Affinage

RNF4

E3 ubiquitin-protein ligase RNF4 · UniProt P78317

Audit flag: rerun needed
Length
190 aa
Mass
21.3 kDa
Annotated
2026-04-28
130 papers in source corpus

Mechanistic narrative

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Mechanism profile

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Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2008 RNF4 is a poly-SUMO-specific E3 ubiquitin ligase required for arsenic-induced PML degradation. Nature cell biology 691 18408734
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2008 Arsenic degrades PML or PML-RARalpha through a SUMO-triggered RNF4/ubiquitin-mediated pathway. Nature cell biology 607 18408733
2010 An atlas of combinatorial transcriptional regulation in mouse and man. Cell 573 20211142
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2005 Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome research 409 16344560
1996 Normalization and subtraction: two approaches to facilitate gene discovery. Genome research 401 8889548
2001 The IC-SNURF-SNRPN transcript serves as a host for multiple small nucleolar RNA species and as an antisense RNA for UBE3A. Human molecular genetics 299 11726556
2007 SUMO-targeted ubiquitin ligases in genome stability. The EMBO journal 293 17762865
2017 Genome-wide CRISPR screen identifies HNRNPL as a prostate cancer dependency regulating RNA splicing. Proceedings of the National Academy of Sciences of the United States of America 282 28611215
2012 RNF4, a SUMO-targeted ubiquitin E3 ligase, promotes DNA double-strand break repair. Genes & development 276 22661229
2007 Conserved function of RNF4 family proteins in eukaryotes: targeting a ubiquitin ligase to SUMOylated proteins. The EMBO journal 251 17762864
2004 Functional proteomics mapping of a human signaling pathway. Genome research 247 15231748
2019 Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS chemical biology 245 31059647
2012 SUMO-targeted ubiquitin E3 ligase RNF4 is required for the response of human cells to DNA damage. Genes & development 209 22661230
2014 Oxidative stress-induced assembly of PML nuclear bodies controls sumoylation of partner proteins. The Journal of cell biology 185 24637324
1998 Identification of a novel RING finger protein as a coregulator in steroid receptor-mediated gene transcription. Molecular and cellular biology 177 9710597
2013 The protein interaction landscape of the human CMGC kinase group. Cell reports 174 23602568
2010 A human MAP kinase interactome. Nature methods 165 20936779
2014 A cellular system that degrades misfolded proteins and protects against neurodegeneration. Molecular cell 157 24882209
2012 RNF4-dependent hybrid SUMO-ubiquitin chains are signals for RAP80 and thereby mediate the recruitment of BRCA1 to sites of DNA damage. Science signaling 153 23211528
2011 Mechanism of ubiquitylation by dimeric RING ligase RNF4. Nature structural & molecular biology 149 21857666
2017 RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination. BMC biology 135 29117863
2011 Purification and identification of endogenous polySUMO conjugates. EMBO reports 134 21252943
2004 Regulation of the large (approximately 1000 kb) imprinted murine Ube3a antisense transcript by alternative exons upstream of Snurf/Snrpn. Nucleic acids research 134 15226413
2009 A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system. Molecular systems biology 130 19690564
2009 Analysis of the human E2 ubiquitin conjugating enzyme protein interaction network. Genome research 129 19549727
2007 The Slx5-Slx8 complex affects sumoylation of DNA repair proteins and negatively regulates recombination. Molecular and cellular biology 124 17591698
2022 The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin. Nature cell biology 122 35013556
2016 USP7 is a SUMO deubiquitinase essential for DNA replication. Nature structural & molecular biology 119 26950370
2007 Toward a confocal subcellular atlas of the human proteome. Molecular & cellular proteomics : MCP 114 18029348
2020 A conserved SUMO pathway repairs topoisomerase DNA-protein cross-links by engaging ubiquitin-mediated proteasomal degradation. Science advances 112 33188014
2010 The SUMO protease SENP6 is essential for inner kinetochore assembly. The Journal of cell biology 108 20212317
2010 Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Molecular medicine (Cambridge, Mass.) 108 20379614
2021 Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity. Nature communications 107 34599178
2016 PolySUMOylation by Siz2 and Mms21 triggers relocation of DNA breaks to nuclear pores through the Slx5/Slx8 STUbL. Genes & development 103 27056668
2020 The Nuclear SUMO-Targeted Ubiquitin Quality Control Network Regulates the Dynamics of Cytoplasmic Stress Granules. Molecular cell 101 32521226
2012 RNF4 is required for DNA double-strand break repair in vivo. Cell death and differentiation 100 23197296
2004 Negative modulation of androgen receptor transcriptional activity by Daxx. Molecular and cellular biology 98 15572661
2015 Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains. Nature structural & molecular biology 97 26148049
2000 The RING finger protein SNURF modulates nuclear trafficking of the androgen receptor. Journal of cell science 95 10934038
2013 RNF4 and PLK1 are required for replication fork collapse in ATR-deficient cells. Genes & development 91 24142876
2015 c-Myc is targeted to the proteasome for degradation in a SUMOylation-dependent manner, regulated by PIAS1, SENP7 and RNF4. Cell cycle (Georgetown, Tex.) 87 25895136
2008 Activation of the Slx5-Slx8 ubiquitin ligase by poly-small ubiquitin-like modifier conjugates. The Journal of biological chemistry 86 18499666
2005 Suppression of genomic instability by SLX5 and SLX8 in Saccharomyces cerevisiae. DNA repair 86 16325482
2017 The STUbL RNF4 regulates protein group SUMOylation by targeting the SUMO conjugation machinery. Nature communications 85 29180619
2000 A novel member of the BTB/POZ family, PATZ, associates with the RNF4 RING finger protein and acts as a transcriptional repressor. The Journal of biological chemistry 81 10713105
2009 RNF4 and VHL regulate the proteasomal degradation of SUMO-conjugated Hypoxia-Inducible Factor-2alpha. Nucleic acids research 79 20026589
2014 SUMO chain-induced dimerization activates RNF4. Molecular cell 72 24656128
2010 RING domain dimerization is essential for RNF4 function. The Biochemical journal 72 20681948
2017 Ataxin-3 consolidates the MDC1-dependent DNA double-strand break response by counteracting the SUMO-targeted ubiquitin ligase RNF4. The EMBO journal 71 28275011
2013 Distinct SUMO ligases cooperate with Esc2 and Slx5 to suppress duplication-mediated genome rearrangements. PLoS genetics 71 23935535
2010 Arsenic-induced SUMO-dependent recruitment of RNF4 into PML nuclear bodies. Molecular biology of the cell 68 20943951
2016 SUMO-Targeted Ubiquitin Ligase (STUbL) Slx5 regulates proteolysis of centromeric histone H3 variant Cse4 and prevents its mislocalization to euchromatin. Molecular biology of the cell 67 26960795
2014 ATL response to arsenic/interferon therapy is triggered by SUMO/PML/RNF4-dependent Tax degradation. Blood 65 25395419
2005 Genetic analysis connects SLX5 and SLX8 to the SUMO pathway in Saccharomyces cerevisiae. Genetics 65 16387868
2000 Coregulator small nuclear RING finger protein (SNURF) enhances Sp1- and steroid receptor-mediated transcription by different mechanisms. The Journal of biological chemistry 65 10617653
2004 SUMO-1 promotes association of SNURF (RNF4) with PML nuclear bodies. Experimental cell research 63 15707587
2007 Fission yeast Rnf4 homologs are required for DNA repair. The Journal of biological chemistry 61 17502373
2010 Identification of RING finger protein 4 (RNF4) as a modulator of DNA demethylation through a functional genomics screen. Proceedings of the National Academy of Sciences of the United States of America 58 20696907
2021 Mechanism and function of DNA replication-independent DNA-protein crosslink repair via the SUMO-RNF4 pathway. The EMBO journal 57 34346517
2017 Manipulating PML SUMOylation via Silencing UBC9 and RNF4 Regulates Cardiac Fibrosis. Molecular therapy : the journal of the American Society of Gene Therapy 55 28143738
2009 Role of SUMO in RNF4-mediated promyelocytic leukemia protein (PML) degradation: sumoylation of PML and phospho-switch control of its SUMO binding domain dissected in living cells. The Journal of biological chemistry 51 19380586
2001 A translocation breakpoint cluster disrupts the newly defined 3' end of the SNURF-SNRPN transcription unit on chromosome 15. Human molecular genetics 51 11159938
2016 RNF4-Dependent Oncogene Activation by Protein Stabilization. Cell reports 48 27653698
2011 The Sumo-targeted ubiquitin ligase RNF4 regulates the localization and function of the HTLV-1 oncoprotein Tax. Blood 47 22106342
2010 Wss1 is a SUMO-dependent isopeptidase that interacts genetically with the Slx5-Slx8 SUMO-targeted ubiquitin ligase. Molecular and cellular biology 46 20516210
2016 miR-762 promotes porcine immature Sertoli cell growth via the ring finger protein 4 (RNF4) gene. Scientific reports 45 27596571
2023 Compartmentalization of the SUMO/RNF4 pathway by SLX4 drives DNA repair. Molecular cell 44 37059091
2014 RNF4 interacts with both SUMO and nucleosomes to promote the DNA damage response. EMBO reports 44 24714598
2007 Stimulation of in vitro sumoylation by Slx5-Slx8: evidence for a functional interaction with the SUMO pathway. DNA repair 44 17669696
2004 Transcriptional coregulator SNURF (RNF4) possesses ubiquitin E3 ligase activity. FEBS letters 44 14987998
2004 SNURF-SNRPN and UBE3A transcript levels in patients with Angelman syndrome. Human genetics 44 15014980
2003 The RING finger protein RNF4, a co-regulator of transcription, interacts with the TRPS1 transcription factor. The Journal of biological chemistry 44 12885770
2001 RNF4 is a growth inhibitor expressed in germ cells but not in human testicular tumors. The American journal of pathology 44 11583949
2000 Interaction between the transcription factor SPBP and the positive cofactor RNF4. An interplay between protein binding zinc fingers. The Journal of biological chemistry 44 10849425
2014 An arginine-rich motif of ring finger protein 4 (RNF4) oversees the recruitment and degradation of the phosphorylated and SUMOylated Krüppel-associated box domain-associated protein 1 (KAP1)/TRIM28 protein during genotoxic stress. The Journal of biological chemistry 41 24907272
2014 PML, SUMO, and RNF4: guardians of nuclear protein quality. Molecular cell 40 24996060
2012 Requirement of PML SUMO interacting motif for RNF4- or arsenic trioxide-induced degradation of nuclear PML isoforms. PloS one 39 23028697
2004 Influence of in vitro manipulation on the stability of methylation patterns in the Snurf/Snrpn-imprinting region in mouse embryonic stem cells. Nucleic acids research 39 15004243
2003 Translational regulation of a novel testis-specific RNF4 transcript. Molecular reproduction and development 39 12874792
2015 RNF4-mediated polyubiquitination regulates the Fanconi anemia/BRCA pathway. The Journal of clinical investigation 36 25751062
2015 Ubiquitin-specific Protease 11 (USP11) Deubiquitinates Hybrid Small Ubiquitin-like Modifier (SUMO)-Ubiquitin Chains to Counteract RING Finger Protein 4 (RNF4). The Journal of biological chemistry 36 25969536
2005 Characterization of cis- and trans-acting elements in the imprinted human SNURF-SNRPN locus. Nucleic acids research 36 16116039
2016 Slx5/Slx8 Promotes Replication Stress Tolerance by Facilitating Mitotic Progression. Cell reports 35 27134171
2009 The SUMO-targeted ubiquitin ligase subunit Slx5 resides in nuclear foci and at sites of DNA breaks. Cell cycle (Georgetown, Tex.) 35 19270524
2007 The yeast Slx5-Slx8 DNA integrity complex displays ubiquitin ligase activity. Cell cycle (Georgetown, Tex.) 35 18032921
2006 Purification of the yeast Slx5-Slx8 protein complex and characterization of its DNA-binding activity. Nucleic acids research 35 17020915
2001 Cooperative coactivation of estrogen receptor alpha in ZR-75 human breast cancer cells by SNURF and TATA-binding protein. The Journal of biological chemistry 35 11696545
2010 Promoter methylation analysis of O6-methylguanine-DNA methyltransferase in glioblastoma: detection by locked nucleic acid based quantitative PCR using an imprinted gene (SNURF) as a reference. BMC cancer 33 20167086
2001 PATZ attenuates the RNF4-mediated enhancement of androgen receptor-dependent transcription. The Journal of biological chemistry 33 11719514
2019 RNF4-A Paradigm for SUMOylation-Mediated Ubiquitination. Proteomics 32 31566917
2024 Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation. Nature structural & molecular biology 31 38649616
2009 Expression of SNURF-SNRPN upstream transcripts and epigenetic regulatory genes during human spermatogenesis. European journal of human genetics : EJHG 31 19471314
2006 Methylation status of imprinting centers for H19/IGF2 and SNURF/SNRPN in primate embryonic stem cells. Stem cells (Dayton, Ohio) 31 17170068
1998 Identification and characterization of a novel RING-finger gene (RNF4) mapping at 4p16.3. Genomics 31 9479498
2015 CDK-mediated RNF4 phosphorylation regulates homologous recombination in S-phase. Nucleic acids research 28 25948581
2013 Role of RNF4 in the ubiquitination of Rta of Epstein-Barr virus. The Journal of biological chemistry 28 23504328
2003 Down-regulation of estrogen receptor beta and transcriptional coregulator SNURF/RNF4 in testicular germ cell cancer. European urology 28 14644130
2000 Rnf4, a RING protein expressed in the developing nervous and reproductive systems, interacts with Gscl, a gene within the DiGeorge critical region. Developmental dynamics : an official publication of the American Association of Anatomists 27 10822263
2007 Slx5 promotes transcriptional silencing and is required for robust growth in the absence of Sir2. Molecular and cellular biology 26 18086879
2015 RNF4 negatively regulates NF-κB signaling by down-regulating TAB2. FEBS letters 25 26299341
2014 Structural analysis of poly-SUMO chain recognition by the RNF4-SIMs domain. The Biochemical journal 25 24844634
2008 Identification of polymorphism and association analysis with reproductive traits in the porcine RNF4 gene. Animal reproduction science 24 18358646
2016 RNF4 regulates DNA double-strand break repair in a cell cycle-dependent manner. Cell cycle (Georgetown, Tex.) 23 26766492
2001 The RING finger protein SNURF is a bifunctional protein possessing DNA binding activity. The Journal of biological chemistry 23 11319220
2013 Small mosaic deletion encompassing the snoRNAs and SNURF-SNRPN results in an atypical Prader-Willi syndrome phenotype. American journal of medical genetics. Part A 22 24311433
2019 SUMOylation by SUMO2 is implicated in the degradation of misfolded ataxin-7 via RNF4 in SCA7 models. Disease models & mechanisms 21 30559154
2014 Disruption of SUMO-targeted ubiquitin ligases Slx5-Slx8/RNF4 alters RecQ-like helicase Sgs1/BLM localization in yeast and human cells. DNA repair 21 25588990
2006 Expression of the Snurf-Snrpn IC transcript in the oocyte and its putative role in the imprinting establishment of the mouse 7C imprinting domain. Journal of human genetics 21 16429232
2017 A CK2-RNF4 interplay coordinates non-canonical SUMOylation and degradation of nuclear receptor FXR. Journal of molecular cell biology 20 28201649
2002 Evidence for translational regulation of the imprinted Snurf-Snrpn locus in mice. Human molecular genetics 19 12075010
2022 RNF4 controls the extent of replication fork reversal to preserve genome stability. Nucleic acids research 18 35640614
2020 Knockdown of endogenous RNF4 exacerbates ischaemia-induced cardiomyocyte apoptosis in mice. Journal of cellular and molecular medicine 18 32722882
2018 Slx5-Slx8 ubiquitin ligase targets active pools of the Yen1 nuclease to limit crossover formation. Nature communications 18 30479332
2017 The Epstein-Barr virus miR-BHRF1-1 targets RNF4 during productive infection to promote the accumulation of SUMO conjugates and the release of infectious virus. PLoS pathogens 17 28414785
2002 Expression of the nuclear RING finger protein SNURF/RNF4 during rat testis development suggests a role in spermatid maturation. Mechanisms of development 17 12351196
2022 DNA damage-induced sumoylation of Sp1 induces its interaction with RNF4 and degradation in S phase to remove 53BP1 from DSBs and permit HR. DNA repair 16 35124373
2016 A post-translational modification cascade employing HDAC9-PIASy-RNF4 axis regulates chondrocyte hypertrophy by modulating Nkx3.2 protein stability. Cellular signalling 16 27312341
2007 Analysis of candidate imprinted genes in PWS subjects with atypical genetics: a possible inactivating mutation in the SNURF/SNRPN minimal promoter. Journal of human genetics 16 17262171
2024 WRN inhibition leads to its chromatin-associated degradation via the PIAS4-RNF4-p97/VCP axis. Nature communications 15 39025847
2020 Regulation of eIF2α by RNF4 Promotes Melanoma Tumorigenesis and Therapy Resistance. The Journal of investigative dermatology 15 32360601
2014 SUMO-targeted ubiquitin ligase RNF4 plays a critical role in preventing chromosome loss. Genes to cells : devoted to molecular & cellular mechanisms 15 25205350
2021 RNF4 Regulates the BLM Helicase in Recovery From Replication Fork Collapse. Frontiers in genetics 14 34868226
2021 RASSF1A disrupts the NOTCH signaling axis via SNURF/RNF4-mediated ubiquitination of HES1. EMBO reports 14 34897944
2017 A novel deletion of SNURF/SNRPN exon 1 in a patient with Prader-Willi-like phenotype. European journal of medical genetics 14 28554868
2021 The E3 Ubiquitin-Protein Ligase RNF4 Promotes TNF-α-Induced Cell Death Triggered by RIPK1. International journal of molecular sciences 13 34071450