SEC61B
Protein transport protein Sec61 subunit beta · UniProt P60468
Round 2 corrected
Audit flag: rerun needed
- Length
- 96 aa
- Mass
- 10.0 kDa
- Annotated
- 2026-04-28
65 papers in source corpus
14 papers cited in narrative
13 extracted findings
Mechanistic narrative
Synthesis pass · prose summary of the discoveries below
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Mechanism profile
Synthesis pass · controlled-vocabulary classification · explore literature graph →
No controlled-vocabulary terms were assigned to this entry.
Evidence
Reading pass · 13 per-paper findings extracted from the source corpus
| Year | Finding | Method | Journal | Conf | PMIDs |
|---|---|---|---|---|---|
| 1994 | SEC61B (Sec61-beta) is a subunit of the mammalian Sec61p complex, the core protein translocation channel of the endoplasmic reticulum. Its gamma subunit (Sec61-gamma) is homologous to yeast SSS1p and can functionally replace it in yeast, establishing evolutionary conservation of the translocon complex across eukaryotes and bacteria. | Sequence determination, functional complementation in yeast, structural homology analysis | Nature | High | 8107851 |
| 1996 | The Sec61 complex (including SEC61B as a subunit) mediates retrotranslocation of MHC class I molecules from the ER lumen to the cytosol for proteasomal degradation, a process hijacked by the HCMV US2 protein, demonstrating that the translocon can operate in reverse for ERAD. | Biochemical fractionation, immunoprecipitation, proteasome inhibition, cell biology | Nature | High | 8945469 |
| 2000 | Mammalian SEC61B is part of a ribosome-free Sec61 complex that associates with mammalian SEC62 and SEC63, forming a higher-order complex at the ER membrane analogous to the yeast post-translational translocation machinery. | Biochemical fractionation, co-immunoprecipitation, sequence analysis | The Journal of biological chemistry | High | 10799540 |
| 2015 | SEC61B mRNA is a tail-anchored protein whose transcript is partially localized to the ER surface in mammalian cells via both translation-dependent and translation-independent mechanisms, independent of p180/RRBP1 and TRC40/BAT3 pathway components, suggesting that Sec61β is synthesized directly on the ER to facilitate membrane insertion. | Fluorescence in situ hybridization (FISH), live-cell imaging, siRNA knockdown, cell fractionation | Journal of cell science | Medium | 26272916 |
| 2017 | Loss-of-function of SEC61B in cell line models results in defective maturation and trafficking of polycystin-1, placing SEC61B in the ER protein biogenesis pathway as a regulator of polycystin-1 processing. SEC61B loss-of-function has distinct effects on polycystin-1 biogenesis compared to loss of other PCLD genes (PRKCSH, SEC63, ALG8, GANAB). | Gene inactivation in cell lines, polycystin-1 maturation/trafficking assays, whole exome sequencing | The Journal of clinical investigation | Medium | 28375157 28862642 |
| 2017 | Endogenous SEC61B protein has reduced abundance in certain peripheral ER tubules compared to the ER sheet network, as revealed by dual-color endogenous tagging with split fluorescent proteins (GFP11/sfCherry211), demonstrating non-uniform distribution within ER subdomains. | CRISPR endogenous tagging with split fluorescent proteins, super-resolution and confocal live-cell imaging | Nature communications | Medium | 28851864 |
| 2017 | SEC61B was identified as part of the SEC63 interactome by affinity proteomics, confirming that SEC61B is a component of the SEC63-containing translocon complex at the ER membrane in cholangiocytes. | Affinity proteomics (immunoprecipitation-MS) in HEK293T cells and H69 cholangiocytes | Human molecular genetics | Medium | 28973524 |
| 2019 | DDR1 nuclear translocation requires its interaction with SEC61B (a component of the Sec61 translocon), as well as nonmuscle myosin IIA and β-actin. This interaction enables collagen-activated DDR1 to move from the ER/membrane to the nucleus where it drives collagen IV transcription. | Co-immunoprecipitation, biochemical fractionation, immunofluorescence, mutant DDR1 constructs | Journal of the American Society of Nephrology : JASN | Medium | 31383731 |
| 2021 | SEC61B anchored to the ER lumen (as SEC61b-TurboID) selectively labels proteins that transit through the classical secretory pathway via proximity biotinylation, validating that SEC61B resides in the ER translocon and that transiting secretory proteins pass through its proximity. | Proximity labeling (TurboID), in vivo mouse model, quantitative proteomics | Nature communications | High | 34471136 |
| 2021 | SEC61B expression is decreased in dendritic cells following LPS-induced reticulophagy (FAM134B-mediated), consistent with SEC61B being selectively degraded during ER-phagy of ER membrane subdomains. | Western blotting, FAM134B siRNA knockdown, LPS stimulation time course | Zhonghua shao shang yu chuang mian xiu fu za zhi | Low | 37805802 |
| 2021 | Let-7b-5p upregulates SERP1 (stress-associated ER protein 1), which modulates its chaperone protein SEC61B during ER stress response, activating the unfolded protein response via PERK-CHOP pathway. | miRNA mimic/inhibitor transfection, qPCR, Western blot, in vitro and in vivo PE models | IUBMB life | Low | 32534478 |
| 2025 | SEC61B overexpression in HEK293 cells increases calcium flux from the ER into the cytosol and decreases protein synthesis. In hyperglycemic mice and humans with diabetes, platelet SEC61B is elevated, ER stress induces SEC61B expression, and increased SEC61B causes elevated cytosolic calcium leading to platelet hyperreactivity. Pharmacological inhibition of Sec61 with anisomycin decreased platelet calcium flux and inhibited platelet aggregation in vitro and in vivo. | HEK293 overexpression calcium flux assays, proteomic profiling of human/mouse platelets, pharmacological inhibition (anisomycin), in vivo thrombosis models | The Journal of clinical investigation | High | 40829182 |
| 2025 | SEC61B is required for proteolytic cleavage (by furin and furin-related enzyme SKI-1) and normal N-glycosylation of Marburg virus glycoprotein (MARV-GP) but not Ebola virus GP, revealing a virus-specific role of SEC61B in MARV-GP processing. SEC61B KO impaired SKI-1-mediated cleavage of MARV-GP and abrogated robust MARV cell entry. Blockade of the Sec61 translocon with apratoxin S4 markedly inhibited both EBOV and MARV infection. | SEC61B CRISPR knockout cells, pseudovirus entry assays, Western blot for GP cleavage and glycosylation, in silico cleavage motif identification | bioRxivpreprint | Medium | bio_10.1101_2025.06.26.660697 |
Source papers
Stage 0 corpus · 65 papers · ranked by NIH iCite citations
| Year | Title | Journal | Citations | PMID |
|---|---|---|---|---|
| 2006 | Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. | Cell | 2861 | 17081983 |
| 2012 | Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. | Cell | 1718 | 22658674 |
| 2002 | Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. | Proceedings of the National Academy of Sciences of the United States of America | 1479 | 12477932 |
| 2015 | A human interactome in three quantitative dimensions organized by stoichiometries and abundances. | Cell | 1015 | 26496610 |
| 1996 | Sec61-mediated transfer of a membrane protein from the endoplasmic reticulum to the proteasome for destruction. | Nature | 911 | 8945469 |
| 2021 | Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. | Cell | 705 | 33961781 |
| 2011 | Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. | Briefings in bioinformatics | 656 | 21873635 |
| 2018 | High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies. | Molecular cell | 580 | 29395067 |
| 2021 | Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. | Nature | 532 | 33845483 |
| 1994 | Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. | Gene | 492 | 8125298 |
| 2004 | The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). | Genome research | 438 | 15489334 |
| 2022 | OpenCell: Endogenous tagging for the cartography of human cellular organization. | Science (New York, N.Y.) | 432 | 35271311 |
| 2015 | Panorama of ancient metazoan macromolecular complexes. | Nature | 407 | 26344197 |
| 1998 | The role of assembly in insulin's biosynthesis. | Current opinion in structural biology | 406 | 9631292 |
| 2021 | A proximity-dependent biotinylation map of a human cell. | Nature | 339 | 34079125 |
| 2012 | Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins. | Nature | 319 | 22810586 |
| 2019 | Intrinsically Disordered Protein TEX264 Mediates ER-phagy. | Molecular cell | 296 | 31006538 |
| 2020 | The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis. | Nature | 292 | 32322062 |
| 2017 | Genetic Complexity of Autosomal Dominant Polycystic Kidney and Liver Diseases. | Journal of the American Society of Nephrology : JASN | 273 | 29038287 |
| 2022 | Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration. | Cell | 256 | 35063084 |
| 1994 | Evolutionary conservation of components of the protein translocation complex. | Nature | 241 | 8107851 |
| 2010 | A ribosome-associating factor chaperones tail-anchored membrane proteins. | Nature | 238 | 20676083 |
| 2018 | Prevalence Estimates of Polycystic Kidney and Liver Disease by Population Sequencing. | Journal of the American Society of Nephrology : JASN | 218 | 30135240 |
| 2014 | Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes. | Journal of proteomics | 215 | 25281560 |
| 2016 | Ubiquilins Chaperone and Triage Mitochondrial Membrane Proteins for Degradation. | Molecular cell | 210 | 27345149 |
| 2009 | Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. | Science signaling | 207 | 19738201 |
| 2017 | Improved split fluorescent proteins for endogenous protein labeling. | Nature communications | 197 | 28851864 |
| 2020 | Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions. | Nature cell biology | 194 | 32203420 |
| 2019 | H4K20me0 recognition by BRCA1-BARD1 directs homologous recombination to sister chromatids. | Nature cell biology | 162 | 30804502 |
| 2019 | A protein-interaction network of interferon-stimulated genes extends the innate immune system landscape. | Nature immunology | 159 | 30833792 |
| 2000 | Mammalian Sec61 is associated with Sec62 and Sec63. | The Journal of biological chemistry | 158 | 10799540 |
| 2009 | The structural basis of tail-anchored membrane protein recognition by Get3. | Nature | 155 | 19675567 |
| 2020 | AMPK, a Regulator of Metabolism and Autophagy, Is Activated by Lysosomal Damage via a Novel Galectin-Directed Ubiquitin Signal Transduction System. | Molecular cell | 152 | 31995728 |
| 2017 | Isolated polycystic liver disease genes define effectors of polycystin-1 function. | The Journal of clinical investigation | 127 | 28375157 |
| 2020 | Insights into Autosomal Dominant Polycystic Kidney Disease from Genetic Studies. | Clinical journal of the American Society of Nephrology : CJASN | 101 | 32690722 |
| 2021 | Dynamic tracking and identification of tissue-specific secretory proteins in the circulation of live mice. | Nature communications | 90 | 34471136 |
| 2014 | Proteomic analysis of solid pseudopapillary tumor of the pancreas reveals dysfunction of the endoplasmic reticulum protein processing pathway. | Molecular & cellular proteomics : MCP | 86 | 24997997 |
| 2019 | WDR45 contributes to neurodegeneration through regulation of ER homeostasis and neuronal death. | Autophagy | 81 | 31204559 |
| 2022 | Exome Sequencing of a Clinical Population for Autosomal Dominant Polycystic Kidney Disease. | JAMA | 71 | 36573973 |
| 2022 | Human T-bet governs the generation of a distinct subset of CD11chighCD21low B cells. | Science immunology | 65 | 35867801 |
| 2015 | Genetic ablation of ataxin-2 increases several global translation factors in their transcript abundance but decreases translation rate. | Neurogenetics | 58 | 25721894 |
| 2018 | A combination of curcumin and oligomeric proanthocyanidins offer superior anti-tumorigenic properties in colorectal cancer. | Scientific reports | 54 | 30218018 |
| 2022 | Upregulation of ribosome complexes at the blood-brain barrier in Alzheimer's disease patients. | Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism | 53 | 35766008 |
| 2019 | The Extracellular Matrix Receptor Discoidin Domain Receptor 1 Regulates Collagen Transcription by Translocating to the Nucleus. | Journal of the American Society of Nephrology : JASN | 41 | 31383731 |
| 2017 | Isolated polycystic liver disease genes define effectors of polycystin-1 function. | The Journal of clinical investigation | 35 | 28862642 |
| 2024 | USP20 deubiquitinates and stabilizes the reticulophagy receptor RETREG1/FAM134B to drive reticulophagy. | Autophagy | 29 | 38705724 |
| 2017 | 3,4-dihydroxybenzalacetone and caffeic acid phenethyl ester induce preconditioning ER stress and autophagy in SH-SY5Y cells. | Journal of cellular physiology | 28 | 28681934 |
| 2011 | Identification of candidate genes linking systemic inflammation to atherosclerosis; results of a human in vivo LPS infusion study. | BMC medical genomics | 25 | 21827714 |
| 2019 | Genetics of polycystic liver diseases. | Current opinion in gastroenterology | 24 | 30652979 |
| 2015 | mRNA encoding Sec61β, a tail-anchored protein, is localized on the endoplasmic reticulum. | Journal of cell science | 18 | 26272916 |
| 2020 | Let-7b-5p is involved in the response of endoplasmic reticulum stress in acute pulmonary embolism through upregulating the expression of stress-associated endoplasmic reticulum protein 1. | IUBMB life | 13 | 32534478 |
| 2017 | Liver cyst gene knockout in cholangiocytes inhibits cilium formation and Wnt signaling. | Human molecular genetics | 11 | 28973524 |
| 2024 | Clinical manifestation, epidemiology, genetic basis, potential molecular targets, and current treatment of polycystic liver disease. | Orphanet journal of rare diseases | 9 | 38671465 |
| 2021 | Autophagy-mediated reduction of miR-345 contributes to hepatic cystogenesis in polycystic liver disease. | JHEP reports : innovation in hepatology | 8 | 34568801 |
| 2025 | SEC61B regulates calcium flux and platelet hyperreactivity in diabetes. | The Journal of clinical investigation | 6 | 40829182 |
| 2020 | cDNA expression library screening revealed novel functional genes involved in clear cell carcinogenesis of the ovary in vitro. | Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology | 4 | 32157937 |
| 2024 | Identification of novel endoplasmic reticulum-related genes and their association with immune cell infiltration in major depressive disorder. | Journal of affective disorders | 3 | 38604455 |
| 2025 | The Mechanism of Xuanyu Tongjing Decoction Regulating NOD/NFκB Pathway to Inhibit Ectopic Tissue Inflammation to Reduce Ovarian Damage in Rats with Ovarian Endometriosis. | Drug design, development and therapy | 2 | 40231194 |
| 2025 | Autosomal Dominant Polycystic Kidney Disease-Related Multifocal Renal Cell Carcinoma: A Narrative Iconographic Review. | International journal of molecular sciences | 2 | 40362206 |
| 2025 | Chronic diseases alter the platelet rheostat to promote hyperreactivity and thrombosis. | The Journal of clinical investigation | 2 | 40829178 |
| 2024 | Identification of differentially expressed ER stress-related genes and their association with pulmonary arterial hypertension. | Respiratory research | 2 | 38789967 |
| 2022 | Comparative proteomics analysis of human stem cells from dental gingival and periodontal ligament. | Proteomics | 2 | 35297194 |
| 2023 | [Influence of family with sequence similarity 134, member B-mediated reticulophagy on lipopolysaccharide-induced apoptosis of mouse dendritic cells]. | Zhonghua shao shang yu chuang mian xiu fu za zhi | 1 | 37805802 |
| 2025 | ER stress-driven unfolded protein response fuels aging-related tumor aggressiveness in gliomas. | Frontiers in molecular biosciences | 0 | 40718795 |
| 2025 | TIPE2-Regulated ER-Phagy in Dendritic Cell Function Following Hemorrhagic Shock. | Shock (Augusta, Ga.) | 0 | 41697115 |