Affinage

TRIM27

Zinc finger protein RFP · UniProt P14373

Length
513 aa
Mass
58.5 kDa
Annotated
2026-04-28
100 papers in source corpus 30 papers cited in narrative 30 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

TRIM27 (RFP) is a RING-domain E3 ubiquitin ligase that ubiquitinates a broad spectrum of substrates with distinct chain linkages to regulate innate immunity, autophagy, endosomal trafficking, Polycomb-mediated chromatin regulation, and cell signaling. It catalyzes K48-linked ubiquitination of TBK1, NOD2, ULK1, p53, p21, PPARγ, and SIX3 for proteasomal degradation, attenuating type I interferon responses, NF-κB signaling, autophagy, and senescence (PMID:26358190, PMID:22829933, PMID:35670107, PMID:33865220, PMID:33251042); it also uses non-degradative ubiquitin chains (K6/K11 on STK38L, K33 on KLF12, atypical chains on PTEN) to modulate kinase activity, transcriptional repression, and phosphatase function (PMID:35670107, PMID:38924996, PMID:23419514). Beyond its ligase role, TRIM27 serves as an endosomal scaffold at retromer-positive structures—recruiting gp130/JAK1/STAT3 for IL-6 signaling and regulating WASH-dependent actin dynamics under the control of CLEC16A and WNK1—and is itself stabilized by the deubiquitinase USP7 within the non-canonical PRC1.1 Polycomb complex, where USP7 counteracts TRIM27 autoubiquitination to maintain chromatin occupancy and H2AK119ub levels (PMID:30143645, PMID:37307465, PMID:36538041, PMID:34113809). TRIM27 also functions as a ligase-independent transcriptional co-activator of TFEB via CREB1, driving autophagy gene expression to clear intracellular Mycobacterium tuberculosis (PMID:38390831).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1992 Medium

    Establishing that TRIM27 is a nuclear matrix-associated protein with DNA-binding activity provided the initial framework for understanding it as a nuclear factor rather than a cytoplasmic enzyme.

    Evidence Nuclear matrix fractionation and in vitro DNA-binding assay in cultured cells

    PMID:1437549

    Open questions at the time
    • No target gene regulation demonstrated
    • In vitro DNA binding not linked to specific genomic loci
    • Functional significance of nuclear matrix association unclear
  2. 1996 High

    Demonstrating that the RING finger is essential for RFP/RET oncogenic transformation, while the B box is dispensable, identified the RING domain as the critical functional module—presaging its later recognition as an E3 ligase domain.

    Evidence Site-directed mutagenesis of RING Cys/His residues with NIH 3T3 transformation and tyrosine phosphorylation readout

    PMID:8753810

    Open questions at the time
    • E3 ligase activity not yet recognized
    • Substrates of the RING domain unknown
    • RET-independent function of the RING not tested
  3. 1997 High

    Mapping homomultimerization to the coiled-coil domain and showing that RING/B-box mutations alter subcellular localization established the structural basis for TRIM27 oligomerization and compartmentalization.

    Evidence Yeast two-hybrid, mutagenesis, and immunofluorescence

    PMID:9247190

    Open questions at the time
    • Stoichiometry and higher-order assembly not resolved
    • Functional consequence of mislocalization not tested
  4. 2002 High

    Discovery that TRIM27 interacts with Enhancer of Polycomb 1 (EPC1) in an O-glycosylation-dependent manner and acts as a transcriptional repressor linked TRIM27 to chromatin regulation for the first time.

    Evidence GST pull-down, Co-IP from mouse testis, O-glycosidase treatment, and luciferase reporter assay

    PMID:11779184

    Open questions at the time
    • Genomic targets of repression unknown
    • Polycomb complex membership not yet defined
    • Physiological relevance in spermatogenesis not tested
  5. 2012 High

    Identification of TRIM27 as a bona fide E3 ubiquitin ligase for NOD2 (K48-linked degradation) and PI3KC2β (inhibiting mast cell signaling) established the paradigm of TRIM27 as a multi-substrate ubiquitin ligase governing innate immune and allergic responses.

    Evidence Co-IP, ubiquitination assays with linkage specificity, Trim27 KO mice with in vivo anaphylaxis model, NF-κB reporter

    PMID:22645315 PMID:22829933

    Open questions at the time
    • Mechanism of substrate selectivity unknown
    • Relative contribution of RING vs scaffold functions not dissected
  6. 2013 High

    Showing that TRIM27 ubiquitinates USP7 to facilitate RIP1 deubiquitination and TNF-α-induced apoptosis, and independently ubiquitinates PTEN with non-degradative chains to inhibit its phosphatase activity, revealed TRIM27 as a versatile ubiquitin ligase employing multiple chain types for distinct functional outcomes.

    Evidence Trim27 KO mice/MEFs for apoptosis, in vitro ubiquitination for PTEN, phosphatase and AKT activation assays

    PMID:23419514 PMID:24144979

    Open questions at the time
    • Chain-type selectivity mechanism undetermined
    • Whether USP7–TRIM27 reciprocal regulation is context-dependent
  7. 2015 High

    The discovery that TRIM27 K48-ubiquitinates TBK1 at defined lysines (K251, K372) downstream of Siglec1/SHP2 recruitment, and that USP7 stabilizes TRIM27 to sustain this function, established TRIM27 as a central negative regulator of type I IFN signaling with a dedicated deubiquitinase partner.

    Evidence Site-directed mutagenesis of TBK1 ubiquitination sites, K48-specific ubiquitin analysis, USP7 KO, IFN-β reporter

    PMID:26358190 PMID:29688809

    Open questions at the time
    • Whether other DUBs can substitute for USP7
    • Viral exploitation of the Siglec1–TRIM27 axis beyond HSV-1
  8. 2018 High

    Localization of TRIM27 to retromer-positive endosomes and demonstration that it scaffolds gp130/JAK1/STAT3 for IL-6-driven STAT3 activation—with in vivo validation in colitis and colitis-associated cancer models—reframed TRIM27 as an endosomal signaling platform beyond its nuclear roles.

    Evidence Confocal localization, Co-IP of signaling complex, Trim27 KO mice in DSS and AOM/DSS models

    PMID:30143645

    Open questions at the time
    • How TRIM27 is targeted to endosomes vs nucleus
    • Whether ligase activity is required for scaffold function at endosomes
  9. 2021 High

    Identification of TRIM27 and USP7 as integral, reciprocally required subunits of the non-canonical PRC1.1 Polycomb complex—where USP7 counteracts TRIM27 autoubiquitination to maintain complex integrity, chromatin binding, and H2AK119ub—unified TRIM27's nuclear chromatin role with its enzymatic activity.

    Evidence Mass spectrometry interactome of USP7, USP7 inhibitor, TRIM27 KD, ChIP for H2AK119ub/H3K27ac, leukemia xenograft

    PMID:34113809

    Open questions at the time
    • Which PRC1.1 target genes depend specifically on TRIM27
    • Structural basis of TRIM27–USP7 within PRC1.1
  10. 2022 High

    Detailed dissection of TRIM27's dual role in autophagy suppression—K48-linked ULK1 degradation basally, plus non-degradative K6/K11-linked ubiquitination of STK38L to activate a phosphorylation-priming step during starvation—revealed an unexpectedly layered ubiquitin-signaling mechanism controlling autophagy flux.

    Evidence In vitro ubiquitination, site-directed mutagenesis of ULK1 K568/K571, Trim27 KO mice, autophagy flux assay, kinase assay

    PMID:35670107

    Open questions at the time
    • Upstream signals switching TRIM27 between chain types
    • Whether TRIM27 autophagy role intersects with its PRC1.1 function
  11. 2022 High

    Demonstrating that TRIM27 is itself an autophagy substrate degraded via ATG7/ATG9-dependent lysosomal pathways, and that it promotes mitophagy by clustering mitochondria via SQSTM1/p62 and recruiting phospho-TBK1, established a feedback loop where TRIM27 both suppresses general autophagy and facilitates selective mitophagy.

    Evidence TRIM27 KO HEK293 cells with reconstitution, autophagy receptor Co-IP, mitophagy flux assay, live imaging

    PMID:36111389

    Open questions at the time
    • Signals distinguishing TRIM27-mediated autophagy suppression from mitophagy promotion
    • Whether TRIM27 mitophagy role is tissue-specific
  12. 2023 High

    Discovery that WNK1 promotes TRIM27–USP7 complex formation and CLEC16A fine-tunes TRIM27 levels on early endosomes established extrinsic regulators of TRIM27's endosomal activity, linking it to WASH-dependent actin dynamics and receptor trafficking.

    Evidence Co-IP, WNK1/TRIM27 KD with endosomal actin and EGFR degradation readouts; CLEC16A MS interactome and CRISPR KO in zebrafish

    PMID:36538041 PMID:37307465

    Open questions at the time
    • How WNK1 kinase activity regulates the complex
    • Whether CLEC16A competes with retromer for TRIM27 binding
  13. 2024 High

    Revealing that upon Mtb infection, nuclear TRIM27 acts as a ligase-independent transcriptional co-activator of TFEB—binding the TFEB promoter and enhancing CREB1 transcriptional activity—uncovered a non-enzymatic gene-regulatory function that drives autophagy-mediated bacterial clearance.

    Evidence ChIP-seq, EMSA, TRIM27 KO mice and cells, autophagy flux and Mtb survival assays, TFEB activator rescue

    PMID:38390831

    Open questions at the time
    • Whether TRIM27 co-activator function extends beyond TFEB/CREB1 targets
    • How infection triggers nuclear retention of TRIM27
    • Structural basis of TRIM27–CREB1 interaction

Open questions

Synthesis pass · forward-looking unresolved questions
  • The mechanism by which TRIM27 is partitioned between its nuclear (PRC1.1 subunit, TFEB co-activator), endosomal (retromer scaffold, WASH regulator), and cytoplasmic (autophagy/mitophagy) compartments—and how context-dependent signals select among its diverse substrates and ubiquitin chain types—remains unresolved.
  • No structural model of full-length TRIM27 or its complexes
  • Signal-dependent compartment switching mechanism unknown
  • Substrate selectivity determinants not defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 11 GO:0016874 ligase activity 5 GO:0003677 DNA binding 2 GO:0140110 transcription regulator activity 2 GO:0060090 molecular adaptor activity 1
Localization
GO:0005634 nucleus 6 GO:0005768 endosome 3 GO:0005829 cytosol 1
Pathway
R-HSA-162582 Signal Transduction 5 R-HSA-168256 Immune System 4 R-HSA-392499 Metabolism of proteins 4 R-HSA-5357801 Programmed Cell Death 3 R-HSA-9612973 Autophagy 3 R-HSA-4839726 Chromatin organization 1
Partners
CLEC16ACREB1EPC1SQSTM1TBK1ULK1USP7WNK1
Complex memberships
PRC1.1 (non-canonical Polycomb repressive complex 1.1)TRIM27-USP7 complex

Evidence

Reading pass · 30 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1992 RFP (TRIM27) protein is strongly associated with the nuclear matrix and has DNA-binding activity, preferring double-stranded over single-stranded DNA, suggesting a role in nuclear matrix-associated molecular processes. Western blot, nuclear matrix fractionation, DNA-binding assay Nucleic acids research Medium 1437549
1996 The RING finger motif of RFP (TRIM27) is critical for the transforming activity of the RFP/RET fusion oncogene; mutation of key cysteine/histidine residues in the RING finger markedly decreases transforming activity and tyrosine phosphorylation, whereas mutations in the B box do not affect activity. Site-directed mutagenesis, NIH 3T3 transformation assay, tyrosine phosphorylation analysis Biochemical and biophysical research communications High 8753810
1997 RFP (TRIM27) homomultimerization occurs through the coiled-coil domain; the B box is not a direct interaction interface but its structural integrity is required for the coiled-coil-mediated interaction. Mutations in the RING finger and B box alter subcellular compartmentalization of RFP. Yeast two-hybrid, mutagenesis, subcellular fractionation/immunofluorescence Journal of cell science High 9247190
1996 Mouse rfp (TRIM27) protein is expressed as nuclear bodies resembling PML oncogenic domains in cell nuclei, with highest expression in pachytene spermatocytes and round spermatids during spermatogenesis. Immunofluorescence, Western blot, in situ hybridization Developmental genetics Medium 9023983
2002 RFP (TRIM27) interacts with Enhancer of Polycomb 1 (EPC1) and functions as a transcriptional repressor; O-glycosylation of the 68-kDa form of RFP (but not the 58-kDa form) is required for its interaction with the C-terminal region of EPC1, and this interaction is responsible for transcriptional repression activity. GST pull-down, co-immunoprecipitation from mouse testis, O-glycosidase treatment, luciferase reporter assay Biochemical and biophysical research communications High 11779184
2012 TRIM27 acts as an E3 ubiquitin ligase that physically interacts with NOD2 via the NOD2 nucleotide-binding domain and promotes K48-linked ubiquitination of NOD2 followed by proteasomal degradation, thereby negatively regulating NOD2-mediated pro-inflammatory signaling. Co-immunoprecipitation, ubiquitination assay, proteasome inhibitor treatment, knockdown/overexpression with NF-κB reporter PloS one High 22829933
2012 TRIM27 negatively regulates IgE receptor (FcεRI) activation of mast cells by ubiquitinating and inhibiting PI3KC2β (class II phosphatidylinositol-3-kinase C2β), thereby suppressing KCa3.1 channel activation, Ca2+ influx, cytokine production, and degranulation; TRIM27−/− mice show enhanced anaphylaxis. Knockout mice, bone marrow-derived mast cell assays, Co-immunoprecipitation, ubiquitination assay, in vivo anaphylaxis model Molecular and cellular biology High 22645315
2013 TRIM27 forms a complex with the deubiquitinase USP7; TRIM27 ubiquitinates USP7, which in turn deubiquitinates RIP1, resulting in positive regulation of TNF-α-induced apoptosis. Trim27-deficient mice and MEFs are resistant to TNF-α-induced hepatocyte/fibroblast apoptosis. Knockout mice, MEF cell assays, Co-immunoprecipitation, ubiquitination assay, in vivo TNF-α/D-galactosamine model Molecular and cellular biology High 24144979
2013 RFP (TRIM27) acts as an E3 ubiquitin ligase for PTEN, promoting atypical polyubiquitination of PTEN that does not lead to degradation or relocalization but significantly inhibits PTEN phosphatase activity, thereby enhancing AKT activation and suppressing PTEN-mediated apoptosis. In vitro ubiquitination assay, RNAi knockdown, overexpression, phosphatase activity assay, AKT activation assay Cell research High 23419514
2014 HSV-1 immediate-early protein ICP0, via its RING domain E3 ligase activity, targets TRIM27 for rapid proteasomal degradation during infection; a RING-mutant ICP0 co-immunoprecipitates endogenous TRIM27, demonstrating direct interaction. Proteomic screen, reciprocal co-immunoprecipitation, proteasome inhibitor rescue, immunofluorescence, ICP0 RING mutant analysis Journal of virology High 25320289
2014 TRIM27 forms a complex with MRTF-B and posttranscriptionally regulates integrin β1 expression in leading cancer cells by stabilizing integrin β1 mRNA via microRNA-124 suppression, driving collective cancer cell invasion. Co-immunoprecipitation, knockdown, invasion assays in vitro and in vivo, mRNA stability assay, microRNA analysis Cell reports High 24794433
2015 Siglec1 recruits SHP2, which then recruits TRIM27; TRIM27 promotes K48-linked ubiquitination of TBK1 at Lys251 and Lys372, leading to TBK1 proteasomal degradation and suppression of type I IFN production during viral infection. Co-immunoprecipitation, ubiquitination assay with K48 linkage-specific analysis, site-directed mutagenesis of TBK1, knockdown/overexpression Cell research High 26358190
2015 USP7 physically interacts with TRIM27, stabilizes TRIM27 protein, and promotes TRIM27-mediated ubiquitination and degradation of TBK1, thereby negatively regulating type I IFN antiviral signaling; knockout of USP7 leads to enhanced TRIM27 degradation and reduced TBK1 ubiquitination. Co-immunoprecipitation, knockout/knockdown, ubiquitination assay, IFN-β reporter assay FASEB journal High 29688809
2016 TRIM27 restricts intracellular mycobacterial survival in macrophages by promoting innate immune responses and cell apoptosis via the JNK/p38 MAPK pathway; Mtb PtpA antagonizes TRIM27 function by competitively binding to the RING domain of TRIM27. Co-immunoprecipitation, knockdown/overexpression, intracellular bacterial survival assay, MAPK pathway analysis, competitive binding assay Scientific reports Medium 27698396
2016 Trim27 interacts with Slx2 and co-localizes to the XY body during meiosis in mouse spermatocytes; Trim27 also co-localizes with γ-H2AX in the XY body, suggesting a role as a transcriptional regulator in meiotic XY body formation and spermatogenesis. Yeast two-hybrid, co-immunoprecipitation, mammalian two-hybrid, immunohistochemistry, cytoimmunofluorescence Cell cycle Medium 27612028
2018 TRIM27 localizes to retromer-positive punctate endosomal structures and serves as a scaffolding platform recruiting gp130, JAK1, and STAT3, leading to STAT3 phosphorylation at retromer-positive structures; TRIM27 deficiency impairs IL-6-induced STAT3 activation, inflammatory cytokine expression, and DSS-induced colitis and AOM/DSS-induced colitis-associated cancer in mice. Immunofluorescence/confocal localization, Co-immunoprecipitation, knockdown/overexpression, mouse KO models (DSS colitis, AOM/DSS), tumor xenograft Nature communications High 30143645
2018 TRIM27 interacts with PIAS3 and promotes PIAS3 ubiquitination, leading to enhanced STAT3 activation, increased keratinocyte proliferation and inflammatory cytokine production in response to IL-6. Co-immunoprecipitation, ubiquitination assay, knockdown/overexpression, cell proliferation assay, ELISA American journal of physiology. Cell physiology Medium 31747314
2020 TRIM27 acts as an E3 ubiquitin ligase for p21 (CDKN1A), mediating its ubiquitination and degradation to suppress cellular senescence in non-triple-negative breast cancer cells. Co-immunoprecipitation, ubiquitination assay, knockdown/overexpression with senescence assays Molecular therapy. Nucleic acids Medium 33251042
2020 TRIM27 ubiquitinates SIX3 to promote its degradation, thereby activating the Wnt/β-catenin signaling pathway and promoting NSCLC cell proliferation and metastasis. Co-immunoprecipitation, ubiquitination assay, knockdown/overexpression, proliferation and invasion assays, β-catenin inhibitor rescue Aging Medium 33264103
2020 TRIM27 ubiquitinates and degrades PPARγ, leading to increased cleaved Caspase-3 and IL-1β expression, thereby promoting glutamate-induced neuronal apoptosis and inflammation. Co-immunoprecipitation, ubiquitination assay, knockdown/overexpression, PPARγ agonist rescue Experimental cell research Medium 33385414
2021 TRIM27 and USP7 are integral components of the non-canonical PRC1.1 Polycomb complex; USP7 and TRIM27 are reciprocally required for incorporation into PRC1.1, and USP7 counteracts TRIM27 E3 ligase activity to maintain PRC1.1 integrity, chromatin binding, H2AK119ub levels, and active gene transcription. Co-immunoprecipitation/USP7 interactome mass spectrometry, USP7 inhibitor treatment, TRIM27 knockdown, chromatin immunoprecipitation (H2AK119ub, H3K27ac), gene expression analysis, in vivo leukemia xenograft iScience High 34113809
2021 TRIM27 directly interacts with IκBα and mediates its ubiquitination, leading to NF-κB pathway activation and promotion of renal cancer cell proliferation. Co-immunoprecipitation, ubiquitination assay, NF-κB activity assay, knockdown/overexpression BMC cancer Medium 34284744
2021 TRIM27 interacts with p53 and promotes its ubiquitination and degradation; TRIM27 deficiency (cardiac conditional knockout) exacerbates MI/R injury by enhancing p53-mediated apoptosis and NF-κB-driven inflammation. Cardiac conditional knockout mice, Co-immunoprecipitation, ubiquitination assay, p53 inhibitor rescue, in vivo MI/R model Biochemical and biophysical research communications Medium 33865220
2022 TRIM27 functions as a negative regulator of autophagy by directly polyubiquitinating ULK1 at K568 and K571 with K48-linked chains for proteasomal degradation (basal control) and by catalyzing non-degradative K6/K11-linked ubiquitination of STK38L, activating STK38L to phosphorylate ULK1 at Ser495 and priming ULK1 for further TRIM27-mediated hyper-ubiquitination during starvation; Trim27 knockout mice show increased basal autophagy. In vitro ubiquitination assay, site-directed mutagenesis of ULK1 ubiquitination sites, Co-immunoprecipitation, Trim27 knockout mice, autophagy flux assay, kinase assay The EMBO journal High 35670107
2022 TRIM27 is an autophagy substrate degraded via the lysosomal pathway in an ATG7-, ATG9-, and autophagy receptor-dependent manner; TRIM27 forms ubiquitylated cytoplasmic bodies co-localizing with autophagy receptors; TRIM27 mediates mitochondrial clustering via SQSTM1/p62 interaction; phosphorylated TBK1 is recruited to TRIM27-clustered mitochondria; TRIM27 loss reduces mitophagy, which is fully restored by TRIM27 re-expression. TRIM27 KO cells (HEK293 FlpIn), EGFP-TRIM27 reconstitution, autophagy receptor Co-IP, live imaging, mitophagy flux assay, TBK1 inhibitor treatment, immunofluorescence co-localization The FEBS journal High 36111389
2023 TRIM27 stabilizes β-catenin to activate Wnt/β-catenin signaling, promoting intestinal stem cell self-renewal; Trim27 KO mice show IBS-like symptoms and impaired organoid formation that is rescued by TRIM27 re-expression or β-catenin restoration. Trim27 knockout mice, organoid formation assay, rescue with TRIM27/β-catenin re-expression, Wnt activator treatment, Western blot Cellular & molecular immunology High 36596873
2023 WNK1 interacts with TRIM27 and promotes the TRIM27-USP7 complex; WNK1 knockdown reduces TRIM27-USP7 complex formation, decreases TRIM27 protein levels, disrupts WASH complex ubiquitination and endosomal actin polymerization, and impairs endosomal trafficking; depletion of either WNK1 or TRIM27 increases EGFR degradation following ligand stimulation. Co-immunoprecipitation, WNK1/TRIM27 knockdown, endosomal actin assay, EGFR degradation assay Proceedings of the National Academy of Sciences of the United States of America High 37307465
2024 Upon Mtb infection, nuclear-localized TRIM27 acts as a transcription activator for TFEB by binding to the TFEB promoter and to the transcription factor CREB1, enhancing CREB1-TFEB promoter binding affinity and CREB1 transcriptional activity, thereby inducing autophagy-related gene expression and autophagy flux to clear intracellular Mtb; this function is independent of TRIM27's E3 ligase activity. ChIP-seq, EMSA, Co-immunoprecipitation, TRIM27 KO mice and cells, autophagy flux assay, intracellular Mtb survival assay, TFEB activator rescue Autophagy High 38390831
2022 TRIM27 interacts with KLF12 at its N-terminal region and ubiquitinates KLF12 at K326 via K33-linked polyubiquitination, reducing KLF12 transcriptional repressor activity toward L1CAM and thereby promoting cisplatin resistance and cancer metastasis in ESCC. Co-immunoprecipitation, ubiquitination assay with linkage specificity, site-directed mutagenesis of K326, L1CAM promoter reporter assay Drug resistance updates High 38924996
2022 CLEC16A interacts with retromer complex subunits and with TRIM27 at early endosomes; CLEC16A loss increases TRIM27 adhesion to early endosomes and causes abnormal accumulation of endosomal F-actin, indicating that CLEC16A regulates endosomal fate by fine-tuning levels of TRIM27 on the endosome surface. Mass spectrometry interactome, Co-immunoprecipitation, immunofluorescence localization, CLEC16A KD/truncation mutant, zebrafish CRISPR KO, endosomal actin assay Human genetics High 36538041

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 New rfp- and pES213-derived tools for analyzing symbiotic Vibrio fischeri reveal patterns of infection and lux expression in situ. Applied and environmental microbiology 224 16391121
2015 Siglec1 suppresses antiviral innate immune response by inducing TBK1 degradation via the ubiquitin ligase TRIM27. Cell research 159 26358190
2004 New Drosophila transgenic reporters: insulated P-element vectors expressing fast-maturing RFP. BioTechniques 156 15038159
2014 Mapping early fate determination in Lgr5+ crypt stem cells using a novel Ki67-RFP allele. The EMBO journal 128 25092767
2019 An Id2RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials. Immunity 127 30926235
1994 Two Mhc class I and two Mhc class II genes map to the chicken Rfp-Y system outside the B complex. Proceedings of the National Academy of Sciences of the United States of America 122 7910407
1996 Assignment of Rfp-Y to the chicken major histocompatibility complex/NOR microchromosome and evidence for high-frequency recombination associated with the nucleolar organizer region. Proceedings of the National Academy of Sciences of the United States of America 116 8632997
2012 TRIM27 negatively regulates NOD2 by ubiquitination and proteasomal degradation. PloS one 101 22829933
2018 Up-regulation of miR-383-5p suppresses proliferation and enhances chemosensitivity in ovarian cancer cells by targeting TRIM27. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 100 30399596
2013 Ubiquitination-deubiquitination by the TRIM27-USP7 complex regulates tumor necrosis factor alpha-induced apoptosis. Molecular and cellular biology 99 24144979
2018 Molecular Profiling of Salivary Gland Intraductal Carcinoma Revealed a Subset of Tumors Harboring NCOA4-RET and Novel TRIM27-RET Fusions: A Report of 17 cases. The American journal of surgical pathology 95 30045065
1997 Involvement of the rfp tripartite motif in protein-protein interactions and subcellular distribution. Journal of cell science 95 9247190
2019 NCOA4-RET and TRIM27-RET Are Characteristic Gene Fusions in Salivary Intraductal Carcinoma, Including Invasive and Metastatic Tumors: Is "Intraductal" Correct? The American journal of surgical pathology 93 31162284
1992 Role of Escherichia coli K-12 rfa genes and the rfp gene of Shigella dysenteriae 1 in generation of lipopolysaccharide core heterogeneity and attachment of O antigen. Journal of bacteriology 77 1385388
2018 TRIM27 mediates STAT3 activation at retromer-positive structures to promote colitis and colitis-associated carcinogenesis. Nature communications 68 30143645
2018 TRIM27 functions as an oncogene by activating epithelial-mesenchymal transition and p-AKT in colorectal cancer. International journal of oncology 65 29767249
2001 At least one class I gene in restriction fragment pattern-Y (Rfp-Y), the second MHC gene cluster in the chicken, is transcribed, polymorphic, and shows divergent specialization in antigen binding region. Journal of immunology (Baltimore, Md. : 1950) 65 11207288
2013 RFP-mediated ubiquitination of PTEN modulates its effect on AKT activation. Cell research 62 23419514
2002 Three novel human VMD2-like genes are members of the evolutionary highly conserved RFP-TM family. European journal of human genetics : EJHG 62 12032738
2016 The ubiquitin ligase TRIM27 functions as a host restriction factor antagonized by Mycobacterium tuberculosis PtpA during mycobacterial infection. Scientific reports 61 27698396
2009 Transthyretin mouse transgenes direct RFP expression or Cre-mediated recombination throughout the visceral endoderm. Genesis (New York, N.Y. : 2000) 61 19415627
1992 RFP is a DNA binding protein associated with the nuclear matrix. Nucleic acids research 56 1437549
2022 TRIM27 cooperates with STK38L to inhibit ULK1-mediated autophagy and promote tumorigenesis. The EMBO journal 53 35670107
2017 DNA mimics of red fluorescent proteins (RFP) based on G-quadruplex-confined synthetic RFP chromophores. Nucleic acids research 53 28981852
2004 Cloning and characterization of the murine Vmd2 RFP-TM gene family. Cytogenetic and genome research 53 15218265
2009 Simultaneous determination of labile proton concentration and exchange rate utilizing optimal RF power: Radio frequency power (RFP) dependence of chemical exchange saturation transfer (CEST) MRI. Journal of magnetic resonance (San Diego, Calif. : 1997) 52 19926319
2005 Localization and functional interrelationships among cytosolic Group IV, secreted Group V, and Ca2+-independent Group VI phospholipase A2s in P388D1 macrophages using GFP/RFP constructs. Biochimica et biophysica acta 52 15967714
2019 TRIM27 promotes IL-6-induced proliferation and inflammation factor production by activating STAT3 signaling in HaCaT cells. American journal of physiology. Cell physiology 50 31747314
2018 USP7-TRIM27 axis negatively modulates antiviral type I IFN signaling. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 47 29688809
2015 Downregulation of TRIM27 expression inhibits the proliferation of ovarian cancer cells in vitro and in vivo. Laboratory investigation; a journal of technical methods and pathology 47 26568293
1996 Association between the Rfp-Y haplotype and the incidence of Marek's disease in chickens. Immunogenetics 47 8753853
2019 TRIM27 promotes the development of esophagus cancer via regulating PTEN/AKT signaling pathway. Cancer cell international 46 31719796
2003 Novel tumorigenic rearrangement, Delta rfp/ret, in a papillary thyroid carcinoma from externally irradiated patient. Mutation research 46 12787916
2002 Improved version of the red fluorescent protein (drFP583/DsRed/RFP). BioTechniques 43 12238769
1996 Mapping of the genetically independent chicken major histocompatibility complexes B@ and RFP-Y@ to the same microchromosome by two-color fluorescent in situ hybridization. Cytogenetics and cell genetics 43 8995478
1986 Cloning of the rfb gene region of Shigella dysenteriae 1 and construction of an rfb-rfp gene cassette for the development of lipopolysaccharide-based live anti-dysentery vaccines. Microbial pathogenesis 43 2469931
1996 Mouse ret finger protein (rfp) proto-oncogene is expressed at specific stages of mouse spermatogenesis. Developmental genetics 42 9023983
2015 Single-molecule microscopy of molecules tagged with GFP or RFP derivatives in mammalian cells using nanobody binders. Methods (San Diego, Calif.) 41 26123185
2016 Two-step cultivation for production of astaxanthin in Chlorella zofingiensis using a patented energy-free rotating floating photobioreactor (RFP). Bioresource technology 39 27818161
2014 Identification of TRIM27 as a novel degradation target of herpes simplex virus 1 ICP0. Journal of virology 39 25320289
2008 Zebrafish primordial germ cell cultures derived from vasa::RFP transgenic embryos. Stem cells and development 39 18576915
2003 Analysis of part of the chicken Rfp-Y region reveals two novel lectin genes, the first complete genomic sequence of a class I alpha-chain gene, a truncated class II beta-chain gene, and a large CR1 repeat. Immunogenetics 39 12692693
2020 TRIM27 Functions as a Novel Oncogene in Non-Triple-Negative Breast Cancer by Blocking Cellular Senescence through p21 Ubiquitination. Molecular therapy. Nucleic acids 38 33251042
2015 Type I IFN-Inducible Downregulation of MicroRNA-27a Feedback Inhibits Antiviral Innate Response by Upregulating Siglec1/TRIM27. Journal of immunology (Baltimore, Md. : 1950) 37 26700765
2011 Role of IncP-1β plasmids pWDL7::rfp and pNB8c in chloroaniline catabolism as determined by genomic and functional analyses. Applied and environmental microbiology 37 22101050
2014 TRIM27/MRTF-B-dependent integrin β1 expression defines leading cells in cancer cell collectives. Cell reports 36 24794433
2006 Tumor cells genetically labeled with GFP in the nucleus and RFP in the cytoplasm for imaging cellular dynamics. Cell cycle (Georgetown, Tex.) 36 16760659
1996 Histocompatibility antigen(s) linked to Rfp-Y (Mhc-like) genes in the chicken. Immunogenetics 35 8881037
2013 Distribution of MT1 melatonin receptor promoter-driven RFP expression in the brains of BAC C3H/HeN transgenic mice. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 33 24051358
1996 A RING finger motif regulates transforming activity of the rfp/ret fusion gene. Biochemical and biophysical research communications 33 8753810
2021 The USP7-TRIM27 axis mediates non-canonical PRC1.1 function and is a druggable target in leukemia. iScience 32 34113809
1994 Lipopolysaccharide O-antigen biosynthesis in Shigella dysenteriae serotype 1: analysis of the plasmid-carried rfp determinant. Microbial pathogenesis 31 7520113
2020 TRIM27 acts as an oncogene and regulates cell proliferation and metastasis in non-small cell lung cancer through SIX3-β-catenin signaling. Aging 30 33264103
2012 Phosphatidylinositol-3-kinase C2β and TRIM27 function to positively and negatively regulate IgE receptor activation of mast cells. Molecular and cellular biology 30 22645315
2019 Intraductal Carcinoma of Salivary Glands Harboring TRIM27-RET Fusion with Mixed Low Grade and Apocrine Types. Head and neck pathology 28 30610524
2022 TRIM27-USP7 complex promotes tumour progression via STAT3 activation in human hepatocellular carcinoma. Liver international : official journal of the International Association for the Study of the Liver 27 35753056
2019 Knockdown of TRIM27 expression suppresses the dysfunction of mesangial cells in lupus nephritis by FoxO1 pathway. Journal of cellular physiology 26 30648253
2015 Red fluorescent proteins (RFPs) and RFP-based biosensors for neuronal imaging applications. Neurophotonics 26 26158012
2023 TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal. Cellular & molecular immunology 25 36596873
2022 Emerging roles of TRIM27 in cancer and other human diseases. Frontiers in cell and developmental biology 25 36200036
2021 TRIM27 interacts with Iκbα to promote the growth of human renal cancer cells through regulating the NF-κB pathway. BMC cancer 24 34284744
2012 Fine mapping and candidate gene analysis of the nuclear restorer gene Rfp for pol CMS in rapeseed (Brassica napus L.). TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 23 22614178
2002 Generation of two-color transgenic zebrafish using the green and red fluorescent protein reporter genes gfp and rfp. Marine biotechnology (New York, N.Y.) 22 14961274
1997 Non-association between Rfp-Y major histocompatibility complex-like genes and susceptibility to Marek's disease virus-induced tumours in 6(3) x 7(2) F2 intercross chickens. Animal genetics 22 9363593
2023 TRIM27 ameliorates ischemic stroke by regulating NLRP3 inflammasome-mediated pyroptosis via the Akt/Nrf2/HO-1 signaling. Experimental neurology 21 37914066
2015 Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation. Biology of reproduction 21 26607717
2006 Characterization of the Interleukin (IL)-6 Inhibitor IL-6-RFP: fused receptor domains act as high affinity cytokine-binding proteins. The Journal of biological chemistry 21 17085445
2024 TRIM27 elicits protective immunity against tuberculosis by activating TFEB-mediated autophagy flux. Autophagy 20 38390831
2020 TRIM27-mediated ubiquitination of PPARγ promotes glutamate-induced cell apoptosis and inflammation. Experimental cell research 20 33385414
2006 A keratin 18 transgenic zebrafish Tg(k18(2.9):RFP) treated with inorganic arsenite reveals visible overproliferation of epithelial cells. Toxicology letters 20 16376500
2006 Spatiotemporal expression of zebrafish keratin 18 during early embryogenesis and the establishment of a keratin 18:RFP transgenic line. Gene expression patterns : GEP 20 16446122
2021 TRIM27 contributes to glomerular endothelial cell injury in lupus nephritis by mediating the FoxO1 signaling pathway. Laboratory investigation; a journal of technical methods and pathology 19 33854173
2020 Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing. PloS one 19 31999769
2019 TRIM27 Promotes Hepatitis C Virus Replication by Suppressing Type I Interferon Response. Inflammation 19 30847745
2021 TRIM27 protects against cardiac ischemia-reperfusion injury by suppression of apoptosis and inflammation via negatively regulating p53. Biochemical and biophysical research communications 18 33865220
2021 Circ_0091579 exerts an oncogenic role in hepatocellular carcinoma via mediating miR-136-5p/TRIM27. Biomedical journal 18 34974169
2019 Characterization of Tg(Etv4-GFP) and Etv5 Reporter Lines in the Context of Fibroblast Growth Factor 10 Signaling During Mouse Embryonic Lung Development. Frontiers in genetics 18 30923534
2014 Deficiency of Trim27 protects dopaminergic neurons from apoptosis in the neurotoxin model of Parkinson's disease. Brain research 18 25223908
2003 Genotypic variability at the major histocompatibility complex (B and Rfp-Y) in Camperos broiler chickens. Animal genetics 18 12648091
2022 Subcellular localization of mutant P23H rhodopsin in an RFP fusion knock-in mouse model of retinitis pigmentosa. Disease models & mechanisms 17 35275162
2020 MicroRNA‑1 regulates the development of osteoarthritis in a Col2a1‑Cre‑ERT2/GFPfl/fl‑RFP‑miR‑1 mouse model of osteoarthritis through the downregulation of Indian hedgehog expression. International journal of molecular medicine 17 32626917
2016 Trim27 interacts with Slx2, is associated with meiotic processes during spermatogenesis. Cell cycle (Georgetown, Tex.) 17 27612028
2016 An enclosed rotating floating photobioreactor (RFP) powered by flowing water for mass cultivation of photosynthetic microalgae. Biotechnology for biofuels 17 27777620
2006 Common bile duct injection as a novel method for establishing red fluorescent protein (RFP)-expressing human pancreatic cancer in nude mice. JOP : Journal of the pancreas 17 16525203
2022 TRIM27 suppresses inflammation injuries in pediatric pneumonia by targeting TLR4/NF-κB signaling pathway. Allergologia et immunopathologia 16 35257543
2023 WNK1 controls endosomal trafficking through TRIM27-dependent regulation of actin assembly. Proceedings of the National Academy of Sciences of the United States of America 15 37307465
2022 TRIM27 is an autophagy substrate facilitating mitochondria clustering and mitophagy via phosphorylated TBK1. The FEBS journal 15 36111389
2020 Downregulation of TRIM27 suppresses gastric cancer cell proliferation via inhibition of the Hippo-BIRC5 pathway. Pathology, research and practice 15 32825933
2016 Development and Validation of a Rex1-RFP Potency Activity Reporter Assay That Quantifies Stress-Forced Potency Loss in Mouse Embryonic Stem Cells. Stem cells and development 15 26651054
1997 New chicken Rfp-Y haplotypes on the basis of MHC class II RFLP and MLC analyses. Immunogenetics 15 9089091
2022 CLEC16A interacts with retromer and TRIM27, and its loss impairs endosomal trafficking and neurodevelopment. Human genetics 14 36538041
2015 Imaging the microenvironment of pancreatic cancer patient-derived orthotopic xenografts (PDOX) growing in transgenic nude mice expressing GFP, RFP, or CFP. Cancer letters 14 26742463
2008 Generation of neuronal-like cells from umbilical cord blood-derived mesenchymal stem cells of a RFP-transgenic cloned cat. The Journal of veterinary medical science 14 18685247
1998 Rfp-Y region polymorphism and Marek's disease resistance in multitrait immunocompetence-selected chicken lines. Poultry science 14 9565235
1987 Restriction fragment pattern (RFP) analysis of genomes from Danish isolates of suid herpesvirus 1 (Aujezsky's disease virus). Archives of virology 14 2827607
2024 KLF12 interacts with TRIM27 to affect cisplatin resistance and cancer metastasis in esophageal squamous cell carcinoma by regulating L1CAM expression. Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 13 38924996
2022 Knockdown of TRIM27 alleviated sepsis-induced inflammation, apoptosis, and oxidative stress via suppressing ubiquitination of PPARγ and reducing NOX4 expression. Inflammation research : official journal of the European Histamine Research Society ... [et al.] 13 35962797
2002 Role for O-glycosylation of RFP in the interaction with enhancer of polycomb. Biochemical and biophysical research communications 13 11779184
2015 Duck TRIM27-L enhances MAVS signaling and is absent in chickens and turkeys. Molecular immunology 12 26254985
2003 Transcriptional control of orf224/atp6 by the pol CMS restorer Rfp gene in Brassica napus L. Yi chuan xue bao = Acta genetica Sinica 11 12924163