Affinage

SEC61B

Protein transport protein Sec61 subunit beta · UniProt P60468

Audit flag: model fault
Length
96 aa
Mass
10.0 kDa
Annotated
2026-06-13
36 papers in source corpus 11 papers cited in narrative 10 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

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Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
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Evidence

Reading pass · 10 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2017 SEC61B loss-of-function results in defective maturation and trafficking of polycystin-1 (PC1), the central determinant of cyst pathogenesis in polycystic liver disease. Cell line knockout of SEC61B demonstrated distinct effects on PC1 biogenesis compared to other PCLD gene knockouts, placing SEC61B in the ER protein biogenesis pathway upstream of PC1 maturation. CRISPR/siRNA-based gene inactivation in cell line models with functional readout of polycystin-1 maturation and trafficking The Journal of clinical investigation High 28375157 28862642
2015 Sec61b mRNA is partially localized to the surface of the ER in mammalian cells via both translation-dependent and translation-independent mechanisms, facilitating co-translational insertion of the Sec61β tail-anchored protein directly into the ER membrane. This localization was found to be independent of p180 (RRBP1) and TRC pathway components TRC40 and BAT3. Fluorescence microscopy (live imaging and FISH), subcellular fractionation, and dominant-negative/knockdown experiments for TRC40, BAT3, and p180 Journal of cell science Medium 26272916
2017 Super-resolution imaging of endogenously tagged SEC61B (using split sfCherry2) revealed that SEC61B has reduced abundance in certain peripheral ER tubules compared to the ER sheet network, establishing a non-uniform subcellular distribution within the ER. Dual-color endogenous protein tagging with split fluorescent proteins (sfCherry211 and GFP11) and super-resolution microscopy Nature communications Medium 28851864
2019 SEC61B, as a component of the Sec61 translocon, was identified as a binding partner of SEC63 by affinity proteomics (PLD interactome mapping), confirming its physical association with SEC63 within the ER translocon complex in cholangiocytes. Affinity proteomics (AP-MS) in HEK293T cells and H69 cholangiocytes Human molecular genetics Medium 28973524
2019 DDR1 nuclear translocation requires interaction with SEC61B (a component of the Sec61 translocon), nonmuscle myosin IIA, and β-actin. Collagen-activated DDR1 uses SEC61B as part of the nuclear translocation machinery to regulate collagen IV transcription. Biochemical assays and immunofluorescence with co-immunoprecipitation of DDR1 with SEC61B; loss-of-function experiments Journal of the American Society of Nephrology : JASN Medium 31383731
2021 Sec61b-TurboID fusion (ER lumen-anchored) efficiently labels proteins transiting through the classical secretory pathway in vivo, demonstrating that SEC61B resides in the ER lumen-facing portion of the translocon and can serve as an anchor for proximity labeling of ER-transiting secretory proteins. In vivo proximity labeling (TurboID) anchored via Sec61b in mouse liver, validated by proteomics of labeled plasma proteins Nature communications Medium 34471136
2025 SEC61B overexpression in HEK293 cells increased calcium flux from the ER into the cytosol and decreased protein synthesis, consistent with SEC61B acting as an ER calcium leak channel. In hyperglycemic mice and diabetic humans, elevated platelet SEC61B was associated with increased cytosolic calcium and platelet hyperreactivity. ER stress induced SEC61B expression, and inhibition of Sec61 with anisomycin decreased platelet calcium flux and inhibited platelet aggregation in vitro and in vivo. SEC61B overexpression in HEK293 cells with calcium flux measurement; hyperglycemic mouse models; pharmacological inhibition of Sec61 (anisomycin) in vitro and in vivo; proteomic profiling of human and mouse platelets The Journal of clinical investigation High 40829182
2025 SEC61B (Sec61β subunit) is required for proteolytic cleavage and normal N-glycosylation of Marburg virus (MARV) glycoprotein, but is dispensable for MARV-GP expression itself. In SEC61B-KO cells, SKI-1-mediated cleavage of MARV-GP was impaired. Loss of SEC61B modestly inhibited both EBOV and MARV infection, while pharmacological Sec61 blockade with apratoxin S4 more markedly inhibited infection. SEC61B knockout cells (CRISPR), glycoprotein cleavage assays, N-glycosylation analysis, viral entry assays, pharmacological inhibition with apratoxin S4, in silico identification of SKI-1 cleavage motif bioRxivpreprint Medium bio_10.1101_2025.06.26.660697
2020 SERP1 (stress-associated ER protein 1) activation modulates its chaperone protein SEC61B during ER stress responses, placing SEC61B downstream of SERP1 in the ER stress pathway in lung epithelial cells. miRNA mimic/inhibitor transfection with Western blotting for SERP1 and SEC61B; in vitro and in vivo acute pulmonary embolism models IUBMB life Low 32534478
2026 SEC61B is recruited to stress granules along with calnexin (CANX) under ER stress conditions; inhibition of SG formation by Micranthin B prevented this SEC61B/CANX recruitment, reduced accumulation of misfolded proteins, and alleviated ER stress in hepatocytes. Drug treatment (Micranthin B) in HFHC diet mouse model and PA-stimulated hepatocytes; Western blotting and immunofluorescence for SEC61B and SG markers Chinese journal of natural medicines Low 42215158

Source papers

Stage 0 corpus · 36 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2017 Genetic Complexity of Autosomal Dominant Polycystic Kidney and Liver Diseases. Journal of the American Society of Nephrology : JASN 281 29038287
2018 Prevalence Estimates of Polycystic Kidney and Liver Disease by Population Sequencing. Journal of the American Society of Nephrology : JASN 221 30135240
2017 Improved split fluorescent proteins for endogenous protein labeling. Nature communications 208 28851864
2017 Isolated polycystic liver disease genes define effectors of polycystin-1 function. The Journal of clinical investigation 130 28375157
2020 Insights into Autosomal Dominant Polycystic Kidney Disease from Genetic Studies. Clinical journal of the American Society of Nephrology : CJASN 106 32690722
2021 Dynamic tracking and identification of tissue-specific secretory proteins in the circulation of live mice. Nature communications 95 34471136
2014 Proteomic analysis of solid pseudopapillary tumor of the pancreas reveals dysfunction of the endoplasmic reticulum protein processing pathway. Molecular & cellular proteomics : MCP 87 24997997
2019 WDR45 contributes to neurodegeneration through regulation of ER homeostasis and neuronal death. Autophagy 82 31204559
2022 Exome Sequencing of a Clinical Population for Autosomal Dominant Polycystic Kidney Disease. JAMA 78 36573973
2022 Human T-bet governs the generation of a distinct subset of CD11chighCD21low B cells. Science immunology 69 35867801
2015 Genetic ablation of ataxin-2 increases several global translation factors in their transcript abundance but decreases translation rate. Neurogenetics 59 25721894
2018 A combination of curcumin and oligomeric proanthocyanidins offer superior anti-tumorigenic properties in colorectal cancer. Scientific reports 55 30218018
2022 Upregulation of ribosome complexes at the blood-brain barrier in Alzheimer's disease patients. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 54 35766008
2019 The Extracellular Matrix Receptor Discoidin Domain Receptor 1 Regulates Collagen Transcription by Translocating to the Nucleus. Journal of the American Society of Nephrology : JASN 42 31383731
2017 Isolated polycystic liver disease genes define effectors of polycystin-1 function. The Journal of clinical investigation 38 28862642
2024 USP20 deubiquitinates and stabilizes the reticulophagy receptor RETREG1/FAM134B to drive reticulophagy. Autophagy 30 38705724
2017 3,4-dihydroxybenzalacetone and caffeic acid phenethyl ester induce preconditioning ER stress and autophagy in SH-SY5Y cells. Journal of cellular physiology 28 28681934
2011 Identification of candidate genes linking systemic inflammation to atherosclerosis; results of a human in vivo LPS infusion study. BMC medical genomics 25 21827714
2019 Genetics of polycystic liver diseases. Current opinion in gastroenterology 24 30652979
2015 mRNA encoding Sec61β, a tail-anchored protein, is localized on the endoplasmic reticulum. Journal of cell science 18 26272916
2020 Let-7b-5p is involved in the response of endoplasmic reticulum stress in acute pulmonary embolism through upregulating the expression of stress-associated endoplasmic reticulum protein 1. IUBMB life 14 32534478
2017 Liver cyst gene knockout in cholangiocytes inhibits cilium formation and Wnt signaling. Human molecular genetics 11 28973524
2024 Clinical manifestation, epidemiology, genetic basis, potential molecular targets, and current treatment of polycystic liver disease. Orphanet journal of rare diseases 10 38671465
2025 SEC61B regulates calcium flux and platelet hyperreactivity in diabetes. The Journal of clinical investigation 8 40829182
2021 Autophagy-mediated reduction of miR-345 contributes to hepatic cystogenesis in polycystic liver disease. JHEP reports : innovation in hepatology 8 34568801
2020 cDNA expression library screening revealed novel functional genes involved in clear cell carcinogenesis of the ovary in vitro. Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology 4 32157937
2025 Chronic diseases alter the platelet rheostat to promote hyperreactivity and thrombosis. The Journal of clinical investigation 3 40829178
2024 Identification of novel endoplasmic reticulum-related genes and their association with immune cell infiltration in major depressive disorder. Journal of affective disorders 3 38604455
2025 The Mechanism of Xuanyu Tongjing Decoction Regulating NOD/NFκB Pathway to Inhibit Ectopic Tissue Inflammation to Reduce Ovarian Damage in Rats with Ovarian Endometriosis. Drug design, development and therapy 2 40231194
2025 Autosomal Dominant Polycystic Kidney Disease-Related Multifocal Renal Cell Carcinoma: A Narrative Iconographic Review. International journal of molecular sciences 2 40362206
2024 Identification of differentially expressed ER stress-related genes and their association with pulmonary arterial hypertension. Respiratory research 2 38789967
2022 Comparative proteomics analysis of human stem cells from dental gingival and periodontal ligament. Proteomics 2 35297194
2023 [Influence of family with sequence similarity 134, member B-mediated reticulophagy on lipopolysaccharide-induced apoptosis of mouse dendritic cells]. Zhonghua shao shang yu chuang mian xiu fu za zhi 1 37805802
2026 Micranthin B alleviates metabolic dysfunction-associated steatohepatitis by targeting G3BP1 to improve stress granule-mediated endoplasmic reticulum stress. Chinese journal of natural medicines 0 42215158
2025 ER stress-driven unfolded protein response fuels aging-related tumor aggressiveness in gliomas. Frontiers in molecular biosciences 0 40718795
2025 TIPE2-Regulated ER-Phagy in Dendritic Cell Function Following Hemorrhagic Shock. Shock (Augusta, Ga.) 0 41697115

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