Affinage

Showing TCIM — TC1 is a alias.

TCIM

Transcriptional and immune response regulator · UniProt Q9NR00

Length
106 aa
Mass
12.3 kDa
Annotated
2026-06-10
84 papers in source corpus 17 papers cited in narrative 17 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

TCIM (TC-1/C8orf4) is a vertebrate-specific, intrinsically disordered 106-residue signaling adaptor that acts as a stress- and mitogen-inducible amplifier of proliferative, inflammatory, and pro-survival programs (PMID:11056052, PMID:15087392, PMID:19684084). Its central mechanistic role is positive regulation of Wnt/β-catenin signaling: it directly binds Chibby, a nuclear antagonist of β-catenin-mediated transcription, relieving Chibby's inhibition and enhancing expression of β-catenin target genes, with co-expression redistributing TCIM from the nucleolus to nuclear speckles where it colocalizes with Chibby (PMID:16424001). NMR analysis establishes that although TCIM is natively disordered, it carries three C-terminal regions of high helical propensity that mediate the Chibby interaction through transient helical structure (PMID:17905836). Beyond the Wnt axis, TCIM is induced by pro-inflammatory cytokines and cellular stress and acts within positive-feedback loops: it amplifies NF-κB signaling in endothelial cells by promoting RelA nuclear translocation and DNA binding and inducing inflammatory mediators (PMID:19684084), participates in a mutual-upregulation loop with HSF1 in the heat shock response (PMID:17603013), and binds directly to the COX-2 proximal promoter to drive COX-2/PGE2 expression in fibroblasts (PMID:26744410). TCIM is positioned downstream of FGFR2 and ERK1/2 mitogenic signaling, where it promotes the G1-to-S transition through cyclin D1 induction (PMID:17520678, PMID:18959821), and functions as a pro-survival factor that suppresses multiple death stimuli (PMID:28357300). In vivo, Tc1-knockout mice develop adipose hyperplasia with enhanced adipogenic stem-cell activity linked to canonical Wnt signaling (PMID:27775060), and C8orf4 expression is itself tuned post-transcriptionally by a miR-30a/H19 ceRNA axis during adipogenesis (PMID:31026067).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2000 Medium

    Establishing that TCIM is a discrete, ubiquitously expressed gene overexpressed in thyroid carcinoma framed it as a candidate proliferation-associated factor rather than an artifact.

    Evidence Suppression subtractive hybridization, RACE, Northern, and FISH cloning of C8orf4 from papillary thyroid carcinoma

    PMID:11056052

    Open questions at the time
    • No protein-level function assigned at cloning
    • Regulatory motifs identified but not functionally tested
    • Disease association correlative, not causal
  2. 2004 High

    Biophysical and cellular characterization answered what kind of protein TCIM is, showing it is monomeric and natively disordered, is a PKA/PKC substrate, and confers transformation-associated phenotypes.

    Evidence Recombinant protein structural assessment, in vitro kinase assays, and stable transfection of normal thyroid cells

    PMID:15087392

    Open questions at the time
    • Functional consequence of PKA/PKC phosphorylation not mapped to sites
    • Mechanism linking disorder to kinase activation unresolved
  3. 2004 Low

    Identification of C8orf4 as a TGF-β-responsive gene downregulated in metastatic colon cancer hinted at context-dependent regulation but provided no mechanism.

    Evidence Expression microarray, Northern, and real-time RT-PCR in colon adenoma cells

    PMID:15185345

    Open questions at the time
    • No promoter or pathway experiments performed
    • Correlative expression only
    • Direction of regulation differs from oncogenic contexts, unexplained
  4. 2006 High

    Discovery of the direct Chibby interaction defined TCIM's core molecular mechanism as a relief of Wnt/β-catenin antagonism, transforming it from a correlated marker into a pathway regulator.

    Evidence Reciprocal Co-IP, immunofluorescence colocalization, and β-catenin reporter/target-gene assays in mammalian cells; correlative invasion/proliferation data in gastric cancer

    PMID:16424001 PMID:16740781

    Open questions at the time
    • Stoichiometry and affinity of TCIM–Chibby binding not quantified here
    • Whether displacement of Chibby is direct competition or allosteric unclear
  5. 2007 High

    NMR resolved how a disordered protein achieves specific binding, localizing the Chibby interaction to three transient C-terminal helical regions.

    Evidence 15N NMR spectroscopy, chemical shift and relaxation analysis, and titration with Chibby

    PMID:17905836

    Open questions at the time
    • No bound-state complex structure
    • Helical residues not mutated to confirm necessity for function
  6. 2007 Medium

    Stress and FGFR2 epistasis experiments placed TCIM in inducible upstream signaling, showing it translocates to the nucleus upon stress, feeds back on HSF1, and lies downstream of FGFR2.

    Evidence qRT-PCR, immunofluorescence nuclear-translocation, siRNA knockdown across cell lines; pharmacological FGFR inhibition and forced FGFR2 expression with soft-agar assays

    PMID:17520678 PMID:17603013

    Open questions at the time
    • Nuclear import mechanism unknown
    • HSF1 feedback shown by mutual upregulation but not direct interaction
    • Intermediate steps between FGFR2 and TCIM induction undefined
  7. 2008 Medium

    Linking TCIM to ERK1/2-driven cyclin D1 induction explained how its mitogenic upregulation translates into cell-cycle progression.

    Evidence qRT-PCR, ERK1/2 inhibition, flow-cytometry cell-cycle analysis, and cyclin D1 promoter luciferase reporter

    PMID:18959821

    Open questions at the time
    • Whether TCIM acts directly on the cyclin D1 promoter or via β-catenin not distinguished
    • ERK1/2-to-TCIM link epistatic, not biochemical
  8. 2009 High

    Reciprocal gain/loss-of-function in endothelial cells established TCIM as an NF-κB amplifier acting through a positive-feedback loop, broadening its role into inflammation.

    Evidence Endothelial transfection/knockdown, ELISA, qRT-PCR, NF-κB translocation imaging, EMSA, adhesion/permeability assays, and zebrafish overexpression

    PMID:19684084

    Open questions at the time
    • Molecular target of TCIM within the NF-κB cascade not identified
    • Direct binding partner mediating RelA effects unknown
  9. 2013 Medium

    Reciprocal modulation in lung cancer cells reinforced the TCIM–Wnt/β-catenin link in a new tumor context.

    Evidence Lentiviral overexpression/knockdown with MTT, invasion, migration assays, qRT-PCR and Western blot of Wnt targets in A549 cells

    PMID:23880650

    Open questions at the time
    • Pathway readouts correlative, no direct mechanistic test
    • Does not separate Wnt from NF-κB contributions
  10. 2015 Medium

    Heterologous yeast expression provided a clean test of intrinsic pro-survival activity and confirmed TCIM's vertebrate-specific origin.

    Evidence Expression of human TC-1 in S. cerevisiae with copper and pro-apoptotic gene challenge plus bioinformatic ortholog analysis

    PMID:28357300

    Open questions at the time
    • Yeast lacks Chibby/Wnt machinery, so mechanism of survival in this system unidentified
    • Relevance to mammalian apoptosis pathways inferred
  11. 2016 High

    ChIP demonstrated TCIM is a direct transcriptional regulator of COX-2, and a knockout mouse plus radiation epistasis defined its in vivo physiological roles and confirmed Wnt dependence.

    Evidence ChIP, siRNA, PGE2 ELISA and methylation analysis in fibroblasts; Tc1-/- mouse phenotyping with β-catenin reporter; siRNA+XAV939 epistasis and xenografts

    PMID:26744410 PMID:27029901 PMID:27775060

    Open questions at the time
    • How a disordered protein binds the COX-2 promoter (direct DNA contact vs adaptor) unresolved
    • Tissue specificity of Wnt-dependent phenotypes not fully mapped
  12. 2019 Medium

    Defining a miR-30a/H19 ceRNA axis explained how C8orf4 levels are set post-transcriptionally during adipogenic differentiation.

    Evidence TargetScan, 3'-UTR luciferase reporter, miRNA/lncRNA over/knockdown rescue, and adipogenic differentiation assays in hADSCs

    PMID:31026067

    Open questions at the time
    • Other regulators of C8orf4 mRNA stability not surveyed
    • Connection between this axis and downstream Wnt/PPARγ program inferred

Open questions

Synthesis pass · forward-looking unresolved questions
  • The biochemical basis by which a single disordered protein integrates Wnt, NF-κB, HSF1, and COX-2 outputs—and whether phosphorylation switches between these activities—remains unresolved.
  • No structure of TCIM bound to any partner other than NMR-mapped Chibby helices
  • Functional role of PKA/PKC phosphorylation sites uncharacterized
  • Whether COX-2 promoter occupancy reflects direct DNA binding or recruitment is unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060089 molecular transducer activity 4 GO:0098772 molecular function regulator activity 3 GO:0140110 transcription regulator activity 2
Localization
GO:0005634 nucleus 3 GO:0005654 nucleoplasm 2 GO:0005730 nucleolus 1
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-168256 Immune System 2 R-HSA-74160 Gene expression (Transcription) 2 R-HSA-8953897 Cellular responses to stimuli 2 R-HSA-1640170 Cell Cycle 1
Partners
CBY1HSF1

Evidence

Reading pass · 17 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 TC-1 (C8orf4) was cloned as a novel gene overexpressed in papillary thyroid carcinoma; the full-length mRNA encodes a 106-amino-acid protein that is ubiquitously expressed in human tissues, localizes to chromosome 8p11.2, and three regulatory motifs were identified in its 5' flanking sequence. Suppression subtractive hybridization, RT-PCR, Northern analysis, 5'-RACE, primer extension, fluorescence in situ hybridization (FISH) Genomics Medium 11056052
2004 TC-1 (C8orf4) protein is monomeric and predominantly unstructured (natively disordered) at physiological salt and pH. Recombinant TC-1 can be phosphorylated in vitro by cyclic AMP-dependent protein kinase (PKA) and protein kinase C (PKC), and stable transfection of TC-1 into normal thyroid cells upregulates the activity of both kinases while conferring increased proliferation, anchorage-independent growth, and reduced apoptosis. Recombinant protein expression, in vitro kinase assay, stable transfection of normal thyroid cells, soft-agar colony formation, apoptosis assay Cancer research High 15087392
2006 TC1 (C8orf4) functions as a positive regulator of the Wnt/β-catenin pathway by directly interacting with Chibby (Cby), a nuclear antagonist of β-catenin-mediated transcription. TC1 binding to Cby relieves its inhibitory effect, enhancing β-catenin target gene expression (including MMP-7, MMP-14, laminin γ2). Upon co-expression, TC1 redistributes from nucleolus to nuclear speckles where it co-localizes with Cby. Co-immunoprecipitation, co-localization by immunofluorescence, β-catenin reporter assay, RT-PCR for target genes, mammalian cell transfection Cancer research High 16424001
2006 TC1 (C8orf4) expression correlates with Wnt/β-catenin target genes (laminin γ2, MMP-7, MMP-14, cyclin D1, c-Met, CD44) in gastric cancer tissue; overexpression in MKN45 cells enhances Matrigel invasiveness and proliferation, and TC1 expression increases after serial peritoneal seeding in nude mice. Tissue microarray with IHC, Matrigel invasion assay, proliferation assay, serial in vivo peritoneal seeding Clinical cancer research Medium 16740781
2006 TC1 (C8orf4) is upregulated by pro-inflammatory cytokines IL-1β and TNF-α in follicular dendritic cells (FDC-like HK line) and enhances their proliferation; TC1 knockdown inhibits IL-1β-induced proliferation, placing TC1 downstream of these cytokines in FDC proliferation regulation. RT-PCR, siRNA knockdown, cell proliferation assay in HK cells FEBS letters Medium 16730711
2007 NMR spectroscopy showed that TC-1 is intrinsically disordered but adopts compact conformations with three regions of high helical propensity (D44–R53, K58–A64, D73–T88) in its C-terminal portion. Upon addition of Chibby, significant resonance broadening from these helical regions indicates that TC-1 interacts with Cby through its transient helical structure. NMR spectroscopy (15N-labeled protein), chemical shift analysis, relaxation measurements, titration with Cby Protein science High 17905836
2007 TC1 (C8orf4) is upregulated by heat shock and other cellular stresses (H2O2, TPA, LPS, UV), and upon upregulation TC1 protein translocates into the nucleus independently of NF-κB activation. TC1 upregulates heat shock proteins, TC1 knockdown inhibits stress-induced downstream regulation, and TC1 and HSF1 mutually upregulate each other, suggesting a positive feedback loop in heat shock response. qRT-PCR, Western blot, immunofluorescence for nuclear translocation, siRNA knockdown, reporter/expression analysis in multiple cell lines Biochemical and biophysical research communications Medium 17603013
2007 TC-1 overexpression in human mammary epithelial (HME) cells confers anchorage-independent and growth-factor-independent proliferation. TC-1 expression is downregulated by the FGFR inhibitor PD173074 in FGFR2-amplified SUM-52 breast cancer cells, and forced FGFR2 expression in HME cells increases endogenous TC-1 mRNA, placing TC-1 downstream of FGFR2 signaling. Stable transfection, soft-agar colony formation, pharmacological inhibition (PD173074), forced FGFR2 expression, RT-PCR International journal of cancer Medium 17520678
2008 TC1 (C8orf4) mRNA is upregulated by mitogens (FBS/thrombin) at least partially via the ERK1/2 signaling pathway. Overexpression of TC1 promotes G1-to-S phase transition and significantly increases cyclin D1 promoter-driven luciferase activity; this effect is delayed when ERK1/2 signaling is deficient. qRT-PCR, ERK1/2 pathway inhibition, flow cytometry cell-cycle analysis, cyclin D1 promoter luciferase reporter assay BMB reports Medium 18959821
2009 TC1 (C8orf4) is upregulated by IL-1β, TNF-α, LPS, and phorbol ester in human aortic endothelial cells and umbilical vein endothelial cells, and this upregulation is blocked by IκB-kinase inhibitors. TC1 overexpression upregulates inflammatory mediators (IL-6, IL-1α, COX-2, CXCL1, CCL2, CCL5, IL-8, ICAM1, VCAM1, E-selectin) and enhances nuclear translocation of RelA and NF-κB DNA-binding activity, suggesting TC1 amplifies NF-κB signaling via positive feedback. TC1 knockdown downregulates these inflammatory parameters. Zebrafish TC1 overexpression induces edema. Endothelial cell transfection/knockdown, ELISA, qRT-PCR, NF-κB nuclear translocation assay (immunofluorescence), EMSA (DNA-binding), monocyte adhesion assay, permeability assay, zebrafish overexpression Journal of immunology High 19684084
2004 C8orf4 is induced by TGF-β signaling in colon adenoma cells (Vaco 330) and is downregulated in metastatic colon cancer, placing it as a TGF-β-responsive gene that may participate in colon cell differentiation or growth regulation. Expression microarray, Northern analysis, real-time RT-PCR International journal of cancer Low 15185345
2013 TC-1 (C8orf4) overexpression in A549 non-small cell lung cancer cells promotes proliferation, invasion, and migration, and increases expression of Wnt/β-catenin downstream genes (VEGF, MMP-7, cyclin D1, c-myc, survivin) at mRNA and protein levels; TC-1 knockdown has opposite effects. Lentiviral overexpression/knockdown, MTT assay, Matrigel invasion, scratch-wound assay, qRT-PCR, Western blot The Journal of surgical research Medium 23880650
2015 Human TC-1 (hTC-1/C8orf4) expressed heterologously in yeast functions as a pro-survival protein: it suppresses cell death and growth inhibition induced by copper sulfate and blocks the deleterious effects of over-expressed pro-apoptotic proteins (YCA1, YBH3, NUC1, AIF1). TC-1 orthologs are restricted to jawed vertebrates with no ortholog detected in yeast, confirming its vertebrate-specific origin. Heterologous expression of hTC-1 in Saccharomyces cerevisiae, growth/viability assays with copper and pro-apoptotic gene overexpression, bioinformatic ortholog analysis Microbial cell Medium 28357300
2016 C8orf4 is hypermethylated and downregulated in fibrotic lung fibroblasts compared to controls. siRNA knockdown of C8orf4 in control fibroblasts downregulates COX-2 and PGE2 production, generating a fibrotic-like phenotype. Chromatin immunoprecipitation (ChIP) demonstrated that C8orf4 binds the proximal promoter of COX-2, establishing it as a direct transcriptional regulator of COX-2 expression. Bisulfite sequencing, methylation microarray, siRNA knockdown, ChIP assay, PGE2 ELISA, qRT-PCR, 5-aza-2'-deoxycytidine treatment Clinical science High 26744410
2016 Tc1-knockout (Tc1-/-) mice show adipose tissue hyperplasia with smaller, more numerous adipocytes, enhanced glucose tolerance, and reduced serum lipids. Adipocyte-derived stem cells (ADSCs) from Tc1-/- mice display enhanced proliferative and adipogenic capacity; PPARγ and CEBPα are robustly upregulated upon adipogenic induction, while Wisp2 and Dlk1 (adipogenesis inhibitors) are downregulated. TC1-transfected NIH3T3 cells show higher β-catenin reporter activity, linking canonical Wnt signaling to Tc1-dependent adipose regulation. Tc1 knockout mouse model, histomorphometry, glucose tolerance test, serum lipid measurement, ADSC isolation and differentiation, qRT-PCR, β-catenin reporter assay Scientific reports High 27775060
2016 TC-1 knockdown combined with radiation inhibits proliferation and induces apoptosis in NSCLC A549 cells more effectively than either treatment alone, and this is associated with inactivation of Wnt/β-catenin signaling; the Wnt/β-catenin inhibitor XAV939 phenocopies TC-1 knockdown, confirming epistasis. Combined TC-1 siRNA and radiation also caused significant tumor regression in A549 xenografts. siRNA knockdown, MTT assay, flow cytometry, Western blot for β-catenin pathway, XAV939 pharmacological inhibition, xenograft model Biology open Medium 27029901
2019 In human adipose-derived mesenchymal stem cells (hADSCs), miR-30a directly binds the 3'-UTR of C8orf4 to inhibit its expression (validated by luciferase reporter assay). The lncRNA H19 acts as a competing endogenous RNA (ceRNA) for miR-30a, thereby augmenting C8orf4 expression and promoting adipogenic differentiation. H19 knockdown suppresses C8orf4 expression and adipogenesis, which can be partially reversed by miR-30a inhibition. TargetScan prediction, luciferase reporter gene assay (3'-UTR binding), miRNA/lncRNA overexpression and knockdown, qRT-PCR, Western blot, adipogenic differentiation assay Journal of cellular physiology Medium 31026067

Source papers

Stage 0 corpus · 84 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2018 Circular RNA circZFR contributes to papillary thyroid cancer cell proliferation and invasion by sponging miR-1261 and facilitating C8orf4 expression. Biochemical and biophysical research communications 97 29842886
1985 Hematopoietic factor production by a cell line (TC-1) derived from adherent murine marrow cells. Blood 97 2410062
1996 Inhibition of TC-1 cytokine production, effector cytotoxic T lymphocyte development and alloantibody production by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Journal of immunology (Baltimore, Md. : 1950) 83 8805628
2003 Immunisation with modified HPV16 E7 genes against mouse oncogenic TC-1 cell sublines with downregulated expression of MHC class I molecules. Vaccine 77 12559790
2016 Epigenetic regulation of cyclooxygenase-2 by methylation of c8orf4 in pulmonary fibrosis. Clinical science (London, England : 1979) 67 26744410
2017 Engineered outer membrane vesicle is potent to elicit HPV16E7-specific cellular immunity in a mouse model of TC-1 graft tumor. International journal of nanomedicine 64 28979120
2004 TC-1 is a novel tumorigenic and natively disordered protein associated with thyroid cancer. Cancer research 60 15087392
2006 TC1 (C8orf4) enhances the Wnt/beta-catenin pathway by relieving antagonistic activity of Chibby. Cancer research 52 16424001
2012 Cyclophosphamide-induced myeloid-derived suppressor cell population is immunosuppressive but not identical to myeloid-derived suppressor cells induced by growing TC-1 tumors. Journal of immunotherapy (Hagerstown, Md. : 1997) 48 22576342
2000 Cloning of TC-1 (C8orf4), a novel gene found to be overexpressed in thyroid cancer. Genomics 48 11056052
2006 TC1(C8orf4) correlates with Wnt/beta-catenin target genes and aggressive biological behavior in gastric cancer. Clinical cancer research : an official journal of the American Association for Cancer Research 44 16740781
2014 Production of a novel multi-epitope peptide vaccine for cancer immunotherapy in TC-1 tumor-bearing mice. Biologicals : journal of the International Association of Biological Standardization 43 25467837
2009 Soluble B and T lymphocyte attenuator possesses antitumor effects and facilitates heat shock protein 70 vaccine-triggered antitumor immunity against a murine TC-1 cervical cancer model in vivo. Journal of immunology (Baltimore, Md. : 1950) 40 19923459
2020 Mannose-Modified Liposome Co-Delivery of Human Papillomavirus Type 16 E7 Peptide and CpG Oligodeoxynucleotide Adjuvant Enhances Antitumor Activity Against Established Large TC-1 Grafted Tumors in Mice. International journal of nanomedicine 32 33293808
2019 Vaccination with synthetic long peptide formulated with CpG in an oil-in-water emulsion induces robust E7-specific CD8 T cell responses and TC-1 tumor eradication. BMC cancer 31 31170937
2019 H19/miR-30a/C8orf4 axis modulates the adipogenic differentiation process in human adipose tissue-derived mesenchymal stem cells. Journal of cellular physiology 30 31026067
2015 Dendritic cells pulsed with tumor cells killed by high hydrostatic pressure induce strong immune responses and display therapeutic effects both in murine TC-1 and TRAMP-C2 tumors when combined with docetaxel chemotherapy. International journal of oncology 30 26718011
2009 TC1(C8orf4) is a novel endothelial inflammatory regulator enhancing NF-kappaB activity. Journal of immunology (Baltimore, Md. : 1950) 30 19684084
2018 Encapsulation of Poly I:C and the natural phosphodiester CpG ODN enhanced the efficacy of a hyaluronic acid-modified cationic lipid-PLGA hybrid nanoparticle vaccine in TC-1-grafted tumors. International journal of pharmaceutics 29 30393151
2011 A novel, broad spectrum therapeutic HPV vaccine targeting the E7 proteins of HPV16, 18, 31, 45 and 52 that elicits potent E7-specific CD8T cell immunity and regression of large, established, E7-expressing TC-1 tumors. Vaccine 28 21816200
2004 C8orf4 is a transforming growth factor B induced transcript downregulated in metastatic colon cancer. International journal of cancer 27 15185345
2018 Comparative Proteomic Study of the Antiproliferative Activity of Frog Host-Defence Peptide Caerin 1.9 and Its Additive Effect with Caerin 1.1 on TC-1 Cells Transformed with HPV16 E6 and E7. BioMed research international 26 29862288
1991 Transport and metabolism of glucose in an insulin-secreting cell line, beta TC-1. Biochemistry 26 1747375
2018 Synergy effects of Polyinosinic-polycytidylic acid, CpG oligodeoxynucleotide, and cationic peptides to adjuvant HPV E7 epitope vaccine through preventive and therapeutic immunization in a TC-1 grafted mouse model. Human vaccines & immunotherapeutics 25 29271696
2016 Chimeric HBcAg virus-like particles presenting a HPV 16 E7 epitope significantly suppressed tumor progression through preventive or therapeutic immunization in a TC-1-grafted mouse model. International journal of nanomedicine 25 27313455
2007 Transforming properties of TC-1 in human breast cancer: interaction with FGFR2 and beta-catenin signaling pathways. International journal of cancer 25 17520678
2007 The intrinsically disordered TC-1 interacts with Chibby via regions with high helical propensity. Protein science : a publication of the Protein Society 24 17905836
1990 Further studies on growth factor production by the TC-1 stromal cell line: pre-B stimulating activity. Blood 24 1693296
2014 IL-10 signalling blockade at the time of immunization inhibits Human papillomavirus 16 E7 transformed TC-1 tumour cells growth in mice. Cellular immunology 23 24983823
2006 TC1(C8orf4) is upregulated by IL-1beta/TNF-alpha and enhances proliferation of human follicular dendritic cells. FEBS letters 23 16730711
2014 Sequential treatment of HPV E6 and E7-expressing TC-1 cells with bortezomib and celecoxib promotes apoptosis through p-p38 MAPK-mediated downregulation of cyclin D1 and CDK2. Oncology reports 21 24627094
2013 TC-1 (c8orf4) enhances aggressive biologic behavior in lung cancer through the Wnt/β-catenin pathway. The Journal of surgical research 21 23880650
2016 Adipose tissue hyperplasia with enhanced adipocyte-derived stem cell activity in Tc1(C8orf4)-deleted mice. Scientific reports 20 27775060
2022 Characterization of a novel chitinolytic Serratia marcescens strain TC-1 with broad insecticidal spectrum. AMB Express 19 35907065
2021 Intratumoral injection of caerin 1.1 and 1.9 peptides increases the efficacy of vaccinated TC-1 tumor-bearing mice with PD-1 blockade by modulating macrophage heterogeneity and the activation of CD8+ T cells in the tumor microenvironment. Clinical & translational immunology 18 34429969
2011 Exploring platelet chemokine antimicrobial activity: nuclear magnetic resonance backbone dynamics of NAP-2 and TC-1. Antimicrobial agents and chemotherapy 18 21321145
2008 TC1 (C8orf4) is involved in ERK1/2 pathway-regulated G(1)- to S-phase transition. BMB reports 18 18959821
2016 Knockdown of TC-1 enhances radiosensitivity of non-small cell lung cancer via the Wnt/β-catenin pathway. Biology open 17 27029901
2015 The high expression of TC1 (C8orf4) was correlated with the expression of β-catenin and cyclin D1 and the progression of squamous cell carcinomas of the tongue. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 15 25869879
2013 Prevention and Inhibition of TC-1 Cell Growth in Tumor Bearing Mice by HPV16 E7 Protein in Fusion with Shiga Toxin B-Subunit from shigella dysenteriae. Cell journal 15 23862120
2006 Cell structure degradation in Escherichia coli and Thermococcus sp. strain Tc-1-95 associated with thermal death resulting from brief heat treatment. FEMS microbiology letters 15 16790024
1995 Interaction of glucagon-like peptide-I (GLP-I) and galanin in insulin (beta TC-1)- and somatostatin (RIN T3)-secreting cells and evidence that both peptides have no receptors on glucagon (INR1G9)-secreting cells. Acta diabetologica 15 8590787
2021 Topical Application of Temperature-Sensitive Gel Containing Caerin 1.1 and 1.9 Peptides on TC-1 Tumour-Bearing Mice Induced High-Level Immune Response in the Tumour Microenvironment. Frontiers in oncology 14 34858827
2012 Selenium enhances the efficacy of Radachlorin mediated-photodynamic therapy in TC-1 tumor development. Oncology reports 14 22614712
2007 TC1 (C8orf4) is upregulated by cellular stress and mediates heat shock response. Biochemical and biophysical research communications 13 17603013
2004 Chemically synthesized protein as tumour-specific vaccine: immunogenicity and efficacy of synthetic HPV16 E7 in the TC-1 mouse tumour model. Vaccine 13 15530672
2021 Intratumoral Injection of a Human Papillomavirus Therapeutic Vaccine-Induced Strong Anti-TC-1-Grafted Tumor Activity in Mice. Cancer management and research 12 34584459
2006 Molecular cloning of a Trypanosoma cruzi cell surface casein kinase II substrate, Tc-1, involved in cellular infection. Infection and immunity 12 16790765
2014 TC-1 overexpression promotes cell proliferation in human non-small cell lung cancer that can be inhibited by PD173074. PloS one 11 24941347
2013 TC-1 (C8orf4) expression is correlated with differentiation in ovarian carcinomas and might distinguish metastatic ovarian from metastatic colorectal carcinomas. Virchows Archiv : an international journal of pathology 11 23377761
2005 Characterization of cell lines derived from tumors induced by TC-1 cells in mice preimmunized against HPV16 E7 oncoprotein. International journal of oncology 11 16077923
2018 Evaluation of murine lung epithelial cells (TC-1 JHU-1) line to develop Th2-promoting cytokines IL-25/IL-33/TSLP and genes Tlr2/Tlr4 in response to Aspergillus fumigatus. Journal de mycologie medicale 9 29525270
2011 Vaccination with OK-432 followed by TC-1 tumor lysate leads to significant antitumor effects. Reproductive sciences (Thousand Oaks, Calif.) 9 21321245
2011 Immunization of protein HPV16 E7 in fusion with mouse HSP70 inhibits the growth of TC-1 cells in tumor bearing mice. Vaccine 9 21722685
2015 Immunization with mutant HPV16 E7 protein inhibits the growth of TC-1 cells in tumor-bearing mice. Oncology letters 8 25789055
2014 Co-Culture of α TC-6 Cells and β TC-1 Cells: Morphology and Function. Endocrinology and metabolism (Seoul, Korea) 8 25325280
2010 Investigation of copy-number variations of C8orf4 in hematological malignancies. Medical oncology (Northwood, London, England) 8 20878554
1988 Characterization of two clones isolated from the TC-1 murine marrow stromal cell line: growth factor and retrovirus production and physical support of hemopoiesis. International journal of cell cloning 8 3373034
2012 Synthesis of chlorin-based unsaturated fatty acid conjugates: their in vitro phototoxicity on TC-1 cancer cell line. Journal of photochemistry and photobiology. B, Biology 7 22487363
2024 Study on the biodegradation characteristics and mechanism of tetracycline by Serratia entomophila TC-1. The Science of the total environment 6 38960187
2018 Combined immunization against TGF-β1 enhances HPV16 E7-specific vaccine-elicited antitumour immunity in mice with grafted TC-1 tumours. Artificial cells, nanomedicine, and biotechnology 6 29929402
2007 [Effect of Nocardia rubra cell wall skeleton (Nr-CWS) on oncogenicity of TC-1 cells and anti-human papillomavirus effect of Nr-CWS in lower genital tract of women]. Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology 6 18322596
2022 Photo-sonodynamic therapy mediated with OLI_NPs to induce HPV16E7-specific immune response and inhibit cervical cancer in a Tc-1-grafted murine model. Journal of photochemistry and photobiology. B, Biology 5 36436360
2021 Mesenchymal stem cells support delivery and boost the efficacy of oncolytic reoviruses in TC-1 tumor cells. Journal of cellular biochemistry 5 34056765
2019 Association of C8orf4 expression with its methylation status, aberrant β-catenin expression, and the development of cervical squamous cell carcinoma. Medicine 5 31374065
2018 Constructing TC-1-GLUC-LMP2 Model Tumor Cells to Evaluate the Anti-Tumor Effects of LMP2-Related Vaccines. Viruses 5 29570629
2015 Lipocalin-type prostaglandin D2 synthase reduces glucagon secretion in alpha TC-1 clone 6 cells via the DP1 receptor. Biochemistry and biophysics reports 5 29124207
1996 Interaction between insulin-like growth factor-I and insulin-like growth factor-binding proteins in TC-1 stromal cells. The Journal of endocrinology 5 8691111
2024 The ability of clostridium novyi-NT spores to induce apoptosis via the mitochondrial pathway in mice with HPV-positive cervical cancer tumors derived from the TC-1 cell line. BMC complementary medicine and therapies 4 39732669
2017 Invasion of TC-1 cells to skeletal muscle fibers protect them from peroxisomicine A1 (T-514) treatment in a murine model of cancer. International journal of clinical and experimental pathology 4 31966659
2015 Human Thyroid Cancer-1 (TC-1) is a vertebrate specific oncogenic protein that protects against copper and pro-apoptotic genes in yeast. Microbial cell (Graz, Austria) 4 28357300
2003 Immunotherapy of HPV 16-associated tumours with tumour cell line/dendritic cell line (TC-1/DC2.4) hybrid vaccines. Folia biologica 4 14680295
2023 Anti-tumor Effects of Cisplatin Synergist in Combined Treatment with Clostridium novyi-NT Spores Against Hypoxic Microenvironments in a Mouse Model of Cervical Cancer Caused by TC-1 Cell Line. Advanced pharmaceutical bulletin 3 38022809
2009 Inhibition of TC-1 tumor progression by cotransfection of Saxatilin and IL-12 genes mediated by lipofection or electroporation. Oncology research 3 20225758
2024 Synergy Effects of HPV E6-E7 Encoding mRNA and Nucleic Acid Immunostimulators Improve Therapeutic Potential in TC-1 Graft Tumor. Journal of medical virology 2 39588712
2017 Fungal mannosylation enhances human papillomavirus 16 E7 therapeutic immunity against TC-1 tumors. Oncology reports 2 29115562
2018 The role of immune cell subpopulations in the growth and rejection of TC-1/A9 tumors in novel mouse strains differing in the H2-D haplotype and NKC domain. Oncology letters 1 29467880
1996 Control of renal carcinoma TC-1 growth, cyclin/kinase and nm23 expression by IFN-gamma and TGF-beta. Anticancer research 1 8712696
1993 [Demonstration of interleukin-1 (IL-1) as an autocrine growth inhibitor in renal cancer cell line, TC-1]. Nihon Hinyokika Gakkai zasshi. The japanese journal of urology 1 8411809
1992 [Establishment of a new human renal cancer cell line (TC-1) and its productivity of interleukin-6 (IL-6)]. Nihon Hinyokika Gakkai zasshi. The japanese journal of urology 1 1479760
2026 Evaluation of the Effects of Newcastle Disease Virus as an Oncolytic Virus on the Expression of Apoptosis-related Genes in TC-1 Cell Line. Iranian journal of allergy, asthma, and immunology 0 41674174
2019 The Dosage of the Derivative of Clostridium Ghonii (DCG) Spores Dictates Whether an IFNγ/IL-9 or a Strong IFNγ Response Is Elicited in TC-1 Tumour Bearing Mice. BioMed research international 0 31183361
2016 [Changes in the Expression of AKT and ERK after the JSRV-Env-Induced Transfection of TC-1 and TC-1-Hyal2 Cells]. Bing du xue bao = Chinese journal of virology 0 29962199
1982 [Morphological, cytogenetic and proliferative characteristics of Syrian hamster HTC-2 and HTC-1 cells and of the HTCT tumor cell line transformed by herpes simplex type 2 virus]. Voprosy virusologii 0 6291246

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