Affinage

RPLP0

Large ribosomal subunit protein uL10 · UniProt P05388

Round 2 corrected
Length
317 aa
Mass
34.3 kDa
Annotated
2026-04-28
130 papers in source corpus 20 papers cited in narrative 20 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RPLP0 (uL10) is the central scaffold of the eukaryotic 60S ribosomal stalk, forming a pentameric P0·(P1–P2)₂ complex that binds the GTPase-associated domain of 28S rRNA and recruits elongation factors eEF1A and eEF2 to drive translocation during protein synthesis (PMID:3323886, PMID:12060678, PMID:9988728). The protein harbors two distinct P1–P2 dimer-binding sites (residues 199–230 and 231–258 in yeast), and its conserved C-terminal domain is phosphorylated by casein kinase II and is functionally essential when other P-family proteins are absent (PMID:16573688, PMID:1856214, PMID:7657639). Cytoplasmic loading of RPLP0 onto pre-60S subunits requires the phosphatase Yvh1, which displaces the nuclear paralog Mrt4, establishing a regulated late step in ribosome maturation (PMID:19797078). Beyond canonical translation, RPLP0 directly crosslinks to mRNA in vivo and interacts with ricin A chain at the ribosomal active site, and its depletion causes G1 cell-cycle arrest linked to reduced CDK2 expression (PMID:22658674, PMID:7759553, PMID:25433997).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1987 High

    Establishing that mammalian P0, P1, and P2 are the eukaryotic counterparts of the bacterial L10·(L7/L12) stalk complex answered the fundamental question of how the elongation-factor-recruiting stalk is organized in eukaryotes.

    Evidence cDNA cloning, in vitro transcription/translation, and immunoprecipitation showing selective assembly of P0/P1/P2 into 60S subunits

    PMID:3323886

    Open questions at the time
    • Stoichiometry of the eukaryotic stalk complex was not yet resolved
    • No structural data on the arrangement of P0 relative to rRNA
  2. 1991 High

    Demonstration that casein kinase II phosphorylates P0 at its conserved C-terminus both in vitro and in vivo identified the kinase responsible for a long-observed post-translational modification of all stalk proteins.

    Evidence In vitro kinase assay with purified CK II plus metabolic ³²P labeling and tryptic phosphopeptide mapping

    PMID:1856214

    Open questions at the time
    • Functional consequence of P0 phosphorylation on translation was not addressed
    • Phosphatase(s) counteracting CK II phosphorylation were unknown
  3. 1995 High

    Systematic deletion mutagenesis of the P0 C-terminal domain and identification of ricin A chain as a direct P0-binding toxin together defined functionally critical regions of P0 on the ribosome — one required for rRNA interaction, another for P1/P2 binding, and the extreme C-terminus essential in the absence of other P proteins.

    Evidence Sequential deletions with yeast complementation assays; chemical cross-linking of ¹²⁵I-ricin A chain to mammalian ribosomes with tryptic peptide sequencing

    PMID:7657639 PMID:7759553 PMID:8722011

    Open questions at the time
    • Precise residues contacting rRNA were not identified
    • Whether ricin A chain binding to P0 is required for ribosome inactivation was untested
  4. 1998 High

    Phosphomimetic and phospho-null mutations at yeast P0 Ser302 showed that CK II-site phosphorylation is dispensable under normal growth but modulates stress-responsive translation, linking stalk phosphorylation to translational regulation under environmental challenge.

    Evidence Site-directed mutagenesis (S302D/C/T) with growth assays under osmotic and heat stress

    PMID:9843429

    Open questions at the time
    • Specific stress-responsive mRNAs whose translation depends on P0 phosphorylation were not identified
    • Mechanism by which phosphorylation state alters translational selectivity was unknown
  5. 1999 High

    Genetic selection of sordarin-resistant P0 mutants provided direct evidence that P0 physically contacts eEF2 during translocation, functionally coupling the stalk to the GTPase cycle of elongation.

    Evidence Sordarin-resistance alleles mapped to a conserved 10-residue region of yeast L10e/P0; reduced eEF2–ribosome complex stabilization confirmed biochemically

    PMID:9988728

    Open questions at the time
    • Atomic-level contacts between P0 and eEF2 were not resolved
    • Whether P0 mutations alter eEF1A recruitment as well was untested
  6. 2002 High

    In vitro reconstitution of the pentameric P0·(P1–P2)₂ complex demonstrated that heterodimer formation of P1–P2 is a prerequisite for P0-mediated rRNA binding and activation of eEF-2-dependent GTPase activity, defining the minimal functional stalk unit.

    Evidence Gel shift, chemical crosslinking, sedimentation analysis, and polyphenylalanine synthesis assay using silkworm components

    PMID:12060678

    Open questions at the time
    • Whether the same stoichiometry holds in all eukaryotes was assumed but not proven
    • Kinetics of stalk assembly on intact ribosomes were not measured
  7. 2005 High

    Fine-mapping of the P1/P2 binding region on P0 to residues 213–250 and the discovery that this site is partially occluded in free P0 revealed a conformational gating mechanism for stalk assembly and showed that truncated P0 fragments retaining this site suffice to recruit eEF2.

    Evidence Yeast two-hybrid with truncated P0 fragments, Ni-NTA pull-down, eEF2 binding assay

    PMID:15807547

    Open questions at the time
    • Nature of the conformational change exposing the binding site was not structurally resolved
  8. 2006 High

    Identification of two separate P1–P2 dimer-binding sites on P0 (residues 199–230 and 231–258) clarified how the pentameric stalk achieves its two-dimer stoichiometry and showed that the first site plus P1A–P2B dimer is the essential unit for stalk formation.

    Evidence Truncated P0 constructs assayed by pull-down, two-hybrid, and ribosome fractionation in yeast

    PMID:16573688

    Open questions at the time
    • Whether the two binding sites are occupied simultaneously or sequentially during biogenesis was unknown
  9. 2009 High

    Discovery that the phosphatase Yvh1 is required to displace the nuclear P0 paralog Mrt4 from cytoplasmic pre-60S particles, enabling P0 loading, established a regulated late maturation step linking stalk assembly to ribosome biogenesis quality control.

    Evidence YVH1 deletion and MRT4 suppressor mutation in yeast; sucrose gradient fractionation and co-IP of pre-60S intermediates

    PMID:19797078

    Open questions at the time
    • Whether Yvh1 phosphatase activity is catalytically required or whether it acts structurally was not resolved
    • Mammalian ortholog of this pathway was not validated
  10. 2012 Medium

    UV-crosslinking interactome capture identified RPLP0 as a direct mRNA-binding protein in living human cells, expanding its functional repertoire beyond structural ribosomal roles.

    Evidence UV crosslinking and oligo(dT) purification with quantitative mass spectrometry in HeLa cells

    PMID:22658674

    Open questions at the time
    • Specific mRNA targets of RPLP0 were not identified
    • Whether mRNA binding is independent of ribosomal context is unknown
  11. 2013 High

    High-resolution cryo-EM structures of the human 80S ribosome placed RPLP0 within the stalk architecture and revealed metazoan-specific structural layers, providing the first near-atomic view of human uL10 in situ.

    Evidence Cryo-electron microscopy of human and Drosophila 80S ribosomes

    PMID:23636399

    Open questions at the time
    • Flexible C-terminal domain and P1/P2 dimers were poorly resolved
    • Conformational dynamics during elongation factor engagement were not captured
  12. 2014 Medium

    Identification of CTSX as a direct RPLP0 interactor and demonstration that RPLP0 knockdown causes G1 arrest with CDK2 suppression revealed an extra-ribosomal role in cell-cycle progression.

    Evidence Yeast two-hybrid, co-IP, siRNA knockdown, and cell cycle analysis in gastric cancer cells

    PMID:25433997

    Open questions at the time
    • Whether the cell-cycle effect is translation-dependent or reflects a moonlighting function is unresolved
    • Mechanism by which CTSX–RPLP0 interaction regulates CDK2 was not determined
  13. 2019 Medium

    PLAAT4 was shown to suppress RPLP0 protein levels, and RPLP0 silencing phenocopied PLAAT4-mediated growth suppression and apoptosis, placing RPLP0 as a downstream effector in a tumor-suppressive pathway.

    Evidence Yeast two-hybrid, co-IP, colocalization, siRNA knockdown with viability and apoptosis assays

    PMID:31131438

    Open questions at the time
    • Mechanism of PLAAT4-mediated RPLP0 degradation or suppression was not elucidated
    • Generalizability beyond the cell lines tested is unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include whether RPLP0's mRNA-binding and cell-cycle regulatory activities are independent of its ribosomal function, the structural basis of its flexible C-terminal domain during elongation factor engagement, and the conservation of the Yvh1/Mrt4 stalk-loading pathway in mammals.
  • No separation-of-function mutant distinguishing ribosomal from extra-ribosomal roles exists
  • High-resolution structure of the fully assembled human stalk with P1/P2 C-termini is lacking
  • Mammalian Yvh1-dependent P0 loading has not been validated

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0045182 translation regulator activity 2 GO:0003723 RNA binding 1
Localization
GO:0005840 ribosome 5 GO:0005730 nucleolus 1 GO:0005829 cytosol 1
Pathway
R-HSA-392499 Metabolism of proteins 4 R-HSA-1640170 Cell Cycle 2
Partners
CTSXEEF2MRT4PLAAT4RPLP1RPLP2YVH1
Complex memberships
60S ribosomal subunitP0·(P1–P2)₂ ribosomal stalk complex

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1987 Human ribosomal phosphoproteins P0, P1, and P2 were cloned and shown to encode proteins analogous to bacterial L10 (P0) and L7/L12 (P1/P2). In vitro synthesized P0, P1, and P2 proteins assembled specifically into 60S but not 40S ribosomal subunits, and formed a complex analogous to the bacterial L7/L12·L10 complex. cDNA cloning, in vitro transcription/translation, ribosome assembly assay, immunoprecipitation Molecular and cellular biology High 3323886
1991 Ribosomal proteins P0, P1, and P2 are phosphorylated at their conserved carboxyl termini by casein kinase II (CK II). Both exogenous purified CK II and an endogenous ribosome-associated CK II-like enzyme phosphorylated all three P proteins selectively in vitro, and tryptic phosphopeptide mapping confirmed that the same COOH-terminal sites are phosphorylated in intact cells metabolically labeled with [32P]orthophosphate. In vitro kinase assay with purified CK II, metabolic 32P labeling, tryptic phosphopeptide mapping The Journal of biological chemistry High 1856214
1995 The carboxyl-terminal domain of yeast P0 (approximately 100 amino acids) is essential for ribosome activity only when P1 and P2 proteins are absent. Sequential deletions showed that the region around residues 185–230 is required for P0 interaction with rRNA, while the region 230–290 is important for P1/P2 interaction with the ribosome. Removal of the last 21 amino acids is lethal in strains lacking P1/P2, demonstrating that at least one P-like C-terminal peptide must be ribosome-bound for protein synthesis. Sequential deletion mutagenesis of P0 C-terminal domain, yeast complementation assay in P0 conditional null and quadruple P-protein disruptant strains The Journal of biological chemistry High 7657639
1995 The eukaryotic ribosomal stalk consists of phosphoproteins P1 and P2 in complex with P0 at the GTPase domain of large subunit rRNA. P1/P2 form an unusual cytoplasmic pool of dephosphorylated proteins that dynamically exchange with ribosome-bound forms during translation. Quadruply disrupted yeast strains lacking all acidic proteins are viable but show reduced growth and altered in vivo/in vitro translation of specific mRNAs, indicating that P1/P2 modulate ribosomal activity for specific transcripts rather than being essential for overall protein synthesis. Yeast genetics (quadruple acidic protein gene disruption), in vitro translation, 2D gel electrophoresis of expressed proteome Biochemistry and cell biology High 8722011
1998 Phosphorylation of yeast P0 at serine 302 (within a CK II consensus site SDDD) is not required for overall ribosome activity under standard conditions, but overphosphorylation (serine-to-threonine substitution) impairs cell growth under osmotic and heat stress, and in vitro polymerization data suggest this is not due to protein thermosensitivity but rather affects expression of stress-responsive proteins. Site-directed mutagenesis (S302D, S302C, S302T), in vivo and in vitro phosphorylation assays, yeast growth complementation under stress conditions Biochemistry High 9843429
1999 Mutations in the essential large ribosomal subunit protein L10e (the yeast RPLP0 ortholog) confer resistance to the eEF2 inhibitor sordarin. Five unique L10e alleles with amino acid substitutions or deletions in a conserved 10-amino acid region showed reduced sordarin-conferred stabilization of the eEF2-nucleotide-ribosome complex, revealing a direct functional linkage between L10e/P0 and eEF2 during translocation. Genetic selection for sordarin resistance, biochemical analysis of eEF2-ribosome complex stability, sequencing of resistant alleles The Journal of biological chemistry High 9988728
2002 Silkworm ribosomal proteins P1 and P2 form a P1-P2 heterodimer, which binds to P0 to form a pentameric P0(P1-P2)2 complex. This complex formation strongly stimulates P0 binding to the GTPase-associated domain of 28S rRNA (residues 1030–1127). Both P1 and P2, together with P0 and eL12, are required to activate ribosomes in polyphenylalanine synthesis dependent on eukaryotic elongation factors and eEF-2-dependent GTPase activity. Gel mobility shift, chemical crosslinking, sedimentation analysis, in vitro reconstitution of ribosome activity, polyphenylalanine synthesis assay, eEF-2-dependent GTPase assay Nucleic acids research High 12060678
2002 Overexpression of the acidic ribosomal protein Rpp0 (yeast RPLP0 ortholog) can cure yeast prion determinants [PSI+PS] and affects manifestation of conventional [PSI+] in a strain-specific manner. Excess Rpp0 influenced chaperone-specific promoters (SSA4, HSP104), suggesting its prion-curing effect may operate through induction of chaperone expression. Multicopy suppressor screen from yeast genomic library, prion curing assay, promoter reporter assays The Journal of biological chemistry Medium 11923285
2005 The acidic protein (P1/P2) binding site on yeast P0 is partially hidden in the free protein, requiring conformational changes for stalk assembly. Using truncated P0 fragments in two-hybrid and Ni-NTA pull-down assays, a region between positions 213–250 was identified as the minimal fragment interacting with P1/P2. Extensions at either end of this fragment modulate binding capacity, and deletion within this region severely reduces P0-P1/P2 interaction and acidic protein incorporation into ribosomes. P0 fragments containing the binding site and associated P1/P2 can recruit elongation factor EF2. Yeast two-hybrid with truncated P0 fragments, Ni-NTA pull-down, EF2 binding assay Biochemistry High 15807547
2006 Yeast P0 has two separate and independent binding sites for P1/P2 protein dimers, located between positions 199–230 and 231–258 respectively. These two short regions independently bind the P1A-P2B and P1B-P2A dimers. The sequence spanning 199–230 and the P1A-P2B dimer are essential for stalk formation, indicating a balance between this dimer and P0 governs stalk assembly. Truncated P0 constructs tested by in vitro and in vivo binding assays (pull-down, two-hybrid, ribosome fractionation) Molecular microbiology High 16573688
2009 Assembly of the mature ribosomal stalk requires the dual-specificity phosphatase Yvh1 to mediate displacement of the nuclear P0 paralog Mrt4 from pre-60S subunits in the cytoplasm, allowing P0 to load onto the subunit. Pre-60S subunits associated with Yvh1 contain Rpl12 but lack both Mrt4 and P0. A mutation in Mrt4 at the protein-RNA interface bypasses the requirement for Yvh1, placing Yvh1 activity upstream of P0 loading. Yeast genetics (YVH1 deletion, MRT4 suppressor mutation), sucrose gradient fractionation, co-immunoprecipitation, ribosome profiling The Journal of cell biology High 19797078
2013 Cryo-EM structures of human and Drosophila 80S ribosomes reveal the positions of ribosomal proteins including RPLP0 and the stalk architecture in the context of the complete eukaryotic ribosome, showing co-evolution of metazoan-specific rRNA expansion segments with ribosomal proteins and two additional structural layers unique to metazoan ribosomes. High-resolution cryo-electron microscopy structural determination Nature High 23636399
2014 RPLP0 interacts with cathepsin X/Z (CTSX) in gastric cancer cells, as identified by yeast two-hybrid analysis and confirmed by co-immunoprecipitation. Laser scan microscopy revealed shuttling of RPLP0 from cytoplasm to nucleus following CTSX knockdown. Knockdown of RPLP0 caused G1 cell cycle arrest and suppressed CDK2 expression, while affecting p21 but not Cyclin E levels, indicating RPLP0 participates in cell cycle progression regulation in gastric cancer. Yeast two-hybrid, co-immunoprecipitation, siRNA knockdown, laser scan confocal microscopy, cell cycle analysis, western blot Pathology, research and practice Medium 25433997
2019 RPLP0 interacts with the tumor suppressor PLAAT4 (phospholipase A and acyltransferase 4), as demonstrated by yeast two-hybrid screening, co-immunoprecipitation, and colocalization. RPLP0 protein levels are suppressed in PLAAT4-expressing cells. RPLP0 silencing phenocopies PLAAT4 expression: both conditions decrease cell viability and proliferation, increase cell death, and reduce levels of cell cycle-associated and anti-apoptotic proteins, placing RPLP0 downstream of PLAAT4 in a growth-suppressive pathway. Yeast two-hybrid, co-immunoprecipitation, colocalization (confocal microscopy), siRNA knockdown, cell viability/proliferation assays, western blot for cell cycle proteins Cell biochemistry and biophysics Medium 31131438
1995 Chemical cross-linking experiments identified RPLP0 (L10e) as one of two mammalian large ribosomal subunit proteins (L9 and L10e) that directly bind ricin A chain. The specificity was demonstrated by competition with excess unlabeled ricin A chain but not gelonin, and the interacting proteins were identified by amino acid sequencing of tryptic peptides from purified cross-linked complexes. Chemical cross-linking with 125I-labeled ricin A chain, purification of cross-linked complexes, amino acid sequencing of tryptic peptides The Journal of biological chemistry High 7759553
2002 RPLP0 was identified as a component of the human nucleolus by directed proteomic analysis, establishing its nucleolar localization in human cells. Mass spectrometry-based proteomic analysis of isolated human nucleoli Current biology : CB Medium 11790298
2006 RPLP0 was identified as a phosphoprotein in HeLa cells with multiple phosphorylation sites detected by mass spectrometry in a global phosphoproteomics study; phosphorylation sites were dynamically regulated upon EGF stimulation. Mass spectrometry-based phosphoproteomics (SILAC quantitation) of EGF-stimulated HeLa cells Cell Medium 17081983
2012 RPLP0 was identified as an mRNA-binding protein in human HeLa cells through interactome capture using UV crosslinking and oligo(dT) purification, demonstrating direct physical association with mRNA in vivo. UV crosslinking, oligo(dT) affinity purification, quantitative mass spectrometry (interactome capture) Cell Medium 22658674
2015 RPLP0 was identified as a component of the human protein interactome through high-throughput affinity-purification mass spectrometry (BioPlex), placing it within a defined protein community corresponding to the ribosomal large subunit complex. Affinity-purification mass spectrometry (AP-MS) in HEK293T cells Cell Medium 26186194
2022 RPLP0 localizes to the cytoplasm (consistent with ribosomal large subunit localization) as determined by endogenous GFP tagging and confocal live-cell imaging in human cells, with interaction partners identified by immunoprecipitation mass spectrometry confirming association with the 60S ribosomal subunit complex. Endogenous GFP tagging, confocal live-cell imaging, immunoprecipitation mass spectrometry Science Medium 35271311

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 2861 17081983
2012 Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell 1718 22658674
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2011 Systematic and quantitative assessment of the ubiquitin-modified proteome. Molecular cell 1334 21906983
2004 Large-scale characterization of HeLa cell nuclear phosphoproteins. Proceedings of the National Academy of Sciences of the United States of America 1159 15302935
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2012 The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. Molecular cell 973 22681889
2004 Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nature biotechnology 916 15592455
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2002 Directed proteomic analysis of the human nucleolus. Current biology : CB 780 11790298
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2011 A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. Molecular & cellular proteomics : MCP 749 21890473
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2018 High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies. Molecular cell 580 29395067
2017 Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Science (New York, N.Y.) 533 28302793
2016 G3BP-Caprin1-USP10 complexes mediate stress granule condensation and associate with 40S subunits. The Journal of cell biology 512 27022092
1994 Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene 492 8125298
2011 Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation. Nature cell biology 490 21423176
2013 Structures of the human and Drosophila 80S ribosome. Nature 481 23636399
2020 SARS-CoV-2 Disrupts Splicing, Translation, and Protein Trafficking to Suppress Host Defenses. Cell 449 33080218
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2010 Global analysis of TDP-43 interacting proteins reveals strong association with RNA splicing and translation machinery. Journal of proteome research 422 20020773
2013 The intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes. The Journal of biological chemistry 413 23463506
1991 The effects of cAMP on differentiation of cultured Schwann cells: progression from an early phenotype (04+) to a myelin phenotype (P0+, GFAP-, N-CAM-, NGF-receptor-) depends on growth inhibition. The Journal of cell biology 337 1704008
1996 Crystal structure of the extracellular domain from P0, the major structural protein of peripheral nerve myelin. Neuron 335 8816707
1990 Role of myelin P0 protein as a homophilic adhesion molecule. Nature 320 1691824
1996 Clinical phenotypes of different MPZ (P0) mutations may include Charcot-Marie-Tooth type 1B, Dejerine-Sottas, and congenital hypomyelination. Neuron 317 8816708
2007 The Polerovirus silencing suppressor P0 targets ARGONAUTE proteins for degradation. Current biology : CB 282 17869110
1987 Human acidic ribosomal phosphoproteins P0, P1, and P2: analysis of cDNA clones, in vitro synthesis, and assembly. Molecular and cellular biology 275 3323886
2007 The Polerovirus F box protein P0 targets ARGONAUTE1 to suppress RNA silencing. Current biology : CB 243 17869109
2006 F-box-like domain in the polerovirus protein P0 is required for silencing suppressor function. Proceedings of the National Academy of Sciences of the United States of America 202 16446454
1993 Deletion of the serine 34 codon from the major peripheral myelin protein P0 gene in Charcot-Marie-Tooth disease type 1B. Nature genetics 190 7693130
1981 Immunocytochemical localization of P0 protein in Golgi complex membranes and myelin of developing rat Schwann cells. The Journal of cell biology 176 6166623
2002 P0 of beet Western yellows virus is a suppressor of posttranscriptional gene silencing. Journal of virology 153 12050394
1996 Structure of the HNK-1 carbohydrate epitope on bovine peripheral myelin glycoprotein P0. The Journal of biological chemistry 148 8798480
1999 P0 and PMP22 mark a multipotent neural crest-derived cell type that displays community effects in response to TGF-beta family factors. Development (Cambridge, England) 144 10433908
1990 Recombinant peripheral myelin protein P0 confers both adhesion and neurite outgrowth-promoting properties. Journal of neuroscience research 123 1711125
1995 Molecular mimicry between the immunodominant ribosomal protein P0 of Trypanosoma cruzi and a functional epitope on the human beta 1-adrenergic receptor. The Journal of experimental medicine 119 7790824
2006 Imprinting of IGF2 P0 transcript and novel alternatively spliced INS-IGF2 isoforms show differences between mouse and human. Human molecular genetics 112 16531418
1992 P0 promoter directs expression of reporter and toxin genes to Schwann cells of transgenic mice. Neuron 102 1372510
1994 Rapid screening of myelin genes in CMT1 patients by SSCP analysis: identification of new mutations and polymorphisms in the P0 gene. Human genetics 98 7527371
1995 Proteins P1, P2, and P0, components of the eukaryotic ribosome stalk. New structural and functional aspects. Biochemistry and cell biology = Biochimie et biologie cellulaire 97 8722011
1993 Production and characterization of monoclonal antibodies to the extracellular domain of P0. Journal of neuroscience research 97 7685397
2009 Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0. The Journal of cell biology 96 19797078
1993 Mutation of the myelin P0 gene in Charcot-Marie-Tooth neuropathy type 1B. Human molecular genetics 96 7694726
1996 Functional abnormalities in P0-deficient mice resemble human hereditary neuropathies linked to P0 gene mutations. Muscle & nerve 87 8756159
1995 The highly conserved protein P0 carboxyl end is essential for ribosome activity only in the absence of proteins P1 and P2. The Journal of biological chemistry 87 7657639
2019 The viral F-box protein P0 induces an ER-derived autophagy degradation pathway for the clearance of membrane-bound AGO1. Proceedings of the National Academy of Sciences of the United States of America 84 31628252
1993 Homophilic adhesion of the myelin P0 protein requires glycosylation of both molecules in the homophilic pair. The Journal of cell biology 81 7686552
1991 Ribosomal proteins P0, P1, and P2 are phosphorylated by casein kinase II at their conserved carboxyl termini. The Journal of biological chemistry 80 1856214
1993 Mutation of the myelin P0 gene in Charcot-Marie-tooth neuropathy type 1. Biochemical and biophysical research communications 79 7688964
1999 Loss of distal axons and sensory Merkel cells and features indicative of muscle denervation in hindlimbs of P0-deficient mice. The Journal of neuroscience : the official journal of the Society for Neuroscience 77 10407042
1988 Distribution of the myelin-associated glycoprotein and P0 protein during myelin compaction in quaking mouse peripheral nerve. The Journal of cell biology 77 2458358
1993 Antibody responses to P0 and P2 myelin proteins in Guillain-Barré syndrome and chronic idiopathic demyelinating polyradiculoneuropathy. Journal of neuroimmunology 75 7689591
2010 Expression of the ribosomal proteins Rplp0, Rplp1, and Rplp2 in gynecologic tumors. Human pathology 74 21040949
2006 Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteins. Molecular microbiology 73 16573688
1991 The role of complex carbohydrates in adhesion of the myelin protein, P0. Neuron 72 1720626
2002 Increased expression of Hsp40 chaperones, transcriptional factors, and ribosomal protein Rpp0 can cure yeast prions. The Journal of biological chemistry 68 11923285
2002 Interaction among silkworm ribosomal proteins P1, P2 and P0 required for functional protein binding to the GTPase-associated domain of 28S rRNA. Nucleic acids research 68 12060678
2001 Mutations in the cytoplasmic domain of P0 reveal a role for PKC-mediated phosphorylation in adhesion and myelination. The Journal of cell biology 68 11673479
1994 The cytoplasmic domain of the myelin P0 protein influences the adhesive interactions of its extracellular domain. The Journal of cell biology 67 7519618
2008 The P0 gene of Sugarcane yellow leaf virus encodes an RNA silencing suppressor with unique activities. Virology 66 19046592
2000 Charcot-Marie-Tooth neuropathy type 2 and P0 point mutations: two novel amino acid substitutions (Asp61Gly; Tyr119Cys) and a possible "hotspot" on Thr124Met. Brain pathology (Zurich, Switzerland) 59 10764043
1999 A novel P0 glycoprotein transgene activates expression of lacZ in myelin-forming Schwann cells. The European journal of neuroscience 54 10215910
2008 Primary Culture of Hippocampal Neurons from P0 Newborn Rats. Journal of visualized experiments : JoVE 53 19066540
2002 Myelin P0: new knowledge and new roles. Neurochemical research 52 12512938
2000 Schwann cell myelination requires timely and precise targeting of P(0) protein. The Journal of cell biology 52 10704450
2017 Specific and spatial labeling of P0-Cre versus Wnt1-Cre in cranial neural crest in early mouse embryos. Genesis (New York, N.Y. : 2000) 49 28371069
1993 New mutation of the myelin P0 gene in a pedigree of Charcot-Marie-Tooth neuropathy 1. Biochemistry and molecular biology international 49 7505151
2012 L-MPZ, a novel isoform of myelin P0, is produced by stop codon readthrough. The Journal of biological chemistry 48 22457349
2004 Altered expression of ion channel isoforms at the node of Ranvier in P0-deficient myelin mutants. Molecular and cellular neurosciences 48 14962742
1999 Mutations in ribosomal protein L10e confer resistance to the fungal-specific eukaryotic elongation factor 2 inhibitor sordarin. The Journal of biological chemistry 47 9988728
1995 Myelin P0 glycoprotein and a synthetic peptide containing the palmitoylation site are both autoacylated. Journal of neurochemistry 46 7561879
2009 P0 proteins of European beet-infecting poleroviruses display variable RNA silencing suppression activity. The Journal of general virology 45 19955562
1994 Antigens shared by Leishmania species and Trypanosoma cruzi: immunological comparison of the acidic ribosomal P0 proteins. Infection and immunity 45 7513304
1998 Phosphorylation of ribosomal protein P0 is not essential for ribosome function but can affect translation. Biochemistry 44 9843429
1992 Molecular mimicry and the autoimmune response to the peripheral nerve myelin P0 glycoprotein. Neurochemical research 44 1383842
1991 Expressing antisense P0 RNA in Schwann cells perturbs myelination. Development (Cambridge, England) 43 1724420
1995 Ricin A chain can be chemically cross-linked to the mammalian ribosomal proteins L9 and L10e. The Journal of biological chemistry 42 7759553
2014 Dysregulation of apoptotic signaling pathways by interaction of RPLP0 and cathepsin X/Z in gastric cancer. Pathology, research and practice 41 25433997
2001 Investigation of serum response to PMP22, connexin 32 and P(0) in inflammatory neuropathies. Journal of neuroimmunology 41 11438177
2009 A novel myelin P0-specific T cell receptor transgenic mouse develops a fulminant autoimmune peripheral neuropathy. The Journal of experimental medicine 40 19221395
2019 Molecular structure and function of myelin protein P0 in membrane stacking. Scientific reports 39 30679613
2018 A Suppressor Screen for AGO1 Degradation by the Viral F-Box P0 Protein Uncovers a Role for AGO DUF1785 in sRNA Duplex Unwinding. The Plant cell 39 29848768
2014 Elicitation of hypersensitive responses in Nicotiana glutinosa by the suppressor of RNA silencing protein P0 from poleroviruses. Molecular plant pathology 35 25187258
2015 Association of anti-acidic ribosomal protein P0 and anti-galectin 3 antibodies with the development of skin lesions in systemic lupus erythematosus. Arthritis & rheumatology (Hoboken, N.J.) 34 25307291
2000 P0 protein peptide 180-199 together with pertussis toxin induces experimental autoimmune neuritis in resistant C57BL/6 mice. Journal of neuroscience research 34 11104510
2015 HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins. PLoS biology 33 26406915
1995 Myelin glycoprotein P0 is expressed at early stages of chicken and rat embryogenesis. Journal of neuroscience research 33 7745617
1993 Myelin P0-glycoprotein: predicted structure and interactions of extracellular domain. Journal of neurochemistry 33 7504078
1987 A phorbol ester-sensitive kinase catalyzes the phosphorylation of P0 glycoprotein in myelin. Journal of neurochemistry 33 2445920
2004 The P domain of the P0 protein of Plasmodium falciparum protects against challenge with malaria parasites. Infection and immunity 32 15322057
1990 Occurrence in the archaebacterium Sulfolobus solfataricus of a ribosomal protein complex corresponding to Escherichia coli (L7/L12)4.L10 and eukaryotic (P1)2/(P2)2.P0. The Journal of biological chemistry 29 2121730
1998 The glycoprotein P0 in peripheral gliogenesis. Cell and tissue research 28 9506907
1995 Identification of the Leishmania infantum P0 ribosomal protein epitope in canine visceral leishmaniasis. Immunology letters 28 8847086
1987 Metabolism of phosphate and sulfate groups modifying the P0 protein of peripheral nervous system myelin. Journal of neurochemistry 28 2433400
2013 The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing. Virus research 27 24370867
2001 P0 glycoprotein peptides 56-71 and 180-199 dose-dependently induce acute and chronic experimental autoimmune neuritis in Lewis rats associated with epitope spreading. Journal of neuroimmunology 26 11240020
1999 Localization and functional roles of PMP22 in peripheral nerves of P0-deficient mice. Glia 26 10559784
1995 Molecular cloning and tissue expression of a cDNA encoding IP1--a P0-like glycoprotein of trout CNS myelin. Journal of neurochemistry 26 7539051
2020 Functional and Mass Spectrometric Evaluation of an Anti-Tick Antigen Based on the P0 Peptide Conjugated to Bm86 Protein. Pathogens (Basel, Switzerland) 25 32630414
2018 Liposome mediated double-stranded RNA delivery to silence ribosomal protein P0 in the tick Rhipicephalus haemaphysaloides. Ticks and tick-borne diseases 25 29444753
1993 Detection and quantification of antibodies to the extracellular domain of P0 during experimental allergic neuritis. Journal of the neurological sciences 25 7691994
2019 The Ribosomal Protein RPLP0 Mediates PLAAT4-induced Cell Cycle Arrest and Cell Apoptosis. Cell biochemistry and biophysics 24 31131438
2015 Function and diversity of P0 proteins among cotton leafroll dwarf virus isolates. Virology journal 24 26260343
2007 Gene silencing of ribosomal protein P0 is lethal to the tick Haemaphysalis longicornis. Veterinary parasitology 24 18155838
1992 Myelin P0-protein, more than just a structural protein? BioEssays : news and reviews in molecular, cellular and developmental biology 24 1285424
2005 The acidic protein binding site is partially hidden in the free Saccharomyces cerevisiae ribosomal stalk protein P0. Biochemistry 23 15807547
2004 Comparative proteomics of the Mycobacterium leprae binding protein myelin P0: its implication in leprosy and other neurodegenerative diseases. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 23 15019586
2000 Sequence and transcription patterns of 60S ribosomal protein P0, a diapause-regulated AP endonuclease in the flesh fly, Sarcophaga crassipalpis. Gene 23 11024299
1991 Distribution of P0 protein and the myelin-associated glycoprotein in peripheral nerves from Trembler mice. Journal of neurocytology 23 1714491
2011 Major isoform of zebrafish P0 is a 23.5 kDa myelin glycoprotein expressed in selected white matter tracts of the central nervous system. The Journal of comparative neurology 22 21452240
2011 Specialization of endoplasmic reticulum chaperones for the folding and function of myelin glycoproteins P0 and PMP22. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22 21831887
2003 Heterozygous null mutation of myelin P0 protein enhances susceptibility to autoimmune neuritis targeting P0 peptide. European journal of immunology 22 12616486
2003 Translocation of ribosomal protein P0 onto the Toxoplasma gondii tachyzoite surface. International journal for parasitology 22 14636674
1990 High level of expression of the myelin protein P0 in Chinese hamster ovary cells. Journal of neurochemistry 22 1695238
1999 Further examination of the Xist promoter-switch hypothesis in X inactivation: evidence against the existence and function of a P(0) promoter. Proceedings of the National Academy of Sciences of the United States of America 21 10588721
1995 Myelin P0 glycoprotein: identification of the site phosphorylated in vitro and in vivo by endogenous protein kinases. Journal of neurochemistry 21 7530295
1992 Characterization of the C-terminal region of a Trypanosoma cruzi 38-kDa ribosomal P0 protein that does not react with lupus anti-P autoantibodies. Immunology letters 21 1385317
1981 Immunocytochemical study of P0 glycoprotein, P1 and P2 basic proteins, and myelin-associated glycoprotein (MAG) in lesions of idiopathic polyneuritis. Neuropathology and applied neurobiology 21 6173794
2018 Brassica yellows virus P0 protein impairs the antiviral activity of NbRAF2 in Nicotiana benthamiana. Journal of experimental botany 20 29659986
2005 Functional complementation of yeast ribosomal P0 protein with Plasmodium falciparum P0. Gene 20 16099608
1996 Monoclonal antibodies specific to the integral membrane protein P0 of bovine peripheral nerve myelin. Neuroscience research 19 8808799