Affinage

TMEM132A

Transmembrane protein 132A · UniProt Q24JP5

Round 2 corrected
Length
1023 aa
Mass
110.1 kDa
Annotated
2026-04-28
107 papers in source corpus 10 papers cited in narrative 10 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

TMEM132A is a single-pass type I transmembrane protein that functions as a positive regulator of both canonical Wnt/β-catenin and planar cell polarity (PCP) signaling during embryonic development. It physically interacts with the Wnt transporter WLS to stabilize Wnt ligand and enhance WLS–Wnt interaction (PMID:33324648), and with the co-receptor LRP6 to prevent its lysosomal degradation, thereby sustaining canonical Wnt signaling (PMID:39385148); it also binds the PCP components CELSR1 and FZD6, acting synergistically with Vangl2 to regulate neural tube closure (PMID:37390294). Loss of Tmem132a in mice causes spina bifida, caudal truncation, and hindgut/genitourinary malformations, with underlying defects in integrin-mediated mesodermal cell migration and visceral endoderm displacement (PMID:35950911, PMID:37390294). TMEM132A is enriched in the CNS, where it localizes to ER–Golgi compartments and the cell surface, and has been identified as a target autoantigen in autoimmune cerebellar ataxia (PMID:12514190, PMID:41564469).

Mechanistic history

Synthesis pass · year-by-year structured walk · 8 steps
  1. 2003 Medium

    The initial cloning of the rat ortholog established TMEM132A as an ER–Golgi-resident transmembrane protein that binds the chaperone GRP78 and confers resistance to serum-deprivation-induced cell death, providing the first molecular handle on the protein.

    Evidence GST pull-down, GFP/Myc-tagged overexpression with ER–Golgi colocalization, cell viability assay in Neuro2a cells

    PMID:12514190

    Open questions at the time
    • GRP78 interaction not confirmed by reciprocal approach
    • mechanism linking GRP78 binding to cell survival undefined
    • relevance of ER–Golgi localization to in vivo function unknown
  2. 2006 Medium

    Gain- and loss-of-function experiments revealed that TMEM132A negatively regulates STAT3 phosphorylation downstream of cAMP, suppressing GFAP expression in glioblastoma cells — the first signaling pathway placement for the protein.

    Evidence Stable overexpression and siRNA knockdown in C6 glioblastoma cells, phospho-STAT3 Western blot, GFAP qPCR

    PMID:16806201

    Open questions at the time
    • Mechanism of STAT3 regulation not identified
    • Not validated in non-cancer or primary neural cells
    • Relationship to Wnt/PCP functions discovered later remains unclear
  3. 2020 Medium

    Identification of TMEM132A as a binding partner of WLS that stabilizes Wnt ligand and enhances WLS–Wnt interaction placed TMEM132A directly in the canonical Wnt signaling pathway for the first time.

    Evidence Co-immunoprecipitation, Wnt ligand stabilization assay, TCF/LEF luciferase reporter in overexpression and knockdown studies

    PMID:33324648

    Open questions at the time
    • Which Wnt ligands are affected was not specified
    • In vivo relevance of WLS interaction not yet demonstrated at the time
    • Domain mapping of TMEM132A–WLS interaction not performed
  4. 2022 High

    Tmem132a-null mice exhibited spina bifida and caudal truncation due to impaired integrin-mediated lateral migration of caudal paraxial mesoderm, establishing the first in vivo developmental requirement and revealing a distinct integrin-regulatory mechanism.

    Evidence Tmem132a knockout mouse, neural tube phenotype analysis, cell migration assays, integrin expression and activation analysis

    PMID:35950911

    Open questions at the time
    • Direct physical interaction with integrins not demonstrated
    • Whether integrin regulation is Wnt-dependent or an independent function was unresolved
    • Rescue experiments with integrin pathway restoration not reported
  5. 2023 High

    Physical interactions with CELSR1 and FZD6 and genetic epistasis with Vangl2 identified TMEM132A as a new component of the planar cell polarity pathway, explaining hindgut and genitourinary defects in mutant mice through failure of visceral endoderm displacement.

    Evidence Co-immunoprecipitation of TMEM132A with CELSR1 and FZD6, Tmem132a;Vangl2 double-mutant epistasis analysis, histological phenotyping

    PMID:37390294

    Open questions at the time
    • Whether TMEM132A is a core or tissue-specific PCP component is unclear
    • Structural basis of CELSR1/FZD6 interaction unknown
    • Relative contributions of PCP vs. canonical Wnt to each developmental phenotype not fully delineated
  6. 2024 High

    Demonstration that TMEM132A binds LRP6 and prevents its lysosomal degradation unified the earlier WLS interaction with a receptor-level mechanism, showing TMEM132A amplifies canonical Wnt signaling at two nodes — ligand production and receptor stability.

    Evidence Co-immunoprecipitation of TMEM132A with LRP6, lysosomal inhibition rescue, Tmem132a knockout mouse phenotype, Wnt reporter assays in knockdown cells

    PMID:39385148

    Open questions at the time
    • Whether TMEM132A acts in a ternary complex with WLS and LRP6 simultaneously is untested
    • Structural determinants of LRP6 protection from lysosomal sorting unknown
    • Relative importance of WLS-stabilization vs. LRP6-stabilization in vivo not dissected
  7. 2024 Medium

    E2F1 was shown to directly bind the TMEM132A promoter and activate its transcription, providing the first upstream transcriptional regulatory mechanism for TMEM132A expression in a cancer context.

    Evidence Dual-luciferase reporter assay, siRNA knockdown of E2F1 and TMEM132A, CCK-8 proliferation assay in prostate cancer cells

    PMID:39473405

    Open questions at the time
    • Whether E2F1 regulates TMEM132A in normal developmental contexts is unknown
    • Chromatin immunoprecipitation confirmation of promoter occupancy not shown
    • Downstream effectors (Wnt, integrin, PCP) not assessed in this cancer model
  8. 2026 Medium

    Identification of TMEM132A as a target autoantigen in autoimmune cerebellar ataxia confirmed its CNS enrichment and surface accessibility, extending its relevance to neurological autoimmune disease.

    Evidence Immunoprecipitation/mass spectrometry antigen identification, cell-based binding assay validating patient IgG, immunohistochemistry in CNS tissue

    PMID:41564469

    Open questions at the time
    • Pathogenic role of anti-TMEM132A antibodies not demonstrated
    • Small patient cohort limits generalizability
    • Which TMEM132A extracellular domain epitope is targeted is undefined

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include whether TMEM132A's Wnt, PCP, and integrin functions represent a single integrated mechanism or separable activities, the structural basis for its interactions with WLS/LRP6/CELSR1/FZD6, and whether its cell-adhesion domain architecture contributes to any of its known signaling roles.
  • No structural model of TMEM132A or its complexes exists
  • Domain-deletion mapping for separating Wnt vs. PCP vs. integrin functions has not been performed
  • Predicted cohesin and immunoglobulin domains lack experimental functional validation

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098631 cell adhesion mediator activity 2 GO:0098772 molecular function regulator activity 2
Localization
GO:0005783 endoplasmic reticulum 1 GO:0005794 Golgi apparatus 1 GO:0005886 plasma membrane 1
Pathway
R-HSA-162582 Signal Transduction 4 R-HSA-1266738 Developmental Biology 3
Partners
CELSR1FZD6HSPA5LRP6WLS

Evidence

Reading pass · 10 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 A rat brain protein of 1021 amino acids with two predicted transmembrane regions, subsequently identified as the rat ortholog of TMEM132A, was cloned and shown to localize to the endoplasmic reticulum–Golgi domain. A GST pull-down assay identified GRP78 (a heat shock protein/chaperone) as a direct binding partner. Overexpression in Neuro2a cells conferred resistance to serum deprivation-induced cell death without affecting cell growth. PCR-selected cDNA subtraction, GST pull-down assay, GFP/Myc-tagged overexpression with fluorescence microscopy (ER-Golgi colocalization), cell viability assay under serum deprivation The Journal of biological chemistry Medium 12514190
2006 Stable overexpression of the rat TMEM132A ortholog (GBP) in C6 glioblastoma cells attenuated cAMP-induced GFAP expression and decreased cAMP-induced STAT3 phosphorylation. Conversely, siRNA-mediated knockdown of the endogenous protein markedly augmented cAMP-induced GFAP mRNA expression and hyper-phosphorylation of STAT3, demonstrating that TMEM132A negatively regulates STAT3 phosphorylation downstream of cAMP signaling. Stable overexpression, siRNA knockdown, Western blot for phospho-STAT3, RT-PCR/qPCR for GFAP mRNA FEBS letters Medium 16806201
2018 Bioinformatic sequence and structural analysis revealed that the TMEM132 family proteins (including TMEM132A) possess an extracellular domain architecture comprising three tandem immunoglobulin domains and a cohesin domain homologue—the first cohesin domain identified in animals. This architecture, with predicted intracellular connection to the actin cytoskeleton, strongly indicates a cell adhesion function. The architecture has an ancient holozoan origin predating the emergence of choanoflagellates and metazoa. In-depth sequence analysis, structural homology modeling, phylogenetic analysis Bioinformatics (Oxford, England) Low 29088312
2020 TMEM132A was identified as a novel regulator of the Wnt signaling pathway through its physical and functional interaction with Wntless (WLS), the Wnt ligand transporter. Co-immunoprecipitation demonstrated a direct TMEM132A–WLS interaction. TMEM132A stabilizes Wnt ligand, enhances WLS–Wnt ligand interaction, and activates the Wnt signaling pathway in signal-sending cells. Co-immunoprecipitation, Wnt ligand stabilization assay, Wnt pathway reporter assay (TCF/LEF luciferase), overexpression and knockdown studies Frontiers in cell and developmental biology Medium 33324648
2022 TMEM132A is required for caudal neural tube closure and lateral migration of caudal paraxial mesoderm in mice. Tmem132a-null mouse embryos and TMEM132A-depleted cell models showed dysregulation of multiple integrins and downstream integrin pathway signaling, as well as impaired cell migration behaviors, demonstrating that TMEM132A regulates integrin-based mesodermal cell migration essential for posterior spinal neural tube closure. Tmem132a-null mouse generation, neural tube phenotype analysis, cell migration assay, integrin expression/activation analysis by Western blot and immunofluorescence, loss-of-function in cell models Development (Cambridge, England) High 35950911
2022 Knockdown of TMEM132A in gastric cancer cells suppressed cell proliferation, migration, and invasion, whereas overexpression had opposite effects. These phenotypes were reversed by the Wnt activator LiCl or the Wnt inhibitor ICG-001, placing TMEM132A upstream of Wnt/β-catenin signaling in gastric cancer cell malignancy. In vivo knockdown suppressed GC tumorigenesis. siRNA knockdown, overexpression, CCK-8 proliferation assay, migration/invasion assays, LiCl/ICG-001 pharmacological rescue, xenograft tumor model Nucleosides, nucleotides & nucleic acids Medium 36441075
2023 In Tmem132a mutant mice, visceral endoderm fails to be excluded from the medial hindgut region, leading to loss or malformation of cloaca-derived genitourinary and gastrointestinal structures, and indirectly to neural tube and kidney/ureter defects. Co-immunoprecipitation demonstrated that TMEM132A physically interacts with the planar cell polarity (PCP) regulators CELSR1 and FZD6. Genetic epistasis showed that Tmem132a regulates neural tube closure synergistically with the PCP regulator Vangl2, identifying TMEM132A as a new regulator of PCP. Tmem132a mutant mouse analysis (histology, immunofluorescence), co-immunoprecipitation of TMEM132A with CELSR1 and FZD6, genetic double-mutant epistasis with Vangl2 Development (Cambridge, England) High 37390294
2024 TMEM132A directly interacts with the Wnt co-receptor LRP6, preventing its lysosomal degradation and thereby stabilizing LRP6 protein levels. Loss of Tmem132a in mouse embryos led to diminished canonical Wnt/β-catenin signaling and developmental defects including open spina bifida, caudal truncation, syndactyly, and renal defects. Tmem132a knockdown in cultured cells suppressed canonical Wnt/β-catenin signaling, reversible by LRP6 stabilization. Co-immunoprecipitation of TMEM132A with LRP6, lysosomal inhibition rescue assay, Tmem132a knockout mouse embryo analysis, Wnt/β-catenin reporter assay, knockdown in cultured cells Cell communication and signaling : CCS High 39385148
2024 E2F1 transcription factor directly regulates TMEM132A expression by binding to the TMEM132A promoter, as demonstrated by dual-luciferase reporter assay. Interference with E2F1 or TMEM132A expression significantly inhibited prostate cancer cell proliferation, suggesting E2F1 drives cancer cell growth in part through transcriptional activation of TMEM132A. Dual luciferase reporter assay (E2F1 binding to TMEM132A promoter), qPCR, siRNA knockdown of E2F1 and TMEM132A, CCK-8 proliferation assay, TCGA database analysis Frontiers in bioscience (Landmark edition) Medium 39473405
2026 Autoantibodies (IgG) targeting TMEM132A were identified in serum samples from patients with autoimmune cerebellar ataxia (ACA). TMEM132A protein was shown to be enriched in the central nervous system by immunohistochemical and immunocytochemical assays. Immunoprecipitation and mass spectrometry confirmed TMEM132A as the target antigen, and a cell-based binding assay validated TMEM132A-IgG in patient serum, indicating TMEM132A is a CNS-enriched surface-accessible antigen relevant to autoimmune disease. Immunohistochemistry, immunocytochemistry, immunoprecipitation, mass spectrometry, cell-based binding assay (CBA) Journal of neuroimmunology Medium 41564469

Source papers

Stage 0 corpus · 107 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2012 Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell 1718 22658674
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2008 Global analysis of host-pathogen interactions that regulate early-stage HIV-1 replication. Cell 787 18854154
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
1998 GBP, an inhibitor of GSK-3, is implicated in Xenopus development and oncogenesis. Cell 284 9635432
1999 Interferon-induced guanylate binding protein-1 (GBP-1) mediates an antiviral effect against vesicular stomatitis virus and encephalomyocarditis virus. Virology 224 10087221
2016 Guanylate Binding Protein (GBP) 5 Is an Interferon-Inducible Inhibitor of HIV-1 Infectivity. Cell host & microbe 213 26996307
2013 Why do cellular proteins linked to K63-polyubiquitin chains not associate with proteasomes? The EMBO journal 213 23314748
2001 The helical domain of GBP-1 mediates the inhibition of endothelial cell proliferation by inflammatory cytokines. The EMBO journal 158 11598000
2013 IRG and GBP host resistance factors target aberrant, "non-self" vacuoles characterized by the missing of "self" IRGM proteins. PLoS pathogens 154 23785284
2000 Interaction among GSK-3, GBP, axin, and APC in Xenopus axis specification. The Journal of cell biology 139 10684251
2013 In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine. Proteomics 138 23533145
1995 Interferon regulatory factor 1 is required for mouse Gbp gene activation by gamma interferon. Molecular and cellular biology 138 7823961
2006 In silico genomic analysis of the human and murine guanylate-binding protein (GBP) gene clusters. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 131 16689661
2010 Whole exome capture in solution with 3 Gbp of data. Genome biology 130 20565776
2001 Physiological regulation of [beta]-catenin stability by Tcf3 and CK1epsilon. The Journal of cell biology 126 11524435
2011 Determinants of GBP recruitment to Toxoplasma gondii vacuoles and the parasitic factors that control it. PloS one 109 21931713
2012 GBP-1 acts as a tumor suppressor in colorectal cancer cells. Carcinogenesis 90 23042300
2000 Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA research : an international journal for rapid publication of reports on genes and genomes 88 10997877
2017 The STUbL RNF4 regulates protein group SUMOylation by targeting the SUMO conjugation machinery. Nature communications 86 29180619
2002 Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs. Genome research 82 12421765
2014 The E2-like conjugation enzyme Atg3 promotes binding of IRG and Gbp proteins to Chlamydia- and Toxoplasma-containing vacuoles and host resistance. PloS one 77 24466199
2018 Constitutive Interferon Maintains GBP Expression Required for Release of Bacterial Components Upstream of Pyroptosis and Anti-DNA Responses. Cell reports 70 29972777
2002 The regulation of glycogen synthase kinase-3 nuclear export by Frat/GBP. The Journal of biological chemistry 70 12223487
1998 Beta-galactoside-binding protein (beta GBP) alters the cell cycle, up-regulates expression of the alpha- and beta-chains of the IFN-gamma receptor, and triggers IFN-gamma-mediated apoptosis of activated human T lymphocytes. Journal of immunology (Baltimore, Md. : 1950) 69 9725202
2008 Analyses of murine GBP homology clusters based on in silico, in vitro and in vivo studies. BMC genomics 66 18402675
2015 Temporal proteomics of NGF-TrkA signaling identifies an inhibitory role for the E3 ligase Cbl-b in neuroblastoma cell differentiation. Science signaling 61 25921289
2014 Switching between humoral and cellular immune responses in Drosophila is guided by the cytokine GBP. Nature communications 54 25130174
2002 Glycogen synthase kinase-3 beta mutagenesis identifies a common binding domain for GBP and Axin. The Journal of biological chemistry 54 11861647
2003 GBP binds kinesin light chain and translocates during cortical rotation in Xenopus eggs. Development (Cambridge, England) 52 14507779
2012 IFN-γ and TNF-α-induced GBP-1 inhibits epithelial cell proliferation through suppression of β-catenin/TCF signaling. Mucosal immunology 51 22692453
2023 The exoprotein Gbp of Fusobacterium nucleatum promotes THP-1 cell lipid deposition by binding to CypA and activating PI3K-AKT/MAPK/NF-κB pathways. Journal of advanced research 50 37100345
2018 TMEM132: an ancient architecture of cohesin and immunoglobulin domains define a new family of neural adhesion molecules. Bioinformatics (Oxford, England) 48 29088312
2002 Murine GBP-5, a new member of the murine guanylate-binding protein family, is coordinately regulated with other GBPs in vivo and in vitro. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 46 12396730
2004 GBP-5 splicing variants: New guanylate-binding proteins with tumor-associated expression and antigenicity. The Journal of investigative dermatology 42 15175044
1999 Cell cycle arrest and induction of apoptosis by beta galactoside binding protein (beta GBP) in human mammary cancer cells. A potential new approach to cancer control. European journal of cancer (Oxford, England : 1990) 41 10533482
2023 Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders. Cell genomics 38 36950384
2021 Interferon-stimulated GTPases in autoimmune and inflammatory diseases: promising role for the guanylate-binding protein (GBP) family. Rheumatology (Oxford, England) 36 33159795
1999 Negative cell cycle control of human T cells by beta-galactoside binding protein (beta GBP): induction of programmed cell death in leukaemic cells. Journal of cellular physiology 35 9886496
1999 Serodiagnosis of Leishmania donovani infections: assessment of enzyme-linked immunosorbent assays using recombinant L. donovani gene B protein (GBP) and a peptide sequence of L. donovani GBP. Transactions of the Royal Society of Tropical Medicine and Hygiene 34 10450438
1994 Identification and characterization of a novel GGA/C-binding protein, GBP-i, that is rapidly inducible by cytokines. Molecular and cellular biology 34 7969118
2020 TMEM132A, a Novel Wnt Signaling Pathway Regulator Through Wntless (WLS) Interaction. Frontiers in cell and developmental biology 33 33324648
1998 Murine GBP-2: a new IFN-gamma-induced member of the GBP family of GTPases isolated from macrophages. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 33 9858320
2003 Cloning and characterization of a novel GRP78-binding protein in the rat brain. The Journal of biological chemistry 31 12514190
2022 Assembled and annotated 26.5 Gbp coast redwood genome: a resource for estimating evolutionary adaptive potential and investigating hexaploid origin. G3 (Bethesda, Md.) 30 35100403
2022 A dual-activity topoisomerase complex regulates mRNA translation and turnover. Nucleic acids research 25 35748872
1991 A Plasmodium falciparum blood stage antigen highly homologous to the glycophorin binding protein GBP. Molecular and biochemical parasitology 25 1775169
2022 ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1. Proceedings of the National Academy of Sciences of the United States of America 22 35696571
1985 Interferon-induced guanylate-binding proteins: mapping of the murine Gbp-1 locus to chromosome 3. Virology 22 3927587
2000 Gabapentin-lactam (8-aza-spiro[5,4]decan-9-on; GBP-L) inhibits oxygen glucose deprivation-induced [3H]glutmate release and is a neuroprotective agent in amodel of acute retinal ischemia. Naunyn-Schmiedeberg's archives of pharmacology 21 10935536
2019 Molecular identification and antiviral function of the guanylate-binding protein (GBP) genes in the Chinese tree shrew (Tupaia belangeri chinesis). Developmental and comparative immunology 20 30817937
2021 A novel p53 regulator, C16ORF72/TAPR1, buffers against telomerase inhibition. Aging cell 19 33660365
2019 Effects of GLP-1 on counter-regulatory responses during hypoglycemia after GBP surgery. European journal of endocrinology 19 31176298
2019 Guanylate binding protein 1 (GBP-1) promotes cell motility and invasiveness of lung adenocarcinoma. Biochemical and biophysical research communications 19 31421831
2021 Transcriptional enhancement of GBP-5 by BATF aggravates sepsis-associated liver injury via NLRP3 inflammasome activation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 18 34042221
2018 IFN-γ-response mediator GBP-1 represses human cell proliferation by inhibiting the Hippo signaling transcription factor TEAD. The Biochemical journal 18 30120107
1999 Interleukin-10 gene transfer activates interferon-gamma and the interferon-gamma-inducible genes Gbp-1/Mag-1 and Mig-1 in mammary tumors. International journal of cancer 18 9935167
2024 iNOS is necessary for GBP-mediated T. gondii clearance in murine macrophages via vacuole nitration and intravacuolar network collapse. Nature communications 17 38538595
1996 Rat p67 GBP is induced by interferon-gamma and isoprenoid-modified in macrophages. Biochemical and biophysical research communications 17 8702422
2022 Evolution of Guanylate Binding Protein (GBP) Genes in Muroid Rodents (Muridae and Cricetidae) Reveals an Outstanding Pattern of Gain and Loss. Frontiers in immunology 16 35222365
2021 Evolution of the guanylate binding protein (GBP) genes: Emergence of GBP7 genes in primates and further acquisition of a unique GBP3 gene in simians. Molecular immunology 16 33550067
2000 A GBP 130 derived peptide from Plasmodium falciparum binds to human erythrocytes and inhibits merozoite invasion in vitro. Memorias do Instituto Oswaldo Cruz 14 10904405
2022 TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration. Development (Cambridge, England) 13 35950911
2017 Role of purified β-1, 3 glucan binding protein (β-GBP) from Paratelphusa hydrodromus and their anti-inflammatory, antioxidant and antibiofilm properties. Fish & shellfish immunology 13 28684323
2006 GRP78-binding protein regulates cAMP-induced glial fibrillary acidic protein expression in rat C6 glioblastoma cells. FEBS letters 12 16806201
1997 Plasma (1-->3)-beta-D-glucan assay and immunohistochemical staining of (1-->3)-beta-D-glucan in the fungal cell walls using a novel horseshoe crab protein (T-GBP) that specifically binds to (1-->3)-beta-D-glucan. Journal of clinical laboratory analysis 12 9058244
2024 Extracellular proximal interaction profiling by cell surface-targeted TurboID reveals LDLR as a partner of liganded EGFR. Science signaling 11 39499777
2017 A study on β-glucan binding protein (β-GBP) and its involvement in phenoloxidase cascade in Indian white shrimp Fenneropenaeus indicus. Molecular immunology 11 29028485
2017 20-Hydroxyecdysone promotes release of GBP-binding protein from oenocytoids to suppress hemocytic encapsulation. Insect biochemistry and molecular biology 11 29175381
1989 Plasmodium falciparum: an intervening sequence in the GBP 130/96 tR gene. Experimental parasitology 11 2659375
2025 The solute carrier superfamily interactome. Molecular systems biology 9 40355756
2023 Comparative study of GBP recruitment on two cytosol-dwelling pathogens, Francisella novicida and Shigella flexneri highlights differences in GBP repertoire and in GBP1 motif requirements. Pathogens and disease 8 37012222
2012 Activation of PLC by an endogenous cytokine (GBP) in Drosophila S3 cells and its application as a model for studying inositol phosphate signalling through ITPK1. The Biochemical journal 8 22928859
2002 Structure and activity of insect cytokine GBP which stimulates the EGF receptor. Molecules and cells 8 12243337
2022 Knockdown of TMEM132A restrains the malignant phenotype of gastric cancer cells via inhibiting Wnt signaling. Nucleosides, nucleotides & nucleic acids 7 36441075
2021 The Large GTPase, GBP-2, Regulates Rho Family GTPases to Inhibit Migration and Invadosome Formation in Breast Cancer Cells. Cancers 7 34830789
2016 Heparin-Promoted Cellular Uptake of the Cell-Penetrating Glycosaminoglycan Binding Peptide, GBPECP, Depends on a Single Tryptophan. ACS chemical biology 7 27936565
2024 Multidimensional analysis of TMEM132A in pan-cancer: unveiling its potential as a biomarker for treatment response prediction. Journal of Cancer 6 38947398
2014 Interaction investigations of crustacean β-GBP recognition toward pathogenic microbial cell membrane and stimulate upon prophenoloxidase activation. Journal of molecular recognition : JMR 6 24591174
2024 TMEM132A regulates Wnt/β-catenin signaling through stabilizing LRP6 during mouse embryonic development. Cell communication and signaling : CCS 5 39385148
2023 TMEM132A regulates mouse hindgut morphogenesis and caudal development. Development (Cambridge, England) 5 37390294
2024 Study of the Role of E2F1 and TMEM132A in Prostate Cancer Development. Frontiers in bioscience (Landmark edition) 4 39473405
2025 The surface protein Gbp of Fusobacterium nucleatum inhibits osteogenic differentiation by inactivating the Wnt/β-catenin pathway via binding to Annexin A2. Journal of translational medicine 3 40369630
2023 Advances in the regulation of inflammasome activation by GBP family in infectious diseases. Yi chuan = Hereditas 3 38764266
2021 Stress-derived reactive oxygen species enable hemocytes to release activator of growth blocking peptide (GBP) processing enzyme. Journal of insect physiology 3 33736983
2018 The Drosophila cytokine, GBP: A model that illuminates the yin-yang of inflammation and longevity in humans? Cytokine 3 29456061
2011 Quantitative analysis of energy transfer between fluorescent proteins in CFP-GBP-YFP and its response to Ca2+. Physical chemistry chemical physics : PCCP 3 21892487
2025 ESM-1 and GBP-1 are associated with endothelial dysfunction: emerging biomarkers or key players in the pathophysiology of preeclampsia? The Journal of physiology 2 40042932
2025 Toxoplasma gondii virulence in mice is determined by the pseudokinase ROP5B and countered by an IRG-GBP protein interplay. Frontiers in immunology 2 40703524
2021 Differential expression of serum GBP-28, NBP-Cyc 3 and TIMP-1 complicates pregnancy in hypertensive disorder pregnancy. Journal of reproductive immunology 2 33601303
2021 GFP-Tagged Protein Detection by Electron Microscopy Using a GBP-APEX Tool in Drosophila. Frontiers in cell and developmental biology 2 34476234
2018 A genome-wide dsRNA library screen for Drosophila genes that regulate the GBP/phospholipase C signaling axis that links inflammation to aging. BMC research notes 2 30545410
2025 Are environmental levels of gabapentin (GBP) a cause for concern? Chronic reproductive effects of GBP in zebrafish. Journal of hazardous materials 1 40188539
2025 Inter-kingdom signaling by the Legionella autoinducer LAI-1 involves the antimicrobial guanylate binding protein GBP. PLoS pathogens 1 40300029
2024 The Large GTPase Guanylate-Binding Protein-1 (GBP-1) Promotes Mitochondrial Fission in Glioblastoma. International journal of molecular sciences 1 39457021
2006 N-terminal mutational analysis of the interaction between growth-blocking peptide (GBP) and receptor of insect immune cells. Protein and peptide letters 1 17073728
2026 TMEM132A autoimmunity in patients with suspected autoimmune cerebellar ataxia. Journal of neuroimmunology 0 41564469
2026 TMEM132A is associated with metabolic reprogramming, macrophage-oriented immune remodeling, and breast cancer progression. Clinical and experimental medicine 0 41874757
2025 Human guanylate-binding protein (GBP) 1 inhibits replication of severe acute respiratory syndrome coronavirus 2. Journal of virology 0 40952259
2025 Fusobacterium nucleatum drives gastric cancer metastasis via Gbp-CypA-NF-κB-mediated CXCL8 crosstalk between tumor cells and mast cells. Cell communication and signaling : CCS 0 41316398
2023 Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated T. gondii restriction in murine macrophages via vacuole nitration and intravacuolar network collapse. bioRxiv : the preprint server for biology 0 37546987
2021 Using GBP Nanotrap to Restore Autophagy in the Rab5/Vps21 Mutant by Forcing Snf7 and Atg17 Interaction. Methods in molecular biology (Clifton, N.J.) 0 34453717