Affinage

NUTF2

Nuclear transport factor 2 · UniProt P61970

Length
127 aa
Mass
14.5 kDa
Annotated
2026-06-10
56 papers in source corpus 26 papers cited in narrative 27 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NUTF2 (NTF2) is a small homodimeric nuclear transport factor that serves as the dedicated import receptor for RanGDP, replenishing the nuclear Ran pool that powers all importin-β-family transport (PMID:7744965, PMID:9822603). Originally purified as a cytosolic factor acting at a late, post-docking stage of nuclear protein import through its interaction with the nucleoporin p62 (PMID:7744965), NTF2 binds specifically to the GDP-bound form of Ran but not RanGTP, and engages FxFG-repeat nucleoporins at a separate, non-competing site on the dimer (PMID:8918934, PMID:8755535). Crystallographic and structure-guided mutagenesis studies define both interfaces: the Ran switch II loop (Phe72, Lys71, Arg76) inserts into NTF2's hydrophobic cavity, with salt bridges to NTF2 Asp92/Asp94/Glu42 conferring GDP-versus-GTP selectivity, while a distinct dimer-interface site formed from both chains binds FxFG nucleoporins (PMID:9368653, PMID:9533885, PMID:12065398). Mutations that abolish Ran binding (E42K, D92N/D94N) or nucleoporin binding (W7A) each fail to support RanGDP import, establishing that both contacts are required for facilitated translocation through the pore (PMID:9368653, PMID:10543952). The receptor functions directionally: it imports RanGDP, docks at and rapidly transits NPCs, and releases Ran upon RCC1-catalyzed exchange to RanGTP, for which it has no affinity (PMID:9822603, PMID:12189172); NTF2 additionally acts as a Ran-GDI that stabilizes the GDP-bound state by blocking nucleotide dissociation (PMID:9843686). This activity is essential for viability and maintains the nuclear Ran concentration in living cells, linking Ran import to cell cycle progression through a spindle-assembly-checkpoint-dependent arrest when disrupted (PMID:8702493, PMID:10679025, PMID:10930458). Beyond transport, NTF2 concentration negatively regulates nuclear size by reducing NPC diameter in a Ran-binding-dependent manner and selectively limiting import of large cargo (PMID:20946986, PMID:26823604).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 1995 High

    Established that a distinct cytosolic factor is required for nuclear protein import downstream of initial ligand docking, defining NTF2 as a discrete transport component rather than part of the importin machinery.

    Evidence Biochemical complementation of depleted cytosol with recombinant protein rescue and flow-cytometry import assay

    PMID:7744965

    Open questions at the time
    • Molecular cargo and the precise transport step were not defined
    • No structural basis for nucleoporin interaction
  2. 1996 High

    Solved the NTF2 fold and identified its hydrophobic cavity, then showed nucleotide-specific binding to RanGDP and separate FxFG-nucleoporin binding, revealing the bipartite molecular logic of the receptor.

    Evidence X-ray crystallography at 1.6 Å, affinity pulldowns from liver homogenate, competition binding, and GDP/GTP-loaded Ran binding assays; yeast essentiality and human complementation

    PMID:8702493 PMID:8755535 PMID:8757804 PMID:8918934

    Open questions at the time
    • Atomic basis of GDP-vs-GTP selectivity not yet resolved at the complex level
    • Cargo of NTF2 transport not yet identified as Ran itself
  3. 1997 High

    Demonstrated by structure-guided mutagenesis that the Ran-binding cavity is functionally separable from nucleoporin binding and is strictly required for import and viability, validating the two-site model in vivo.

    Evidence Site-directed mutants (E42K, D92N/D94N), mutant crystal structure, permeabilized-cell import, and yeast viability assays

    PMID:9368653

    Open questions at the time
    • Direction and recycling of the receptor not yet established
  4. 1998 High

    Resolved the GDP-Ran–NTF2 co-crystal to explain GDP/GTP selectivity at atomic resolution and established NTF2 as the directional import receptor for RanGDP that also acts as a Ran-GDI.

    Evidence 2.5 Å co-crystal structure, in vitro import reconstitution with loss-of-function mutants, digitonin-permeabilized assays, dominant-negative importin-β, and radiolabeled nucleotide dissociation assays

    PMID:9533885 PMID:9822603 PMID:9843686 PMID:9889103

    Open questions at the time
    • Mechanism of RanGDP release on the nuclear side not directly characterized
    • How NTF2 recycles back to the cytoplasm unresolved
  5. 1999 High

    Established that both the Ran switch II residues and the NTF2 nucleoporin-binding tryptophan are individually essential for import, mechanistically coupling FG-nucleoporin engagement to productive translocation.

    Evidence Crystal structures of W7A-NTF2 and Ran switch II mutants, fluorescence/permeabilized import assays, Xenopus oocyte microinjection with colloidal gold EM, yeast viability

    PMID:10356329 PMID:10543952

    Open questions at the time
    • Full set of FG-binding sites on NTF2 not yet mapped
  6. 2000 High

    Showed in living cells and yeast that NTF2 maintains the nuclear Ran concentration, and that loss of nuclear Ran arrests the cell cycle through the spindle-assembly checkpoint, connecting Ran import to proliferation.

    Evidence Anti-NTF2 mAb cytoplasmic microinjection, fluorescence microscopy, in vitro import; yeast ts genetics with MAD2 epistasis

    PMID:10679025 PMID:10930458

    Open questions at the time
    • Direct mechanism linking Ran depletion to checkpoint activation not dissected
  7. 2002 Medium

    Defined the symmetric dual FxFG-binding sites on the dimer and measured facilitated NTF2 transit through the NPC, and identified a non-FG nucleoporin (DNup88) partner in Drosophila tied to NF-κB nuclear import.

    Evidence 1.9 Å co-crystal with FxFG core plus yeast functional validation, optical single-transporter recording in isolated nuclei, Drosophila genetics with Rel-protein localization and direct interaction

    PMID:11943764 PMID:12065398 PMID:12189172

    Open questions at the time
    • DNup88 interaction characterized in a single organism
    • Single-transporter measurements from one study
  8. 2004 Medium

    Identified additional regulatory layers: an ATP-dependent phosphorylation-sensitive activity that displaces RanGDP from NTF2, and a conserved non-FxFG NPC-binding surface, refining the model of receptor cycling.

    Evidence In vitro GDP-dissociation assays with hydrolyzable vs non-hydrolyzable ATP and phosphatase inhibitors; evolutionary analysis with D23A NPC-binding mutant

    PMID:15155737 PMID:15522285

    Open questions at the time
    • Identity of the ATP-dependent displacement factor unknown
    • Physiological relevance of the second NPC-binding site not established in vivo
  9. 2010 High

    Revealed a developmental and morphometric role: NTF2 concentration scales inversely with nuclear size and selectively limits large-cargo import, accounting for interspecies nuclear-size differences via lamin B3 import.

    Evidence Xenopus egg extract nuclear reconstitution with titration of NTF2 and importin-α, fluorescent import assays

    PMID:20946986

    Open questions at the time
    • Mechanism by which NTF2 levels constrain import not yet defined at the pore
  10. 2016 High

    Showed that NTF2 controls nuclear size by reducing NPC diameter in a Ran-binding-dependent manner, mechanistically grounding the size phenotype in pore architecture.

    Evidence Xenopus egg/embryo extract reconstitution with Ran-binding-deficient NTF2 mutants, NPC diameter measurement, ectopic expression in mammalian cells

    PMID:26823604

    Open questions at the time
    • How Ran binding to NTF2 alters NPC diameter molecularly is unresolved
  11. 2024 Medium

    Probed the physical nature of the FG-Nup–NTF2 complex, showing FG repeats remain disordered upon binding with no inter-aromatic crosslinking, informing models of how the receptor transits the permeability barrier.

    Evidence Small-angle neutron scattering with site-specific deuteration (preprint)

    Open questions at the time
    • Preprint, single study
    • In vitro reconstitution may not capture the crowded NPC environment

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NTF2 recycling, post-translational regulation, and putative signaling-linked moonlighting functions integrate with its canonical RanGDP import role remains unresolved.
  • Identity of the ATP/phosphorylation-dependent displacement factor unknown
  • Reported EGFR/ER-signaling and senescence roles rest on low-confidence or preprint evidence
  • Mechanism coupling Ran binding to NPC diameter change undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140104 molecular carrier activity 3 GO:0060089 molecular transducer activity 2 GO:0098772 molecular function regulator activity 2
Localization
GO:0005634 nucleus 1 GO:0005635 nuclear envelope 1 GO:0005829 cytosol 1
Pathway
R-HSA-1852241 Organelle biogenesis and maintenance 2 R-HSA-9609507 Protein localization 2 R-HSA-1640170 Cell Cycle 1
Partners
NUP62NUP88RAN
Complex memberships
NTF2 homodimerNTF2–RanGDP complex

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 NTF2 (NUTF2) was purified as a cytosolic factor that interacts with the nuclear pore complex glycoprotein p62; it exists as an apparent dimer of ~14-kDa subunits and acts at a relatively late stage of nuclear protein import, subsequent to initial docking of import ligand at the nuclear envelope. Biochemical complementation of depleted cytosol, flow cytometry-based import assay, recombinant protein rescue The Journal of cell biology High 7744965
1996 X-ray crystallography of rat NTF2 at 1.6 Å resolution revealed an alpha+beta barrel fold with a distinctive hydrophobic cavity homologous to scytalone dehydratase; the cavity contains a putative catalytic Asp-His pair and is a candidate binding site for Ran and nucleoporin p62. X-ray crystallography Journal of molecular biology High 8757804
1996 NTF2 binds specifically to RanGDP (but not RanGTP) and to xFxFG repeat-containing domains of nucleoporins p62 and Nsp1p at separate, non-competing binding sites on the dimer. Affinity pulldown (Sepharose-bound NTF2) from rat liver homogenates; competition binding assays Journal of molecular biology High 8918934
1996 The NTF2 gene is essential for viability in S. cerevisiae; temperature-sensitive ntf2 mutants show defects in nuclear protein localization but not poly(A)+ RNA export, and NTF2 protein concentrates at the nuclear envelope. Yeast genetics (conditional lethal mutants), epitope-tagged localization, human gene complementation The Journal of biological chemistry High 8702493
1996 NTF2 nucleotide-specifically binds the GDP-bound form of Ran/TC4 (not GTP-bound), as demonstrated by solution and solid-phase binding assays with radiolabeled nucleotide-preloaded Ran. Solution and solid-phase binding assays with [γ-32P]GTP- and [3H]GDP-loaded Ran Proceedings of the National Academy of Sciences of the United States of America High 8755535
1997 Site-directed NTF2 mutants (E42K, D92N/D94N) in conserved residues surrounding the hydrophobic cavity lose GDP-Ran binding while retaining FxFG-nucleoporin binding; these mutants fail to support nuclear protein import in permeabilized cells and are non-viable when substituted for chromosomal yeast NTF2. Site-directed mutagenesis, crystal structure of E42K mutant, permeabilized-cell import assay, yeast viability assay Journal of molecular biology High 9368653
1998 Crystal structure of the GDP-Ran–NTF2 complex at 2.5 Å resolution showed that the switch II loop of Ran (residues 65–78) interacts with the hydrophobic cavity of NTF2; Phe72 inserts into the cavity, and salt bridges between Ran Lys71/Arg76 and NTF2 Asp92/Asp94/Glu42 account for GDP-vs-GTP selectivity. X-ray crystallography of the complex Journal of molecular biology High 9533885
1998 NTF2 acts as the transport receptor that mediates nuclear import of RanGDP; it binds RanGDP, docks at NPCs, and translocates the NTF2–RanGDP complex to the nuclear side, where RanGDP is released upon nucleotide exchange to RanGTP (to which NTF2 has no detectable affinity), establishing directionality of Ran import. In vitro nuclear import reconstitution with purified factors, point-mutant NTF2 that cannot bind RanGDP, digitonin-permeabilized cell assay The EMBO journal High 9822603
1998 NTF2-mediated nuclear import of RanGDP is sufficient (with energy) to drive Ran nuclear accumulation; a dominant-negative importin-beta mutant inhibits Ran import, and a RanGTP-locked mutant (unable to bind NTF2) is not imported. Digitonin-permeabilized cell nuclear import assay, NTF2 mutant unable to bind Ran, dominant-negative importin-beta Current biology : CB High 9889103
1998 NTF2 acts as a GDP dissociation inhibitor (Ran-GDI) for Ran: it dramatically inhibits dissociation of GDP from Ran and blocks subsequent GTP binding catalyzed by RCC1, without affecting GTP dissociation; the GDI activity of NTF2 mutants correlates with their nuclear import activity. Radiolabeled nucleotide dissociation assays ([3H]GDP, [35S]GTPγS) with recombinant proteins, permeabilized-cell import assay with NTF2 mutants Current biology : CB High 9843686
1998 The RanQ69L mutant fails to bind NTF2 because the Q69L substitution causes a large conformational change in the switch II loop (residues 68–74), repositioning Lys71, Phe72, and Arg76 that are critical for the Ran–NTF2 interface. X-ray crystallography of GDP-RanQ69L (2.3 Å), solution binding assays Journal of molecular biology High 9878368
1999 Interaction between NTF2 and xFxFG-containing nucleoporins is required for nuclear import of RanGDP: the W7A mutation in NTF2 specifically reduces nucleoporin affinity while retaining RanGDP binding, and W7A-NTF2 only weakly stimulates RanGDP import in permeabilized cells and binds less strongly to the NPC central channel. Crystal structure of W7A-NTF2 (2.5 Å), fluorescence import assay, nuclear envelope binding assay, microinjection into Xenopus oocytes with colloidal gold EM Journal of molecular biology High 10543952
1999 Switch II residues Lys71, Phe72, and Arg76 of Ran are all individually essential for interaction with NTF2; mutations F72Y, F72W, and R76E abolish NTF2 binding without major structural change, and these Ran mutants are deficient in stimulating nuclear protein import. X-ray crystallography of Ran switch II mutants, solution binding assays, permeabilized-cell import assay, yeast viability assay (Gsp1p equivalents) Journal of molecular biology High 10356329
2000 Cytoplasmic injection of anti-NTF2 monoclonal antibodies causes dramatic relocalization of Ran to the cytoplasm in vivo and inhibits nuclear import of both Ran and NLS-containing proteins in vitro, demonstrating that NTF2 is required to maintain the nuclear concentration of Ran in living cells. Cytoplasmic microinjection of anti-NTF2 mAbs, fluorescence microscopy, in vitro import assay Molecular biology of the cell High 10679025
2000 In S. cerevisiae, a temperature-sensitive NTF2 mutant that cannot bind Ran fails to import Ran into the nucleus at non-permissive temperature; depletion of nuclear Ran triggers a MAD2 spindle-assembly checkpoint-dependent G2 arrest, linking NTF2-mediated Ran import to cell cycle progression. Yeast temperature-sensitive genetics, Ran localization assay, epistasis with MAD2 deletion Molecular biology of the cell High 10930458
2002 1.9 Å crystal structure of yeast NTF2-N77Y bound to an FxFG-nucleoporin core revealed that both identical FxFG-binding sites on the NTF2 dimer are formed by residues from each chain; engineered mutations at this interface reduce NPC binding and cause reduced growth and Ran mislocalization in yeast. X-ray crystallography, yeast mutagenesis, growth assay, Ran localization The EMBO journal High 12065398
2002 NTF2 is exported from isolated oocyte nuclei approximately 30 times faster than GFP (a similarly sized inert molecule), demonstrating facilitated NPC translocation dependent on FG-repeat binding. Optical single transporter recording in isolated Xenopus oocyte nuclei and purified nuclear envelopes EMBO reports Medium 12189172
2002 Drosophila NTF2 (DNTF-2) is essential for nuclear import; hypomorphic alleles block nuclear translocation of NF-κB/Rel proteins (Dorsal, Dif, Relish) after infection, impairing antimicrobial peptide gene expression; DNTF-2 directly interacts with Mbo/DNup88, a nucleoporin lacking FG repeats. Drosophila genetics (hypomorphic alleles, lethality), nuclear localization assay for Rel proteins, direct protein interaction EMBO reports Medium 11943764
2004 A novel ATP-dependent activity releases RanGDP from NTF2 (acts as a RanGDF — GDI displacement factor); hydrolyzable ATP enhances GDP dissociation from the Ran–NTF2 complex, and this activity is regulated by phosphorylation (inhibited by a phosphatase inhibitor mixture). In vitro GDP dissociation assay with hydrolyzable vs. non-hydrolyzable ATP analogues, phosphatase inhibitor treatment The Journal of biological chemistry Medium 15155737
2004 A D23A mutation in NTF2 increases affinity for the NPC without gross structural change; residue D23 lies in an evolutionarily conserved region of the NTF2-domain superfamily that functions as an NPC-binding site distinct from the FxFG-binding site. Computational evolutionary analysis, mutagenesis, NPC-binding assay Journal of molecular biology Medium 15522285
2006 Molecular dynamics simulations identified six FG-repeat peptide binding spots on the NTF2 surface forming a stripe, including two novel sites beyond the four known from X-ray/NMR data, providing a model for how FG repeats recognize NTF2 during NPC transit. Molecular dynamics simulation (254+ ns), agreement with prior X-ray, mutational, NMR, and computational data Journal of molecular biology Low 17161424
2010 Nuclear size scales with NTF2 concentration in Xenopus egg extracts: higher NTF2 reduces nuclear size and selectively reduces import of large cargo molecules. NTF2 and importin-α together account for nuclear size differences between X. laevis and X. tropicalis, both acting through lamin B3 import. Xenopus egg extract nuclear reconstitution, titration of NTF2 and importin-α, fluorescent import assays Cell High 20946986
2010 In senescent cells, NTF2 is sequestered in the nucleus alongside reduced RanGTP restoration (due to ATP deficiency), contributing to failure of actin export via exportin 6. GST-pulldown (Exp6-RanGTP interaction), cellular fractionation, Exp6 knockdown, H2O2-induced senescence Experimental cell research Low 21195711
2012 Translocation of NTF2 from nucleus to cytoplasm to collect RanGDP is subject to regulation: treatment with polysorbitan monolaurate blocks NTF2 nuclear export (not import), causing cytoplasmic Ran accumulation and inhibiting RanGTP-dependent nuclear export processes; rescue requires NTF2 overexpression. Increased phosphorylation of tyrosine/threonine proteins accompanies this block. Polysorbitan monolaurate treatment, NTF2 overexpression rescue, tRNA and protein export assays, phosphorylation analysis PloS one Medium 22880006
2016 NTF2 reduces nuclear pore complex diameter in a Ran-binding-dependent manner in Xenopus extracts; Ran binding to NTF2 is required for NTF2 to inhibit nuclear expansion and large-cargo import. Ectopic NTF2 expression reduces nuclear size in Xenopus embryos and mammalian tissue culture cells. Xenopus egg and embryo extract nuclear reconstitution, NTF2 mutants deficient in Ran binding, NPC diameter measurement, ectopic NTF2 expression in mammalian cells Journal of cell science High 26823604
2023 Native top-down proteomics revealed that EGFR signaling induces dissociation of NUTF2 dimers in breast cancer cells; NUTF2 proteoforms with K4 and K55 post-translational modifications differentially impact NUTF2's inhibition of the estrogen receptor (ER) signaling pathway. Native top-down proteomics (SEC, nano-LC, FAIMS, multistage MS), identification of endogenous proteoform complexes Research squarepreprint Low 37546719
2024 SANS with site-specific deuteration showed that FG-Nup repeats remain fully disordered upon binding to NTF2 in vitro, with no significant inter-aromatic contacts or interchain linkage in the FG-Nup–NTF2 complex. Small-angle neutron scattering (SANS) with site-specific deuteration and model fitting bioRxivpreprint Medium

Source papers

Stage 0 corpus · 56 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1998 NTF2 mediates nuclear import of Ran. The EMBO journal 365 9822603
1995 Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. The Journal of cell biology 353 7744965
2010 Nuclear size is regulated by importin α and Ntf2 in Xenopus. Cell 202 20946986
1998 Nuclear import of Ran is mediated by the transport factor NTF2. Current biology : CB 183 9889103
1999 Interaction between NTF2 and xFxFG-containing nucleoporins is required to mediate nuclear import of RanGDP. Journal of molecular biology 154 10543952
2002 Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats. The EMBO journal 143 12065398
1998 Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran. Journal of molecular biology 140 9533885
1996 The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2). Journal of molecular biology 129 8757804
1996 Separate binding sites on nuclear transport factor 2 (NTF2) for GDP-Ran and the phenylalanine-rich repeat regions of nucleoporins p62 and Nsp1p. Journal of molecular biology 111 8918934
1991 Phosphotyrosyl turnover in insulin signaling. Characterization of two membrane-bound pp15 protein tyrosine phosphatases from 3T3-L1 adipocytes. The Journal of biological chemistry 96 1848858
1996 The NTF2 gene encodes an essential, highly conserved protein that functions in nuclear transport in vivo. The Journal of biological chemistry 92 8702493
1988 Effect of vanadate on the cellular accumulation of pp15, an apparent product of insulin receptor tyrosine kinase action. The Journal of biological chemistry 91 2458350
1988 Identification of phosphorylated 422(aP2) protein as pp15, the 15-kilodalton target of the insulin receptor tyrosine kinase in 3T3-L1 adipocytes. Proceedings of the National Academy of Sciences of the United States of America 91 2848242
1996 Nucleotide-specific interaction of Ran/TC4 with nuclear transport factors NTF2 and p97. Proceedings of the National Academy of Sciences of the United States of America 78 8755535
1999 Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export. Molecular and cellular biology 72 10567585
1997 Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran. Journal of molecular biology 65 9368653
1992 Structural and functional characterization of the phosphorylated adipocyte lipid-binding protein (pp15). Biochemistry 61 1372828
1998 The structure of the Q69L mutant of GDP-Ran shows a major conformational change in the switch II loop that accounts for its failure to bind nuclear transport factor 2 (NTF2). Journal of molecular biology 60 9878368
2013 Filling out the structural map of the NTF2-like superfamily. BMC bioinformatics 57 24246060
2006 Association of nuclear pore FG-repeat domains to NTF2 import and export complexes. Journal of molecular biology 57 17161424
2013 Crystal structures of the human G3BP1 NTF2-like domain visualize FxFG Nup repeat specificity. PloS one 56 24324649
1996 Exogenously injected nuclear import factor p10/NTF2 inhibits signal-mediated nuclear import and export of proteins in living cells. FEBS letters 50 8955342
2002 Rapid translocation of NTF2 through the nuclear pore of isolated nuclei and nuclear envelopes. EMBO reports 45 12189172
2015 Crystal structure of the G3BP2 NTF2-like domain in complex with a canonical FGDF motif peptide. Biochemical and biophysical research communications 42 26410532
2022 SARS-CoV-2 Nucleocapsid Protein Targets a Conserved Surface Groove of the NTF2-like Domain of G3BP1. Journal of molecular biology 41 35240128
2016 Nuclear size is sensitive to NTF2 protein levels in a manner dependent on Ran binding. Journal of cell science 38 26823604
2000 Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran. Molecular biology of the cell 37 10679025
1998 Nuclear transport factor p10/NTF2 functions as a Ran-GDP dissociation inhibitor (Ran-GDI). Current biology : CB 32 9843686
2000 The interaction between Ran and NTF2 is required for cell cycle progression. Molecular biology of the cell 27 10930458
2002 The Drosophila homolog of NTF-2, the nuclear transport factor-2, is essential for immune response. EMBO reports 25 11943764
2004 Computational and biochemical identification of a nuclear pore complex binding site on the nuclear transport carrier NTF2. Journal of molecular biology 23 15522285
2015 NTF2-like domain of Tap plays a critical role in cargo mRNA recognition and export. Nucleic acids research 20 25628355
2019 Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds. Scientific reports 19 31792250
2020 Long Noncoding RNA LINC00173 Promotes NUTF2 Expression Through Sponging miR-765 and Facilitates Tumorigenesis in Glioma. Cancer management and research 18 32848473
2011 The structure of the NXF2/NXT1 heterodimeric complex reveals the combined specificity and versatility of the NTF2-like fold. Journal of molecular biology 18 22123199
1999 Engineered mutants in the switch II loop of Ran define the contribution made by key residues to the interaction with nuclear transport factor 2 (NTF2) and the role of this interaction in nuclear protein import. Journal of molecular biology 17 10356329
2012 Crystal structure of the Rasputin NTF2-like domain from Drosophila melanogaster. Biochemical and biophysical research communications 14 22414690
2020 Structural characterization of three noncanonical NTF2-like superfamily proteins: implications for polyketide biosynthesis. Acta crystallographica. Section F, Structural biology communications 12 32744249
2004 An ATP-dependent activity that releases RanGDP from NTF2. The Journal of biological chemistry 11 15155737
1980 [New placental protein (PP15) with immunosuppressive properties (author's transl)]. Archives of gynecology 11 7458421
2010 Reduction of exportin 6 activity leads to actin accumulation via failure of RanGTP restoration and NTF2 sequestration in the nuclei of senescent cells. Experimental cell research 10 21195711
2016 Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027. The Journal of antibiotics 8 27406907
2010 Cloning, expression and characterization of the putative nuclear transport factor 2 (NTF2) gene from moss Conocephalum conicum(L.) Dum. Molecular biology reports 8 20857212
2024 Targeting the NTF2-like domain of G3BP1: Novel modulators of intracellular granule dynamics. Biochemical and biophysical research communications 7 38262290
2012 Nuclear-cytoplasmic trafficking of NTF2, the nuclear import receptor for the RanGTPase, is subjected to regulation. PloS one 7 22880006
2010 Purification, crystallization and preliminary X-ray diffraction of the G3BP1 NTF2-like domain. Acta crystallographica. Section F, Structural biology and crystallization communications 7 21206022
2021 Peptidoglycan binding by a pocket on the accessory NTF2-domain of Pgp2 directs helical cell shape of Campylobacter jejuni. The Journal of biological chemistry 5 33711341
2020 pH and alcohol induced structural transition in Ntf2 a nuclear transport factor of Saccharomyces cerevisiae. International journal of biological macromolecules 5 32407943
2023 Native top-down proteomics reveals EGFR-ERα signaling crosstalk in breast cancer cells dissociates NUTF2 dimers to modulate ERα signaling and cell growth. Research square 3 37546719
2023 Biodegradation of Choline NTF2 by Pantoea agglomerans in Different Osmolarity. Characterization and Environmental Implications of the Produced Exopolysaccharide. Polymers 3 37836024
2007 Phosphate removal under denitrifying conditions by Brachymonas sp. strain P12 and Paracoccus denitrificans PP15. Canadian journal of microbiology 3 17668033
2025 Buttressing ligand-binding pockets in de novo designed NTF2-like domains. Protein science : a publication of the Protein Society 2 41074781
2018 Chemical shift assignments of RHE_RS02845, a NTF2-like domain-containing protein from Rhizobium etli. Biomolecular NMR assignments 1 29572784
2017 UDP and NTF2 are the most consistently expressed genes in Panax ginseng roots at different growth stages. Molecular medicine reports 1 28440415
2026 Exposure of Geobacter-enriched electro-active biofilm to [BMIM][NTf2] ionic liquid inhibits current generation in a bioelectrochemical system. Bioelectrochemistry (Amsterdam, Netherlands) 0 41934702
2025 NUTF2 Promotes Proliferation of Oral Squamous Cell Carcinoma Through PI3K/AKT Signaling Pathway. Oral diseases 0 40544499

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