Affinage

TRUB1

Pseudouridylate synthase TRUB1 · UniProt Q8WWH5

Length
349 aa
Mass
37.3 kDa
Annotated
2026-06-10
38 papers in source corpus 20 papers cited in narrative 20 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

TRUB1 is the human ortholog of bacterial TruB/yeast Pus4, a stand-alone pseudouridine synthase that installs the conserved Ψ55 modification in the T-loop of tRNAs and extends this activity to mRNA and microRNA biogenesis (PMID:12736709, PMID:28073919). In the nucleus it generates Ψ55 in most cytosolic elongator tRNAs, an activity that is partially restrained by a catalytically inactive nuclear PUS10 isoform that binds unmodified U54U55 tRNAs and blocks U55→Ψ55 conversion, while TRUB1 and PUS10 together act redundantly to ensure cytosolic tRNA Ψ55 (PMID:33023933, PMID:41136621). TRUB1 also pseudouridylates four mitochondrial tRNAs (tRNAAsn, tRNAGln, tRNAGlu, tRNAPro), and its loss disrupts tRNA base-pairing, conformation, and stability, impairing mitochondrial translation and oxidative phosphorylation biogenesis (PMID:36018806). Beyond tRNA, TRUB1 is the predominant mRNA pseudouridine synthase in mammalian cells, recognizing a defined sequence/structural context (PMID:28073919); single-site Ψ deposition stabilizes transcripts and enhances protein output, whereas clustered high-density Ψ reduces protein abundance, defining a density-dependent effect on translation (PMID:36302989, PMID:42011786). Independent of catalysis, TRUB1 directly binds the terminal loop of pri-let-7 through a conserved KRKK electrostatic interface and recruits the DGCR8 microprocessor to promote let-7 maturation and suppress cell proliferation (PMID:32926445, PMID:38776834). Conformational studies of the yeast ortholog further establish that TRUB1/Pus4 remodels tRNA structural dynamics in a manner separable from its enzymatic activity (PMID:41916762).

Mechanistic history

Synthesis pass · year-by-year structured walk · 19 steps
  1. 1997 High

    Established the enzymatic identity of the Ψ55 synthase, answering which protein forms the universally conserved T-loop pseudouridine in tRNA.

    Evidence Recombinant yeast Pus4 in vitro pseudouridylation of tRNA transcripts plus PUS4 gene disruption with chemical Ψ mapping

    PMID:9358157

    Open questions at the time
    • Did not address human ortholog activity
    • Substrate scope beyond tRNA untested
  2. 1998 Medium

    Showed the enzyme's substrate recognition is structure-based rather than strictly tRNA-sequence-dependent, by modifying a tRNA-like viral RNA domain.

    Evidence In vitro pseudouridylation of TYMV RNA variants with purified recombinant Pus4

    PMID:9705510

    Open questions at the time
    • Single lab, single study
    • In vivo relevance of viral RNA modification unknown
  3. 1999 Medium

    Delimited substrate specificity by showing the enzyme is not required for spliceosomal UsnRNA pseudouridylation.

    Evidence Chemical Ψ mapping in UsnRNAs from pus4Δ yeast

    PMID:10022901

    Open questions at the time
    • Negative result does not exclude other non-tRNA substrates
  4. 2000 High

    Revealed a catalysis-independent role in tRNA biogenesis, separating the protein's structural/scaffolding function from its enzymatic activity.

    Evidence High-copy PUS4 overexpression with GCN4-lacZ reporter, genetic epistasis, and activity-dead mutants in yeast

    PMID:10713174

    Open questions at the time
    • Molecular basis of tRNA processing/export interference not defined
    • Not tested for human TRUB1
  5. 2003 Low

    Identified the human ortholog TRUB1 and its conserved catalytic TruB domain, opening study of the mammalian enzyme.

    Evidence Gene cloning, phylogenetic/domain analysis, and Northern blot tissue survey

    PMID:12736709

    Open questions at the time
    • No direct enzymatic assay for human protein
    • Catalytic activity inferred from domain only
  6. 2006 Medium

    Placed the enzyme in a tRNA structural-stability pathway functionally overlapping with La protein.

    Evidence Genetic epistasis and tRNA stability assays in mutant tRNA(Arg) yeast strains

    PMID:16581807

    Open questions at the time
    • Mechanism of stabilization not resolved
    • Catalytic vs structural contribution unseparated here
  7. 2007 Medium

    Demonstrated a sequence-specific RNA-binding/antiviral activity distinct from pseudouridylation.

    Evidence Proteome array binding screen, in planta BMV accumulation, and in vitro virion reassembly assays

    PMID:17360619

    Open questions at the time
    • Physiological relevance to host cells unclear
    • Single lab
  8. 2014 High

    Established the enzyme as a bona fide mRNA pseudouridine synthase, extending its substrate repertoire beyond structured tRNA.

    Evidence PSI-seq, PUS4 deletion, and in vitro reconstitution showing necessity and sufficiency for TEF1 Ψ-239, conserved across species

    PMID:25353621

    Open questions at the time
    • Functional consequence of mRNA Ψ not addressed
    • Human ortholog mRNA activity not tested here
  9. 2017 High

    Identified TRUB1 as the dominant mammalian mRNA Ψ synthase and defined the sequence/structural code governing its specificity.

    Evidence Ψ-seq, TRUB1 knockdown/knockout, massively parallel reporter assays, and computational specificity modeling

    PMID:28073919

    Open questions at the time
    • Downstream functional impact of mRNA Ψ not yet defined
    • Mitochondrial substrates not addressed
  10. 2020 High

    Resolved compartmentalized division of labor for tRNA Ψ55, showing nuclear TRUB1 is regulated by an inactive nuclear PUS10 isoform.

    Evidence Recombinant assays, subcellular fractionation, specific synthase knockdown, and binding competition assays

    PMID:33023933

    Open questions at the time
    • Structural basis of PUS10 inhibition of TRUB1 unresolved
    • Regulation of compartment switching unknown
  11. 2020 High

    Uncovered a non-catalytic role in miRNA biogenesis: TRUB1 binds pri-let-7 and recruits DGCR8 to promote let-7 maturation and suppress proliferation.

    Evidence Luciferase reporter screen, HITS-CLIP, pulldown binding, let-7 maturation and proliferation assays

    PMID:32926445

    Open questions at the time
    • Whether other miRNAs are regulated unknown
    • Mechanism of DGCR8 recruitment not structurally defined here
  12. 2021 Medium

    Showed the yeast ortholog can adopt a heritable prion state altering proteostasis, growth, and lifespan.

    Evidence Prion formation, growth/size, lifespan, proteomic, and protein synthesis assays in [BIG+] yeast

    PMID:34545808

    Open questions at the time
    • No evidence human TRUB1 forms a prion
    • Link to canonical synthase activity unclear
  13. 2022 High

    Defined TRUB1 as the Ψ55 synthase for four specific mitochondrial tRNAs and linked its loss to defective mitochondrial translation.

    Evidence CRISPR knockout, CMC Ψ mapping, in vitro pseudouridylation, cDNA rescue, and mitochondrial translation assays

    PMID:36018806

    Open questions at the time
    • Why only four of 22 mt-tRNAs are substrates unresolved
    • OXPHOS phenotype mechanism not fully traced
  14. 2022 Medium

    Assigned a transcript-stabilizing function to TRUB1-installed mRNA Ψ in human cancer cells.

    Evidence BID-seq transcriptome-wide Ψ mapping with TRUB1 depletion and transcript stability assays

    PMID:36302989

    Open questions at the time
    • Stability mechanism (RNA-binding readers) not identified
    • Single lab
  15. 2024 Medium

    Provided the structural/molecular basis for pri-let-7 recognition, identifying a higher-eukaryote KRKK electrostatic interface.

    Evidence Biochemical binding, structural investigation, and KRKK motif mutagenesis on pri-let-7a1

    PMID:38776834

    Open questions at the time
    • Resolution/method of structure not specified
    • Single study
  16. 2025 High

    Established TRUB1/PUS10 redundancy for cytosolic tRNA Ψ55 and timing of Ψ deposition during pre-tRNA processing.

    Evidence Systematic PUS knockout/knockdown in HCT116 cells with BACS Ψ mapping

    PMID:41136621

    Open questions at the time
    • Functional consequence of redundancy buffering unclear
    • Regulation of processing-stage choice unknown
  17. 2025 High

    Separated catalytic from conformational-remodeling activities, showing the enzyme reshapes tRNA dynamics independent of Ψ formation.

    Evidence Single-molecule FRET and optical binding assays with catalytically inactive Pus4 mutant

    PMID:41916762

    Open questions at the time
    • Functional output of remodeling in vivo unknown
    • Demonstrated in yeast ortholog
  18. 2025 Low

    Linked TRUB1 loss to TNFα/NF-κB activation and BIRC3 regulation in colorectal cancer cells.

    Evidence TRUB1 knockdown in HCT116, RNA-seq, Western blot, and nude mouse tumor assays

    PMID:40260225

    Open questions at the time
    • TRUB1→BIRC3→NF-κB connection inferred from transcriptomics without direct mechanistic validation
    • Single lab
  19. 2026 High

    Established a density-dependent rule for how TRUB1-installed mRNA Ψ controls translation output.

    Evidence TRUB1 knockout with nanopore direct RNA-seq, proteomics, ribosome profiling, and in vitro translation

    PMID:42011786

    Open questions at the time
    • Reader proteins distinguishing single vs clustered Ψ not identified
    • Mechanism of translation impairment at high density unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How TRUB1 integrates its multiple activities — catalytic Ψ deposition, non-catalytic tRNA remodeling, and microprocessor recruitment — into coordinated cellular regulation, and what reader machinery interprets its mRNA marks, remains unresolved.
  • No Ψ reader proteins identified for TRUB1 marks
  • Coordination between catalytic and scaffolding roles unknown
  • Structural model of human TRUB1 on substrate tRNA/mRNA lacking

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140098 catalytic activity, acting on RNA 6 GO:0003723 RNA binding 3 GO:0016853 isomerase activity 2
Localization
GO:0005829 cytosol 2 GO:0005634 nucleus 1 GO:0005739 mitochondrion 1
Pathway
R-HSA-8953854 Metabolism of RNA 6 R-HSA-74160 Gene expression (Transcription) 1
Partners
DGCR8PUS10

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 Yeast Pus4 (encoded by YNL292w/PUS4) is the pseudouridine synthase responsible for forming Ψ55 in both cytoplasmic and mitochondrial tRNAs. Recombinant Pus4 purified from E. coli catalyzes Ψ55 formation on T7 in vitro transcripts of yeast tRNA genes with strict site-specificity (no other uridines modified), and deletion of YNL292w abolishes all Ψ55 formation activity in cell-free extracts. Recombinant protein purification, in vitro pseudouridylation assay on tRNA transcripts, gene disruption, chemical mapping of pseudouridine residues in cytoplasmic and mitochondrial tRNAs Nucleic acids research High 9358157
1998 Yeast Pus4 catalyzes Ψ55 formation in the tRNA-like 3′-domain of mutant TYMV RNA (at position 37 in TYMV-mutant G37U, equivalent to Ψ55 in tRNA), demonstrating substrate flexibility of Pus4 beyond canonical tRNA. In vitro pseudouridylation assay using purified recombinant yeast Pus4 on in vitro-transcribed TYMV RNA variants Nucleic acids research Medium 9705510
1999 Yeast PUS4 deletion does not affect pseudouridine formation in spliceosomal UsnRNAs (U1, U2, U4, U5, U6), establishing that Pus4 activity is not required for UsnRNA pseudouridylation and that its substrate specificity is restricted to tRNAs in this context. Chemical mapping of pseudouridine in UsnRNAs from pus4Δ yeast mutants Molecular and cellular biology Medium 10022901
2000 Overexpression of PUS4 in yeast causes accumulation of tRNA precursors and derepression of GCN4 translation (Gcd− phenotype) independently of eIF2α phosphorylation, by impeding tRNA 5′-end processing or nuclear export. Importantly, this Gcd− phenotype does not require PUS4 enzymatic activity, indicating a non-catalytic function of the protein in tRNA biogenesis. High-copy PUS4 overexpression, GCN4-lacZ reporter assay, genetic epistasis with RNase P (RPR1), LOS1, suppression analysis, enzymatic activity mutants Molecular and cellular biology High 10713174
2003 Human TRUB1 was identified as the first human ortholog of bacterial TruB/Ψ55 synthase, encoding a 349-amino acid protein with a conserved TruB domain (W104–I255) containing catalytic motif II with the conserved aspartate residue involved in uridine recognition and catalytic function. Northern blot showed wide tissue expression. Gene cloning, sequence/phylogenetic analysis, domain identification, Northern blot International journal of molecular medicine Low 12736709
2006 Yeast Pus4 is functionally redundant with La protein for tRNA structural stability: depletion of Pus4p in strains carrying a mutant tRNA(Arg)(CCG) decreases tRNA stability (while La deletion is lethal in this background), placing Pus4 in a pathway ensuring tRNA structural integrity and biogenesis. Genetic epistasis (double-mutant analysis), La deletion/Pus4 depletion in mutant tRNA strains, stability assays RNA (New York, N.Y.) Medium 16581807
2007 Yeast Pus4 (screened as an RNA-binding protein on proteome arrays) binds a CAM-containing RNA hairpin and inhibits BMV RNA encapsidation in plants and virion reassembly in vitro, demonstrating an RNA-binding antiviral activity beyond its pseudouridine synthase role. Proteome array binding screen, in planta BMV accumulation assay, in vitro virion reassembly assay Proceedings of the National Academy of Sciences of the United States of America Medium 17360619
2014 Yeast Pus4 modifies TEF1 mRNA at Ψ-239 in vivo, and this mRNA pseudouridylation is conserved in S. mikitae and S. pombe, establishing Pus4 as a mRNA pseudouridine synthase in addition to its tRNA role. Pus4 activity was shown to be necessary and sufficient for TEF1 Ψ-239 by genetic deletion and in vitro reconstitution. PSI-seq (transcriptome-wide pseudouridine mapping), genetic deletion of PUS4, in vitro reconstitution with purified Pus4 PloS one High 25353621
2017 Human TRUB1 is the predominant pseudouridine synthase acting on mammalian mRNA, targeting a specific sequence/structural context (computationally modeled with AUC=0.974 for substrate prediction). Genetic perturbation of TRUB1 combined with Ψ-seq and massively parallel reporter assays defined the sequence and structural determinants governing TRUB1 specificity. Ψ-seq on >2.5 billion reads, TRUB1 genetic knockdown/knockout, massively parallel reporter assays with thousands of synthetic sequence variants, computational modeling of specificity Genome research High 28073919
2020 Human TRUB1 (nuclear) produces Ψ55 in most elongator tRNAs in the nucleus, whereas cytoplasmic PUS10 produces Ψ55 (and Ψ54) in tRNAs that contain Ψ54Ψ55. The nuclear isoform of PUS10 (catalytically inactive) specifically binds unmodified U54U55 tRNAs and inhibits TRUB1-mediated U55→Ψ55 conversion, establishing compartmentalized and non-redundant Ψ55 synthase activities. Nearest-neighbor analysis, recombinant protein assays, subcellular fractionation, specific Ψ55 synthase knockdown cells, binding competition assays RNA (New York, N.Y.) High 33023933
2020 TruB1 directly binds the stem-loop structure of pri-let-7 miRNA (HITS-CLIP and biochemical assays showing binding to endogenous pri-let-7), selectively enhances interaction between pri-let-7 and the microprocessor DGCR8, and promotes let-7 maturation without pseudouridylating the miRNA. TruB1 suppresses cell proliferation partly via this let-7 pathway. Cell-based luciferase reporter screen, HITS-CLIP, biochemical binding assays (pulldown), let-7 maturation assays, cell proliferation assays The EMBO journal High 32926445
2021 The pseudouridine synthase Pus4/TruB can act as a prion in yeast ([BIG+] state), epigenetically increasing cell proliferation and size while shortening lifespan, with altered protein synthesis and differential synthesis of dozens of proteins including proliferation and aging regulators. Prion formation assays, cell growth/size measurements, lifespan assays, proteomic analysis of [BIG+] cells, protein synthesis measurements eLife Medium 34545808
2022 Human TRUB1 is responsible for Ψ55 formation in mitochondrial tRNAAsn, tRNAGln, tRNAGlu, and tRNAPro but not the other 18 mitochondrial tRNAs. TRUB1 knockout (CRISPR/Cas9) abolishes Ψ55 in these four tRNAs; recombinant TRUB1 efficiently catalyzes Ψ55 in tRNAAsn and tRNAGln in vitro. TRUB1 deficiency affects tRNA base-pairing (18A/G–Ψ55), conformation, and stability, and impairs mitochondrial translation and oxidative phosphorylation system biogenesis. CRISPR/Cas9 TRUB1 knockout, CMC/reverse transcription Ψ-mapping assay, in vitro pseudouridylation assay with recombinant TRUB1, cDNA rescue experiments, mitochondrial translation assay Nucleic acids research High 36018806
2022 BID-seq transcriptome-wide mapping in human cancer cells revealed that TRUB1-installed Ψ sites have a transcript stabilization role; depletion of TRUB1 reduced stability of transcripts bearing TRUB1-dependent Ψ modifications. BID-seq (bisulfite-induced deletion sequencing), TRUB1 knockdown/knockout, transcript stability assays Nature biotechnology Medium 36302989
2024 Human TruB1 binds specifically to the terminal loop of pri-let-7a1 at nucleotides 31–41 (a small stem-loop architecture). A conserved KRKK motif in human and higher eukaryotes provides an additional electrostatic binding interface beyond what is seen in E. coli TruB–tRNA interaction. The structural basis was determined by biochemical assays and structural investigation. Biochemical binding assays, structural investigation (crystal/structural study), mutagenesis of the KRKK motif Biochemical and biophysical research communications Medium 38776834
2025 TRUB1 and PUS10 function redundantly to catalyze the conserved Ψ55 modification in cytosolic tRNAs in human HCT116 cells; individual knockouts of TRUB1 or PUS10 each reduce but do not eliminate cytosolic tRNA Ψ55. Additionally, TRUB1 introduces Ψ modifications at distinct stages of pre-tRNA processing. Systematic PUS knockout/knockdown in HCT116 cells, BACS (2-bromoacrylamide-assisted cyclization sequencing) for Ψ mapping, comprehensive tRNA Ψ profiling Nature cell biology High 41136621
2025 Pus4/TruB (yeast ortholog of TRUB1) both catalyzes Ψ55 formation on tRNA and remodels tRNA conformational dynamics. Wild-type Pus4 binding to unmodified tRNA populates additional conformational states that gradually approach the ensemble adopted more rapidly by pre-pseudouridylated tRNA. A catalytically incompetent Pus4 mutant binds more slowly and remodels tRNA into distinct conformational ensembles, demonstrating that catalytic and remodeling activities are separable. Single-molecule FRET, optical binding assays, catalytically inactive Pus4 mutant analysis RNA (New York, N.Y.) High 41916762
2025 TRUB1 knockdown in CRC cells activates the TNFα/NFκB pathway, leading to increased expression of apoptosis-related proteins and decreased Ψ modification. BIRC3 was identified as a downstream target gene regulated by TRUB1 in the NFκB pathway. TRUB1 knockdown in HCT116 cells, RNA sequencing, Western blot, immunofluorescence, in vivo tumor growth assay in nude mice Gastroenterology report Low 40260225
2026 TRUB1-installed Ψ at single conserved sites on mRNAs is causally associated with increased protein production, as shown by TRUB1 knockout experiments demonstrating motif-specific reduction in protein abundance. In contrast, transcripts with clustered/high-density Ψ modifications show reduced protein abundance despite elevated translation efficiency, establishing a density-dependent effect of pseudouridylation on translation output. TRUB1 knockout, nanopore direct RNA sequencing (Mod-p ID), proteomics, ribosome profiling, controlled in vitro translation assays Nucleic acids research High 42011786
2025 Nanopore direct RNA sequencing of yeast mitochondria lacking PUS4 demonstrated that Pus4 pseudouridylates 23 of 24 mitochondrially-encoded tRNAs at Ψ55 in the T-loop in vivo. Nanopore direct RNA sequencing of mitochondrially-enriched RNA, PUS4 gene knockout comparison bioRxiv : the preprint server for biologypreprint Medium 40654949

Source papers

Stage 0 corpus · 38 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2014 Transcriptome-wide mapping of pseudouridines: pseudouridine synthases modify specific mRNAs in S. cerevisiae. PloS one 314 25353621
2021 Quantitative profiling of pseudouridylation dynamics in native RNAs with nanopore sequencing. Nature biotechnology 245 33986546
2022 Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution. Nature biotechnology 194 36302989
1997 The yeast gene YNL292w encodes a pseudouridine synthase (Pus4) catalyzing the formation of psi55 in both mitochondrial and cytoplasmic tRNAs. Nucleic acids research 144 9358157
1999 Pseudouridine mapping in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (snRNAs) reveals that pseudouridine synthase pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA. Molecular and cellular biology 129 10022901
2017 TRUB1 is the predominant pseudouridine synthase acting on mammalian mRNA via a predictable and conserved code. Genome research 119 28073919
2023 Quantitative profiling of pseudouridylation landscape in the human transcriptome. Nature chemical biology 115 36997645
2007 RNA-binding proteins that inhibit RNA virus infection. Proceedings of the National Academy of Sciences of the United States of America 86 17360619
2006 Formation of the conserved pseudouridine at position 55 in archaeal tRNA. Nucleic acids research 81 16920741
2000 Defects in tRNA processing and nuclear export induce GCN4 translation independently of phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2. Molecular and cellular biology 66 10713174
2015 PPUS: a web server to predict PUS-specific pseudouridine sites. Bioinformatics (Oxford, England) 64 26076723
2006 The La protein functions redundantly with tRNA modification enzymes to ensure tRNA structural stability. RNA (New York, N.Y.) 59 16581807
2008 Archaeal Pus10 proteins can produce both pseudouridine 54 and 55 in tRNA. RNA (New York, N.Y.) 48 18952823
2011 Pseudouridine formation in archaeal RNAs: The case of Haloferax volcanii. RNA (New York, N.Y.) 45 21628430
2024 Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptome. Nature methods 42 39349603
2022 Human TRUB1 is a highly conserved pseudouridine synthase responsible for the formation of Ψ55 in mitochondrial tRNAAsn, tRNAGln, tRNAGlu and tRNAPro. Nucleic acids research 37 36018806
2020 Mammalian nuclear TRUB1, mitochondrial TRUB2, and cytoplasmic PUS10 produce conserved pseudouridine 55 in different sets of tRNA. RNA (New York, N.Y.) 33 33023933
2020 The tRNA pseudouridine synthase TruB1 regulates the maturation of let-7 miRNA. The EMBO journal 31 32926445
2007 Differential roles of archaeal box H/ACA proteins in guide RNA-dependent and independent pseudouridine formation. RNA biology 30 17993784
2021 Mapping of pseudouridine residues on cellular and viral transcripts using a novel antibody-based technique. RNA (New York, N.Y.) 29 34376564
2003 The human TruB family of pseudouridine synthase genes, including the Dyskeratosis Congenita 1 gene and the novel member TRUB1. International journal of molecular medicine 27 12736709
1998 Pseudouridine and ribothymidine formation in the tRNA-like domain of turnip yellow mosaic virus RNA. Nucleic acids research 24 9705510
2021 A prion accelerates proliferation at the expense of lifespan. eLife 17 34545808
2024 mRNA psi profiling using nanopore DRS reveals cell type-specific pseudouridylation. bioRxiv : the preprint server for biology 7 38766185
2025 A comprehensive tRNA pseudouridine map uncovers targets dependent on human stand-alone pseudouridine synthases. Nature cell biology 4 41136621
2022 Intron-Dependent or Independent Pseudouridylation of Precursor tRNA Containing Atypical Introns in Cyanidioschyzon merolae. International journal of molecular sciences 4 36292915
2015 A susceptibility locus rs7099208 is associated with non-obstructive azoospermia via reduction in the expression of FAM160B1. Journal of biomedical research 3 26668583
2005 DEG1, encoding the tRNA:pseudouridine synthase Pus3p, impacts HOT1-stimulated recombination in Saccharomyces cerevisiae. Molecular genetics and genomics : MGG 3 16231152
2024 Structural basis of pri-let-7 recognition by human pseudouridine synthase TruB1. Biochemical and biophysical research communications 2 38776834
2025 Concurrent detection of chemically modified bases in yeast mitochondrial tRNAs by Nanopore direct RNA sequencing. bioRxiv : the preprint server for biology 1 40654949
2026 Trub1-mediated pseudouridylation is dispensable for immune cell development and homeostasis. Genes and immunity 0 41876669
2026 Chronology of tRNA structural dynamics prior to and during interaction with a pseudouridine synthase. RNA (New York, N.Y.) 0 41916762
2026 Multimodal profiling reveals cell type-specific pseudouridine modification and density-dependent translational regulation. Nucleic acids research 0 42011786
2025 TRUB1 is a novel biomarker for promoting malignancy in colorectal cancer via NFκB signaling. Gastroenterology report 0 40260225
2025 A pseudouridine synthase shapes tRNA structural dynamics through both catalysis and remodeling. Research square 0 40470188
2025 A pseudouridine synthase shapes tRNA structural dynamics through both catalysis and remodeling. bioRxiv : the preprint server for biology 0 40654928
2024 In silico characterization and identification of compound heterozygous variants in H/ACA Ribonucleoprotein Assembly Factor (SHQ1) from Indian population. Journal of family medicine and primary care 0 38482315
2024 Identifying key biomarkers and therapeutic candidates for post-COVID-19 depression through integrated omics and bioinformatics approaches. Translational neuroscience 0 39588145

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