Affinage

TNFAIP1

BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 · UniProt Q13829

Length
316 aa
Mass
36.2 kDa
Annotated
2026-06-10
55 papers in source corpus 20 papers cited in narrative 20 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

TNFAIP1 is a TNF-α-inducible protein that functions principally as a substrate adaptor for Cullin3-based RING E3 ubiquitin ligase complexes, directing K48-linked polyubiquitination and proteasomal degradation of target proteins (PMID:33553178, PMID:38102610). Through this activity it coordinates with Cullin3 to degrade the small GTPase RhoB (PMID:33553178), directly binds SNAP25 via its N-terminal residues (1–96) and ubiquitinates it at K69 (PMID:38102610), and promotes ubiquitin-mediated turnover of KCTD10 and CK2β (PMID:22810651, PMID:37302538). TNFAIP1 physically interacts with RhoB, and this interaction drives SAPK/JNK-mediated apoptosis; loss of TNFAIP1 stabilizes RhoB to induce pro-inflammatory IL-6/IL-8 production via p38/JNK MAPK signaling (PMID:19637314, PMID:33553178). In the nervous system TNFAIP1 binds the Rnd2/Rnd3 GTPases and controls cortical neuron positioning and dendritic maturation, and its degradation of SNAP25 modulates PINK1/Parkin mitophagy and caspase-3/GSDME pyroptosis in neurons (PMID:26969432, PMID:38102610); zebrafish knockout produces microcephaly, microphthalmia, and impaired neuronal gene expression (PMID:37239365). TNFAIP1 is itself a substrate of the CRL3^BTBD9 complex, which targets it for degradation, linking TNFAIP1 levels to cancer cell migration (PMID:32327643). Its transcription is driven basally by Sp1 and by ERβ in hippocampal cells, and it is negatively regulated by miR-93 (PMID:37302538, PMID:19593659, PMID:24737445). An early, distinct line of evidence identified the secreted/GPI-linked protein B61 (encoded by this locus) as a ligand that activates the ECK receptor tyrosine kinase and mediates TNF-α-induced angiogenesis (PMID:8139691, PMID:7890684, PMID:7536959).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1994 High

    Established the first molecular function for this locus by identifying its product B61 as an activating ligand for the ECK receptor tyrosine kinase, defining a receptor-ligand signaling axis.

    Evidence Receptor affinity chromatography, surface plasmon resonance, and cell-based ECK autophosphorylation assay

    PMID:8139691

    Open questions at the time
    • Did not connect B61/ECK signaling to the later-described intracellular adaptor/ubiquitin functions
    • Did not address whether secreted ligand and intracellular protein are the same physiological species
  2. 1995 High

    Showed B61 exists in both soluble and GPI-anchored cell-surface forms that both activate ECK, and that TNF-α drives angiogenesis via autocrine/paracrine B61-ECK signaling, embedding the protein in inflammatory vascular biology.

    Evidence PI-PLC treatment, B61-Ig chimera in vivo angiogenesis assay, endothelial chemotaxis, and antibody neutralization

    PMID:7536959 PMID:7890684

    Open questions at the time
    • Mechanism downstream of ECK activation not dissected
    • Relationship between this extracellular ligand role and intracellular functions unresolved
  3. 2009 High

    Reoriented understanding toward an intracellular role by identifying RhoB as a direct binding partner whose interaction with TNFAIP1 triggers SAPK/JNK-dependent apoptosis, and showed CK2 phosphorylates TNFAIP1 to control its nuclear localization and PCNA binding.

    Evidence Yeast two-hybrid, reciprocal Co-IP, in vitro binding, co-localization, siRNA rescue, in vitro/in vivo kinase assays, subcellular fractionation

    PMID:19637314 PMID:19851886

    Open questions at the time
    • Whether RhoB binding reflected adaptor/degradation activity not yet recognized
    • Functional consequence of PCNA interaction not defined
  4. 2009 Medium

    Defined basal transcriptional control by establishing a functional Sp1-binding site in the core promoter.

    Evidence Promoter deletion, EMSA, site-directed mutagenesis, ChIP, and reporter assay

    PMID:19593659

    Open questions at the time
    • Inducible/TNF-α-responsive elements not mapped
    • No link to upstream signaling that activates Sp1 at this promoter
  5. 2012 Medium

    Provided the first evidence that TNFAIP1 acts as an adaptor driving ubiquitin-mediated degradation, showing it binds KCTD10 and promotes its proteasomal turnover while both inhibit NF-κB and AP-1 activity.

    Evidence Yeast two-hybrid, GST pull-down, Co-IP, co-localization, MG132-reversible ubiquitination assay, reporter assays

    PMID:22810651

    Open questions at the time
    • E3 ligase complex mediating KCTD10 degradation not identified at this stage
    • Direct mechanism linking degradation to NF-κB/AP-1 suppression not shown
  6. 2016 Medium

    Extended TNFAIP1 function to neurodevelopment by identifying it as a Rnd2/Rnd3 GTPase partner required for cortical neuron positioning and dendritic maturation.

    Evidence In vitro interaction assay and in utero electroporation with immunofluorescence in postnatal mouse cortex

    PMID:26969432

    Open questions at the time
    • Whether neuronal phenotype reflects ubiquitin-adaptor activity on Rnd GTPases not established
    • Molecular substrate in this context not defined
  7. 2020 High

    Identified the upstream control of TNFAIP1 protein levels, showing the CRL3^BTBD9 complex polyubiquitinates and degrades TNFAIP1, linking BTBD9 loss to TNFAIP1-driven cancer cell migration.

    Evidence Label-free proteomics, reciprocal Co-IP, ubiquitination assay, migration assay, genetic rescue

    PMID:32327643

    Open questions at the time
    • Signals controlling CRL3^BTBD9 targeting of TNFAIP1 unknown
    • Degron on TNFAIP1 not mapped
  8. 2021 Medium

    Demonstrated that TNFAIP1 coordinates with Cullin3 to degrade RhoB, and that loss of TNFAIP1 stabilizes RhoB to drive p38/JNK-dependent IL-6/IL-8 induction, recasting the earlier RhoB interaction as a CRL3 substrate relationship.

    Evidence Co-IP, ubiquitin assay, cytokine ELISA, siRNA knockdown, pathway inhibitors in HCC cells

    PMID:33553178

    Open questions at the time
    • BTB/Cullin3 contact residues not mapped
    • Whether RhoB degradation occurs in non-cancer contexts not tested here
  9. 2023 High

    Provided the most direct biochemical definition of TNFAIP1 as a ubiquitin ligase adaptor by mapping its SNAP25-binding region (residues 1–96) and the K48 ubiquitination site (K69), linking SNAP25 degradation to neuronal mitophagy, pyroptosis, and postoperative cognitive dysfunction.

    Evidence Co-IP, K48-linkage-specific ubiquitination assay, domain mapping, AAV9-hSyn neuronal knockdown in mice, behavioral testing, SNAP25 co-depletion rescue

    PMID:38102610

    Open questions at the time
    • Cullin3 dependence in the SNAP25 context not directly confirmed
    • Generality of K48/K69 mechanism to other substrates not tested
  10. 2023 Medium

    Added CK2β as a TNFAIP1 ubiquitination substrate and defined miR-93 as a negative upstream regulator, linking TNFAIP1 to CK2/Akt/CREB signaling in DEHP neurotoxicity.

    Evidence Co-IP, ubiquitination assay, dual-luciferase reporter, miRNA overexpression in cells and mice

    PMID:37302538

    Open questions at the time
    • Reconciliation with earlier CK2β-as-kinase finding (idx 4) not addressed
    • E3 complex for CK2β degradation not specified
  11. 2023 Medium

    Established an organismal requirement for tnfaip1 in early neural development, showing knockout causes microcephaly, microphthalmia, and reduced neuronal marker expression.

    Evidence CRISPR/Cas9 knockout in zebrafish, in situ hybridization, qRT-PCR, transcriptome sequencing

    PMID:37239365

    Open questions at the time
    • Substrate(s) responsible for the developmental phenotype not identified
    • Cell-autonomous vs non-autonomous requirement unresolved
  12. 2024 Medium

    Broadened TNFAIP1's regulatory reach beyond protein degradation by showing it suppresses lncRNA LEENE/FoxO1/ABCA1-mediated lipid efflux, and is itself controlled transcriptionally via an EHMT2/HECTD2 axis.

    Evidence RNA immunoprecipitation, RNA pull-down, lentiviral knockdown, Oil Red O, HPLC, apoE-/- mice (idx 12); Co-IP, ChIP, qRT-PCR (idx 10)

    PMID:38688591 PMID:38878215

    Open questions at the time
    • Mechanism by which TNFAIP1 restrains LEENE expression unclear
    • Whether lipid and inflammation phenotypes share a common substrate not addressed
  13. 2026 Medium

    Identified PXR as a direct TNFAIP1 partner and showed TNFAIP1 positively regulates the PXR/CYP3A4 detoxification axis to inhibit HCC tumor growth.

    Evidence Co-IP, knockout, pharmacological PXR activation, qRT-PCR, in vivo xenograft

    PMID:42004057

    Open questions at the time
    • Whether PXR is a degradation substrate or stabilized partner not resolved
    • Mechanistic basis of positive regulation unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how the secreted/GPI-anchored B61-ECK ligand function relates to the intracellular CRL3 adaptor function of the same gene product, and which Cullin3 complex configuration operates on each substrate.
  • No study reconciles the extracellular ligand and intracellular ubiquitin-adaptor roles
  • BTB/Cullin3 binding interface and substrate-degron rules not systematically mapped
  • Substrate repertoire across tissues incompletely defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 4 GO:0048018 receptor ligand activity 3 GO:0060090 molecular adaptor activity 3 GO:0016874 ligase activity 1
Localization
GO:0005576 extracellular region 1 GO:0005634 nucleus 1 GO:0005768 endosome 1 GO:0005886 plasma membrane 1
Pathway
R-HSA-392499 Metabolism of proteins 3 R-HSA-5357801 Programmed Cell Death 3 R-HSA-1266738 Developmental Biology 2 R-HSA-162582 Signal Transduction 2
Partners
BTBD9CK2ΒKCTD10PXRRHOBRND2RND3SNAP25
Complex memberships
CRL3 (Cullin3-RING E3 ubiquitin ligase)

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1994 B61 (TNFAIP1) was identified as the ligand for the ECK receptor protein-tyrosine kinase; recombinant B61 induces autophosphorylation of ECK in intact cells, and B61 was purified by receptor affinity chromatography using the extracellular domain of ECK. Receptor affinity chromatography, surface plasmon resonance, cell-based autophosphorylation assay Nature High 8139691
1995 B61 can exist as a cell-surface glycosylphosphatidylinositol (GPI)-linked protein, in addition to its soluble secreted form, and the GPI-linked form is capable of activating the ECK receptor protein-tyrosine kinase. PI-PLC treatment, cell-based receptor activation assay, biochemical characterization The Journal of biological chemistry High 7890684
1995 B61, acting through the ECK receptor tyrosine kinase, functions as an angiogenic factor in vivo and as a chemoattractant for endothelial cells in vitro; TNF-alpha induces angiogenesis through induction of B61, which then activates ECK in an autocrine/paracrine loop, and an anti-B61 antibody attenuated TNF-alpha-induced but not bFGF-induced angiogenesis. B61-immunoglobulin chimera in vivo angiogenesis assay, endothelial cell chemotaxis assay, antibody neutralization Science High 7536959
2009 TNFAIP1 directly interacts with RhoB; co-immunoprecipitation and in vitro binding assays confirmed the interaction. RFP-TNFAIP1 partially co-localizes with EGFP-RhoB in endosomes. TNFAIP1 elicits pro-apoptotic activity, and simultaneous expression of RhoB and TNFAIP1 dramatically increases apoptosis in HeLa cells via SAPK/JNK signaling; knockdown of RhoB by siRNA rescued cells from TNFAIP1-induced apoptosis. Yeast two-hybrid, co-immunoprecipitation, in vitro binding assay, fluorescence co-localization, siRNA knockdown, JNK inhibitor experiment International journal of cancer High 19637314
2009 CK2 (casein kinase 2) phosphorylates TNFAIP1 both in vitro and in vivo; CK2β was identified as a TNFAIP1-interacting partner. Phosphorylation by CK2 facilitates nuclear distribution of TNFAIP1 and enhances its interaction with PCNA. Yeast two-hybrid screening, in vitro kinase assay, in vivo phosphorylation assay, subcellular fractionation, co-immunoprecipitation Molecular biology reports Medium 19851886
2012 TNFAIP1 interacts with KCTD10, confirmed by yeast two-hybrid, GST pull-down, co-immunoprecipitation, and co-localization. TNFAIP1 overexpression promotes ubiquitin-mediated proteasomal degradation of KCTD10 (reversed by MG132), and both TNFAIP1 and KCTD10 inhibit NF-κB and AP-1 transcriptional activity. Yeast two-hybrid, GST pull-down, co-immunoprecipitation, co-localization, ubiquitin/proteasome assay, reporter assay Molecular biology reports Medium 22810651
2016 TNFAIP1 (Bacurd2) acts as an interacting partner to Rnd2 and Rnd3 GTPases in vitro. In utero electroporation experiments in mice show that disruption of Tnfaip1/Bacurd2 expression impairs the long-term positioning of cortical neurons within the postnatal cerebral cortex and alters branching and dendritic spine properties of layer II/III projection neurons. In vitro interaction assay, in utero electroporation, immunofluorescence in postnatal mouse brain Neural development Medium 26969432
2020 The CRL3BTBD9 E3 ubiquitin ligase complex targets TNFAIP1 for polyubiquitination and proteasomal degradation: Cul3-ROC1 (CRL3) specifically interacts with TNFAIP1 and promotes its degradation, with BTBD9 as the specific substrate adaptor. Downregulation of BTBD9 promotes lung cancer cell migration by upregulating TNFAIP1, and TNFAIP1 deletion abrogated this effect. Label-free quantitative proteomics, co-immunoprecipitation, ubiquitination assay, cell migration assay, genetic rescue experiment Signal transduction and targeted therapy High 32327643
2021 TNFAIP1 coordinates with Cullin3 to mediate RhoB degradation through the ubiquitin proteasome system in hepatocellular carcinoma cells. Downregulation of TNFAIP1 induces expression of pro-inflammatory cytokines IL-6 and IL-8 in TNFα-stimulated cells through p38/JNK MAPK pathway via blocking RhoB degradation. Co-immunoprecipitation, ubiquitin assay, cytokine ELISA, siRNA knockdown, pathway inhibitor experiments Frontiers in cell and developmental biology Medium 33553178
2023 TNFAIP1 acts as an E3 ubiquitin ligase that directly binds SNAP25 (via its N-terminal residues 1-96) and mediates K48-linked polyubiquitination of SNAP25 at K69, leading to proteasomal degradation of SNAP25. Neuron-specific knockdown of TNFAIP1 in mice ameliorated postoperative cognitive dysfunction, restored PINK1/Parkin mitophagy, and reduced caspase-3/GSDME pyroptosis; these effects were reversed by SNAP25 co-depletion. Co-immunoprecipitation, ubiquitination assay with K48-linkage-specific analysis, domain-mapping, AAV9-hSyn neuronal knockdown in mice, behavioral testing, western blot Cell communication and signaling : CCS High 38102610
2024 HECTD2 promotes ubiquitin-mediated degradation of EHMT2, leading to upregulation of TNFAIP1 transcription (ChIP confirmed TNFAIP1 as a direct target of EHMT2). High TNFAIP1 expression promotes inflammatory response in renal cell carcinoma cells via the p38/JNK pathway; p38/JNK inhibitors attenuated the effect of TNFAIP1 overexpression. Co-immunoprecipitation, western blot, ChIP assay, qRT-PCR, ELISA, pathway inhibitor experiments In vivo (Athens, Greece) Medium 38688591
2023 TNFAIP1 interacts with CK2β and promotes its degradation by ubiquitination (confirmed by co-immunoprecipitation and western blotting); miR-93 negatively regulates TNFAIP1 expression (dual-luciferase reporter), and miR-93 overexpression prevented DEHP-induced neurotoxicity by downregulating TNFAIP1 and then activating CK2/Akt/CREB pathway. Co-immunoprecipitation, western blot, ubiquitination assay, dual-luciferase reporter assay, miRNA overexpression in cells and mice Food and chemical toxicology Medium 37302538
2024 Knockdown of TNFAIP1 in THP-1 macrophages enhanced lncRNA LEENE expression, promoted direct interaction of LEENE with FoxO1 protein (verified by RNA immunoprecipitation and RNA pull-down), stimulated FoxO1 proteasomal degradation, induced ABCA1 transcription, and suppressed lipid accumulation; in apoE-/- mice, TNFAIP1 knockdown upregulated ABCA1, improved lipid profiles, and attenuated atherosclerotic lesion area. Lentiviral knockdown, RNA immunoprecipitation, RNA pull-down, western blot, qRT-PCR, Oil Red O staining, HPLC, in vivo mouse model Journal of physiology and biochemistry Medium 38878215
2009 The TNFAIP1 gene promoter contains a functional Sp1-binding site within the core promoter region; gel EMSA, site-directed mutagenesis, and ChIP confirmed Sp1 binding in vivo and in vitro, and Sp1 overexpression enhanced TNFAIP1 promoter activity. Deletion mutation analysis, gel electrophoretic mobility shift assay (EMSA), site-directed mutagenesis, chromatin immunoprecipitation (ChIP), reporter assay Molecular biology reports Medium 19593659
2014 Estrogen receptor β (ERβ) binds to the Tnfaip1 promoter and upregulates Tnfaip1 expression in mouse hippocampal cells; ovariectomy increased Tnfaip1 expression in hippocampus, and primary hippocampal cell culture experiments with estrogen and ER antagonists confirmed regulation of Tnfaip1 levels by estrogen. Reporter assay, promoter binding site identification, in vivo ovariectomy model, primary hippocampal cell culture, immunostaining International journal of molecular medicine Medium 24737445
2023 In zebrafish, CRISPR/Cas9-mediated knockout of tnfaip1 caused developmental delays, microcephaly and microphthalmia, and decreased expression of neuronal marker genes (tuba1b, neurod1, ccnd1), establishing a role for tnfaip1 in early embryonic neural development. CRISPR/Cas9 knockout in zebrafish, whole mount in situ hybridization, qRT-PCR, transcriptome sequencing Genes Medium 37239365
2026 TNFAIP1 directly interacts with PXR (pregnane X receptor), confirmed by co-immunoprecipitation. TNFAIP1 positively regulates the PXR/CYP3A4 pathway; TNFAIP1 knockout decreased CYP3A4 expression and impaired PXR agonist (rifampicin)-induced CYP3A4 upregulation in HCC cells. In vivo, Tnfaip1 overexpression upregulated Pxr/Cyp3a11 and inhibited tumor growth. Co-immunoprecipitation, western blot, qRT-PCR, TNFAIP1 knockout, pharmacological PXR activation, in vivo tumor xenograft American journal of cancer research Medium 42004057
2016 TNFAIP1 knockdown inhibits growth and induces apoptosis in osteosarcoma cells, associated with downregulation of p65 NF-κB, PCNA, and MMP-2 and upregulation of caspase-3, placing TNFAIP1 upstream of NF-κB in osteosarcoma cell survival signaling. Lentiviral siRNA knockdown, MTT assay, Transwell invasion assay, flow cytometry, western blot for NF-κB/PCNA/MMP-2/caspase-3 Oncology reports Low 24969828
2020 TNFAIP1 overexpression inhibits levels of phosphorylated Akt and CREB and anti-apoptotic Bcl-2, contributing to Aβ-induced neurotoxicity; TNFAIP1 knockdown reversed these effects and reduced cleaved caspase-3, placing TNFAIP1 upstream of the Akt/CREB/Bcl-2 apoptotic axis. siRNA knockdown, overexpression, western blot for p-Akt, p-CREB, Bcl-2, cleaved caspase-3 in N2a cells BMC neuroscience Low 27430312
2020 TNFAIP1 is upregulated in APP/PS1 mouse neurons; Aβ increases binding of TNFAIP1 to RhoB (co-immunoprecipitation), and RhoB knockdown attenuates TNFAIP1-induced apoptosis in SH-SY5Y cells, confirming that TNFAIP1-RhoB interaction mediates apoptosis in an AD model. Co-immunoprecipitation, siRNA knockdown, western blot, flow cytometry, APP/PS1 transgenic mice immunostaining Journal of molecular neuroscience Medium 33159672

Source papers

Stage 0 corpus · 55 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1995 Role of B61, the ligand for the Eck receptor tyrosine kinase, in TNF-alpha-induced angiogenesis. Science (New York, N.Y.) 340 7536959
1994 B61 is a ligand for the ECK receptor protein-tyrosine kinase. Nature 224 8139691
2023 Pembrolizumab plus lenvatinib as first-line therapy for advanced non-clear-cell renal cell carcinoma (KEYNOTE-B61): a single-arm, multicentre, phase 2 trial. The Lancet. Oncology 130 37451291
1995 Protein B61 as a new growth factor: expression of B61 and up-regulation of its receptor epithelial cell kinase during melanoma progression. Cancer research 104 7780963
2000 Identification of dominant optimal HLA-B60- and HLA-B61-restricted cytotoxic T-lymphocyte (CTL) epitopes: rapid characterization of CTL responses by enzyme-linked immunospot assay. Journal of virology 89 10954555
2004 HLA-B60 and B61 are strongly associated with ankylosing spondylitis in HLA-B27-negative Taiwan Chinese patients. Rheumatology (Oxford, England) 81 15113995
1997 Epithelial cell kinase-B61: an autocrine loop modulating intestinal epithelial migration and barrier function. The American journal of physiology 74 9357823
2009 RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. International journal of cancer 66 19637314
1995 Characterization of B61, the ligand for the Eck receptor protein-tyrosine kinase. The Journal of biological chemistry 55 7890684
2017 Effects of XIST/miR-137 axis on neuropathic pain by targeting TNFAIP1 in a rat model. Journal of cellular physiology 49 29115665
2017 miR-137 attenuates Aβ-induced neurotoxicity through inactivation of NF-κB pathway by targeting TNFAIP1 in Neuro2a cells. Biochemical and biophysical research communications 47 28655611
2012 microRNA-372 maintains oncogene characteristics by targeting TNFAIP1 and affects NFκB signaling in human gastric carcinoma cells. International journal of oncology 41 23242208
2015 MiRNA-15a inhibits proliferation, migration and invasion by targeting TNFAIP1 in human osteosarcoma cells. International journal of clinical and experimental pathology 37 26261520
1996 B61, a ligand for the Eck receptor protein-tyrosine kinase, exhibits neurotrophic activity in cultures of rat spinal cord neurons. Journal of neuroscience research 35 8984203
2010 Complex sense-antisense architecture of TNFAIP1/POLDIP2 on 17q11.2 represents a novel transcriptional structural-functional gene module involved in breast cancer progression. BMC genomics 34 20158880
2013 MicroRNA-373 is upregulated and targets TNFAIP1 in human gastric cancer, contributing to tumorigenesis. Oncology letters 33 24179536
2009 CK2 phosphorylates TNFAIP1 to affect its subcellular localization and interaction with PCNA. Molecular biology reports 33 19851886
2017 MiR-424 Promotes Non-Small Cell Lung Cancer Progression and Metastasis through Regulating the Tumor Suppressor Gene TNFAIP1. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 32 28535539
2023 Neuronal-specific TNFAIP1 ablation attenuates postoperative cognitive dysfunction via targeting SNAP25 for K48-linked ubiquitination. Cell communication and signaling : CCS 29 38102610
2020 Circ0001320 inhibits lung cancer cell growth and invasion by regulating TNFAIP1 and TPM1 expression through sponging miR-558. Human cell 27 33128699
2016 Bacurd1/Kctd13 and Bacurd2/Tnfaip1 are interacting partners to Rnd proteins which influence the long-term positioning and dendritic maturation of cerebral cortical neurons. Neural development 27 26969432
2014 Knockdown of TNFAIP1 inhibits growth and induces apoptosis in osteosarcoma cells through inhibition of the nuclear factor-κB pathway. Oncology reports 27 24969828
2019 Knockdown of TNFAIP1 prevents di-(2-ethylhexyl) phthalate-induced neurotoxicity by activating CREB pathway. Chemosphere 26 31683445
2016 TNFAIP1 contributes to the neurotoxicity induced by Aβ25-35 in Neuro2a cells. BMC neuroscience 25 27430312
2016 Caudatin targets TNFAIP1/NF-κB and cytochrome c/caspase signaling to suppress tumor progression in human uterine cancer. International journal of oncology 25 27633631
2021 Inhibition of TNFAIP1 ameliorates the oxidative stress and inflammatory injury in myocardial ischemia/reperfusion injury through modulation of Akt/GSK-3β/Nrf2 pathway. International immunopharmacology 19 34330059
2020 The CRL3BTBD9 E3 ubiquitin ligase complex targets TNFAIP1 for degradation to suppress cancer cell migration. Signal transduction and targeted therapy 19 32327643
2015 Interaction between microRNA-181a and TNFAIP1 regulates pancreatic cancer proliferation and migration. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 19 26152285
2012 TNFAIP1 interacts with KCTD10 to promote the degradation of KCTD10 proteins and inhibit the transcriptional activities of NF-κB and AP-1. Molecular biology reports 18 22810651
2022 LncRNA KCNQ1OT1 knockdown inhibits ox-LDL-induced inflammatory response and oxidative stress in THP-1 macrophages through the miR-137/TNFAIP1 axis. Cytokine 16 35598525
2020 TNFAIP1 Mediates Formaldehyde-Induced Neurotoxicity by Inhibiting the Akt/CREB Pathway in N2a Cells. Neurotoxicity research 16 32335808
2020 miR-29c-3p Increases Cell Viability and Suppresses Apoptosis by Regulating the TNFAIP1/NF-κB Signaling Pathway via TNFAIP1 in Aβ-Treated Neuroblastoma Cells. Neurochemical research 16 32712875
1995 Molecular cloning and expression of rat and mouse B61 gene: implications on organogenesis. Oncogene 16 7675446
2009 Functional characterization of the promoter region of human TNFAIP1 gene. Molecular biology reports 15 19593659
2021 LncRNA p21, downregulating miR-181b, aggravates neuropathic pain by upregulating Tnfaip1 and inhibit the AKT/CREB axis. Brain research bulletin 14 33774143
2020 TNFAIP1 Is Upregulated in APP/PS1 Mice and Promotes Apoptosis in SH-SY5Y Cells by Binding to RhoB. Journal of molecular neuroscience : MN 14 33159672
1998 Diversity of HLA-B61 alleles and haplotypes in East Asians and Spanish Gypsies. Tissue antigens 14 9583807
2023 MiR-93 alleviates DEHP plasticizer-induced neurotoxicity by negatively regulating TNFAIP1 and inhibiting ubiquitin-mediated degradation of CK2β. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 12 37302538
2023 LncRNA NKILA Exacerbates Alzheimer's Disease Progression by Regulating the FOXA1-Mediated Transcription of TNFAIP1. Neurochemical research 11 37217807
2022 Epigenetic axis of SNHG19/miR-137/TNFAIP1 modulates amyloid beta peptide 25-35-induced SH-SY5Y cytotoxicity. Epigenomics 11 35170354
2022 Downregulation of TNFAIP1 alleviates OGD/R‑induced neuronal damage by suppressing Nrf2/GPX4‑mediated ferroptosis. Experimental and therapeutic medicine 10 36561622
2025 First-line Pembrolizumab Plus Lenvatinib for Advanced Non-clear-cell Renal Cell Carcinoma: Updated Results from the Phase 2 KEYNOTE-B61 Trial. European urology 8 40707309
2021 Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB. Frontiers in cell and developmental biology 8 33553178
2014 Estrogen is a novel regulator of Tnfaip1 in mouse hippocampus. International journal of molecular medicine 8 24737445
1997 Identification of Elf-1 and B61 as high affinity ligands for the receptor tyrosine kinase MDK1. Oncogene 7 9010230
2023 Knocking down TNFAIP1 alleviates inflammation and oxidative stress in pediatric pneumonia through PI3K/Akt/Nrf2 pathway. Allergologia et immunopathologia 5 37422785
2023 Biochemical Characterization of Novel GH6 Endoglucanase from Myxococcus sp. B6-1 and Its Effects on Agricultural Straws Saccharification. Foods (Basel, Switzerland) 5 37444255
2002 HLA-B*3531, a hybrid of B35 and B61, implications for diagnostic approaches to alleles with complex ancestral compositions. Tissue antigens 5 12366790
2023 CRISPR/Cas9-Mediated Knockout of tnfaip1 in Zebrafish Plays a Role in Early Development. Genes 4 37239365
2024 TNFAIP1 promotes macrophage lipid accumulation and accelerates the development of atherosclerosis through the LEENE/FoxO1/ABCA1 pathway. Journal of physiology and biochemistry 3 38878215
1998 Structure and chromosomal mapping of the TNF-alpha inducible endothelial protein 1 (Edp1) gene in the mouse. Biochimica et biophysica acta 3 9804998
2024 HECTD2/TNFAIP1 Axis Regulating the p38/JNK Pathway to Promote an Inflammatory Response in Renal Cell Carcinoma Cells. In vivo (Athens, Greece) 2 38688591
2023 Screening of Tnfaip1-Interacting Proteins in Zebrafish Embryonic cDNA Libraries Using a Yeast Two-Hybrid System. Current issues in molecular biology 1 37886961
2013 Identification of a new allele polymorphism (HLA-B*40:79) and correlation with the HLA-B40 (B60 and B61) antigens. Tissue antigens 1 24461007
2026 TNFAIP1 suppresses hepatocellular carcinoma progression via the PXR/CYP3A4 signaling axis. American journal of cancer research 0 42004057

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