| 2018 |
UBE2G1 plays a distinct role from UBE2D3 in substrate ubiquitination by CRL4CRBN: UBE2D3 acts to prime targets via monoubiquitination while UBE2G1 functions to extend polyubiquitin chains with lysine 48 linkages, as established by CRISPR-Cas9 genome-wide screening and functional validation. |
Genome-wide CRISPR-Cas9 screen with IKZF3 degron reporter, functional validation of E2 roles in CRL4CRBN-mediated substrate ubiquitination |
Blood |
High |
30042095 31300418
|
| 2011 |
UBE2G1 (and UBE2G2), members of the UBE2G family, cooperate with the CRL4(Cdt2) E3 ubiquitin ligase to polyubiquitylate and degrade the replication licensing factor Cdt1 after UV irradiation, an activity critical for preventing origin re-licensing in DNA-damaged cells. In contrast, UBE2G1 was not required for CRL4(Cdt2)-mediated ubiquitylation of p21 or Set8, nor for PCNA monoubiquitylation. |
siRNA knockdown, proteomic analysis of Cdt2-associated proteins, in vivo ubiquitylation assays, cell cycle analysis |
Molecular and cellular biology |
High |
21628527
|
| 2014 |
Ube2g1 synthesizes Lys-48-linked polyubiquitin chains without an E3 ligase; Tyr-102 and Tyr-104 in its acidic loop enhance ubiquitin binding and Lys-48-ubiquitylation activity, distinguishing Ube2g1 from the truncated form of Ube2r1. Intramolecular interaction between the acidic loop and the attached donor ubiquitin regulates this activity. |
In vitro ubiquitylation assays, NMR spectroscopy with oxyester and disulfide E2~UB thioester mimics, site-directed mutagenesis of acidic loop residues |
The Journal of biological chemistry |
High |
25471371
|
| 2019 |
A ubiquitin variant (UbV) targeting the backside of Ube2G1 (opposite the active site) impeded the generation of thioester-linked ubiquitin at the active-site cysteine of Ube2G1 by the E1 enzyme, indicating that the E2 backside is required for efficient E1-mediated charging of Ube2G1. |
Phage display selection of UbVs, crystal structure of UbV–Ube2G1 complex, in vitro charging assays |
Journal of molecular biology |
High |
31634471
|
| 2019 |
UBE2G1 has robust in vitro K48-linked chain extension activity with SCF cullin-RING ligases and buffers against the loss of UBE2R1/2 in human cells; UBE2G1 knockdown in cells lacking UBE2R1/2 results in stabilization of SCF substrates p27 and CYCLIN E, and the CUL2-RING ligase substrate HIF1α. |
Genome-wide CRISPR screen, in vitro ubiquitylation reconstitution with purified proteins, mass spectrometry-based quantification of E2 levels, siRNA knockdown with substrate stability readouts |
eLife |
High |
31868589
|
| 2021 |
CUL2LRR1 uses UBE2G1 (or UBE2G2) to extend a single K48-linked ubiquitin chain on CMG helicase MCM7 subunit after initial monoubiquitylation by UBE2D-class E2 enzymes, converting CMG into a substrate for p97 during DNA replication termination. |
In vitro reconstitution of CMG-MCM7 ubiquitylation with purified human proteins including neddylated CUL2LRR1 and a panel of E2 enzymes |
The Biochemical journal |
High |
34195792
|
| 2006 |
UBE2G1 cannot substitute for E2-25K (UBE2K) in supporting US11-triggered retro-translocation and polyubiquitination of MHC class I heavy chains in a permeabilized cell system, even though the related enzyme HsUbc7/Ube2G2 participates in ERAD. |
Permeabilized cell system with fractionated cytosol substitution, purified recombinant E2 complementation assay |
Proceedings of the National Academy of Sciences of the United States of America |
Medium |
16868077
|
| 2024 |
UBE2G1 forms a complex with the RING-type E3 ligase TRAF7 and mediates K48-linked polyubiquitination and proteasomal degradation of the circadian transcription factor DBP, thereby contributing to circadian period determination. |
Dominant-negative E2 screen (19 E2s), proteomic analysis of DBP-interacting proteins, co-immunoprecipitation, ubiquitination assay in cultured cells, TRAF7 overexpression/knockdown/knockout |
Communications biology |
Medium |
39379486
|
| 1996 |
UBE2G1 (originally named UBE2G) encodes a human ubiquitin-conjugating enzyme of 170 amino acids with 74% identity to C. elegans UBC7, strongly expressed in skeletal muscle, and maps to chromosome 17p13.3. |
cDNA cloning from fetal brain library, Northern blot, radiation-hybrid mapping |
Cytogenetics and cell genetics |
Medium |
8893823
|
| 2010 |
siRNA-mediated knockdown of Ube2g1 (together with Ube2i) in neonatal rat cardiomyocytes causes downregulation of α- and β-myosin heavy chain expression, disruption of sarcomere structures, and increased PRMT-I expression and ADMA synthesis, implicating Ube2g1 in maintaining cardiomyocyte sarcomere integrity via the ubiquitin-proteasome system. |
siRNA knockdown in primary neonatal rat cardiomyocytes, immunofluorescence for sarcomere structure, Western blot for myosin heavy chains and PRMT-I, ADMA measurement |
European heart journal |
Low |
20965886
|
| 2026 |
Elevated levels of Ube2g1 in young murine hematopoietic stem cells (HSCs) recapitulate hematopoietic aging hallmarks (increased myeloid-to-lymphoid ratio, reduced naïve T cells). Mechanistically, the effect was mediated through a Ube2g1–Shp2 axis that altered global tyrosine phosphorylation, rather than primarily through Ube2g1's ubiquitination function. |
Lentiviral overexpression of Ube2g1 in murine HSCs, bone marrow transplantation, flow cytometry for lineage output, phosphoproteomics/tyrosine phosphorylation analysis, Shp2 interaction studies |
Haematologica |
Medium |
41641641
|