Affinage

NDUFAB1

Acyl carrier protein, mitochondrial · UniProt O14561

Length
156 aa
Mass
17.4 kDa
Annotated
2026-06-10
100 papers in source corpus 8 papers cited in narrative 8 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NDUFAB1 is the mitochondrial acyl carrier protein (ACP) that functions as a central coordinator of respiratory chain biogenesis, Fe-S cluster assembly, and metabolic state sensing (PMID:31366990, PMID:31473256). Beyond serving as a structural subunit of respiratory Complex I, it coordinates assembly of complexes I, II, and III and supercomplexes by regulating Fe-S cluster biosynthesis and stabilizing Complex I subunits; cardiac-specific deletion causes defective bioenergetics, elevated ROS, and dilated cardiomyopathy, while overexpression enhances bioenergetics (PMID:31366990). NDUFAB1 docks onto LYR-motif proteins LYRM3 (NDUFB9) and LYRM6 (NDUFA6) through the long acyl chain carried on its 4'-phosphopantetheine cofactor, and this acylation is required for assembly into protein complexes (PMID:28802701). Structural studies show that the acyl chain inserts into the hydrophobic core of ISD11 to stabilize it within the NFS1-ISD11-ACP cysteine desulfurase complex that nucleates Fe-S assembly, with the reconstituted ACP-ISD11-NFS1 enzyme further activated by frataxin and ISCU (PMID:28634302, PMID:31664822). Because ACP acylation depends on acetyl-CoA availability, the acylated protein acts as an acetyl-CoA-responsive allosteric activator of LYR proteins, coupling electron transport chain biogenesis to substrate supply (PMID:30118679). NDUFAB1 also supports protein lipoylation and pyruvate dehydrogenase activation via the mitochondrial fatty acid synthesis pathway, linking it to systemic glucose homeostasis and insulin signaling (PMID:31530015). In its unacylated holo form it participates as a component of the mitoribosome (PMID:31473256).

Mechanistic history

Synthesis pass · year-by-year structured walk · 8 steps
  1. 1999 Medium

    Established NDUFAB1 as a nuclear-encoded Complex I subunit bearing an acyl carrier protein signature, defining its molecular identity before its broader roles were known.

    Evidence cDNA cloning, sequence/domain analysis, and mutational screening of Complex I-deficient patient fibroblasts

    PMID:10234612

    Open questions at the time
    • No causative mutation identified, leaving any disease link unresolved
    • Function beyond domain annotation not addressed
    • EF-hand calcium-binding role not functionally characterized
  2. 2017 High

    Resolved how ACP physically engages partner proteins by showing its phosphopantetheine-conjugated acyl chain inserts into the ISD11 hydrophobic core, providing the structural basis for ACP stabilization of the cysteine desulfurase complex.

    Evidence X-ray crystallography and EM of the SDA (NFS1-ISD11-ACP) complex with enzyme kinetics and cell-based validation

    PMID:28634302

    Open questions at the time
    • Did not address how acylation state is regulated
    • Functional consequence for downstream Fe-S cluster output not measured
    • Did not connect SDA role to Complex I assembly
  3. 2017 High

    Demonstrated that ACP docks onto Complex I via LYR-motif subunits LYRM3/LYRM6 and that a long acyl chain on the cofactor is required for this docking, defining the acylation-dependent module assembly principle.

    Evidence Native PAGE, mass spectrometry, biochemical fractionation, and Complex I activity analysis across two Yarrowia lipolytica ACPM paralogs

    PMID:28802701

    Open questions at the time
    • Established in yeast; mammalian module stoichiometry not fully resolved
    • Did not quantify how acyl chain length tunes binding
    • Signaling role of acylation not addressed
  4. 2018 High

    Showed that acetyl-CoA-dependent acylation makes ACP an allosteric activator of LYR proteins, providing a mechanism that couples ETC complex assembly to metabolic substrate availability.

    Evidence In vitro biochemical assays of acetyl-CoA-dependent ACP acylation, ACP-LYR interaction studies, and genetic ETC assembly analyses

    PMID:30118679

    Open questions at the time
    • In vivo dynamics of acetyl-CoA-driven acylation switching not quantified
    • Which LYR proteins are most sensitive to acylation state unclear
    • Link to whole-cell metabolic flux not established
  5. 2019 High

    Defined ACP as a master coordinator of respiratory complex/supercomplex assembly through Fe-S biosynthesis, with cardiac loss causing dilated cardiomyopathy and overexpression protecting against ischemia-reperfusion injury.

    Evidence Cardiac-specific Ndufab1 knockout and overexpression mice with bioenergetics, ROS, and complex/supercomplex assembly assays

    PMID:31366990

    Open questions at the time
    • Tissue-specific to heart; generalizability to other tissues addressed separately
    • Did not separate Fe-S regulatory role from direct subunit role
    • Molecular trigger of ROS elevation not pinpointed
  6. 2019 High

    Connected ACP to systemic metabolism by showing it drives PDH lipoylation via mtFAS and that skeletal-muscle loss disrupts glucose homeostasis and insulin signaling while overexpression protects against diet-induced obesity.

    Evidence Skeletal muscle-specific Ndufab1 knockout and overexpression mice with PDH lipoylation, glucose homeostasis, and complex assembly assays

    PMID:31530015

    Open questions at the time
    • Whether glucose phenotype is secondary to bioenergetic failure not dissected
    • Octanoyl-ACP precursor flux not directly traced
    • Tissue-specific contribution to systemic insulin resistance unresolved
  7. 2019 High

    Provided a high-resolution structure of the human ACP-ISD11 heterodimer and reconstituted an active NFS1 desulfurase, confirming the acyl chain modulates ISD11 foldability and that the core enzyme is activated by frataxin and ISCU.

    Evidence 2.0 Å X-ray crystallography, molecular dynamics, and in vitro NFS1 activity assays with frataxin and ISCU

    PMID:31664822

    Open questions at the time
    • Single lab; in-cell relevance of activation hierarchy not tested
    • Did not link structural state to acetyl-CoA signaling
    • Dynamics of acyl chain exchange in the active complex unresolved
  8. 2019 Medium

    Synthesized the dual-form model in which unacylated ACP joins mitoribosomes while acylated ACP-LYRM heterodimers serve ETC and Fe-S assembly, and proposed acyl-ACPs as metabolic signaling molecules.

    Evidence Review integrating biochemical fractionation, interaction, and metabolic labeling data

    PMID:31473256

    Open questions at the time
    • Signaling-molecule hypothesis not directly demonstrated
    • Functional role of ACP within mitoribosome not established
    • Regulation switching between acylated and unacylated pools unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the acylation state of ACP is dynamically sensed and toggled in vivo to integrate acetyl-CoA flux with respiratory assembly, Fe-S biogenesis, lipoylation, and mitoribosome participation remains to be resolved.
  • No in-cell measurement of acylated/unacylated ACP partitioning under metabolic perturbation
  • Mechanism allocating ACP between mitoribosome and ETC/Fe-S pools unknown
  • No human disease-causing mutation defined despite mechanistic centrality

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 2 GO:0140104 molecular carrier activity 2 GO:0098772 molecular function regulator activity 1
Localization
GO:0005739 mitochondrion 3
Pathway
R-HSA-1430728 Metabolism 2 R-HSA-8953854 Metabolism of RNA 1
Partners
FXNISCUISD11NDUFA6NDUFB9NFS1
Complex memberships
NFS1-ISD11-ACP (SDA) cysteine desulfurase complexRespiratory Complex Imitoribosome

Evidence

Reading pass · 8 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2019 NDUFAB1 (mitochondrial acyl carrier protein) functions not only as a Complex I subunit but also coordinates the assembly of respiratory complexes I, II, and III, and supercomplexes, by regulating iron-sulfur (Fe-S) cluster biosynthesis and stabilizing Complex I subunits. Cardiac-specific deletion caused defective bioenergetics, elevated ROS, and progressive dilated cardiomyopathy; overexpression enhanced mitochondrial bioenergetics and reduced ROS in ischemia-reperfusion injury. Cardiac-specific Ndufab1 knockout mice, NDUFAB1 overexpression in mice, mitochondrial bioenergetics assays, ROS measurement, respiratory complex and supercomplex assembly analysis Cell research High 31366990
2019 NDUFAB1 coordinately enhances lipoylation and activation of pyruvate dehydrogenase via the mitochondrial fatty acid synthesis (mtFAS) pathway, and increases respiratory complex and supercomplex assembly. Skeletal muscle-specific ablation caused systemic disruption of glucose homeostasis, defective insulin signaling, growth arrest, and early death. Overexpression protected mice against high-fat diet-induced obesity and insulin resistance. Skeletal muscle-specific Ndufab1 knockout mice, NDUFAB1 overexpression in mice, pyruvate dehydrogenase lipoylation assays, glucose homeostasis and insulin signaling measurements, mitochondrial complex assembly analysis FASEB journal High 31530015
2017 The acylated form of mitochondrial ACP (NDUFAB1) occupies the hydrophobic core of ISD11 via its 4'-phosphopantetheine-conjugated acyl chain, explaining the structural basis of ACP stabilization of ISD11 within the NFS1-ISD11-ACP (SDA) cysteine desulfurase complex that forms the core of the Fe-S assembly machinery. The structure revealed an unexpected dimeric ISD11 core architecture distinct from prokaryotic assemblies. X-ray crystallography of SDA complex, electron microscopy, enzyme kinetics, cell-based functional studies Proceedings of the National Academy of Sciences of the United States of America High 28634302
2018 The acylated form of ACP (NDUFAB1) acts as an acetyl-CoA-dependent allosteric activator of the LYR protein family (LYRM proteins) to stimulate electron transport chain (ETC) biogenesis. Acylation state of ACP, which depends on acetyl-CoA availability, coordinates ETC complex assembly with substrate availability. Biochemical assays demonstrating ACP acylation dependence on acetyl-CoA, interaction studies between acylated ACP and LYR proteins, genetic and functional studies of ETC assembly Molecular cell High 30118679
2017 Mitochondrial ACP (ACPM/NDUFAB1) binds to Complex I via LYR-motif containing subunits LYRM3 (NDUFB9) and LYRM6 (NDUFA6); two ACPM paralogs in Yarrowia lipolytica form ACPM1-LYRM6 and ACPM2-LYRM3 modules essential for complex I activity/assembly. The presence of a long acyl chain on the phosphopantetheine cofactor is required for docking ACPMs to protein complexes. ACPM1 is also found in the soluble LYRM4(ISD11)/NFS1 complex involved in Fe-S cluster biogenesis. Native gel electrophoresis, mass spectrometry, biochemical fractionation, functional analysis of complex I activity, acylation state analysis Biochimica et biophysica acta. Molecular cell research High 28802701
2019 The crystal structure of the human mitochondrial ACP-ISD11 heterodimer was determined at 2.0 Å resolution, showing that ACP and ISD11 form a cooperative unit stabilized by ionic interactions, hydrogen bonds, and apolar interactions. The 4'-phosphopantetheine-acyl chain covalently bound to ACP interacts with ISD11 residues, modulating ISD11 foldability. Recombinant human ACP-ISD11 interacts with NFS1 desulfurase to yield an active enzyme, and the core complex is activated by frataxin and ISCU. X-ray crystallography (2.0 Å), molecular dynamics simulations, in vitro NFS1 activity assays with frataxin and ISCU Biochemistry High 31664822
2019 Mitochondrial ACP (NDUFAB1) holo-protein participates as a component of mammalian mitoribosomes in its unacylated form, while acylated ACP species interact as partners in ACP-LYRM protein heterodimers that serve as assembly factors or subunits of the electron transport chain and Fe-S cluster assembly complexes. Octanoyl-ACP provides the C8 backbone for endogenous lipoic acid synthesis. Acyl-ACPs are proposed to act as signaling molecules coordinating mitochondrial acetyl-CoA levels with respiration, Fe-S cluster biogenesis, and protein lipoylation. Review integrating biochemical fractionation, interaction studies, and metabolic labeling data from multiple studies Biochimica et biophysica acta. Molecular cell research Medium 31473256
1999 The human NDUFAB1 protein contains a phosphopantetheine attachment site (DLGLDSLDQVEIIMAM) characteristic of acyl carrier proteins, an EF-hand calcium binding domain, and is a nuclear-encoded subunit of mitochondrial respiratory Complex I. No mutations in NDUFAB1 open reading frame were found in 20 patients with isolated Complex I deficiency. cDNA cloning and sequencing, sequence analysis, Northern blotting, mutational analysis by cDNA sequencing of patient fibroblasts Journal of inherited metabolic disease Medium 10234612

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2015 Acalabrutinib (ACP-196) in Relapsed Chronic Lymphocytic Leukemia. The New England journal of medicine 749 26641137
2017 Acalabrutinib (ACP-196): A Covalent Bruton Tyrosine Kinase Inhibitor with a Differentiated Selectivity and In Vivo Potency Profile. The Journal of pharmacology and experimental therapeutics 300 28882879
1995 Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases. The Plant cell 204 7734968
2003 SDAP: database and computational tools for allergenic proteins. Nucleic acids research 196 12520022
2016 Acalabrutinib (ACP-196): a selective second-generation BTK inhibitor. Journal of hematology & oncology 194 26957112
2002 Characterization of substrate specificity of plant FatA and FatB acyl-ACP thioesterases. Archives of biochemistry and biophysics 191 12061798
2000 Identification and analysis of the acyl carrier protein (ACP) docking site on beta-ketoacyl-ACP synthase III. The Journal of biological chemistry 154 11078736
2008 The IL-1 receptor accessory protein (AcP) is required for IL-33 signaling and soluble AcP enhances the ability of soluble ST2 to inhibit IL-33. Cytokine 149 18450470
2017 Structure of human Fe-S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP-ISD11 interactions. Proceedings of the National Academy of Sciences of the United States of America 127 28634302
2011 Phylogenetic and experimental characterization of an acyl-ACP thioesterase family reveals significant diversity in enzymatic specificity and activity. BMC biochemistry 124 21831316
2001 Putative ACP phosphodiesterase gene (acpD) encodes an azoreductase. The Journal of biological chemistry 121 11583992
2003 Catalysis, specificity, and ACP docking site of Streptomyces coelicolor malonyl-CoA:ACP transacylase. Structure (London, England : 1993) 114 12575934
2006 Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. Proceedings of the National Academy of Sciences of the United States of America 104 16983083
2014 Evolution of the AKH/corazonin/ACP/GnRH receptor superfamily and their ligands in the Protostomia. General and comparative endocrinology 100 25058364
2024 The modified RNA base acp3U is an attachment site for N-glycans in glycoRNA. Cell 91 39173631
2019 NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. Cell research 89 31366990
2020 Loss of m1acp3Ψ Ribosomal RNA Modification Is a Major Feature of Cancer. Cell reports 87 32375039
2014 The chain-flipping mechanism of ACP (acyl carrier protein)-dependent enzymes appears universal. The Biochemical journal 85 24825445
2001 Tartrate-resistant acid phosphatase (Acp 5): identification in diverse human tissues and dendritic cells. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 85 11373314
2018 ACP Acylation Is an Acetyl-CoA-Dependent Modification Required for Electron Transport Chain Assembly. Molecular cell 80 30118679
2012 Structure of the enzyme-acyl carrier protein (ACP) substrate gatekeeper complex required for biotin synthesis. Proceedings of the National Academy of Sciences of the United States of America 79 23045647
2012 Targeting InhA, the FASII enoyl-ACP reductase: SAR studies on novel inhibitor scaffolds. Current topics in medicinal chemistry 75 22283812
2013 Metabolic engineering of fatty acyl-ACP reductase-dependent pathway to improve fatty alcohol production in Escherichia coli. Metabolic engineering 70 24333607
2007 Mechanism and substrate recognition of human holo ACP synthase. Chemistry & biology 70 18022563
2021 Machine learning-guided acyl-ACP reductase engineering for improved in vivo fatty alcohol production. Nature communications 68 34611172
2006 Kinetic, inhibition and structural studies on 3-oxoacyl-ACP reductase from Plasmodium falciparum, a key enzyme in fatty acid biosynthesis. The Biochemical journal 63 16225460
1999 Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (FabD). Journal of bacteriology 63 10464226
2020 Gating mechanism of elongating β-ketoacyl-ACP synthases. Nature communications 60 32265440
2000 ACP Best Practice No 163. Wilson's disease: acute and presymptomatic laboratory diagnosis and monitoring. Journal of clinical pathology 51 11127261
2019 Mitochondrial acyl carrier protein (ACP) at the interface of metabolic state sensing and mitochondrial function. Biochimica et biophysica acta. Molecular cell research 49 31473256
2013 The adhesin complex protein (ACP) of Neisseria meningitidis is a new adhesin with vaccine potential. mBio 46 23443003
2007 PET analysis of the 5-HT2A receptor inverse agonist ACP-103 in human brain. The international journal of neuropsychopharmacology 38 17708779
2003 Detecting potential IgE-reactive sites on food proteins using a sequence and structure database, SDAP-food. Journal of agricultural and food chemistry 37 14705920
2002 Kinetic and mechanistic analysis of the malonyl CoA:ACP transacylase from Streptomyces coelicolor indicates a single catalytically competent serine nucleophile at the active site. Biochemistry 37 11814333
2000 Widespread expression of tartrate-resistant acid phosphatase (Acp 5) in the mouse embryo. Journal of anatomy 37 10853965
2017 Acyl modification and binding of mitochondrial ACP to multiprotein complexes. Biochimica et biophysica acta. Molecular cell research 36 28802701
2002 Enzymatic assembly of epothilones: the EpoC subunit and reconstitution of the EpoA-ACP/B/C polyketide and nonribosomal peptide interfaces. Biochemistry 36 11969430
2011 Substrate recognition by β-ketoacyl-ACP synthases. Biochemistry 35 22017312
2009 Structure and malonyl CoA-ACP transacylase binding of streptomyces coelicolor fatty acid synthase acyl carrier protein. ACS chemical biology 35 19555075
1999 The human nuclear-encoded acyl carrier subunit (NDUFAB1) of the mitochondrial complex I in human pathology. Journal of inherited metabolic disease 34 10234612
1999 Polyketide synthase acyl carrier protein (ACP) as a substrate and a catalyst for malonyl ACP biosynthesis. Chemistry & biology 34 10421763
2021 Neuropeptide ACP facilitates lipid oxidation and utilization during long-term flight in locusts. eLife 32 34151772
2016 A new strategy for strain improvement of Aurantiochytrium sp. based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase. Enzyme and microbial technology 31 27702480
2007 Pharmacokinetics, tolerability, and safety of ACP-103 following single or multiple oral dose administration in healthy volunteers. Journal of clinical pharmacology 31 17473118
2017 Combined BTK and PI3Kδ Inhibition with Acalabrutinib and ACP-319 Improves Survival and Tumor Control in CLL Mouse Model. Clinical cancer research : an official journal of the American Association for Cancer Research 30 28645939
2015 Expression of Cyanobacterial Acyl-ACP Reductase Elevates the Triacylglycerol Level in the Red Alga Cyanidioschyzon merolae. Plant & cell physiology 30 26272551
2010 Characterization of Acp, a peptidoglycan hydrolase of Clostridium perfringens with N-acetylglucosaminidase activity that is implicated in cell separation and stress-induced autolysis. Journal of bacteriology 30 20190047
2006 ACP-103, a 5-HT2A receptor inverse agonist. Current opinion in investigational drugs (London, England : 2000) 30 16869120
2019 NDUFAB1 protects against obesity and insulin resistance by enhancing mitochondrial metabolism. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29 31530015
2018 Mutation in the putative ketoacyl-ACP reductase CaKR1 induces loss of pungency in Capsicum. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 29 30267113
2006 Cloning and functional expression of an acyl-ACP thioesterase FatB type from Diploknema (Madhuca) butyracea seeds in Escherichia coli. Plant physiology and biochemistry : PPB 29 17092734
1994 Expression of mRNA and steady-state levels of protein isoforms of enoyl-ACP reductase from Brassica napus. Plant molecular biology 29 7948866
2018 The Fatty Acid Synthesis Protein Enoyl-ACP Reductase II (FabK) is a Target for Narrow-Spectrum Antibacterials for Clostridium difficile Infection. ACS infectious diseases 28 30501172
2017 Structure of the Neisseria Adhesin Complex Protein (ACP) and its role as a novel lysozyme inhibitor. PLoS pathogens 28 28662181
2010 A novel role of malonyl-ACP in lipid homeostasis. Biochemistry 28 20201588
2010 Mechanism and inhibition of the FabV enoyl-ACP reductase from Burkholderia mallei. Biochemistry 27 20055482
2016 Anatomy of the β-branching enzyme of polyketide biosynthesis and its interaction with an acyl-ACP substrate. Proceedings of the National Academy of Sciences of the United States of America 26 27573844
2010 Recognition of (2S)-aminomalonyl-acyl carrier protein (ACP) and (2R)-hydroxymalonyl-ACP by acyltransferases in zwittermicin A biosynthesis. Biochemistry 26 20353188
2020 α-proteobacteria synthesize biotin precursor pimeloyl-ACP using BioZ 3-ketoacyl-ACP synthase and lysine catabolism. Nature communications 25 33154364
2019 Structural insights into repression of the Pneumococcal fatty acid synthesis pathway by repressor FabT and co-repressor acyl-ACP. FEBS letters 25 31291684
2011 Characterization of molecular interactions between ACP and halogenase domains in the Curacin A polyketide synthase. ACS chemical biology 25 22103656
2005 ACP Best Practice No 181: Chemical pathology clinical investigation and management of nephrolithiasis. Journal of clinical pathology 25 15677531
2018 Structure of the Recombinant Neisseria gonorrhoeae Adhesin Complex Protein (rNg-ACP) and Generation of Murine Antibodies with Bactericidal Activity against Gonococci. mSphere 24 30305317
2013 Design, synthesis, and biological and crystallographic evaluation of novel inhibitors of Plasmodium falciparum enoyl-ACP-reductase (PfFabI). Journal of medicinal chemistry 24 24063369
1983 Purification and characterization of beta-ketoacyl-ACP synthetase I from Spinacia oleracea leaves. Archives of biochemistry and biophysics 23 6830245
2022 Identification and Characterization of ACP-5862, the Major Circulating Active Metabolite of Acalabrutinib: Both Are Potent and Selective Covalent Bruton Tyrosine Kinase Inhibitors . The Journal of pharmacology and experimental therapeutics 22 36310034
2019 Plastidial and Mitochondrial Malonyl CoA-ACP Malonyltransferase is Essential for Cell Division and Its Overexpression Increases Storage Oil Content. Plant & cell physiology 21 30796840
2011 Single particle tracking of acyl carrier protein (ACP)-tagged TrkA receptors in PC12nnr5 cells. Journal of neuroscience methods 21 22061422
2024 High expression of oleoyl-ACP hydrolase underpins life-threatening respiratory viral diseases. Cell 20 39137778
2023 ACP-Mediated Phase Transformation for Collagen Mineralization: A New Understanding of the Mechanism. Advanced healthcare materials 20 37742096
2021 Insights into Acinetobacter baumannii fatty acid synthesis 3-oxoacyl-ACP reductases. Scientific reports 20 33782435
2018 Cloning of the pks3 gene of Aurantiochytrium limacinum and functional study of the 3-ketoacyl-ACP reductase and dehydratase enzyme domains. PloS one 20 30533058
2014 Evolution of acyl-ACP-thioesterases and β-ketoacyl-ACP-synthases revealed by protein-protein interactions. Journal of applied phycology 20 25110394
2012 Advances in the research of β-ketoacyl-ACP synthase III (FabH) inhibitors. Current medicinal chemistry 20 22257054
2017 Discovery and Characterization of the 3-Hydroxyacyl-ACP Dehydratase Component of the Plant Mitochondrial Fatty Acid Synthase System. Plant physiology 19 28202596
2014 Identification and expression of a stearoyl-ACP desaturase gene responsible for oleic acid accumulation in Xanthoceras sorbifolia seeds. Plant physiology and biochemistry : PPB 19 25528221
2011 The β-ketoacyl-ACP synthase from Mycobacterium tuberculosis as potential drug targets. Current medicinal chemistry 19 21370994
2006 Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein (ACP) reductase: kinetic and chemical mechanisms. Biochemistry 19 17059223
1994 Acetoacyl-acyl carrier protein synthase from avocado: its purification, characterisation and clear resolution from acetyl CoA:ACP transacylase. Plant molecular biology 19 8018868
2014 Acyl-ACP substrate recognition in Burkholderia mallei BmaI1 acyl-homoserine lactone synthase. Biochemistry 18 25215658
2024 ACP-ML: A sequence-based method for anticancer peptide prediction. Computers in biology and medicine 17 38301519
2023 The Molecular Basis of Catalysis by SDR Family Members Ketoacyl-ACP Reductase FabG and Enoyl-ACP Reductase FabI in Type-II Fatty Acid Biosynthesis. Angewandte Chemie (International ed. in English) 17 37779101
2022 Computationally Guided Enzymatic Studies on Schizochytrium-Sourced Malonyl-CoA:ACP Transacylase. Journal of agricultural and food chemistry 17 36264009
2019 Structure of the Human ACP-ISD11 Heterodimer. Biochemistry 17 31664822
2024 ACP-CapsPred: an explainable computational framework for identification and functional prediction of anticancer peptides based on capsule network. Briefings in bioinformatics 16 39293807
2022 Metabolic engineering strategies to produce medium-chain oleochemicals via acyl-ACP:CoA transacylase activity. Nature communications 16 35338129
2012 Modeling holo-ACP:DH and holo-ACP:KR complexes of modular polyketide synthases: a docking and molecular dynamics study. BMC structural biology 16 22639887
2023 The updated Structural Database of Allergenic Proteins (SDAP 2.0) provides 3D models for allergens and incorporated bioinformatics tools. The journal of allergy and clinical immunology. Global 15 37781674
2019 Study on the atmospheric cold plasma (ACP) treatment of zein film: Surface properties and cytocompatibility. International journal of biological macromolecules 15 31756479
2016 A Review of Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP) and Enamel Remineralization. Compendium of continuing education in dentistry (Jamesburg, N.J. : 1995) 15 26863219
2005 Temporal and transient expression of olive enoyl-ACP reductase gene during flower and fruit development. Plant physiology and biochemistry : PPB 15 15763664
2002 Yellow lupine gene encoding stearoyl-ACP desaturase--organization, expression and potential application. Acta biochimica Polonica 15 12136953
2019 The prebiotic effect of CPP-ACP sugar-free chewing gum. Journal of dentistry 14 31693918
2010 Selectivity in a barren landscape: the P450(BioI)-ACP complex. Biochemical Society transactions 14 20658980
2021 A cryptic long-chain 3-ketoacyl-ACP synthase in the Pseudomonas putida F1 unsaturated fatty acid synthesis pathway. The Journal of biological chemistry 13 34181948
2021 Exposure-response analysis of acalabrutinib and its active metabolite, ACP-5862, in patients with B-cell malignancies. British journal of clinical pharmacology 13 34532877
2019 Single and combined BTK and PI3Kδ inhibition with acalabrutinib and ACP-319 in pre-clinical models of aggressive lymphomas. British journal of haematology 13 31355927
2018 Structural characterization of Porphyromonas gingivalis enoyl-ACP reductase II (FabK). Acta crystallographica. Section F, Structural biology communications 13 29400320
2016 Recent Advances and Structural Features of Enoyl-ACP Reductase Inhibitors of Mycobacterium tuberculosis. Archiv der Pharmazie 13 27775177
2006 Helicobacter pylori acyl carrier protein: expression, purification, and its interaction with beta-hydroxyacyl-ACP dehydratase. Protein expression and purification 13 17049879

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