Affinage

REV1

Translesion synthesis protein REV1 · UniProt Q9UBZ9

Length
1251 aa
Mass
138.2 kDa
Annotated
2026-06-10
100 papers in source corpus 64 papers cited in narrative 64 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

REV1 is a specialized Y-family DNA polymerase and central scaffolding protein of the translesion synthesis (TLS) machinery that enables cells to tolerate DNA damage at the cost of mutagenesis (PMID:8751446, PMID:12660171, PMID:26680302). As an enzyme, REV1 is a template-independent deoxycytidyl (dCMP) transferase that inserts dCMP opposite template guanine, abasic sites, and a range of N2-guanine minor-groove adducts (PMID:8751446, PMID:10536157, PMID:18591245). Crystal structures of yeast and human ternary complexes establish its unique protein-template mechanism: the templating base (or abasic lesion) is evicted from the helix and incoming dCTP is paired not with the template but with a conserved surrogate arginine (Arg324) (PMID:16195463, PMID:18275815, PMID:19464298). This catalytic activity is biologically relevant chiefly for accurate C insertion opposite endogenous abasic sites and N2-dG adducts (PMID:20980236, PMID:22024240). Beyond catalysis, REV1's principal in vivo role is as a non-catalytic scaffold: its C-terminal four-helix-bundle domain simultaneously binds Rev7 (recruiting Pol-zeta) and the FF-containing RIR/PIP motifs of the insertion polymerases Pol-eta, Pol-iota, and Pol-kappa, thereby coordinating the polymerase switch between insertion and Pol-zeta-mediated extension (PMID:14657033, PMID:19170759, PMID:22859295, PMID:22700975). REV1 is recruited to damage sites through its BRCT domain, which binds PCNA and 5'-phosphorylated recessed primer-template junctions, and through C-terminal ubiquitin-binding motifs (UBMs) that engage monoubiquitinated PCNA and FANCD2 (PMID:16857592, PMID:16982685, PMID:20159559, PMID:26187992). This recruitment couples REV1 to the Rad6/Rad18-PCNA-ubiquitination axis and to the Fanconi anemia pathway during interstrand crosslink repair (PMID:16344468, PMID:22266823, PMID:26071591). REV1 expression and activity are tightly controlled across the cell cycle by peak G2/M abundance, MEC1/ATR-dependent phosphorylation, Hsp90-dependent folding, and APC/REV7-dependent degradation (PMID:16751278, PMID:17035102, PMID:23287467, PMID:21690293). Functionally, REV1 is required for damage-induced point mutagenesis, immunoglobulin gene diversification and class switch recombination, replication through G-quadruplex structures, and the mutagenic filling of PRIMPOL-dependent ssDNA gaps that sustains viability of HR-deficient cancer cells (PMID:12660171, PMID:23140944, PMID:25190518, PMID:34508659).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1996 High

    Established REV1's defining biochemical activity, answering what reaction the protein catalyzes: a template-dependent dCMP transferase that acts opposite abasic sites and guanine.

    Evidence In vitro dCMP transferase assay with purified yeast Rev1; conserved in purified human REV1

    PMID:10536157 PMID:8751446

    Open questions at the time
    • Did not establish the structural basis for dCMP selectivity
    • In vivo relevance of catalysis versus scaffolding unresolved
  2. 2002 High

    Quantitative kinetics defined REV1 as a low-processivity, G-template-specific polymerase, clarifying that its insertion fidelity is biased toward C opposite G rather than broad lesion bypass.

    Evidence Steady-state kinetics of nucleotide incorporation by purified yeast Rev1 across multiple substrates

    PMID:11850424

    Open questions at the time
    • Did not resolve whether catalytic insertion or structural roles dominate at abasic sites in vivo
  3. 2001 High

    Genetic and reconstitution analysis indicated that at abasic sites REV1's predominant contribution is structural rather than catalytic, reframing it as a pathway organizer alongside Pol-delta and Pol-zeta.

    Evidence Genetic epistasis plus in vitro reconstitution and kinetics in yeast

    PMID:11316789

    Open questions at the time
    • Structural role mechanism not defined at molecular level
    • Did not identify the scaffolding interactions later mapped
  4. 2003 High

    Identified REV1's scaffolding function by showing its C-terminus binds Pol-eta, -iota, -kappa, and Rev7 (Pol-zeta) at a common ~100-residue region, with Rev7 competing for binding — establishing REV1 as a polymerase-bridging hub.

    Evidence Co-IP, GST pulldown, and competition assays with mouse Rev1 and partner polymerases; loss-of-function in DT40 cells

    PMID:12660171 PMID:14657033

    Open questions at the time
    • Structural basis of simultaneous versus competitive binding not yet resolved
    • Recruitment to lesions not yet mechanistically defined
  5. 2005 High

    Defined the recruitment logic: REV1's BRCT domain and C-terminal UBMs bind PCNA and monoubiquitinated PCNA respectively, coupling REV1 to the Rad18/PCNA-ubiquitination damage signal and to replication foci.

    Evidence Domain mutagenesis, Co-IP, foci imaging in DT40/mouse cells; in vitro TLS activation by ubiquitinated PCNA

    PMID:15741181 PMID:16344468 PMID:16857592 PMID:16982685

    Open questions at the time
    • Temporal coordination of BRCT versus UBM recruitment not resolved
    • Structural detail of UBM-ubiquitin contact pending
  6. 2005 High

    Crystal structures of yeast Rev1 ternary complexes solved the central mechanistic question of nucleotide selection, revealing a protein-template mechanism with base eviction and Arg324 as a surrogate templating residue.

    Evidence X-ray crystallography of Rev1-DNA-dCTP ternary complexes at template G and abasic sites; conserved in human REV1 structure

    PMID:16195463 PMID:18275815 PMID:19464298 PMID:21167175

    Open questions at the time
    • Did not address how scaffolding CTD is positioned relative to catalytic core
    • Conformational dynamics during base eviction not captured
  7. 2007 High

    Pinpointed the UBM-ubiquitin interface by mutagenesis and NMR, showing UBM contacts are required for damage-induced mutagenesis and survival, linking ubiquitin recognition to function.

    Evidence Mutational analysis, in vitro binding to ubiquitinated PCNA, NMR of UBM-ubiquitin complex, in vivo mutagenesis in yeast

    PMID:17517887 PMID:20159559

    Open questions at the time
    • How UBM engagement is regulated during the cell cycle not defined
  8. 2009 High

    Defined the FF-containing RIR motif as the shared docking signal in Pol-eta/-iota/-kappa for the REV1 CTD, explaining how REV1 selects insertion polymerases.

    Evidence Yeast two-hybrid, pulldown, sequence alignment, and complementation in Polk-null MEFs

    PMID:19170759

    Open questions at the time
    • Did not establish how competing RIR/Rev7 interactions are temporally ordered
  9. 2012 High

    Structures of the REV1 CTD four-helix bundle in complex with Rev7/Rev3 and RIR peptides resolved how REV1 simultaneously engages extension (Pol-zeta) and insertion polymerases via two distinct surfaces, providing the molecular basis for polymerase switching.

    Evidence X-ray crystallography of quaternary and ternary complexes and NMR of mouse/human CTD with conformational dynamics analysis

    PMID:22303021 PMID:22691049 PMID:22700975 PMID:22859295 PMID:22859296

    Open questions at the time
    • Order of polymerase exchange events not directly observed
    • Coupling of CTD switching to the catalytic core unresolved
  10. 2012 High

    Connected REV1 to the Fanconi anemia pathway and to antibody diversification, showing FAAP20 binds monoubiquitinated Rev1 and that Rev1 scaffolds Ung to switch regions during class switch recombination independent of catalysis.

    Evidence Co-IP, ChIP, domain analysis, foci imaging, and catalytic-mutant rescue in B cells

    PMID:22266823 PMID:23140944

    Open questions at the time
    • Mechanistic link between FA recruitment and TLS polymerase loading incompletely defined
  11. 2016 High

    Refined the polymerase-switch model by showing PolD3-RIR binds the REV1 CTD with high affinity and that PIP and RIR motifs have overlapping specificities, suggesting how the full Pol-zeta extender is assembled after inserter displacement.

    Evidence NMR structures and binding-affinity measurements of Rev1-CT complexes with PolD3-RIR and PIP/RIR peptides

    PMID:26903512 PMID:26982350 PMID:27001510

    Open questions at the time
    • Kinetics of switch in the context of an active replication fork not measured
  12. 2016 High

    Established that human REV1 is an indispensable scaffold for Y-family-mediated, predominantly error-free TLS opposite UV lesions, distinguishing its mammalian function from yeast and from Pol-zeta-dependent bypass.

    Evidence Systematic siRNA/knockout of multiple polymerases with single-lesion plasmid TLS assays in human and mouse cells

    PMID:26680302

    Open questions at the time
    • Relative contribution of catalytic versus scaffolding REV1 to error-free bypass not fully partitioned
  13. 2014 Medium

    Revealed a replication-fidelity role beyond classical lesion bypass: REV1 is required to replicate G-quadruplex DNA, and its loss causes stochastic loss of parental histone marks and epigenetic instability.

    Evidence In vitro G4 unfolding/binding assays and DT40 knockout with ChIP for histone marks and G4-motif mutagenesis

    PMID:24366879 PMID:25190518

    Open questions at the time
    • Whether G4 resolution at forks uses the same recruitment machinery as lesion TLS is unclear
    • Single-locus mechanism not generalized genome-wide
  14. 2021 High

    Positioned REV1-Pol-zeta in the mutagenic filling of PRIMPOL-dependent ssDNA gaps, defining a vulnerability of HR-deficient cancer cells and validating a small-molecule inhibitor (JH-RE-06).

    Evidence DNA fiber, electron microscopy, knockdown, inhibitor treatment, and xenograft models

    PMID:34508659

    Open questions at the time
    • Substrate scope of gap-filling beyond BRCA-deficient context not defined
    • How gap-filling integrates with cell-cycle-regulated REV1 abundance unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How REV1's catalytic core, its scaffolding CTD, and its multiple regulatory inputs (phosphorylation, SUMOylation, Hsp90 folding, APC/REV7 degradation) are integrated into a single dynamic complex that orders insertion, switching, and extension at a fork remains unresolved.
  • No structure of full-length REV1 with both catalytic and scaffolding modules engaged
  • Quantitative timing of polymerase exchange in vivo not measured
  • Regulatory crosstalk among PTMs not mechanistically connected

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140097 catalytic activity, acting on DNA 8 GO:0060090 molecular adaptor activity 5 GO:0003677 DNA binding 4 GO:0016740 transferase activity 4
Localization
GO:0005634 nucleus 4 GO:0005694 chromosome 2
Pathway
R-HSA-1643685 Disease 4 R-HSA-73894 DNA Repair 4 R-HSA-69306 DNA Replication 3
Partners
FAAP20FANCD2PCNAPOLHPOLIPOLKREV3REV7
Complex memberships
REV1-Pol-zeta (REV1-REV7-REV3) translesion polymerase complexREV1-REV7 heterodimer

Evidence

Reading pass · 64 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1996 Yeast Rev1 protein has a deoxycytidyl transferase (dCMP transferase) activity, transferring a dCMP residue from dCTP to the 3' end of a DNA primer in a template-dependent reaction. Efficient transfer occurs opposite a template abasic site, and approximately 20% transfer occurs opposite template guanine. The inserted cytosine opposite an abasic site produces a terminus efficiently extended by Pol-zeta but not Pol-alpha. In vitro biochemical assay (dCMP transferase activity assay with purified protein) Nature High 8751446
1999 Human REV1 protein is a dCMP transferase that specifically inserts a dCMP residue opposite a template G, and efficiently inserts dCMP opposite template AP (apurinic/apyrimidinic) sites and uracil residues. In vitro biochemical assay with purified recombinant human REV1 protein Nucleic Acids Research High 10536157
2001 Yeast Rev1 is a G template-specific DNA polymerase: it specifically inserts a C residue opposite template G, and is approximately 25-, 40-, and 400-fold less efficient at inserting C opposite an abasic site, O6-methylguanine, and 8-oxoguanine, respectively. Rev1 has low processivity (~1.6 nucleotides per binding event). Steady-state kinetic analysis of nucleotide incorporation by purified yeast Rev1 Journal of Biological Chemistry High 11850424
2001 In AP site bypass, Rev1's predominant role is likely structural rather than catalytic insertion; the combined action of Pol-delta (inserting A opposite AP site) and Pol-zeta (extending) constitutes the major bypass pathway, with Rev1 and Pol-eta contributing to insertion of other nucleotides at lower frequency. Genetic epistasis analysis combined with in vitro reconstitution and steady-state kinetics Genes & Development High 11316789
2001 The deoxycytidyl transferase activity of human REV1 is associated with its conserved polymerase domain; deletion and point mutation analysis demonstrated that the domain required for dCMP transferase and DNA binding activities resides within the conserved Y-family polymerase domain. Deletion and point mutation analysis of human REV1S protein, in vitro transferase assay Journal of Biological Chemistry High 11278384
2002 Human REV1 is required for UV-induced mutagenesis; cells with reduced hREV1 mRNA via antisense expression show significantly lower frequencies of UV-induced 6-thioguanine-resistant mutants without affecting cytotoxic sensitivity. Antisense RNA knockdown in human cells, clonogenic survival and mutation frequency assays PNAS Medium 10760286
2003 Mouse Rev1 physically associates with Pol-kappa, Pol-iota, Pol-eta, and Rev7 (subunit of Pol-zeta), and each of these proteins binds to the same ~100 amino acid C-terminal region of Rev1. Rev7 competes directly with Pol-kappa for binding to the Rev1 C-terminus. The DNA polymerase activity of Rev1 and Pol-kappa is unaffected when they form a complex. Co-immunoprecipitation, GST pulldown, in vitro primer extension assay EMBO Journal High 14657033
2003 Rev1-deficient DT40 cells grow slowly, are sensitive to a wide range of DNA-damaging agents, and show markedly reduced non-templated immunoglobulin gene mutation (translesion bypass). UV exposure causes chromosome breakage in Rev1-deficient cells. Homologous recombination (sister chromatid exchange and gene conversion) is unaffected. Gene disruption in DT40 cells, clonogenic survival, SCE assay, Ig gene mutation analysis, chromosome analysis EMBO Journal High 12660171
2003 Human REV1 and REV7 proteins form a stable heterodimer in solution. REV7 does not influence the stability, substrate specificity, or kinetic parameters of the transferase reactions of REV1 in contrast to the stimulatory effect of yeast Rev7 on Rev3. Purification of REV1-REV7 complex, biochemical analysis of transferase kinetics Journal of Biological Chemistry Medium 12529368
2004 Rev1 efficiently incorporates C opposite the N2-propano-2'-deoxyguanosine (gamma-HOPdG) minor-groove adduct, from which Pol-zeta subsequently extends, completing error-free bypass. This supports a role for Rev1's DNA synthetic activity in bypassing N2-guanine minor-groove adducts by not contacting the DNA minor groove. In vitro primer extension assay with purified yeast Rev1 and Pol-zeta on adduct-containing templates Molecular and Cellular Biology High 15282292
2005 Crystal structure of yeast Rev1 bound to template G and incoming dCTP reveals that the polymerase dictates nucleotide identity via a protein-template mechanism: template G is evicted from the DNA helix and makes hydrogen bonds with a segment of Rev1, while incoming dCTP pairs with an arginine residue (Arg324) rather than the templating base. X-ray crystallography (crystal structure of ternary complex) Science High 16195463
2005 Vertebrate DNA damage tolerance requires the C-terminus of REV1, which mediates interactions with PCNA, but does not require the BRCT domain or the catalytic transferase activity. A region adjacent to the polymerase-interacting domain mediates interaction with PCNA. Domain deletion/mutation analysis in avian DT40 cells, survival assays, co-immunoprecipitation Nucleic Acids Research High 15741181
2005 Ubiquitinated PCNA (monoubiquitinated at Lys-164 by Rad6/Rad18) specifically activates Rev1 (and Pol-eta) for translesion synthesis in vitro, while DNA polymerase activity of Pol-zeta remains unaffected by PCNA ubiquitination. Robust in vitro ubiquitination system for yeast PCNA, in vitro TLS assay with ubiquitinated PCNA PNAS High 16344468
2005 The yeast Rev1 PAD (polymerase-associated domain) mediates binding to Rev7 (accessory subunit of Pol-zeta), forming a stable Rev1-Rev7 complex that co-purifies. This reveals a novel role for the PAD in protein-protein interactions. Co-purification, co-immunoprecipitation from yeast Molecular and Cellular Biology Medium 16227619
2006 Rev1 physically associates with Pol-zeta through its C-terminus binding to the Rev3 polymerase domain. A rev1 mutant lacking the C-terminal 72 residues (inactivating Rev3 interaction) shows the same UV sensitivity and loss of UV-induced mutagenesis as rev1Δ. Rev1 binding to Pol-zeta enhances Pol-zeta's proficiency for extending mismatched primer termini and primers opposite DNA lesions. Yeast two-hybrid, co-immunoprecipitation from yeast, in vitro primer extension assay, UV survival and mutagenesis assays Molecular and Cellular Biology High 17030609
2006 Mouse REV1 binds directly to PCNA, and monoubiquitylation of PCNA enhances this interaction. The BRCT domain of REV1 is required for this interaction and for targeting REV1 to replication foci in unirradiated cells. Deletion or mutational inactivation of the BRCT domain abolishes REV1 targeting to replication foci in unirradiated cells (but not UV-irradiated cells) and is required for cell survival and DNA damage-induced mutagenesis in vivo. Co-immunoprecipitation, live-cell imaging of replication foci, genetic complementation in DT40 cells and yeast Molecular Cell High 16857592
2006 Mouse REV1 physically interacts with ubiquitin via ubiquitin-binding motifs (UBMs) located at the C-terminus of REV1. These UBMs mediate enhanced association between monoubiquitylated PCNA and REV1 and are required for UV-induced REV1 localization to replication foci, DNA damage tolerance, and damage-induced mutagenesis in vivo. Yeast two-hybrid, GST pulldown assay, cellular localization (foci assay), survival and mutagenesis assays Molecular and Cellular Biology High 16982685
2006 S. cerevisiae Rev1 protein levels are subject to cell cycle control, being approximately 50-fold higher in G2/M than in G1/S phase. This cell cycle regulation is physiologically relevant: a rev1Δ strain shows differential survival after UV irradiation depending on the cell cycle stage at which UV is applied. Protein level measurement by western blot across cell cycle stages, UV survival assays at defined cell cycle stages PNAS High 16751278
2006 Yeast Rev1 is a phosphoprotein whose phosphorylation level is cell cycle regulated (unphosphorylated in G1, phosphorylated in S phase, hyper-phosphorylated in mitosis) and is further hyper-phosphorylated in response to DNA damage. Rev1 binds to chromosomes throughout the cell cycle in a MEC1-dependent manner, and phosphorylation does not modulate chromosome binding. Western blot (phosphoprotein analysis), chromosome spreading technique, genetic analysis with mec1 mutant DNA Repair Medium 17035102
2006 Rev1 is required for efficient UV-induced mutagenesis in human cells; ribozyme-mediated reduction of REV1 mRNA (up to 90%) reduces UV-induced HPRT mutant frequency by up to 75% without affecting cytotoxic UV sensitivity. Ribozyme knockdown of REV1 mRNA in human cells, clonogenic survival, mutation frequency at HPRT locus Nucleic Acids Research Medium 12930947
2006 The yeast Rev1 C-terminus mediates interactions with Rev7 via both the PAD region and the extreme C-terminus and BRCT region. Elevated levels of the Rev1 C-terminus confer a dominant-negative effect on viability and induced mutagenesis after DNA damage, dependent on REV7. Co-immunoprecipitation from yeast crude extracts, dominant-negative overexpression assay, genetic analysis Molecular and Cellular Biology Medium 16923957
2007 A ubiquitin-binding motif in the C-terminus of yeast Rev1 (residues L821, P822, I825) mediates functional interaction with the ubiquitin moiety of monoubiquitinated PCNA. Point mutations L821A,P822A,I825A abolish this interaction in vitro and strongly attenuate damage-induced mutagenesis in vivo. Mutational analysis of Rev1, in vitro binding assay with ubiquitinated PCNA, in vivo mutagenesis assay Journal of Biological Chemistry High 17517887
2007 Human REV1 accumulates at UV-irradiated areas of nuclei in cells expressing Pol-eta but not in Pol-eta-deficient XP-V cells. The Pol-eta–REV1 interaction (via FF residues in Pol-eta) is required for REV1 nuclear accumulation at UV damage sites and for suppression of spontaneous mutations, but not for accurate TLS of UV lesions. Immunofluorescence, complementation of XP-V cells with Pol-eta mutants, mutation frequency assay DNA Repair High 19157994
2008 TLS at stalled replication forks in DT40 cells requires both the translesion polymerase-interaction domain and ubiquitin-binding domain in the REV1 C-terminus. PCNA ubiquitination is not required for maintaining normal fork progression on damaged DNA but is essential for filling postreplicative gaps. Thus, PCNA ubiquitination and REV1 play distinct, temporally separated roles in DNA damage bypass. Gene disruption and domain deletion/mutation analysis in DT40 cells, DNA fiber assay for fork progression, genetic epistasis Molecular Cell High 18498753
2008 The Fanconi anemia (FA) core complex is required for efficient assembly of Rev1 nuclear foci (independently of FANCD2, FANCI, and PCNA monoubiquitination) and for efficient spontaneous and UV-induced point mutagenesis. Rev1 is required downstream of the FA core complex to prevent chromosomal aberrations caused by DNA crosslinkers. Complementation analysis in FA-deficient cells, immunofluorescence for Rev1 foci, mutation frequency assay, chromosomal aberration analysis DNA Repair Medium 18448394
2008 Yeast Rev1 protein interacts with yeast Pol-eta through the Rev1 polymerase-associated domain (PAD), not the C-terminus as in humans. This complex formation enhances the DNA synthesis activity of Rev1. Co-immunoprecipitation, in vitro DNA synthesis assay, domain deletion analysis Molecular and Cellular Biology Medium 17875922
2008 Human REV1 inserts dCTP with similar catalytic efficiency (kcat/Km) opposite template G and N2-alkylguanine adducts of increasing bulk (up to N2-BPG) but is severely inhibited by O6-alkylguanine adducts. REV1 binds N2-BPG-adducted DNA ~3-fold more tightly than unmodified G-containing DNA, and the rate-limiting step is likely product release rather than chemistry opposite N2-BPG. Steady-state and pre-steady-state kinetics, elemental effect assay with purified recombinant human REV1 Journal of Biological Chemistry High 18591245
2008 Crystal structure of yeast Rev1 in ternary complex with DNA containing an abasic (AP) lesion and dCTP reveals that the abasic lesion is driven to an extrahelical position (similar to template G) and C incorporation is mediated by the conserved Arg324 (surrogate arginine), demonstrating the same protein-template mechanism for AP site bypass. X-ray crystallography of ternary Rev1-DNA(AP)-dCTP complex Structure High 18275815
2008 The Rev1 BRCT domain of mammalian cells plays a role in early (S-phase-associated) mutagenic TLS: cells with a BRCT domain deletion are deficient in the early DNA damage bypass pathway and show reduced UV-induced mutagenesis. Rev1-deficient cells show defects in both early and late bypass pathways, with persistent gaps causing G2 arrest. Targeted deletion of BRCT domain in mouse embryonic fibroblasts, DNA replication analysis, cell cycle analysis Molecular and Cellular Biology High 19332561
2009 A novel REV1-interacting region (RIR) motif containing two consecutive phenylalanines (FF motif, consensus x-x-x-F-F-y-y-y-y) present in Pol-kappa, Pol-iota, and Pol-eta is essential for binding to REV1 C-terminus. A Pol-kappa mutant lacking REV1-binding activity fails to complement genotoxin sensitivity of Polk-null cells. Yeast two-hybrid, pulldown assay, sequence alignment, functional complementation in Polk-null MEFs Genes to Cells High 19170759
2009 Yeast Rev1 promotes complex formation of Pol-zeta with the Pol32 subunit of Pol-delta: Pol32 physically interacts with Rev1 but does not directly bind Pol-zeta, and can bind the Rev1-Pol-zeta complex through Rev1. Pol32 binding does not stimulate DNA synthesis by Rev1 or Pol-zeta in this complex. Co-immunoprecipitation, in vitro pull-down, in vitro DNA synthesis assay with reconstituted complexes PNAS Medium 19487673
2009 Crystal structure of human REV1 ternary complex reveals conservation of the protein-template mechanism from yeast: template G is evicted, incoming dCTP pairs with surrogate arginine. Human REV1 has two unique inserts (I1 and I2): I1 extends >20 Å from the active site (potential platform for protein-protein interactions) and I2 acts as a flap on the hydrophobic pocket accommodating template G. X-ray crystallography of human REV1 ternary complex Journal of Molecular Biology High 19464298
2009 Mec1 (ATR homolog) promotes association of the Pol-zeta/Rev1 complex with double-strand breaks (DSBs) through Mec1-dependent phosphorylation. Rev1 plays a non-catalytic role in this DSB association. Rev1 association with DSBs does not require Rad24, nor Rad6-Rad18-mediated PCNA ubiquitination. Chromatin immunoprecipitation (ChIP) at defined DSBs in yeast, genetic analysis Current Biology Medium 16546083
2009 Crystal structure of human REV7 in complex with a REV3 fragment reveals the mechanism of REV7-REV3 interaction. The REV7-REV3 structural interface creates a binding site for REV1, explaining how REV7 acts as an adaptor protein to recruit Pol-zeta to lesion sites. X-ray crystallography of REV7-REV3 fragment complex, biochemical interaction analysis Journal of Biological Chemistry High 20164194
2009 Human Rev1 disrupts G-quadruplex (G4) DNA structures in vitro and prevents refolding. hRev1 binds G4 DNA substrates with 4-15-fold lower Kd than non-G4 DNA. Nucleotidyl transfer activity is not necessary for G4 unfolding. Rev1 can promote fork progression by dislodging tetrad guanines or preventing refolding. Fluorescence-based G4 disruption assay, binding affinity measurement (Kd), pre-steady-state kinetics Nucleic Acids Research High 24366879
2010 The UBM of S. cerevisiae Rev1 (second UBM) binds ubiquitin at the hydrophobic surface centered at L8 (not I44) of ubiquitin. Only the second UBM of yeast Rev1 is functional and is essential for Rev1-dependent cell survival and mutagenesis. Mutations disrupting UBM-ubiquitin interaction impair Rev1-mediated DNA damage tolerance in vivo. NMR structure of UBM-ubiquitin complex, mutagenesis of ubiquitin binding interface, in vivo mutagenesis and survival assays Molecular Cell High 20159559
2010 Rev1's DNA polymerase catalytic activity is biologically significant in vivo for resistance to 4-NQO (which causes N2-dG adducts) but not to UV or cisplatin. The catalytic activity is more critical when error-free tolerance (Mms2 pathway) is disrupted. Catalytic dead Rev1 mutant in yeast, survival assays, mutation frequency assays with defined genotoxins Genetics High 20980236
2010 Crystal structure of yeast Rev1 in ternary complex with AP site-containing DNA and dCTP shows the abasic lesion in extrahelical position with C incorporation mediated by conserved Arg324, establishing a unified protein-template mechanism for both template G and AP site bypass. X-ray crystallography of ternary complex Journal of Molecular Biology High 21167175
2011 The Rev1-PCNA interaction occurs at a non-canonical binding site near the monomer-monomer interface of the PCNA trimer (via the Rev1 PAD domain), which is the same novel interface used by Pol-zeta. The pol30-113 mutation at this interface specifically disrupts the Rev1/Pol-zeta-dependent TLS pathway. GST pull-down, affinity bead pull-down, gel filtration, genetic epistasis analysis Journal of Biological Chemistry Medium 21799021
2011 The BRCT region of Rev1 (via an extra α-helix N-terminal to the BRCT domain) specifically binds 5'-phosphorylated recessed primer-template junctions. Rev1 also binds recessed 3' primer-template junctions. These dual DNA binding characteristics are proposed to mediate recruitment of Rev1 downstream of stalled forks. In vitro DNA binding assays with purified mouse and yeast Rev1, domain deletions DNA Repair Medium 21752727
2011 The dCMP transferase catalytic activity of Rev1 is biologically relevant and required specifically for dCMP insertion during bypass of endogenously generated AP sites (from uracil excision) in yeast chromosomal DNA. Loss of Rev1 catalytic activity allows insertion of dGMP or dTMP, indicating that Rev1 catalysis is the primary determinant of C insertion opposite AP sites in vivo. Frameshift- and nonsense-reversion assays in yeast using catalytic-dead Rev1 mutant DNA Repair High 22024240
2011 Human REV1, REV3, and REV7 interact in vivo (by co-immunoprecipitation) and together promote homologous recombination repair of DSBs. REV1, REV3, or REV7-depleted cells show increased chromosomal aberrations, residual DSBs, and defects in HR repair after ionizing radiation. Pol-eta and RAD18-dependent PCNA monoubiquitination are not required for this DSB repair function. Co-immunoprecipitation, siRNA knockdown, chromosomal aberration analysis, γH2AX foci, HR assay Nucleic Acids Research Medium 21926160
2012 FAAP20, an integral subunit of the FA core complex, contains a UBZ4 domain that binds monoubiquitinated Rev1. FAAP20 binding stabilizes Rev1 nuclear foci and promotes interaction of the FA core with PCNA-Rev1 DNA damage bypass complexes. This provides a mechanistic link between the FA core complex and TLS polymerase activity. Identification of FAAP20 as FA core component, co-immunoprecipitation, domain analysis, immunofluorescence for Rev1 foci Nature Structural & Molecular Biology High 22266823
2012 Crystal structure of the quaternary translesion polymerase complex consisting of the Rev1 CTD, heterodimeric Pol-zeta (Rev7/Rev3-RBM), and Pol-kappa RIR reveals that the Rev1 CTD serves as a scaffold with two distinct surfaces: one for Rev7 binding (at α2-α3 loop and α3) and one for RIR binding (creating a hydrophobic cavity for FF residues). This provides the molecular basis for Rev1 as a scaffold bridging insertion and extension polymerases. Protein purification, X-ray crystallography, yeast two-hybrid to validate interface residues Journal of Biological Chemistry High 22859295
2012 Crystal structure of human REV1 CTD-REV7-REV3 fragment ternary complex reveals that the Rev1 CTD adopts a four-helix bundle interacting with REV7 via a conserved linker between helices 2 and 3, at a site distinct from the Pol-eta/kappa binding site. REV7 acts as an adaptor recruiting Pol-zeta to stalled forks via Rev1-REV7 interaction. X-ray crystallography, co-immunoprecipitation, cellular survival assays Journal of Biological Chemistry High 22859296
2012 NMR solution structure of mouse Rev1 CTD reveals an atypical four-helix bundle. Two distinct surfaces of the Rev1 CTD mediate Rev7 binding (centered at α2-α3 loop and N-terminal α3) and Pol-kappa RIR binding (FF residues binding in a deep hydrophobic cavity). Binding of Pol-kappa RIR induces folding of the disordered RIR into a three-turn α-helix and induces formation of a β-hairpin in the Rev1 CTD N-terminal loop. NMR solution structure determination, yeast two-hybrid, binding affinity measurements Journal of Biological Chemistry High 22700975
2012 NMR structure of human Rev1 CTD and its complex with Pol-eta RIR shows the four-helix bundle with β-hairpin; free Rev1-CT and the Rev1-CT/Pol-eta-RIR complex exhibit μs-ms conformational dynamics at the RIR binding site that may facilitate polymerase exchange. NMR structure determination, NMR spin-relaxation and relaxation dispersion measurements Biochemistry High 22691049
2012 Rev1 directly interacts with Ung (uracil DNA glycosylase) and targets Ung to switch regions during class switch recombination (CSR) in an AID-dependent manner. Rev1-deficient B cells show reduced Ung recruitment to S regions, decreased DNA-dU glycosylation, and reduced CSR. Rescue of CSR by catalytically inactive Rev1 shows this function is mediated by Rev1 scaffolding, not enzymatic activity. Co-immunoprecipitation, ChIP, CSR assays in Rev1-/- B cells, complementation with catalytic mutant Cell Reports High 23140944
2012 Rev1 is required for efficient point mutagenesis (insertion/deletion) at BPDE-dG in mammalian cells, specifically for mutagenic TLS. The Rev1 C-terminus is required; yeast and mammalian three-hybrid assays confirmed REV7 mediates REV3-Rev1 C-terminus interaction, supporting Rev1 recruiting Pol-zeta through REV7. Single lesion plasmid TLS assay in Rev1-/- MEFs, complementation with C-terminal deletion mutant, yeast/mammalian three-hybrid assay Journal of Biological Chemistry High 22303021
2012 REV7 is required for APC/CDH1- and APC/CDC20-mediated polyubiquitination and proteasomal degradation of REV1. REV7 binds to an N-terminal region of REV1 that contains both the APC degron and an additional REV7-binding domain. Depletion of REV7 stabilizes REV1 by preventing polyubiquitination. Co-immunoprecipitation, ubiquitination assay, siRNA knockdown, overexpression of APC co-activators Cell Cycle Medium 23287467
2013 NMR mapping shows that the Rev1 BRCT domain of S. cerevisiae directly interacts with PCNA in solution. The PCNA-binding interface of the Rev1-BRCT domain comprises conserved residues of the outer α1-helix surface and flanking loops. Rev1-BRCT binds the inter-domain region of PCNA that overlaps with the PIP-box binding site; Rev1-BRCT and Pol-eta PIP-box interactions with the same PCNA monomer are mutually exclusive. Solution NMR structure of Rev1-BRCT, TROSY NMR binding analysis with 87 kDa PCNA, competition with PIP-box peptide Journal of Molecular Biology High 23747975
2014 REV1-deficient cells are compromised in replicating G-quadruplex (G4)-forming DNA, leading to stochastic loss of parental histone marks (H3K4me3, H3K9/14ac) and changes in gene expression. A single G4 motif on the leading strand template is responsible for this epigenetic instability at the BU-1 locus, with effects depending on the G4's distance from the TSS. Gene disruption in DT40 cells, ChIP for histone marks, gene expression analysis, G4 motif mutagenesis EMBO Journal Medium 25190518
2015 Rev1-Pol-zeta complex recruitment to interstrand crosslink (ICL) sites requires the Fanconi anemia core complex but not FancI-FancD2. The extension step of ICL bypass involves a Rev1-Pol-zeta complex. Deep sequencing shows ICL repair introduces a short mutagenic tract with ~1% maximum mutation frequency. Site-specific ICL in Xenopus egg extracts, deep sequencing of repair products, immunodepletion experiments EMBO Journal High 26071591
2015 Rev1 is targeted to laser-induced DNA damage in a manner dependent on its ubiquitin-binding motifs (UBMs), RAD18, and monoubiquitinated FANCD2 (FANCD2-mUb) which associates with REV1. Expression of FANCD2-Ub chimera in RAD18-depleted cells enhances REV1 assembly at damage sites. REV1 also protects nascent replication tracts from degradation by stabilizing RAD51 filaments. Laser microirradiation and immunofluorescence, co-immunoprecipitation, DNA fiber assay Nucleic Acids Research Medium 26187992
2015 In human and mouse cells, Rev1 is indispensable for TLS mediated by Pol-eta, Pol-iota, and Pol-kappa opposite UV lesions, but is not required for Pol-zeta-dependent TLS. Rev1 promotes predominantly error-free TLS opposite UV lesions in humans, acting as an indispensable scaffolding component for Y-family polymerases. siRNA/gene knockout in human and mouse fibroblasts, single lesion plasmid TLS assay Genes & Development High 26680302
2016 PolD3, a subunit of the four-subunit Pol-zeta complex, contains an RIR motif whose interaction with Rev1-CT is among the tightest mediated by RIR motifs. NMR structure of Rev1-CT/PolD3-RIR complex reveals structural basis for high affinity. This suggests a mechanism for polymerase switching: PolD3-RIR binding to Rev1-CT displaces inserter polymerases and facilitates assembly of the full Pol-zeta extender complex. NMR structure determination of Rev1-CT/PolD3-RIR complex, binding affinity measurements Biochemistry High 26982350
2016 Budding yeast Rad5 physically interacts with Rev1 via an RFF motif in the Rad5 N-terminus that binds a hydrophobic pocket within the Rev1 CTD. Crystal structure of the Rad5-Rev1 interaction was determined. Rad5's TLS function (via Rev1 recruitment) and its error-free DDT function (via E3 ligase) are separable. Disrupting the Rev1-Rad5 interaction inactivates Rev1's non-catalytic TLS function. Domain mapping, crystal structure of Rad5-Rev1 interaction, genetic separation-of-function analysis Nucleic Acids Research High 27001510
2016 REV1 promotes PCNA monoubiquitylation after UV radiation by interacting with ubiquitylated RAD18, which facilitates release of non-ubiquitylated RAD18 from ubiquitylated RAD18 trapping and allows RAD18 chromatin recruitment. This stimulatory effect also occurs after hydroxyurea and mitomycin C treatment but not MMS. Co-immunoprecipitation, PCNA ubiquitylation assay, chromatin fractionation Journal of Cell Science Medium 26795561
2016 The PIP motif of yeast Pol-eta mediates interactions with both PCNA and Rev1 CTD, binding in the hydrophobic pocket of the Rev1 C-terminal domain. RIR motifs of human Pol-kappa and PIP motifs of yeast Msh6 can also bind both PCNA and Rev1, demonstrating overlapping specificities of PIP and RIR motifs. Binding assays, fluorescence polarization, yeast two-hybrid, mutagenesis Journal of Biological Chemistry Medium 26903512
2018 Rev7 dimerizes (homodimer) when tethered by the two Rev7-binding motifs (RBMs) in Rev3, using the conventional HORMA dimerization interface. The Rev7 dimer can bind only one copy of Rev1, revealing an unexpected 1:2:1 Rev1:Rev7:Rev3 architecture. Rev7 also heterodimerizes with Mad2 and p31comet. Mutation of the Rev7 dimer interface increases cellular sensitivity to DNA damage. In vitro structural analysis, biochemical interaction studies, functional assay in Rev7-/- cells PNAS High 30111544
2015 Starvation induces SUMO2/3 modification (SUMOylation) of REV1, which relieves REV1's inhibition of p53, enhancing p53-dependent proapoptotic gene expression and apoptosis in cancer cells. REV1 is a novel binding partner of tumor suppressor p53 and regulates its activity. Co-immunoprecipitation (REV1-p53 interaction), SUMOylation assay, gene expression analysis, apoptosis assay Cancer Research Medium 25614517
2011 Hsp90 binds REV1 in vivo and in vitro. Inhibition of Hsp90 reduces REV1 protein levels through proteasomal degradation, suppresses UV-induced mutagenesis, disrupts the interaction between REV1 and monoubiquitinated PCNA, and suppresses UV-induced REV1 focus formation. Hsp90 promotes correct folding of REV1 to enable its interaction with monoubiquitinated PCNA. Co-immunoprecipitation (in vivo and in vitro), Hsp90 inhibitor treatment, immunofluorescence for foci, mutagenesis assay Molecular and Cellular Biology Medium 21690293
2021 REV1-Pol-zeta (inhibited by small molecule JH-RE-06) maintains viability of BRCA1/2-deficient cells through mutagenic repair of PRIMPOL-dependent ssDNA gaps. Gap accumulation requires SMUG1 glycosylase and is exacerbated by RAD18 depletion or REV1-Pol-zeta inhibition. JH-RE-06 is preferentially toxic toward HR-deficient cancer cells and acts additively with crosslinking agents or PARP inhibitors. DNA fiber analysis, electron microscopy, small molecule inhibitor (JH-RE-06), siRNA knockdown, mouse xenograft models Molecular Cell High 34508659
2006 Human REV1 has a higher affinity for single-stranded DNA (ssDNA) than for primer termini, targeting it to included primer termini. This property requires the N- and C-terminal domains (the catalytic core alone loses this function). This distinguishes REV1 from other DNA polymerases including Pol-alpha, Pol-beta, and Pol-eta. In vitro DNA binding assays with purified human REV1 and deletion mutants Journal of Biological Chemistry Medium 16803901

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1996 Deoxycytidyl transferase activity of yeast REV1 protein. Nature 504 8751446
2003 Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis. The EMBO journal 303 14657033
2001 Roles of yeast DNA polymerases delta and zeta and of Rev1 in the bypass of abasic sites. Genes & development 300 11316789
2008 PCNA ubiquitination and REV1 define temporally distinct mechanisms for controlling translesion synthesis in the avian cell line DT40. Molecular cell 216 18498753
2005 Rev1 employs a novel mechanism of DNA synthesis using a protein template. Science (New York, N.Y.) 208 16195463
2005 Ubiquitinated proliferating cell nuclear antigen activates translesion DNA polymerases eta and REV1. Proceedings of the National Academy of Sciences of the United States of America 202 16344468
2006 REV1 protein interacts with PCNA: significance of the REV1 BRCT domain in vitro and in vivo. Molecular cell 180 16857592
2002 Cellular roles of DNA polymerase zeta and Rev1 protein. DNA repair 179 12509231
2006 Ubiquitin-binding motifs in REV1 protein are required for its role in the tolerance of DNA damage. Molecular and cellular biology 176 16982685
2006 Strand-biased defect in C/G transversions in hypermutating immunoglobulin genes in Rev1-deficient mice. The Journal of experimental medicine 171 16476771
2000 The function of the human homolog of Saccharomyces cerevisiae REV1 is required for mutagenesis induced by UV light. Proceedings of the National Academy of Sciences of the United States of America 160 10760286
1999 The human REV1 gene codes for a DNA template-dependent dCMP transferase. Nucleic acids research 158 10536157
2004 The relative roles in vivo of Saccharomyces cerevisiae Pol eta, Pol zeta, Rev1 protein and Pol32 in the bypass and mutation induction of an abasic site, T-T (6-4) photoadduct and T-T cis-syn cyclobutane dimer. Genetics 156 15520252
2003 Rev1 is essential for DNA damage tolerance and non-templated immunoglobulin gene mutation in a vertebrate cell line. The EMBO journal 155 12660171
2021 REV1-Polζ maintains the viability of homologous recombination-deficient cancer cells through mutagenic repair of PRIMPOL-dependent ssDNA gaps. Molecular cell 153 34508659
2006 The critical mutagenic translesion DNA polymerase Rev1 is highly expressed during G(2)/M phase rather than S phase. Proceedings of the National Academy of Sciences of the United States of America 149 16751278
2005 Association of amino acid substitution polymorphisms in DNA repair genes TP53, POLI, REV1 and LIG4 with lung cancer risk. International journal of cancer 137 15609317
2002 Yeast Rev1 protein is a G template-specific DNA polymerase. The Journal of biological chemistry 132 11850424
2005 Vertebrate DNA damage tolerance requires the C-terminus but not BRCT or transferase domains of REV1. Nucleic acids research 130 15741181
1989 The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis. Journal of bacteriology 127 2492497
2002 Response of human REV1 to different DNA damage: preferential dCMP insertion opposite the lesion. Nucleic acids research 121 11917024
2012 Regulation of Rev1 by the Fanconi anemia core complex. Nature structural & molecular biology 116 22266823
2015 Regulation of the Rev1-pol ζ complex during bypass of a DNA interstrand cross-link. The EMBO journal 112 26071591
2010 Translesion synthesis across abasic lesions by human B-family and Y-family DNA polymerases α, δ, η, ι, κ, and REV1. Journal of molecular biology 111 20888339
2009 Differential roles for DNA polymerases eta, zeta, and REV1 in lesion bypass of intrastrand versus interstrand DNA cross-links. Molecular and cellular biology 111 20028736
2014 Determinants of G quadruplex-induced epigenetic instability in REV1-deficient cells. The EMBO journal 107 25190518
2010 Crystal structure of human REV7 in complex with a human REV3 fragment and structural implication of the interaction between DNA polymerase zeta and REV1. The Journal of biological chemistry 107 20164194
2006 Complex formation with Rev1 enhances the proficiency of Saccharomyces cerevisiae DNA polymerase zeta for mismatch extension and for extension opposite from DNA lesions. Molecular and cellular biology 107 17030609
2012 Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) ζ, and Pol κ. The Journal of biological chemistry 106 22859295
2011 REV1 and polymerase ζ facilitate homologous recombination repair. Nucleic acids research 105 21926160
2012 MiR-96 downregulates REV1 and RAD51 to promote cellular sensitivity to cisplatin and PARP inhibition. Cancer research 103 22761336
2020 Inhibition of the translesion synthesis polymerase REV1 exploits replication gaps as a cancer vulnerability. Science advances 100 32577513
2007 A ubiquitin-binding motif in the translesion DNA polymerase Rev1 mediates its essential functional interaction with ubiquitinated proliferating cell nuclear antigen in response to DNA damage. The Journal of biological chemistry 92 17517887
2012 Structural basis of recruitment of DNA polymerase ζ by interaction between REV1 and REV7 proteins. The Journal of biological chemistry 89 22859296
2004 Efficient and error-free replication past a minor-groove N2-guanine adduct by the sequential action of yeast Rev1 and DNA polymerase zeta. Molecular and cellular biology 87 15282292
2013 DNA polymerases ζ and Rev1 mediate error-prone bypass of non-B DNA structures. Nucleic acids research 86 24049079
2009 Identification of a novel REV1-interacting motif necessary for DNA polymerase kappa function. Genes to cells : devoted to molecular & cellular mechanisms 86 19170759
2009 Separate domains of Rev1 mediate two modes of DNA damage bypass in mammalian cells. Molecular and cellular biology 83 19332561
2006 REV3 and REV1 play major roles in recombination-independent repair of DNA interstrand cross-links mediated by monoubiquitinated proliferating cell nuclear antigen (PCNA). The Journal of biological chemistry 79 16571727
2008 The Fanconi anemia core complex is required for efficient point mutagenesis and Rev1 foci assembly. DNA repair 76 18448394
2005 Complex formation of yeast Rev1 and Rev7 proteins: a novel role for the polymerase-associated domain. Molecular and cellular biology 75 16227619
2012 Multifaceted recognition of vertebrate Rev1 by translesion polymerases ζ and κ. The Journal of biological chemistry 74 22700975
2005 The BRCT domain of mammalian Rev1 is involved in regulating DNA translesion synthesis. Nucleic acids research 71 15653636
2012 NMR structure and dynamics of the C-terminal domain from human Rev1 and its complex with Rev1 interacting region of DNA polymerase η. Biochemistry 70 22691049
2013 Human Rev1 polymerase disrupts G-quadruplex DNA. Nucleic acids research 67 24366879
2009 Structure of the human Rev1-DNA-dNTP ternary complex. Journal of molecular biology 67 19464298
2006 ATR homolog Mec1 controls association of DNA polymerase zeta-Rev1 complex with regions near a double-strand break. Current biology : CB 67 16546083
2016 Involvement of budding yeast Rad5 in translesion DNA synthesis through physical interaction with Rev1. Nucleic acids research 66 27001510
2010 Unconventional ubiquitin recognition by the ubiquitin-binding motif within the Y family DNA polymerases iota and Rev1. Molecular cell 65 20159559
2003 Structure and enzymatic properties of a stable complex of the human REV1 and REV7 proteins. The Journal of biological chemistry 60 12529368
2009 Oncogene homologue Sch9 promotes age-dependent mutations by a superoxide and Rev1/Polzeta-dependent mechanism. The Journal of cell biology 57 19687253
2008 Kinetic analysis of translesion synthesis opposite bulky N2- and O6-alkylguanine DNA adducts by human DNA polymerase REV1. The Journal of biological chemistry 56 18591245
2016 Interaction between the Rev1 C-Terminal Domain and the PolD3 Subunit of Polζ Suggests a Mechanism of Polymerase Exchange upon Rev1/Polζ-Dependent Translesion Synthesis. Biochemistry 55 26982350
2008 Protein-template-directed synthesis across an acrolein-derived DNA adduct by yeast Rev1 DNA polymerase. Structure (London, England : 1993) 53 18275815
2001 Deoxycytidyl transferase activity of the human REV1 protein is closely associated with the conserved polymerase domain. The Journal of biological chemistry 53 11278384
2009 Yeast Rev1 protein promotes complex formation of DNA polymerase zeta with Pol32 subunit of DNA polymerase delta. Proceedings of the National Academy of Sciences of the United States of America 52 19487673
2006 Yeast Rev1 is cell cycle regulated, phosphorylated in response to DNA damage and its binding to chromosomes is dependent upon MEC1. DNA repair 52 17035102
2018 Rev7 dimerization is important for assembly and function of the Rev1/Polζ translesion synthesis complex. Proceedings of the National Academy of Sciences of the United States of America 51 30111544
2006 Novel role for the C terminus of Saccharomyces cerevisiae Rev1 in mediating protein-protein interactions. Molecular and cellular biology 49 16923957
2009 Interaction with DNA polymerase eta is required for nuclear accumulation of REV1 and suppression of spontaneous mutations in human cells. DNA repair 48 19157994
2006 Human REV1 modulates the cytotoxicity and mutagenicity of cisplatin in human ovarian carcinoma cells. Molecular pharmacology 48 16495473
2017 Identification of Small Molecule Translesion Synthesis Inhibitors That Target the Rev1-CT/RIR Protein-Protein Interaction. ACS chemical biology 47 28541665
2006 A human DNA polymerase eta complex containing Rad18, Rad6 and Rev1; proteomic analysis and targeting of the complex to the chromatin-bound fraction of cells undergoing replication fork arrest. Genes to cells : devoted to molecular & cellular mechanisms 47 16824193
2013 NMR mapping of PCNA interaction with translesion synthesis DNA polymerase Rev1 mediated by Rev1-BRCT domain. Journal of molecular biology 45 23747975
2010 The DNA polymerase activity of Saccharomyces cerevisiae Rev1 is biologically significant. Genetics 45 20980236
2007 Complex formation of yeast Rev1 with DNA polymerase eta. Molecular and cellular biology 43 17875922
2020 REV1 inhibitor JH-RE-06 enhances tumor cell response to chemotherapy by triggering senescence hallmarks. Proceedings of the National Academy of Sciences of the United States of America 42 33168727
2016 The Proliferating Cell Nuclear Antigen (PCNA)-interacting Protein (PIP) Motif of DNA Polymerase η Mediates Its Interaction with the C-terminal Domain of Rev1. The Journal of biological chemistry 42 26903512
2015 FANCD2 and REV1 cooperate in the protection of nascent DNA strands in response to replication stress. Nucleic acids research 42 26187992
2012 REV1 and DNA polymerase zeta in DNA interstrand crosslink repair. Environmental and molecular mutagenesis 42 23065650
2011 The dCMP transferase activity of yeast Rev1 is biologically relevant during the bypass of endogenously generated AP sites. DNA repair 42 22024240
2008 Role of Dot1 in the response to alkylating DNA damage in Saccharomyces cerevisiae: regulation of DNA damage tolerance by the error-prone polymerases Polzeta/Rev1. Genetics 42 18562671
2012 The vital role of polymerase ζ and REV1 in mutagenic, but not correct, DNA synthesis across benzo[a]pyrene-dG and recruitment of polymerase ζ by REV1 to replication-stalled site. The Journal of biological chemistry 41 22303021
2005 Roles of the polymerase and BRCT domains of Rev1 protein in translesion DNA synthesis in yeast in vivo. Mutation research 41 15896814
2012 Rev1 recruits ung to switch regions and enhances du glycosylation for immunoglobulin class switch DNA recombination. Cell reports 39 23140944
2009 REV1 is implicated in the development of carcinogen-induced lung cancer. Molecular cancer research : MCR 39 19176310
2009 A critical role for REV1 in regulating the induction of C:G transitions and A:T mutations during Ig gene hypermutation. Journal of immunology (Baltimore, Md. : 1950) 38 19587019
2008 Novel conserved motifs in Rev1 C-terminus are required for mutagenic DNA damage tolerance. DNA repair 38 18603483
2011 The Rev1 translesion synthesis polymerase has multiple distinct DNA binding modes. DNA repair 37 21752727
2011 The non-canonical protein binding site at the monomer-monomer interface of yeast proliferating cell nuclear antigen (PCNA) regulates the Rev1-PCNA interaction and Polζ/Rev1-dependent translesion DNA synthesis. The Journal of biological chemistry 37 21799021
2008 Comparative analysis of in vivo interactions between Rev1 protein and other Y-family DNA polymerases in animals and yeasts. DNA repair 37 18242152
2001 Eukaryotic mutagenesis and translesion replication dependent on DNA polymerase zeta and Rev1 protein. Biochemical Society transactions 37 11356151
2015 Starvation promotes REV1 SUMOylation and p53-dependent sensitization of melanoma and breast cancer cells. Cancer research 34 25614517
2015 Rev1 promotes replication through UV lesions in conjunction with DNA polymerases η, ι, and κ but not DNA polymerase ζ. Genes & development 34 26680302
2006 Poleta, Polzeta and Rev1 together are required for G to T transversion mutations induced by the (+)- and (-)-trans-anti-BPDE-N2-dG DNA adducts in yeast cells. Nucleic acids research 33 16415180
2003 Ribozyme-mediated REV1 inhibition reduces the frequency of UV-induced mutations in the human HPRT gene. Nucleic acids research 33 12930947
2012 A non-catalytic function of Rev1 in translesion DNA synthesis and mutagenesis is mediated by its stable interaction with Rad5. DNA repair 32 23142547
2019 The Rev1-Polζ translesion synthesis mutasome: Structure, interactions and inhibition. The Enzymes 31 31627876
2010 DNA synthesis across an abasic lesion by yeast REV1 DNA polymerase. Journal of molecular biology 31 21167175
2002 Biochemical properties of the human REV1 protein. FEBS letters 30 12044876
2012 REV7 is required for anaphase-promoting complex-dependent ubiquitination and degradation of translesion DNA polymerase REV1. Cell cycle (Georgetown, Tex.) 28 23287467
2006 Role of single-stranded DNA in targeting REV1 to primer termini. The Journal of biological chemistry 28 16803901
2014 Mutational analysis of the C8-guanine adduct of the environmental carcinogen 3-nitrobenzanthrone in human cells: critical roles of DNA polymerases η and κ and Rev1 in error-prone translesion synthesis. Biochemistry 27 25080294
2013 Rev1 is essential in generating G to C transversions downstream of the Ung2 pathway but not the Msh2+Ung2 hybrid pathway. European journal of immunology 27 23857323
2004 Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography. The Biochemical journal 27 15175013
2004 REV1 accumulates in DNA damage-induced nuclear foci in human cells and is implicated in mutagenesis by benzo[a]pyrenediolepoxide. Nucleic acids research 27 15523096
2016 REV1 promotes PCNA monoubiquitylation through interacting with ubiquitylated RAD18. Journal of cell science 26 26795561
2013 DNA-damage tolerance mediated by PCNA*Ub fusions in human cells is dependent on Rev1 but not Polη. Nucleic acids research 26 23761444
2011 Molecular chaperone Hsp90 regulates REV1-mediated mutagenesis. Molecular and cellular biology 24 21690293
2009 Roles of Rev1, Pol zeta, Pol32 and Pol eta in the bypass of chromosomal abasic sites in Saccharomyces cerevisiae. Mutagenesis 24 19901007

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