Affinage

TRIM27

Zinc finger protein RFP · UniProt P14373

Length
513 aa
Mass
58.5 kDa
Annotated
2026-06-10
100 papers in source corpus 32 papers cited in narrative 32 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

TRIM27 (RFP/RNF76) is a RING-domain E3 ubiquitin ligase that multimerizes through its coiled-coil domain and acts across nuclear chromatin and endosomal compartments to control immune signaling, autophagy, and cell-fate programs largely by ubiquitinating substrates for degradation or functional modulation (PMID:9247190, PMID:22829933). Its RING finger is essential for catalytic and transforming activity, while the B-box is dispensable for catalysis but required for proper multimerization and subcellular compartmentalization (PMID:9247190, PMID:8753810). As a negative regulator of innate and inflammatory signaling, TRIM27 drives K48-linked ubiquitination and proteasomal degradation of TBK1 and NOD2, suppressing type I IFN and pro-inflammatory output, and ubiquitinates PI3KC2β to restrain FcεRI signaling in mast cells (PMID:22829933, PMID:22645315, PMID:26358190). It restrains autophagy amplitude by polyubiquitinating ULK1 (K48-linked, at K568/K571) and by attaching non-degradative K6/K11 chains to STK38L, which activates STK38L to phosphorylate ULK1 and amplify its TRIM27-dependent ubiquitination (PMID:35670107). TRIM27 also targets PTEN through atypical polyubiquitination that inhibits phosphatase activity to relieve AKT, and degrades p21, p53, PPARγ, IκBα, PIAS3, SIX3, and KLF12 to promote proliferation, survival, NF-κB/STAT3/β-catenin signaling, and drug resistance in diverse cancers (PMID:23419514, PMID:33264103, PMID:34284744, PMID:38924996). Independent of its ligase activity, nuclear TRIM27 is a transcriptional activator of the autophagy master regulator TFEB, binding the TFEB promoter together with CREB1 (PMID:38390831), and it associates with the non-canonical PRC1.1 Polycomb complex where its activity is counterbalanced by USP7 (PMID:34113809). At retromer-positive endosomal structures, TRIM27 is stabilized by USP7 and scaffolds gp130/JAK1/STAT3 to promote STAT3 activation, while its endosomal levels and WASH-complex-dependent actin dynamics are controlled by CLEC16A and WNK1 (PMID:30143645, PMID:29688809, PMID:36538041, PMID:37307465). TRIM27 additionally stabilizes phospho-TBK1 on mitochondria to promote mitophagy and is itself an autophagy substrate via SQSTM1/p62 (PMID:36111389).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1992 Medium

    Established the earliest biochemical character of the protein—that it is a nuclear-matrix-associated, DNA-binding factor with a testis-specific isoform—framing it as a chromatin-linked regulator.

    Evidence Nuclear matrix fractionation, Western blot, and in vitro DNA-binding assay

    PMID:1437549

    Open questions at the time
    • No sequence-specific DNA targets identified
    • Functional consequence of DNA binding unknown
  2. 1996 Medium

    Defined the domain architecture underlying activity: the coiled-coil drives homomultimerization, the B-box supports multimerization and localization, and the RING is required for transforming/catalytic activity.

    Evidence Yeast two-hybrid, deletion/site-directed mutagenesis, NIH 3T3 transformation and localization assays

    PMID:8753810 PMID:9247190

    Open questions at the time
    • RING-dependent ubiquitin ligase activity not yet demonstrated directly
    • Transforming activity studied in the RFP/RET fusion context
  3. 2002 Medium

    Linked TRIM27 to transcriptional repression and Polycomb-type machinery through interaction with EPC1, with isoform glycosylation tuning the interaction.

    Evidence GST pull-down, Co-IP from mouse testis, O-glycosidase treatment, luciferase reporter

    PMID:11779184

    Open questions at the time
    • Target genes of repression not mapped
    • Mechanism of repression not resolved
  4. 2005 Medium

    Showed TRIM27 represses bHLH transcription factors (SCL, E47, MyoD, mASH-1) via HDAC-sensitive, non-degradative means, distinguishing a transcriptional repressor function from its ligase activity.

    Evidence Co-IP, domain mapping, luciferase reporter, trichostatin A treatment

    PMID:16007160

    Open questions at the time
    • Direct HDAC/Polycomb recruitment not biochemically defined
    • Physiological developmental role untested
  5. 2012 High

    Established TRIM27 as a bona fide E3 ligase that negatively regulates innate immune signaling by K48-ubiquitinating NOD2 for degradation and by inhibiting PI3KC2β in mast cells, with an in vivo anaphylaxis phenotype.

    Evidence Co-IP, in-cell ubiquitination assays, NF-κB reporter, TRIM27-/- mouse and in vivo anaphylaxis model

    PMID:22645315 PMID:22829933

    Open questions at the time
    • Linkage chemistry on PI3KC2β not fully defined
    • Recruitment mechanism to substrates unresolved
  6. 2013 High

    Connected TRIM27 to apoptosis and PTEN/AKT regulation: it partners with USP7 to control TNF-α-induced apoptosis via RIP1, and atypically ubiquitinates PTEN to inhibit its phosphatase activity without degradation.

    Evidence Reciprocal Co-IP, ubiquitination and phosphatase assays, Trim27-/- MEFs and mice, TNF-α apoptosis assay

    PMID:23419514 PMID:24144979

    Open questions at the time
    • Ubiquitin chain type on PTEN not fully characterized
    • Interplay of USP7 with TRIM27 catalysis incompletely defined at this stage
  7. 2014 Medium

    Revealed TRIM27 as both a viral target and a context-dependent host factor: HSV-1 ICP0 degrades TRIM27, yet TRIM27 can positively support infection; separately TRIM27/MRTF-B promote collective cancer invasion via integrin β1.

    Evidence Proteomic screen, Co-IP, proteasome inhibition, RING-mutant ICP0, invasion assays, miRNA reporter

    PMID:24794433 PMID:25320289

    Open questions at the time
    • Pro- versus anti-viral balance mechanistically unresolved
    • How MRTF-B/TRIM27 suppress miR-124 not defined
  8. 2015 High

    Defined the mechanism by which TRIM27 suppresses antiviral IFN: recruited via Siglec1-DAP12-SHP2, it K48-ubiquitinates TBK1 at K251/K372 for degradation.

    Evidence Co-IP, linkage-specific ubiquitination, proteasome inhibitor, site-directed mutagenesis of TBK1, RNAi

    PMID:26358190

    Open questions at the time
    • Spatial site of TBK1 ubiquitination not localized
    • Regulation of the recruitment axis incompletely defined
  9. 2016 Medium

    Extended TRIM27 functions to meiotic chromatin (XY body, with Slx2 and γ-H2AX) and to anti-mycobacterial immunity via JNK/p38 MAPK, with the Mtb effector PtpA antagonizing the RING domain.

    Evidence Yeast/mammalian two-hybrid, Co-IP, immunohistochemistry, macrophage infection and MAPK analysis

    PMID:27612028 PMID:27698396

    Open questions at the time
    • Functional role in XY body formation not genetically tested
    • Substrates relevant to MAPK activation not identified
  10. 2018 High

    Identified the endosomal scaffolding function and a stability-control loop: at retromer puncta TRIM27 assembles gp130/JAK1/STAT3 to drive STAT3 activation, while USP7 stabilizes TRIM27 to sustain TBK1 degradation and IFN suppression.

    Evidence Co-IP, subcellular fractionation/IF, knockdown with STAT3 readout, DSS/AOM colitis model, USP7 KO with IFN reporter

    PMID:29688809 PMID:30143645

    Open questions at the time
    • How scaffolding versus ligase functions are partitioned spatially unclear
    • Determinants of retromer recruitment undefined
  11. 2019 Medium

    Showed TRIM27 amplifies STAT3 signaling and AKT signaling in epithelial/cancer contexts by degrading the STAT3 inhibitor PIAS3 and ubiquitinating PTEN.

    Evidence Co-IP, ubiquitination assays, overexpression/silencing, AKT inhibitor rescue, STAT3 phosphorylation readouts

    PMID:31719796 PMID:31747314

    Open questions at the time
    • Chain linkage on PIAS3 not defined
    • Tissue specificity of these effects not established
  12. 2020 Medium

    Broadened the degradative substrate repertoire driving proliferation/survival programs: TRIM27 degrades SIX3 (activating β-catenin targets), p21 (suppressing senescence), and PPARγ (promoting neuronal apoptosis).

    Evidence Co-IP, ubiquitination assays, knockdown/overexpression, pharmacological rescues (XAV939, rosiglitazone)

    PMID:33251042 PMID:33264103 PMID:33385414

    Open questions at the time
    • Direct versus indirect ubiquitination not uniformly established
    • Substrate selection determinants unknown
  13. 2021 Medium

    Placed TRIM27 in the non-canonical PRC1.1 Polycomb complex (reciprocally with USP7) and connected it to NF-κB and p53 control in disease models, defining roles in chromatin regulation and tissue protection.

    Evidence Co-IP/interactome, ChIP and histone-mark analyses, USP7 inhibitor; ubiquitination assays with NF-κB reporter; heart-conditional KO MI/R model

    PMID:33865220 PMID:34113809 PMID:34284744

    Open questions at the time
    • How PRC1.1 chromatin role relates to cytoplasmic ligase functions unclear
    • Direct catalytic role within PRC1.1 versus structural role unresolved
  14. 2022 High

    Resolved a multi-layered autophagy-restraint mechanism and a mitophagy role: TRIM27 K48-ubiquitinates ULK1 (K568/K571) and K6/K11-ubiquitinates STK38L to set up a phosphorylation relay, while stabilizing phospho-TBK1 on mitochondria; TRIM27 is itself degraded by autophagy via p62.

    Evidence In vitro ubiquitination reconstitution, site-directed mutagenesis, kinase assay, KO mice and KO-cell rescue, mitophagy/autophagy flux assays

    PMID:35670107 PMID:36111389

    Open questions at the time
    • Signals switching TRIM27 between substrates not defined
    • Coordination of mitochondrial versus cytosolic pools unclear
  15. 2023 Medium

    Defined endosomal regulators and a tissue-homeostasis role: CLEC16A and WNK1 control endosomal TRIM27 levels, WASH-complex ubiquitination, and EGFR trafficking, while TRIM27 stabilizes β-catenin to support intestinal stem cell self-renewal.

    Evidence MS interactome, Co-IP, knockdowns with EGFR/actin readouts; Trim27 KO mice and organoid rescue with β-catenin

    PMID:36538041 PMID:36596873 PMID:37307465

    Open questions at the time
    • Mechanism by which TRIM27 stabilizes β-catenin not defined
    • WASH substrate(s) of TRIM27 at endosomes not pinpointed
  16. 2024 High

    Established a ligase-independent transcriptional function—nuclear TRIM27 activates TFEB with CREB1 to promote autophagy against Mtb—and a K33-linked ubiquitination of KLF12 promoting drug resistance, distinguishing catalytic from non-catalytic outputs.

    Evidence ChIP-seq, EMSA, Co-IP, KO cell/mouse, autophagy flux; linkage-specific ubiquitination and cisplatin-resistance assays

    PMID:38390831 PMID:38924996

    Open questions at the time
    • Switch between nuclear transcriptional and cytoplasmic ligase roles unresolved
    • Determinants of K33 versus K48 chain selection unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • How TRIM27 selects among its many substrates and chooses ubiquitin chain types (K48 versus atypical K6/K11/K33 versus non-catalytic outputs) across nuclear, endosomal, and mitochondrial pools remains unresolved.
  • No structural model of substrate recognition
  • Signals partitioning catalytic versus scaffolding/transcriptional functions undefined
  • Spatial regulation across compartments not integrated

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 6 GO:0016874 ligase activity 3 GO:0140096 catalytic activity, acting on a protein 3 GO:0003677 DNA binding 2 GO:0060090 molecular adaptor activity 2 GO:0140110 transcription regulator activity 2
Localization
GO:0005634 nucleus 3 GO:0005768 endosome 3 GO:0005739 mitochondrion 1
Pathway
R-HSA-162582 Signal Transduction 4 R-HSA-168256 Immune System 4 R-HSA-392499 Metabolism of proteins 4 R-HSA-9612973 Autophagy 3 R-HSA-5357801 Programmed Cell Death 2 R-HSA-4839726 Chromatin organization 1
Partners
CLEC16ANOD2PTENSTK38LTBK1ULK1USP7WNK1
Complex memberships
PRC1.1 (non-canonical Polycomb)TRIM27-USP7 complex

Evidence

Reading pass · 32 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1992 RFP (TRIM27) protein is strongly associated with the nuclear matrix and exhibits DNA-binding activity, preferentially binding double-stranded DNA over single-stranded DNA. Two forms were detected: a 58 kDa ubiquitous form and a 68 kDa testis-specific form. Western blot, nuclear matrix fractionation, DNA-binding assay Nucleic acids research Medium 1437549
1996 TRIM27 (RFP) homomultimerization occurs through its coiled-coil domain; the B-box domain is not a direct interaction interface but its structural integrity is necessary for coiled-coil-mediated multimerization. Mutations in the RING finger and B-box alter subcellular compartmentalization of RFP in cell lines. Yeast two-hybrid, deletion/mutation analysis, subcellular localization by microscopy Journal of cell science Medium 9247190
1996 The RING finger motif of RFP (TRIM27) is critical for the transforming activity of the RFP/RET fusion oncogene; mutation of Cys-16, Cys-31, or His-33 in the RING finger markedly decreased transforming activity and tyrosine phosphorylation, while mutations in the B-box (Cys-118 or His-124) had no effect. Site-directed mutagenesis, NIH 3T3 transformation assay, phosphorylation analysis Biochemical and biophysical research communications Medium 8753810
1996 Mouse rfp (TRIM27) protein is expressed as nuclear bodies resembling PML oncogenic domains (PODs) in cell nuclei, with highest expression in pachytene spermatocytes and round spermatids during spermatogenesis. Immunofluorescence, immunohistochemistry, subcellular localization in mouse tissues Developmental genetics Low 9023983
2002 RFP (TRIM27) interacts with Enhancer of Polycomb 1 (EPC1) via the EPcA, EPcB, and CT regions of EPC1. O-glycosylation of the 68 kDa testis form of RFP enhances interaction with the EPC1 CT region; expression of EPcA region impairs transcriptional repressive activity of RFP. GST pull-down, co-immunoprecipitation from mouse testis, O-glycosidase treatment, luciferase reporter assay Biochemical and biophysical research communications Medium 11779184
2005 RFP (TRIM27) interacts with bHLH transcription factors SCL, E47, MyoD, and mASH-1 (but not GATA-1 or PU.1) through its B-box and first coiled-coil region, and represses transcriptional activation by E47, MyoD, and mASH-1 in a trichostatin A-sensitive manner consistent with HDAC/Polycomb recruitment rather than ubiquitination-mediated degradation of the bHLH proteins. Co-immunoprecipitation, domain deletion mapping, luciferase reporter assay, trichostatin A treatment Oncogene Medium 16007160
2012 TRIM27 functions as an E3 ubiquitin ligase that physically interacts with NOD2 via the nucleotide-binding domain of NOD2; NOD2 activation enhances this interaction. TRIM27-dependent K48-linked ubiquitination of NOD2 leads to proteasomal degradation of NOD2, thereby negatively regulating NOD2-mediated pro-inflammatory signaling. Co-immunoprecipitation, ubiquitination assay, proteasome inhibitor experiments, siRNA knockdown with NF-κB reporter PloS one Medium 22829933
2012 TRIM27 negatively regulates FcεRI (IgE receptor) signaling in mast cells by ubiquitinating and inhibiting PI3KC2β; TRIM27-knockout mice show increased susceptibility to anaphylaxis in vivo. Co-immunoprecipitation, ubiquitination assay, KCa3.1 channel activation assay, TRIM27-/- mouse model, in vivo anaphylaxis model Molecular and cellular biology High 22645315
2013 TRIM27 forms a complex with USP7 (ubiquitin-specific protease), ubiquitinates USP7, and this complex controls TNF-α-induced apoptosis. USP7 in this complex deubiquitinates RIP1, which is required for TNF-α apoptotic signaling. Trim27-deficient MEFs and mice are resistant to TNF-α-induced apoptosis. Co-immunoprecipitation, ubiquitination assay, Trim27-/- mouse and MEF studies, TNF-α apoptosis assay Molecular and cellular biology High 24144979
2013 RFP (TRIM27) acts as a novel E3 ubiquitin ligase for PTEN, promoting atypical (non-K48) polyubiquitination of PTEN that does not affect PTEN stability or localization but inhibits PTEN phosphatase activity, thereby relieving PTEN-mediated inhibition of AKT activation. Co-immunoprecipitation, in-cell ubiquitination assay, RNAi knockdown, PTEN phosphatase activity assay, AKT activation assay Cell research Medium 23419514
2014 TRIM27 is a novel degradation target of the HSV-1 E3 ubiquitin ligase ICP0; TRIM27 protein is rapidly lost during HSV-1 infection in a manner dependent on ICP0's RING domain and the proteasome. A RING-mutant ICP0 co-immunoprecipitates endogenous TRIM27. Surprisingly, TRIM27 depletion decreases ICP0-null virus yields, suggesting TRIM27 can positively affect HSV-1 infection. Proteomic screen, co-immunoprecipitation, proteasome inhibitor treatment, RING-domain mutant ICP0, immunofluorescence, siRNA knockdown with viral yield assay Journal of virology Medium 25320289
2014 TRIM27 forms a complex with MRTF-B (myocardin-related transcription factor B) in leading cancer cells and posttranscriptionally upregulates integrin β1 expression via suppression of microRNA-124, enabling integrin β1 mRNA stability and translation specifically in leading cells of collectively invading cancer groups. Depletion of TRIM27 or MRTF-B abrogates integrin β1 upregulation and blocks collective cancer invasion. Co-immunoprecipitation, RNAi knockdown, invasion assay in vitro and in vivo, miRNA reporter assay Cell reports Medium 24794433
2015 TRIM27 is recruited by the Siglec1–DAP12–SHP2 signaling axis and induces K48-linked ubiquitination of TBK1 at Lys251 and Lys372, leading to TBK1 proteasomal degradation and suppression of type I IFN production during viral infection. Co-immunoprecipitation, ubiquitination assay with K48-linkage specific analysis, proteasome inhibitor, mutagenesis of TBK1 ubiquitination sites, RNAi knockdown Cell research High 26358190
2016 TRIM27 interacts with Slx2 and co-localizes with Slx2 in the XY body of spermatocytes during meiosis; Trim27 also co-localizes with γ-H2AX in the XY body, suggesting a role in XY body formation and meiotic regulation. Yeast two-hybrid, co-immunoprecipitation, mammalian two-hybrid, immunohistochemistry, immunocytochemistry Cell cycle Medium 27612028
2016 TRIM27 functions as a host restriction factor against Mycobacterium tuberculosis by promoting innate immune responses and cell apoptosis via the JNK/p38 MAPK pathway. Mtb effector PtpA antagonizes this function by competitively binding to TRIM27's RING domain. Co-immunoprecipitation, RNAi knockdown, macrophage infection assay, MAPK pathway analysis Scientific reports Medium 27698396
2018 TRIM27 localizes to retromer-positive punctate structures and serves as a scaffold recruiting gp130, JAK1, and STAT3 to these structures, facilitating STAT3 phosphorylation downstream of IL-6. Knockdown of TRIM27 impairs IL-6-induced STAT3 activation, inflammatory cytokine expression, and colitis-associated carcinogenesis in mouse models. Co-immunoprecipitation, subcellular fractionation/immunofluorescence, TRIM27 knockdown with STAT3 phosphorylation readout, DSS/AOM mouse colitis model Nature communications High 30143645
2018 USP7 physically interacts with TRIM27, stabilizing it; USP7 overexpression promotes TRIM27-dependent K48-linked ubiquitination and degradation of TBK1, thereby suppressing type I IFN signaling. USP7 knockout leads to enhanced TRIM27 degradation, reduced TBK1 ubiquitination, and enhanced IFN signaling. Co-immunoprecipitation, ubiquitination assay, USP7 knockout, overexpression with IFN reporter, viral replication assay FASEB journal Medium 29688809
2019 TRIM27 interacts with and promotes ubiquitination of PIAS3 (a STAT3 inhibitor) in keratinocytes, leading to PIAS3 degradation and enhanced IL-6-induced STAT3 activation, cell proliferation, and inflammation factor production. Co-immunoprecipitation, ubiquitination assay, TRIM27 overexpression/silencing, PIAS3 silencing, STAT3 phosphorylation readout American journal of physiology. Cell physiology Medium 31747314
2019 TRIM27 directly interacts with PTEN and mediates its poly-ubiquitination in esophageal squamous cell carcinoma cells, activating AKT signaling; AKT inhibitor LY294002 blocks TRIM27's pro-proliferative effects. Co-immunoprecipitation, ubiquitination assay, AKT inhibitor treatment, overexpression/knockdown Cancer cell international Medium 31719796
2020 TRIM27 mediates K48-linked ubiquitination and proteasomal degradation of SIX3 in non-small cell lung cancer cells, thereby activating β-catenin target gene expression (S100P, TGFB3, MMP-9); β-catenin inhibitor XAV939 blocks TRIM27-mediated cell proliferation and invasion. Co-immunoprecipitation, ubiquitination assay, siRNA knockdown, XAV939 inhibitor rescue, cell proliferation and invasion assay Aging Medium 33264103
2020 TRIM27 mediates ubiquitination and degradation of p21, thereby suppressing cellular senescence in non-triple-negative breast cancer cells. Co-immunoprecipitation (coIP), ubiquitination assay, TRIM27 knockdown/overexpression, senescence assay Molecular therapy. Nucleic acids Medium 33251042
2020 TRIM27 ubiquitinates and degrades PPARγ in neuronal HT22 cells, promoting glutamate-induced apoptosis and IL-1β release; PPARγ agonist rosiglitazone reverses TRIM27 overexpression-induced apoptosis. Co-immunoprecipitation, ubiquitination assay, TRIM27 knockdown/overexpression, rosiglitazone rescue, apoptosis assay Experimental cell research Medium 33385414
2021 TRIM27 and USP7 are integral components of the non-canonical PRC1.1 Polycomb complex. USP7 and TRIM27 are reciprocally required for incorporation into PRC1.1; USP7 counteracts TRIM27 E3 ligase activity to maintain PRC1.1 integrity. USP7 inhibition results in PRC1.1 disassembly, loss of chromatin binding, and reduced H2AK119ub and H3K27ac at PRC1.1 target loci. Co-immunoprecipitation/USP7 interactome analysis, ChIP, H2AK119ub and H3K27ac assays, USP7 inhibitor, TRIM27 knockdown, gene expression analysis iScience Medium 34113809
2021 TRIM27 directly interacts with IκBα (inhibitor of NF-κB) and promotes its K48-linked ubiquitination, leading to IκBα degradation and NF-κB activation in renal cell carcinoma cells. Co-immunoprecipitation, ubiquitination assay, NF-κB reporter, TRIM27 overexpression/siRNA knockdown BMC cancer Medium 34284744
2021 TRIM27 interacts with p53 and promotes its ubiquitination in cardiomyocytes; heart-conditional TRIM27 knockout exacerbates MI/R injury via enhanced p53 expression, caspase-3 activation, and NF-κB-mediated inflammation. Co-immunoprecipitation, ubiquitination assay, heart-conditional KO mouse, MI/R model, p53 inhibition rescue Biochemical and biophysical research communications Medium 33865220
2022 TRIM27 directly polyubiquitinates ULK1 at K568 and K571 with K48-linked chains (for degradation) and ubiquitinates STK38L with non-degradative K6/K11 chains; STK38L ubiquitination activates it to phosphorylate ULK1 at Ser495, making ULK1 a better substrate for TRIM27-mediated hyper-ubiquitination, thus restraining autophagy amplitude. TRIM27 knockout mice show increased basal autophagy. In vitro ubiquitination assay, site-directed mutagenesis at ubiquitination sites, kinase phosphorylation assay, TRIM27 KO mouse model, autophagy flux assay The EMBO journal High 35670107
2022 TRIM27 is an autophagy substrate degraded via ATG7/ATG9-dependent autophagy and interacts with autophagy receptor SQSTM1/p62; induced TRIM27 expression causes mitochondrial clustering facilitated by SQSTM1/p62, and promotes mitophagy by stabilizing phosphorylated TBK1 on mitochondria. TRIM27 KO cells show reduced mitophagy, rescued by EGFP-TRIM27 re-introduction. Co-immunoprecipitation, TRIM27 KO cells, mitophagy assay, immunofluorescence, ATG7/ATG9 dependency experiments, TBK1 inhibitor, EGFP-TRIM27 reconstitution The FEBS journal High 36111389
2022 CLEC16A localizes to early endosomes and interacts with endosomal retromer complex subunits and TRIM27; CLEC16A truncation abolishes both endosomal localization and interaction with TRIM27. CLEC16A knockdown increases TRIM27 adhesion to early endosomes and causes abnormal F-actin accumulation, indicating CLEC16A controls endosomal TRIM27 levels. Mass spectrometry interactome, co-immunoprecipitation, subcellular fractionation/imaging, domain truncation, CLEC16A knockdown with TRIM27 localization readout Human genetics Medium 36538041
2023 WNK1 binds TRIM27 and is required for the TRIM27-USP7 complex formation; WNK1 knockdown reduces TRIM27 protein levels, disrupts WASH complex ubiquitination and endosomal actin polymerization, and increases EGFR degradation after ligand stimulation. TRIM27 depletion phenocopies WNK1 depletion on EGFR trafficking. Co-immunoprecipitation, WNK1/TRIM27 siRNA knockdown, EGFR degradation assay, endosomal actin polymerization assay Proceedings of the National Academy of Sciences of the United States of America Medium 37307465
2023 TRIM27 stabilizes β-catenin and activates Wnt/β-catenin signaling to promote intestinal stem cell (ISC) self-renewal; Trim27 KO mice display IBS-like symptoms, and organoid formation is disrupted in Trim27-deficient intestinal tissue but rescued by β-catenin reintroduction. Trim27 KO mouse model, organoid formation assay, β-catenin rescue, Wnt/β-catenin pathway analysis Cellular & molecular immunology Medium 36596873
2024 Nuclear-localized TRIM27 acts as a transcriptional activator of TFEB (an autophagy master regulator) by binding to the TFEB promoter and interacting with the transcription factor CREB1, enhancing CREB1 binding to the TFEB promoter to promote autophagy-related gene expression and autophagy flux against Mycobacterium tuberculosis. This activity is E3 ligase-independent. ChIP-seq, EMSA (electrophoretic mobility shift assay), co-immunoprecipitation, TRIM27 KO cell and mouse model, autophagy flux assay, TFEB activator rescue Autophagy High 38390831
2024 TRIM27 ubiquitinates KLF12 at K326 via K33-linked polyubiquitination through its E3 ligase activity, reducing KLF12 transcriptional repression of L1CAM and thereby promoting cisplatin resistance and metastasis in esophageal squamous cell carcinoma. Co-immunoprecipitation, ubiquitination assay with K33-linkage specificity, domain mapping of interaction, siRNA knockdown, cisplatin resistance assay Drug resistance updates Medium 38924996

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 New rfp- and pES213-derived tools for analyzing symbiotic Vibrio fischeri reveal patterns of infection and lux expression in situ. Applied and environmental microbiology 226 16391121
2015 Siglec1 suppresses antiviral innate immune response by inducing TBK1 degradation via the ubiquitin ligase TRIM27. Cell research 161 26358190
2004 New Drosophila transgenic reporters: insulated P-element vectors expressing fast-maturing RFP. BioTechniques 156 15038159
2014 Mapping early fate determination in Lgr5+ crypt stem cells using a novel Ki67-RFP allele. The EMBO journal 130 25092767
2019 An Id2RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials. Immunity 128 30926235
1994 Two Mhc class I and two Mhc class II genes map to the chicken Rfp-Y system outside the B complex. Proceedings of the National Academy of Sciences of the United States of America 122 7910407
1996 Assignment of Rfp-Y to the chicken major histocompatibility complex/NOR microchromosome and evidence for high-frequency recombination associated with the nucleolar organizer region. Proceedings of the National Academy of Sciences of the United States of America 116 8632997
2012 TRIM27 negatively regulates NOD2 by ubiquitination and proteasomal degradation. PloS one 104 22829933
2013 Ubiquitination-deubiquitination by the TRIM27-USP7 complex regulates tumor necrosis factor alpha-induced apoptosis. Molecular and cellular biology 102 24144979
2018 Up-regulation of miR-383-5p suppresses proliferation and enhances chemosensitivity in ovarian cancer cells by targeting TRIM27. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 101 30399596
2018 Molecular Profiling of Salivary Gland Intraductal Carcinoma Revealed a Subset of Tumors Harboring NCOA4-RET and Novel TRIM27-RET Fusions: A Report of 17 cases. The American journal of surgical pathology 96 30045065
1997 Involvement of the rfp tripartite motif in protein-protein interactions and subcellular distribution. Journal of cell science 95 9247190
2019 NCOA4-RET and TRIM27-RET Are Characteristic Gene Fusions in Salivary Intraductal Carcinoma, Including Invasive and Metastatic Tumors: Is "Intraductal" Correct? The American journal of surgical pathology 94 31162284
1992 Role of Escherichia coli K-12 rfa genes and the rfp gene of Shigella dysenteriae 1 in generation of lipopolysaccharide core heterogeneity and attachment of O antigen. Journal of bacteriology 77 1385388
2018 TRIM27 mediates STAT3 activation at retromer-positive structures to promote colitis and colitis-associated carcinogenesis. Nature communications 72 30143645
2018 TRIM27 functions as an oncogene by activating epithelial-mesenchymal transition and p-AKT in colorectal cancer. International journal of oncology 67 29767249
2001 At least one class I gene in restriction fragment pattern-Y (Rfp-Y), the second MHC gene cluster in the chicken, is transcribed, polymorphic, and shows divergent specialization in antigen binding region. Journal of immunology (Baltimore, Md. : 1950) 65 11207288
2013 RFP-mediated ubiquitination of PTEN modulates its effect on AKT activation. Cell research 63 23419514
2002 Three novel human VMD2-like genes are members of the evolutionary highly conserved RFP-TM family. European journal of human genetics : EJHG 63 12032738
2016 The ubiquitin ligase TRIM27 functions as a host restriction factor antagonized by Mycobacterium tuberculosis PtpA during mycobacterial infection. Scientific reports 61 27698396
2009 Transthyretin mouse transgenes direct RFP expression or Cre-mediated recombination throughout the visceral endoderm. Genesis (New York, N.Y. : 2000) 61 19415627
2022 TRIM27 cooperates with STK38L to inhibit ULK1-mediated autophagy and promote tumorigenesis. The EMBO journal 56 35670107
1992 RFP is a DNA binding protein associated with the nuclear matrix. Nucleic acids research 56 1437549
2017 DNA mimics of red fluorescent proteins (RFP) based on G-quadruplex-confined synthetic RFP chromophores. Nucleic acids research 55 28981852
2004 Cloning and characterization of the murine Vmd2 RFP-TM gene family. Cytogenetic and genome research 53 15218265
2009 Simultaneous determination of labile proton concentration and exchange rate utilizing optimal RF power: Radio frequency power (RFP) dependence of chemical exchange saturation transfer (CEST) MRI. Journal of magnetic resonance (San Diego, Calif. : 1997) 52 19926319
2005 Localization and functional interrelationships among cytosolic Group IV, secreted Group V, and Ca2+-independent Group VI phospholipase A2s in P388D1 macrophages using GFP/RFP constructs. Biochimica et biophysica acta 52 15967714
2019 TRIM27 promotes IL-6-induced proliferation and inflammation factor production by activating STAT3 signaling in HaCaT cells. American journal of physiology. Cell physiology 50 31747314
2018 USP7-TRIM27 axis negatively modulates antiviral type I IFN signaling. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 50 29688809
2015 Downregulation of TRIM27 expression inhibits the proliferation of ovarian cancer cells in vitro and in vivo. Laboratory investigation; a journal of technical methods and pathology 47 26568293
2003 Novel tumorigenic rearrangement, Delta rfp/ret, in a papillary thyroid carcinoma from externally irradiated patient. Mutation research 47 12787916
1996 Association between the Rfp-Y haplotype and the incidence of Marek's disease in chickens. Immunogenetics 47 8753853
2019 TRIM27 promotes the development of esophagus cancer via regulating PTEN/AKT signaling pathway. Cancer cell international 46 31719796
2002 Improved version of the red fluorescent protein (drFP583/DsRed/RFP). BioTechniques 43 12238769
1996 Mapping of the genetically independent chicken major histocompatibility complexes B@ and RFP-Y@ to the same microchromosome by two-color fluorescent in situ hybridization. Cytogenetics and cell genetics 43 8995478
1986 Cloning of the rfb gene region of Shigella dysenteriae 1 and construction of an rfb-rfp gene cassette for the development of lipopolysaccharide-based live anti-dysentery vaccines. Microbial pathogenesis 43 2469931
1996 Mouse ret finger protein (rfp) proto-oncogene is expressed at specific stages of mouse spermatogenesis. Developmental genetics 42 9023983
2015 Single-molecule microscopy of molecules tagged with GFP or RFP derivatives in mammalian cells using nanobody binders. Methods (San Diego, Calif.) 41 26123185
2016 Two-step cultivation for production of astaxanthin in Chlorella zofingiensis using a patented energy-free rotating floating photobioreactor (RFP). Bioresource technology 39 27818161
2014 Identification of TRIM27 as a novel degradation target of herpes simplex virus 1 ICP0. Journal of virology 39 25320289
2008 Zebrafish primordial germ cell cultures derived from vasa::RFP transgenic embryos. Stem cells and development 39 18576915
2003 Analysis of part of the chicken Rfp-Y region reveals two novel lectin genes, the first complete genomic sequence of a class I alpha-chain gene, a truncated class II beta-chain gene, and a large CR1 repeat. Immunogenetics 39 12692693
2020 TRIM27 Functions as a Novel Oncogene in Non-Triple-Negative Breast Cancer by Blocking Cellular Senescence through p21 Ubiquitination. Molecular therapy. Nucleic acids 38 33251042
2015 Type I IFN-Inducible Downregulation of MicroRNA-27a Feedback Inhibits Antiviral Innate Response by Upregulating Siglec1/TRIM27. Journal of immunology (Baltimore, Md. : 1950) 38 26700765
2011 Role of IncP-1β plasmids pWDL7::rfp and pNB8c in chloroaniline catabolism as determined by genomic and functional analyses. Applied and environmental microbiology 37 22101050
2014 TRIM27/MRTF-B-dependent integrin β1 expression defines leading cells in cancer cell collectives. Cell reports 36 24794433
2006 Tumor cells genetically labeled with GFP in the nucleus and RFP in the cytoplasm for imaging cellular dynamics. Cell cycle (Georgetown, Tex.) 36 16760659
2021 The USP7-TRIM27 axis mediates non-canonical PRC1.1 function and is a druggable target in leukemia. iScience 35 34113809
1996 Histocompatibility antigen(s) linked to Rfp-Y (Mhc-like) genes in the chicken. Immunogenetics 35 8881037
1996 A RING finger motif regulates transforming activity of the rfp/ret fusion gene. Biochemical and biophysical research communications 34 8753810
2013 Distribution of MT1 melatonin receptor promoter-driven RFP expression in the brains of BAC C3H/HeN transgenic mice. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 33 24051358
2020 TRIM27 acts as an oncogene and regulates cell proliferation and metastasis in non-small cell lung cancer through SIX3-β-catenin signaling. Aging 31 33264103
1994 Lipopolysaccharide O-antigen biosynthesis in Shigella dysenteriae serotype 1: analysis of the plasmid-carried rfp determinant. Microbial pathogenesis 31 7520113
2012 Phosphatidylinositol-3-kinase C2β and TRIM27 function to positively and negatively regulate IgE receptor activation of mast cells. Molecular and cellular biology 30 22645315
2022 TRIM27-USP7 complex promotes tumour progression via STAT3 activation in human hepatocellular carcinoma. Liver international : official journal of the International Association for the Study of the Liver 29 35753056
2022 Emerging roles of TRIM27 in cancer and other human diseases. Frontiers in cell and developmental biology 29 36200036
2019 Intraductal Carcinoma of Salivary Glands Harboring TRIM27-RET Fusion with Mixed Low Grade and Apocrine Types. Head and neck pathology 28 30610524
2023 TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal. Cellular & molecular immunology 26 36596873
2019 Knockdown of TRIM27 expression suppresses the dysfunction of mesangial cells in lupus nephritis by FoxO1 pathway. Journal of cellular physiology 26 30648253
2015 Red fluorescent proteins (RFPs) and RFP-based biosensors for neuronal imaging applications. Neurophotonics 26 26158012
2021 TRIM27 interacts with Iκbα to promote the growth of human renal cancer cells through regulating the NF-κB pathway. BMC cancer 24 34284744
2012 Fine mapping and candidate gene analysis of the nuclear restorer gene Rfp for pol CMS in rapeseed (Brassica napus L.). TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 23 22614178
2020 TRIM27-mediated ubiquitination of PPARγ promotes glutamate-induced cell apoptosis and inflammation. Experimental cell research 22 33385414
2002 Generation of two-color transgenic zebrafish using the green and red fluorescent protein reporter genes gfp and rfp. Marine biotechnology (New York, N.Y.) 22 14961274
1997 Non-association between Rfp-Y major histocompatibility complex-like genes and susceptibility to Marek's disease virus-induced tumours in 6(3) x 7(2) F2 intercross chickens. Animal genetics 22 9363593
2024 TRIM27 elicits protective immunity against tuberculosis by activating TFEB-mediated autophagy flux. Autophagy 21 38390831
2023 TRIM27 ameliorates ischemic stroke by regulating NLRP3 inflammasome-mediated pyroptosis via the Akt/Nrf2/HO-1 signaling. Experimental neurology 21 37914066
2015 Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation. Biology of reproduction 21 26607717
2006 Characterization of the Interleukin (IL)-6 Inhibitor IL-6-RFP: fused receptor domains act as high affinity cytokine-binding proteins. The Journal of biological chemistry 21 17085445
2006 A keratin 18 transgenic zebrafish Tg(k18(2.9):RFP) treated with inorganic arsenite reveals visible overproliferation of epithelial cells. Toxicology letters 20 16376500
2006 Spatiotemporal expression of zebrafish keratin 18 during early embryogenesis and the establishment of a keratin 18:RFP transgenic line. Gene expression patterns : GEP 20 16446122
2021 TRIM27 contributes to glomerular endothelial cell injury in lupus nephritis by mediating the FoxO1 signaling pathway. Laboratory investigation; a journal of technical methods and pathology 19 33854173
2021 TRIM27 protects against cardiac ischemia-reperfusion injury by suppression of apoptosis and inflammation via negatively regulating p53. Biochemical and biophysical research communications 19 33865220
2021 Circ_0091579 exerts an oncogenic role in hepatocellular carcinoma via mediating miR-136-5p/TRIM27. Biomedical journal 19 34974169
2020 Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing. PloS one 19 31999769
2019 TRIM27 Promotes Hepatitis C Virus Replication by Suppressing Type I Interferon Response. Inflammation 19 30847745
2019 Characterization of Tg(Etv4-GFP) and Etv5 Reporter Lines in the Context of Fibroblast Growth Factor 10 Signaling During Mouse Embryonic Lung Development. Frontiers in genetics 18 30923534
2014 Deficiency of Trim27 protects dopaminergic neurons from apoptosis in the neurotoxin model of Parkinson's disease. Brain research 18 25223908
2003 Genotypic variability at the major histocompatibility complex (B and Rfp-Y) in Camperos broiler chickens. Animal genetics 18 12648091
2022 Subcellular localization of mutant P23H rhodopsin in an RFP fusion knock-in mouse model of retinitis pigmentosa. Disease models & mechanisms 17 35275162
2020 MicroRNA‑1 regulates the development of osteoarthritis in a Col2a1‑Cre‑ERT2/GFPfl/fl‑RFP‑miR‑1 mouse model of osteoarthritis through the downregulation of Indian hedgehog expression. International journal of molecular medicine 17 32626917
2016 Trim27 interacts with Slx2, is associated with meiotic processes during spermatogenesis. Cell cycle (Georgetown, Tex.) 17 27612028
2006 Common bile duct injection as a novel method for establishing red fluorescent protein (RFP)-expressing human pancreatic cancer in nude mice. JOP : Journal of the pancreas 17 16525203
2023 WNK1 controls endosomal trafficking through TRIM27-dependent regulation of actin assembly. Proceedings of the National Academy of Sciences of the United States of America 16 37307465
2022 TRIM27 suppresses inflammation injuries in pediatric pneumonia by targeting TLR4/NF-κB signaling pathway. Allergologia et immunopathologia 16 35257543
2022 TRIM27 is an autophagy substrate facilitating mitochondria clustering and mitophagy via phosphorylated TBK1. The FEBS journal 16 36111389
2020 Downregulation of TRIM27 suppresses gastric cancer cell proliferation via inhibition of the Hippo-BIRC5 pathway. Pathology, research and practice 16 32825933
2024 KLF12 interacts with TRIM27 to affect cisplatin resistance and cancer metastasis in esophageal squamous cell carcinoma by regulating L1CAM expression. Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 15 38924996
2016 Development and Validation of a Rex1-RFP Potency Activity Reporter Assay That Quantifies Stress-Forced Potency Loss in Mouse Embryonic Stem Cells. Stem cells and development 15 26651054
2008 Generation of neuronal-like cells from umbilical cord blood-derived mesenchymal stem cells of a RFP-transgenic cloned cat. The Journal of veterinary medical science 15 18685247
1997 New chicken Rfp-Y haplotypes on the basis of MHC class II RFLP and MLC analyses. Immunogenetics 15 9089091
2022 CLEC16A interacts with retromer and TRIM27, and its loss impairs endosomal trafficking and neurodevelopment. Human genetics 14 36538041
2015 Imaging the microenvironment of pancreatic cancer patient-derived orthotopic xenografts (PDOX) growing in transgenic nude mice expressing GFP, RFP, or CFP. Cancer letters 14 26742463
1998 Rfp-Y region polymorphism and Marek's disease resistance in multitrait immunocompetence-selected chicken lines. Poultry science 14 9565235
1987 Restriction fragment pattern (RFP) analysis of genomes from Danish isolates of suid herpesvirus 1 (Aujezsky's disease virus). Archives of virology 14 2827607
2022 Knockdown of TRIM27 alleviated sepsis-induced inflammation, apoptosis, and oxidative stress via suppressing ubiquitination of PPARγ and reducing NOX4 expression. Inflammation research : official journal of the European Histamine Research Society ... [et al.] 13 35962797
2002 Role for O-glycosylation of RFP in the interaction with enhancer of polycomb. Biochemical and biophysical research communications 13 11779184
2015 Duck TRIM27-L enhances MAVS signaling and is absent in chickens and turkeys. Molecular immunology 12 26254985
2013 Trim27-deficient mice are susceptible to streptozotocin-induced diabetes. FEBS open bio 11 24392305
2005 RFP represses transcriptional activation by bHLH transcription factors. Oncogene 11 16007160

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