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Showing NECTIN3 — NECTIN-3 is a alias.

NECTIN3

Nectin-3 · UniProt Q9NQS3

Length
549 aa
Mass
61.0 kDa
Annotated
2026-06-10
43 papers in source corpus 27 papers cited in narrative 27 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NECTIN3 is a Ca2+-independent immunoglobulin-like cell adhesion molecule that organizes cell-cell junctions across epithelial, germ cell, and neuronal tissues by forming cis-homodimers and engaging trans-homophilic and trans-heterophilic contacts through its membrane-distal V domain (PMID:10744716, PMID:12011057). Its heterophilic partnerships are functionally specialized: high-affinity V-to-V trans-interaction with nectin-1 mediates apex-apex adhesion of ciliary epithelia (PMID:12011057, PMID:15728677), trans-interaction with nectin-2 builds Sertoli-spermatid junctions required for spermatid development and lymphocyte transendothelial migration (PMID:16923130, PMID:24116228), and heterophilic engagement with Necl-5/CD155 recruits afadin, E-cadherin, and catenins to nucleate adherens junctions (PMID:12759359, PMID:15330856). The cytoplasmic C-terminal class II PDZ-binding motif (EXYV) binds the afadin PDZ domain — the terminal residues are sufficient for binding and widen the PDZ groove — linking nectin-3 to the actin cytoskeleton (PMID:11024295, PMID:25534554). Junctional retention versus removal of nectin-3 is dynamically regulated: FAK (Tyr397)-dependent phosphorylation controls retention at the apical ectoplasmic specialization (PMID:23073828), clathrin-dependent endocytosis triggered by Necl-5 interaction down-regulates surface levels to enforce contact inhibition (PMID:16216929), and MMP-9 proteolytically cleaves perisynaptic nectin-3 (PMID:25232752). Through these mechanisms nectin-3 enforces correct junctional geometry and polarity protein localization in hair cells and cochlear supporting cells downstream of PCP/Vangl2-Rac1 signaling (PMID:24381198, PMID:40207531). In the hippocampus and prefrontal cortex, nectin-3 acts downstream of CRH-CRHR1 signaling to control dendritic spine density, adult neurogenesis, and memory, with its afadin-binding domain required for spine regulation (PMID:23644483, PMID:28872640, PMID:33160402, PMID:37747247). Nectin-3 also serves as a brush-border receptor for Clostridioides difficile toxin B on colonic epithelium (PMID:37747247).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 2000 High

    Establishing nectin-3 as an adhesion molecule required defining how it self-associates and partners with other nectins; this showed it drives homophilic adhesion and links to the cytoskeleton via afadin.

    Evidence Cell adhesion assays, reciprocal Co-IP, and immunofluorescence colocalization across nectin family members

    PMID:10744716 PMID:11024295

    Open questions at the time
    • Structural basis of trans-interaction not resolved
    • Quantitative affinities of homo- vs hetero-interaction not measured at this stage
  2. 2002 High

    It was unknown which structural elements drive nectin-3/nectin-1 binding; domain-swap and SPR work mapped a high-affinity (~1 nM) V-to-V interface with C-domain affinity contributions.

    Evidence Surface plasmon resonance, chimeric nectin1/PVR domain-swaps, and antibody/glycoprotein D competition

    PMID:12011057

    Open questions at the time
    • Co-crystal structure of the trans-dimer not determined
    • Whether the same interface governs nectin-2 and Necl-5 binding not tested
  3. 2003 Medium

    Defining the partner repertoire beyond nectins, nectin-3 was shown to trans-interact heterophilically with Necl-5/CD155 (but not homophilically), promoting cell motility and bridging cell-cell to cell-matrix adhesion.

    Evidence Cell-based ligand binding, motility assays, Co-IP, and integrin microdomain colocalization

    PMID:12740392 PMID:12759359

    Open questions at the time
    • Necl-5 does not bind afadin, leaving its cytoplasmic coupling unclear
    • Direct physical link between nectin and integrin systems inferred from colocalization
  4. 2004 Medium

    The downstream consequence of Necl-5/nectin-3 engagement was unresolved; reconstitution showed afadin, E-cadherin, and catenins are recruited specifically to the nectin-3 side to nucleate adherens junctions.

    Evidence Stable reconstituted cell lines, immunofluorescence, and blocking antibodies; parallel work mapped Necl-5-driven migration to integrin alphaVbeta3/Cdc42/Rac

    PMID:14871893 PMID:15330856

    Open questions at the time
    • Why recruitment is asymmetric to the nectin-3 side mechanistically unexplained
    • Migration arm is nectin-3-independent, so two functions are coupled only at the receptor level
  5. 2005 High

    How nectins regulate contact inhibition was unknown; nectin-3 was shown to trigger clathrin-dependent endocytic down-regulation of Necl-5, reducing movement and proliferation, while in vivo two knockouts established a requirement for nectin-1/nectin-3 apex-apex adhesion in ciliary epithelia.

    Evidence Surface down-regulation assays with clathrin inhibitors; nectin-1-/- and nectin-3-/- mice with immuno-EM

    PMID:15728677 PMID:16216929

    Open questions at the time
    • Adaptor machinery driving clathrin recruitment to nectin-3 not identified
    • Generality of endocytic regulation beyond Necl-5 untested
  6. 2006 High

    The physiological role of nectin-3 heterophilic adhesion was tested genetically; nectin-3-/- mice are male-infertile with disrupted Sertoli-spermatid junctions and mislocalized nectin-2, rescued by wild-type stem cell transplantation.

    Evidence Nectin-3 knockout mice, immunolocalization, spermatogenic stem cell transplantation rescue

    PMID:16923130

    Open questions at the time
    • Cell-autonomous vs non-autonomous contribution only partly resolved by transplantation
    • Signaling consequences of junction loss not defined
  7. 2012 Medium

    Mechanisms controlling junctional retention and new partner contexts were addressed; FAK(Tyr397)/sFRP1 signaling controls nectin-3 retention at the apical ectoplasmic specialization, and CADM1-nectin-3 mediates mast cell-neurite attachment with calcium signaling.

    Evidence In vivo recombinant sFRP1, coculture overexpression with phospho-FAK/phospho-nectin-3 blots; neutralizing-antibody adhesion and calcium imaging

    PMID:22703826 PMID:23073828

    Open questions at the time
    • Direct phosphorylation site on nectin-3 not mapped
    • Whether FAK acts directly on nectin-3 or via intermediates unresolved
  8. 2013 High

    A neuronal function was established; hippocampal nectin-3 is required for stress effects on spatial memory and spine density, with stress reducing nectin-3 via CRH-CRHR1 (not glucocorticoid receptor) signaling, and restoration rescuing early-life stress deficits.

    Evidence AAV knockdown/overexpression, CRHR1 KO and CRH-transgenic mice, behavioral and spine analyses; parallel nectin-2/nectin-3 blockade inhibited lymphocyte transendothelial migration

    PMID:23644483 PMID:24116228

    Open questions at the time
    • Molecular link from CRHR1 signaling to nectin-3 transcription/turnover not fully defined
    • Synaptic partner mediating spine effects in vivo not identified
  9. 2014 High

    Post-translational removal of synaptic nectin-3 and its role in tissue patterning were resolved; MMP-9 cleaves perisynaptic CA1 nectin-3 downstream of NMDA receptor activation under chronic stress, and nectin-3 loss causes aberrant hair-cell contacts with polarity protein mislocalization.

    Evidence Gelatinase assays, MMP-9/NMDAR pharmacology, behavior; nectin-3 KO mice with confocal/EM and polarity protein staining

    PMID:24381198 PMID:25232752

    Open questions at the time
    • Cleavage site on nectin-3 not mapped
    • How aberrant adhesion drives polarity protein mislocalization mechanistically unclear
  10. 2015 High

    The structural basis of cytoskeletal coupling and a glycosylation control point were defined; the nectin-3 C-terminal triresidue is sufficient for afadin PDZ binding and widens the groove, while TGFBR2 signaling controls nectin-3 sialylation in colon cancer.

    Evidence X-ray crystallography of AFPDZ-nectin-3 complex; TGFBR2 reconstitution with click-chemistry sialic acid labeling and mass spectrometry

    PMID:25534554 PMID:26177744

    Open questions at the time
    • Functional consequence of nectin-3 sialylation change not established
    • Single-method evidence for the glycosylation finding
  11. 2017 Medium

    The trafficking route for nectin-3 removal and an additional neuronal role were characterized; nectin-3 is internalized via Rab5-associated tubulobulbar complexes at Sertoli-spermatid junctions, and dentate gyrus nectin-3 modulates adult-born neuron maturation and memory.

    Evidence Immuno-EM with Rab5/EEA1; AAV and retroviral newborn-neuron-specific knockdown with neurogenesis markers and behavior

    PMID:28176461 PMID:28872640

    Open questions at the time
    • Endocytic adaptors linking nectin-3 to tubulobulbar complexes not identified
    • Molecular targets coupling nectin-3 to differentiation markers unknown
  12. 2020 Medium

    Transcriptional and circuit-level regulation were extended; ZNF582 directly represses nectin-3 to suppress nasopharyngeal carcinoma invasion, mPFC nectin-3 mediates adolescent stress effects on behavior and spines, and the afadin-binding domain was shown to be required for nectin-3's spine-density control in visual cortex.

    Evidence ChIP-seq/ChIP-qPCR with rescue epistasis; AAV mPFC knockdown with membrane fractionation; in utero electroporation with afadin-binding-deletion constructs

    PMID:32385776 PMID:33038291 PMID:33160402

    Open questions at the time
    • Whether ZNF582 regulation operates outside NPC unknown
    • Direct synaptic mechanism by which afadin coupling shapes spines not resolved
  13. 2023 Medium

    An unexpected functional role at the colonic surface was identified; nectin-3 localizes to the colonocyte brush border and serves as a receptor for Clostridioides difficile toxin B.

    Evidence Immunofluorescence on colonic tissue and receptor localization studies

    PMID:37747247

    Open questions at the time
    • Binding interface between TcdB and nectin-3 not mapped
    • Functional contribution to in vivo toxin entry not quantified
  14. 2024 Medium

    Causal placement of nectin-3 downstream of CRHR1 in memory was strengthened, and a PCP-Rac1 axis controlling its junctional localization in axon guidance was defined.

    Evidence AAV bidirectional manipulation with CRHR1 antagonism (antalarmin); Nectin-3/Rac1 conditional KO epistasis with Vangl2 mutants and junctional protein staining

    PMID:39395912 PMID:40207531

    Open questions at the time
    • Direct molecular signal from CRHR1 to nectin-3 still indirect
    • How PCP signaling positions nectin-3 at the junction mechanistically unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the same V-domain adhesion code is selectively read out into distinct outcomes (junction assembly, endocytic contact inhibition, spine regulation, toxin entry) across tissues remains unresolved.
  • No unified model linking partner choice to downstream signaling output
  • Tissue-specific regulators of nectin-3 turnover incompletely defined
  • Structure of full trans-adhesion complex unsolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098631 cell adhesion mediator activity 4 GO:0060090 molecular adaptor activity 2 GO:0001618 virus receptor activity 1
Localization
GO:0005886 plasma membrane 4 GO:0005856 cytoskeleton 3
Pathway
R-HSA-1266738 Developmental Biology 4 R-HSA-112316 Neuronal System 3 R-HSA-1500931 Cell-Cell communication 3
Partners
AFDNCADM1MMP9NECTIN1NECTIN2PTK2PVR
Complex memberships
adherens junctionnectin-afadin adhesion complex

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 Nectin-3alpha forms a cis-homodimer and undergoes Ca2+-independent trans-homo-interaction to cause homophilic cell-cell adhesion; it also undergoes trans-hetero-interaction with nectin-1 or nectin-2 (but does not form cis-heterodimers with them). Nectin-3alpha co-localizes with nectin-2 at cadherin-based adherens junctions and interacts with afadin, an actin filament-binding protein that connects nectin to the actin cytoskeleton. Cell-based adhesion assays, co-immunoprecipitation, immunofluorescence colocalization The Journal of biological chemistry High 10744716
2000 Nectin-3/PRR3 carries the C-terminal A/EXYV consensus motif and interacts in vivo with both long and short isoforms of afadin via this motif. In vivo co-immunoprecipitation, sequence analysis Gene Medium 11024295
2002 The trans-hetero-interaction of nectin-3 with nectin-1 is mediated through V-to-V domain contacts (KD ~1 nM), with C domains contributing to increased affinity. The minimal nectin-3 binding region on nectin-1 was mapped to the C-C'-C"-D beta-strands of the V domain using chimeric nectin1/PVR receptors. Surface plasmon resonance binding assays, chimeric receptor domain-swap experiments, competition assays with blocking antibodies and HSV-1 glycoprotein D The Journal of biological chemistry High 12011057
2003 Necl-5/Tage4 (later renamed nectin-like molecule-5) heterophilically trans-interacts with nectin-3 but not homophilically, and this trans-interaction enhances motility of V12Ras-NIH3T3 cells. Unlike nectins, Tage4/Necl-5 does not bind afadin. Cell-based ligand binding assays, cell motility assays, co-immunoprecipitation The Journal of biological chemistry Medium 12740392
2003 Nectin-3 binds CD155 (PVR) and its mouse homologue Tage4 in cell-based ligand binding assays. Coculture of nectin-3- and CD155-expressing cells led to CD155-dependent recruitment of nectin-3 to cell-cell contacts. CD155 co-distributes with alpha(v) integrin microdomains, suggesting a trans-interaction between cell-cell (nectin) and cell-matrix (integrin/CD155) adhesion systems at junctions. Cell-based ligand binding assays, immunofluorescence colocalization, heterologous coculture system The Journal of biological chemistry Medium 12759359
2004 Necl-5 enhances cell migration in an integrin alpha(V)beta(3)-dependent, nectin-3-independent manner when cells are not in contact; Necl-5 colocalizes with integrin alpha(V)beta(3) at leading edges. Necl-5-enhanced migration requires activation of Cdc42 and Rac small GTPases. Mutant Necl-5 constructs in L fibroblasts and NIH3T3 cells, integrin inhibitor/activator assays, dominant-negative mutants, small GTPase activation assays The Journal of biological chemistry Medium 14871893
2004 Upon Necl-5/nectin-3 heterophilic trans-interaction, afadin, E-cadherin, and catenins are recruited to the nectin-3 side (not the Necl-5 side) of the contact site, facilitating adherens junction formation. A monoclonal antibody blocking Necl-5/nectin-3 interaction inhibited E-cadherin-based AJ formation. Stable cell lines expressing Necl-5, nectin-3, and E-cadherin; immunofluorescence; blocking antibody experiments Genes to cells Medium 15330856
2005 Nectin-1 and nectin-3 heterophilic trans-interaction is required for establishing apex-apex adhesion between pigment and non-pigment cell layers of the ciliary epithelia; nectin-1-/- and nectin-3-/- mice both show microphthalmia with separation of this contact layer, while apicolateral junctions are unaffected. Genetic knockout mice (nectin-1-/- and nectin-3-/-), immunofluorescence and immunoelectron microscopy Development High 15728677
2005 Upon cell-cell contact, Necl-5 is down-regulated from the cell surface via clathrin-dependent endocytosis initiated by its interaction with nectin-3; this down-regulation reduces cell movement and proliferation, representing a mechanism for contact inhibition. Cell-surface down-regulation assays, clathrin inhibitor experiments, nectin-3-dependent coculture assays The Journal of cell biology High 16216929
2006 Nectin-3 localizes asymmetrically to the spermatid side of Sertoli-spermatid junctions and its heterophilic trans-interaction with nectin-2 (on Sertoli cells) is essential for spermatid development. Nectin-3-/- mice are male-infertile with distorted sperm nuclei, abnormal mitochondrial distribution, and mislocalized nectin-2; wild-type stem cell transplantation partially rescues these defects. Genetic knockout mice (nectin-3-/-), immunolocalization, spermatogenic stem cell transplantation rescue experiment Genes to cells High 16923130
2012 CADM1 on mast cells mediates attachment to dorsal root ganglion (DRG) neurites and subsequent calcium responses via heterophilic binding to nectin-3 expressed on DRG neurons; a neutralizing antibody to nectin-3 inhibited both mast cell attachment and calcium signaling. Neutralizing antibody blocking assay, co-culture attachment assays, calcium imaging Journal of neuroimmunology Medium 22703826
2012 sFRP1 regulates spermatid adhesion and spermiation by suppressing phosphorylation of FAK (Tyr397), which in turn reduces phosphorylation and promotes retention of nectin-3 at the apical ectoplasmic specialization. In vivo administration of recombinant sFRP1 delayed spermiation with concurrent nectin-3 retention; overexpression in Sertoli-germ cell coculture confirmed FAK-nectin-3 phosphorylation axis. In vivo recombinant protein administration, lentiviral overexpression in coculture, Western blotting for phospho-FAK and phospho-nectin-3 FASEB journal Medium 23073828
2013 Hippocampal nectin-3 is necessary for stress effects on spatial memory and dendritic spine density. Acute and chronic stress reduce hippocampal nectin-3 levels via CRH-CRHR1 signaling (not glucocorticoid receptor). Knockdown of hippocampal nectin-3 caused spatial memory deficits and spine loss; restoration of nectin-3 rescued early-life stress effects on memory and spine density in adulthood. AAV-mediated knockdown and overexpression, CRHR1 knockout mice, CRH-overexpressing transgenic mice, fear conditioning and spatial memory tasks, dendritic spine analysis Nature neuroscience High 23644483
2013 Nectin-3 expressed on T lymphocytes trans-interacts with nectin-2 on high endothelial venules; blocking either nectin-3 (on lymphocytes) or nectin-2 (on ECs) with monoclonal antibodies inhibits lymphocyte transendothelial migration in vitro. Soluble nectin-3 binding assays, blocking monoclonal antibodies, transendothelial migration assay PloS one Medium 24116228
2014 MMP-9 proteolytically cleaves nectin-3 in the perisynaptic CA1 region following chronic restraint stress; this process involves NMDA receptor activation and is responsible for stress-induced social and cognitive alterations. Nectin-3 reduction was specific to CA1, not CA3. Gelatinase activity assays, MMP-9 inhibition, NMDA receptor antagonism, immunohistochemistry, behavioral assays Nature communications High 25232752
2014 In Nectin-3-deficient mice, hair cells aberrantly contact each other and form abnormal junctions; this leads to disturbed hair bundle orientation and morphology, abnormal kinocilium positioning, and mislocalization of cadherin-catenin complexes and polarity proteins Pals1 and Par-3 specifically in the aberrantly attached (not unattached) HCs. Nectin-3 knockout mice, immunofluorescence, confocal and electron microscopy Development High 24381198
2015 Crystal structure of the afadin PDZ domain (AFPDZ) in complex with the nectin-3 C-terminal peptide revealed that the last three C-terminal amino acids of nectin-3 (containing two hydrophobic residues of the class II motif) are sufficient for AFPDZ binding, and binding induces a ~2.5 Å-wider ligand-binding groove in AFPDZ compared to unliganded structures. X-ray crystallography of AFPDZ-nectin-3 fusion protein complex Protein science High 25534554
2015 Nectin-3 sialylation is regulated by TGFBR2 signaling in colon cancer cells; upon TGFBR2 reconstitution, nectin-3 loses sialic acid incorporation, identifying nectin-3 as a glycoprotein target of TGFBR2-dependent glycome regulation. RMCE-based TGFBR2 reconstitution, Click-It chemistry for sialic acid labeling, mass spectrometry Protein science Medium 26177744
2016 Nectin-3 is present in rat uterine epithelial cells as three isoforms (80, 60, 32 kDa) and redistributes from basal cytoplasmic localization to cell junctions at the time of implantation (day 6); this junctional localization is regulated by progesterone. At implantation, nectin-3 localizes to the tight junction but does not interact with occludin or l-afadin. Immunofluorescence, Western blotting, co-immunoprecipitation, progesterone manipulation Reproductive sciences Medium 27217376
2017 Nectin-3 protein in spermatids is internalized at tubulobulbar complexes (clathrin/actin-based junction-internalizing structures) at Sertoli-spermatid junctions; nectin-3 labeling occurs at junctions, tubulobulbar complex bulbs, and associated double-membrane vesicles, with Rab5 associating with bulbs before scission. Pre-embedding immunoelectron microscopy with Rab5, EEA1, and nectin-3 antibodies Anatomical record Medium 28176461
2017 AAV-mediated knockdown of nectin-3 in dentate gyrus neurons impaired long-term spatial memory and temporal order memory, increased density of immature doublecortin+ and calretinin+ neurons but decreased calbindin immunoreactivity, indicating nectin-3 modulates differentiation/maturation of adult-born granule cells. Selective nectin-3 knockdown in new DG neurons via retrovirus reduced thin dendritic spines. AAV-mediated knockdown, retroviral labeling of newborn neurons, immunohistochemistry for neurogenesis markers, behavioral testing Translational psychiatry High 28872640
2020 ZNF582 directly regulates transcription of nectin-3 (and NRXN3) as shown by ChIP-seq and ChIP-qPCR; restoration or abrogation of nectin-3 reversed the tumor suppressor effect of ZNF582 on NPC migration and invasion in vitro and in vivo. ChIP-seq, ChIP-qPCR, luciferase reporter assay, rescue/epistasis experiments with nectin-3 overexpression/knockdown Cancer communications Medium 33038291
2020 AAV-mediated knockdown of nectin-3 in mouse medial prefrontal cortex (mPFC) during adolescence reproduced chronic stress-induced impairments in social recognition and spatial working memory, and reduced dendritic complexity and spine density; membrane localization of nectin-3 was significantly correlated with behavioral performance. AAV-mediated knockdown, behavioral testing, dendritic morphology analysis, membrane fractionation Neuroscience bulletin Medium 32385776
2020 Precise levels of nectin-3 in visual cortex layer 2/3 neurons regulate dendritic spine density during synaptogenesis. Knockdown increased dendritic spine density at P21/P35; overexpression decreased it. Overexpression of nectin-3 lacking its afadin-binding domain caused an even greater decrease in spine density on basal dendrites, indicating the afadin-binding domain is important for nectin-3's role in spine formation. In utero electroporation with shRNA knockdown and full-length/truncated overexpression constructs; spine density analysis at multiple developmental timepoints Neural development Medium 33160402
2023 Nectin-3, previously characterized as an adherens junction protein, is also localized to the brush border of colonocytes and can serve as a cell surface receptor for Clostridioides difficile toxin B (TcdB) on colonic epithelial cells. Immunofluorescence microscopy on colonic tissue, receptor localization studies mBio Medium 37747247
2024 Knockdown of dorsal CA1 nectin-3 impaired object recognition memory in adolescent female mice, while overexpression of dorsal CA1 nectin-3 reversed early-life stress-induced recognition memory deficits. Systemic CRHR1 antagonism (antalarmin) upregulated hippocampal nectin-3 levels and rescued memory deficits, placing nectin-3 downstream of CRHR1 signaling. AAV-mediated knockdown and overexpression, pharmacological CRHR1 antagonism, novel object recognition behavioral testing Neuroscience bulletin Medium 39395912
2025 PCP signaling (via Vangl2) regulates the junctional localization of Nectin-3 in cochlear supporting cells; loss of Nectin-3 partially phenocopies SGNII peripheral afferent turning defects seen in Vangl2 mutants. Epistasis analysis indicates Nectin-3 and Rac1 act in the same genetic pathway downstream of PCP to control SGNII axon guidance, with Nectin-3 likely providing a cell adhesion function for afferent turning. Genetic loss-of-function (Nectin-3 and Rac1 conditional knockouts), epistasis with Vangl2 mutants, immunofluorescence of junctional protein localization Development Medium 40207531

Source papers

Stage 0 corpus · 43 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 Nectin-3, a new member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities. The Journal of biological chemistry 247 10744716
2002 Prominent role of the Ig-like V domain in trans-interactions of nectins. Nectin3 and nectin 4 bind to the predicted C-C'-C"-D beta-strands of the nectin1 V domain. The Journal of biological chemistry 118 12011057
2003 Tage4/Nectin-like molecule-5 heterophilically trans-interacts with cell adhesion molecule Nectin-3 and enhances cell migration. The Journal of biological chemistry 114 12740392
2013 Nectin-3 links CRHR1 signaling to stress-induced memory deficits and spine loss. Nature neuroscience 108 23644483
2014 Role for MMP-9 in stress-induced downregulation of nectin-3 in hippocampal CA1 and associated behavioural alterations. Nature communications 107 25232752
2005 Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development. Development (Cambridge, England) 99 15728677
2005 Inhibition of cell movement and proliferation by cell-cell contact-induced interaction of Necl-5 with nectin-3. The Journal of cell biology 90 16216929
2004 Nectin-like molecule-5/Tage4 enhances cell migration in an integrin-dependent, Nectin-3-independent manner. The Journal of biological chemistry 87 14871893
2006 Role of cell adhesion molecule nectin-3 in spermatid development. Genes to cells : devoted to molecular & cellular mechanisms 82 16923130
2003 Recruitment of nectin-3 to cell-cell junctions through trans-heterophilic interaction with CD155, a vitronectin and poliovirus receptor that localizes to alpha(v)beta3 integrin-containing membrane microdomains. The Journal of biological chemistry 71 12759359
2000 Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin. Gene 60 11024295
2017 Nectin-3 modulates the structural plasticity of dentate granule cells and long-term memory. Translational psychiatry 36 28872640
2010 Cooperation of nectin-1 and nectin-3 is required for normal ameloblast function and crown shape development in mouse teeth. Developmental dynamics : an official publication of the American Association of Anatomists 36 21038445
2018 Nectin-3 is a new biomarker that mediates the upregulation of MMP2 and MMP9 in ovarian cancer cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 34 30469078
2013 The expression of the Nectin complex in human breast cancer and the role of Nectin-3 in the control of tight junctions during metastasis. PloS one 32 24386110
2013 Nectin-3 (CD113) interacts with Nectin-2 (CD112) to promote lymphocyte transendothelial migration. PloS one 31 24116228
2020 ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin-3 and NRXN3. Cancer communications (London, England) 30 33038291
2014 Aberrant cochlear hair cell attachments caused by Nectin-3 deficiency result in hair bundle abnormalities. Development (Cambridge, England) 28 24381198
2019 Anti-quorum sensing and antibiofilm activities of Blastobotrys parvus PPR3 against Pseudomonas aeruginosa PAO1. Microbial pathogenesis 25 31644930
2012 Cell adhesion molecule 1 (CADM1) on mast cells promotes interaction with dorsal root ganglion neurites by heterophilic binding to nectin-3. Journal of neuroimmunology 25 22703826
2012 Secreted Frizzled-related protein 1 (sFRP1) regulates spermatid adhesion in the testis via dephosphorylation of focal adhesion kinase and the nectin-3 adhesion protein complex. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24 23073828
2004 Involvement of heterophilic trans-interaction of Necl-5/Tage4/PVR/CD155 with nectin-3 in formation of nectin- and cadherin-based adherens junctions. Genes to cells : devoted to molecular & cellular mechanisms 22 15330856
2017 Loss of ppr3, ppr4, ppr6, or ppr10 perturbs iron homeostasis and leads to apoptotic cell death in Schizosaccharomyces pombe. The FEBS journal 21 27886462
2020 Prefrontal Nectin3 Reduction Mediates Adolescent Stress-Induced Deficits of Social Memory, Spatial Working Memory, and Dendritic Structure in Mice. Neuroscience bulletin 19 32385776
2013 Tauopathy differentially affects cell adhesion molecules in mouse brain: early down-regulation of nectin-3 in stratum lacunosum moleculare. PloS one 19 23704923
2006 Expression of nectin-1, nectin-3, N-cadherin, and NCAM in spinal motoneurons after sciatic nerve transection. Experimental neurology 15 16777094
2015 Crystal structure of afadin PDZ domain-nectin-3 complex shows the structural plasticity of the ligand-binding site. Protein science : a publication of the Protein Society 14 25534554
2017 High Resolution Localization of Rab5, EEA1, and Nectin-3 to Tubulobulbar Complexes in the Rat Testis. Anatomical record (Hoboken, N.J. : 2007) 13 28176461
2019 Postnatal nectin-3 knockdown induces structural abnormalities of hippocampal principal neurons and memory deficits in adult mice. Hippocampus 12 31066147
2015 A new method for detection of tumor driver-dependent changes of protein sialylation in a colon cancer cell line reveals nectin-3 as TGFBR2 target. Protein science : a publication of the Protein Society 12 26177744
2009 Nectin-3 expression is elevated in limbal epithelial side population cells with strongly expressed stem cell markers. Biochemical and biophysical research communications 10 19716807
2023 Nectin-3 and shed forms of CSPG4 can serve as epithelial cell receptors for Clostridioides difficile TcdB. mBio 9 37747247
2020 Precise levels of nectin-3 are required for proper synapse formation in postnatal visual cortex. Neural development 9 33160402
2019 Loss of nectin-3 expression as a marker of tumor aggressiveness in pancreatic neuroendocrine tumor. Pathology international 9 31855317
2016 Nectin-3 Is Increased in the Cell Junctions of the Uterine Epithelium at Implantation. Reproductive sciences (Thousand Oaks, Calif.) 8 27217376
2018 Disruption of ppr3, ppr4, ppr6 or ppr10 induces flocculation and filamentous growth in Schizosaccharomyces pombe. FEMS microbiology letters 7 29878109
2024 Dorsal CA1 NECTIN3 Reduction Mediates Early-Life Stress-Induced Object Recognition Memory Deficits in Adolescent Female Mice. Neuroscience bulletin 4 39395912
2025 Rac1 and Nectin3 are essential for planar cell polarity-directed axon guidance in the peripheral auditory system. Development (Cambridge, England) 2 40207531
2022 Evidence that Nectin-3 is the soybean agglutinin binding protein on rat corneal endothelium cell surfaces. Experimental eye research 2 35964705
2024 Rac1 and Nectin3 are essential for PCP-directed axon guidance in the peripheral auditory system. bioRxiv : the preprint server for biology 1 38895287
2026 Spatiotemporal characterization of a distinct nectin-3+ cell population in the developing and adult mouse retina. Scientific reports 0 42260030
2025 Early-life stress of limited bedding/nesting material induced recognition memory loss and decreased hippocampal VGluT1 and nectin3 levels in aged male mice. Pharmacology, biochemistry, and behavior 0 39987993
2025 The VP5 protein of the oncolytic virus OH2 regulates nectin-3 molecule to modulate tumor cell apoptosis. Virology 0 40446441

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