Affinage

SSB

Lupus La protein · UniProt P05455

Length
408 aa
Mass
46.8 kDa
Annotated
2026-06-10
100 papers in source corpus 12 papers cited in narrative 12 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

The La/SSB protein is a nuclear RNA-binding protein that acts as a 3'-end chaperone for nascent RNA polymerase III transcripts, binding the UUU-3'OH termini of newly synthesized RNAs to shield them from exonucleolytic decay and to promote their maturation and assembly into functional ribonucleoprotein complexes (PMID:12045101). Recognition of the 3'-OH end is achieved by an unusual two-motif architecture in which the La motif engages UUU-3'OH using its edgewise and backside surfaces while leaving the canonical nucleic-acid-binding surfaces free, and RRM1's beta-sheet surface contacts the body of the bound RNA (PMID:20138158, PMID:16427005). This two-site engagement underlies substrate discrimination: La binds pre-tRNA more stably than processed tRNA or released 3'-trailer because precursors are held simultaneously through both the La motif and RRM1, and RRM1 loop-3 mutations selectively impair pre-tRNA/tRNA affinity, tRNA maturation, and an associated RNA chaperone activity without affecting UUU-3'OH binding alone (PMID:19287396). Beyond pre-tRNA processing, La participates in U snRNP biogenesis by stabilizing 3'-extended snRNA precursors to facilitate Sm protein loading and U4/U6 snRNP formation (PMID:10747032), and it promotes miRNA biogenesis by binding pre-miRNA stem-loops through all three RNA-binding motifs and protecting them from MCPIP1 nuclease-mediated decay (PMID:23129761). La also controls RNA fate at the level of localization, conferring nuclear retention of bound small RNAs (PMID:12045101, PMID:8608450), and possesses an ATP-dependent dsRNA unwinding activity that requires at least one 3'-overhang (PMID:9016572). Although predominantly nuclear, La/SSB relocalizes to the cell surface of apoptotic blebs, exposing it to extracellular autoantibodies (PMID:9794444).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1989 Medium

    Establishing the primary structure and domain organization of human La/SSB was the prerequisite for any mechanistic dissection of how it recognizes RNA.

    Evidence cDNA cloning and sequencing across mammalian species with recombinant fusion-protein epitope mapping

    PMID:2476998

    Open questions at the time
    • Did not define the RNA target sequence or the structural basis of binding
    • Autoepitope localization was descriptive and not linked to function
  2. 1996 Medium

    Addressed whether La binding alters RNA fate, showing that La occupancy dictates nuclear retention versus export of a bound small RNA.

    Evidence Xenopus oocyte injection of hY1 RNA mutants with RNA export and competition assays

    PMID:8608450

    Open questions at the time
    • Mechanism linking La binding to retention machinery not defined
    • Tested in a heterologous oocyte system for a single Ro RNP substrate
  3. 1997 Medium

    Tested whether La has enzymatic activity beyond passive binding, revealing an ATP-dependent dsRNA unwinding activity with defined substrate polarity.

    Evidence In vitro unwinding assays with native and recombinant La, antibody inhibition, and substrate-overhang specificity analysis

    PMID:9016572

    Open questions at the time
    • Physiological RNA substrates of the unwinding activity not identified
    • Single lab, not widely replicated
    • Relationship of helicase activity to chaperone and end-protection functions unresolved
  4. 2000 High

    Extended La function from Pol III transcripts to Pol II snRNAs, establishing that La stabilizes 3'-extended snRNA precursors to assist Sm protein loading and snRNP assembly.

    Evidence Yeast genetic epistasis (smd1-1 dependence on Lhp1p) with RNA binding and snRNP assembly assays in extracts

    PMID:10747032

    Open questions at the time
    • Demonstrated in yeast Lhp1p; conservation of the U4/U6 role in human cells not directly shown here
    • Quantitative contribution of La relative to other snRNP assembly factors unclear
  5. 2000 Medium

    Tested whether the La motif more broadly serves as an RNA-chaperone module by examining a La-motif protein integral to telomerase, supporting a generalizable RNA-assembly role.

    Evidence Co-purification, anti-p43 immunodepletion of telomerase activity, and in vitro RNA binding in Euplotes

    PMID:11080168

    Open questions at the time
    • Ortholog/analog study in Euplotes, not human La/SSB
    • Chaperone function inferred by analogy rather than directly assayed
  6. 2001 High

    Consolidated the core model that La protects UUU-3'OH ends of nascent Pol III transcripts to enable pre-tRNA maturation and RNP assembly.

    Evidence Synthesis of genetic and biochemical studies including mutant La analyses (review)

    PMID:12045101

    Open questions at the time
    • Did not resolve how precursors are distinguished from mature products at the molecular level
    • Breadth of transcripts dependent on La not exhaustively defined
  7. 2006 Medium

    Clarified the structural logic of end recognition, showing the La motif uses non-canonical surfaces for UUU-3'OH binding, freeing standard surfaces for additional RNA/DNA interactions.

    Evidence Crystal-structure interpretation of La-RNA complexes (perspective)

    PMID:16427005

    Open questions at the time
    • Proposed mRNA-related, chaperone-like, and DNA/chromatin activities not experimentally demonstrated here
    • Single structural interpretation
  8. 2009 High

    Resolved how La discriminates precursor from processed tRNA, showing two-site engagement (La motif + RRM1 beta-surface) gives stable pre-tRNA binding while single-site products are released rapidly.

    Evidence RRM1 loop-3 mutagenesis with in vitro binding and in vivo tRNA maturation assays

    PMID:19287396

    Open questions at the time
    • Structural basis of the RRM1-tRNA body contact not fully resolved
    • How handoff to downstream processing factors occurs unknown
  9. 2010 High

    Provided atomic-resolution confirmation of the two-motif binding mechanism through crystal structures of La-RNA complexes.

    Evidence Crystal structure determination of La-RNA complexes with biochemical validation

    PMID:20138158

    Open questions at the time
    • Structures captured specific complexes; dynamics of substrate release not directly visualized
    • Structural basis of helicase and chaperone activities not addressed
  10. 2012 Medium

    Expanded La's RNA repertoire to the miRNA pathway, showing it binds pre-miRNA stem-loops via all three motifs and protects them from MCPIP1 nuclease to promote miRNA biogenesis.

    Evidence Endogenous miRNA-precursor co-IP, in vitro binding with motif mutagenesis, and pre-miRNA stability assays against MCPIP1

    PMID:23129761

    Open questions at the time
    • Recognition of stem-loop rather than UUU-3'OH broadens but does not define a unified binding code
    • Single lab; quantitative impact on mature miRNA pools across the transcriptome not established
  11. 1998 Medium

    Documented that the predominantly nuclear La/SSB relocalizes to the surface of apoptotic blebs, linking its trafficking to autoantibody accessibility.

    Evidence Confocal immunofluorescence of apoptotic cardiocytes under permeabilized vs non-permeabilized conditions

    PMID:9794444

    Open questions at the time
    • Mechanism of nucleus-to-surface translocation unknown
    • Functional consequence beyond antigen exposure not defined
  12. 1999 Medium

    Placed La/SSB within antigen-processing endomembrane compartments, consistent with its presentation as an autoantigen.

    Evidence Subcellular fractionation with co-localization of MHC class II and cathepsins B/D in lacrimal acinar cells

    PMID:10564093

    Open questions at the time
    • Co-localization is correlative; direct presentation not shown
    • How a nuclear RNA-binding protein reaches these compartments is unexplained

Open questions

Synthesis pass · forward-looking unresolved questions
  • How La's distinct biochemical activities (end protection, two-site discrimination, ATP-dependent unwinding, pre-miRNA stabilization) are integrated and regulated, and what governs its translocation to the cell surface during apoptosis, remains unresolved.
  • No unified mechanism connecting helicase and chaperone activities to end-protection
  • Trigger and pathway for apoptotic surface exposure undefined
  • Physiological substrate range of the unwinding activity unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 6 GO:0140313 molecular sequestering activity 2 GO:0140098 catalytic activity, acting on RNA 1 GO:0140657 ATP-dependent activity 1
Localization
GO:0005634 nucleus 3 GO:0005886 plasma membrane 1
Pathway
R-HSA-8953854 Metabolism of RNA 4
Partners
MCPIP1

Evidence

Reading pass · 12 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 The La protein binds the 3' termini (UUU-3'OH) of newly synthesized RNA polymerase III transcripts, protecting these RNAs from exonucleases; this protection is required for normal pre-tRNA maturation and facilitates assembly of small RNAs into functional RNA-protein complexes, and contributes to nuclear retention of certain small RNAs. Genetic and biochemical analyses including mutant La protein studies Annual review of biochemistry High 12045101
2009 La protein preferentially binds pre-tRNAs over processed tRNAs or 3' trailer products through coupled use of two binding sites: one on the La motif (recognizing UUU-3'OH) and another on the RRM1 beta-surface that contacts elsewhere on the tRNA body. Two-site engagement provides stable pre-tRNA binding, while processed tRNA and 3' trailer are released from their single sites more rapidly. RRM1 loop-3 mutations decrease affinity for pre-tRNA and tRNA but not for the UUU-3'OH trailer alone, and impair tRNA maturation in vivo. RRM1 mutations also impair an RNA chaperone activity of La. Mutagenesis of RRM1 loop-3, in vitro binding assays, in vivo tRNA maturation assays Nature structural & molecular biology High 19287396
1997 The La/SSB autoantigen possesses an ATP-dependent double-stranded RNA unwinding (helicase) activity. Both native and recombinant La proteins from different species display this activity, which is inhibited by monospecific anti-La antibodies. La can unwind dsRNA substrates with at least one 3'-overhang; substrates with two 5'-overhangs are not unwound, indicating that at least one 3'-overhang is required. In vitro dsRNA unwinding assay with native and recombinant La proteins; inhibition by anti-La antibodies; substrate specificity analysis Nucleic acids research Medium 9016572
2010 The La protein contains two RNA-binding motifs (La motif/LAM and RRM1) arranged in a unique way. Crystal structures of La-RNA complexes reveal that the La motif recognizes UUU-3'OH using edgewise and backside surfaces of the motif, leaving the typical nucleic acid-binding surfaces available for other interactions. The RRM1 beta-sheet surface contacts other parts of the pre-tRNA. Crystal structure determination of La-RNA complexes combined with biochemical studies Biochimica et biophysica acta High 20138158
2000 The yeast La protein (Lhp1p) functions in the assembly of certain RNA polymerase II-transcribed U snRNAs into RNPs. Precursors to U1, U2, U4, and U5 RNAs are bound by Lhp1p. By stabilizing a 3'-extended form of U4 RNA, Lhp1p facilitates efficient Sm protein binding, thus assisting formation of the U4/U6 snRNP. Lhp1p becomes essential for accumulation of U4/U6 snRNPs at low temperatures in smd1-1 mutant cells. Genetic epistasis (smd1-1 mutant requiring Lhp1p), RNA binding assays, snRNP assembly assays in yeast extracts The EMBO journal High 10747032
2012 Human La/SSB binds precursor miRNA (pre-miRNA) stem-loop structures through all three RNA-binding motifs (LAM, RRM1, and RRM2), recognizing the characteristic stem-loop structure rather than requiring a 3' UUU terminus. La/SSB associates with endogenous pri-/pre-miRNAs and promotes miRNA biogenesis by stabilizing pre-miRNAs from MCPIP1 nuclease-mediated decay in mammalian cells. Co-immunoprecipitation of endogenous miRNA precursors, in vitro RNA binding assays, mutational analysis of RNA-binding motifs, pre-miRNA stability assays with MCPIP1 nuclease The Journal of biological chemistry Medium 23129761
1996 La protein binding to hY1 RNA (a Ro RNP component) causes nuclear retention of hY1 RNA in Xenopus oocytes, whereas an intact Ro60 binding site is required for nuclear export. Mutation of the La binding site on hY1 RNA results in nuclear export, while an intact La binding site causes strong nuclear retention. Xenopus laevis oocyte injection of hY1 RNA mutants, RNA export assays, competition studies RNA (New York, N.Y.) Medium 8608450
1998 In apoptotic human fetal cardiac myocytes, the La/SSB protein (normally predominantly nuclear in non-apoptotic cells) translocates to the cell periphery and is exposed on the surface of apoptotic blebs, where it becomes accessible to extracellular autoantibodies without cell permeabilization. Confocal microscopy and indirect immunofluorescence of apoptotic cardiocytes (staurosporine- and DMNQ-induced), permeabilized vs. non-permeabilized conditions Journal of immunology (Baltimore, Md. : 1950) Medium 9794444
1999 La/SSB protein co-localizes with MHC class II molecules and cathepsins B and D in endomembrane compartments of lacrimal acinar cells, including secretory vesicle membranes, prelysosomes, and trans-Golgi network microdomains, consistent with a role as an autoantigen processed and presented by these cells. Isopycnic centrifugation and phase partitioning to fractionate endomembrane compartments, followed by immunodetection of La/SSB, MHC class II, and cathepsins The American journal of physiology Medium 10564093
1989 Human La/SSB is a two-domain protein with an RNA-binding site located within the N-terminal domain. The complete protein sequence (408 residues) was determined from cDNA cloning. At least two distinct autoepitopes are located on separate structural domains. cDNA cloning and sequencing from multiple mammalian species, recombinant fusion protein epitope mapping Journal of autoimmunity Medium 2476998
2000 The Euplotes aediculatus telomerase component p43 contains an La motif and binds telomerase RNA in vitro. Anti-p43 antibodies immunodeplete both telomerase RNA and telomerase activity from nuclear extracts, indicating that p43 is a stoichiometric component of the active telomerase holoenzyme. By analogy to La proteins, p43 may function as an RNA chaperone for telomerase assembly. Co-purification of p43 with active telomerase, immunodepletion of telomerase activity with anti-p43 antibodies, in vitro RNA binding assay with recombinant p43 The EMBO journal Medium 11080168
2006 The La protein uses edgewise and backside surfaces of its RRM and winged-helix motifs for UUU-3'OH recognition, leaving the typical nucleic acid-binding surfaces available for other potential interactions such as mRNA-related and RNA chaperone-like activities and DNA/chromatin-associated activity. Crystal structure analysis of La-RNA complexes (review/perspective summarizing structural data) Molecular cell Medium 16427005

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2008 SSB as an organizer/mobilizer of genome maintenance complexes. Critical reviews in biochemistry and molecular biology 460 18937104
1999 Replication protein A (RPA): the eukaryotic SSB. Critical reviews in biochemistry and molecular biology 403 10473346
2001 The La protein. Annual review of biochemistry 357 12045101
1998 RecQ helicase, in concert with RecA and SSB proteins, initiates and disrupts DNA recombination. Genes & development 240 9553043
1991 Requirement for the replication protein SSB in human DNA excision repair. Nature 237 1992355
2009 SSB protein diffusion on single-stranded DNA stimulates RecA filament formation. Nature 220 19820696
1981 Sequences of the ssb gene and protein. Proceedings of the National Academy of Sciences of the United States of America 206 6270666
2005 Anti-Ro/SSA and La/SSB antibodies. Autoimmunity 178 15804706
2015 La FAM fatale: USP9X in development and disease. Cellular and molecular life sciences : CMLS 166 25672900
1995 Intra- and intermolecular spreading of autoimmunity involving the nuclear self-antigens La (SS-B) and Ro (SS-A). Proceedings of the National Academy of Sciences of the United States of America 163 7846070
2011 SSB functions as a sliding platform that migrates on DNA via reptation. Cell 160 21784244
2004 PriA helicase and SSB interact physically and functionally. Nucleic acids research 145 15576682
2017 Profiling Ssb-Nascent Chain Interactions Reveals Principles of Hsp70-Assisted Folding. Cell 141 28708998
2010 Conserved and divergent features of the structure and function of La and La-related proteins (LARPs). Biochimica et biophysica acta 140 20138158
1998 Detection of anti-Ro/SSA and anti-La/SSB autoantibody-producing cells in salivary glands from patients with Sjögren's syndrome. Arthritis and rheumatism 137 9870881
1999 MHC class II molecules, cathepsins, and La/SSB proteins in lacrimal acinar cell endomembranes. The American journal of physiology 129 10564093
2007 A central role for SSB in Escherichia coli RecQ DNA helicase function. The Journal of biological chemistry 127 17483090
2007 Dynamic structural rearrangements between DNA binding modes of E. coli SSB protein. Journal of molecular biology 126 17490681
2011 Structure of the SSB-DNA polymerase III interface and its role in DNA replication. The EMBO journal 123 21857649
1996 A hierarchy of SSB protomers in replication protein A. Genes & development 114 8804316
2006 RecJ exonuclease: substrates, products and interaction with SSB. Nucleic acids research 111 16488881
2007 High-resolution AFM imaging of single-stranded DNA-binding (SSB) protein--DNA complexes. Nucleic acids research 108 17392343
1998 Accessibility of SSA/Ro and SSB/La antigens to maternal autoantibodies in apoptotic human fetal cardiac myocytes. Journal of immunology (Baltimore, Md. : 1950) 102 9794444
2018 Dynamics of E. coli single stranded DNA binding (SSB) protein-DNA complexes. Seminars in cell & developmental biology 97 29588158
2008 RecG interacts directly with SSB: implications for stalled replication fork regression. Nucleic acids research 81 18986999
2007 SSB protein limits RecOR binding onto single-stranded DNA. The Journal of biological chemistry 81 17272275
2000 Euplotes telomerase contains an La motif protein produced by apparent translational frameshifting. The EMBO journal 80 11080168
2015 ATM prevents DSB formation by coordinating SSB repair and cell cycle progression. Proceedings of the National Academy of Sciences of the United States of America 74 25775545
2000 U snRNP assembly in yeast involves the La protein. The EMBO journal 74 10747032
1999 Fine specificity of the autoimmune response to the Ro/SSA and La/SSB ribonucleoproteins. Arthritis and rheumatism 71 10025913
1994 RNA- and single-stranded DNA-binding (SSB) proteins expressed during Drosophila melanogaster oogenesis: a homolog of bacterial and eukaryotic mitochondrial SSBs. Gene 71 8206370
2016 The tale of SSB. Progress in biophysics and molecular biology 64 27838363
2005 The SPRY domain of SSB-2 adopts a novel fold that presents conserved Par-4-binding residues. Nature structural & molecular biology 64 16369487
1996 The interactions with Ro60 and La differentially affect nuclear export of hY1 RNA. RNA (New York, N.Y.) 63 8608450
2009 Precursor-product discrimination by La protein during tRNA metabolism. Nature structural & molecular biology 60 19287396
1997 Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme. Nucleic acids research 58 9016572
2019 ¡vIVA la DivIVA! Journal of bacteriology 53 31405912
2015 Imaging and energetics of single SSB-ssDNA molecules reveal intramolecular condensation and insight into RecOR function. eLife 53 26381353
2013 Functional analysis of PGRP-LA in Drosophila immunity. PloS one 52 23922788
2012 Sjogren syndrome antigen B (SSB)/La promotes global microRNA expression by binding microRNA precursors through stem-loop recognition. The Journal of biological chemistry 52 23129761
2010 B-cell epitopes of the intracellular autoantigens Ro/SSA and La/SSB: tools to study the regulation of the autoimmune response. Journal of autoimmunity 51 20643529
2004 Anti-Ro/SSA and anti-La/SSB autoantibodies in the tear fluid of patients with Sjögren's syndrome. The British journal of ophthalmology 50 14977774
1997 Fine specificity of autoantibodies to La/SSB: epitope mapping, and characterization. Clinical and experimental immunology 50 9158085
2006 The La protein-RNA complex surfaces. Molecular cell 49 16427005
2016 Anti-RO/SSA and anti-La/SSB antibodies: Association with mild lupus manifestations in 645 childhood-onset systemic lupus erythematosus. Autoimmunity reviews 48 27988434
1983 Amplification of ssb-1 mutant single-stranded DNA-binding protein in Escherichia coli. Journal of molecular biology 46 6341603
2015 Remodeling of RecG Helicase at the DNA Replication Fork by SSB Protein. Scientific reports 42 25923319
2017 Glutamate promotes SSB protein-protein Interactions via intrinsically disordered regions. Journal of molecular biology 41 28782560
2006 Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein. Nucleic acids research 41 16707659
1991 Monomers of the Escherichia coli SSB-1 mutant protein bind single-stranded DNA. Journal of molecular biology 41 1988680
2017 The IDL of E. coli SSB links ssDNA and protein binding by mediating protein-protein interactions. Protein science : a publication of the Protein Society 40 28127816
2012 SSB-DNA binding monitored by fluorescence intensity and anisotropy. Methods in molecular biology (Clifton, N.J.) 39 22976177
2020 Distribution, organization an evolutionary history of La and LARPs in eukaryotes. RNA biology 38 32192383
2016 Toward faster and higher resolution LA-ICPMS imaging: on the co-evolution of LA cell design and ICPMS instrumentation. Analytical and bioanalytical chemistry 37 26753979
2007 Different temporal expression of immunodominant Ro60/60 kDa-SSA and La/SSB apotopes. Clinical and experimental immunology 37 17286801
1985 Immunologic and structural studies of the lupus/Sjögren's syndrome autoantigen, La/SSB, with a monoclonal antibody. The Journal of clinical investigation 37 2411763
1993 La proteins from Xenopus laevis. cDNA cloning and developmental expression. Journal of molecular biology 36 8510143
2021 Multiprotein E. coli SSB-ssDNA complex shows both stable binding and rapid dissociation due to interprotein interactions. Nucleic acids research 35 33434279
2019 Super-resolution imaging reveals changes in Escherichia coli SSB localization in response to DNA damage. Genes to cells : devoted to molecular & cellular mechanisms 35 31638317
2018 La/SSB chimeric autoantibody receptor modified NK92MI cells for targeted therapy of autoimmune disease. Clinical immunology (Orlando, Fla.) 35 29673902
2016 SSB binds to the RecG and PriA helicases in vivo in the absence of DNA. Genes to cells : devoted to molecular & cellular mechanisms 35 26766785
2019 The LARP1 La-Module recognizes both ends of TOP mRNAs. RNA biology 33 31601159
2022 Complement C4 Copy Number Variation is Linked to SSA/Ro and SSB/La Autoantibodies in Systemic Inflammatory Autoimmune Diseases. Arthritis & rheumatology (Hoboken, N.J.) 32 35315244
2019 Are the intrinsically disordered linkers involved in SSB binding to accessory proteins? Nucleic acids research 32 31329947
2010 A mechanism for single-stranded DNA-binding protein (SSB) displacement from single-stranded DNA upon SSB-RecO interaction. The Journal of biological chemistry 31 21169364
2019 Replicative DNA polymerases promote active displacement of SSB proteins during lagging strand synthesis. Nucleic acids research 29 30968132
1989 Sjögren's syndrome nuclear antigen B (La): cDNA cloning, structural domains, and autoepitopes. Journal of autoimmunity 29 2476998
2017 SSB and the RecG DNA helicase: an intimate association to rescue a stalled replication fork. Protein science : a publication of the Protein Society 28 28078722
2017 RecA-SSB Interaction Modulates RecA Nucleoprotein Filament Formation on SSB-Wrapped DNA. Scientific reports 28 28928411
2008 B cell apotopes of the 60-kDa Ro/SSA and La/SSB autoantigens. Journal of autoimmunity 28 18511235
2012 Pathogenicity and proteomic signatures of autoantibodies to Ro and La. Immunology and cell biology 27 22249199
1998 SSB protein controls RecBCD enzyme nuclease activity during unwinding: a new role for looped intermediates. Journal of molecular biology 27 9735287
2015 Binding dynamics of a monomeric SSB protein to DNA: a single-molecule multi-process approach. Nucleic acids research 26 26578575
2014 SSB binding to single-stranded DNA probed using solid-state nanopore sensors. The journal of physical chemistry. B 26 25222770
2013 Single molecule analysis of Thermus thermophilus SSB protein dynamics on single-stranded DNA. Nucleic acids research 26 24371279
2011 Novel, fluorescent, SSB protein chimeras with broad utility. Protein science : a publication of the Protein Society 26 21462278
2022 Replication stalling activates SSB for recruitment of DNA damage tolerance factors. Proceedings of the National Academy of Sciences of the United States of America 25 36191223
2012 Plasmodium falciparum SSB tetramer binds single-stranded DNA only in a fully wrapped mode. Journal of molecular biology 25 22543238
2020 Type I IFN system activation in newborns exposed to Ro/SSA and La/SSB autoantibodies in utero. RMD open 24 31958275
2005 Exposure and binding of selected immunodominant La/SSB epitopes on human apoptotic cells. Arthritis and rheumatism 24 16320341
2013 An immunodominant La/SSB autoantibody proteome derives from public clonotypes. Clinical and experimental immunology 23 23841690
1997 Identification and characterization of ssb and uup mutants with increased frequency of precise excision of transposon Tn10 derivatives: nucleotide sequence of uup in Escherichia coli. Journal of bacteriology 23 9139905
2018 SAAV2152 is a single-stranded DNA binding protein: the third SSB in Staphylococcus aureus. Oncotarget 22 29755648
2002 Candidate T cell epitopes of the human La/SSB autoantigen. Arthritis and rheumatism 22 11817592
2023 "Helicase" Activity promoted through dynamic interactions between a ssDNA translocase and a diffusing SSB protein. Proceedings of the National Academy of Sciences of the United States of America 21 37011199
2020 ¡Viva la mitochondria!: harnessing yeast mitochondria for chemical production. FEMS yeast research 21 32592388
2011 Terminating protein ubiquitination: Hasta la vista, ubiquitin. Cell cycle (Georgetown, Tex.) 21 21926471
2019 Regulation of Nearest-Neighbor Cooperative Binding of E. coli SSB Protein to DNA. Biophysical journal 19 31708161
2018 Crystal structure of SSB complexed with inhibitor myricetin. Biochemical and biophysical research communications 19 30213633
2017 Dynamic stepwise opening of integron attC DNA hairpins by SSB prevents toxicity and ensures functionality. Nucleic acids research 19 28985409
1994 The single-stranded-DNA-binding proteins (SSB) of Proteus mirabilis and Serratia marcescens. European journal of biochemistry 18 7925378
2022 The value of separate detection of anti-Ro52, anti-Ro60 and anti-SSB/La reactivities in relation to diagnosis and phenotypes in primary Sjögren's syndrome. Clinical and experimental rheumatology 17 36200940
2020 Structural dynamics in the La-module of La-related proteins. RNA biology 17 32186465
2018 Escherichia coli AlkB interacts with single-stranded DNA binding protein SSB by an intrinsically disordered region of SSB. Molecular biology reports 17 29974396
2017 The Hsp70 homolog Ssb affects ribosome biogenesis via the TORC1-Sch9 signaling pathway. Nature communications 17 29038496
2014 C-terminal domain swapping of SSB changes the size of the ssDNA binding site. BioMed research international 17 25162017
2012 Two distinct SSB protein families in nucleo-cytoplasmic large DNA viruses. Bioinformatics (Oxford, England) 16 23097418
2009 RNA recognition motif (RRM) of La/SSB: the bridge for interparticle spreading of autoimmune response to U1-RNP. Molecular medicine (Cambridge, Mass.) 16 19838329
1994 How does autoimmunity to La and Ro initiate and spread? Autoimmunity 16 7742478
1992 Anti-La antibodies. Rheumatic diseases clinics of North America 16 1626074

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