Affinage

NEIL1

Endonuclease 8-like 1 · UniProt Q96FI4

Length
390 aa
Mass
43.7 kDa
Annotated
2026-06-10
100 papers in source corpus 40 papers cited in narrative 40 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NEIL1 is a bifunctional DNA glycosylase/AP lyase that initiates base excision repair by excising a broad spectrum of oxidatively damaged bases — including thymine glycol, FapyA/FapyG formamidopyrimidines, 5-hydroxyuracil, dihydrouracil, and the further-oxidized hydantoin lesions guanidinohydantoin and spiroiminodihydantoin — via beta,delta-elimination, with cellular loss conferring sensitivity to oxidative and ionizing-radiation damage (PMID:12713815, PMID:15595846, PMID:15533836). Unlike NTH1 and OGG1, it efficiently processes lesions in single-stranded, bubble, and fork-structured DNA as well as duplex, quadruplex, and three-/four-stranded crosslinked substrates, extending its repertoire to psoralen interstrand crosslink unhooking and to 8,5'-cyclopurine and AFB1-Fapy-dG adducts (PMID:14522990, PMID:19258314, PMID:20067321, PMID:28373545, PMID:29234069). Its catalytic mechanism depends on an N-terminal Pro2/Lys54 nucleophile and a 'zincless finger' motif in which a conserved arginine, rather than coordinated zinc, is required for activity (PMID:15232006, PMID:22858590). NEIL1 functions as a replication-coupled repair factor: it is recruited to S-phase replication foci and assembles a 'BERosome' through its intrinsically disordered C-terminal domain, which mediates interactions with replication and repair proteins including PCNA, RFC, pol delta, FEN-1, RPA, DNA ligase I, WRN, CSB, the 9-1-1 clamp, hnRNP-U, and PARP-1, most of which reciprocally stimulate its glycosylase activity to enable pre-replicative repair of oxidized bases ahead of the fork (PMID:18032376, PMID:17611195, PMID:17395641, PMID:18662981, PMID:20338831, PMID:22902625, PMID:23898192, PMID:26134572). NEIL1 activity, abundance, and localization are tuned by post-translational and RNA-level controls: p300 acetylation of C-terminal lysines drives chromatin association and enhances activity (PMID:29698889), Mule- and TRIM26-mediated polyubiquitylation governs steady-state protein levels and radiation resistance (PMID:27924031), and ADAR1 RNA editing at codon 242 (Lys→Arg) reshapes substrate specificity (PMID:21068368). NEIL1 additionally localizes to mitochondria, where it interacts with mtSSB and TFAM to support mitochondrial genome integrity and gene expression (PMID:29522991, PMID:35938152). In vivo, Neil1 deficiency increases endogenous lesion burden, raises mutation frequency, modifies trinucleotide repeat instability, and confers strong susceptibility to AFB1-induced hepatocellular carcinoma with a characteristic mutational signature (PMID:18495559, PMID:22914735, PMID:28373545, PMID:38779538).

Mechanistic history

Synthesis pass · year-by-year structured walk · 22 steps
  1. 2003 High

    Established NEIL1 as a functional DNA repair enzyme by defining it as a bifunctional glycosylase/AP lyase that excises oxidized bases and protects cells from oxidative DNA damage.

    Evidence In vitro glycosylase assays on a substrate panel plus RNAi knockdown with gamma-irradiation survival in embryonic stem cells

    PMID:12713815

    Open questions at the time
    • Did not resolve preference among substrate classes
    • Cellular pathway context and partners unknown
  2. 2003 High

    Distinguished NEIL1 from other glycosylases by showing it preferentially acts on non-duplex DNA (bubble and ssDNA), hinting at a replication/transcription-coupled role.

    Evidence Comparative in vitro glycosylase and binding assays across duplex, bubble, and ssDNA substrates

    PMID:14522990

    Open questions at the time
    • Did not identify the in vivo source of such structures
    • No partner proteins linking NEIL1 to replication forks yet
  3. 2004 High

    Defined the structural basis of catalysis by identifying a 'zincless finger' motif required for activity, explaining how NEIL1 engages lesions without a canonical zinc finger.

    Evidence X-ray crystallography of human NEIL1 with active-site mutagenesis and activity assays

    PMID:15232006

    Open questions at the time
    • No lesion-bound or full-length structure including the disordered C-terminus
    • Mechanism of broad substrate recognition not fully resolved
  4. 2004 High

    Extended the catalogue of physiological substrates and showed NEIL1 can functionally relieve OGG1 product inhibition, positioning it within the broader BER network.

    Evidence In vitro assays on gamma-irradiated DNA with GC-MS quantification and OGG1 turnover/displacement assays

    PMID:15350146 PMID:15595846

    Open questions at the time
    • OGG1 cooperation shown without stable physical interaction (Medium)
    • Physiological significance of OGG1 displacement untested in cells
  5. 2005 High

    Broadened NEIL1's lesion spectrum to hyperoxidized guanine products and lesions near strand breaks, defining substrates other glycosylases cannot process.

    Evidence In vitro glycosylase and trapping assays on Gh/Sp and 3'-proximal-lesion oligonucleotides

    PMID:15533836 PMID:16129732

    Open questions at the time
    • 3'-proximal-lesion processing replicated in only one lab (Medium)
    • In vivo relevance of hydantoin repair not established here
  6. 2005 Medium

    Identified the transcriptional control of NEIL1, showing oxidative stress induces it via CRE/AP-1 promoter elements binding c-Jun and CREB/ATF2.

    Evidence Luciferase reporters, transcription start mapping, EMSA, and phospho-c-Jun immunoblot in human cells

    PMID:16118226

    Open questions at the time
    • Single-lab promoter analysis
    • Whether induction measurably alters repair capacity not shown
  7. 2007 High

    Connected NEIL1 mechanistically to DNA replication by demonstrating direct, activity-stimulating interactions with PCNA, WRN, and the 9-1-1 clamp through its C-terminal domain.

    Evidence Reciprocal Co-IP, quantitative binding, in vitro stimulation, co-localization, domain mapping, and siRNA epistasis

    PMID:17395641 PMID:17611195 PMID:18032376

    Open questions at the time
    • C-terminal interaction interface lacks a canonical PIP box, recruitment mechanism unresolved
    • Spatiotemporal ordering of these interactions at the fork unknown
  8. 2008 High

    Linked NEIL1 to long-patch BER via FEN-1 and demonstrated its endogenous anti-mutator role at oxidized A:T sites.

    Evidence Co-IP, quantitative binding, LP-BER reconstitution, and Hprt mutation-frequency assay under oxidative stress

    PMID:18495559 PMID:18662981

    Open questions at the time
    • Hprt mutation study used antisense knockdown in a single lab (Medium)
    • Relative contribution of short- vs long-patch BER in cells unquantified
  9. 2009 High

    Expanded NEIL1's pathway roles into interstrand crosslink repair and showed CSB-stimulated FapyGua repair, embedding NEIL1 in transcription-coupled and crosslink repair.

    Evidence In vitro BER reconstitution on crosslinked substrates; Co-IP, stimulation, double-knockdown epistasis, and knockout-mouse lesion quantification for CSB

    PMID:19179336 PMID:19258314

    Open questions at the time
    • In vivo coupling of NEIL1 to XPF/ERCC1 ICL repair not directly tested
    • How CSB recruits NEIL1 to chromatin unknown
  10. 2010 High

    Revealed multilayered regulation of NEIL1 at the fork and at the RNA level — RPA inhibition relieved by PCNA, and ADAR1 RNA editing reprogramming substrate specificity.

    Evidence Co-IP, quantitative binding, modulation assays for RPA; in vitro kinetics of edited vs unedited NEIL1 with ADAR1 identification by siRNA

    PMID:20214901 PMID:20338831 PMID:21068368

    Open questions at the time
    • 8-oxoAde abortive-repair finding is Medium-confidence single-lab
    • Physiological fraction of edited NEIL1 in tissues not established
  11. 2010 Medium

    Implicated NEIL1 in a BER-independent NER-like pathway for cyclopurine lesions, broadening its repair repertoire.

    Evidence Lesion accumulation (R/S-cdA) in Neil1-/- but not Ogg1-/- mouse liver by GC-MS/MS

    PMID:20067321

    Open questions at the time
    • Mechanism inferred from accumulation, not enzymatic reconstitution
    • Direct NEIL1 role in NER not demonstrated
  12. 2012 High

    Defined further partner interactions (PARP-1, hnRNP-U) and a disease-modifier role, with systematic mapping of catalytic residues.

    Evidence Co-IP, binding, stimulation, epistasis for PARP-1/hnRNP-U; Neil1-/- HD-model repeat-expansion genetics; site-directed mutagenesis with single-turnover kinetics

    PMID:22858590 PMID:22902625 PMID:22914735 PMID:23104860 PMID:23926102

    Open questions at the time
    • PARP-1 interaction is Medium-confidence single-lab
    • How NEIL1-initiated BER mechanistically drives repeat expansion in vivo not fully resolved
  13. 2013 High

    Established NEIL1 as a replication-fork-resident repair factor that performs pre-replicative repair, interacting with the replication machinery via its C-terminus.

    Evidence Gel filtration, Co-IP with RFC/pol delta/ligase I, co-localization with RPA/BrdU foci, in vitro primer-extension inhibition, and nascent-chain analysis

    PMID:23898192

    Open questions at the time
    • Whether fork-coupled repair is the dominant cellular pathway not quantified
    • Trigger for NEIL1 fork recruitment unresolved
  14. 2013 Medium

    Characterized the disordered C-terminal domain as an intramolecular stabilizer, providing biophysical basis for its dual structural and interaction-hub roles.

    Evidence Intrinsic fluorescence, SAXS, CD, osmolyte stability assays, and Lys-cluster mutagenesis

    PMID:23542007

    Open questions at the time
    • Single-lab biophysical study
    • Link between CTD-mediated stability and in-cell function not directly shown
  15. 2015 High

    Demonstrated the C-terminal domain is essential for assembling the replication-coupled 'BERosome' and chromatin/foci association, with a CTD fragment acting as dominant-negative.

    Evidence Co-IP, chromatin fractionation, foci co-localization, in vitro BER reconstitution, and dominant-negative ROS-sensitivity in cells

    PMID:26134572

    Open questions at the time
    • Stoichiometry and architecture of the assembled BERosome unresolved
    • Order of partner assembly during S phase unknown
  16. 2016 Medium

    Identified JNK1 phosphorylation of NEIL1 and a phosphomimetic Y263 inactivating site, adding a kinase-signaling layer to NEIL1 regulation.

    Evidence MS phosphosite mapping, Co-IP, in vitro kinase assay, and mutagenesis activity assays

    PMID:27518429

    Open questions at the time
    • Functional role of S61/S207/S306 phosphorylation unclear (no effect on activity)
    • Y263 modification status in cells not demonstrated
  17. 2016 High

    Defined ubiquitin-dependent control of NEIL1 abundance, linking its turnover to radiation resistance.

    Evidence In vitro ubiquitylation, siRNA stability assays for Mule and TRIM26, and clonogenic survival after IR

    PMID:27924031

    Open questions at the time
    • Signals triggering ligase selection between Mule and TRIM26 unknown
    • Deubiquitylase counterpart not identified
  18. 2017 High

    Mechanistically tied NEIL1 to the replication-to-repair switch and to epigenetic demethylation, and expanded crosslink and adduct substrates.

    Evidence Biophysical demonstration of NEIL1-driven PCNA trimer destabilization (SEC/MS/AFM/SAXS); in vitro assays on 5caC/TDG, AFB1-Fapy-dG, and four-stranded ICLs; Neil1-/- mouse carcinogenesis bioassay

    PMID:27994037 PMID:28373545 PMID:28827588 PMID:29234069

    Open questions at the time
    • 5caC/TDG cooperation and four-stranded ICL processing are Medium-confidence single-lab
    • In vivo significance of PCNA monomerization not directly tested
  19. 2018 High

    Established acetylation as a master regulator coupling NEIL1 localization to function and demonstrated mitochondrial localization with mtSSB interaction.

    Evidence In vitro p300 acetylation, site mutagenesis, fractionation/IF, and activity/survival assays; mitochondrial fractionation with protein-painting, far-western, MALS, and SAXS for mtSSB

    PMID:29522991 PMID:29698889

    Open questions at the time
    • mtSSB interaction is Medium-confidence single-lab
    • Deacetylase and dynamic regulation of acetylation in vivo not defined
  20. 2019 High

    Defined a developmental and mitochondrial-protective role and refined the division of labor between NEIL1 and NEIL3 at replication forks.

    Evidence Xenopus/mouse ESC Neil knockout with TP53 epistasis and mtDNA damage assays; comparative in vitro fork-substrate glycosylase assays and AFB1/NM-Fapy-dG kinetics

    PMID:31018584 PMID:31566562 PMID:31733589

    Open questions at the time
    • Fork division-of-labor and edited-NEIL1 kinetics are Medium-confidence in vitro models
    • How mtDNA repair couples to neural crest survival mechanistically not fully resolved
  21. 2022 Medium

    Linked NEIL1 to mitochondrial transcription by showing it interacts with TFAM and modulates TFAM-DNA complexes and mitochondrial gene expression under damage.

    Evidence Co-precipitation, HDX-MS, MALS, gel filtration, and mRNA expression in NEIL1-deficient cells

    PMID:35938152

    Open questions at the time
    • Single-lab, transient interaction
    • Whether the effect reflects repair or a direct transcriptional role is unresolved
  22. 2024 High

    Provided high-resolution in vivo confirmation that NEIL1 suppresses AFB1-induced nuclear mutagenesis matching a defined COSMIC signature.

    Evidence Duplex sequencing of Neil1-/- mouse liver after AFB1 with tumor bioassay

    PMID:38779538

    Open questions at the time
    • Why mitochondrial genome was not similarly affected unexplained
    • Human relevance of the mouse mutational signature not directly tested

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the BERosome is spatiotemporally assembled and how the multiple regulatory layers (acetylation, ubiquitylation, phosphorylation, RNA editing) are integrated to direct NEIL1 between nuclear replication-coupled repair and mitochondrial maintenance remains unresolved.
  • No integrated structure of the assembled NEIL1 BERosome
  • Hierarchy and crosstalk among PTMs not established
  • Determinants of nuclear vs mitochondrial partitioning unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140097 catalytic activity, acting on DNA 8 GO:0003677 DNA binding 4 GO:0098772 molecular function regulator activity 4 GO:0016787 hydrolase activity 3
Localization
GO:0005634 nucleus 3 GO:0005739 mitochondrion 3 GO:0000228 nuclear chromosome 2
Pathway
R-HSA-69306 DNA Replication 4 R-HSA-73894 DNA Repair 4 R-HSA-1643685 Disease 2
Partners
CSBFEN1HNRNPUPARP1PCNARPATFAMWRN
Complex memberships
9-1-1 (Rad9-Rad1-Hus1) clamp-associatedBERosome (NEIL1-replication/repair protein complex)

Evidence

Reading pass · 40 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 NEIL1 is a bifunctional DNA glycosylase/AP lyase that excises oxidatively damaged bases (including thymine glycol stereoisomers, FapyA, FapyG, 5,6-dihydrouracil) from DNA and initiates base excision repair; RNA interference knockdown of Neil1 in embryonic stem cells conferred sensitivity to gamma-irradiation. In vitro glycosylase assay with purified recombinant mNeil1, substrate specificity panel, RNAi knockdown + gamma-irradiation cell survival assay DNA repair High 12713815
2003 NEIL1 (and NEIL2) preferentially excise oxidized base lesions from DNA bubble and single-stranded DNA substrates, in contrast to NTH1 and OGG1 which require duplex DNA; NEIL1 efficiently excises 5-hydroxyuracil from bubble and ssDNA but not 8-oxoguanine from bubble structures. In vitro DNA glycosylase assays comparing activity on duplex, bubble, and single-stranded DNA substrates; affinity binding assays The Journal of biological chemistry High 14522990
2004 Crystal structure of human NEIL1 revealed a 'zincless finger' motif — two antiparallel beta-strands mimicking the zinc-finger hairpin of Fpg/Nei family members but lacking zinc-coordinating residues; mutagenesis of a conserved arginine in this motif greatly reduces glycosylase activity, establishing the zincless finger as required for NEIL1 activity. X-ray crystallography; active-site mutagenesis with glycosylase activity assay Proceedings of the National Academy of Sciences of the United States of America High 15232006
2004 Mouse NEIL1 specifically excises FapyGua and FapyAde from high-molecular-weight DNA containing multiple lesions generated by ionizing radiation, with equal specificity for both formamidopyrimidines; also excises thymine glycol and 5-hydroxy-5-methylhydantoin at lower rates. In vitro enzymatic assay on gamma-irradiated high-MW DNA; GC-MS quantification of released lesions Biochemistry High 15595846
2004 NEIL1 stimulates OGG1 turnover at abasic (AP) sites by displacing bound OGG1 and performing beta-delta elimination at the AP site, analogous to APE1; this functional collaboration does not require stable protein–protein interaction between NEIL1 and OGG1. In vitro turnover/displacement assay; AP lyase activity assay; protein interaction assessment Biochemistry Medium 15350146
2005 NEIL1 and NEIL2 recognize and excise the further-oxidized 8-oxoG lesions guanidinohydantoin (Gh/Ia) and spiroiminodihydantoin (Sp) from both single-stranded and duplex DNA via beta- and delta-elimination mechanism; NEIL1 excises Sp from all four base contexts in duplex DNA. In vitro DNA glycosylase assays; DNA trapping studies; lesion-containing oligonucleotide substrates DNA repair High 15533836
2005 NEIL1 excises oxidized base lesions located in close proximity (3' end) to a DNA single-strand break — a substrate that NTH1 and OGG1 cannot efficiently process. In vitro DNA glycosylase assay on oligonucleotide substrates with 3'-proximal lesions Nucleic acids research Medium 16129732
2005 Reactive oxygen species transcriptionally induce NEIL1 expression in human cells via CRE/AP-1 sites in the promoter that bind c-Jun and CREB/ATF2; oxidative stress increases binding of phospho-c-Jun to the NEIL1 promoter. Luciferase reporter assay; transcription start site mapping; EMSA with cell extracts; phospho-c-Jun immunoblot The Journal of biological chemistry Medium 16118226
2007 Human NEIL1 physically interacts with PCNA both in vivo and in vitro; PCNA stimulates NEIL1 glycosylase activity on 5-hydroxyuracil in ssDNA and fork-structured substrates by enhancing NEIL1 loading on substrate; the interaction domain maps to a C-terminal region (lacking canonical PIP box) conserved with DNA polymerase delta. Co-immunoprecipitation (in vivo and in vitro), mammalian two-hybrid, in vitro activity stimulation assay; domain-mapping by deletion mutants The Journal of biological chemistry High 18032376
2007 Werner syndrome protein (WRN) physically associates with NEIL1 (KD ~60 nM) via C-terminal residues 288–349 of NEIL1 and the RQC domain of WRN, independent of WRN helicase activity; WRN stimulates NEIL1 excision of oxidative lesions from bubble DNA; WRN-depleted cells accumulate 8-oxoG, FapyG, and FapyA, and combined WRN/NEIL1 knockdown does not show additive damage accumulation, placing WRN and NEIL1 in the same repair pathway. Co-immunoprecipitation; in vitro binding (Kd measurement); in vitro stimulation assay; nuclear co-localization; genetic epistasis via siRNA double-knockdown; lesion quantification by mass spectrometry The Journal of biological chemistry High 17611195
2007 Human NEIL1 interacts with the 9-1-1 checkpoint clamp (Rad9-Rad1-Hus1) via residues 290–350; individual 9-1-1 subunits and the intact complex stimulate NEIL1 DNA glycosylase activity; NEIL1 nuclear foci co-localize with Rad9 foci after H2O2 treatment. Co-immunoprecipitation; in vitro binding and stimulation assay; nuclear co-localization by immunofluorescence; domain mapping Nucleic acids research High 17395641
2008 NEIL1 physically interacts with flap endonuclease 1 (FEN-1) via the disordered C-terminal region of NEIL1 (KD ~0.2 µM) and the disordered C-terminus of FEN-1; FEN-1 stimulates NEIL1 activity up to 5-fold on multiple DNA substrates; the interaction is required for efficient NEIL1-initiated long-patch BER. Co-immunoprecipitation from human cells; in vitro binding (Kd measurement); in vitro activity stimulation; domain-mapping; co-localization; in vitro LP-BER reconstitution The Journal of biological chemistry High 18662981
2008 Downregulation of NEIL1 by antisense oligonucleotides increases spontaneous mutation frequency ~3-fold at the Hprt locus (predominantly at A:T base pairs) and enhances mutation frequency ~7–8-fold under oxidative stress, demonstrating NEIL1 prevents mutagenesis at endogenous oxidized A and T lesions. Antisense oligonucleotide knockdown; Hprt mutation frequency assay; oxidative damage quantification DNA repair Medium 18495559
2009 NEIL1 excises the unhooked psoralen-ICL fragment from a three-stranded DNA structure via glycosidic bond hydrolysis; complete short-patch BER reconstitution of the unhooked ICL was demonstrated in vitro, placing NEIL1 downstream of XPF/ERCC1 and translesion synthesis in ICL repair. In vitro DNA glycosylase assay on three-stranded crosslinked substrates; BER reconstitution assay The Journal of biological chemistry High 19258314
2009 CSB physically interacts with NEIL1 (co-immunoprecipitation, co-localization in HeLa cells) and stimulates NEIL1 incision activity in vitro; combined knockdown of CSB and NEIL1 strongly inhibits repair of FapyGua in cells; csb-/- mice accumulate higher endogenous FapyAde and FapyGua levels. Co-immunoprecipitation; co-localization; in vitro stimulation assay; shRNA double-knockdown; lesion quantification by mass spectrometry in knockout mice The Journal of biological chemistry High 19179336
2010 RNA editing by ADAR1 converts lysine 242 to arginine in the lesion recognition loop of NEIL1, altering substrate specificity: the edited form removes thymine glycol from duplex DNA ~30-fold more slowly, while editing enhances repair of guanidinohydantoin. ADAR1 is the responsible editing enzyme acting on an A-C mismatch in an exon 6 / intron 5 hairpin. In vitro DNA glycosylase kinetic assay comparing edited vs unedited NEIL1; identification of editing enzyme by siRNA; interferon-alpha treatment of human cells Proceedings of the National Academy of Sciences of the United States of America High 21068368
2010 NEIL1 interacts with RPA (large subunit, KD ~20 nM) via NEIL1 C-terminal residues 312–349; RPA is present in the NEIL1 immunocomplex and increases after oxidative stress; RPA inhibits NEIL1 base excision activity on ssDNA at the replication fork, but this inhibition is relieved by PCNA. Co-immunoprecipitation; in vitro binding (Kd measurement); in vitro activity assay; domain-mapping by deletion mutants DNA repair High 20338831
2010 NEIL1 can efficiently remove 8-oxo-7,8-dihydroadenine (8-oxoAde) from 8-oxoAde:Cyt pairs in duplex DNA; in an in vitro reconstituted system NEIL1 causes abortive repair initiation (stops after base removal and strand cleavage), which is partially relieved by polynucleotide kinase/phosphatase. In vitro glycosylase assay; in vitro BER reconstitution FEBS letters Medium 20214901
2010 Genetic evidence (accumulation of R-cdA and S-cdA in neil1-/- mouse liver DNA but not ogg1-/- mice) suggests NEIL1 participates in nucleotide excision repair of 8,5'-cyclopurine lesions that cannot be repaired by BER, expanding NEIL1's repair repertoire beyond BER. Neil1-/- mouse knockout; lesion quantification by isotope-dilution GC-MS/MS Biochemistry Medium 20067321
2012 NEIL1 physically interacts with PARP-1: PARP-1 binds the C-terminal ~100 aa of NEIL1 and NEIL1 binds the BRCT domain of PARP-1; NEIL1 stimulates poly(ADP-ribosyl)ation activity of PARP-1 in vitro; PARP-1 inhibits NEIL1 incision activity in a concentration-dependent manner; NEIL1-deficient fibroblasts have impaired PAR formation after DNA damage, rescued by NEIL1 re-expression. In vitro binding assay; Co-immunoprecipitation; in vitro PAR activity assay; domain-mapping; cell-based rescue experiment Aging Medium 23104860
2012 hnRNP-U directly interacts with NEIL1 via the C-terminal interaction domain (KD ~54 nM); hnRNP-U stimulates NEIL1 base excision activity on multiple DNA substrates by enhancing product release; depletion of hnRNP-U and NEIL1 epistatically sensitizes cells to low oxidative damage. Co-immunoprecipitation; in vitro binding (Kd); in vitro stimulation assay; siRNA epistasis; cell survival assay; CD spectroscopy The Journal of biological chemistry High 22902625
2012 Neil1 acts as a genetic modifier of CAG trinucleotide repeat (TNR) expansion; Neil1-/- mice show significantly reduced somatic and germline TNR expansions in R6/1 Huntington's disease model mice; purified NEIL1 binds and excises 5-hydroxycytosine from duplex DNA more efficiently than from hairpin substrates, suggesting NEIL1-initiated BER can trigger repeat expansion. Neil1-/- knockout mouse genetics; repeat expansion assay (mean repeat change, instability index); in vitro glycosylase activity on hairpin vs duplex substrates Human molecular genetics High 22914735
2012 NEIL1 excises oxidized guanine lesions Gh and Sp from quadruplex DNA with enhanced glycosylase activity on Gh in the telomeric sequence context; NEIL3 (not NEIL1) has activity on Tg in quadruplex DNA. In vitro DNA glycosylase assay on quadruplex DNA substrates containing defined lesions; comparison of five mammalian glycosylases The Journal of biological chemistry Medium 23926102
2012 NEIL1 biochemical mapping revealed that Pro2 and Lys54 are both involved in AP lyase activity (Schiff-base formation); residues Met81, Arg119, and Phe120 are essential for 8-oxoG removal; arginine and histidine residues in the zincless finger loop are required for lesion processing; the disordered C-terminal region harbors the interaction interface for partner proteins. Site-directed mutagenesis; in vitro DNA glycosylase/AP lyase assays; single-turnover kinetics; Schiff-base trapping DNA repair High 22858590
2013 NEIL1 is present in the DNA replication complex from human cells; NEIL1 associates with replication foci during S-phase and co-localizes with RPA-coated ssDNA template, inhibiting DNA polymerase delta synthesis upon encountering an oxidized base; NEIL1 deficiency slows nascent chain growth under oxidative stress; NEIL1 interacts with replication proteins RFC, pol delta, and DNA ligase I via its C-terminal domain. Gel filtration of nuclear extracts; co-immunoprecipitation; co-localization with BrdU/RPA foci; iPOND-like nascent chain analysis; in vitro primer extension inhibition; siRNA knockdown + BrdU incorporation Proceedings of the National Academy of Sciences of the United States of America High 23898192
2013 The disordered C-terminal domain (CTD) of NEIL1 interacts intramolecularly with the folded domain, stabilizing the native protein structure; a Lys-rich cluster (residues 355–360) contributes to this electrostatic stabilization; SAXS confirms CTD flexibility; deletion of CTD reduces protein stability. Intrinsic fluorescence spectroscopy; SAXS; circular dichroism; stability assays with osmolytes; mutagenesis of Lys cluster Journal of molecular biology Medium 23542007
2015 The C-terminal domain (CTD) of NEIL1 is required for BERosome complex formation with replication proteins (RFC, pol delta, DNA ligase I); NEIL1 lacking the CTD does not associate with chromatin or replication foci during S phase; CTD polypeptide acts as a dominant-negative inhibitor in vitro and sensitizes cells to ROS when ectopically expressed; RFC stimulates NEIL1 activity ~8-fold. Co-immunoprecipitation; chromatin fractionation; replication foci co-localization; in vitro BER reconstitution; dominant-negative expression; cell survival after ROS The Journal of biological chemistry High 26134572
2016 NEIL1 is polyubiquitylated by two E3 ubiquitin ligases, Mule and TRIM26, at C-terminal lysine residues; knockdown of either ligase stabilizes NEIL1 protein; ionizing radiation induces NEIL1 protein specifically in a Mule-dependent manner; stabilization of NEIL1 via TRIM26 knockdown increases cellular resistance to ionising radiation. Purification of E3 ligases from human cells; in vitro ubiquitylation assay; siRNA knockdown; protein stability assay; clonogenic survival after IR Nucleic acids research High 27924031
2017 NEIL1 efficiently recognizes and excises the AFB1-Fapy-dG adduct from DNA in vitro; Neil1-/- mice show increased AFB1-Fapy-dG levels in liver DNA and are highly susceptible to AFB1-induced hepatocellular carcinoma (greater than XPA-/- mice deficient in NER). In vitro DNA glycosylase assay; AFB1-Fapy-dG quantification by mass spectrometry in neil1-/- mouse livers; carcinogenesis bioassay (tumor frequency and size) in neil1-/- vs WT vs xpa-/- mice Proceedings of the National Academy of Sciences of the United States of America High 28373545
2017 NEIL1 directly excises 5-carboxylcytosine (5caC) from dsDNA substrates; NEIL1 also stimulates TDG glycosylase activity on 5-formylcytosine (5fC) and 5caC substrates; NEIL1 provides AP lyase activity downstream of TDG glycosylase activity. Catalytically impaired NEIL1 P2T and E3Q mutants were used as controls. In vitro glycosylase assay with purified NEIL1 and catalytic mutants; in vitro reconstitution with TDG; substrate-specific activity assays on modified cytosine-containing dsDNA Scientific reports Medium 28827588
2017 Human NEIL3 (and NEIL1) cleave psoralen-induced interstrand crosslinks in four-stranded DNA structures; NEIL1 cleaves to generate two unhooked duplexes with a nick, whereas NEIL3 targets both DNA strands without generating single-strand breaks. In vitro DNA glycosylase assay on three-stranded and four-stranded psoralen-crosslinked substrates; product characterization Scientific reports Medium 29234069
2017 NEIL1 destabilizes the PCNA homotrimer upon interaction; SEC, native gel electrophoresis, mass spectrometry, AFM, and SAXS together demonstrate formation of a 1:1:1 NEIL1-DNA-PCNA(monomer) complex, suggesting a mechanistic switch between replication and BER. Size exclusion chromatography; native gel electrophoresis; mass spectrometry; atomic force microscopy; SAXS; ab initio shape reconstruction Nucleic acids research High 27994037
2018 NEIL1 is acetylated by histone acetyltransferase p300 predominantly at Lys 296, 297, and 298 in its C-terminal domain; acetylation is required for nuclear localization and chromatin association (non-acetylable 3KA mutant loses nuclear localization); acetylated NEIL1 is exclusively chromatin-bound; acetylation enhances glycosylase activity via increased product release; acetylation-defective cells show reduced BER complex formation and greater sensitivity to oxidative stress. In vitro acetylation assay; site-directed mutagenesis; subcellular fractionation; immunofluorescence; in vitro glycosylase activity; BER complex activity; cell survival assay DNA repair High 29698889
2018 NEIL1 is present in purified mitochondrial extracts; NEIL1 interacts with mitochondrial single-stranded DNA binding protein (mtSSB) via its C-terminal tail; in absence of DNA, NEIL1 disrupts the mtSSB homotetramer forming a smaller NEIL1-mtSSB monomer complex; in presence of DNA, a larger ternary complex forms. Mitochondrial fractionation; protein painting; far-western analysis; gel-filtration chromatography; MALS; SAXS DNA repair Medium 29522991
2016 JNK1 kinase interacts with NEIL1 in vitro and in immunoprecipitates, and phosphorylates NEIL1 at Ser207, Ser306, and Ser61; phosphomimetic mutation of Y263 yields a completely inactive enzyme; phosphorylation at S207, S306, and S61 does not affect DNA binding or enzyme activity. Mass spectrometry identification of phosphorylation sites; co-immunoprecipitation; in vitro kinase assay; mutagenesis plus activity assay PloS one Medium 27518429
2019 NEIL1 and NEIL2 protect mitochondrial DNA from oxidative damage during neural crest development; Neil-deficiency in Xenopus embryos and mouse ESCs causes a TP53-dependent DNA damage response and intrinsic apoptosis specifically in cranial neural crest cells; epistasis experiments rule out involvement of epigenetic DNA demethylation. Xenopus morpholino knockdown; mouse ESC differentiation; Neil1/2 knockout; TP53 epistasis; mitochondrial DNA damage measurement; apoptosis assay eLife High 31566562
2019 NEIL1 preferentially excises oxidized bases from double-stranded DNA including damage upstream of a replication fork, whereas NEIL3 preferentially excises from ssDNA and open fork structures; the two glycosylases act in concert at the replication fork. In vitro glycosylase assay on model replication fork substrates with site-specific lesions at defined positions relative to the fork Genes Medium 31018584
2019 NEIL1 kinetics on AFB1-Fapy-dG and NM-Fapy-dG adducts: unedited NEIL1 (K242) has ~1.4-fold higher rate than edited K242R on AFB1-Fapy-dG; the differential is large for ThyGly excision (7.5–12-fold) and 5-OHC (~3–5-fold); both forms preferentially release FapyAde and FapyGua from gamma-irradiated DNA. In vitro glycosylase assay; GC-MS/MS quantification of released lesions; molecular modeling DNA repair Medium 31733589
2022 NEIL1 interacts with mitochondrial transcription factor A (TFAM) via both its N- and C-terminal domains; the interaction is transient and enhanced by the presence of DNA; NEIL1 disrupts TFAM-DNA complex interactions; TFAM-transcribed mitochondrial gene expression is reduced in the absence of NEIL1 under DNA damage conditions. Biochemical co-precipitation; hydrogen-deuterium exchange mass spectrometry; MALS; gel filtration; mRNA expression analysis in NEIL1-deficient cells Frontiers in cell and developmental biology Medium 35938152
2024 Neil1-/- mice challenged with AFB1 show significantly increased mutation frequencies in nuclear (but not mitochondrial) liver genomes; mutation spectra in neil1-/- mice match AFB1-specific COSMIC signature SBS24, confirming NEIL1 limits AFB1-induced mutagenesis and carcinogenesis in vivo. Neil1-/- knockout mice; AFB1 challenge; duplex sequencing of liver DNA; tumor bioassay NAR molecular medicine High 38779538

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2003 Repair of oxidized bases in DNA bubble structures by human DNA glycosylases NEIL1 and NEIL2. The Journal of biological chemistry 294 14522990
2006 The metabolic syndrome resulting from a knockout of the NEIL1 DNA glycosylase. Proceedings of the National Academy of Sciences of the United States of America 211 16446448
2005 Repair of formamidopyrimidines in DNA involves different glycosylases: role of the OGG1, NTH1, and NEIL1 enzymes. The Journal of biological chemistry 174 16221681
2003 The novel DNA glycosylase, NEIL1, protects mammalian cells from radiation-mediated cell death. DNA repair 159 12713815
2005 Recognition of the oxidized lesions spiroiminodihydantoin and guanidinohydantoin in DNA by the mammalian base excision repair glycosylases NEIL1 and NEIL2. DNA repair 141 15533836
2010 RNA editing changes the lesion specificity for the DNA repair enzyme NEIL1. Proceedings of the National Academy of Sciences of the United States of America 136 21068368
2007 Interaction of the human DNA glycosylase NEIL1 with proliferating cell nuclear antigen. The potential for replication-associated repair of oxidized bases in mammalian genomes. The Journal of biological chemistry 128 18032376
2004 The crystal structure of human endonuclease VIII-like 1 (NEIL1) reveals a zincless finger motif required for glycosylase activity. Proceedings of the National Academy of Sciences of the United States of America 126 15232006
2013 Neil3 and NEIL1 DNA glycosylases remove oxidative damages from quadruplex DNA and exhibit preferences for lesions in the telomeric sequence context. The Journal of biological chemistry 114 23926102
2010 Hepatitis C virus induces oxidative stress, DNA damage and modulates the DNA repair enzyme NEIL1. Journal of gastroenterology and hepatology 113 20074151
2007 The human Werner syndrome protein stimulates repair of oxidative DNA base damage by the DNA glycosylase NEIL1. The Journal of biological chemistry 100 17611195
2009 Targeted deletion of the genes encoding NTH1 and NEIL1 DNA N-glycosylases reveals the existence of novel carcinogenic oxidative damage to DNA. DNA repair 97 19346169
2013 Prereplicative repair of oxidized bases in the human genome is mediated by NEIL1 DNA glycosylase together with replication proteins. Proceedings of the National Academy of Sciences of the United States of America 91 23898192
2009 Cockayne syndrome group B protein stimulates repair of formamidopyrimidines by NEIL1 DNA glycosylase. The Journal of biological chemistry 88 19179336
2008 Physical and functional interaction between human oxidized base-specific DNA glycosylase NEIL1 and flap endonuclease 1. The Journal of biological chemistry 87 18662981
2004 Inactivating mutations of the human base excision repair gene NEIL1 in gastric cancer. Carcinogenesis 83 15319300
2010 Mutation versus repair: NEIL1 removal of hydantoin lesions in single-stranded, bulge, bubble, and duplex DNA contexts. Biochemistry 79 20099873
2004 Mouse NEIL1 protein is specific for excision of 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 4,6-diamino-5-formamidopyrimidine from oxidatively damaged DNA. Biochemistry 79 15595846
2012 Endonuclease VIII-like 1 (NEIL1) promotes short-term spatial memory retention and protects from ischemic stroke-induced brain dysfunction and death in mice. Proceedings of the National Academy of Sciences of the United States of America 70 22927410
2007 Human polymorphic variants of the NEIL1 DNA glycosylase. The Journal of biological chemistry 69 17389588
2006 Evaluation of NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 genes in familial colorectal cancer predisposition. BMC cancer 68 17029639
2012 Neil1 is a genetic modifier of somatic and germline CAG trinucleotide repeat instability in R6/1 mice. Human molecular genetics 65 22914735
2007 The human checkpoint sensor Rad9-Rad1-Hus1 interacts with and stimulates NEIL1 glycosylase. Nucleic acids research 65 17395641
2005 Induction of the human oxidized base-specific DNA glycosylase NEIL1 by reactive oxygen species. The Journal of biological chemistry 61 16118226
2004 Stimulation of DNA glycosylase activity of OGG1 by NEIL1: functional collaboration between two human DNA glycosylases. Biochemistry 60 15350146
2005 NEIL1 excises 3' end proximal oxidative DNA lesions resistant to cleavage by NTH1 and OGG1. Nucleic acids research 59 16129732
2017 NEIL1 protects against aflatoxin-induced hepatocellular carcinoma in mice. Proceedings of the National Academy of Sciences of the United States of America 56 28373545
2011 Exome sequencing identified MYO1E and NEIL1 as candidate genes for human autosomal recessive steroid-resistant nephrotic syndrome. Kidney international 54 21697813
2010 RPA physically interacts with the human DNA glycosylase NEIL1 to regulate excision of oxidative DNA base damage in primer-template structures. DNA repair 52 20338831
2009 The human oxidative DNA glycosylase NEIL1 excises psoralen-induced interstrand DNA cross-links in a three-stranded DNA structure. The Journal of biological chemistry 52 19258314
2010 Evidence for the involvement of DNA repair enzyme NEIL1 in nucleotide excision repair of (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines. Biochemistry 50 20067321
2012 Enhancement of NEIL1 protein-initiated oxidized DNA base excision repair by heterogeneous nuclear ribonucleoprotein U (hnRNP-U) via direct interaction. The Journal of biological chemistry 49 22902625
2011 Variable penetrance of metabolic phenotypes and development of high-fat diet-induced adiposity in NEIL1-deficient mice. American journal of physiology. Endocrinology and metabolism 48 21285402
2011 Structural characterization of viral ortholog of human DNA glycosylase NEIL1 bound to thymine glycol or 5-hydroxyuracil-containing DNA. The Journal of biological chemistry 48 22170059
2009 Non-specific DNA binding interferes with the efficient excision of oxidative lesions from chromatin by the human DNA glycosylase, NEIL1. DNA repair 47 20005182
2016 Abnormal Expressions of DNA Glycosylase Genes NEIL1, NEIL2, and NEIL3 Are Associated with Somatic Mutation Loads in Human Cancer. Oxidative medicine and cellular longevity 46 27042257
2015 The C-terminal Domain (CTD) of Human DNA Glycosylase NEIL1 Is Required for Forming BERosome Repair Complex with DNA Replication Proteins at the Replicating Genome: DOMINANT NEGATIVE FUNCTION OF THE CTD. The Journal of biological chemistry 45 26134572
2013 The disordered C-terminal domain of human DNA glycosylase NEIL1 contributes to its stability via intramolecular interactions. Journal of molecular biology 43 23542007
2009 Catalytically impaired hMYH and NEIL1 mutant proteins identified in patients with primary sclerosing cholangitis and cholangiocarcinoma. Carcinogenesis 41 19443904
2013 Genome and cancer single nucleotide polymorphisms of the human NEIL1 DNA glycosylase: activity, structure, and the effect of editing. DNA repair 39 24382305
2012 Coordination of DNA repair by NEIL1 and PARP-1: a possible link to aging. Aging 39 23104860
2009 Structural characterization of a viral NEIL1 ortholog unliganded and bound to abasic site-containing DNA. The Journal of biological chemistry 39 19625256
2019 The Biochemical Role of the Human NEIL1 and NEIL3 DNA Glycosylases on Model DNA Replication Forks. Genes 38 31018584
2012 Biochemical mapping of human NEIL1 DNA glycosylase and AP lyase activities. DNA repair 37 22858590
2006 Purification and characterization of NEIL1 and NEIL2, members of a distinct family of mammalian DNA glycosylases for repair of oxidized bases. Methods in enzymology 37 16793361
2012 Epigenetic screen of human DNA repair genes identifies aberrant promoter methylation of NEIL1 in head and neck squamous cell carcinoma. Oncogene 36 22286769
2012 Human DNA Glycosylase NEIL1's Interactions with Downstream Repair Proteins Is Critical for Efficient Repair of Oxidized DNA Base Damage and Enhanced Cell Survival. Biomolecules 35 23926464
2017 The Human DNA glycosylases NEIL1 and NEIL3 Excise Psoralen-Induced DNA-DNA Cross-Links in a Four-Stranded DNA Structure. Scientific reports 34 29234069
2016 Ubiquitylation-dependent regulation of NEIL1 by Mule and TRIM26 is required for the cellular DNA damage response. Nucleic acids research 33 27924031
2010 The role of mammalian NEIL1 protein in the repair of 8-oxo-7,8-dihydroadenine in DNA. FEBS letters 33 20214901
2019 NEIL1 and NEIL2 DNA glycosylases protect neural crest development against mitochondrial oxidative stress. eLife 32 31566562
2008 Functional variants of the NEIL1 and NEIL2 genes and risk and progression of squamous cell carcinoma of the oral cavity and oropharynx. Clinical cancer research : an official journal of the American Association for Cancer Research 31 18594018
2019 Recognition of DNA adducts by edited and unedited forms of DNA glycosylase NEIL1. DNA repair 30 31733589
2017 Nei-like 1 (NEIL1) excises 5-carboxylcytosine directly and stimulates TDG-mediated 5-formyl and 5-carboxylcytosine excision. Scientific reports 30 28827588
2019 NEIL1 stimulates neurogenesis and suppresses neuroinflammation after stress. Free radical biology & medicine 27 31175982
2009 Deficiency of the oxidative damage-specific DNA glycosylase NEIL1 leads to reduced germinal center B cell expansion. DNA repair 27 19782007
2008 Mutator phenotype of mammalian cells due to deficiency of NEIL1 DNA glycosylase, an oxidized base-specific repair enzyme. DNA repair 27 18495559
2017 Monitoring of the spatial and temporal dynamics of BER/SSBR pathway proteins, including MYH, UNG2, MPG, NTH1 and NEIL1-3, during DNA replication. Nucleic acids research 26 28575236
2015 Association between single nucleotide polymorphisms of MUTYH, hOGG1 and NEIL1 genes, and depression. Journal of affective disorders 26 26074017
2013 Identification and quantification of human DNA repair protein NEIL1 by liquid chromatography/isotope-dilution tandem mass spectrometry. Journal of proteome research 26 23268652
2013 A NEIL1 single nucleotide polymorphism (rs4462560) predicts the risk of radiation-induced toxicities in esophageal cancer patients treated with definitive radiotherapy. Cancer 25 24022861
2017 Pre-Replicative Repair of Oxidized Bases Maintains Fidelity in Mammalian Genomes: The Cowcatcher Role of NEIL1 DNA Glycosylase. Genes 23 28665322
2017 The NEIL1 G83D germline DNA glycosylase variant induces genomic instability and cellular transformation. Oncotarget 23 29156764
2021 NEIL1 and NEIL2 Are Recruited as Potential Backup for OGG1 upon OGG1 Depletion or Inhibition by TH5487. International journal of molecular sciences 22 33925271
2018 The C-terminal tail of the NEIL1 DNA glycosylase interacts with the human mitochondrial single-stranded DNA binding protein. DNA repair 22 29522991
2018 Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress. DNA repair 22 29698889
2010 Phenotypic enhancement of thymidylate synthetase pathway inhibitors following ablation of Neil1 DNA glycosylase/lyase. Cell cycle (Georgetown, Tex.) 22 21131780
2013 NEIL1 responds and binds to psoralen-induced DNA interstrand crosslinks. The Journal of biological chemistry 21 23508956
2007 NEIL1 is the major DNA glycosylase that processes 5-hydroxyuracil in the proximity of a DNA single-strand break. Biochemistry 21 17348689
2016 Association between Single-Nucleotide Polymorphisms of the hOGG1,NEIL1,APEX1, FEN1,LIG1, and LIG3 Genes and Alzheimer's Disease Risk. Neuropsychobiology 20 27010693
2012 Effect of the multifunctional proteins RPA, YB-1, and XPC repair factor on AP site cleavage by DNA glycosylase NEIL1. Journal of molecular recognition : JMR 20 22434712
2014 Loss of NEIL1 causes defects in olfactory function in mice. Neurobiology of aging 19 25448603
2019 Characterization of rare NEIL1 variants found in East Asian populations. DNA repair 18 31100703
2018 Processing of N5-substituted formamidopyrimidine DNA adducts by DNA glycosylases NEIL1 and NEIL3. DNA repair 18 30448017
2019 Sirt3 regulates the level of mitochondrial DNA repair activity through deacetylation of NEIL1, NEIL2, OGG1, MUTYH, APE1 and LIG3 in colorectal cancer. Polski przeglad chirurgiczny 17 32312920
2016 Phosphorylation Sites Identified in the NEIL1 DNA Glycosylase Are Potential Targets for the JNK1 Kinase. PloS one 16 27518429
2024 NEIL1 drives the initiation of colorectal cancer through transcriptional regulation of COL17A1. Cell reports 15 38175757
2021 DNA Sequence Modulates the Efficiency of NEIL1-Catalyzed Excision of the Aflatoxin B1-Induced Formamidopyrimidine Guanine Adduct. Chemical research in toxicology 15 33595290
2017 Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase. Nucleic acids research 15 27994037
2015 Regulation of NEIL1 protein abundance by RAD9 is important for efficient base excision repair. Nucleic acids research 15 25873625
2012 A DNA oligomer containing 2,2,4-triamino-5(2H)-oxazolone is incised by human NEIL1 and NTH1. Mutation research 15 22465744
2010 Substrate specific stimulation of NEIL1 by WRN but not the other human RecQ helicases. DNA repair 15 20346739
2022 NEIL1 Recoding due to RNA Editing Impacts Lesion-Specific Recognition and Excision. Journal of the American Chemical Society 14 35917336
2020 Evaluation of the influence of chronic low-dose radiation on DNA repair gene polymorphisms [XRCC1, XRCC3, PRKDC (XRCC7), LIG1, NEIL1] in individuals from normal and high level natural radiation areas of Kerala Coast. International journal of radiation biology 14 32149571
2019 Conformational Dynamics of Damage Processing by Human DNA Glycosylase NEIL1. Journal of molecular biology 14 30716333
2016 Enhanced sensitivity of Neil1-/- mice to chronic UVB exposure. DNA repair 14 27818081
2021 NEIL1 and NEIL2 DNA glycosylases modulate anxiety and learning in a cooperative manner in mice. Communications biology 13 34857879
2010 Three novel NEIL1 promoter polymorphisms in gastric cancer patients. World journal of gastrointestinal oncology 13 21160930
2012 NEIL1 binding to DNA containing 2'-fluorothymidine glycol stereoisomers and the effect of editing. Chembiochem : a European journal of chemical biology 12 22639086
2022 Complex Roles of NEIL1 and OGG1: Insights Gained from Murine Knockouts and Human Polymorphic Variants. DNA 10 41059221
2023 Functional analyses of single nucleotide polymorphic variants of the DNA glycosylase NEIL1 in sub-Saharan African populations. DNA repair 9 37517321
2022 Novel interaction interfaces mediate the interaction between the NEIL1 DNA glycosylase and mitochondrial transcription factor A. Frontiers in cell and developmental biology 9 35938152
2020 Nei Endonuclease VIII-Like1 (NEIL1) Inhibits Apoptosis of Human Colorectal Cancer Cells. BioMed research international 9 32685496
2015 Effects of Turkish propolis on expression of hOGG-1 and NEIL-1. Turkish journal of medical sciences 9 26422850
2010 Induction of NEIL1 and NEIL2 DNA glycosylases in aniline-induced splenic toxicity. Toxicology and applied pharmacology 9 21145906
2025 METTL14-mediated depression of NEIL1 aggravates oxidative damage and mitochondrial dysfunction of lens epithelial cells through regulating KEAP1/NRF2 pathways. Cellular signalling 8 39855533
2015 NEIL1 p.Gln282Stop variant is predominantly localized in the cytoplasm and exhibits reduced activity in suppressing mutations. Gene 8 26095805
2025 Role of NEIL1 in genome maintenance. DNA repair 7 40010204
2024 Frequencies and spectra of aflatoxin B1-induced mutations in liver genomes of NEIL1-deficient mice as revealed by duplex sequencing. NAR molecular medicine 7 38779538
2021 RNA Editing of the Human DNA Glycosylase NEIL1 Alters Its Removal of 5-Hydroxyuracil Lesions in DNA. Biochemistry 7 33929180

Missed literature

Know a paper Affinage missed for NEIL1? Flag it for the maintainers and the community.

No submissions yet.