Affinage

RAB31

Ras-related protein Rab-31 · UniProt Q13636

Length
195 aa
Mass
21.7 kDa
Annotated
2026-06-10
52 papers in source corpus 22 papers cited in narrative 22 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAB31 is a Rab5-subfamily small GTPase that governs selective membrane trafficking between the trans-Golgi network (TGN), early endosomes, and multivesicular endosomes (PMID:19345684, PMID:35863437). Its nucleotide cycle is driven by VPS9-domain GEFs: Gapex-5/RME-6, which couples RAB31 activity to insulin-regulated Glut4 vesicle translocation in adipocytes (PMID:17189207), and RIN3, which specifically loads GTP onto RAB31 to mobilize CD-MPR out of the TGN (PMID:21586568). In its active form RAB31 directs TGN-to-endosome transport of mannose-6-phosphate receptors, recruiting the PI(4,5)P2 5-phosphatase OCRL-1 to TGN carriers and acting at clathrin/GGA1-coated domains; its depletion collapses the Golgi (PMID:19345684, PMID:19795375). RAB31 distinguishes itself from the related RAB22 through a membrane targeting domain built from its C-terminal hypervariable domain, interswitch loop, and N-terminal region, which enforces Golgi over endosomal localization (PMID:35863437). On the endosomal/MVE axis, EGFR-phosphorylated RAB31 engages flotillins in lipid raft microdomains to drive ESCRT-independent intraluminal vesicle formation while recruiting the RAB7-GAP TBC1D2B to block MVE-lysosome fusion, thereby routing cargo toward exosome secretion (PMID:32958903); consistently, RAB31 controls EGFR transit from early to late endosomes via an EEA1- and Gapex-5-dependent complex (PMID:24644286). Beyond constitutive trafficking, RAB31 supports FcγR-mediated phagocytosis by recruiting APPL2 at phagocytic cups during the phosphoinositide transition (PMID:25568335), sustains TGF-βRII endocytosis required for TGF-β/Smad signaling (PMID:35091093), and its expression is transcriptionally driven by RUNX1 in megakaryocytes, where it is needed for early-endosomal trafficking of VWF, EGFR, and M6PR (PMID:35839075). RAB31 transcription is also activated by an ERα/MUC1-C complex in ER+ breast cancer, forming an autoinductive loop in which RAB31 stabilizes MUC1-C against lysosomal degradation (PMID:22792175). The Shigella effector IpaH4.5 acts as a RAB31-specific RabGAP, inactivating it to subvert CD-MPR-dependent cathepsin trafficking and evade lysosomal killing (PMID:34296983).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2002 Medium

    Established the basic biochemistry of RAB31 as a guanine nucleotide-binding protein, defining it as a functional small GTPase with atypical catalytic properties.

    Evidence GST-fusion recombinant protein with radiolabeled GTPγS binding and GTPase assays plus Q64L mutagenesis

    PMID:11784320

    Open questions at the time
    • No effectors or cellular pathway assigned
    • Atypical retention of activity by Q64L mutant left unexplained
    • Single-lab in vitro characterization
  2. 2007 High

    Identified the first physiological GEF (Gapex-5/RME-6) and a cellular role, linking RAB31 activity to insulin-regulated Glut4 vesicle trafficking and revealing its TGN localization.

    Evidence Yeast two-hybrid, GEF activity, and KD/OE with glucose uptake readouts in adipocytes; specific antibody localization to TGN with DN-mutant VSVG transport assay

    PMID:17189207 PMID:17678623

    Open questions at the time
    • Direct cargo of RAB31 in Glut4 pathway not defined
    • Connection between TGN dynamics and Glut4 retention unresolved
  3. 2009 High

    Defined RAB31's core trafficking function: selective TGN-to-endosome transport of mannose-6-phosphate receptors and broader receptor sorting, with Golgi integrity dependent on RAB31.

    Evidence CA/DN mutants, siRNA, cargo trafficking and colocalization assays for CD-MPR; reciprocal binding and KD trafficking assays for EGFR/CI-M6PR in A431 cells

    PMID:19345684 PMID:19725050

    Open questions at the time
    • Cargo selectivity mechanism (why CD-MPR but not CD63/VSVG) unexplained
    • Effectors mediating carrier formation not yet identified
  4. 2010 High

    Identified OCRL-1 as a direct RAB31 effector recruited to TGN carrier-forming domains, linking RAB31 to phosphoinositide control at the TGN.

    Evidence Yeast two-hybrid, GST pulldown, co-IP, and siRNA depletion with colocalization in oligodendrocytes

    PMID:19795375

    Open questions at the time
    • Functional consequence of OCRL-1 phosphatase activity for carrier budding not directly tested
    • Whether OCRL-1 recruitment is GTP-dependent not resolved
  5. 2011 High

    Demonstrated GEF specificity by reconstitution, establishing RIN3 as a RAB31-selective GEF that mobilizes CD-MPR from the TGN.

    Evidence Cell-free and in-cell GEF assays, site-directed mutagenesis, colocalization, and CD-MPR trafficking assay

    PMID:21586568

    Open questions at the time
    • Physiological signal activating RIN3 toward RAB31 unknown
    • Relationship between RIN3 and Gapex-5 GEF inputs not reconciled
  6. 2012 Medium

    Placed RAB31 in a transcription-trafficking feedback loop in breast cancer, where ERα/MUC1-C activate RAB31 and RAB31 stabilizes MUC1-C against lysosomal degradation.

    Evidence ChIP, promoter-reporter, DN mutant (S20N), and lysosomal degradation assays in ER+ breast cancer cells

    PMID:22792175

    Open questions at the time
    • Mechanism by which RAB31 diverts MUC1-C from lysosomes not detailed
    • Generality beyond ER+ context untested
  7. 2014 High

    Extended RAB31 function to endosomal maturation, showing it routes ligand-bound EGFR from early to late endosomes via an EEA1/Gapex-5-containing complex.

    Evidence Co-IP, affinity pulldown, glycerol gradient sedimentation, KD/OE, and pulse-chase EGFR trafficking assays

    PMID:24644286

    Open questions at the time
    • Stoichiometry and assembly order of the RAB31-EEA1-Gapex5 complex unresolved
    • How RAB31 reconciles pro-degradation versus exosome-routing fates of EGFR not addressed
  8. 2015 High

    Revealed RAB31 as a coordinator of phagosome maturation, recruiting APPL2 at the phosphoinositide transition to support FcγR-mediated phagocytosis and PI3K/Akt signaling.

    Evidence Live-cell imaging with phosphoinositide reporters, siRNA, phagocytosis efficiency, and signaling pathway analysis in macrophages

    PMID:25568335

    Open questions at the time
    • Whether APPL2 recruitment is direct GTP-dependent binding not biochemically confirmed
    • GEF driving RAB31 activation at the phagocytic cup unidentified
  9. 2020 High

    Resolved a mechanism for ESCRT-independent exosome biogenesis, showing EGFR-phosphorylated RAB31 uses flotillins to form ILVs while recruiting TBC1D2B to inactivate RAB7 and block lysosomal fusion.

    Evidence Co-IP, domain mapping, DA/DN mutants, KD/OE with ILV formation, exosome secretion, and lysosome fusion assays

    PMID:32958903

    Open questions at the time
    • The EGFR phosphosite(s) on RAB31 and their regulatory effect not fully mapped
    • Balance between flotillin ILV pathway and canonical ESCRT routing unclear
  10. 2021 High

    Showed RAB31 is a target of bacterial subversion, with the Shigella RabGAP effector IpaH4.5 inactivating it to disrupt M6PR-dependent cathepsin delivery and evade lysosomal killing.

    Evidence In vitro GAP activity assay, co-IP, MPR trafficking microscopy, cathepsin B activity, and intracellular persistence assays

    PMID:34296983

    Open questions at the time
    • Endogenous host RabGAP for RAB31 still unknown
    • Whether IpaH4.5 also affects RAB31's exosomal/endosomal roles untested
  11. 2022 High

    Identified the structural determinant of RAB31 organelle targeting and a transcriptional driver, while expanding its physiological roles to megakaryocyte granule biogenesis, TGF-β signaling, and RAGE endocytosis.

    Evidence Domain-swap chimeras and Rabenosyn-5 KO localization; RUNX1 promoter/CRISPR KO with cargo tracking in iPSC-megakaryocytes; KD TGF-βRII endocytosis with CCl4 fibrosis model; GST-MS/co-IP with RAGE endocytosis and β-cell apoptosis assays

    PMID:35091093 PMID:35314532 PMID:35839075 PMID:35863437

    Open questions at the time
    • How the HVD/interswitch MTD physically discriminates Golgi from endosomal membranes mechanistically unresolved
    • Whether RAGE and TGF-βRII handling share the EGFR endosomal machinery untested
  12. 2023 Medium

    Linked RAB31-driven exosome biogenesis to tumor progression, showing RAB31 controls exosome number/size and selective EV cargo loading that promotes metastasis and invasion.

    Evidence Nanoparticle tracking, electron microscopy, mass spectrometry, and in vivo exosome injection/metastasis models in gastric cancer and glioma EC systems

    PMID:37222416 PMID:37953466

    Open questions at the time
    • Mechanism of selective cargo (PSMA1, MYO1C) sorting into RAB31-dependent EVs undefined
    • Glioma EV study rests on single-KD evidence without reconstitution

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RAB31 mechanistically partitions a single cargo (e.g. EGFR) between degradative late-endosome routing, exosomal secretion, and recycling, and which GEF/effector combinations select each fate, remains unresolved.
  • No unified model reconciling pro-degradation versus exosome-secretory RAB31 functions
  • Spatiotemporal regulation of competing effectors (OCRL-1, flotillin, TBC1D2B, APPL2, EEA1) not integrated
  • Structural basis of effector selection beyond the membrane targeting domain unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Localization
GO:0005768 endosome 4 GO:0005794 Golgi apparatus 4 GO:0005886 plasma membrane 2 GO:0031410 cytoplasmic vesicle 2
Pathway
R-HSA-5653656 Vesicle-mediated transport 4 R-HSA-162582 Signal Transduction 3 R-HSA-168256 Immune System 2 R-HSA-1852241 Organelle biogenesis and maintenance 2 R-HSA-9609507 Protein localization 2
Partners
APPL2EEA1EGFRFLOT1GAPEX5OCRLRIN3TBC1D2B

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2020 Active RAB31, phosphorylated by EGFR, engages flotillin proteins in lipid raft microdomains to drive EGFR entry into MVEs to form intraluminal vesicles (ILVs) independently of the ESCRT machinery. RAB31 interacts with the SPFH domain of flotillin and drives ILV formation via the flotillin domain. Simultaneously, RAB31 recruits GTPase-activating protein TBC1D2B to inactivate RAB7, thereby preventing MVE-lysosome fusion and enabling ILV secretion as exosomes. Co-immunoprecipitation, functional rescue, dominant-active/inactive mutants, knockdown/overexpression with phenotypic readouts (ILV formation, exosome secretion, lysosome fusion assays) Cell research High 32958903
2007 Gapex-5/RME-6 contains a VPS9 domain that acts as a guanine nucleotide exchange factor (GEF) for RAB31. In adipocytes, overexpression of RAB31 blocks insulin-stimulated Glut4 translocation, while knockdown potentiates it. Insulin recruits the CIP4/Gapex-5 complex to the plasma membrane, reducing RAB31 activity and permitting Glut4 vesicles to translocate to the cell surface. Yeast two-hybrid, overexpression/knockdown in adipocytes, glucose uptake assays, subcellular localization Cell metabolism High 17189207
2011 RIN3 specifically acts as a guanine nucleotide exchange factor (GEF) for RAB31 (but not RAB21), stimulating GTP-bound RAB31 formation in cell-free and in-cell GEF activity assays. Serine-to-alanine substitutions in the sequence between SH2 and RIN family homology domain of RIN3 specifically abolished its GEF action on RAB31 but not RAB5. RIN3 colocalizes with RAB31 in enlarged vesicles and tubular structures in HeLa cells. RIN3 partially translocates CD-MPR from the TGN to peripheral vesicles in a RAB31-GEF-dependent manner. Cell-free and in-cell GEF activity assays, site-directed mutagenesis, colocalization by fluorescence microscopy, CD-MPR trafficking assay The Journal of biological chemistry High 21586568
2002 Recombinant RAB31 expressed as a GST-fusion protein binds [35S]GTPγS in a Mg2+-dependent manner (optimal at 5 µM free Mg2+, inhibited at higher concentrations). RAB31 displays low steady-state GTPase activity. The Q64L GTPase-dead mutation does not abolish GTPase activity of RAB31 (unlike most small GTPases). GST-fusion protein expression, radiolabeled nucleotide binding assay, GTPase activity assay, site-directed mutagenesis European journal of biochemistry Medium 11784320
2009 RAB31 is required for transport of mannose-6-phosphate receptors (specifically cation-dependent CD-MPR, but not CD63 or VSVG) from the TGN to endosomes. CD-MPR and RAB31 colocalize in TGN carriers containing clathrin and GGA1 coats. Constitutively active RAB31 redistributes CD-MPR from TGN to endosomes; dominant-negative RAB31 causes the reverse. siRNA depletion of RAB31 causes collapse of the Golgi apparatus. Dominant-active/inactive RAB31 mutants, siRNA knockdown, immunofluorescence colocalization, cargo trafficking assays Experimental cell research High 19345684
2010 RAB31 interacts with OCRL-1 (a PI(4,5)P2 5-phosphatase) as shown by yeast two-hybrid, GST-RAB31 pulldown, and co-immunoprecipitation from oligodendrocyte lysates. RAB31 and OCRL-1 colocalize in TGN, post-TGN carriers, and endosomes. siRNA depletion of RAB31 causes TGN collapse and markedly decreases OCRL-1 levels in the TGN and endosomes, indicating RAB31 recruits OCRL-1 to TGN domains where carriers form. Yeast two-hybrid, GST pulldown, co-immunoprecipitation, siRNA knockdown, immunofluorescence colocalization Journal of neuroscience research High 19795375
2007 Endogenous RAB22B/RAB31 is largely localized to the TGN in HeLa cells. Expression of dominant-negative GDP-bound RAB22B (but not wild-type) specifically disrupts TGN46 localization and inhibits anterograde exit of VSVG from the TGN, implicating RAB31 in anterograde TGN membrane dynamics. Specific antibody, overexpression of dominant-negative mutant, immunofluorescence, VSVG transport assay Biochemical and biophysical research communications Medium 17678623
2009 RAB22B/RAB31 is expressed specifically in nestin/RC2-positive radial glia of the embryonic mouse brain and in GFAP-positive astrocytes of the adult brain. Silencing RAB22B in A431 cells causes abnormal trafficking of EGFR, Texas-red-EGF, and cation-independent M6PR. RAB22B associates with EGFR in a GTP-dependent manner as shown by affinity pulldown and co-immunoprecipitation. Specific antibody, immunofluorescence, siRNA knockdown, affinity pulldown, co-immunoprecipitation, receptor trafficking assays Journal of cellular physiology Medium 19725050
2014 RAB31 regulates trafficking of ligand-bound EGFR from early to late endosomes. Loss of RAB31 inhibits, and overexpression enhances, EGFR trafficking to late endosomes. RAB31 interacts with EGFR (by co-IP and affinity pulldown) and is recruited into a high-molecular-weight complex with EEA1 after EGF stimulation. Loss of EEA1 reduces RAB31-EGFR interaction and abolishes the effect of RAB31 on EGFR trafficking. Loss of GAPex5 also reduces RAB31-EGFR interaction. Co-immunoprecipitation, affinity pulldown, glycerol gradient sedimentation, siRNA knockdown, overexpression, pulse-chase EGFR trafficking assay The Journal of biological chemistry High 24644286
2015 RAB31 is recruited to early-stage phagocytic cups in macrophages at the PI(4,5)P2-to-PI(3,4,5)P3 phosphoinositide transition and persists on PI(3)P-enriched phagosomes. RAB31-GTP recruits the signaling adaptor APPL2 at phagocytic cups. siRNA depletion of either RAB31 or APPL2 reduces FcγR-mediated phagocytosis, delays transition to PI(3,4,5)P3 and phagocytic cup closure, reduces PI3K/Akt signaling, and enhances p38 signaling. Live-cell imaging, siRNA knockdown, phosphoinositide reporters, FcγR-mediated phagocytosis assay, signaling pathway analysis Molecular biology of the cell High 25568335
2012 MUC1-C forms a complex with ERα on the RAB31 promoter and activates RAB31 gene transcription in an estrogen-dependent manner in ER+ breast cancer cells. In turn, RAB31 attenuates lysosomal degradation of MUC1-C, creating an autoinductive loop. Expression of inactive RAB31(S20N) mutant in nonmalignant breast epithelial cells confirmed RAB31 regulates MUC1-C expression. Chromatin immunoprecipitation, promoter-reporter assay, dominant-negative mutant, lysosomal degradation assay, mammosphere formation assay PloS one Medium 22792175
2022 RAB31 is regulated by the transcription factor RUNX1, which binds the RAB31 promoter in megakaryocytic cells. Downregulation of RUNX1 or RAB31 (by siRNA or CRISPR/Cas9) causes striking enlargement of early endosomes (EEs), partially reversed by RAB31 reconstitution. RAB31 deficiency impairs trafficking of VWF (to α-granules), EGFR, and mannose-6-phosphate receptor at the level of EEs in megakaryocytic cells. Promoter-reporter assay, siRNA, CRISPR/Cas9 knockout, RAB31 reconstitution, immunofluorescence for EE markers, iPSC-derived megakaryocyte model Blood advances High 35839075
2022 RAB31 knockdown inhibits TGF-β receptor II complex endocytosis in hepatic stellate cells, impairing TGF-β/Smad signaling and preventing hepatic stellate cell activation. RAB31 is required as a prerequisite for TGF-βRII endocytosis-driven TGF-β signaling. Lentiviral knockdown, TGF-βRII endocytosis assay, Smad signaling analysis, mouse CCl4 fibrosis model The international journal of biochemistry & cell biology Medium 35091093
2021 The Shigella effector IpaH4.5 harbors TBC-like dual-finger motifs and exhibits potent RabGAP activity specifically toward RAB31, inactivating it. This disrupts CD-MPR transport from the Golgi to endosomes and attenuates lysosomal cathepsin B activity, allowing Shigella to escape lysosomal degradation. Intracellular persistence of S. flexneri requires IpaH4.5 TBC-like GAP activity. GAP activity assay (in vitro), co-immunoprecipitation, confocal microscopy (MPR trafficking), Magic Red-RR cathepsin B activity assay, intracellular persistence assay Journal of medical microbiology High 34296983
2022 The C-terminal hypervariable domain (HVD) of RAB31, together with the interswitch loop and N-terminal domain, constitutes a membrane targeting domain (MTD) that dictates Golgi (rather than early endosomal) localization of RAB31. Replacing the RAB31 HVD with the RAB22 HVD shifts RAB31 to early endosomes. RAB31 Golgi localization is further influenced by differential interaction with the early endosomal effector Rabenosyn-5, which stabilizes RAB22 at endosomes but does not stabilize RAB31 there. Domain-swap chimera mutants, live fluorescence microscopy, Rabenosyn-5 knockout cells, co-localization analysis The Journal of biological chemistry Medium 35863437
2022 RAB31 interacts with RAGE (receptor for advanced glycation end products) intracellular domain, identified by GST pulldown combined with mass spectrometry and confirmed by co-immunoprecipitation and immunostaining. This interaction is enhanced by glycation-serum stimulation and is associated with membrane redistribution of RAB31. RAB31 promotes RAGE endocytosis and inhibits AGE-induced β-cell apoptosis through the pAKT/BCL2 pathway. GST pulldown combined with mass spectrometry, co-immunoprecipitation, immunostaining, RAGE endocytosis assay, apoptosis assay Endocrine journal Medium 35314532
2018 NCSTN mutations reduce miR-30a-3p levels, which negatively regulates RAB31 expression. Enhanced RAB31 levels accelerate degradation of activated EGFR, leading to abnormal keratinocyte differentiation. The miR-30a-3p/RAB31/EGFR signaling axis was demonstrated in familial acne inversa patient samples and NcstnΔKC mice. miRNA microarray, Ncstn keratinocyte-specific knockout mice, luciferase reporter assay (miRNA target validation), EGFR degradation assay, keratinocyte differentiation assay The Journal of investigative dermatology Medium 30120935
2014 RAB31 interacts with GLI1 as confirmed by co-immunoprecipitation and immunofluorescence in gastric cancer cells. RAB31 silencing suppresses cell viability, promotes cell cycle arrest, enhances apoptosis, and affects cell cycle/apoptotic proteins, effects mediated through GLI1. Co-immunoprecipitation, immunofluorescence, siRNA knockdown, cell viability/apoptosis assays, luciferase reporter assay (miRNA target validation) Frontiers in oncology Low 30534536
2014 RAB31 is expressed in neural progenitor cells (NPCs). Silencing RAB31 hinders, while overexpression enhances, differentiation of NPCs to astrocytes, establishing a role for RAB31 in NPC fate determination. Primary NPC culture, siRNA knockdown, overexpression, differentiation assay with GFAP/nestin markers FEBS letters Low 24999186
2022 RAB31 interacts with MAPK6, and RAB31 knockdown reduces MAPK6 protein levels by promoting its degradation. MAPK6 overexpression restores the decreased migration potential caused by RAB31 knockdown, placing RAB31 upstream of MAPK6 in cervical cancer cell migration. Co-immunoprecipitation, MAPK6 degradation assay, overexpression rescue, migration assay, in vivo xenograft International journal of biological sciences Medium 34975321
2023 RAB31 overexpression in gastric cancer cells increases exosome secretion (number), while RAB31 depletion reduces both the number and size of secreted exosomes. Injection of exosomes derived from RAB31-overexpressing cells promotes pulmonary metastasis in vivo. PSMA1 was identified as an exosomal protein overexpressed in concert with RAB31. Exosome nanoparticle tracking analysis, electron microscopy, in vivo exosome injection/metastasis model, protein mass spectrometry Cancer medicine Medium 37222416
2023 RAB31 in glioma-derived endothelial cells drives enrichment of MYO1C into extracellular vesicles (EVs). RAB31 knockdown reduces MYO1C enrichment in secretory EVs and attenuates promotion of glioma cell invasion by GhEC-EVs. This EV export mechanism is also dependent on RAB27B and FAS. siRNA knockdown, EV isolation, Western blot for MYO1C in EVs, invasion assay FEBS open bio Low 37953466

Source papers

Stage 0 corpus · 52 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2020 RAB31 marks and controls an ESCRT-independent exosome pathway. Cell research 395 32958903
2007 Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes. Cell metabolism 93 17189207
2014 The role of the small GTPase Rab31 in cancer. Journal of cellular and molecular medicine 58 25472813
2015 Rab31 promoted hepatocellular carcinoma (HCC) progression via inhibition of cell apoptosis induced by PI3K/AKT/Bcl-2/BAX pathway. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 57 26044564
2011 Characterization of RIN3 as a guanine nucleotide exchange factor for the Rab5 subfamily GTPase Rab31. The Journal of biological chemistry 54 21586568
2002 Molecular cloning, bacterial expression and properties of Rab31 and Rab32. European journal of biochemistry 46 11784320
2015 The Critical Role of Rab31 in Cell Proliferation and Apoptosis in Cancer Progression. Molecular neurobiology 45 26245486
2015 miR-184 and miR-150 promote renal glomerular mesangial cell aging by targeting Rab1a and Rab31. Experimental cell research 41 26165933
2018 Nicastrin/miR-30a-3p/RAB31 Axis Regulates Keratinocyte Differentiation by Impairing EGFR Signaling in Familial Acne Inversa. The Journal of investigative dermatology 38 30120935
2009 Transport of mannose-6-phosphate receptors from the trans-Golgi network to endosomes requires Rab31. Experimental cell research 38 19345684
2018 RAB31 Targeted by MiR-30c-2-3p Regulates the GLI1 Signaling Pathway, Affecting Gastric Cancer Cell Proliferation and Apoptosis. Frontiers in oncology 37 30534536
2015 Rab31 and APPL2 enhance FcγR-mediated phagocytosis through PI3K/Akt signaling in macrophages. Molecular biology of the cell 37 25568335
2012 Cooperative interaction between the MUC1-C oncoprotein and the Rab31 GTPase in estrogen receptor-positive breast cancer cells. PloS one 34 22792175
2023 Rab31 promotes metastasis and cisplatin resistance in stomach adenocarcinoma through Twist1-mediated EMT. Cell death & disease 33 36781842
2014 Engagement of the small GTPase Rab31 protein and its effector, early endosome antigen 1, is important for trafficking of the ligand-bound epidermal growth factor receptor from the early to the late endosome. The Journal of biological chemistry 33 24644286
2007 Rab22B's role in trans-Golgi network membrane dynamics. Biochemical and biophysical research communications 31 17678623
2020 Increased RAB31 Expression in Cancer-Associated Fibroblasts Promotes Colon Cancer Progression Through HGF-MET Signaling. Frontiers in oncology 29 33072555
2022 Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation. International journal of biological sciences 25 34975321
2010 Interaction of Rab31 and OCRL-1 in oligodendrocytes: its role in transport of mannose 6-phosphate receptors. Journal of neuroscience research 24 19795375
2009 Rab22B is expressed in the CNS astroglia lineage and plays a role in epidermal growth factor receptor trafficking in A431 cells. Journal of cellular physiology 23 19725050
2022 MiR-378a-3p acts as a tumor suppressor in gastric cancer via directly targeting RAB31 and inhibiting the Hedgehog pathway proteins GLI1/2. Cancer biology & medicine 16 36245214
2020 FGF2 Affects Parkinson's Disease-Associated Molecular Networks Through Exosomal Rab8b/Rab31. Frontiers in genetics 16 33101391
2023 lncRNA MAGI2-AS3 suppresses castration-resistant prostate cancer proliferation and migration via the miR-106a-5p/RAB31 axis. Genomics 15 36889366
2020 miR-425-5p, a SOX2 target, regulates the expression of FOXJ3 and RAB31 and promotes the survival of GSCs. Archives of clinical and biomedical research 14 32905473
2019 Oncogenic Effect of the Novel Fusion Gene VAPA-Rab31 in Lung Adenocarcinoma. International journal of molecular sciences 13 31083279
2023 Lysosome-related exosome secretion mediated by miR-26b / Rab31 pathway was associated with the proliferation and migration of MCF-7 cells treated with BPA. Ecotoxicology and environmental safety 11 36701876
2018 Inhibitory effect of microRNA-455-5p on biological functions of esophageal squamous cell carcinoma Eca109 cells via Rab31. Experimental and therapeutic medicine 11 30542452
2023 Overexpression of RAB31 in gastric cancer is associated with released exosomes and increased tumor cell invasion and metastasis. Cancer medicine 10 37222416
2022 Defective RAB31-mediated megakaryocytic early endosomal trafficking of VWF, EGFR, and M6PR in RUNX1 deficiency. Blood advances 9 35839075
2020 GANT61 plays antitumor effects by inducing oxidative stress through the miRNA-1286/RAB31 axis in osteosarcoma. Cell biology international 9 32936498
2022 LncRNA HOXA10-AS Activated by E2F1 Facilitates Proliferation and Migration of Nasopharyngeal Carcinoma Cells Through Sponging miR-582-3p to Upregulate RAB31. American journal of rhinology & allergy 8 35072529
2022 Rab31 promotes activation of hepatic stellate cells by accelerating TGF-β receptor II complex endocytosis. The international journal of biochemistry & cell biology 8 35091093
2020 RAB31 is targeted by miR-26b and serves a role in the promotion of osteosarcoma. Oncology letters 8 32973957
2022 LRG-1 promotes fat graft survival through the RAB31-mediated inhibition of hypoxia-induced apoptosis. Journal of cellular and molecular medicine 7 35322540
2022 Rab31, a receptor of advanced glycation end products (RAGE) interacting protein, inhibits AGE induced pancreatic β-cell apoptosis through the pAKT/BCL2 pathway. Endocrine journal 6 35314532
2021 Shigella escapes lysosomal degradation through inactivation of Rab31 by IpaH4.5. Journal of medical microbiology 6 34296983
2021 Rab31-dependent regulation of transforming growth factor ß expression in breast cancer cells. Molecular medicine (Cambridge, Mass.) 6 34906074
2018 Effect of RAB31 silencing on osteosarcoma cell proliferation and migration through the Hedgehog signaling pathway. Journal of bone and mineral metabolism 6 30470957
2014 Rab31 is expressed in neural progenitor cells and plays a role in their differentiation. FEBS letters 6 24999186
2022 A novel membrane targeting domain mediates the endosomal or Golgi localization specificity of small GTPases Rab22 and Rab31. The Journal of biological chemistry 5 35863437
2024 RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer. Journal of extracellular biology 4 38939899
2025 Non-coding RNA RMRP governs RAB31-dependent MMP secretion, enhancing ovarian cancer invasion. Biochimica et biophysica acta. Molecular basis of disease 3 40057205
2023 RAB31 in glioma-derived endothelial cells promotes glioma cell invasion via extracellular vesicle-mediated enrichment of MYO1C. FEBS open bio 3 37953466
2022 Association between Rab31/rs9965664 polymorphism and immunoglobulin therapy resistance in patients with Kawasaki disease. Frontiers in cardiovascular medicine 3 36329997
2025 EGFR/STAT3 signaling mediates the upregulation of CD47 in HPV-positive cervical cancer by activating p65 and exosome transporter RAB31. Neoplasma 2 40353624
2025 The Cx43-Mediated Autophagy Mechanism Influences Triple-Negative Breast Cancer Through the Regulation of Rab31. Cancers 2 41463174
2018 Integrative functional genomics identifies regulatory genetic variant modulating RAB31 expression and altering susceptibility to breast cancer. Molecular carcinogenesis 2 30182384
2025 RETRACTION: GANT61 Plays Antitumor Effects by Inducing Oxidative Stress through the miRNA-1286/RAB31 Axis in Osteosarcoma. Cell biology international 1 39743787
2026 RAB31 orchestrates CXCL2-CXCR4-mediated neutrophil recruitment and proangiogenic niche formation in colorectal cancer. Journal of translational medicine 0 41749320
2025 Retraction: RAB31 targeted by MiR-30c-2-3p regulates the GLI1 signaling pathway, affecting gastric cancer cell proliferation and apoptosis. Frontiers in oncology 0 41114346
2021 Erratum: miR-425-5p, a SOX2 target, regulates the expression of FOXJ3 and RAB31 and promotes the survival of GSCs. Archives of clinical and biomedical research 0 34888488
2020 Correction to: Effect of RAB31 silencing on osteosarcoma cell proliferation and migration through the Hedgehog signaling pathway. Journal of bone and mineral metabolism 0 32514735

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