Affinage

SLC27A1

Long-chain fatty acid transport protein 1 · UniProt Q6PCB7

Length
646 aa
Mass
71.1 kDa
Annotated
2026-06-10
100 papers in source corpus 24 papers cited in narrative 24 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SLC27A1/FATP1 is an integral membrane very long-chain acyl-CoA synthetase that drives cellular uptake of long-chain fatty acids through esterification-coupled metabolic trapping (PMID:10593920, PMID:23024797). Its enzymatic activity preferentially activates very long-chain substrates such as C24:0 and requires two conserved catalytic domains, and the same protein supports both fatty acid transport and acyl-CoA synthetase function as established by complementation of the yeast fat1Δ strain (PMID:10593920, PMID:10880966). In insulin-responsive adipocytes and skeletal muscle, FATP1 translocates from an intracellular compartment to the plasma membrane upon insulin stimulation, and its loss abolishes insulin-stimulated fatty acid uptake, redistributes dietary lipid to the liver, and protects against diet-induced obesity and insulin resistance (PMID:16611988). Imported fatty acids are channeled toward triacylglycerol synthesis or, via FATP1 at the outer mitochondrial membrane/intermembrane space where it cooperates with CPT1, toward β-oxidation (PMID:12235169, PMID:19429947, PMID:24858472). The acyl-CoA synthetase reaction generates AMP that raises the AMP/ATP ratio and activates AMPK and acetyl-CoA carboxylase in response to insulin and adiponectin (PMID:19560442, PMID:20667975). FATP1 also has tissue-specific roles: it transports DHA bidirectionally across the blood-brain barrier under insulin and iron regulation (PMID:28035674, PMID:39044044), interacts via its cytosolic C-terminus with the visual-cycle proteins RPE65 and LRAT to inhibit 11-cis retinol production (PMID:20356843), shapes macrophage substrate use and inflammatory phenotype (PMID:27408776), and is exploited by melanoma and other cancers to acquire fatty acids and promote growth and invasion (PMID:29903879, PMID:31575907).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 1999 High

    Established that FATP1 is itself an enzyme — a very long-chain acyl-CoA synthetase — rather than a passive carrier, defining esterification-coupled influx as the proposed uptake mechanism.

    Evidence Acyl-CoA synthetase assays with C24:0/C16:0 and active-site mutagenesis of FATP1-Myc/His expressed in COS1 cells

    PMID:10593920

    Open questions at the time
    • Did not resolve whether transport occurs by direct membrane translocation or solely metabolic trapping
    • No structural model of the catalytic domains
  2. 2000 High

    Demonstrated that a single FATP1 protein carries both fatty acid transport and acyl-CoA synthetase/β-oxidation functions, conserved with yeast Fat1p, with chain-length selectivity.

    Evidence Genetic complementation of S. cerevisiae fat1Δ with murine FATP1 plus transport, synthetase, and β-oxidation assays

    PMID:10880966

    Open questions at the time
    • Did not separate transport from synthetase activity mechanistically
    • Mammalian tissue context untested
  3. 2000 Medium

    Defined the human SLC27A1 gene structure, chromosomal locus, and tissue expression bias toward muscle and adipose, framing where FATP1 acts.

    Evidence cDNA/genomic cloning, FISH mapping to 19p13.1, and Northern blot expression

    PMID:10873384

    Open questions at the time
    • Expression survey is descriptive, not functional
    • No protein-level tissue quantification
  4. 2005 High

    Showed that FATP isoforms divide transport versus synthetase roles, and that FATP1 can stimulate fatty acid uptake and divert it to TG synthesis even from intracellular membranes, challenging a strict plasma-membrane transporter model.

    Evidence Six-isoform yeast complementation; adenoviral EGFP-FATP1 in human myotubes with localization and transport/oxidation/esterification assays

    PMID:15699031 PMID:15897321

    Open questions at the time
    • Localization in primary myotubes may differ from native tissue
    • Mechanism reconciling intracellular localization with surface uptake unresolved
  5. 2006 High

    Established FATP1 as the insulin-sensitive fatty acid transporter in vivo: insulin drives its translocation to the plasma membrane, and its loss abolishes insulin-stimulated uptake and protects against metabolic disease.

    Evidence FATP1-null mice with insulin-stimulated uptake, translocation fractionation, and diet-induced obesity phenotyping

    PMID:16611988

    Open questions at the time
    • Molecular machinery of insulin-driven translocation not identified
    • Compensation by other FATP/ACSL isoforms in muscle not fully resolved
  6. 2009 High

    Placed FATP1 at the mitochondrion as a partner of CPT1, linking fatty acid import to β-oxidation, and showed FATP1 can also stimulate glucose oxidation via PDH.

    Evidence Co-IP of FATP1 with CPT1 from myotubes and rat muscle, etomoxir-sensitive oxidation assays, and subcellular fractionation/PDH activity measurements

    PMID:19361580 PMID:19429947

    Open questions at the time
    • Reciprocal Co-IP and stoichiometry of the FATP1–CPT1 interaction not defined
    • Mechanism by which FATP1 activates PDH-E1β unknown
  7. 2010 Medium

    Identified FATP1 acyl-CoA synthetase activity as the source of AMP that activates AMPK signaling downstream of insulin and adiponectin.

    Evidence shRNA/siRNA knockdown of FATP1 (with ACSL1) in adipocytes, AMP/ATP ratio measurement, and phospho-AMPK/ACC immunoblots

    PMID:19560442 PMID:20667975

    Open questions at the time
    • Relative contribution of FATP1 versus ACSL1 to the AMP pool not separated
    • Single lab
  8. 2010 Medium

    Revealed a non-metabolic moonlighting role: FATP1 binds RPE65 and LRAT via its cytosolic C-terminus to inhibit the retinal visual cycle.

    Evidence Yeast two-hybrid with RPE65, dose-dependent interaction with native RPE65/LRAT, and 11-cis retinol production assays in RPE

    PMID:20356843

    Open questions at the time
    • Physiological significance in vivo not established by this study
    • Whether inhibition depends on FATP1 enzymatic activity unknown
  9. 2011 High

    Confirmed in vivo that FATP1 can channel muscle fatty acids toward oxidation rather than storage without worsening insulin resistance, distinguishing FATP1 from lipotoxic transporters.

    Evidence Muscle-specific Mck/Fatp1 transgenic mice with palmitate transport/oxidation, in vivo BMIPP uptake, and glucose tolerance tests

    PMID:21442160

    Open questions at the time
    • Does not address adipose-specific functions
    • Mechanism directing flux to oxidation vs esterification not defined
  10. 2012 Medium

    Resolved that FATP1 facilitates uptake by metabolic trapping from the ER rather than direct plasma-membrane transport, and refined its retinal phenotype.

    Evidence Retroviral FATP1 overexpression in 3T3-L1 with ER co-localization/fractionation and uptake assays; Fatp1-/- retinal ERG and EM

    PMID:23024797 PMID:23166839

    Open questions at the time
    • Apparent conflict with insulin-driven plasma-membrane translocation not fully reconciled
    • Retinal aging mechanism (vascular vs metabolic) unresolved
  11. 2014 High

    Localized FATP1 specifically to the mitochondrial outer membrane and intermembrane space and showed it channels muscle fatty acids to β-oxidation while sparing ketone bodies.

    Evidence Differential fractionation plus immunogold EM of FATP1-GFP, with in vivo adenoviral overexpression and isolated muscle oxidation assays

    PMID:24858472

    Open questions at the time
    • Mechanism of FATP1 targeting to the outer mitochondrial membrane unknown
    • How soluble IMS pool functions unclear
  12. 2016 High

    Extended FATP1 function to immune cells, showing its acyl-CoA synthetase activity sets macrophage substrate preference and restrains pro-inflammatory activation.

    Evidence Reciprocal Fatp1-/- BMDM and FATP1-overexpressing RAW264.7 with Seahorse bioenergetics, metabolomics, and HFD bone-marrow chimeras

    PMID:27408776

    Open questions at the time
    • Lipid mediators responsible for the inflammatory phenotype not pinpointed
    • Link to the glucose/PDH axis not directly tested
  13. 2017 High

    Demonstrated FATP1 mediates insulin-regulated bidirectional DHA transport at the blood-brain barrier, extending its insulin-translocation behavior to endothelium.

    Evidence FATP1 overexpression/knockdown in HEK293 and hCMEC/D3 with [14C]-DHA uptake/efflux, insulin translocation, and mouse microvessel immunohistochemistry

    PMID:28035674

    Open questions at the time
    • Mechanism of efflux versus influx directionality not defined
    • Whether DHA transport requires synthetase activity untested
  14. 2018 High

    Implicated FATP1 in cancer by showing it imports adipocyte-derived lipids to fuel melanoma growth and is druggable with Lipofermata.

    Evidence Co-culture lipid transfer, BRAFV600E zebrafish melanoma model, mouse xenografts, and Lipofermata inhibition

    PMID:29903879

    Open questions at the time
    • Downstream lipid signaling driving invasion not detailed
    • Selectivity of pharmacologic inhibition for FATP1 not fully addressed
  15. 2019 Medium

    Defined upstream transcriptional control of FATP1 by nuclear receptors — ER-β driving and PPARα/γ plus TNF-α suppressing expression — tying its fatty acid uptake to hormonal and inflammatory cues.

    Evidence ER-β antagonist/estradiol in breast cancer lines and PPAR agonist/TNF-α metabolomics in macrophages, with FATP1 expression and uptake readouts

    PMID:30674874 PMID:31575907

    Open questions at the time
    • Direct transcriptional binding to the SLC27A1 promoter not shown
    • Single-lab pharmacologic interventions
  16. 2022 Medium

    Positioned FATP1 within an adipose browning program downstream of sympathetic Prdm16 signaling.

    Evidence In vivo iWAT siRNA knockdown of Prdm16/FATP1 with sympathetic denervation and energy expenditure measures in Withaferin A-treated mice

    PMID:34732538

    Open questions at the time
    • Mechanistic link between FATP1 fatty acid handling and browning not defined
    • Single lab
  17. 2024 Medium

    Showed FATP1 protein at the BBB is post-translationally downregulated by iron, reducing DHA efflux.

    Evidence Ferric ammonium citrate treatment of hCMEC/D3 with RT-qPCR, Western blot, and [14C]-DHA efflux assays

    PMID:39044044

    Open questions at the time
    • Mechanism of iron-dependent protein turnover unknown
    • Physiological relevance in vivo untested
  18. 2025 Low

    Linked FATP1-mediated LCFA transport to colorectal cancer migration/invasion via DAG-3P synthesis.

    Evidence SLC27A1 knockdown in Caco-2 and T84 cells with migration/invasion and DAG-3P pathway analysis

    PMID:40883416

    Open questions at the time
    • Single loss-of-function experiment without rescue or in vivo validation
    • Mechanistic detail limited

Open questions

Synthesis pass · forward-looking unresolved questions
  • The molecular machinery that reconciles FATP1's intracellular (ER/mitochondrial) localization with its insulin-stimulated plasma-membrane translocation, and the structural basis of its dual transport/synthetase activity, remain unresolved.
  • No structure of FATP1
  • Translocation trafficking partners unidentified
  • Whether moonlighting (RPE65/LRAT) and cancer roles require catalytic activity unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016874 ligase activity 5 GO:0008289 lipid binding 3 GO:0140098 catalytic activity, acting on RNA 3 GO:0005215 transporter activity 2
Localization
GO:0005739 mitochondrion 3 GO:0005783 endoplasmic reticulum 2 GO:0005886 plasma membrane 2
Pathway
R-HSA-1430728 Metabolism 5 R-HSA-162582 Signal Transduction 2 R-HSA-382551 Transport of small molecules 2
Partners
ACSL1CPT1LRATRPE65

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1999 FATP1 (SLC27A1) possesses intrinsic very long chain acyl-CoA synthetase activity, preferentially activating C24:0 over C16:0. Active-site mutants M1 (6-aa substitution at residues 249-254) and M2 (59-aa deletion at residues 464-523) were both catalytically inactive, demonstrating two conserved domains are required for enzymatic function. The proposed mechanism for fatty acid uptake is esterification-coupled influx. Expression of FATP1-Myc/His in COS1 cells, acyl-CoA synthetase activity assays with C24:0 and C16:0 substrates, active-site mutagenesis, nickel-affinity partial purification The Journal of biological chemistry High 10593920
2000 Murine FATP1 functionally complements the yeast fat1Δ deletion, restoring long-chain fatty acid transport (chain-length specific: myristate, palmitate, oleate, but not octanoate), very long chain acyl-CoA synthetase activity (lignoceryl-CoA), and β-oxidation of exogenous fatty acids. This establishes FATP1 and yeast Fat1p as functional orthologues with dual transport and acyl-CoA synthetase activities. Genetic complementation in S. cerevisiae fat1Δ strain; fluorescent fatty acid analogue accumulation assay; radiolabelled fatty acid transport and β-oxidation assays; acyl-CoA synthetase biochemical assay European journal of biochemistry High 10880966
2000 The human FATP1/SLC27A1 gene is organized in 12 exons spanning >13 kb, maps to chromosome 19p13.1 by FISH, and is most highly expressed in muscle and adipose tissue with barely detectable levels in liver. cDNA/genomic cloning, fluorescence in situ hybridization (FISH), Northern blot expression analysis Genomics Medium 10873384
2002 FATP1 stably expressed in 293 cells channels exogenous fatty acids preferentially into triacylglycerol (TG) biosynthesis, associated with increased diacylglycerol acyltransferase activity, while down-regulating de novo sphingomyelin and cholesterol synthesis. FATP1 expression increased both short-term FA uptake and long-term TG incorporation. Stable transfection of 293 cells; radiolabelled fatty acid incorporation assays ([14C]acetate, [3H]oleic acid, [14C]lignoceric acid); BODIPY fatty acid uptake; enzyme activity assays Journal of lipid research Medium 12235169
2005 Among six murine FATP isoforms expressed in yeast, FATP1, -2, and -4 complemented defects in both fatty acid transport and very long chain fatty acid activation (C18:1, C20:4, C24:0). FATP3, -5, and -6 did not complement the transport deficiency despite plasma membrane localization, demonstrating isoform-specific division of transport versus acyl-CoA synthetase functions. Expression of each mmFATP isoform in defined yeast fat1Δ faa1Δ strain; fatty acid transport assays; acyl-CoA synthetase activity assays; subcellular localization The Journal of biological chemistry High 15699031
2005 In cultured human skeletal muscle cells, FATP1 localizes to intracellular membranes in a reticular/perinuclear pattern partly overlapping with Golgi (GM-130 marker), distinct from FAT/CD36 which is on the plasma membrane. Despite its intracellular localization, FATP1 overexpression stimulates palmitate and oleate transport and channels imported fatty acids away from complete oxidation toward triacylglyceride synthesis. Adenoviral gene delivery to primary human myotubes; EGFP fusion protein confocal microscopy; Golgi co-localization; radiolabelled fatty acid transport, oxidation, and esterification assays American journal of physiology. Cell physiology Medium 15897321
2006 FATP1 is an insulin-sensitive fatty acid transporter: in adipocytes and skeletal muscle, FATP1 translocates from an intracellular compartment to the plasma membrane in response to insulin. FATP1-null mice show complete abolition of insulin-stimulated fatty acid uptake in adipocytes and greatly reduced uptake in skeletal muscle, while basal uptake is unaffected. Loss of FATP1 redistributes dietary lipids to the liver and fully protects against diet-induced obesity and insulin resistance. FATP1 knockout mouse model; insulin-stimulated fatty acid uptake assays in adipocytes and skeletal muscle; subcellular fractionation/translocation assays; metabolic phenotyping including serum LCFA, tissue lipid distribution, diet-induced obesity Molecular and cellular biology High 16611988
2009 FATP1 localizes to mitochondria in L6E9 myotubes and skeletal muscle in vivo, and co-immunoprecipitates with CPT1 (carnitine palmitoyltransferase 1). Adenoviral overexpression of FATP1 increases fatty acid oxidation and palmitate esterification. Co-overexpression of FATP1 and CPT1 further enhanced mitochondrial fatty acid oxidation, and all effects were blocked by the CPT1 inhibitor etomoxir, establishing FATP1 as a collaborator with CPT1 for mitochondrial fatty acid import. Adenoviral overexpression in L6E9 myotubes; immunocytochemistry; co-immunoprecipitation from myotubes and rat skeletal muscle; fatty acid oxidation and esterification assays; etomoxir inhibitor experiments Journal of lipid research High 19429947
2009 FATP1 localizes to mitochondria in cultured myotubes (shown by subcellular fractionation immunoblot and FATP1-GFP immunocytology). FATP1 overexpression strongly stimulates CO2 production from glucose and activates the pyruvate dehydrogenase (PDH) complex and its PDH-E1 catalytic subunit, increasing E1β protein content without changing E2, E3BP, or E1α. Subcellular fractionation and immunoblot; FATP1-GFP immunocytology in myotubes; PDH complex activity and subunit protein quantification; CO2 production metabolic assay Mitochondrion Medium 19361580
2009 In 3T3-L1 adipocytes, insulin-stimulated fatty acid influx mediated by FATP1 increases AMP/ATP ratio, which activates AMPK and its downstream target acetyl-CoA carboxylase. Knockdown of FATP1 by shRNA attenuates fatty acid-induced AMPK activation, demonstrating that FATP1-mediated acyl-CoA synthetase activity generates the AMP that signals to AMPK. shRNA knockdown of FATP1 in 3T3-L1 adipocytes; AMPK and ACC phosphorylation by immunoblot; intracellular AMP/ATP ratio measurement; palmitate/arachidonate uptake assays Biochemical and biophysical research communications Medium 19560442
2010 FATP1 mediates AMPK activation by adiponectin and insulin in adipocytes. Knockdown of FATP1 (and ACSL1) abolished the ~2-fold increase in AMP/ATP ratio and AMPK activation induced by adiponectin, and abrogated the 5-fold AMP/ATP increase and AMPK activation by insulin at 40 min. FATP1 and ACSL1 are also required for stimulation of long-chain fatty acid uptake by both hormones. siRNA knockdown of FATP1 and Acsl1 in mouse adipocytes; AMP/ATP ratio measurement; AMPK activation (phospho-AMPK immunoblot); long-chain fatty acid uptake assays FASEB journal Medium 20667975
2010 FATP1 (SLC27A1) interacts with visual cycle proteins RPE65 (retinoid isomerase) and LRAT in the retinal pigment epithelium, identified by yeast two-hybrid screening with full-length human RPE65. The cytosolic C-terminal sequence of FATP1 mediates dose-dependent interaction with native RPE65 and LRAT. FATP1 markedly inhibits 11-cis retinol production by reducing all-trans retinyl ester production and RPE65 isomerase activity. Yeast two-hybrid screen; dose-dependent interaction assays with native RPE65 and LRAT; cellular reconstitution; 11-cis retinol production assay; colocalization in RPE The Journal of biological chemistry Medium 20356843
2011 Muscle-specific overexpression of FATP1 (Mck/Fatp1 transgenic mice) increases palmitate transport by 24% and oxidation by 35% in soleus muscle without altering triacylglycerol esterification or intrinsic mitochondrial oxidation rate. In vivo, FATP1 overexpression does not exacerbate diet-induced insulin resistance or intramuscular triacylglycerol accumulation, channeling LCFA toward oxidation rather than storage. Muscle-specific Fatp1 transgenic mice (Mck promoter); radiolabelled palmitate transport and oxidation assays in isolated soleus; in vivo LCFA uptake (BMIPP scintigraphy); glucose tolerance tests; intramuscular lipid quantification Diabetologia High 21442160
2012 Overexpressed FATP1 localizes to the endoplasmic reticulum (not the plasma membrane) in 3T3-L1 adipocytes as shown by confocal microscopy and subcellular fractionation. Despite intracellular localization, FATP1 overexpression increases acyl-CoA synthetase activity and fatty acid uptake, suggesting that the mechanism of facilitated uptake is metabolic trapping rather than direct plasma membrane transport. Retroviral stable overexpression in 3T3-L1 adipocytes; confocal microscopy co-localization with ER markers; subcellular fractionation; acyl-CoA synthetase activity assay; fatty acid uptake assay; insulin stimulation PloS one Medium 23024797
2012 Fatp1 deficiency in retina leads to decreased electroretinogram response to light and delayed recovery of the b-wave amplitude after bleaching, but without change in visual cycle speed. In 2-year-old Fatp1-/- mice, retinal aging is accelerated with choroid vascularization anomalies, Bruch membrane thickening, and photoreceptor outer segment disorganization. Fatp1-/- mouse model; electroretinography; b-wave bleaching/recovery kinetics; fatty acid pattern analysis; transmission electron microscopy of aged retina PloS one Medium 23166839
2014 FATP1 protein is most abundant in the mitochondrial fraction of mouse gastrocnemius muscle, specifically in outer membrane and soluble intermembrane space subfractions but not in the inner membrane plus matrix. FATP1 overexpression in mouse skeletal muscle reduces circulating fatty acid levels, lowers intramuscular triglyceride content, and enhances palmitate oxidation to CO2 while inhibiting β-hydroxybutyrate oxidation, suggesting FATP1 channels fatty acids to mitochondrial β-oxidation and spares ketone body utilization. Differential centrifugation subcellular fractionation; immunogold electron microscopy of FATP1-GFP in C2C12 myotubes; adenovirus-mediated in vivo overexpression in mouse gastrocnemius; serum metabolite measurements; isolated muscle strip oxidation assays PloS one High 24858472
2016 FATP1 displays acyl-CoA synthetase activity for long-chain fatty acids in macrophages and controls macrophage substrate metabolism. Loss of FATP1 shifts macrophages toward enhanced glucose metabolism and exaggerates pro-inflammatory (classically activated) phenotype. Gain of FATP1 function in RAW 264.7 macrophages decreases glucose metabolism, diminishes pro-inflammatory activation, and modulates lipid mediator metabolism. Fatp1-/- bone marrow-derived macrophage isolation; FATP1-overexpressing RAW 264.7 cell line; bioenergetics assays (Seahorse); metabolomics; inflammatory marker quantification; bone marrow transplant chimeric mouse model with HFD challenge Molecular metabolism High 27408776
2017 FATP1/SLC27A1 at the blood-brain barrier mediates DHA transport: FATP1-expressing HEK293 cells show significantly greater [14C]-DHA uptake than controls; FATP1 knockdown in hCMEC/D3 cells reduces DHA uptake by 59–73%. FATP1 also mediates efflux of DHA, taurine, and biotin. Insulin treatment for 30 min induces translocation of FATP1 to the plasma membrane of brain endothelial cells and enhances DHA uptake. Immunohistochemistry localizes FATP1 to the basal membrane of mouse brain microvessels. FATP1-expressing HEK293 cells; siRNA knockdown in hCMEC/D3 cells; [14C]-DHA uptake and efflux assays; insulin translocation assay; immunohistochemistry of mouse brain sections Journal of neurochemistry High 28035674
2018 Melanomas significantly overexpress FATP1/SLC27A1, which mediates transfer of adipocyte-derived lipids to melanoma cells. FATP1 expression cooperates with BRAFV600E in transgenic zebrafish to accelerate melanoma development. Pharmacologic blockade with Lipofermata (FATP inhibitor) abrogates lipid transport and reduces melanoma growth and invasion. In vitro co-culture models; in vivo zebrafish transgenic melanoma model; mouse xenograft studies; Lipofermata pharmacologic inhibition; lipid transport assays Cancer discovery High 29903879
2019 ER-β (estrogen receptor β) regulates FATP1/SLC27A1 expression in breast cancer cells. Estradiol and fatty acids stimulate FATP1 expression; treatment with ER-β antagonist PHTPP reduces FATP1 expression and inhibits fatty acid uptake and cell viability in four breast cancer cell lines. FATP1 inhibition with arylpiperazine 5k interferes with fatty acid uptake and cell viability. PHTPP (ER-β antagonist) and estradiol treatment; FATP1 expression by qPCR/Western; fatty acid uptake assays; cell viability assays in four breast cancer lines Scientific reports Medium 31575907
2019 PPARα and PPARγ activation by WY14643 and pioglitazone, respectively, attenuates oleate-induced total free fatty acid and triglyceride accumulation in macrophages by suppressing Fatp1 expression, reducing fatty acid influx. TNF-α similarly alleviates oleate-induced lipid accumulation through inhibition of Fatp1. Macrophage treatment with PPAR agonists and TNF-α; untargeted metabolomics; Fatp1 mRNA quantification; fatty acid and triglyceride measurements Cell death & disease Medium 30674874
2022 Withaferin A (WA) induces white adipose tissue browning via a sympathetic nerve-adipose axis requiring the adipocytic Prdm16-FATP1 pathway. WA upregulates Prdm16 and FATP1 (Slc27a1) in inguinal WAT; sympathetic denervation blocks these effects. Prdm16 or FATP1 knockdown in iWAT abrogates WA-induced WAT browning and restores weight gain. Chemical sympathetic denervation; in vivo siRNA knockdown of Prdm16 and FATP1 in iWAT; energy expenditure measurement; Western blot and qPCR; adipose browning markers Diabetes Medium 34732538
2024 Iron (ferric ammonium citrate) reduces FATP1 protein abundance in brain microvascular endothelial cells (hCMEC/D3) by ~20% without affecting mRNA, and this is associated with a ~33% reduction in efflux of [14C]-DHA, demonstrating that FATP1 protein at the BBB mediates DHA efflux and is post-translationally regulated by iron. Ferric ammonium citrate treatment of hCMEC/D3 cells; RT-qPCR and Western blot; [14C]-DHA and [3H]-oleic acid uptake and efflux assays Pharmaceutical research Medium 39044044
2025 SLC27A1 knockdown in colorectal cancer cell lines (Caco-2 and T84) significantly inhibits cell migration and invasion, and the transport of LCFAs via SLC27A1 enhances intracellular synthesis of diacylglycerol-3-phosphate (DAG-3P), a pathway linked to CRC metastasis. SLC27A1 knockdown in CRC cell lines; cell migration and invasion assays; DAG-3P pathway analysis Scientific reports Low 40883416

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2015 The Mitochondrial Permeability Transition Pore: Channel Formation by F-ATP Synthase, Integration in Signal Transduction, and Role in Pathophysiology. Physiological reviews 458 26269524
2018 Adipocyte-Derived Lipids Mediate Melanoma Progression via FATP Proteins. Cancer discovery 308 29903879
2006 FATP1 is an insulin-sensitive fatty acid transporter involved in diet-induced obesity. Molecular and cellular biology 225 16611988
1999 Membrane proteins implicated in long-chain fatty acid uptake by mammalian cells: CD36, FATP and FABPm. Biochimica et biophysica acta 206 10526223
1999 The fatty acid transport protein (FATP1) is a very long chain acyl-CoA synthetase. The Journal of biological chemistry 190 10593920
2017 Ca2+ binding to F-ATP synthase β subunit triggers the mitochondrial permeability transition. EMBO reports 183 28507163
2019 Purified F-ATP synthase forms a Ca2+-dependent high-conductance channel matching the mitochondrial permeability transition pore. Nature communications 157 31554800
2003 Mice with targeted disruption of the fatty acid transport protein 4 (Fatp 4, Slc27a4) gene show features of lethal restrictive dermopathy. The Journal of cell biology 154 12821645
2009 Greater transport efficiencies of the membrane fatty acid transporters FAT/CD36 and FATP4 compared with FABPpm and FATP1 and differential effects on fatty acid esterification and oxidation in rat skeletal muscle. The Journal of biological chemistry 146 19380575
2005 Comparative biochemical studies of the murine fatty acid transport proteins (FATP) expressed in yeast. The Journal of biological chemistry 109 15699031
2016 Metabolic reprogramming through fatty acid transport protein 1 (FATP1) regulates macrophage inflammatory potential and adipose inflammation. Molecular metabolism 106 27408776
2006 Catalytic and mechanical cycles in F-ATP synthases. Fourth in the Cycles Review Series. EMBO reports 103 16607397
2017 The unique histidine in OSCP subunit of F-ATP synthase mediates inhibition of the permeability transition pore by acidic pH. EMBO reports 99 29217657
2009 Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells. Journal of lipid research 94 19429947
2004 Role of FATP in parenchymal cell fatty acid uptake. Biochimica et biophysica acta 83 15522816
2004 The ion channel of F-ATP synthase is the target of toxic organotin compounds. Proceedings of the National Academy of Sciences of the United States of America 75 15277681
2011 Two rotary motors in F-ATP synthase are elastically coupled by a flexible rotor and a stiff stator stalk. Proceedings of the National Academy of Sciences of the United States of America 72 21368147
2012 Overexpressed FATP1, ACSVL4/FATP4 and ACSL1 increase the cellular fatty acid uptake of 3T3-L1 adipocytes but are localized on intracellular membranes. PloS one 71 23024797
2021 Defining the molecular mechanisms of the mitochondrial permeability transition through genetic manipulation of F-ATP synthase. Nature communications 68 34376679
2002 FATP1 channels exogenous FA into 1,2,3-triacyl-sn-glycerol and down-regulates sphingomyelin and cholesterol metabolism in growing 293 cells. Journal of lipid research 68 12235169
2019 PPARα and PPARγ activation attenuates total free fatty acid and triglyceride accumulation in macrophages via the inhibition of Fatp1 expression. Cell death & disease 65 30674874
2010 Activation of AMP-activated protein kinase signaling pathway by adiponectin and insulin in mouse adipocytes: requirement of acyl-CoA synthetases FATP1 and Acsl1 and association with an elevation in AMP/ATP ratio. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 62 20667975
2005 Impact on fatty acid metabolism and differential localization of FATP1 and FAT/CD36 proteins delivered in cultured human muscle cells. American journal of physiology. Cell physiology 61 15897321
2017 Lower Expression of SLC27A1 Enhances Intramuscular Fat Deposition in Chicken via Down-Regulated Fatty Acid Oxidation Mediated by CPT1A. Frontiers in physiology 60 28706492
2007 The fatty acid transport protein (FATP) family: very long chain acyl-CoA synthetases or solute carriers? Journal of molecular neuroscience : MN 60 17901542
2017 The blood-brain barrier fatty acid transport protein 1 (FATP1/SLC27A1) supplies docosahexaenoic acid to the brain, and insulin facilitates transport. Journal of neurochemistry 54 28035674
1999 Fatty acid transporters (FABPpm, FAT, FATP) in human muscle. Canadian journal of applied physiology = Revue canadienne de physiologie appliquee 54 10638339
2016 Bedaquiline Targets the ε Subunit of Mycobacterial F-ATP Synthase. Antimicrobial agents and chemotherapy 49 27620476
2019 TBAJ-876 Retains Bedaquiline's Activity against Subunits c and ε of Mycobacterium tuberculosis F-ATP Synthase. Antimicrobial agents and chemotherapy 47 31358589
2016 Deletion of a unique loop in the mycobacterial F-ATP synthase γ subunit sheds light on its inhibitory role in ATP hydrolysis-driven H(+) pumping. The FEBS journal 47 26996828
2020 The Unique Cysteine of F-ATP Synthase OSCP Subunit Participates in Modulation of the Permeability Transition Pore. Cell reports 45 32877677
2000 The human fatty acid transport protein-1 (SLC27A1; FATP-1) cDNA and gene: organization, chromosomal localization, and expression. Genomics 45 10873384
2020 Structural and functional properties of plant mitochondrial F-ATP synthase. Mitochondrion 43 32534049
2020 Discovery of a Novel Mycobacterial F-ATP Synthase Inhibitor and its Potency in Combination with Diarylquinolines. Angewandte Chemie (International ed. in English) 42 32337801
2018 Physiological and pathological roles of FATP-mediated lipid droplets in Drosophila and mice retina. PLoS genetics 42 30199545
2011 Increasing skeletal muscle fatty acid transport protein 1 (FATP1) targets fatty acids to oxidation and does not predispose mice to diet-induced insulin resistance. Diabetologia 42 21442160
2000 Murine FATP alleviates growth and biochemical deficiencies of yeast fat1Delta strains. European journal of biochemistry 42 10880966
2021 The role of FATP1 in lipid accumulation: a review. Molecular and cellular biochemistry 39 33486652
2018 The NMR solution structure of Mycobacterium tuberculosis F-ATP synthase subunit ε provides new insight into energy coupling inside the rotary engine. The FEBS journal 36 29360236
2006 Expression pattern of fatty acid transport protein-1 (FATP-1), FATP-4 and heart-fatty acid binding protein (H-FABP) genes in human term placenta. Early human development 36 16574350
2022 The mitochondrial chaperone TRAP1 regulates F-ATP synthase channel formation. Cell death and differentiation 35 35614131
2017 The uniqueness of subunit α of mycobacterial F-ATP synthases: An evolutionary variant for niche adaptation. The Journal of biological chemistry 35 28495884
2005 Structural evidence for a constant c11 ring stoichiometry in the sodium F-ATP synthase. The FEBS journal 32 16262688
2016 Neonatal phthalate ester exposure induced placental MTs, FATP1 and HFABP mRNA expression in two districts of southeast China. Scientific reports 31 26867681
2019 Disrupting coupling within mycobacterial F-ATP synthases subunit ε causes dysregulated energy production and cell wall biosynthesis. Scientific reports 30 31727946
2019 Mitochondrial F-ATP Synthase and Its Transition into an Energy-Dissipating Molecular Machine. Oxidative medicine and cellular longevity 28 31178976
2015 The molecular motor F-ATP synthase is targeted by the tumoricidal protein HAMLET. Journal of molecular biology 28 25681694
2012 Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris). The Journal of experimental biology 28 22723485
2019 Unraveling FATP1, regulated by ER-β, as a targeted breast cancer innovative therapy. Scientific reports 26 31575907
2014 Fatty acid transport protein 1 (FATP1) localizes in mitochondria in mouse skeletal muscle and regulates lipid and ketone body disposal. PloS one 26 24858472
2017 The blood-brain barrier and protein-mediated fatty acid uptake: role of the blood-brain barrier as a metabolic barrier: An Editorial Comment for 'The blood-brain barrier fatty acid transport protein 1 (FATP1/SLC27A1) supplies docosahexaenoic acid to the brain, and insulin facilitates transport'. Journal of neurochemistry 24 28369884
2019 Unique structural and mechanistic properties of mycobacterial F-ATP synthases: Implications for drug design. Progress in biophysics and molecular biology 23 31743686
2015 Danqi Pill regulates lipid metabolism disorder induced by myocardial ischemia through FATP-CPTI pathway. BMC complementary and alternative medicine 21 25885422
2006 Structural and functional characterization of the bovine solute carrier family 27 member 1 (SLC27A1) gene. Cytogenetic and genome research 21 17065791
2022 Withaferin A Promotes White Adipose Browning and Prevents Obesity Through Sympathetic Nerve-Activated Prdm16-FATP1 Axis. Diabetes 19 34732538
2013 Arylpiperazines as fatty acid transport protein 1 (FATP1) inhibitors with improved potency and pharmacokinetic properties. Bioorganic & medicinal chemistry letters 19 23528296
2022 Binding properties of the anti-TB drugs bedaquiline and TBAJ-876 to a mycobacterial F-ATP synthase. Current research in structural biology 18 36186842
2012 Solution structure of subunit γ (γ(1-204)) of the Mycobacterium tuberculosis F-ATP synthase and the unique loop of γ(165-178), representing a novel TB drug target. Journal of bioenergetics and biomembranes 18 23104121
2020 The Unique C-Terminal Extension of Mycobacterial F-ATP Synthase Subunit α Is the Major Contributor to Its Latent ATP Hydrolysis Activity. Antimicrobial agents and chemotherapy 17 32988828
2010 Cloning and functional characterization of a fatty acid transport protein (FATP) from the pheromone gland of a lichen moth, Eilema japonica, which secretes an alkenyl sex pheromone. Insect biochemistry and molecular biology 17 20875854
2009 FATP1 localizes to mitochondria and enhances pyruvate dehydrogenase activity in skeletal myotubes. Mitochondrion 17 19361580
2019 Structural Asymmetry and Kinetic Limping of Single Rotary F-ATP Synthases. Molecules (Basel, Switzerland) 16 30704145
2012 FATP1 silence inhibits the differentiation and induces the apoptosis in chicken preadipocytes. Molecular biology reports 16 23187737
2009 FATP1 mediates fatty acid-induced activation of AMPK in 3T3-L1 adipocytes. Biochemical and biophysical research communications 16 19560442
2010 FATP1 inhibits 11-cis retinol formation via interaction with the visual cycle retinoid isomerase RPE65 and lecithin:retinol acyltransferase. The Journal of biological chemistry 15 20356843
2008 [The role of fatty-acid transport proteins (FAT/CD36, FABPpm, FATP) in lipid metabolism in skeletal muscles]. Postepy higieny i medycyny doswiadczalnej (Online) 15 18772848
2022 Mutational Analysis of Mycobacterial F-ATP Synthase Subunit δ Leads to a Potent δ Enzyme Inhibitor. ACS chemical biology 14 35148057
2018 Structure and function of Mycobacterium-specific components of F-ATP synthase subunits α and ε. Journal of structural biology 14 30342092
2012 Fatp1 deficiency affects retinal light response and dark adaptation, and induces age-related alterations. PloS one 14 23166839
2017 Lack of myeloid Fatp1 increases atherosclerotic lesion size in Ldlr-/- mice. Atherosclerosis 13 29035781
2022 Anti-Mycobacterium abscessus Activity of Tuberculosis F-ATP Synthase Inhibitor GaMF1. Antimicrobial agents and chemotherapy 11 35481752
2022 Atomic solution structure of Mycobacterium abscessus F-ATP synthase subunit ε and identification of Ep1MabF1 as a targeted inhibitor. The FEBS journal 11 35612822
2021 Modulation and Pharmacology of the Mitochondrial Permeability Transition: A Journey from F-ATP Synthase to ANT. Molecules (Basel, Switzerland) 11 34770872
2024 High efficacy of the F-ATP synthase inhibitor TBAJ-5307 against nontuberculous mycobacteria in vitro and in vivo. The Journal of biological chemistry 10 38176652
2018 Expression of APOB, ADFP and FATP1 and their correlation with fat deposition in Yunnan's top six famous chicken breeds. British poultry science 10 30004246
2021 The Effect of FATP1 on Adipocyte Differentiation in Qinchuan Beef Cattle. Animals : an open access journal from MDPI 9 34679811
2012 Discovery and optimization of novel fatty acid transport protein 1 (FATP1) inhibitors. Bioorganic & medicinal chemistry letters 9 22749869
2025 SQ31f is a potent non-tuberculous mycobacteria antibiotic by specifically targeting the mycobacterial F-ATP synthase. The Journal of antimicrobial chemotherapy 8 39499211
2019 Purification of Functional F-ATP Synthase from Blue Native PAGE. Methods in molecular biology (Clifton, N.J.) 7 30674031
2019 Overexpression, purification, enzymatic and microscopic characterization of recombinant mycobacterial F-ATP synthase. Biochemical and biophysical research communications 7 31761325
2024 Human F-ATP synthase as a drug target. Pharmacological research 6 39303772
2024 N-terminal cleavage of cyclophilin D boosts its ability to bind F-ATP synthase. Communications biology 6 39528709
2022 The SLC27A1 Gene and Its Enriched PPAR Pathway Are Involved in the Regulation of Flavor Compound Hexanal Content in Chinese Native Chickens. Genes 6 35205238
2019 The structural features of Acetobacterium woodii F-ATP synthase reveal the importance of the unique subunit γ-loop in Na+ translocation and ATP synthesis. The FEBS journal 6 30791207
2015 Stoichiometry and deletion analyses of subunits in the heterotrimeric F-ATP synthase c ring from the acetogenic bacterium Acetobacterium woodii. The FEBS journal 6 26613566
2013 Two-dimensional crystallization of intact F-ATP synthase isolated from bovine heart mitochondria. Acta crystallographica. Section F, Structural biology and crystallization communications 6 24316832
2010 Association of FATP1 gene polymorphisms with chicken carcass traits in Chinese meat-type quality chicken populations. Molecular biology reports 6 20204526
2023 F-ATP synthase inhibitory factor 1 regulates metabolic reprogramming involving its interaction with c-Myc and PGC1α. Frontiers in oncology 5 37469400
2023 Mitochondrial F-ATP Synthase Co-Migrating Proteins and Ca2+-Dependent Formation of Large Channels. Cells 5 37830628
2011 Association between polymorphisms in the SLC27A1 gene and milk production traits in Chinese Holstein cattle. Animal biotechnology 5 21328100
2024 F-ATP synthase inhibitory factor 1 and mitochondria-organelle interactions: New insight and implications. Pharmacological research 4 39233058
2022 Single-molecule FRET combined with electrokinetic trapping reveals real-time enzyme kinetics of individual F-ATP synthases. Nanoscale 4 35084006
2025 Water occupancy in the Acinetobacter baumannii F-ATP synthase c-ring and its implications as a novel inhibitor target. Structure (London, England : 1993) 3 39892380
2024 Iron Reduces the Trafficking of Fatty Acids from Human Immortalised Brain Microvascular Endothelial Cells Through Modulation of Fatty Acid Transport Protein 1 (FATP1/SLC27A1). Pharmaceutical research 3 39044044
2024 Rice Protein Reduces Triglyceride Levels through Modulating CD36, MTP, FATP, and FABP Expression in Growing and Adult Rats. Foods (Basel, Switzerland) 3 39272469
2023 Large-scale column-free purification of bovine F-ATP synthase. The Journal of biological chemistry 3 38159856
2022 The involvement of FATP1 regulating skeletal muscle fat deposition in stressed broilers was affected by fatty acid substrates. Frontiers in veterinary science 3 35909684
2015 Genetic polymorphisms of the FATP1 gene and their associations with meat quality traits in Chinese Qinchuan cattle. Genetics and molecular research : GMR 3 26782386
2025 Long chain fatty acid transport via SLC27A1 enhances DAG-3-P synthesis and accelerates colorectal cancer metastasis. Scientific reports 2 40883416
2024 Identification and Functional Characterization of the FATP1 Gene from Mud Crab, Scylla paramamosain. Animals : an open access journal from MDPI 1 39457899

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