Affinage

OTUB1

Ubiquitin thioesterase OTUB1 · UniProt Q96FW1

Length
271 aa
Mass
31.3 kDa
Annotated
2026-06-10
100 papers in source corpus 47 papers cited in narrative 47 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

OTUB1 is an OTU-family deubiquitinase that controls the stability of a broad set of regulatory proteins through two mechanistically distinct activities: canonical cleavage of Lys48-linked polyubiquitin chains, and a catalysis-independent suppression of ubiquitin transfer by E2 conjugating enzymes (PMID:20725033, PMID:22325355, PMID:23955022). The non-canonical mode was defined at DNA double-strand breaks, where OTUB1 binds and inhibits the E2 UBC13 (UBE2N) to suppress RNF168-dependent K63-linked ubiquitination, blocking 53BP1 focus formation independently of catalytic activity, and extends to UBE2D and UBE2E E2 subfamilies (PMID:20725033). Crystallographic and biochemical work established the molecular basis: free ubiquitin binds a distal allosteric site to fold an N-terminal ubiquitin-binding helix, increasing OTUB1 affinity for the donor ubiquitin of the charged E2~Ub thioester, occluding the RING-E3 binding surface on UBC13 and blocking acceptor attack, while the canonical K48 distal ubiquitin site is co-opted to engage the thioester (PMID:22367539, PMID:22325355, PMID:22679021); reciprocally, E2 enzymes stimulate OTUB1's intrinsic K48 isopeptidase activity through the same helix-folding event, creating bidirectional cross-regulation governed by the ratio of charged to free ubiquitin (PMID:23955022). Through these activities OTUB1 stabilizes numerous substrates by either editing K48 chains or shielding them from E2-mediated ubiquitination, including phospho-SMAD2/3 in TGFβ signaling (PMID:24071738), c-IAP1 to sustain NF-κB/MAPK signaling and prevent necroptosis (PMID:23524849, PMID:33712742), FOXM1 (PMID:26148240), RAS (PMID:26881969), MYC (PMID:35296795), YAP (PMID:36271031), PD-L1 (PMID:33328570), and the cystine antiporter SLC7A11 and GPX4 to restrain ferroptosis (PMID:30709928, PMID:36369321). p53 pathway control is exerted indirectly: OTUB1 suppresses MDM2-mediated ubiquitination of p53 and MDMX via non-canonical E2 (UbcH5) inhibition, an activity that itself requires OTUB1 monoubiquitination at Lys59/Lys109 (PMID:24403071, PMID:28035068). OTUB1 also tunes innate and adaptive immunity, deubiquitinating TRAF3/TRAF6 to dampen interferon and TLR responses (PMID:19996094, PMID:24763515, PMID:32024978), setting an IL-15/AKT activation threshold in CD8+ T and NK cells (PMID:31182807), and stabilizing p100 and SOCS1 to constrain NF-κB and JAK/STAT signaling, with conditional knockouts producing autoimmunity and severe experimental autoimmune encephalomyelitis (PMID:31086255, PMID:30944096). OTUB1 activity is itself extensively regulated by post-translational modification: CK2 phosphorylation at Ser16 drives nuclear accumulation and DNA-repair function (PMID:25872870), Src/SRMS phosphorylation at Tyr26 enables Raptor binding (PMID:35927303), SET7 methylation at Lys122 impairs UBC13 engagement (PMID:36822329), Cys91 persulfidation enhances E2 binding to stabilize SLC7A11 (PMID:37703360), and FIH-mediated asparagine hydroxylation/covalent complexation links OTUB1 to oxygen sensing (PMID:26752685, PMID:31299612). Otub1 is essential for development, as knockout mice are embryonic lethal (PMID:30282802).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 2009 Medium

    Established OTUB1 as a deubiquitinase acting on innate immune signaling adaptors, the first functional context placing it in interferon regulation.

    Evidence Co-IP and IFN-β reporter/antiviral assays with TRAF3 and TRAF6 in cells

    PMID:19996094

    Open questions at the time
    • Did not resolve catalytic vs. non-catalytic mechanism
    • Single lab without structural validation
  2. 2009 Medium

    Showed OTUB1 directly deubiquitinates a transcription factor (ERα), introducing the theme of substrate stabilization on chromatin.

    Evidence Mass spec of ERα complex, in vitro DUB assay, ChIP and reporter assays

    PMID:19383985

    Open questions at the time
    • Ubiquitin chain linkage specificity not defined
    • Single lab
  3. 2010 High

    Discovered the catalysis-independent mode of action—OTUB1 inhibits K63 chain synthesis by binding the E2 UBC13 rather than cleaving chains—redefining how a DUB can restrict ubiquitination.

    Evidence Co-IP, in vitro ubiquitination, catalytic-mutant analysis, and DSB repair readouts

    PMID:20725033

    Open questions at the time
    • Structural basis of E2 inhibition not yet resolved at this stage
    • Regulation of the switch between canonical and non-canonical modes unknown
  4. 2012 High

    Provided the structural mechanism: free ubiquitin allosterically activates OTUB1, folding an N-terminal helix that captures the E2~Ub thioester and occludes the RING-E3 site, explaining non-canonical E2 inhibition.

    Evidence X-ray crystallography of OTUB1–UBC13 and OTUB1–Ub aldehyde–UBC13~Ub conjugates, SPR, structure-guided mutagenesis (three independent studies)

    PMID:22325355 PMID:22367539 PMID:22679021

    Open questions at the time
    • In vivo regulation of allosteric ubiquitin pool not addressed
    • Which substrates depend on this mode in cells left open
  5. 2013 High

    Demonstrated bidirectional cross-regulation—E2 enzymes stimulate OTUB1 K48 cleavage via the same helix-folding event—integrating its two activities into one allosteric framework.

    Evidence Reconstituted DUB activity assays and crystallography of human and worm OTUB1/UBCH5B with mutagenesis

    PMID:23955022

    Open questions at the time
    • Cellular thresholds of charged/uncharged E2 not quantified
    • Substrate-specific consequences not mapped
  6. 2013 High

    Connected the non-canonical mechanism to physiological signaling outputs by showing OTUB1 sustains phospho-SMAD2/3 and stabilizes c-IAP1, controlling TGFβ-driven migration and TWEAK/NF-κB survival signaling.

    Evidence Signal-dependent Co-IP, catalytic mutants, proteasome-inhibitor rescue, zebrafish c-IAP knockdown, functional migration/death assays

    PMID:23524849 PMID:24071738

    Open questions at the time
    • Recruitment determinants to each signaling complex not fully defined
  7. 2014 High

    Revealed auto-regulation of OTUB1—its own monoubiquitination at Lys59/109 is required to suppress UbcH5 and stabilize p53—and that knockout is embryonically lethal, marking developmental essentiality.

    Evidence In vitro monoubiquitination/ubiquitination assays with lysine-free mutant, CHX chase, apoptosis assays; HSCARG-dependent TRAF3 recruitment; later KO mouse

    PMID:24403071 PMID:24763515

    Open questions at the time
    • How monoubiquitination remodels OTUB1 structurally not resolved
  8. 2016 Medium

    Extended substrate range to oncogenic RAS and identified oxygen-dependent FIH hydroxylation at Asn22 as a regulator, linking OTUB1 to metabolism and hypoxia.

    Evidence In vitro ubiquitination/imaging/xenografts for RAS; in vitro hydroxylation, N22A interactome and metabolic assays for FIH

    PMID:26752685 PMID:26881969

    Open questions at the time
    • Physiological oxygen tension dependence in vivo not established
    • FIH-OTUB1 stoichiometry unresolved at this stage
  9. 2018 High

    Showed OTUB1 stabilizes the E2 enzyme UBE2E1 itself and the mTOR inhibitor DEPTOR, positioning OTUB1 as a regulator of E2 abundance and mTORC1/autophagy, and confirmed embryonic lethality of Otub1 knockout.

    Evidence In vitro autoubiquitination assays, mouse and human KO cells, Asp88/Cys91 mutant dissection, mTORC1 and autophagy readouts

    PMID:29382726 PMID:30282802

    Open questions at the time
    • Non-canonical Asp88-dependent catalysis mechanism incompletely defined
  10. 2019 High

    Established in vivo immunoregulatory roles via conditional knockouts: OTUB1 sets an IL-15/AKT activation threshold and stabilizes p100, with deficiency causing enhanced anti-tumor immunity or lupus-like autoimmunity.

    Evidence Conditional Otub1 KO mice, membrane fractionation, AKT/NF-κB ubiquitination assays, immune functional and tumor-challenge models

    PMID:31086255 PMID:31182807

    Open questions at the time
    • Tissue-specific substrate selection mechanisms not fully defined
  11. 2020 High

    Broadened OTUB1 into cancer immune evasion and context-dependent E2 control: it stabilizes PD-L1 to suppress CD8+ cytotoxicity, yet in dendritic cells stabilizes UBC13 to promote NF-κB, and relocalizes to mitochondria to activate RIG-I.

    Evidence K48-specific DUB assays, OTUB1 KO/CRISPR cells, DC-specific KO mice, cell-free RIG-I reconstitution, immune functional and infection models

    PMID:32023470 PMID:32024978 PMID:33328570

    Open questions at the time
    • What dictates whether OTUB1 stabilizes vs. inhibits a given E2 in a given cell remains unresolved
  12. 2021 High

    Defined OTUB1 as a node in tissue protection and metabolism, stabilizing SOCS1, c-IAP1 (anti-necroptosis), MYC, YAP, and SLC7A11, linking it to JAK/STAT control, glycolysis, Hippo signaling, and ferroptosis.

    Evidence Astrocyte- and hepatocyte-specific conditional KO mice, K48-ubiquitination assays, RIPK1/RIPK3/MLKL necrosome readouts, DUB screens, site-specific lysine mutagenesis

    PMID:30709928 PMID:30944096 PMID:33712742 PMID:35296795 PMID:36271031

    Open questions at the time
    • Substrate prioritization across these pathways in a single cell type not addressed
  13. 2022 Medium

    Mapped post-translational regulation of OTUB1—Tyr26 phosphorylation enabling Raptor binding and Cys91 persulfidation enhancing E2 binding to stabilize SLC7A11—connecting redox and kinase inputs to OTUB1 output.

    Evidence Co-IP with Y26/Cys91 variants, in vitro kinase and persulfidation site-mapping, GLRX KO, ubiquitination and metabolic readouts; plus HIF-1α and GPX4 stabilization studies

    PMID:35732631 PMID:35927303 PMID:36369321 PMID:37703360

    Open questions at the time
    • Crosstalk among the many OTUB1 PTMs not integrated
    • Most are single-lab findings
  14. 2023 Medium

    Showed SET7 methylation at Lys122 selectively impairs the non-canonical UBC13-binding activity without affecting catalysis, providing a switch that derepresses ferroptosis, and extended substrate range to TGFBI, CCN6, and ERRα.

    Evidence In vitro methylation/K122 mutant, Co-IP, ROS/ferroptosis assays; MS substrate identification with site-specific mutagenesis and in vivo models

    PMID:36822329 PMID:36994729 PMID:37608493 PMID:39864589

    Open questions at the time
    • Whether methylation and other PTMs act combinatorially is untested
    • Single-lab substrate validations
  15. 2024 Medium

    Continued substrate expansion (CHK1, RACK1, YTHDF2) and revealed pathogen hijacking, with Legionella effectors ubiquitinating OTUB1 to redirect it to the bacterial vacuole and block its DUB activity.

    Evidence Co-IP, K48-ubiquitination assays, transcriptomic/m6A analysis, DNA-damage assays, reconstituted effector ubiquitination and infection models

    PMID:38266782 PMID:38315284 PMID:38462165 PMID:38818749

    Open questions at the time
    • Physiological relevance of effector-driven OTUB1 modification beyond infection unknown
    • Single-lab substrate findings

Open questions

Synthesis pass · forward-looking unresolved questions
  • How OTUB1 selects between canonical K48 cleavage and non-canonical E2 suppression for any given substrate in a given cellular context—and how its numerous PTMs are coordinated to set that choice—remains the central open question.
  • No unified model integrating PTM state, free-ubiquitin levels, and substrate selection
  • Most substrate relationships rest on single-lab studies

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 7 GO:0098772 molecular function regulator activity 5 GO:0016787 hydrolase activity 4
Localization
GO:0005634 nucleus 3 GO:0005739 mitochondrion 2 GO:0005886 plasma membrane 2 GO:0005829 cytosol 1
Pathway
R-HSA-168256 Immune System 6 R-HSA-5357801 Programmed Cell Death 5 R-HSA-73894 DNA Repair 5 R-HSA-162582 Signal Transduction 4 R-HSA-392499 Metabolism of proteins 4 R-HSA-1430728 Metabolism 3
Partners
C-IAP1DEPTORSLC7A11TRAF3TRAF6UBC13/UBE2NUBE2D/UBCH5UBE2E1

Evidence

Reading pass · 47 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2010 OTUB1 suppresses RNF168-dependent K63-linked poly-ubiquitination at DNA double-strand breaks independently of its catalytic deubiquitinase activity by binding to and inhibiting UBC13 (UBE2N), the cognate E2 enzyme for RNF168. OTUB1 also binds E2s of the UBE2D and UBE2E subfamilies through the same non-canonical mechanism. Co-immunoprecipitation, in vitro ubiquitination assays, siRNA knockdown with DSB repair readouts, catalytic mutant analysis Nature High 20725033
2012 Crystal structures of OTUB1–UBC13 and OTUB1 bound to ubiquitin aldehyde plus a chemical UBC13~Ub conjugate reveal that free ubiquitin allosterically binds a distal site on OTUB1, triggering conformational changes that form an N-terminal ubiquitin-binding helix, increase affinity for donor ubiquitin in the E2~Ub thiolester, occlude the RING E3 binding site on UBC13, and block acceptor-ubiquitin attack on the thiolester—thereby inhibiting K63-linked ubiquitin chain synthesis. X-ray crystallography, biochemical ubiquitination assays, structure-guided mutagenesis, surface plasmon resonance Nature High 22367539
2012 OTUB1 co-opts its canonical Lys48-linked distal ubiquitin-binding site to recognize charged E2~Ub thioester intermediates; free ubiquitin binding at this site promotes formation of the inhibited E2 complex, with the donor-ubiquitin Lys48 positioned near the OTUB1 catalytic site in a configuration mimicking K48-linked chain cleavage products. X-ray crystallography, biochemical E2 inhibition assays, mutagenesis, structural analysis Molecular cell High 22325355
2012 Crystal structure of human OTUB1 in complex with UBC13 and MMS2 at 3.15 Å resolution reveals atomic-level contacts required for inhibition of K63-linked ubiquitin chain formation; structure-based OTUB1 mutants fail to inhibit K63 chain formation in vitro and histone ubiquitination and 53BP1 foci in vivo. X-ray crystallography, surface plasmon resonance, structure-guided mutagenesis, in vitro ubiquitination assays, cell-based 53BP1 foci assay The Journal of biological chemistry High 22679021
2013 E2 ubiquitin-conjugating enzymes (UBC13, UBCH5) stimulate OTUB1 catalytic cleavage of K48-linked polyubiquitin by stabilizing folding of the OTUB1 N-terminal ubiquitin-binding helix; this stimulation is regulated by the ratio of charged to uncharged E2 and by concentrations of K48 polyubiquitin and free ubiquitin, establishing bidirectional cross-regulation between OTUB1 and its E2 partners. Biochemical DUB activity assays, X-ray crystallography of human and worm OTUB1/UBCH5B, mutagenesis Nature structural & molecular biology High 23955022
2009 OTUB1 interacts with TRAF3 and TRAF6 by co-immunoprecipitation and mediates their deubiquitination, thereby negatively regulating virus-triggered type I interferon induction and IRF3/NF-κB activation. Co-immunoprecipitation, overexpression/knockdown with reporter assays for IFN-β transcription, antiviral response assays The Journal of biological chemistry Medium 19996094
2009 OTUB1 directly deubiquitinates estrogen receptor alpha (ERα) in vitro and in cells; it interacts with ERα (identified by mass spectrometry of an ERα complex) and negatively regulates ERα-mediated transcription while stabilizing ERα protein on chromatin. Mass spectrometry of ERα complex, in vitro deubiquitination assay, Co-IP, transcriptional reporter assays, ChIP The Journal of biological chemistry Medium 19383985
2013 OTUB1 is recruited to the active phospho-SMAD2/3 complex upon TGFβ stimulation; it inhibits ubiquitylation and proteasomal degradation of phospho-SMAD2/3 via non-catalytic E2 inhibition (independent of its isopeptidase activity), thereby sustaining TGFβ-mediated gene transcription and cell migration. Depletion of OTUB1 causes rapid loss of phospho-SMAD2/3 rescued by the proteasomal inhibitor bortezomib. Co-immunoprecipitation (signal-dependent), siRNA knockdown, catalytic mutant analysis, proteasome inhibitor rescue, TGFβ reporter assays, migration assays Nature communications High 24071738
2013 OTUB1 associates with c-IAP1 within the TWEAK receptor signalling complex, disassembles K48-linked polyubiquitin chains from c-IAP1 in vitro and in vivo, stabilises c-IAP1, and thereby sustains TWEAK-induced canonical NF-κB and MAPK signalling while preventing caspase activation and cell death. Co-immunoprecipitation, in vitro and in vivo deubiquitination assays, siRNA knockdown with caspase/NF-κB readouts, zebrafish c-IAP knockdown model The EMBO journal High 23524849
2014 Otub1 suppresses MDM2-mediated p53 ubiquitination and stabilises p53 independently of its deubiquitinase activity by non-canonically inhibiting the E2 enzyme UbcH5; monoubiquitination of Otub1 (primarily at Lys59 and Lys109 by UbcH5) is required for Otub1 to suppress UbcH5 and stabilise p53, as a lysine-free Otub1 mutant (K0) fails to achieve these functions. In vitro ubiquitination/monoubiquitination assays, Co-IP, cycloheximide chase, apoptosis assays, cell proliferation assays, UbcH5 binding studies The Journal of biological chemistry High 24403071
2015 Casein kinase 2 (CK2) phosphorylates OTUB1 at Ser16, promoting its nuclear accumulation; pharmacological CK2 inhibition or genetic ablation blocks Ser16 phosphorylation and causes nuclear exclusion of OTUB1. Ser16-phosphorylated OTUB1 is detected exclusively in the nucleus and promotes 53BP1 DNA repair foci formation after ionising radiation. In vitro CK2 phosphorylation assay, phospho-specific antibody, CK2 inhibitor/KO, subcellular fractionation/immunofluorescence, 53BP1 foci assay Science signaling High 25872870
2015 OTUB1 inhibits ubiquitination and proteasomal degradation of FOXM1 through its catalytic (C91-dependent) deubiquitinase activity; the C91S catalytic mutant fails to stabilise FOXM1, increase its half-life, or enhance proliferative and epirubicin-resistance phenotypes. Co-immunoprecipitation, siRNA knockdown and overexpression, cycloheximide chase, catalytic mutant (C91S), K48-ubiquitin linkage analysis Oncogene High 26148240
2016 OTUB1 inhibits RAS mono- and di-ubiquitination independently of its catalytic activity, resulting in sequestration of RAS on the plasma membrane and enhanced RAS activation and ERK1/2 signalling, thereby promoting tumorigenesis in wild-type RAS cells. In vitro ubiquitination assays, catalytic mutant analysis, confocal microscopy for RAS membrane localisation, RAS-GTP pull-down, tumour xenograft assays EMBO molecular medicine High 26881969
2016 FIH (factor inhibiting HIF) hydroxylates OTUB1 at asparagine 22; mutation of N22 profoundly alters OTUB1 protein interaction network and impairs cellular metabolic processes, identifying OTUB1 as a non-HIF substrate whose activity is regulated by oxygen-dependent hydroxylation. In vitro hydroxylation assays, N22A mutant co-immunoprecipitation proteomics, cellular metabolic assays (oxygen consumption, glycolysis) PLoS biology Medium 26752685
2018 OTUB1 directly interacts with DEPTOR via its N-terminal domain, deubiquitinates DEPTOR in a Cys91-independent but Asp88-dependent (non-canonical) manner, stabilises DEPTOR, and thereby suppresses amino-acid-induced mTORC1 activation, cellular autophagy, and cell proliferation/size. In vitro deubiquitination assay, co-immunoprecipitation, catalytic-dead and D88A mutant analysis, mTORC1 activity assays (S6K phosphorylation), autophagy assays The Journal of biological chemistry High 29382726
2018 OTUB1 stabilises Snail by preventing its ubiquitination and proteasomal degradation, facilitating epithelial-mesenchymal transition and metastasis of esophageal squamous cell carcinoma cells. Co-immunoprecipitation, siRNA/overexpression, ubiquitination assays, invasion/migration assays Oncogene Medium 29559747
2018 OTUB1 non-canonically stabilises the E2 enzyme UBE2E1 by suppressing its autoubiquitination in vitro and in cells, preventing proteasomal degradation of UBE2E1; this function is independent of OTUB1 catalytic activity but requires its ability to bind UBE2E1. Otub1 knockout mice exhibit late-stage embryonic lethality. In vitro autoubiquitination assay, OTUB1 KO cell lines (mouse and human), cycloheximide chase, catalytic mutant and binding-defective mutant analysis, mouse knockout The Journal of biological chemistry High 30282802
2019 OTUB1 directly interacts with SLC7A11 (xCT), a key component of the cystine-glutamate antiporter, and stabilises SLC7A11 protein. CD44 overexpression enhances the OTUB1–SLC7A11 interaction, while CD44 depletion partially abrogates it. OTUB1 knockdown destabilises SLC7A11 and promotes ferroptosis in cancer xenografts. Biochemical purification/mass spectrometry to identify OTUB1 as SLC7A11-binding partner, Co-IP, siRNA knockdown, xenograft tumour growth assays, ferroptosis markers Cancer research High 30709928
2019 FIH forms an oxygen-sensitive, likely covalent (co-translational amide) bond with OTUB1 within cells, generating a heterotrimeric complex of two FIH and one OTUB1; this complexation regulates OTUB1 deubiquitinase activity in an oxygen-dependent manner, providing a mechanism for hypoxia adaptation. Biochemical co-precipitation, mass spectrometry analysis of bond, co-expression in mammalian and bacterial cells, DUB activity assays, oxygen-dependency experiments Redox biology Medium 31299612
2019 IL-15 induces membrane recruitment of Otub1, which then non-canonically inhibits ubiquitin-dependent AKT activation, setting a threshold for CD8+ T cell and NK cell activation; Otub1 deficiency causes AKT hyperactivation, enhanced T cell metabolic reprogramming, and augmented anti-cancer immunity. Conditional Otub1 KO mice, IL-15 stimulation assays, membrane fractionation, ubiquitination assays of AKT, T cell and NK cell functional assays, tumour challenge models Nature immunology High 31182807
2019 Otub1 stabilises p100 (NF-κB precursor) by preventing its ubiquitination and degradation; Otub1 deficiency causes both increased signal-induced p100 processing (non-canonical NF-κB) and steady-state p100 degradation (leading to aberrant canonical NF-κB activation), resulting in B-cell hyperplasia and lupus-like autoimmunity. B cell-conditional Otub1 KO mice, ubiquitination assays, NF-κB pathway activation readouts, immunophenotyping, autoimmunity assessment Cell research High 31086255
2019 The ubiquitin-like modifier FAT10 non-covalently interacts with OTUB1 and stimulates its K48-linked DUB activity; FAT10 also strengthens OTUB1's non-catalytic suppression of K63 polyubiquitylation on TRAF3 and increases OTUB1 interaction with UBCH5B, while covalent FAT10ylation leads to OTUB1 proteasomal degradation. In vitro DUB activity assays, Co-IP, ubiquitination assays of TRAF3, FAT10 covalent vs. non-covalent interaction analysis The Journal of biological chemistry Medium 30718280
2020 OTUB1 removes K48-linked ubiquitin chains from PD-L1 via its catalytic deubiquitinase activity, blocking ERAD-dependent PD-L1 degradation, stabilising PD-L1 at the tumour cell surface, and suppressing CD8+ T cell-mediated cytotoxicity. Co-immunoprecipitation, K48-ubiquitin chain-specific deubiquitination assays, OTUB1 KO/knockdown, PD-1 binding assay, PBMC cytotoxicity assay, in vivo tumour model with CD8+ T cell infiltration Cell death and differentiation High 33328570
2020 OTUB1 activates RIG-I antiviral signalling via a dual mechanism: hydrolysis of K48-linked polyubiquitin and formation of an E2-repressive complex with UBCH5c. Upon IAV infection, OTUB1 relocalises from the nucleus to mitochondrial membranes together with RIG-I. Influenza A NS1 protein triggers proteasomal degradation of OTUB1 to antagonise this signalling. Cell-free reconstitution system with [35S]IRF3, purified RIG-I, mitochondrial membranes, and OTUB1 variants; immunofluorescence for relocalization; OTUB1 CRISPR/knockdown with IFN readouts; NS1 degradation assay Cell reports High 32023470
2020 OTUB1 promotes NF-κB activity in dendritic cells through K48-linked deubiquitination and stabilisation of the E2 enzyme UBC13, which in turn increases K63-linked ubiquitination of IRAK1 and TRAF6, augmenting cytokine (IL-6, IL-12, TNF) production upon TLR stimulation. DC-specific Otub1 KO mice, ubiquitination assays for UBC13/IRAK1/TRAF6, cytokine measurement, T. gondii infection model Cellular & molecular immunology High 32024978
2020 Otub1 directly interacts with c-Maf and abrogates K48-linked polyubiquitination of c-Maf, preventing its degradation and enhancing its transcriptional activity; this deubiquitination depends on Otub1's Lys71 and N terminus but is independent of UBE2O. Mass spectrometry identification of Otub1-c-Maf interaction, Co-IP, K48-ubiquitination assays, deletion/point mutant analysis, myeloma cell survival assays Blood Medium 32842143
2021 OTUB1 inhibits MSH2 ubiquitination by blocking E2 ubiquitin transfer activity (non-canonical mechanism), maintaining MSH2 protein stability; OTUB1 depletion promotes MSH2 ubiquitination, increases mutation frequency, and causes resistance to genotoxic agents. Functional ubiquitination and deubiquitination assays, OTUB1 knockdown, mutation frequency assays, drug sensitivity assays The Journal of biological chemistry Medium 33640455
2021 OTUB1 stabilises SOCS1 via K48-linked deubiquitination, thereby inhibiting IFN-γ-induced JAK2/STAT1 signalling hyperactivation in astrocytes. Astrocyte-specific Otub1 KO mice develop more severe EAE due to astrocyte hyperactivation. Astrocyte-specific conditional Otub1 KO mice, K48-ubiquitination assays for SOCS1, JAK/STAT signalling readouts, EAE model The EMBO journal High 30944096
2021 OTUB1 prevents hepatocyte necroptosis by stabilising c-IAP1 through K48-deubiquitination, thereby maintaining K63-linked ubiquitination of RIPK1 and preventing RIPK1/RIPK3 necrosome formation and MLKL phosphorylation. OTUB1 deletion in liver parenchymal cells causes lethal necroptosis upon bacterial infection or TNF challenge. Hepatocyte-specific Otub1 KO mice (OTUB1LPC-KO), human HepG2 OTUB1-KO cells, K48-ubiquitination assay for c-IAP1, RIPK1 K63-ubiquitination, RIPK3/MLKL phosphorylation, necrostatin-1s rescue, MLKL KO rescue Cell death and differentiation High 33712742
2021 OTUB1 directly deubiquitinates YAP protein via its OTU domain at multiple lysine sites (K90, K280, K343, K494, K497), stabilising YAP and promoting Hippo pathway suppression in gastric cancer. DUB siRNA library screen, Co-IP, in vitro and in vivo deubiquitination assays, site-directed mutagenesis of YAP lysines Oncogene Medium 36271031
2021 OTUB1 deubiquitinates and stabilises MYC at the K323 site, preventing its proteasomal degradation; stabilised MYC promotes HK2 expression and aerobic glycolysis in breast cancer. OTUB1-mediated MYC stability is confirmed in OTUB1-knockout mice. Co-IP, in vitro deubiquitination assay, site-directed mutagenesis (K323), OTUB1-KO mice, glycolysis assays, in vivo tumorigenesis Cell death and differentiation High 35296795
2022 CST1 recruits OTUB1 to GPX4, relieving GPX4 ubiquitination and improving its protein stability; this reduces intracellular ROS and inhibits ferroptosis in gastric cancer cells. The CST1–OTUB1–GPX4 interaction was confirmed by Co-IP combined with mass spectrometry. Co-immunoprecipitation combined with mass spectrometry, ubiquitination assay, siRNA knockdown, ROS assays, in vivo metastasis models Oncogene Medium 36369321
2022 OTUB1 stabilises HIF-1α under hypoxia via non-canonical inhibition of K48-linked polyubiquitination (independent of PHDs/VHL/FIH); OTUB1 depletion reduces endogenous HIF-1α protein and suppresses hypoxia-induced glycolytic reprogramming. Co-IP, K48-ubiquitination assay, OTUB1 knockdown under hypoxia, HIF-1α protein stability assays, glycolysis metabolic assays Cell death & disease Medium 35732631
2022 OTUB1 non-canonically stabilises Raptor (mTORC1 component) by preventing its ubiquitination; phosphorylation of OTUB1 at Tyr26 by Src and SRMS kinases is required for the OTUB1–Raptor interaction, and dephosphorylation of OTUB1 at Y26 destabilises Raptor and sensitises cancer cells to anti-cancer drugs via mitochondrial ROS-mediated dysfunction. Biochemical interaction assays, Co-IP with Y26 mutant, in vitro kinase assays (Src/SRMS), ubiquitination assays of Raptor, drug sensitivity assays, mitochondrial ROS measurement Cell death and differentiation Medium 35927303
2022 Endogenous H2S (produced by CTH) persulfidates OTUB1 at cysteine 91, promoting OTUB1 interaction with the E2 UBCH5A and thereby decreasing ubiquitination and proteasomal degradation of SLC7A11/xCT, increasing cellular glutathione; GLRX reverses this by deglutathionylation (at C23 or C204) of OTUB1. S-glutathionylation/persulfidation site-mapping, Co-IP of OTUB1-UBCH5A, ubiquitination and stability assays of SLC7A11, GLRX knockout/overexpression, GSH measurement Science advances Medium 37703360
2023 SET7 lysine monomethylase directly interacts with OTUB1 and catalyses its methylation at lysine 122; this modification does not affect OTUB1's DUB catalytic activity but impairs its non-canonical ability to bind UBC13, thereby relieving OTUB1's suppressive role on ferroptosis. In vitro methylation assay, site-directed mutagenesis (K122), Co-IP of OTUB1–UBC13, cell viability and intracellular ROS assays The Journal of biological chemistry Medium 36822329
2023 OTUB1 non-canonically stabilises TGFBI by deubiquitinating it at K22 and K25 residues in a manner independent of OTUB1 catalytic activity; OTUB1-mediated TGFBI stabilisation promotes glycolysis and angiogenesis in infantile hemangioma. Mass spectrometry for substrate identification, Co-IP, ubiquitination assay with site-specific K22/K25 mutagenesis, catalytic mutant analysis, extracellular acidification rate assays, in vivo IH model Arteriosclerosis, thrombosis, and vascular biology Medium 36994729
2024 OTUB1 deubiquitinates and stabilises CHK1, enhancing DNA damage repair capacity and conferring radioresistance in lung cancer; OTUB1 depletion impairs DNA damage repair and overcomes radioresistance. Co-immunoprecipitation, Western blotting of CHK1 ubiquitination, OTUB1 knockdown with comet assay and immunofluorescence for DNA damage, RNA sequencing, in vivo xenograft with radiotherapy International journal of radiation oncology, biology, physics Medium 38266782
2024 OTUB1 non-canonically stabilises RACK1 via inhibition of its K48-linked ubiquitination (independent of catalytic activity), activating PI3K/AKT and FAK/ERK signalling in hepatocellular carcinoma; OTUB1 transcription is driven by the transcription factor MAZ at the OTUB1 promoter. Immunoprecipitation-tandem MS for RACK1 identification, Co-IP, K48-ubiquitination assays, catalytic-independent mechanism validation, ChIP-qPCR and dual-luciferase for MAZ-OTUB1 promoter regulation, in vivo xenograft Cellular oncology Medium 38315284
2024 OTUB1 non-canonically stabilises YTHDF2 by blocking ubiquitin transfer to it independent of deubiquitinase activity; stabilised YTHDF2 binds and promotes m6A-dependent degradation of PRSS8 mRNA, and PRSS8 loss decreases nuclear β-catenin via E-cadherin, promoting prostate cancer proliferation. In vitro and in vivo ubiquitination assays, Co-IP, m6A-seq and transcriptomic analysis, PRSS8 mRNA stability assays, mouse xenograft model The Journal of biological chemistry Medium 38462165
2024 Legionella pneumophila effectors SidC/SdcA ubiquitinate OTUB1 at multiple lysine residues, promoting its association with the Legionella-containing vacuole and interaction with DEPTOR, thereby suppressing MTORC1 signalling and promoting host autophagy; SidE family effectors also induce phosphoribosyl-linked ubiquitination of OTUB1 at Ser16/Ser18 to block its DUB activity. Biochemical ubiquitination assays with purified effectors, Co-IP of OTUB1-DEPTOR, mTORC1 activity readouts, autophagy assays, BMDM infection model Autophagy Medium 38818749
2014 HSCARG promotes OTUB1 recruitment to TRAF3, facilitating TRAF3 deubiquitination by OTUB1 and suppressing RIG-I-like receptor signalling; HSCARG knockout attenuates TRAF3 deubiquitination by OTUB1, and OTUB1 knockdown abolishes the HSCARG-mediated suppression of IFN-β transcription. Co-immunoprecipitation, ubiquitination assays of TRAF3, HSCARG KO and OTUB1 KD with IFN-β reporter assays, epistasis experiments PLoS pathogens Medium 24763515
2017 Otub1 stabilises MDMX via non-canonical inhibition of MDM2-mediated MDMX ubiquitination (independently of deubiquitinase activity); Otub1-stabilised MDMX localises to mitochondria, enhances p53 phosphorylation at S46, and promotes mitochondria-mediated apoptosis. Co-IP, in vitro ubiquitination assay, catalytic mutant analysis, subcellular fractionation, p53-S46 phosphorylation assay, apoptosis assays Oncotarget Medium 28035068
2021 OTUB1 suppresses K63-linked ubiquitination of TRAF6 via direct binding, thereby inhibiting the TRAF6-ASK1 signalling axis and protecting against NASH-associated hepatic steatosis, inflammation, and fibrosis. Immunoprecipitation-mass spectrometry for TRAF6 identification, Co-IP, K63-ubiquitination assay of TRAF6, hepatocyte-specific Otub1 overexpression mouse model (HFHC diet), Otub1 KO mice Hepatology Medium 34591986
2022 ERK/RSK-mediated phosphorylation of YB-1 at Ser102 in hyperglycaemia disrupts its interaction with OTUB1, leading to YB-1 ubiquitination and proteasomal degradation; when the ERK pathway is inhibited, YB-1–OTUB1 interaction is stabilised, preserving YB-1 levels and ameliorating diabetic cardiomyopathy. Co-immunoprecipitation, ubiquitination assay of YB-1, phospho-specific YB-1(S102) analysis, ERK inhibitor (U0126) in STZ-diabetic mice, echocardiography The Journal of biological chemistry Medium 35490780
2023 OTUB1 non-canonically stabilises CCN6 by inhibiting K48-linked ubiquitination via interaction through its linker domain; OTUB1 deletion reduces CCN6 abundance and increases breast cancer cell migration and proliferation, which is rescued by CCN6 supplementation. DUB screening assay, Co-IP, K48-ubiquitination assay, OTUB1 deletion mutants (linker domain), breast cancer cell functional assays, allograft model Clinical and translational medicine Medium 37608493
2025 OTUB1 is a novel deubiquitinating enzyme for ERRα; OTUB1 C91 residue is critical for ERRα deubiquitination. The natural product Ailanthone (AIL) inhibits OTUB1 activity by interacting with C91, disrupts the OTUB1–ERRα interaction, and promotes ERRα ubiquitination and proteasomal degradation. Competitive activity-based protein profiling (ABPP) for target identification, Co-IP, luciferase reporter assay, C91A mutant analysis, surface plasmon resonance, in vivo TNBC mouse model Journal of advanced research Medium 39864589

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2019 The Deubiquitylase OTUB1 Mediates Ferroptosis via Stabilization of SLC7A11. Cancer research 421 30709928
2010 Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1. Nature 312 20725033
2022 CST1 inhibits ferroptosis and promotes gastric cancer metastasis by regulating GPX4 protein stability via OTUB1. Oncogene 262 36369321
2012 The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature 213 22367539
2012 OTUB1 co-opts Lys48-linked ubiquitin recognition to suppress E2 enzyme function. Molecular cell 176 22325355
2009 Regulation of virus-triggered signaling by OTUB1- and OTUB2-mediated deubiquitination of TRAF3 and TRAF6. The Journal of biological chemistry 161 19996094
2020 Deubiquitinating enzyme OTUB1 promotes cancer cell immunosuppression via preventing ER-associated degradation of immune checkpoint protein PD-L1. Cell death and differentiation 153 33328570
2015 OTUB1 inhibits the ubiquitination and degradation of FOXM1 in breast cancer and epirubicin resistance. Oncogene 125 26148240
2013 OTUB1 enhances TGFβ signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3. Nature communications 118 24071738
2013 E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Nature structural & molecular biology 108 23955022
2017 Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield. Cell research 105 28776570
2013 OTUB1 modulates c-IAP1 stability to regulate signalling pathways. The EMBO journal 102 23524849
2022 m6A demethylase FTO renders radioresistance of nasopharyngeal carcinoma via promoting OTUB1-mediated anti-ferroptosis. Translational oncology 90 36343416
2019 The deubiquitinase Otub1 controls the activation of CD8+ T cells and NK cells by regulating IL-15-mediated priming. Nature immunology 90 31182807
2018 OTUB1 promotes esophageal squamous cell carcinoma metastasis through modulating Snail stability. Oncogene 87 29559747
2016 OTUB1 triggers lung cancer development by inhibiting RAS monoubiquitination. EMBO molecular medicine 87 26881969
2022 Deubiquitination of MYC by OTUB1 contributes to HK2 mediated glycolysis and breast tumorigenesis. Cell death and differentiation 84 35296795
2016 FIH Regulates Cellular Metabolism through Hydroxylation of the Deubiquitinase OTUB1. PLoS biology 83 26752685
2009 OTU Domain-containing ubiquitin aldehyde-binding protein 1 (OTUB1) deubiquitinates estrogen receptor (ER) alpha and affects ERalpha transcriptional activity. The Journal of biological chemistry 76 19383985
2020 The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13. Cellular & molecular immunology 64 32024978
2022 OTUB1 suppresses Hippo signaling via modulating YAP protein in gastric cancer. Oncogene 63 36271031
2012 Molecular basis of Lys-63-linked polyubiquitination inhibition by the interaction between human deubiquitinating enzyme OTUB1 and ubiquitin-conjugating enzyme UBC13. The Journal of biological chemistry 62 22679021
2020 OTUB1 Is a Key Regulator of RIG-I-Dependent Immune Signaling and Is Targeted for Proteasomal Degradation by Influenza A NS1. Cell reports 59 32023470
2015 OTUB1 de-ubiquitinating enzyme promotes prostate cancer cell invasion in vitro and tumorigenesis in vivo. Molecular cancer 57 25623341
2015 Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization. Science signaling 57 25872870
2021 Deubiquitinating enzyme OTUB1 in immunity and cancer: Good player or bad actor? Cancer letters 55 34875341
2017 miR-542-3p inhibits colorectal cancer cell proliferation, migration and invasion by targeting OTUB1. American journal of cancer research 55 28123857
2014 HSCARG negatively regulates the cellular antiviral RIG-I like receptor signaling pathway by inhibiting TRAF3 ubiquitination via recruiting OTUB1. PLoS pathogens 55 24763515
2018 OTUB1 protein suppresses mTOR complex 1 (mTORC1) activity by deubiquitinating the mTORC1 inhibitor DEPTOR. The Journal of biological chemistry 53 29382726
2021 Targeting the Otub1/c-Maf axis for the treatment of multiple myeloma. Blood 51 32842143
2019 OTUB1 inhibits CNS autoimmunity by preventing IFN-γ-induced hyperactivation of astrocytes. The EMBO journal 47 30944096
2014 Deubiquitinating enzyme regulation of the p53 pathway: A lesson from Otub1. World journal of biological chemistry 47 24920999
2014 Monoubiquitination is critical for ovarian tumor domain-containing ubiquitin aldehyde binding protein 1 (Otub1) to suppress UbcH5 enzyme and stabilize p53 protein. The Journal of biological chemistry 46 24403071
2021 The ubiquitin hydrolase OTUB1 promotes glioma cell stemness via suppressing ferroptosis through stabilizing SLC7A11 protein. Bioengineered 45 34927544
2019 Preventing abnormal NF-κB activation and autoimmunity by Otub1-mediated p100 stabilization. Cell research 42 31086255
2021 Endogenous hydrogen sulfide regulates xCT stability through persulfidation of OTUB1 at cysteine 91 in colon cancer cells. Neoplasia (New York, N.Y.) 40 33878705
2021 OTUB1 prevents lethal hepatocyte necroptosis through stabilization of c-IAP1 during murine liver inflammation. Cell death and differentiation 39 33712742
2022 Mutant p53 driven-LINC00857, a protein scaffold between FOXM1 and deubiquitinase OTUB1, promotes the metastasis of pancreatic cancer. Cancer letters 37 36272615
2021 OTUB1 alleviates NASH through inhibition of the TRAF6-ASK1 signaling pathways. Hepatology (Baltimore, Md.) 37 34591986
2023 Glutaredoxin attenuates glutathione levels via deglutathionylation of Otub1 and subsequent destabilization of system xC. Science advances 36 37703360
2018 OTUB1 non-catalytically stabilizes the E2 ubiquitin-conjugating enzyme UBE2E1 by preventing its autoubiquitination. The Journal of biological chemistry 36 30282802
2021 OTUB1 facilitates bladder cancer progression by stabilizing ATF6 in response to endoplasmic reticulum stress. Cancer science 35 33686769
2017 Expression of OTUB1 in hepatocellular carcinoma and its effects on HCC cell migration and invasion. Acta biochimica et biophysica Sinica 35 28575188
2017 Otub1 stabilizes MDMX and promotes its proapoptotic function at the mitochondria. Oncotarget 34 28035068
2019 SP1-mediated overexpression of lncRNA LINC01234 as a ceRNA facilitates non-small-cell lung cancer progression via regulating OTUB1. Journal of cellular physiology 33 31106421
2020 OTUB1-mediated deubiquitination of FOXM1 up-regulates ECT-2 to promote tumor progression in renal cell carcinoma. Cell & bioscience 30 32257108
2014 Colon cancer bears overexpression of OTUB1. Pathology, research and practice 30 24947413
2017 Silencing of OTUB1 inhibits migration of human glioma cells in vitro. Neuropathology : official journal of the Japanese Society of Neuropathology 29 28139865
2021 OTUB1 stabilizes mismatch repair protein MSH2 by blocking ubiquitination. The Journal of biological chemistry 28 33640455
2020 Amyloid aggregates of the deubiquitinase OTUB1 are neurotoxic, suggesting that they contribute to the development of Parkinson's disease. The Journal of biological chemistry 28 32005664
2019 The ubiquitin-like modifier FAT10 stimulates the activity of deubiquitylating enzyme OTUB1. The Journal of biological chemistry 27 30718280
2021 Inhibition of the Otub1/c-Maf axis by the herbal acevaltrate induces myeloma cell apoptosis. Cell communication and signaling : CCS 25 33627137
2020 Activation and selectivity of OTUB-1 and OTUB-2 deubiquitinylases. The Journal of biological chemistry 25 32265297
2019 Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF3 ubiquitination through recruiting OTUB1. The FEBS journal 24 31386800
2021 OTUB1 Promotes Progression and Proliferation of Prostate Cancer via Deubiquitinating and Stabling Cyclin E1. Frontiers in cell and developmental biology 22 33537306
2021 OTUB1 regulates lung development, adult lung tissue homeostasis, and respiratory control. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22 34793600
2022 ERK/RSK-mediated phosphorylation of Y-box binding protein-1 aggravates diabetic cardiomyopathy by suppressing its interaction with deubiquitinase OTUB1. The Journal of biological chemistry 21 35490780
2019 MicroRNA‑542‑3p represses OTUB1 expression to inhibit migration and invasion of esophageal cancer cells. Molecular medicine reports 21 31939620
2024 β-Elemene promotes ferroptosis and reverses radioresistance in gastric cancer by inhibiting the OTUB1-GPX4 interaction. Frontiers in pharmacology 20 39484165
2022 OTUB1 augments hypoxia signaling via its non-canonical ubiquitination inhibition of HIF-1α during hypoxia adaptation. Cell death & disease 20 35732631
2024 CircSEC24B activates autophagy and induces chemoresistance of colorectal cancer via OTUB1-mediated deubiquitination of SRPX2. Cell death & disease 19 39333496
2022 Phosphorylation of OTUB1 at Tyr 26 stabilizes the mTORC1 component, Raptor. Cell death and differentiation 19 35927303
2022 Discovery of Potent OTUB1/USP8 Dual Inhibitors Targeting Proteostasis in Non-Small-Cell Lung Cancer. Journal of medicinal chemistry 19 36221183
2019 Oxygen-dependent bond formation with FIH regulates the activity of the client protein OTUB1. Redox biology 19 31299612
2019 ERRα Regulates OTUB1 Expression to Promote Colorectal Cancer Cell Migration. Journal of Cancer 19 31737118
2024 OTUB1-mediated inhibition of ubiquitination: a growing list of effectors, multiplex mechanisms, and versatile functions. Frontiers in molecular biosciences 18 38264570
2023 OTUB1 inhibits breast cancer by non-canonically stabilizing CCN6. Clinical and translational medicine 18 37608493
2021 Activated Protein C Ameliorates Diabetic Cardiomyopathy via Modulating OTUB1/YB-1/MEF2B Axis. Frontiers in cardiovascular medicine 18 34778410
2024 OTUB1 regulation of ferroptosis and the protective role of ferrostatin-1 in lupus nephritis. Cell death & disease 17 39500879
2023 Deubiquitinase OTUB1 regulates doxorubicin-induced cardiotoxicity via deubiquitinating c-MYC. Cellular signalling 17 37871668
2021 The Long Non-coding RNA TMPO-AS1 Promotes Bladder Cancer Growth and Progression via OTUB1-Induced E2F1 Deubiquitination. Frontiers in oncology 17 33816295
2021 Inhibition of cathepsin K sensitizes oxaliplatin-induced apoptotic cell death by Bax upregulation through OTUB1-mediated p53 stabilization in vitro and in vivo. Oncogene 17 34785775
2023 SET7 methylates the deubiquitinase OTUB1 at Lys 122 to impair its binding to E2 enzyme UBC13 and relieve its suppressive role on ferroptosis. The Journal of biological chemistry 16 36822329
2024 OTUB1 Targets CHK1 for Deubiquitination and Stabilization to Facilitate Lung Cancer Progression and Radioresistance. International journal of radiation oncology, biology, physics 15 38266782
2024 TCF12 induces ferroptosis by suppressing OTUB1-mediated SLC7A11 deubiquitination to promote cisplatin sensitivity in oral squamous cell carcinoma. Cell biology international 15 38946134
2022 The Deubiquitinase OTUB1 Is a Key Regulator of Energy Metabolism. International journal of molecular sciences 15 35163456
2018 Moderate-Intensity Exercise Induces Neurogenesis and Improves Cognition in Old Mice by Upregulating Hippocampal Hippocalcin, Otub1, and Spectrin-α. Molecular neurobiology 15 30091036
2016 OTUB1 attenuates neuronal apoptosis after intracerebral hemorrhage. Molecular and cellular biochemistry 15 27629786
2010 When cleavage is not attractive: non-catalytic inhibition of ubiquitin chains at DNA double-strand breaks by OTUB1. DNA repair 14 21130715
2024 OTUB1 accelerates hepatocellular carcinoma by stabilizing RACK1 via its non-canonical ubiquitination. Cellular oncology (Dordrecht, Netherlands) 13 38315284
2020 Anti-bacterial and anti-viral nanchangmycin displays anti-myeloma activity by targeting Otub1 and c-Maf. Cell death & disease 13 32999280
2024 Bacterial ubiquitin ligases hijack the host deubiquitinase OTUB1 to inhibit MTORC1 signaling and promote autophagy. Autophagy 12 38818749
2022 S-Nitrosylation of OTUB1 Alters Its Stability and Ubc13 Binding. ACS chemical neuroscience 12 35500217
2021 The deubiquitinase OTUB1 fosters papillary thyroid carcinoma growth through EYA1 stabilization. Journal of cellular and molecular medicine 12 34773364
2008 Partial molecular cloning, characterization, and analysis of the subcellular localization and expression patterns of the porcine OTUB1 gene. Molecular biology reports 12 18781400
2023 OTUB1 Catalytic-Independently Deubiquitinates TGFBI and Mediates the Angiogenesis in Infantile Hemangioma by Regulating Glycolysis. Arteriosclerosis, thrombosis, and vascular biology 11 36994729
2025 Ailanthone induces triple-negative breast cancer cells death involving the inhibition of OTUB1-mediated ERRα deubiquitylation. Journal of advanced research 10 39864589
2020 MicroRNA-103a Curtails the Stemness of Non-Small Cell Lung Cancer Cells by Binding OTUB1 via the Hippo Signaling Pathway. Technology in cancer research & treatment 10 33174524
2024 YTHDF2 protein stabilization by the deubiquitinase OTUB1 promotes prostate cancer cell proliferation via PRSS8 mRNA degradation. The Journal of biological chemistry 9 38462165
2024 OTUB1/NDUFS2 axis promotes pancreatic tumorigenesis through protecting against mitochondrial cell death. Cell death discovery 9 38653740
2024 Vibrio splendidus infection promotes circRNA-FGL1-regulated coelomocyte apoptosis via competitive binding to Myc with the deubiquitinase OTUB1 in Apostichopus japonicus. PLoS pathogens 9 39146353
2024 Targeting and degradation of OTUB1 by Erianin for antimetastasis in esophageal squamous cell carcinoma. Phytomedicine : international journal of phytotherapy and phytopharmacology 9 39566402
2023 An indispensable role of TAZ in anoikis resistance promoted by OTUB1 deubiquitinating enzyme in basal-like triple-negative breast cancer cells. Biochemical and biophysical research communications 9 36738577
2023 OTUB1's role in promoting OSCC development by stabilizing RACK1 involves cell proliferation, migration, invasion, and tumor-associated macrophage M1 polarization. Cellular signalling 9 37532135
2022 Role of the OTUB1/IRF7/NOX4 axis in oxidative stress injury and inflammatory responses in mice with Parkinson's disease. Psychogeriatrics : the official journal of the Japanese Psychogeriatric Society 9 36332656
2012 OTUB1 overexpression in mesangial cells is a novel regulator in the pathogenesis of glomerulonephritis through the decrease of DCN level. PloS one 9 22279542
2025 Primary Cilia Formation Mediated by Hsa_Circ_0005185/OTUB1/RAB8A Complex Inhibits Prostate Cancer Progression by Suppressing Hedgehog Signaling Pathway. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8 39785769
2024 Target deubiquitinase OTUB1 as a therapeatic strategy for BLCA via β-catenin/necroptosis signal pathway. International journal of biological sciences 8 39113709
2023 The deubiquitinase OTUB1 governs lung cancer cell fitness by modulating proteostasis of OXPHOS proteins. Biochimica et biophysica acta. Molecular basis of disease 8 37245529
2020 Comparison of Cross-Regulation by Different OTUB1:E2 Complexes. Biochemistry 8 32049508

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