Affinage

NR2F1

COUP transcription factor 1 · UniProt P10589

Length
423 aa
Mass
46.2 kDa
Annotated
2026-06-10
100 papers in source corpus 44 papers cited in narrative 44 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NR2F1 (COUP-TFI/EAR-3) is an orphan nuclear receptor that functions chiefly as a sequence-specific transcriptional repressor and a graded intrinsic determinant of neural cell-fate decisions (PMID:9171235, PMID:17828260). It binds nuclear receptor response elements—direct repeats, half-sites, and RAREs—often competing with or displacing activating receptors such as HNF-4, RAR:RXR, and Nkx2.5 from shared sites to silence target promoters including ApoB/ApoCIII/ApoAII, Oct-3/4, MTTP, and calreticulin (PMID:1639815, PMID:7823919, PMID:11106640, PMID:22357705). Its repressive output is executed through a C-terminal repressor domain that recruits the corepressors N-CoR and SMRT, and it nucleates a larger silencing complex containing NCoR, HDAC1, TIF1β/KAP-1, Brahma, and DBC1 (PMID:9171235, PMID:19112178); in a context-dependent manner it can also activate transcription via the coactivators GRIP1/SRC-1 and cooperate with ERα, enhancing ERα AF-1 activity through ERK2-mediated Ser118 phosphorylation (PMID:10652338, PMID:12093745). In neural development NR2F1 patterns the neocortex as a graded factor that represses frontal/motor identities and specifies sensory areas, controls neuronal birth timing and the neurogenic-to-gliogenic transition, directs cortical interneuron subtype specification partly by driving Sox6, and promotes radial migration of callosal projection neurons by directly repressing Rnd2 (PMID:17828260, PMID:19160499, PMID:28694260, PMID:21965613). It governs axon growth and commissure formation through MAP1B/RND2, oligodendrocyte differentiation upstream of SCIP/Oct-6, adult hippocampal neurogenesis versus astrogliogenesis, and activity-dependent TH expression in olfactory dopaminergic neurons via ZIF268 (PMID:17021036, PMID:14738874, PMID:29996095, PMID:24227652). NR2F1 dosage is itself tightly controlled epigenetically and post-transcriptionally—by PRC2/H3K27me3, SETD2/H3K36me3, and METTL3-mediated m6A modification (PMID:23666625, PMID:38056895, PMID:35936005). In cancer, NR2F1 is a master node for disseminated tumor cell dormancy, inducing global chromatin repression and SOX9/RARβ/CDK-inhibitor/NANOG programs to enforce quiescence and suppress metastasis, an effect that can be triggered pharmacologically by a specific small-molecule agonist (PMID:25636082, PMID:34812843). Heterozygous NR2F1 loss-of-function mutations cause Bosch-Boonstra-Schaaf optic atrophy syndrome (BBSOAS), with patient mutations in the DNA-binding and ligand-binding domains reducing transcriptional activity and haploinsufficiency producing cortical malformations linked to dysregulated self-renewal and Pax6 (PMID:24462372, PMID:32484994).

Mechanistic history

Synthesis pass · year-by-year structured walk · 19 steps
  1. 1992 High

    Established that NR2F1 is a sequence-specific DNA-binding repressor that silences metabolic gene promoters by competing with activating receptors, defining its core biochemical mode of action.

    Evidence EMSA with quantified affinity, competition binding, and cotransfection reporter assays on ApoB/ApoCIII/ApoAII promoters in HepG2 cells

    PMID:1639815

    Open questions at the time
    • Did not identify the corepressor machinery executing repression
    • Did not test endogenous chromatin occupancy
  2. 1995 High

    Showed NR2F1 represses Oct-3/4 by outcompeting RAR:RXR at a shared RAREoct element and that retinoic acid upregulates NR2F1, linking it to RA-driven differentiation programs.

    Evidence EMSA competition, reporter assays, and C-terminal deletion mutagenesis in P19 embryonal carcinoma cells

    PMID:7823919

    Open questions at the time
    • Mechanism of how RA induces NR2F1 not resolved
    • Corepressor identity at the locus not defined
  3. 1997 High

    Defined the molecular basis of repression by mapping a C-terminal repressor domain that binds the corepressors N-CoR and SMRT, shared with unliganded TRβ.

    Evidence Yeast two-hybrid, GST pull-down, squelching/repression assays, deletion mutagenesis

    PMID:9171235

    Open questions at the time
    • Did not show the full multiprotein complex in cells
    • Did not establish target gene specificity in vivo
  4. 2000 Medium

    Revealed NR2F1's dual activator capacity and its physical/regulatory cross-talk with ERα, AHR, and ERRα1, extending it beyond pure repression.

    Evidence In vitro protein interaction assays, co-IP, EMSA on XRE, reporter assays in CV-1/MCF-7 cells; coactivator (GRIP1/SRC-1) co-IP and domain mapping

    PMID:10620335 PMID:10652338

    Open questions at the time
    • Interaction interfaces not structurally resolved
    • Physiological relevance of AHR competition untested in vivo
  5. 1999 High

    Demonstrated NR2F1 is an intrinsic cortical patterning factor required for subplate neuron differentiation and thalamocortical innervation, beginning the neural developmental program.

    Evidence Coup-tf1 null mouse knockout with histology and axon tracing

    PMID:10624948

    Open questions at the time
    • Direct transcriptional targets in subplate not identified
    • Cell-autonomous vs non-autonomous contributions unresolved at this stage
  6. 2001 High

    Showed NR2F1 acts thalamus-independently as an intrinsic arealization determinant working with Pax6 and Emx2 to specify neocortical identity.

    Evidence COUP-TFI null mouse, region-specific marker analysis, axon tracing

    PMID:11511537

    Open questions at the time
    • Direct downstream area-identity genes not yet mapped
    • Graded dosage mechanism not yet demonstrated
  7. 2007 High

    Established NR2F1 as a graded determinant balancing frontal/motor versus sensory area identity and a regulator of progenitor cell-cycle exit, linking arealization to proliferation control.

    Evidence Cortex-specific conditional knockout with marker and axonal tracing; LOF/GOF with pERK/pAKT/β-catenin readouts

    PMID:17828260 PMID:18165280

    Open questions at the time
    • Signaling repression (#18165280) inferred from expression, not direct biochemistry
    • Direct target genes of arealization repression incompletely defined
  8. 2010 High

    Identified Fabp7 as a directly bound and chromatin-state-controlled NR2F1 target via genome-scale ChIP integration, providing in vivo target validation.

    Evidence Microarray (KO vs WT), computational site prediction, in vivo/in vitro ChIP, chromatin mark analysis, Western blot

    PMID:20111703

    Open questions at the time
    • Genome-wide direct target set still incomplete
    • Single-tissue (inner ear) ChIP limits generality
  9. 2010 High

    Resolved how NR2F1 controls neuronal birth timing and axonal/migration programs, repressing the corticospinal motor neuron program and directly repressing Rnd2 to enable radial migration.

    Evidence Conditional knockouts with axon tracing, behavior, and in utero electroporation Rnd2 rescue

    PMID:17021036 PMID:20133588 PMID:21965613

    Open questions at the time
    • Whether Rnd2/MAP1B are the sole effectors of migration phenotypes unclear
    • Direct binding at the Rnd2 promoter not fully mapped
  10. 2011 High

    Demonstrated NR2F1 specifies cortical interneuron subtype identity (CGE-derived VIP+/CR+ vs MGE-derived PV+), later shown to act partly by directly driving Sox6.

    Evidence Conditional knockouts, subtype marker analysis, pharmacological seizure assays; MGE-progenitor cKO with Sox6 analysis

    PMID:21430164 PMID:28694260

    Open questions at the time
    • Direct vs indirect regulation of Sox6 not biochemically confirmed
    • Redundancy with NR2F2 not fully partitioned
  11. 2008 High

    Defined the in-cell NR2F1 repressor complex (NCoR/HDAC1/KAP-1/Brahma/DBC1) and a function in TNFα signaling via TNFAIP8 repression, and established NR2F1/NR2F2 as a switch for the neurogenic-to-gliogenic transition.

    Evidence Tandem affinity purification and in vitro binding for the complex; double shRNA knockdown in ESC-derived NSPCs and forebrain for the temporal switch

    PMID:19112178 PMID:19160499

    Open questions at the time
    • Stoichiometry and assembly order of the complex unresolved
    • Mechanism coupling the complex to gliogenic competence not defined
  12. 2013 Medium

    Showed NR2F1 dosage is itself epigenetically tuned by PRC2 and embedded in reciprocal cortical regulatory circuits, including with Sp8, and drives an activity-dependent ZIF268→TH axis in olfactory neurons.

    Evidence Suz12 shRNA + ChIP for PRC2; transgenic/conditional Sp8 manipulation; conditional and lentiviral cell-autonomous NR2F1 deletion with odor deprivation

    PMID:23307639 PMID:23666625 PMID:24227652

    Open questions at the time
    • Whether PRC2 acts directly or indirectly on NR2F1 in vivo unresolved
    • ZIF268 as the sole intermediary to TH not excluded
  13. 2014 Medium

    Linked NR2F1 to human disease (BBSOAS) by demonstrating patient missense mutations in the DNA-binding and ligand-binding domains reduce transcriptional activity.

    Evidence Luciferase reporter assays with patient-derived mutant constructs

    PMID:24462372

    Open questions at the time
    • Cell-based assay does not capture neural-specific consequences
    • Dominant-negative vs haploinsufficiency mechanism not distinguished here
  14. 2015 High

    Identified NR2F1 as a master inducer of disseminated tumor cell dormancy, enforcing quiescence through global chromatin repression and a SOX9/RARβ/CDK-inhibitor/NANOG program.

    Evidence In vitro/in vivo dormancy models, 5-Aza-C+RA co-treatment, knockdown/overexpression, ChIP, patient bone marrow DTC analysis

    PMID:25636082

    Open questions at the time
    • Direct genomic targets driving chromatin repression incompletely mapped
    • Signals upstream that activate NR2F1 in DTCs not defined here
  15. 2018 High

    Established NR2F1 as necessary and sufficient to bias adult hippocampal NSCs toward neurogenesis over astrogliogenesis, including rescue under neuroinflammation.

    Evidence Inducible cKO, retroviral LOF, fate mapping, GOF overexpression, neuroinflammation model

    PMID:29996095

    Open questions at the time
    • Transcriptional targets controlling the neuro/astroglial switch not identified
    • Mechanism of neuroinflammation rescue unresolved
  16. 2019 High

    Resolved how NR2F1 expression boundaries are set by the Egr→Foxg1 enhancer circuit and how NR2F1 controls hippocampal neurogenesis/migration via Cxcr4, plus its broader role in hippocampal structure and spatial memory.

    Evidence ChIP at H3K4me1 enhancer, in utero electroporation, conditional knockouts; progenitor cKO with Cxcr4 GOF phenocopy; cortical cKO with volumetry, tracing, and behavior

    PMID:26813976 PMID:28506990 PMID:31395862

    Open questions at the time
    • Direct NR2F1 binding at Cxcr4 not shown
    • Behavioral deficits not mechanistically tied to a single target
  17. 2020 Medium

    Connected NR2F1 dosage to BBSOAS pathology by showing haploinsufficiency disrupts neural progenitor self-renewal and Pax6, and to breast cancer dissemination through p38α/HER2/WNT4 control and EMT suppression.

    Evidence Haploinsufficient mouse, patient MRI, cerebral organoids; intravital imaging, LOF, pathway inhibitors, EMT/E-cadherin/β-catenin analysis

    PMID:32484994 PMID:35471456

    Open questions at the time
    • Direct vs indirect regulation of Pax6 unresolved
    • Upstream signaling links to NR2F1 (#35471456) rely on inhibitor/overexpression in single lab
  18. 2021 High

    Demonstrated NR2F1 is pharmacologically actionable—a small-molecule agonist self-amplifies NR2F1 to enforce dormancy, inhibit mTOR, and suppress metastasis—and that NR2F1 restrains dopaminergic differentiation timing relevant to Parkinson's disease.

    Evidence Agonist treatment with CRISPR-Cas9 KO validation, RNA-seq, organoids, in vivo metastasis; LRRK2-G2019S iPSC NESCs, scRNA-seq, NR2F1-deficient mouse embryos, midbrain organoids

    PMID:34686322 PMID:34812843

    Open questions at the time
    • Direct molecular target/binding mode of the agonist not defined
    • How LRRK2-G2019S lowers NR2F1 mechanistically unresolved
  19. 2023 Medium

    Showed multilayered control of NR2F1 mRNA/chromatin (METTL3 m6A, SETD2/H3K36me3) couples NR2F1 dosage to epithelial integrity, inflammation, and STAT1-driven immune signaling.

    Evidence MeRIP-seq and rescue in RPE cells; ATAC-seq/ChIP-seq/RNA-seq with Setd2-KO syngeneic mouse models

    PMID:35936005 PMID:38056895

    Open questions at the time
    • Which m6A reader transduces the METTL3 effect not identified
    • Direct NR2F1 targets in STAT1 axis not fully mapped

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NR2F1's ligand-binding domain is regulated (true ligand vs constitutive activity) and how it switches between repressor and activator modes across cell contexts remains unresolved.
  • No endogenous ligand defined despite agonist responsiveness
  • Determinants of corepressor vs coactivator recruitment context not established
  • Genome-wide direct target maps across neural and tumor states incomplete

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 6 GO:0003677 DNA binding 5 GO:0098772 molecular function regulator activity 2
Localization
GO:0005634 nucleus 3
Pathway
R-HSA-1266738 Developmental Biology 6 R-HSA-1643685 Disease 4 R-HSA-74160 Gene expression (Transcription) 4 R-HSA-4839726 Chromatin organization 3
Partners
AHRCCAR2ESR1HDAC1NCOA2NCOR1NCOR2TRIM28
Complex memberships
NCoR/HDAC1/KAP-1/Brahma/DBC1 corepressor complex

Evidence

Reading pass · 44 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 COUP-TFI (NR2F1) represses transcription via direct interaction with corepressors N-CoR and SMRT. The C-terminal 35 amino acids of COUP-TFI constitute a repressor domain required for silencing activity. COUP-TFI and unliganded TRβ share common corepressors, demonstrated by squelching experiments and yeast two-hybrid/GST pull-down assays showing direct binding to N-CoR (aa 921-2453) and SMRT fragments. Yeast two-hybrid, GST pull-down, cotransfection repression/squelching assays, deletion mutagenesis Molecular endocrinology High 9171235
1992 EAR-3/COUP-TFI binds specifically to regulatory elements BA1, CIIIB, and AIIJ in the ApoB, ApoCIII, and ApoAII gene promoters with Kd 1–3 nM, and represses transcription from these elements in cotransfection experiments in HepG2 cells. EAR-3 can compete with and displace HNF-4 from the same elements to suppress transcription. EMSA (dissociation constant measurement), cotransfection reporter assays in HepG2 cells, competition binding assays The Journal of biological chemistry High 1639815
1995 EAR-3/COUP-TFI represses Oct-3/4 promoter activity through the RAREoct site in P19 embryonal carcinoma cells in a dose-dependent manner via the C-terminal domain. COUP-TFI binds RAREoct with higher affinity than RAR:RXR heterodimers, allowing it to compete with and displace RAR:RXR from the site, actively silencing Oct-3/4 promoter activity. RA treatment of EC cells upregulates COUP-TFI expression, which inversely correlates with Oct-3/4 repression. Cotransfection reporter assays, EMSA competition/binding assays, deletion mutagenesis of C-terminal domain Molecular and cellular biology High 7823919
1999 Loss of COUP-TFI in mouse cortex results in absence of cortical layer IV due to failure of thalamocortical projections, and subplate neurons undergo improper differentiation and premature cell death. COUP-TFI is required for subplate neuron differentiation which in turn guides thalamocortical axon innervation. Coup-tf1 null mouse knockout, histology, axon tracing Neuron High 10624948
2001 COUP-TFI acts as an intrinsic factor for early regionalization of the neocortex, independent of thalamic input. COUP-TFI null mice show altered region-specific expression of cortical marker genes and miswired area-specific thalamocortical connections, indicating COUP-TFI works in concert with Pax6 and Emx2 to specify neocortical identity. COUP-TFI null mouse knockout, marker gene expression analysis, axon tracing Genes & development High 11511537
2007 Cortex-specific deletion of COUP-TFI in mice causes massive expansion of frontal/motor areas and compression of sensory areas. COUP-TFI acts within its expression domain (highest in parietal/occipital, lowest in frontal cortex) to repress frontal/motor area identities and specify sensory area identities, demonstrating it is required for balancing neocortical arealization. Cortex-specific conditional knockout (Cre-lox), molecular marker analysis, axonal projection tracing Nature neuroscience High 17828260
2007 COUP-TFI loss-of-function and gain-of-function manipulations show it promotes ventral cortical fate, promotes cell cycle exit and neural differentiation, and regulates the balance of early- and late-born neurons, in part by repressing MAPK/ERK, AKT, and β-catenin signaling in cortical progenitors. Loss-of-function and gain-of-function in vivo and in vitro, signaling pathway readouts (pERK, pAKT, β-catenin) Cerebral cortex Medium 18165280
2008 COUP-TFI (and COUP-TFII) are required for temporal specification of neural stem/progenitor cells in the CNS, including acquisition of gliogenic competence. Double knockdown of Coup-tfI/II in ESC-derived NSPCs and developing mouse forebrain causes sustained neurogenesis and prolonged generation of early-born neurons, demonstrating these factors function as molecular switches for the neurogenic-to-gliogenic transition. Double shRNA knockdown in ESC-derived NSPCs and in vivo mouse forebrain, cell fate marker analysis, gliogenic cytokine responsiveness assays Nature neuroscience High 19160499
2008 COUP-TFI forms a transcriptional repressor complex with NCoR, TIF1β/KAP-1, HDAC1, SWI/SNF member Brahma, and DBC1 in HeLa cells. Tandem affinity purification identified these components. COUP-TFI interacts directly with NCoR in a manner different from other nuclear receptors; DBC1 stabilizes the COUP-TFI–NCoR interaction. This complex represses TNFAIP8 transcription and is involved in TNFα signaling. Tandem affinity purification (TAP), in vitro binding assays, cotransfection reporter assays, TNFAIP8 promoter analysis The Journal of biological chemistry High 19112178
2006 COUP-TFI is required for axonal growth and guidance of forebrain commissures (corpus callosum, hippocampal commissure, anterior commissure). In COUP-TFI null mice, fibers fail to cross the midline. Microarray analysis of COUP-TFI null cortex reveals decreased MAP1B and increased RND2 protein levels, identifying these cytoskeleton regulators as downstream effectors of COUP-TFI in axonal morphogenesis. COUP-TFI null mouse knockout, axon tracing, microarray expression profiling, primary hippocampal neuron culture, Western blot Development High 17021036
2010 COUP-TFI controls timing of birth and specification of corticospinal motor neurons (CSMN) in somatosensory cortex by repressing a CSMN differentiation program. Loss of COUP-TFI causes premature area-specific generation of neurons with CSMN features that project to subcerebral structures, while genuine CSMN fail to project beyond the pons, resulting in impaired skilled motor function. Conditional cortical COUP-TFI knockout, axon tracing, motor behavior assays, molecular marker analysis PNAS High 20133588
2011 COUP-TFI is required for specification of CGE-derived cortical interneurons. Conditional loss of COUP-TFI in subventricular precursors and postmitotic cells leads to decreased late-born VIP+ and CR+ bipolar neurons and compensatory increase of early-born PV+ MGE-derived interneurons. COUP-TFI mutants are more resistant to pharmacologically induced seizures in a GABAergic signaling-dependent manner. Conditional knockout (subventricular precursor and postmitotic), interneuron subtype marker analysis, pharmacological seizure induction The Journal of neuroscience High 21430164
2011 COUP-TFI directly represses Rnd2 (a Rho-GTPase modulator of radial migration) expression at the post-mitotic level along the rostrocaudal axis of the neocortex. In COUP-TFI mutant mice, callosal projection neurons are delayed in reaching the cortical plate and have morphological defects. Restoring correct Rnd2 levels cell-autonomously rescues neuron radial migration and morphological transitions as well as axonal elongation and dendritic arborization defects. COUP-TFI conditional knockout, in utero electroporation rescue (Rnd2 shRNA), immunostaining, axon/dendrite morphology analysis Development High 21965613
2000 COUP-TFI interacts directly with ERα (estrogen receptor alpha), COUP-TF, and ERRα1 in a ligand-dependent manner in vitro. AHR interacts with COUP-TFI in transfected CV-1 cells. COUP-TFI binds consensus xenobiotic response elements (XRE), and overexpression of COUP-TFI inhibits TCDD-activated CYP1A1 promoter reporter activity, suggesting COUP-TFI regulates AHR action via DNA binding competition and protein-protein interaction. In vitro protein-protein interaction assays, transfection in CV-1 cells, EMSA (XRE binding), reporter assay in MCF-7 cells Archives of biochemistry and biophysics Medium 10620335
2002 COUP-TFI forms a tight physical complex with human ERα. This complex increases the affinity of ERα for ERK2/p42MAPK, resulting in enhanced phosphorylation of hERα Ser118 and strengthened AF-1 transactivation activity. The cooperation is restricted to cell lines permissive to ERα AF-1 activation. Co-immunoprecipitation (complex formation), kinase assay (Ser118 phosphorylation), transfection in multiple cell lines, functional reporter assays The EMBO journal High 12093745
2003 EAR3/COUP-TFI represses LHR gene transcription via a mechanism involving cross-talk among EAR3, Sp1/Sp3 bound at the Sp1(I) site, and TFIIB. EAR3 specifically decreases association of TFIIB with the Sp1(I) site and reduces RNA polymerase II recruitment to the LHR promoter. Overexpression of TFIIB counteracts EAR3-mediated inhibition. EAR3's N-terminal and DNA-binding domains are required for Sp1 interaction and inhibitory function. Cotransfection reporter assays (SL2 cells), ChIP (RNA pol II recruitment), EMSA/co-IP (TFIIB-Sp1 interaction), deletion mutagenesis Molecular and cellular biology High 12972613
2003 COUP-TF1 represses HBV pregenomic RNA and pre-C RNA synthesis >10-fold through nuclear receptor response elements NRREpreC and NRREenhI. HNF4α and COUP-TF1 antagonize each other's effects on HBV gene expression and viral DNA synthesis. A naturally occurring HBV variant that prevents COUP-TF1 binding but retains HNF4α binding shows significantly higher pregenomic RNA and viral DNA synthesis. Cotransfection in Huh7 cells, viral RNA quantification, viral DNA synthesis assay, natural variant analysis, deletion analysis Journal of virology Medium 12551987
2004 COUP-TFI is expressed in oligodendrocyte lineage cells and is required for oligodendrocyte differentiation and CNS myelination. COUP-TFI null mice show delayed axon myelination and increased dysmyelination. In vitro differentiation assays confirm myelination defects are due to delayed oligodendrocyte differentiation. COUP-TFI acts as an upstream regulator of SCIP/Oct-6/Tst-1, a transcription factor involved in axon myelination. COUP-TFI null mouse knockout, in vitro oligodendrocyte differentiation assay, in situ hybridization, transfection analysis Developmental biology High 14738874
2000 COUP-TFI inhibits BMP-4 1A promoter activity in fetal rat calvarial osteoblasts. Specific binding of COUP-TFI to response regions in the BMP-4 1A 5'-flanking region was demonstrated, and co-transfection of a COUP-TFI expression plasmid with the BMP-4 1A promoter construct showed COUP-TFI silences BMP-4 transcription. EMSA (specific binding to promoter elements), cotransfection reporter assay in primary osteoblasts The Journal of biological chemistry Medium 7499338
2000 COUP-TFI binds the Nkx2.5 binding site in the calreticulin gene promoter and suppresses calreticulin transcription during cardiac development. Nkx2.5 activates calreticulin gene expression while COUP-TF1 binding to the same site antagonizes Nkx2.5-mediated activation. Cotransfection reporter assays, DNA binding assays, promoter analysis The Journal of biological chemistry Medium 11106640
2000 In differentiating P19 cells, COUP-TFI dose-dependently regulates neuronal differentiation and migration. Low COUP-TFI enhances neuron outgrowth; high COUP-TFI impedes neuronal differentiation and elevates E-cadherin mRNA. Dominant-negative COUP-TFI inhibits neurite extension. COUP-TFI favors neuron migration through an integrin-dependent mechanism and upregulates vitronectin mRNA and promoter activity. Stable and transient transfection in P19 cells, dominant-negative approach, RGD peptide interference assay, RT-PCR, transient transfection reporter assay (vitronectin promoter) Molecular endocrinology Medium 11117523
2000 COUP-TFI (as a transactivator) requires amino acids 184-423 for all three mechanisms of transcriptional activation: binding to gAF1/gAF3 accessory elements, direct DNA binding transactivation, and coactivation through DNA-bound HNF-4. GRIP1 and SRC-1 potentiate COUP-TFI activity and associate with COUP-TFI in vivo via this same region. Deletion mutagenesis, cotransfection reporter assays, in vivo co-immunoprecipitation with GRIP1/SRC-1 The Journal of biological chemistry Medium 10652338
2006 COUP-TFI controls Notch signaling in cochlear development. COUP-TFI knockout mice show increased hair cell number and misregulation of Notch components including Jag1 and Hes5, consistent with reduced Notch signaling. Inhibition of Notch activity by γ-secretase inhibitor causes greater increase in hair cell differentiation in COUP-TFI(-/-) than wild-type cochlear cultures, indicating hypersensitivity to Notch inactivation in COUP-TFI null cochlea. COUP-TFI knockout mouse, in vitro organ culture with γ-secretase inhibitor, Notch target gene expression analysis Development Medium 16914494
2010 Genome-wide identification of direct COUP-TFI target genes using integration of microarray data and COUP-TFI ChIP. Fabp7 is a direct COUP-TFI target: COUP-TFI occupies conserved binding sites in the Fabp7 promoter in vivo (ChIP), Fabp7 transcript and protein are reduced in COUP-TFI null tissues, and the Fabp7 promoter shows active chromatin marks (H3K9ac, CBP, SRC-1 enrichment) dependent on COUP-TFI. Microarray (COUP-TFI KO vs WT inner ear), computational binding site prediction, ChIP (in vivo and in vitro), Western blot, chromatin state analysis PloS one High 20111703
2012 NR2F1 represses MTTP gene expression by binding to a direct repeat 1 (DR1) element in the MTTP promoter, which suppresses synergistic activation by HNF-4α and HNF-1α. This repression is further enhanced by nuclear receptor corepressor 1 (NCoR1). NR2F1 does not alter individual activities of HNF-4α or HNF-1 but specifically abrogates their synergistic cooperation. Cotransfection reporter assays, EMSA (DR1 binding), corepressor overexpression Journal of lipid research Medium 22357705
2013 PRC2 (specifically Suz12-dependent) is recruited to the NR2F1 promoter upon retinoic acid treatment, transiently increasing H3K27me3 marks and attenuating RA-induced NR2F1 transcription. Functional depletion of Suz12 by shRNA enhances RA-associated NR2F1 transcription, identifying PRC2 as a repressive modulator of NR2F1 expression. shRNA depletion of Suz12, RT-PCR, ChIP (H3K27me3, PRC2 occupancy), comparison with Hoxa5 gene Nucleic acids research Medium 23666625
2013 COUP-TFI and Sp8 reciprocally repress each other's expression in cortical progenitors. Misexpression of Sp8 represses COUP-TFI, and conditional ablation of Sp8 has the opposite effect. Transgenic misexpression of COUP-TFI downregulates Sp8. Sp8 promotes FGF target molecule expression in an Fgf8-independent manner. Binary transgenic misexpression, conditional Sp8 knockout, molecular marker analysis in developing cortex Cerebral cortex Medium 23307639
2013 COUP-TFI regulates activity-dependent tyrosine hydroxylase (TH) expression in dopaminergic olfactory bulb interneurons. Odor deprivation downregulates both TH and COUP-TFI in dopaminergic cells. Conditional inactivation of COUP-TFI in the EMX1 lineage reduces TH and ZIF268 expression. Lentiviral COUP-TFI deletion in adult-generated interneurons confirms cell-autonomous control of TH expression through ZIF268 induction. Conditional knockout (EMX1-Cre), odor deprivation paradigm, lentiviral cell-autonomous deletion, immunostaining Development High 24227652
2014 NR2F1 missense mutations in the zinc-finger DNA-binding domain and the putative ligand-binding domain decrease NR2F1 transcriptional activity as measured by luciferase reporter assay, establishing that NR2F1 DNA-binding and ligand-binding domain integrity is required for transcriptional function. Luciferase reporter assay with patient-derived missense mutant constructs American journal of human genetics Medium 24462372
2015 NR2F1 drives tumor cell dormancy by inducing global chromatin repression and upregulating SOX9, RARβ, CDK inhibitors, and the pluripotency gene NANOG. NR2F1-dependent quiescence is recapitulated by co-treatment with 5-Aza-C and retinoic acid. In vivo blockade of NR2F1 interrupts growth arrest or survival of dormant DTCs in different organs. In vitro dormancy models (HNSCC), in vivo mouse models, epigenetic co-treatment (5-Aza-C + RA), gene knockdown/overexpression, ChIP (chromatin repression analysis), bone marrow DTC analysis from prostate cancer patients Nature communications High 25636082
2017 Coup-TF1 and Coup-TF2 (Nr2f1 and Nr2f2) expressed in an arc-shaped MGE progenitor domain promote time-dependent survival of this neuroepithelium and specification of layer V SST+ cortical interneurons. They autonomously repress PV+ fate in MGE progenitors, in part by directly driving Sox6 expression. Conditional knockout of Coup-TF1/TF2 in MGE progenitors, interneuron subtype marker analysis, Sox6 expression analysis Development High 28694260
2019 In the developing sensory neocortex, Foxg1 binds to an H3K4me1-enriched enhancer site to repress COUP-TFI expression. Ectopic Foxg1 in layer 4 cells transforms local projection neurons to callosal projection neurons; removal of Foxg1 in long-range projection neurons derepresses COUP-TFI and activates a layer 4 neuron-specific program. Early growth response genes (Egr) repress Foxg1 in layer 4 precursors via TGF-β signaling. ChIP (H3K4me1 enhancer), in utero electroporation (gain/loss of function), conditional knockout, layer-specific marker analysis Nature communications High 31395862
2018 COUP-TFI/NR2F1 is necessary and sufficient to promote neurogenesis by suppressing astrogliogenesis in adult hippocampal neural stem cells. Inducible COUP-TFI knockout reduces neurogenesis and increases astrocyte production without depleting the NSC pool. Forced COUP-TFI expression decreases astrogliogenesis and rescues the neuro-astrogliogenic imbalance under neuroinflammation. Inducible conditional knockout, retroviral loss-of-function, genetic fate mapping, gain-of-function overexpression, neuroinflammation model Cell reports High 29996095
2019 NR2F1 promotes CXCL12 and CXCR4 expression in salivary adenoid cystic carcinoma cells and activates CXCL12/CXCR4 pathway. ChIP assays demonstrate direct NR2F1 binding at the CXCL12/CXCR4 loci. Overexpression of CXCL12 partially rescues proliferation, migration, and invasion activities suppressed by NR2F1 silencing. NR2F1 overexpression/siRNA knockdown, ChIP (CXCL12/CXCR4 promoters), proliferation/migration/invasion assays, rescue experiments BMC cancer Medium 31357956
2020 NR2F1 regionally controls long-term self-renewal of neural progenitor cells via modulation of cell cycle genes and Pax6. In BBSOAS patients with NR2F1 haploinsufficiency, polymicrogyria-like brain malformations occur in parietal/occipital cortex. In cerebral organoids, reduced NR2F1 levels affect neurogenesis and PAX6 expression. BBSOAS mouse model (Nr2f1 haploinsufficient), patient brain MRI, cerebral organoids (NR2F1 knockdown), cell cycle gene expression analysis The EMBO journal High 32484994
2020 NR2F1 expression is positively regulated by p38α signaling and repressed by HER2 and WNT4 pathways in early-stage breast cancer cells. Loss of NR2F1 function increases in vivo dissemination accompanied by decreased E-cadherin, activated WNT-dependent β-catenin signaling, disorganized laminin 5, and increased EMT genes (TWIST1, ZEB1, PRRX1), promoting a hybrid luminal/basal phenotype. High-resolution intravital imaging, NR2F1 loss-of-function, E-cadherin/β-catenin/laminin immunostaining, signaling pathway inhibitors (p38α, HER2, WNT4), gene expression analysis Cancer research Medium 35471456
2021 A specific small-molecule agonist (DIM-C-Pyr-4 derivative) of NR2F1 activates dormancy programs in malignant cells via a self-regulated increase in NR2F1 mRNA/protein and downstream transcription. This leads to growth arrest, inhibition of mTOR signaling, metastasis suppression, and induction of a neural crest lineage program. The effect is lost when NR2F1 is knocked out by CRISPR-Cas9. Small-molecule agonist treatment, CRISPR-Cas9 NR2F1 knockout (loss of function confirmation), RNA-seq, 3D culture assay, patient-derived organoids, in vivo mouse metastasis model, immunofluorescence (NR2F1hi/p27hi/Ki67lo phenotype) The Journal of experimental medicine High 34812843
2021 LRRK2-G2019S mutation in Parkinson's disease decreases NR2F1 expression in neuroepithelial stem cells, neurons, and midbrain organoids. NR2F1-deficient mouse embryos show accelerated dopaminergic differentiation in vivo, suggesting NR2F1 normally restrains cell-cycle exit and differentiation timing in dopaminergic neurogenesis. Patient-specific NESCs (iPSC-derived), high-throughput image analysis, single-cell RNA sequencing, NR2F1-deficient mouse embryos, midbrain organoids Cell reports Medium 34686322
2022 METTL3 methylates NR2F1 mRNA in an m6A-dependent manner to regulate NR2F1 expression in retinal pigment epithelium cells. NR2F1 deficiency reverses the decreased Occludin expression and increased IL-6 secretion observed in METTL3-defective RPE cells, placing NR2F1 downstream of m6A modification by METTL3 in RPE inflammation regulation. MeRIP-sequencing (m6A mapping), RNA-seq, METTL3 silencing/NR2F1 silencing, Western blot (Occludin), ELISA (IL-6), rescue experiments Frontiers in immunology Medium 35936005
2023 SETD2 inactivation reduces NR2F1 transcription by impairing H3K36me3 deposition and chromatin accessibility at the NR2F1 locus. Reduced NR2F1 activates the STAT1 signaling pathway to promote chemokines, PD-1 expression, and antigen presentation. ChIP-seq and ATAC-seq confirm the SETD2→H3K36me3→NR2F1 chromatin regulatory axis. ATAC-seq, ChIP-seq (H3K36me3), RNA-seq, single-cell RNA-seq, Setd2 knockout syngeneic mouse models, flow cytometry Journal for immunotherapy of cancer High 38056895
2007 COUP-TFI activation of the vitronectin gene in P19 cells is accompanied by recruitment of thymine DNA glycosylase (TDG) and p68 RNA helicase. DNA methyltransferases Dnmt3a and Dnmt3b are loaded onto the activated vitronectin gene, with strand-biased decrease in CpG methylation. Dnmt3a co-immunoprecipitates with COUP-TFI and TDG in vivo; cotransfection shows Dnmt3a/b enhances COUP-TFI-mediated activation of a methylated reporter gene. ChIP (TDG, p68, Dnmt3a occupancy), bisulfite sequencing (CpG methylation), co-immunoprecipitation (Dnmt3a–COUP-TFI–TDG), cotransfection reporter assay Molecular endocrinology Medium 17579209
2009 COUP-TFI binds retinoic acid-responsive elements (RAREs) in the ceramide kinase (CERK) promoter and represses CERK transcription during ATRA-induced differentiation of SH-SY5Y neuroblastoma cells. EMSA shows ATRA-induced binding of COUP-TFI, RARα, and RXRα to the RARE. ChIP confirms recruitment of COUP-TFI and corepressors to this region. siRNA knockdown of COUP-TFI relieves CERK repression. EMSA, DNA pull-down, ChIP (COUP-TFI and corepressor recruitment), siRNA knockdown, promoter truncation/mutation assays Journal of neurochemistry Medium 19903244
2017 COUP-TFI is required for proper hippocampal development along the septo-temporal longitudinal axis. Loss of cortical COUP-TFI results in dysmorphic hippocampus with altered shape, volume, and connectivity, particularly in dorsal and intermediate regions. Topographic entorhinal cortex inputs are strongly impaired in the dorsal hippocampus. These structural changes are associated with selective spatial learning and memory impairment. Cortical COUP-TFI conditional knockout, hippocampal volumetry/shape analysis, axon tracing, spatial memory behavioral testing (Morris water maze or equivalent) Cerebral cortex High 26813976
2019 COUP-TFI is required in dentate gyrus progenitors for modulating Cxcr4 receptor expression during granule cell neurogenesis and migration. Loss of COUP-TFI in progenitors leads to decreased granule cell proliferation, precocious differentiation, increased apoptosis, and high Cxcr4 expression with aberrant migration. High COUP-TFI expression downregulates Cxcr4. Increased Cxcr4 expression in wild-type cells phenocopies COUP-TFI loss-of-function migration defects. Conditional knockout (progenitor vs. postmitotic), gain-of-function Cxcr4 overexpression, immunostaining, proliferation and migration analysis Development High 28506990

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2015 NR2F1 controls tumour cell dormancy via SOX9- and RARβ-driven quiescence programmes. Nature communications 299 25636082
1992 Transcriptional regulation of human apolipoprotein genes ApoB, ApoCIII, and ApoAII by members of the steroid hormone receptor superfamily HNF-4, ARP-1, EAR-2, and EAR-3. The Journal of biological chemistry 285 1639815
2008 Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development. Nature neuroscience 189 19160499
2007 COUP-TFI regulates the balance of cortical patterning between frontal/motor and sensory areas. Nature neuroscience 188 17828260
1999 The nuclear orphan receptor COUP-TFI is required for differentiation of subplate neurons and guidance of thalamocortical axons. Neuron 156 10624948
1997 Gene silencing by chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI) is mediated by transcriptional corepressors, nuclear receptor-corepressor (N-CoR) and silencing mediator for retinoic acid receptor and thyroid hormone receptor (SMRT). Molecular endocrinology (Baltimore, Md.) 152 9171235
2001 COUP-TFI: an intrinsic factor for early regionalization of the neocortex. Genes & development 144 11511537
2018 LncRNA NR2F1-AS1 regulates hepatocellular carcinoma oxaliplatin resistance by targeting ABCC1 via miR-363. Journal of cellular and molecular medicine 138 29602203
1995 A dynamic balance between ARP-1/COUP-TFII, EAR-3/COUP-TFI, and retinoic acid receptor:retinoid X receptor heterodimers regulates Oct-3/4 expression in embryonal carcinoma cells. Molecular and cellular biology 138 7823919
2007 COUP-TFI coordinates cortical patterning, neurogenesis, and laminar fate and modulates MAPK/ERK, AKT, and beta-catenin signaling. Cerebral cortex (New York, N.Y. : 1991) 118 18165280
2014 NR2F1 mutations cause optic atrophy with intellectual disability. American journal of human genetics 112 24462372
2000 The aryl hydrocarbon receptor interacts with estrogen receptor alpha and orphan receptors COUP-TFI and ERRalpha1. Archives of biochemistry and biophysics 102 10620335
2000 Differential expression of COUP-TFI, CHL1, and two novel genes in developing neocortex identified by differential display PCR. The Journal of neuroscience : the official journal of the Society for Neuroscience 101 11027229
2010 Area-specific temporal control of corticospinal motor neuron differentiation by COUP-TFI. Proceedings of the National Academy of Sciences of the United States of America 96 20133588
2018 NR2F1 stratifies dormant disseminated tumor cells in breast cancer patients. Breast cancer research : BCR 93 30322396
1995 The mouse bone morphogenetic protein-4 gene. Analysis of promoter utilization in fetal rat calvarial osteoblasts and regulation by COUP-TFI orphan receptor. The Journal of biological chemistry 92 7499338
2021 Long non-coding RNA NR2F1-AS1 induces breast cancer lung metastatic dormancy by regulating NR2F1 and ΔNp63. Nature communications 88 34475402
2011 Loss of COUP-TFI alters the balance between caudal ganglionic eminence- and medial ganglionic eminence-derived cortical interneurons and results in resistance to epilepsy. The Journal of neuroscience : the official journal of the Society for Neuroscience 87 21430164
2021 An NR2F1-specific agonist suppresses metastasis by inducing cancer cell dormancy. The Journal of experimental medicine 86 34812843
2020 lncRNA NR2F1-AS1 promotes breast cancer angiogenesis through activating IGF-1/IGF-1R/ERK pathway. Journal of cellular and molecular medicine 84 32548873
2006 COUP-TFI is required for the formation of commissural projections in the forebrain by regulating axonal growth. Development (Cambridge, England) 81 17021036
2019 The novel lncRNA lnc-NR2F1 is pro-neurogenic and mutated in human neurodevelopmental disorders. eLife 65 30628890
2022 The EMT-induced lncRNA NR2F1-AS1 positively modulates NR2F1 expression and drives gastric cancer via miR-29a-3p/VAMP7 axis. Cell death & disease 64 35082283
2003 Distinct modes of regulation of transcription of hepatitis B virus by the nuclear receptors HNF4alpha and COUP-TF1. Journal of virology 64 12551987
2011 COUP-TFI promotes radial migration and proper morphology of callosal projection neurons by repressing Rnd2 expression. Development (Cambridge, England) 56 21965613
2004 The nuclear orphan receptor COUP-TFI is important for differentiation of oligodendrocytes. Developmental biology 54 14738874
2019 Long non-coding RNA NR2F1-AS1 promoted proliferation and migration yet suppressed apoptosis of thyroid cancer cells through regulating miRNA-338-3p/CCND1 axis. Journal of cellular and molecular medicine 53 31304680
2020 NR2F1 regulates regional progenitor dynamics in the mouse neocortex and cortical gyrification in BBSOAS patients. The EMBO journal 52 32484994
2017 The Transcription Factors COUP-TFI and COUP-TFII have Distinct Roles in Arealisation and GABAergic Interneuron Specification in the Early Human Fetal Telencephalon. Cerebral cortex (New York, N.Y. : 1991) 51 28922831
2017 Coup-TF1 and Coup-TF2 control subtype and laminar identity of MGE-derived neocortical interneurons. Development (Cambridge, England) 50 28694260
2018 The pleiotropic transcriptional regulator COUP-TFI plays multiple roles in neural development and disease. Brain research 49 29709504
2018 Neuron-Astroglia Cell Fate Decision in the Adult Mouse Hippocampal Neurogenic Niche Is Cell-Intrinsically Controlled by COUP-TFI In Vivo. Cell reports 48 29996095
2019 NR2F1 contributes to cancer cell dormancy, invasion and metastasis of salivary adenoid cystic carcinoma by activating CXCL12/CXCR4 pathway. BMC cancer 45 31357956
2019 LncRNA NR2F1-AS1 is involved in the progression of endometrial cancer by sponging miR-363 to target SOX4. Die Pharmazie 44 31109400
2020 Imbalance of Excitatory/Inhibitory Neuron Differentiation in Neurodevelopmental Disorders with an NR2F1 Point Mutation. Cell reports 43 32320667
2000 COUP-TF1 antagonizes Nkx2.5-mediated activation of the calreticulin gene during cardiac development. The Journal of biological chemistry 42 11106640
2006 COUP-TFI controls Notch regulation of hair cell and support cell differentiation. Development (Cambridge, England) 41 16914494
2013 Sp8 and COUP-TF1 reciprocally regulate patterning and Fgf signaling in cortical progenitors. Cerebral cortex (New York, N.Y. : 1991) 40 23307639
2013 Polycomb recruitment attenuates retinoic acid-induced transcription of the bivalent NR2F1 gene. Nucleic acids research 40 23666625
1999 Synergism between a half-site and an imperfect estrogen-responsive element, and cooperation with COUP-TFI are required for estrogen receptor (ER) to achieve a maximal estrogen-stimulation of rainbow trout ER gene. European journal of biochemistry 40 9914518
2008 A chicken ovalbumin upstream promoter transcription factor I (COUP-TFI) complex represses expression of the gene encoding tumor necrosis factor alpha-induced protein 8 (TNFAIP8). The Journal of biological chemistry 39 19112178
2017 Novel NR2F1 variants likely disrupt DNA binding: molecular modeling in two cases, review of published cases, genotype-phenotype correlation, and phenotypic expansion of the Bosch-Boonstra-Schaaf optic atrophy syndrome. Cold Spring Harbor molecular case studies 38 28963436
2009 NR2F1 deletion in a patient with a de novo paracentric inversion, inv(5)(q15q33.2), and syndromic deafness. American journal of medical genetics. Part A 38 19353646
2002 Formation of an hER alpha-COUP-TFI complex enhances hER alpha AF-1 through Ser118 phosphorylation by MAPK. The EMBO journal 38 12093745
2019 Long noncoding RNA NR2F1-AS1 enhances the malignant properties of osteosarcoma by increasing forkhead box A1 expression via sponging of microRNA-483-3p. Aging 37 31801112
2003 Repression of the luteinizing hormone receptor gene promoter by cross talk among EAR3/COUP-TFI, Sp1/Sp3, and TFIIB. Molecular and cellular biology 37 12972613
2000 Transcription activation by the orphan nuclear receptor, chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI). Definition of the domain involved in the glucocorticoid response of the phosphoenolpyruvate carboxykinase gene. The Journal of biological chemistry 37 10652338
2022 NR2F1, a Tumor Dormancy Marker, Is Expressed Predominantly in Cancer-Associated Fibroblasts and Is Associated with Suppressed Breast Cancer Cell Proliferation. Cancers 36 35740627
2021 Structural and Functional Aspects of the Neurodevelopmental Gene NR2F1: From Animal Models to Human Pathology. Frontiers in molecular neuroscience 36 34975398
2019 NR2F1-AS1 regulated miR-423-5p/SOX12 to promote proliferation and invasion of papillary thyroid carcinoma. Journal of cellular biochemistry 36 31692033
2010 Genome-wide analysis of binding sites and direct target genes of the orphan nuclear receptor NR2F1/COUP-TFI. PloS one 36 20111703
2004 The pig CYP2E1 promoter is activated by COUP-TF1 and HNF-1 and is inhibited by androstenone. Archives of biochemistry and biophysics 36 15488474
2021 The Parkinson's-disease-associated mutation LRRK2-G2019S alters dopaminergic differentiation dynamics via NR2F1. Cell reports 35 34686322
2019 Sensory cortex wiring requires preselection of short- and long-range projection neurons through an Egr-Foxg1-COUP-TFI network. Nature communications 35 31395862
2017 Gradient COUP-TFI Expression Is Required for Functional Organization of the Hippocampal Septo-Temporal Longitudinal Axis. Cerebral cortex (New York, N.Y. : 1991) 34 26813976
2019 NR2F1-induced NR2F1-AS1 promotes esophageal squamous cell carcinoma progression via activating Hedgehog signaling pathway. Biochemical and biophysical research communications 33 31530388
2016 Upregulation of the Nr2f1-A830082K12Rik gene pair in murine neural crest cells results in a complex phenotype reminiscent of Waardenburg syndrome type 4. Disease models & mechanisms 33 27585883
2000 COUP-TFI (chicken ovalbumin upstream promoter-transcription factor I) regulates cell migration and axogenesis in differentiating P19 embryonal carcinoma cells. Molecular endocrinology (Baltimore, Md.) 33 11117523
2022 NR2F1 Is a Barrier to Dissemination of Early-Stage Breast Cancer Cells. Cancer research 32 35471456
2014 COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration. BMC cancer 32 24906407
2019 Mouse Nr2f1 haploinsufficiency unveils new pathological mechanisms of a human optic atrophy syndrome. EMBO molecular medicine 30 31318166
2007 COUP-TFI modulates estrogen signaling and influences proliferation, survival and migration of breast cancer cells. Breast cancer research and treatment 30 17674191
2022 Hypoxia-induced long noncoding RNA NR2F1-AS1 maintains pancreatic cancer proliferation, migration, and invasion by activating the NR2F1/AKT/mTOR axis. Cell death & disease 29 35283481
2021 NR2F1-AS1/miR-140/HK2 Axis Regulates Hypoxia-Induced Glycolysis and Migration in Hepatocellular Carcinoma. Cancer management and research 28 33488124
2013 COUP-TFI controls activity-dependent tyrosine hydroxylase expression in adult dopaminergic olfactory bulb interneurons. Development (Cambridge, England) 28 24227652
2012 NR2F1 disrupts synergistic activation of the MTTP gene transcription by HNF-4α and HNF-1α. Journal of lipid research 28 22357705
2022 METTL3 inhibits inflammation of retinal pigment epithelium cells by regulating NR2F1 in an m6A-dependent manner. Frontiers in immunology 27 35936005
2020 Long noncoding RNA NR2F1-AS1 promotes the malignancy of non-small cell lung cancer via sponging microRNA-493-5p and thereby increasing ITGB1 expression. Aging 27 32784268
2018 Mitochondrial involvement in a Bosch-Boonstra-Schaaf optic atrophy syndrome patient with a novel de novo NR2F1 gene mutation. Journal of human genetics 27 29410510
2001 EAR2 and EAR3/COUP-TFI regulate transcription of the rat LH receptor. Molecular endocrinology (Baltimore, Md.) 27 11682620
2001 COUP-TFI and COUP-TFII regulate expression of the NHE through a nuclear hormone responsive element with enhancer activity. European journal of biochemistry 26 11168401
2009 ATRA inhibits ceramide kinase transcription in a human neuroblastoma cell line, SH-SY5Y cells: the role of COUP-TFI. Journal of neurochemistry 25 19903244
1995 COUP-TFI is a potential regulator of retinoic acid-modulated development in Xenopus embryos. Mechanisms of development 25 7669692
2015 Transcription factors COUP-TFI and COUP-TFII are required for the production of granule cells in the mouse olfactory bulb. Development (Cambridge, England) 24 25922524
2007 Deoxyribonucleic acid methyl transferases 3a and 3b associate with the nuclear orphan receptor COUP-TFI during gene activation. Molecular endocrinology (Baltimore, Md.) 24 17579209
2020 Long non-coding NR2F1-AS1 is associated with tumor recurrence in estrogen receptor-positive breast cancers. Molecular oncology 23 32392629
1998 COUP-TFI expression in human adrenocortical adenomas: possible role in steroidogenesis. The Journal of clinical endocrinology and metabolism 23 9851803
2023 Tumor cell-intrinsic SETD2 inactivation sensitizes cancer cells to immune checkpoint blockade through the NR2F1-STAT1 pathway. Journal for immunotherapy of cancer 22 38056895
2021 NR2F1-AS1/miR-190a/PHLDB2 Induces the Epithelial-Mesenchymal Transformation Process in Gastric Cancer by Promoting Phosphorylation of AKT3. Frontiers in cell and developmental biology 22 34746118
2017 COUP-TFI mitotically regulates production and migration of dentate granule cells and modulates hippocampal Cxcr4 expression. Development (Cambridge, England) 22 28506990
2021 Pathogenic NR2F1 variants cause a developmental ocular phenotype recapitulated in a mutant mouse model. Brain communications 21 34466801
2020 LncRNA NR2F1-AS1 promotes proliferation and metastasis of ESCC cells via regulating EMT. European review for medical and pharmacological sciences 21 32329844
2020 LncRNA NR2F1-AS1 Regulates miR-371a-3p/TOB1 Axis to Suppress Proliferation of Colorectal Cancer Cells. Cancer biotherapy & radiopharmaceuticals 21 32407174
2012 Genome-wide analysis of androgen receptor targets reveals COUP-TF1 as a novel player in human prostate cancer. PloS one 21 23056316
2005 Dynamic expression of COUP-TFI and COUP-TFII during development and functional maturation of the mouse inner ear. Gene expression patterns : GEP 21 15907456
2018 NR2F1 mediated down-regulation of osteoblast differentiation was rescued by bone morphogenetic protein-2 (BMP-2) in human MSC. Differentiation; research in biological diversity 20 30445268
2022 NR2F1 Regulates TGF-β1-Mediated Epithelial-Mesenchymal Transition Affecting Platinum Sensitivity and Immune Response in Ovarian Cancer. Cancers 18 36230565
2021 Long noncoding RNA NR2F1-AS1 plays a carcinogenic role in gastric cancer by recruiting transcriptional factor SPI1 to upregulate ST8SIA1 expression. Bioengineered 18 34738863
2013 The gene desert mammary carcinoma susceptibility locus Mcs1a regulates Nr2f1 modifying mammary epithelial cell differentiation and proliferation. PLoS genetics 18 23785296
2021 STAT3 activates the transcription of lncRNA NR2F1-AS1 to promote the progression of melanoma via regulating the miR-493-5p/GOLM1 axis. The journal of gene medicine 17 33822440
2020 lncRNA NR2F1-AS1 Regulates miR-17/SIK1 Axis to Suppress the Invasion and Migration of Cervical Squamous Cell Carcinoma Cells. Reproductive sciences (Thousand Oaks, Calif.) 16 31994002
2020 Long non-coding RNA NR2F1-AS1 promoted neuroblastoma progression through miR-493-5p/TRIM2 axis. European review for medical and pharmacological sciences 16 33378023
2009 COUP-TFI and -TFII nuclear receptors are expressed in amacrine cells and play roles in regulating the differentiation of retinal progenitor cells. Experimental eye research 16 19766631
2022 ZEB1-regulated lnc-Nr2f1 promotes the migration and invasion of lung adenocarcinoma cells. Cancer letters 15 35176421
2021 Role of lncRNA NR2F1-AS1 and lncRNA H19 Genes in Hepatocellular Carcinoma and Their Effects on Biological Function of Huh-7. Cancer management and research 15 33568940
2021 The Effect and Mechanism of lncRNA NR2F1-As1/miR-493-5p/MAP3K2 Axis in the Progression of Gastric Cancer. Journal of oncology 15 34335755
2021 NR2F1 database: 112 variants and 84 patients support refining the clinical synopsis of Bosch-Boonstra-Schaaf optic atrophy syndrome. Human mutation 15 34837429
2020 Nr2f1 heterozygous knockout mice recapitulate neurological phenotypes of Bosch-Boonstra-Schaaf optic atrophy syndrome and show impaired hippocampal synaptic plasticity. Human molecular genetics 15 31600777
2020 Long non‑coding RNA NR2F1‑AS1 facilitates the osteosarcoma cell malignant phenotype via the miR‑485‑5p/miR‑218‑5p/BIRC5 axis. Oncology reports 15 32945459
2019 Activation of COUP-TFI by a Novel Diindolylmethane Derivative. Cells 14 30866413

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