| 2016 |
Sema3d regulates collective endothelial cell migration in zebrafish through two distinct mechanisms: (1) mesenchymal Sema3d guides outgrowth of the common cardinal vein via repulsion signaling through PlexinD1, and (2) autocrine Sema3d signaling within endothelial cells regulates actin network organization and cell morphology through Neuropilin1, which then signals via RhoA upstream of Rock to stabilize the endothelial sheet. |
Zebrafish genetic/knockdown experiments with receptor-specific dissection, live imaging of endothelial cell migration, and pathway manipulation (RhoA/Rock inhibition) |
The Journal of cell biology |
High |
27799363
|
| 2016 |
Sema3d is stabilized by the Hapln1a-dependent extracellular matrix (ECM) during zebrafish fin regeneration; Hapln1a knockdown reduces hyaluronan and aggrecan, destabilizing Sema3d, and Sema3d overexpression rescues hapln1a knockdown phenotypes, placing Sema3d functionally downstream of the Hapln1a-ECM in Cx43-dependent skeletal patterning and growth. |
Double morpholino knockdown, rescue by Sema3d overexpression, epistasis analysis in zebrafish fin regeneration |
PloS one |
Medium |
26828861
|
| 2022 |
Sema3d interacts with the actin-binding protein Filamin A (FLNA) as identified by co-immunoprecipitation combined with mass spectrometry, and this interaction inactivates the PI3K/Akt signaling pathway and remodels the cytoskeleton to suppress hepatocellular carcinoma proliferation, invasion, and metastasis. |
Co-IP combined with mass spectrometry, RNA sequencing with GSEA, gain- and loss-of-function experiments in HCC cell lines and mouse xenograft models |
Frontiers in oncology |
Medium |
35957887
|
| 2026 |
Sema3d secreted by activated fibroblasts signals through the endothelial Plexin D1 receptor, leading to Arf6 activation and integrin β1 internalization, thereby suppressing focal adhesion formation, inducing cytoskeletal collapse, and preventing endothelial cell migration and angiogenesis, which promotes renal fibrosis in the AKI-CKD transition. |
Single-cell RNA sequencing, conditioned medium experiments on HUVECs, mechanistic pathway analysis (Arf6/integrin β1), and AAV9-shRNA knockdown of Sema3d in mouse uIRI model |
International journal of biological sciences |
Medium |
41608631
|
| 2025 |
Citrullination of IGF2BP1 at R167 promotes the mRNA stability of SEMA3D by enhancing the interaction between IGF2BP1 and its cofactor ELAVL1, thereby upregulating SEMA3D expression and promoting proliferation, migration, and invasion of rheumatoid arthritis fibroblast-like synoviocytes. |
Transcriptomic sequencing, functional assays in RA-FLSs with citrullination site-specific mutagenesis (R167K knock-in mice), co-IP to detect IGF2BP1-ELAVL1 interaction, mRNA stability assays |
Communications biology |
Medium |
39809921
|
| 2014 |
Disruption of SEMA3D (partial tandem duplication producing a truncated mRNA) in a patient with congenital heart defects suggests that Sema3d is required for correct migration of cardiac neural crest cells (CNCC) into the outflow tract; Sema3d-null mice present with congenital heart defects consistent with CNCC migration failure. |
Array comparative genomic hybridization, breakpoint sequencing, fiber FISH, expression analysis in patient lymphoblasts, reference to Sema3D knockout mouse phenotype |
Human mutation |
Low |
25230848
|
| 2017 |
Genetic epistasis testing in mice showed that loss of Sema3d (null homozygotes) did not affect survival, myenteric plexus formation, or intestinal transcriptome, and there was no evidence of genetic interaction between Ret and Sema3d on these phenotypes — a negative finding for Sema3d's role in enteric nervous system development in this context. |
Double-null intercross mouse genetics, acetylcholinesterase staining for myenteric plexus, RNA-sequencing of embryonic intestine |
Human molecular genetics |
Medium |
28334784
|
| 2009 |
In the developing rat dorsal root ganglia, Sema3D expression is confined to proprioceptive neurons that co-express TrkC and Runx3, but not to nociceptive TrkA-expressing neurons, suggesting a selective role for Sema3D in axonal navigation of large-diameter proprioceptive DRG neurons. |
In situ hybridization and double immunohistochemical analysis of rat embryonic DRG with TrkA, TrkC, Runx3, and Neuropilin-1 markers |
Neuroscience letters |
Low |
19429098
|
| 2024 |
SEMA3D protein is detected as extracellular granules in the tongue primordia at E11.5, while NRP1 is expressed in the hypoglossal nerve; SEMA3D granules are excluded from regions where hypoglossal nerve axons elongate, consistent with a repulsive guidance role for SEMA3D on NRP1-expressing hypoglossal axons during tongue development. |
Transcriptomic ROKU analysis, quantitative PCR, and immunohistochemistry on mouse embryonic tongue primordia |
Acta histochemica et cytochemica |
Low |
38463205
|