Affinage

NOX3

NADPH oxidase 3 · UniProt Q9HBY0

Length
568 aa
Mass
64.9 kDa
Annotated
2026-06-10
31 papers in source corpus 21 papers cited in narrative 21 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NOX3 is a transmembrane NADPH oxidase of the gp91phox/NOX family that generates superoxide and serves as the primary ROS source in the inner ear, where it drives both normal otoconia biogenesis and pathological hearing loss (PMID:15326186, PMID:15014044, PMID:33849947). The enzyme contains predicted transmembrane heme-binding regions and a C-terminal flavoprotein domain with FAD and NADPH binding sites (PMID:11376945), and its catalytic maturation depends critically on p22phox, which it binds and stabilizes and which is required for NOX3 glycosylation, structural maturation, and plasma membrane targeting (PMID:15824103, PMID:17140397). NOX3 produces superoxide constitutively in a p22phox-dependent manner, and its activity is further enhanced by organizer subunits (p47phox or NOXO1) and activator subunits (p67phox or NOXA1) acting through their interaction with p22phox; NOXO1 is uniquely able to activate NOX3 without an activator subunit, and the small GTPase Rac1 modulates activity through the activators rather than being strictly required (PMID:15181005, PMID:15824103, PMID:16507994). In vestibular sensory epithelia NOX3 is indispensable for otoconia morphogenesis, functionally cooperating with the otoconial matrix protein Oc90 to promote calcification (PMID:15014044, PMID:33554930). In the cochlea NOX3 is expressed in supporting cells, hair cells, and spiral ganglion neurons and is induced by cisplatin, aging, and noise, where it acts as the dominant ROS source initiating apoptotic hearing loss through a TRPV1→NOX3→ROS→STAT1 inflammatory pathway (PMID:20214492, PMID:20712533, PMID:33849947). Beyond the ear, NOX3-derived ROS feed into JNK/insulin-resistance, MAPK→Sp1→VEGF, oligodendrocyte differentiation, and retinal signaling contexts (PMID:20102709, PMID:16949073, PMID:27313511, PMID:41339603).

Mechanistic history

Synthesis pass · year-by-year structured walk · 21 steps
  1. 2001 Medium

    Established NOX3 as a candidate superoxide-generating NADPH oxidase by identifying the domain architecture (transmembrane heme regions, FAD/NADPH flavoprotein domain) that defines the gp91phox family.

    Evidence cDNA cloning, sequence/domain analysis, and tissue expression profiling

    PMID:11376945

    Open questions at the time
    • No direct enzymatic activity demonstrated in this study
    • Subcellular localization and regulatory partners unknown
  2. 2004 High

    Localized NOX3 to inner ear sensory epithelia and demonstrated functional superoxide production reconstituted in cells, defining its enzymatic activity and tissue niche.

    Evidence Real-time PCR, in situ hybridization, and HEK-293 reconstitution superoxide assays with subunit co-expression and cisplatin challenge

    PMID:15326186

    Open questions at the time
    • Mechanism of cisplatin enhancement not resolved
    • In vivo relevance not yet tested
  3. 2004 High

    Genetic loss-of-function in mice revealed NOX3 is indispensable for otoconia formation and vestibular function, assigning it a developmental biomineralization role.

    Evidence Allelic series at the head tilt locus with vestibular phenotype characterization

    PMID:15014044

    Open questions at the time
    • Molecular link between NOX3-derived ROS and otoconia mineralization undefined
    • Cell types responsible not pinpointed
  4. 2004 High

    Defined the distinctive subunit regulation of NOX3, showing constitutive activity with p47phox/p67phox and unique activation by NOXO1 alone, distinguishing it from NOX2.

    Evidence Cell-based superoxide assays with subunit combinations and p67phox activation-domain mutagenesis

    PMID:15181005

    Open questions at the time
    • Endogenous physiological activators in the inner ear not identified
    • Structural basis of NOXO1-alone activation unknown
  5. 2005 High

    Identified p22phox as the obligate partner: NOX3 produces superoxide constitutively in a p22phox-dependent manner, binds and stabilizes p22phox, and is Rac-independent.

    Evidence Reciprocal co-immunoprecipitation, p22phox siRNA knockdown, and reconstitution across multiple cell types

    PMID:15824103

    Open questions at the time
    • Stoichiometry of the NOX3–p22phox complex not determined
    • Whether other accessory proteins stabilize the complex unknown
  6. 2005 Medium

    Showed NOXO1 splice variants differentially activate NOX3, indicating regulatory specificity is encoded beyond simple subunit identity.

    Evidence cDNA cloning of NOXO1 splice forms with co-transfection activity and PX-domain lipid-binding assays

    PMID:15949904

    Open questions at the time
    • Molecular determinant of NOXO1gamma's poor NOX3 activation not mapped
    • Single-lab functional assay
  7. 2006 High

    Resolved how Rac1 acts on NOX3, demonstrating it works through the activator subunits (p67phox/NOXA1) rather than NOX3 directly, and that NOX3 promotes p22phox plasma membrane delivery.

    Evidence Dominant-negative Rac1, Rac-binding-deficient NOXA1 mutants, siRNA, and fluorescence microscopy

    PMID:16507994

    Open questions at the time
    • Trafficking machinery routing NOX3/p22phox to the membrane not identified
  8. 2007 High

    Established p22phox as essential for NOX3 maturation, showing it is required for N-linked glycosylation, structural maturation, and membrane targeting, and that membrane NOX3 carries a NOX2-like heme spectrum.

    Evidence In vitro transcription/translation with microsomal glycosylation, p22phox RNAi, Co-IP, and spectrophotometry

    PMID:17140397

    Open questions at the time
    • Order of glycosylation versus heme incorporation during biogenesis unresolved
  9. 2006 Medium

    Extended NOX3 function beyond the ear by placing it in an insulin→ROS→MAPK→Sp1→VEGF signaling cascade in hepatocytes.

    Evidence NOX3 siRNA with H2O2 measurement, MAPK Western blot, Sp1 EMSA, and VEGF-A mRNA quantification in HepG2 cells

    PMID:16949073

    Open questions at the time
    • Single cell line; in vivo relevance untested
    • How insulin activates NOX3 mechanistically unknown
  10. 2010 High

    Demonstrated in vivo that NOX3-derived ROS mediate cisplatin ototoxicity, initiating apoptotic signaling in the cochlea.

    Evidence Transtympanic NOX3 siRNA in rat cochlea with ABR, electron microscopy, TUNEL, and apoptosis marker Western blots

    PMID:20214492

    Open questions at the time
    • Upstream trigger coupling cisplatin to NOX3 induction not defined here
  11. 2010 Medium

    Placed NOX3 within a TRPV1→NOX3→STAT1 inflammatory pathway driving hearing loss, defining its position in the ototoxic signaling hierarchy.

    Evidence UB/OC-1 NOX3 activity and STAT1 reporter assays plus in vivo capsaicin challenge with STAT1 siRNA rescue and ABR

    PMID:20712533

    Open questions at the time
    • Direct biochemical coupling of TRPV1 to NOX3 not shown
    • Single lab
  12. 2010 Medium

    Implicated NOX3 in metabolic signaling via a TNF-α→NOX3→JNK→insulin resistance pathway in hepatocytes.

    Evidence NOX3 siRNA with glycogen assay, ROS measurement, and JNK/IRS1/AKT/GSK phosphorylation Western blots in HepG2

    PMID:20102709

    Open questions at the time
    • Single cell line, no in vivo validation
    • Mechanism of TNF-α-driven NOX3 activation unknown
  13. 2015 Medium

    Linked NOX3 genetically to tonotopic noise vulnerability, showing mutant mice have frequency-specific synaptic ribbon damage.

    Evidence Mouse GWAS (HMDP) with Nox3 mutant validation, DPOAE/ABR, and synaptic ribbon histology

    PMID:25880434

    Open questions at the time
    • Why susceptibility is restricted to 8 kHz unexplained
    • Molecular basis of ribbon synapse damage undefined
  14. 2015 Medium

    Identified a NOX3 role in spermatogonial stem cell self-renewal as a mediator of growth-factor-induced ROS.

    Evidence shRNA Nox3 knockdown with ROS measurement and SSC colony/transplantation assays after FGF2/GDNF stimulation

    PMID:25947060

    Open questions at the time
    • Downstream ROS-responsive effectors in SSCs not identified
    • Single lab
  15. 2016 Medium

    Showed NOX3 expression is held in check by a TLR4-Trif-STAT3/Hsp70 axis in lung endothelium, with derepression driving oxidant injury.

    Evidence Nox3-/-/TLR4-/- double knockouts, endothelial-specific Nox3 silencing, and endothelial Stat3-/- mice with oxidant injury readouts

    PMID:26905942

    Open questions at the time
    • Transcriptional mechanism of STAT3-mediated Nox3 repression not mapped
  16. 2016 Medium

    Implicated NOX3 in oligodendrocyte differentiation within a NOX5→NOX3 sequential ROS-generating network.

    Evidence NOX3/NOX5 siRNA in MO3-13 cells with differentiation marker, ERK/CREB phosphorylation, and PKC inhibition assays

    PMID:27313511

    Open questions at the time
    • Mechanism by which NOX5-derived ROS upregulate NOX3 mRNA unknown
    • Cell line model only
  17. 2016 Medium

    Mapped the cisplatin ototoxicity cascade further upstream, placing adenosine A1 receptor signaling above NOX3 via MAPK-dependent STAT1 Ser727 phosphorylation.

    Evidence In vivo transtympanic A1AR agonist with ABR, STAT1 luciferase reporter, and phospho-STAT1/MAPK Western blots in cochlea and UB/OC-1 cells

    PMID:27053204

    Open questions at the time
    • Whether A1AR regulates NOX3 transcription or activity directly not distinguished
  18. 2019 Low

    Proposed NOX3 as a negative regulator of SHH-driven cerebellar granule precursor proliferation.

    Evidence Genetic mapping of a Nox3 missense mutant with microarray, ROS measurement, and qRT-PCR of SHH targets

    PMID:30853403

    Open questions at the time
    • No functional rescue; pathway directionality not confirmed by orthogonal method
    • Single mutant allele characterization
  19. 2021 High

    Defined the precise cochlear cell types expressing NOX3 and confirmed via knockout that it drives cisplatin-, age-, and noise-induced hearing loss, with the largest contribution to cisplatin ototoxicity.

    Evidence Nox3-Cre;tdTomato reporter and Nox3-KO mice with ABR/DPOAE and immunofluorescence across multiple insult models

    PMID:33849947

    Open questions at the time
    • What induces NOX3 expression upon insult not fully resolved
  20. 2021 Medium

    Demonstrated functional cooperation between NOX3 and the otoconial matrix protein Oc90 in otoconia formation, linking NOX3 ROS output to a defined matrix partner.

    Evidence Double heterozygous/null Oc90;Nox3 mice with vestibular testing plus in vitro calcification in co-transfected cells

    PMID:33554930

    Open questions at the time
    • Biochemical mechanism by which NOX3 and Oc90 enhance calcification unknown
  21. 2025 Medium

    Extended NOX3 function to the retina, showing it is expressed in retinal ganglion and amacrine cells and mediates retinal electrophysiology and cisplatin retinal toxicity.

    Evidence Nox3-Cre;tdTomato reporter and Nox3-KO mice with electroretinography, cell counting, and cisplatin treatment

    PMID:41339603

    Open questions at the time
    • Downstream effectors of NOX3 ROS in retinal cells not identified
    • Single study

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NOX3-derived superoxide is mechanistically transduced into otoconia mineralization versus apoptotic/inflammatory signaling, and what controls its insult-dependent induction, remain unresolved.
  • No structural model of the mature NOX3–p22phox complex
  • Transcriptional regulators driving cisplatin/aging/noise induction undefined
  • Direct ROS targets coupling NOX3 to Oc90-dependent calcification unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016491 oxidoreductase activity 4 GO:0140657 ATP-dependent activity 1
Localization
GO:0005886 plasma membrane 2
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-8953897 Cellular responses to stimuli 3 R-HSA-1266738 Developmental Biology 2
Partners
CYBANCF1NCF2NOXA1NOXO1OC90RAC1
Complex memberships
NOX3–p22phox complex

Evidence

Reading pass · 21 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 NOX3 (GP91-3) encodes a ~65 kDa protein predicted to contain 5–6 transmembrane alpha-helices with heme-binding regions and a flavoprotein homology domain with FAD and NADPH binding sites, classifying it as a superoxide-generating NADPH oxidase homolog of gp91phox. It is expressed primarily in fetal tissues. cDNA cloning, sequence analysis, tissue expression profiling Gene Medium 11376945
2004 NOX3 is highly and selectively expressed in the inner ear (vestibular and cochlear sensory epithelia and spiral ganglions). Transfection of HEK-293 cells with NOX3 demonstrated superoxide production that was enhanced by co-expression with cytoplasmic NOX subunits (p47phox/p67phox or NOXO1/NOXA1). NOX3-dependent superoxide production required a stimulus without subunits or with phagocyte subunits, but was stimulus-independent with NOXO1/NOXA1. Cisplatin pre-incubation markedly enhanced NOX3-dependent superoxide production. Real-time PCR, microdissection, in situ hybridization, HEK-293 cell transfection, superoxide assay The Journal of biological chemistry High 15326186
2004 Loss-of-function mutations in Nox3 in mice result in absence of otoconia (biomineral particles in the utricle and saccule) and profound vestibular dysfunction, establishing Nox3 as indispensable for otoconia morphogenesis in the inner ear. Genetic allelic series in mice (head tilt locus), vestibular phenotype characterization Genes & development High 15014044
2004 Nox3 is activated by regulatory subunits p47phox/p67phox (like gp91phox) and by NOXO1 alone (unlike gp91phox). Nox3 activity with p47phox/p67phox is constitutively high (not requiring PMA stimulation). NOXO1 strongly activates Nox3 without requiring NOXA1 or p67phox, and the p67phox activation domain mutation (V204A) that abolishes gp91phox activation still supports Nox3 activation. Cell-based superoxide production assays, transient transfection of HEK-293 cells with Nox subunit combinations, PMA stimulation assays The Journal of biological chemistry High 15181005
2005 Nox3 constitutively produces superoxide in a p22phox-dependent manner without requiring organizer or activator subunits. Nox3 physically interacts with and stabilizes p22phox. Organizers p47phox and Noxo1 enhance Nox3 activity via their interaction with p22phox. The small GTPase Rac, essential for gp91phox/Nox2 activity, is dispensable for Nox3 activity. Cell-based superoxide assays, co-immunoprecipitation, siRNA knockdown of p22phox, transfection of multiple cell types The Journal of biological chemistry High 15824103
2006 Rac1 regulates Nox3 activity through the Nox activators (p67phox or Noxa1); Rac1 dominant-negative mutants, Noxa1 mutants defective in Rac binding, and siRNA-mediated Rac1 silencing all inhibit Nox3. Nox3 expression promotes p22phox transport to the plasma membrane. Nox3 activity is inhibited by mutations in the p22phox-binding SH3 domains of organizers (p47phox or Noxo1). Plasma membrane targeting of Noxa1 depends on Noxo1 via tail-to-tail interactions. Cell transfection, dominant-negative Rac1 mutants, siRNA, Noxa1 Rac-binding mutants, fluorescence microscopy Molecular and cellular biology High 16507994
2007 p22phox is required for glycosylation, structural maturation, and plasma membrane targeting of Nox3. p22phox co-precipitates with both Nox3 and NoxO1. RNAi-mediated reduction of p22phox decreased Nox3 activity. In vitro translation of Nox3 cDNA produced an ~50 kDa primary product that underwent N-linked glycosylation. The heme spectrum of Nox3 in plasma membrane is identical to that of Nox2. RNAi knockdown of p22phox, co-immunoprecipitation, in vitro transcription/translation with microsomal glycosylation assay, spectrophotometry, HEK-293 transfection The Biochemical journal High 17140397
2005 NOXO1 splice forms differ in regulation of Nox3: NOXO1beta activates Nox3 effectively, whereas NOXO1gamma shows a significantly poorer ability to activate Nox3, despite both activating Nox1 and having similar lipid-binding properties of their PX domains. cDNA cloning of splice variants, Nox activity assay with co-transfection, PX domain lipid-binding assay Gene Medium 15949904
2010 NOX3 siRNA knockdown in the rat cochlea (transtympanic delivery) prevented cisplatin-induced outer hair cell damage, reduced threshold shifts, reduced apoptosis (decreased Bax, restored Bcl2), and reduced expression of cochlear damage biomarkers TRPV1 and KIM-1, demonstrating that NOX3-derived ROS mediate cisplatin ototoxicity and initiate apoptotic signaling in the cochlea. In vivo siRNA knockdown, auditory brainstem response, scanning electron microscopy, immunohistochemistry, TUNEL assay, Western blot Antioxidants & redox signaling High 20214492
2010 TRPV1 activation by capsaicin increases NOX3 NADPH oxidase activity and STAT1 activation in cochlear cells (UB/OC-1). In vivo, capsaicin-induced hearing loss was associated with STAT1 activation and inflammatory cell infiltration. STAT1 siRNA protected against capsaicin-induced hearing loss, placing NOX3 upstream of STAT1 in a TRPV1→NOX3→STAT1 inflammatory pathway. UB/OC-1 cell NOX3 activity assays, STAT1 reporter assay, in vivo capsaicin intratympanic injection, STAT1 siRNA knockdown, auditory brainstem response Antioxidants & redox signaling Medium 20712533
2010 TNF-α activates NOX3 in HepG2 hepatocytes to generate ROS; NOX3 siRNA prevents TNF-α-induced decrease of cellular glycogen by blocking NOX3-dependent JNK activation, IRS1 inhibition, and reduction of AKT/GSK phosphorylation, placing NOX3 in a TNF-α→NOX3→ROS→JNK→insulin resistance signaling pathway in hepatocytes. siRNA knockdown of NOX3, glycogen assay, Western blot for JNK/IRS1/AKT/GSK phosphorylation, ROS measurement FEBS letters Medium 20102709
2016 Adenosine A1 receptor (A1AR) agonist R-PIA suppresses cisplatin-induced NOX3 expression and ROS generation in the cochlea, reduces STAT1 phosphorylation at Ser727 (but not Tyr701) via inhibition of ERK1/2, p38, and JNK MAPK pathways, and decreases TNF-α, iNOS, and COX-2 expression, placing A1AR upstream of NOX3 in the cisplatin ototoxicity signaling cascade. In vivo transtympanic A1AR agonist administration, auditory brainstem response, scanning electron microscopy, STAT1 luciferase reporter assay, Western blot for phospho-STAT1/MAPK, UB/OC-1 cell culture The Journal of neuroscience Medium 27053204
2016 NOX3 and NOX5 are required for ROS-mediated oligodendrocyte differentiation; selective depletion of NOX3 or NOX5 inhibits PMA-induced differentiation markers (Olig-2, MBP). NOX5 silencing downregulates NOX3 mRNA levels, suggesting NOX5-derived ROS upregulate NOX3 expression as part of a sequential ROS-generating network driving oligodendrocyte differentiation. siRNA knockdown of NOX3 and NOX5 in MO3-13 cells, Western blot for differentiation markers, ERK/CREB phosphorylation assays, PKC inhibition Frontiers in cellular neuroscience Medium 27313511
2016 In lung endothelial cells, NOX3 expression is suppressed by a TLR4-Trif-STAT3 signaling axis, and Hsp70 acting via TLR4 suppresses Nox3. When this pathway is disrupted (TLR4 knockout), Nox3 is induced and drives increased oxidant injury and apoptosis. Endothelial-specific Nox3 silencing rescued the pro-oxidant phenotype of TLR4 knockout mice. Nox3-/-/TLR4-/- double knockout mice, endothelial-targeted lentiviral Nox3 silencing constructs, endothelial-targeted Stat3-/- mice, oxidant injury measurements Antioxidants & redox signaling Medium 26905942
2006 NOX3 mediates insulin-induced VEGF-A expression in HepG2 cells. NOX3 siRNA knockdown abrogates insulin-stimulated H2O2 production, inhibits the second phase of p42/44 MAPK phosphorylation, reduces Sp1 DNA binding, and prevents VEGF-A mRNA upregulation, placing NOX3 in an insulin→NOX3→ROS→MAPK→Sp1→VEGF pathway. siRNA knockdown of NOX3, H2O2 measurement, MAPK phosphorylation Western blot, Sp1 EMSA, VEGF-A mRNA quantification Experimental cell research Medium 16949073
2015 Nox3 mutant and heterozygous mice show greater susceptibility to noise-induced hearing loss specifically at 8 kHz, with damage localized to synaptic ribbons of the cochlea at that frequency, genetically linking Nox3 to tonotopic susceptibility to noise-induced cochlear damage. Mouse GWAS (HMDP), Nox3 mutant validation, DPOAE and ABR threshold measurements, histological analysis of synaptic ribbons PLoS genetics Medium 25880434
2015 Nox3 is transiently induced by FGF2 and GDNF stimulation in spermatogonial stem cells (SSCs). ShRNA-mediated Nox3 inhibition reduced cytokine-induced ROS generation and decreased SSC numbers in culture and in freshly isolated testis cells, establishing Nox3 as a mediator of self-renewal factor-induced ROS generation required for SSC self-renewal. shRNA knockdown of Nox3, ROS measurement, SSC colony/transplantation assays, qRT-PCR Biology of reproduction Medium 25947060
2019 A missense mutation in Nox3 (p.Asn64Tyr) in mice causes increased proliferation of cerebellar granule cell precursors associated with upregulation of SHH target genes Gli1-3 and CyclinD1, and elevated ROS production, placing Nox3 as a negative regulator of SHH pathway-driven neural progenitor proliferation in the cerebellum. Genetic mapping, cDNA microarray, ROS measurement, qRT-PCR of SHH targets in Nox3 mutant vs. wild-type cerebella Biochimica et biophysica acta. Molecular basis of disease Low 30853403
2021 Using Nox3-Cre knock-in reporter mice, Nox3-expressing cells in the cochlea were identified as supporting cells, outer hair cells, inner hair cells, and spiral ganglion neurons. Nox3 expression increased with cisplatin, aging, and noise insults. Nox3 knockout mice showed reduced cisplatin-induced, age-related, and noise-induced hearing loss, with the greatest Nox3 contribution to cisplatin-induced hearing loss. Nox3-Cre;tdTomato knock-in reporter mice, Nox3-KO mice, ABR/DPOAE hearing assessment, immunofluorescence, cisplatin/noise/aging models The Journal of neuroscience High 33849947
2021 Oc90 (otoconin-90) and Nox3 functionally cooperate in otoconia formation; double heterozygous Oc90/Nox3 mice show severe imbalance and otoconia defects, while single heterozygotes are normal. Co-expression of Oc90 and Nox3 in vitro produces markedly enhanced calcification compared to either protein alone. Double heterozygous and double null mutant mouse generation, vestibular behavioral and electrophysiological testing, morphological analysis, in vitro calcification assay in co-transfected cells Journal of vestibular research Medium 33554930
2025 Nox3 is expressed in retinal ganglion cells (RGCs) and GABAergic amacrine cells (ACs) as determined by Nox3-Cre;tdTomato reporter mice. Nox3-KO mice show reduced ERG a-, b-, and STR-waves, indicating a functional role in retinal electrophysiology. Cisplatin reduced Nox3-expressing RGC/AC numbers in heterozygous but not full Nox3-KO mice, indicating Nox3-derived ROS mediate cisplatin retinal toxicity. Nox3-Cre;tdTomato knock-in reporter mice, Nox3-KO mice, electroretinography, cell counting, cisplatin treatment Cellular and molecular life sciences Medium 41339603

Source papers

Stage 0 corpus · 31 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4, and Nox5. Gene 681 11376945
2004 NOX3, a superoxide-generating NADPH oxidase of the inner ear. The Journal of biological chemistry 349 15326186
2004 Vestibular defects in head-tilt mice result from mutations in Nox3, encoding an NADPH oxidase. Genes & development 222 15014044
2006 Involvement of Rac1 in activation of multicomponent Nox1- and Nox3-based NADPH oxidases. Molecular and cellular biology 195 16507994
2005 The NADPH oxidase Nox3 constitutively produces superoxide in a p22phox-dependent manner: its regulation by oxidase organizers and activators. The Journal of biological chemistry 153 15824103
2004 Nox3 regulation by NOXO1, p47phox, and p67phox. The Journal of biological chemistry 129 15181005
2010 Transtympanic administration of short interfering (si)RNA for the NOX3 isoform of NADPH oxidase protects against cisplatin-induced hearing loss in the rat. Antioxidants & redox signaling 109 20214492
2016 Adenosine A1 Receptor Protects Against Cisplatin Ototoxicity by Suppressing the NOX3/STAT1 Inflammatory Pathway in the Cochlea. The Journal of neuroscience : the official journal of the Society for Neuroscience 97 27053204
2010 NOX3 NADPH oxidase couples transient receptor potential vanilloid 1 to signal transducer and activator of transcription 1-mediated inflammation and hearing loss. Antioxidants & redox signaling 81 20712533
2007 Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3. The Biochemical journal 70 17140397
2015 Genome-wide association study identifies nox3 as a critical gene for susceptibility to noise-induced hearing loss. PLoS genetics 63 25880434
2016 Reactive Oxygen Species Derived from NOX3 and NOX5 Drive Differentiation of Human Oligodendrocytes. Frontiers in cellular neuroscience 58 27313511
2005 Alternative mRNA splice forms of NOXO1: differential tissue expression and regulation of Nox1 and Nox3. Gene 46 15949904
2006 Insulin-induced vascular endothelial growth factor expression is mediated by the NADPH oxidase NOX3. Experimental cell research 45 16949073
2015 ROS-Generating Oxidase Nox3 Regulates the Self-Renewal of Mouse Spermatogonial Stem Cells. Biology of reproduction 42 25947060
2021 Nox3-Derived Superoxide in Cochleae Induces Sensorineural Hearing Loss. The Journal of neuroscience : the official journal of the Society for Neuroscience 39 33849947
2012 siRNA-mediated knock-down of NOX3: therapy for hearing loss? Cellular and molecular life sciences : CMLS 39 22562580
2010 NOX3-derived reactive oxygen species promote TNF-alpha-induced reductions in hepatocyte glycogen levels via a JNK pathway. FEBS letters 36 20102709
2019 Pancreastatin inhibitor PSTi8 attenuates hyperinsulinemia induced obesity and inflammation mediated insulin resistance via MAPK/NOX3-JNK pathway. European journal of pharmacology 21 31586632
2016 An Endothelial Hsp70-TLR4 Axis Limits Nox3 Expression and Protects Against Oxidant Injury in Lungs. Antioxidants & redox signaling 20 26905942
2023 Diallyl trisulfide inhibits monosodium urate-induced NLRP3 inflammasome activation via NOX3/4-dependent mitochondrial oxidative stress in RAW 264.7 and bone marrow-derived macrophages. Phytomedicine : international journal of phytotherapy and phytopharmacology 18 36796188
2005 Evaluation of two anti-gp91phox antibodies as immunoprobes for Nox family proteins: mAb 54.1 recognizes recombinant full-length Nox2, Nox3 and the C-terminal domains of Nox1-4 and cross-reacts with GRP 58. Biochimica et biophysica acta 15 16140048
2010 Molecular characterization of an allelic series of mutations in the mouse Nox3 gene. Mammalian genome : official journal of the International Mammalian Genome Society 13 21161235
2019 Mutation in NADPH oxidase 3 (NOX3) impairs SHH signaling and increases cerebellar neural stem/progenitor cell proliferation. Biochimica et biophysica acta. Molecular basis of disease 10 30853403
2017 Rare NOX3 Variants Confer Susceptibility to Agranulocytosis During Thyrostatic Treatment of Graves' Disease. Clinical pharmacology and therapeutics 9 28486791
2018 Mouse Magnetic-field Nystagmus in Strong Static Magnetic Fields Is Dependent on the Presence of Nox3. Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology 8 30444848
2022 Development and in vivo validation of small interfering RNAs targeting NOX3 to prevent sensorineural hearing loss. Frontiers in neurology 6 36188374
2021 Functional cooperation between two otoconial proteins Oc90 and Nox3. Journal of vestibular research : equilibrium & orientation 4 33554930
2021 [Association between polymorphism of CASP and NOX3 with risk of noise-induced hearing loss]. Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 3 34886640
2025 Nox3 expression and function in retinal ganglion cells and Amacrine cells. Cellular and molecular life sciences : CMLS 1 41339603
2025 Epigallocatechin-3-gallate Restores X-irradiation-Induced Impairments in Cognitive Function and Hippocampal Neurogenesis by Suppressing the TLR4-NOX3/4 and ROS-NF-κB Pathways in Microglia. Molecular neurobiology 0 41317232

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