligase mK3 for ERAD; the UBE2J2-mK3 pair preferentially ubiquitinates hydroxylated amino acids (serine/threonine) on ERAD substrates via ester bonds even when lysine residues are present, establishing that noncanonical ubiquitination of hydroxylated residues is physiologically relevant. E2 identification by co-immunoprecipitation/pulldown, in vitro ubiquitination assay with lysine-less substrate mutants, mass spectrometry of ubiquitin linkages The Journal of cell biology High 19951915
2014 UBE2J2 is essential for US11-mediated ERAD of HLA class I molecules; TMEM129 (an E3 ligase) together with UBE2J2 (as its cognate E2) is responsible for ubiquitination, dislocation, and degradation of US11-associated MHC-I via a Derlin-1-dependent ERAD complex. Genome-wide shRNA screen, CRISPR forward genetic screen in near-haploid cells, Co-IP, functional degradation assays Nature communications / PNAS High 24807418 25030448
2014 UBE2J2 is itself an unstable protein subject to proteasomal degradation; proteasomal inhibitors increase its steady-state levels. This effect requires both catalytic activity and ER localization (disruption of either stabilizes the enzyme), suggesting auto-ubiquitination-linked turnover. Proteasome inhibitor treatment, catalytic mutants, truncation mutants, immunoblot in transfected mammalian cells Biochemical and biophysical research communications Medium 25083800
2017 UBE2J2, in contrast to UBE2J1, is not essential for recovery from transient ER stress; Ube2j1 (not Ube2j2) is the E2 whose phosphorylation by MAPK signaling mediates ER stress recovery, and c-IAP1 preferentially interacts with phosphorylated Ube2j1. Ectopic expression of wild-type and phospho-mutant constructs, cell viability assays, Co-IP in mammalian cells Journal of cell communication and signaling Medium 28321712
2017 UBE2J2 counteracts US2-induced ERAD by downregulating TRC8 (RNF139) E3 ligase expression; in contrast, UBE2G2 is the crucial E2 for US2-mediated HLA-I degradation, demonstrating distinct roles for different E2s in HCMV immune evasion. Lentiviral CRISPR/Cas9 library screening targeting all human E2 enzymes, functional degradation assays Journal of cell science Medium 28743740
2018 UBE2J2 functions as the E2 ubiquitin-conjugating enzyme for the E3 ligase CGRRF1 to ubiquitinate the Wnt cargo receptor Evi/Wls for ERAD in the absence of Wnt ligands, thereby regulating Wnt protein secretion. RNAi knockdown, co-immunoprecipitation, ERAD substrate stability assays in mammalian cells The EMBO journal Medium 29378775
2018 UBE2J2 is the primary E2 ubiquitin-conjugating enzyme essential for MARCH6-dependent cholesterol-stimulated degradation of squalene monooxygenase (SQLE), but not for sterol-dependent degradation of HMGCR (which requires UBE2G2), revealing substrate-specific E2 usage in ERAD. CRISPR/Cas9-based screen for ERAD E2 enzymes, catalytic mutant rescue, cholesterol-stimulated degradation assays in multiple human cell types Atherosclerosis High 30658189
2019 US11 recruits Derlin-1, TMEM129, and UBE2J2 to engage FcRn, initiating dislocation of FcRn from the ER to the cytosol and its degradation, thereby inhibiting IgG-FcRn binding and IgG transport. Co-immunoprecipitation, ERAD substrate degradation assays, functional IgG transcytosis assays in mammalian cells Nature communications Medium 31289263
2021 UBE2J2, together with UBE2K and UBE2N, mediates K11-, K48-, and K63-linked ubiquitylation of EVI/WLS, independently of E3 ligases HRD1 and GP78; ERLIN2 links EVI/WLS to the ubiquitylation machinery. RNAi screen, Co-IP, ubiquitin linkage analysis, E3 ligase independence assays in mammalian cells Journal of cell science Medium 34406391
2023 Ube2j2 functions cooperatively with Ube2k as E2 ubiquitin-conjugating enzymes with the E3 ligase March5 at the mitochondria; depletion of Ube2j2 sensitizes AML cells to Venetoclax specifically in the presence of March5, and March5 controls Noxa levels to regulate Bax-Mcl1 interactions in the apoptosis pathway. Genome-wide CRISPR/Cas9 screens, genetic knockouts, CRISPR epistasis screens in mouse AML cells Leukemia Medium 36973350
2024 X-ray crystallography and MD simulations of Ubc6/UBE2J2 revealed a two-layered mechanism for serine ubiquitination: (1) rearrangement of the active site enhances E2-Ub thioester reactivity toward weak nucleophiles; (2) a conserved histidine in Ubc6/UBE2J2 activates substrate serine via general base catalysis. RING-type E3 ligases further increase serine selectivity via an allosteric mechanism requiring specific positioning of the ubiquitin tail at the E2 active site. X-ray crystallography, molecular dynamics simulations, in vitro reconstitution ubiquitination assays, active-site mutagenesis The EMBO journal High 39533056
2025 UBE2J2 acts as a membrane lipid sensor in ERAD: in loosely packed membranes, its transmembrane domain associates with membrane lipids in a way that impedes ubiquitin loading (rendering it inactive), while tighter lipid packing promotes an active conformation and interaction with E1. This activity directs ubiquitin transfer by multiple E3 ligases (RNF145, MARCHF6, RNF139) targeting themselves and the substrate squalene monooxygenase. In vitro reconstitution with purified ERAD factors, lipid-composition modulation of proteoliposomes, ubiquitination assays Nature communications High 41068091
2025 UBE2J2 is essential for male meiosis in mice; Ube2j2 knockout male mice are azoospermic with spermatocytes arrested at mid-pachytene stage, exhibiting unstable homologous recombination intermediate complexes that fail to form crossovers, and loss of a broad set of meiosis- and chromosome segregation-associated proteins. Knockout mouse model, histology, immunofluorescence of HR markers (e.g., MLH1, RPA), proteomics of spermatocytes iScience Medium 40686610

Source papers

Stage 0 corpus · 41 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1997 Role of Cue1p in ubiquitination and degradation at the ER surface. Science (New York, N.Y.) 323 9388185
2001 Hsp70 molecular chaperone facilitates endoplasmic reticulum-associated protein degradation of cystic fibrosis transmembrane conductance regulator in yeast. Molecular biology of the cell 221 11359923
1997 Endoplasmic reticulum degradation: reverse protein flow of no return. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 210 9409541
2008 Degradation of a cytosolic protein requires endoplasmic reticulum-associated degradation machinery. The Journal of biological chemistry 118 18812321
2001 Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s). The Journal of biological chemistry 112 11278356
2001 Sec61p-independent degradation of the tail-anchored ER membrane protein Ubc6p. The EMBO journal 108 11406589
2014 A high-coverage shRNA screen identifies TMEM129 as an E3 ligase involved in ER-associated protein degradation. Nature communications 101 24807418
2002 A role for mammalian Ubc6 homologues in ER-associated protein degradation. Journal of cell science 97 12082160
2000 HRD gene dependence of endoplasmic reticulum-associated degradation. Molecular biology of the cell 95 10793145
2009 Ube2j2 ubiquitinates hydroxylated amino acids on ER-associated degradation substrates. The Journal of cell biology 91 19951915
2014 TMEM129 is a Derlin-1 associated ERAD E3 ligase essential for virus-induced degradation of MHC-I. Proceedings of the National Academy of Sciences of the United States of America 86 25030448
2000 Degradation signals recognized by the Ubc6p-Ubc7p ubiquitin-conjugating enzyme pair. Molecular and cellular biology 79 10982838
2003 Inositol 1,4,5-trisphosphate receptor ubiquitination is mediated by mammalian Ubc7, a component of the endoplasmic reticulum-associated degradation pathway, and is inhibited by chelation of intracellular Zn2+. The Journal of biological chemistry 46 12869571
2018 ERAD-dependent control of the Wnt secretory factor Evi. The EMBO journal 45 29378775
2002 Ubc6p and ubc7p are required for normal and substrate-induced endoplasmic reticulum-associated degradation of the human selenoprotein type 2 iodothyronine monodeiodinase. Molecular endocrinology (Baltimore, Md.) 45 12198238
2000 Ubiquitin-mediated proteolysis of a short-lived regulatory protein depends on its cellular localization. The Journal of biological chemistry 44 10991948
2001 Expression and degradation of the cystic fibrosis transmembrane conductance regulator in Saccharomyces cerevisiae. Archives of biochemistry and biophysics 37 11396922
2019 Human cytomegalovirus evades antibody-mediated immunity through endoplasmic reticulum-associated degradation of the FcRn receptor. Nature communications 35 31289263
2018 Differential use of E2 ubiquitin conjugating enzymes for regulated degradation of the rate-limiting enzymes HMGCR and SQLE in cholesterol biosynthesis. Atherosclerosis 32 30658189
2001 Ubiquitin-dependent 26S proteasomal pathway: a role in the degradation of native human liver CYP3A4 expressed in Saccharomyces cerevisiae? Archives of biochemistry and biophysics 30 11516167
2000 Identification of a family of noncanonical ubiquitin-conjugating enzymes structurally related to yeast UBC6. Biochemical and biophysical research communications 29 10708578
2017 The role of ubiquitin-conjugating enzyme Ube2j1 phosphorylation and its degradation by proteasome during endoplasmic stress recovery. Journal of cell communication and signaling 28 28321712
2006 Human homologs of Ubc6p ubiquitin-conjugating enzyme and phosphorylation of HsUbc6e in response to endoplasmic reticulum stress. The Journal of biological chemistry 26 16720581
1998 'ER degradation' of a mutant yeast plasma membrane protein by the ubiquitin-proteasome pathway. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26 9506475
2017 Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation. Journal of cell science 22 28743740
2010 Disulfide isomerase glucose-regulated protein 58 is required for the nuclear localization and degradation of retinoic acid receptor alpha. Reproduction (Cambridge, England) 22 20130111
2005 Vacuolar degradation of rat liver CYP2B1 in Saccharomyces cerevisiae: further validation of the yeast model and structural implications for the degradation of mammalian endoplasmic reticulum P450 proteins. Molecular pharmacology 20 15703377
2021 EVI/WLS function is regulated by ubiquitylation and is linked to ER-associated degradation by ERLIN2. Journal of cell science 19 34406391
2023 Targeting a mitochondrial E3 ubiquitin ligase complex to overcome AML cell-intrinsic Venetoclax resistance. Leukemia 18 36973350
2014 The human ubiquitin conjugating enzyme UBE2J2 (Ubc6) is a substrate for proteasomal degradation. Biochemical and biophysical research communications 12 25083800
2018 Ubc7/Ube2g2 ortholog in Entamoeba histolytica: connection with the plasma membrane and phagocytosis. Parasitology research 10 29594345
2017 UBE2J2 promotes hepatocellular carcinoma cell epithelial-mesenchymal transition and invasion in vitro. Oncotarget 10 29069742
1999 Cycloheximide, a new tool to dissect specific steps in ER-associated degradation of different substrates. Biological chemistry 9 10430031
2021 Integrating SNPs-based genetic risk factor with blood epigenomic response of differentially arsenic-exposed rural subjects reveals disease-associated signaling pathways. Environmental pollution (Barking, Essex : 1987) 8 34619179
2023 Identification and characterization of putative biomarkers and therapeutic axis in Glioblastoma multiforme microenvironment. Frontiers in cell and developmental biology 7 37538397
2024 Determinants of chemoselectivity in ubiquitination by the J2 family of ubiquitin-conjugating enzymes. The EMBO journal 5 39533056
2025 Structure predictions of MuRF1-UBE2 complexes identify amino acid residues governing interaction selectivity for each MuRF1-E2 pair. The FEBS journal 3 39930652
2024 A novel microRNA miR-4433a-3p as a potential diagnostic biomarker for lung adenocarcinoma. Heliyon 3 38765119
2025 UBE2J2 sensitizes the ERAD ubiquitination cascade to changes in membrane lipid saturation. Nature communications 2 41068091
2026 Proteomic Profiling Reveals Candidate Proteins and Pathways Associated with Chemo-Radio-Sensitivity and Relapse in Rhabdomyosarcoma. Journal of proteome research 0 41540731
2025 UBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in mice. iScience 0 40686610

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