Affinage

NDRG4

Protein NDRG4 · UniProt Q9ULP0

Length
352 aa
Mass
38.5 kDa
Annotated
2026-06-10
46 papers in source corpus 23 papers cited in narrative 23 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NDRG4 is a neuron- and cardiomyocyte-enriched cytoplasmic protein that couples intracellular signaling and vesicle trafficking to control cell proliferation, differentiation, migration, and survival across neural, cardiac, and epithelial tissues (PMID:18407257, PMID:21636852, PMID:27902705). In neurons it functions cell-autonomously to support sodium channel clustering at nodes of Ranvier and to maintain expression of the exocytic machinery snap25 and nsf, and is required for normal NGF-induced neurite outgrowth and downstream AP-1 and MEK/ERK signaling, while uncoupling ERK from nuclear Elk-1 activation in a microtubule-dependent manner (PMID:27902705, PMID:11978393, PMID:16408304). NDRG4-deficient mice show selectively reduced brain BDNF, impaired spatial learning, and enlarged ischemic infarcts, establishing a neuroprotective role (PMID:21636852). During cardiac development NDRG4 acts downstream of tbx5 to drive cardiomyocyte proliferation and proper heart morphogenesis (PMID:18407257). NDRG4 executes its cellular roles through defined physical partners: it binds Bves to direct fibronectin recycling-endosome fusion required for directional epithelial migration (PMID:24048452), binds CTMP to relieve CTMP inhibition of Akt and thereby activate CREB-driven myogenic differentiation (PMID:29254199), and binds p53 to restrain p53-mediated mitochondrial apoptosis (PMID:26053091, PMID:30593880). NDRG4 is epigenetically silenced by promoter hypermethylation in colorectal, breast, and other cancers, where it normally suppresses proliferation, invasion, and metastasis—acting through cell-cycle control (cyclin D1/p27, CDK4/6), integrin clustering and adhesion, and, in the enteric nervous system, by limiting paracrine secretion of Nid1/Fbln2 that drives colorectal carcinogenesis (PMID:19535783, PMID:30963110, PMID:32927604, PMID:33890711). A pathogenic missense variant (p.T256M) impairing cardiomyocyte proliferation links NDRG4 to pulmonary atresia with ventricular septal defect and tetralogy of Fallot (PMID:33211401).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 2001 Medium

    Establishing where NDRG4 is expressed was the prerequisite for any functional model; isoform mapping showed brain- and heart-restricted expression localized to neurons.

    Evidence Northern/Western blot, in situ hybridization and cDNA cloning of three splice isoforms

    PMID:11352569

    Open questions at the time
    • Isoform-specific functions not resolved
    • No subcellular localization defined at this stage
  2. 2002 Medium

    Subcellular fractionation answered where NDRG4 acts within the cell, placing it at mitochondria and as a membrane-associated, luminally oriented ER protein.

    Evidence Subcellular fractionation with detergent solubility and protease susceptibility assays in rat brain

    PMID:11978392

    Open questions at the time
    • Membrane topology determinants unknown
    • Functional consequence of ER/mitochondrial localization not tested here
  3. 2002 Medium

    Loss- and gain-of-function in PC12 cells linked NDRG4 levels to NGF-driven neurite outgrowth and AP-1 activation, giving it a role in neuronal differentiation signaling.

    Evidence Antisense and overexpression PC12 clones, neurite measurement, AP-1 reporter assay

    PMID:11978393

    Open questions at the time
    • Molecular mechanism connecting NDRG4 to AP-1 not defined
    • No direct binding partner identified
  4. 2006 Medium

    Pharmacological dissection refined the signaling role, showing NDRG4 enhances NGF-induced MEK/ERK phosphorylation yet attenuates nuclear Elk-1 activation via microtubules.

    Evidence Stable overexpression in PC12 cells, phospho-Western, Elk-1/SRE reporter, colchicine vs cytochalasin D

    PMID:16408304

    Open questions at the time
    • Mechanism of microtubule involvement unresolved
    • No direct ERK-pathway interactor shown
  5. 2008 High

    Zebrafish knockdown placed NDRG4 in cardiac development downstream of tbx5, answering whether its heart expression is functionally required for cardiomyocyte proliferation.

    Evidence Morpholino knockdown, in situ hybridization, tbx5/tbx20 genetic epistasis, histology

    PMID:18407257

    Open questions at the time
    • Direct transcriptional link from tbx5 to ndrg4 not shown
    • Effector mediating proliferation downstream of ndrg4 unknown
  6. 2009 High

    Parallel cancer studies established opposing context-dependent roles: NDRG4 is required for GBM survival but is a methylation-silenced tumor suppressor in colorectal cancer.

    Evidence siRNA knockdown with cell-cycle/apoptosis readouts and xenografts (GBM); overexpression assays plus methylation-specific PCR in CRC cohorts

    PMID:19535783 PMID:19592488

    Open questions at the time
    • Basis of tissue-specific oncogenic vs suppressive role unexplained
    • Direct targets of cell-cycle regulation not identified
  7. 2011 High

    The knockout mouse answered NDRG4's organismal neural function, showing it maintains brain BDNF, supports learning and memory, and protects against ischemic injury.

    Evidence Ndrg4 knockout mice, immunochemical neurotrophin profiling, Morris water maze, MCAO ischemia model

    PMID:21636852

    Open questions at the time
    • Mechanism by which NDRG4 maintains BDNF unresolved
    • Cell type responsible for protection not pinpointed
  8. 2013 High

    Identification of the Bves interaction provided the first direct molecular mechanism, linking NDRG4 to fibronectin recycling-endosome fusion and directional migration.

    Evidence Protein interaction mapping, fibronectin recycling assay, TIRF microscopy, directional migration assay

    PMID:24048452

    Open questions at the time
    • Structural basis of Bves/NDRG4 interaction unknown
    • Generality across cell types untested
  9. 2015 Medium

    Co-IP demonstrated NDRG4 binds p53 and restrains p53-mediated mitochondrial apoptosis, explaining the pro-survival side of its cancer phenotype.

    Evidence siRNA knockdown, Annexin-V/JC-1 assays, immunoprecipitation, PFT-α inhibition in meningioma cells

    PMID:26053091

    Open questions at the time
    • Direct binding interface not mapped
    • Single cell-type context
  10. 2016 High

    Cell-autonomous zebrafish analysis defined a neuronal mechanism: NDRG4 controls node-of-Ranvier sodium channel clustering and the snap25/nsf exocytic machinery.

    Evidence ndrg4 mutant and chimeric larvae, in situ hybridization, immunofluorescence, snap25/nsf expression analysis

    PMID:27902705

    Open questions at the time
    • How NDRG4 regulates snap25/nsf transcription unknown
    • Direct involvement in vesicle fusion not biochemically shown
  11. 2016 High

    Rigorous cell-type mapping established that NDRG4 is exclusively neuronal, including the enteric nervous system, refining all downstream interpretation.

    Evidence Immunohistochemistry, in situ hybridization, confocal immunofluorescence with HuC/D and GFAP markers, KO mouse validation

    PMID:28524415

    Open questions at the time
    • Subtype specificity within neurons not resolved
  12. 2017 Medium

    The CTMP interaction explained how NDRG4 activates Akt/CREB signaling to promote myogenic differentiation.

    Evidence C2C12 gain/loss-of-function, co-IP of NDRG4-CTMP, phospho-Akt/CREB Western, myotube and qRT-PCR assays

    PMID:29254199

    Open questions at the time
    • Whether CTMP regulation operates in neural/cardiac contexts untested
    • Binding interface not defined
  13. 2020 Medium

    Cancer mechanism studies expanded NDRG4's suppressive repertoire to integrin reorganization, CDK/Cyclin control, and mitochondrial function across breast, esophageal, and pancreatic models.

    Evidence Knockdown/overexpression with integrin clustering and adhesion assays, CDK4/6/Cyclin D1 Western, mitochondrial ATP/membrane-potential/morphology assays

    PMID:30963110 PMID:32927604 PMID:33298240

    Open questions at the time
    • Direct molecular partners for integrin and mitochondrial effects not identified
    • Single-lab findings per tumor type
  14. 2020 Medium

    miRNA studies positioned NDRG4 as a regulated downstream effector in ischemia/reperfusion injury, with miR-218-5p and miR-433 directly targeting it.

    Evidence Luciferase reporter validation, miRNA mimic/inhibitor and siRNA rescue in OGD/R PC12 and H9c2 cells, in vivo IR models

    PMID:32048632 PMID:32187107

    Open questions at the time
    • Whether NDRG4 itself drives PI3K/Akt protection mechanistically unresolved
    • Physiological miRNA-NDRG4 axis in vivo not fully established
  15. 2021 High

    Combined in vivo and proteomic work showed enteric-neuronal NDRG4 loss drives colorectal carcinogenesis through paracrine secretion of Nid1/Fbln2, and a separate study linked NDRG4 to 5-FU chemosensitivity via DDIT3.

    Evidence Ndrg4 KO CRC mouse models, ENS-organoid co-culture, secretome proteomics; overexpression with DDIT3 knockdown rescue and apoptosis assays

    PMID:33890711 PMID:34594423

    Open questions at the time
    • How neuronal NDRG4 loss controls Nid1/Fbln2 secretion mechanistically unknown
    • Receptor on tumor cells for paracrine signal not identified
  16. 2021 Medium

    Functional characterization of a patient missense variant provided direct evidence linking NDRG4 to congenital heart disease.

    Evidence Exome sequencing and p.T256M functional assays in human cardiac myocytes (proliferation, cell cycle, p27/caspase-9 Western)

    PMID:33211401

    Open questions at the time
    • Single variant in limited patient set
    • Mechanism by which T256M impairs proliferation not resolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • The biochemical activity of NDRG4 itself—whether it has intrinsic catalytic, scaffolding, or adaptor function that unifies its diverse interactions with Bves, CTMP, and p53—remains undefined.
  • No defined enzymatic or biochemical activity
  • No structural model linking partner interactions
  • Mechanism unifying neuronal, cardiac, and tumor-suppressive roles unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 3
Localization
GO:0005739 mitochondrion 2 GO:0005783 endoplasmic reticulum 1
Pathway
R-HSA-1643685 Disease 3 R-HSA-162582 Signal Transduction 2 R-HSA-5357801 Programmed Cell Death 2 R-HSA-5653656 Vesicle-mediated transport 2 R-HSA-1266738 Developmental Biology 1
Partners
BVESCTMPTP53

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 NDRG4 is expressed as three mRNA isoforms (NDRG4-B, NDRG4-B(var), and NDRG4-H) generated by alternative splicing and possibly alternative promoter usage. NDRG4-B is expressed only in brain, while NDRG4-H is expressed in both brain and heart. In situ hybridization localized NDRG4 to neurons of the brain and spinal cord. Northern blot, Western blot, in situ hybridization, cDNA cloning Genomics Medium 11352569
2002 NDRG4 protein is distributed mainly in mitochondria and endoplasmic reticulum (ER) in cerebral subcellular fractions. In the ER, NDRG4 protein is membrane-associated and luminally oriented, as demonstrated by detergent solubility assays and protease susceptibility experiments. Four protein isoforms (38, 39, 41, and 45 kDa) are differentially expressed during rat brain development. Subcellular fractionation, detergent solubility assay, protease susceptibility assay, Western blot Brain research. Developmental brain research Medium 11978392
2002 Reduction of NDRG4 protein levels by antisense transfection in PC12 cells inhibits NGF-induced neurite outgrowth and suppresses NGF-mediated AP-1 transcription factor activation. Conversely, NDRG4-overexpressing clones show enhanced AP-1 activation, linking NDRG4 levels to AP-1 signaling downstream of NGF. Antisense stable transfection, neurite length measurement, reporter assay (AP-1 activation), NGF stimulation Brain research. Developmental brain research Medium 11978393
2006 PC12 cells stably overexpressing Ndrg4 show enhanced NGF-induced phosphorylation of MEK and ERK, but attenuated phosphorylation of the nuclear ERK target Elk-1. The suppression of Elk-1 activation by Ndrg4 is abolished by colchicine (microtubule depolymerization) but not cytochalasin D (actin depolymerization), implicating microtubules in Ndrg4-mediated uncoupling of ERK from Elk-1. Stable overexpression in PC12 cells, phospho-Western blot, reporter assay (Elk-1/SRE), pharmacological inhibition (colchicine, cytochalasin D) Journal of cellular biochemistry Medium 16408304
2008 Ndrg4 knockdown in zebrafish embryos causes reduced cardiomyocyte proliferation, hypoplastic hearts, abnormal heart looping, and altered expression of versican and bmp4 in the atrioventricular canal. Ndrg4 expression is positively regulated by tbx5 (loss of tbx5 decreases ndrg4; gain of tbx5 via tbx20 knockdown increases ndrg4), placing ndrg4 downstream of tbx5 in cardiac development. Morpholino knockdown in zebrafish, in situ hybridization, genetic epistasis (tbx5/tbx20 mutants), histology Developmental biology High 18407257
2009 NDRG4 knockdown in GBM cells causes G1 cell cycle arrest (associated with decreased cyclin D1 and increased p27Kip1) followed by apoptosis (associated with decreased XIAP and survivin), and reduces tumorigenic capacity in intracranial xenograft models. NDRG4 overexpression has no effect on cell viability. siRNA knockdown, flow cytometry (cell cycle), Western blot, intracranial xenograft assay The Journal of biological chemistry Medium 19592488
2009 NDRG4 overexpression in colorectal cancer cell lines suppresses colony formation, cell proliferation, and invasion, establishing a tumor suppressive function. NDRG4 promoter is methylated in ~86% of colorectal cancers compared with ~4% of noncancerous colon mucosa, and methylation correlates with reduced mRNA and protein expression. Stable transfection/overexpression, colony formation assay, cell proliferation assay, invasion assay, methylation-specific PCR, bisulfite sequencing, RT-PCR, immunohistochemistry Journal of the National Cancer Institute High 19535783
2011 NDRG4-deficient mice show decreased brain BDNF protein levels (with normal GDNF, NGF, NT-3, TGF-β1), impaired spatial learning and memory, and greater brain infarct sizes after focal ischemia, establishing that NDRG4 maintains intracerebral BDNF levels and confers resistance to ischemic neuronal death. Knockout mouse model, immunochemical analysis, Morris water maze, focal ischemia (MCAO) model The Journal of biological chemistry High 21636852
2012 NDRG4 knockdown in meningioma cell lines causes reduced cell survival, DNA fragmentation, and G2-M cell cycle arrest, as well as decreased cellular invasion and migration. Conditioned media from NDRG4-depleted meningioma cells abrogates capillary tube formation, proliferation, and invasion of brain endothelial cells, indicating a paracrine role in angiogenesis. siRNA knockdown, spheroid/wound healing/Boyden chamber invasion assays, 3D invasion assay, endothelial tube formation assay with conditioned media Integrative biology Medium 22869042
2013 NDRG4 physically interacts with Bves (blood vessel epicardial substance). This Bves/NDRG4 interaction is required for trafficking of internalized fibronectin through the autocrine ECM deposition recycling pathway and for fusion of recycling endosomes with the basal cell surface, which is necessary for directional epicardial cell movement. Protein-protein interaction (novel interaction), fibronectin recycling assay, TIRF microscopy, directional migration assay, functional disruption of Bves/NDRG4 interaction Molecular biology of the cell High 24048452
2015 NDRG4 silencing in meningioma cells induces apoptosis via p53-mediated mitochondrial pathway: p53 is upregulated and phosphorylated at Ser15, BAX is activated, Bcl-2/Bcl-xL are downregulated, cytochrome c is released, and caspases are executed. Immunoprecipitation confirmed direct binding of NDRG4 to p53. A p53 phosphorylation inhibitor (PFT-α) protects against NDRG4-depletion-induced apoptosis. siRNA knockdown, Annexin-V flow cytometry, Western blot, mitochondrial membrane potential assay (JC-1), immunoprecipitation, immunofluorescence, pharmacological inhibition (PFT-α) Oncotarget Medium 26053091
2016 In zebrafish, ndrg4 functions cell-autonomously within neurons (established by chimeric larvae analysis) to regulate sodium channel clustering at nodes of Ranvier. Loss of ndrg4 sharply decreases expression of snap25 and nsf, implicating ndrg4 in control of vesicle exocytosis via the t-SNARE/NSF machinery. Zebrafish ndrg4 mutants, chimeric larvae analysis, in situ hybridization, immunofluorescence, molecular analysis of snap25/nsf expression PLoS genetics High 27902705
2016 NDRG4 is expressed exclusively in neurons (not glia) throughout the body including the enteric nervous system, colocalizing with HuC/D (pan-neuronal marker) but not GFAP (enteric glial marker), as established by immunohistochemistry, Western blot, in situ mRNA hybridization, and confocal immunofluorescence on wild-type vs. knockout mouse tissues. Immunohistochemistry, Western blot, in situ mRNA hybridization, immunofluorescence/confocal microscopy, NDRG4 knockout mouse validation Neurogastroenterology and motility High 28524415
2017 NDRG4 promotes myogenic differentiation by binding to CTMP (carboxyl-terminal modulator protein), thereby reducing the CTMP-Akt interaction, increasing Akt phosphorylation, and activating CREB, which drives expression of MyoD, myogenin, and myosin heavy chain. Gain/loss-of-function in C2C12 cells, co-immunoprecipitation (NDRG4-CTMP interaction), Western blot (p-Akt, p-CREB), myotube formation assay, qRT-PCR Oncotarget Medium 29254199
2018 NDRG4 interacts with p53 (confirmed by co-IP and immunofluorescence) and inhibits p53 expression and p53-mediated mitochondrial apoptosis signaling in cerebral ischemia/reperfusion injury. NDRG4 overexpression decreases infarct size; conversely, p53 in turn inhibits NDRG4 expression after I/R injury, creating a regulatory feedback. MCAO rat model, adenoviral overexpression, co-immunoprecipitation, immunofluorescence, Western blot, infarct size measurement Brain research bulletin Medium 30593880
2019 Epigenetic silencing of NDRG4 via promoter hypermethylation in breast cancer cells promotes integrin signaling: NDRG4 downregulation assembles β1-integrins into large punctate clusters at the leading edge of tumor cells, causing decreased adhesion to fibronectin and increased adhesion and migration towards vitronectin, facilitating lymph node metastasis. Stable knockdown, integrin clustering assay (immunofluorescence), adhesion/migration assays to fibronectin and vitronectin, methylation analysis, clinical cohort correlation NPJ breast cancer Medium 30963110
2020 miR-218-5p directly targets NDRG4 (validated by luciferase reporter assay). In OGD/R-injured PC12 cells, miR-218-5p inhibition upregulates NDRG4 and reduces inflammatory cytokines, oxidative stress, and apoptosis; NDRG4 silencing abolishes these protective effects, placing NDRG4 as a functional downstream target of miR-218-5p. Luciferase reporter assay, miRNA inhibitor transfection, siRNA knockdown, flow cytometry, Western blot, OGD/R model Medical science monitor Medium 32048632
2020 miR-433 directly targets NDRG4 (validated by dual-luciferase reporter assay and Western blot). miR-433 inhibition activates the PI3K/Akt pathway and protects against hypoxia/reoxygenation injury in H9c2 cardiomyocytes; NDRG4 silencing reverses these protective effects, placing NDRG4 as an effector of miR-433 in the PI3K/Akt pathway during myocardial I/R injury. Dual-luciferase reporter assay, Western blot, miRNA mimic/inhibitor transfection, siRNA knockdown, flow cytometry, in vivo rat IR model with antagomiR Shock Medium 32187107
2021 Loss of Ndrg4 in enteric neurons is associated with enlarged intestinal adenoma development. Ndrg4-/- enteric nervous system cell secretome, enriched for Nidogen-1 (Nid1) and Fibulin-2 (Fbln2), boosts organoid growth and enhances CRC cell migration, linking NDRG4 in enteric neurons to paracrine regulation of colorectal carcinogenesis. Ndrg4 knockout mouse CRC models, indirect co-culture of ENS cells and intestinal organoids, quantitative proteomics of secretome, migration assay, in vivo adenoma models EMBO reports High 33890711
2021 NDRG4 sensitizes colorectal cancer cells to 5-FU by upregulating DDIT3 (CHOP) expression. NDRG4 inhibits PI3K/AKT and ERK signaling, promotes 5-FU-induced apoptosis, and this proapoptotic effect is dependent on DDIT3. Stable overexpression, cell proliferation assay, Western blot (PI3K/AKT, ERK), DDIT3 knockdown rescue experiment, apoptosis assay Oncology letters Medium 34594423
2021 A missense variant p.T256M in NDRG4 impairs proliferation of human cardiac myocytes (hCM) and causes G1 and G2 cell cycle arrest with increased p27 and caspase-9 expression, providing evidence that this variant is pathogenic in pulmonary atresia with ventricular septal defect and tetralogy of Fallot. Patient exome sequencing, functional assay in hCM (proliferation, cell cycle analysis, Western blot for p27 and caspase-9) FEBS open bio Medium 33211401
2020 NDRG4 overexpression in esophageal adenocarcinoma cells downregulates Cyclin D1, CDK4, and CDK6 protein levels and inhibits tumor cell growth in 2D and 3D organotypic culture models, implicating NDRG4 in cell cycle regulation through CDK/Cyclin pathways. Stable overexpression, Western blot (Cyclin D1, CDK4, CDK6), 2D/3D organotypic growth assay, EdU proliferation assay Cancers Medium 32927604
2020 NDRG4 overexpression in pancreatic ductal adenocarcinoma cells attenuates mitochondrial function: reduces ATP production, decreases mitochondrial membrane potential, and increases mitochondrial fragmentation. NDRG4 knockdown in normal pancreatic cells produces opposite effects, establishing a direct role for NDRG4 in mitochondrial regulation. Stable overexpression and knockdown, ATP production assay, mitochondrial membrane potential assay, mitochondrial morphology analysis BMB reports Medium 33298240

Source papers

Stage 0 corpus · 46 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Characterization of the human NDRG gene family: a newly identified member, NDRG4, is specifically expressed in brain and heart. Genomics 200 11352569
2009 N-Myc downstream-regulated gene 4 (NDRG4): a candidate tumor suppressor gene and potential biomarker for colorectal cancer. Journal of the National Cancer Institute 178 19535783
2008 Ndrg4 is required for normal myocyte proliferation during early cardiac development in zebrafish. Developmental biology 68 18407257
2011 NDRG4 protein-deficient mice exhibit spatial learning deficits and vulnerabilities to cerebral ischemia. The Journal of biological chemistry 67 21636852
2014 Quantitative detection of methylated NDRG4 gene as a candidate biomarker for diagnosis of colorectal cancer. Oncology letters 57 25663916
2014 DNA methylation analysis of SFRP2, GATA4/5, NDRG4 and VIM for the detection of colorectal cancer in fecal DNA. Oncology letters 53 25202404
2009 NDRG4 is required for cell cycle progression and survival in glioblastoma cells. The Journal of biological chemistry 50 19592488
2013 Bves and NDRG4 regulate directional epicardial cell migration through autocrine extracellular matrix deposition. Molecular biology of the cell 40 24048452
2002 Inhibition of neurite outgrowth by reduced level of NDRG4 protein in antisense transfected PC12 cells. Brain research. Developmental brain research 40 11978393
2020 TFPI2 and NDRG4 gene promoter methylation analysis in peripheral blood mononuclear cells are novel epigenetic noninvasive biomarkers for colorectal cancer diagnosis. The journal of gene medicine 34 32196834
2021 Loss of enteric neuronal Ndrg4 promotes colorectal cancer via increased release of Nid1 and Fbln2. EMBO reports 33 33890711
2017 Is methylation analysis of SFRP2, TFPI2, NDRG4, and BMP3 promoters suitable for colorectal cancer screening in the Korean population? Intestinal research 33 29142517
2015 NDRG4 is a novel oncogenic protein and p53 associated regulator of apoptosis in malignant meningioma cells. Oncotarget 27 26053091
2012 NDRG4 is downregulated in glioblastoma and inhibits cell proliferation. Omics : a journal of integrative biology 26 22489821
2012 NDRG4, the N-Myc downstream regulated gene, is important for cell survival, tumor invasion and angiogenesis in meningiomas. Integrative biology : quantitative biosciences from nano to macro 24 22869042
2013 NDRG3 and NDRG4, two novel tumor-related genes. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 23 23725756
2018 NDRG4 protects against cerebral ischemia injury by inhibiting p53-mediated apoptosis. Brain research bulletin 22 30593880
2020 Downregulation of MiR-218-5p Protects Against Oxygen-Glucose Deprivation/Reperfusion-Induced Injuries of PC12 Cells via Upregulating N-myc Downstream Regulated Gene 4 (NDRG4). Medical science monitor : international medical journal of experimental and clinical research 21 32048632
2016 Neuronal Ndrg4 Is Essential for Nodes of Ranvier Organization in Zebrafish. PLoS genetics 20 27902705
2016 NDRG2 and NDRG4 Expression Is Altered in Glioblastoma and Influences Survival in Patients with MGMT-methylated Tumors. Anticancer research 18 26976975
2006 Ndrg4 enhances NGF-induced ERK activation uncoupled with Elk-1 activation. Journal of cellular biochemistry 18 16408304
2002 Molecular characterization of NDRG4/Bdm1 protein isoforms that are differentially regulated during rat brain development. Brain research. Developmental brain research 18 11978392
2019 NDRG4 promoter hypermethylation is a mechanistic biomarker associated with metastatic progression in breast cancer patients. NPJ breast cancer 16 30963110
2017 NDRG4 promotes myogenesis via Akt/CREB activation. Oncotarget 16 29254199
2019 NDRG4 prevents cerebral ischemia/reperfusion injury by inhibiting neuronal apoptosis. Genes & diseases 15 31832525
2018 NDRG4 in gastric cancer determines tumor cell proliferation and clinical outcome. Molecular carcinogenesis 15 29500881
2020 MiR-433 Regulates Myocardial Ischemia Reperfusion Injury by Targeting NDRG4 Via the PI3K/Akt Pathway. Shock (Augusta, Ga.) 13 32187107
2017 NDRG4, an early detection marker for colorectal cancer, is specifically expressed in enteric neurons. Neurogastroenterology and motility 13 28524415
2014 Exome sequencing identifies a novel homozygous variant in NDRG4 in a family with infantile myofibromatosis. European journal of medical genetics 12 25241110
2022 miR-23a-3p promotes the development of colon cancer by inhibiting the expression of NDRG4. Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 10 36374403
2016 Uterine Expression of NDRG4 Is Induced by Estrogen and Up-Regulated during Embryo Implantation Process in Mice. PloS one 10 27175791
2020 N-MYC Downstream Regulated Gene 4 (NDRG4), a Frequent Downregulated Gene through DNA Hypermethylation, plays a Tumor Suppressive Role in Esophageal Adenocarcinoma. Cancers 9 32927604
2016 Postnatal lethality and abnormal development of foregut and spleen in Ndrg4 mutant mice. Biochemical and biophysical research communications 9 26801554
2020 Hypermethylation-mediated silencing of NDRG4 promotes pancreatic ductal adenocarcinoma by regulating mitochondrial function. BMB reports 8 33298240
2004 Genomic organization, expression, and comparative analysis of noncoding region of the rat Ndrg4 gene. Gene 8 14693380
2024 LC-MS based metabonomics study on protective mechanism of ESWW in cerebral ischemia via CYTC/Apaf-1/NDRG4 pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology 7 38657364
2024 Evaluating the clinical performance of SDC2/NDRG4 methylation for colorectal cancer detection. Epigenomics 5 38226561
2021 NDRG4 sensitizes CRC cells to 5-FU by upregulating DDIT3 expression. Oncology letters 5 34594423
2022 NDRG4 Alleviates Myocardial Infarction-Induced Apoptosis through the JAK2/STAT3 Pathway. Computational and mathematical methods in medicine 4 35126626
2021 A loss-of-function mutation p.T256M in NDRG4 is implicated in the pathogenesis of pulmonary atresia with ventricular septal defect (PA/VSD) and tetralogy of Fallot (TOF). FEBS open bio 4 33211401
2020 NDRG4 Alleviates Aβ1-40 Induction of SH-SY5Y Cell Injury via Activation of BDNF-Inducing Signalling Pathways. Neurochemical research 4 32166572
2021 Highly sensitive fecal DNA testing of NDRG4 12b methylation is a promising marker for detection of colorectal precancerosis. Journal of B.U.ON. : official journal of the Balkan Union of Oncology 2 34564976
2026 NDRG4 overexpression is associated with reduced apoptosis after intracerebral hemorrhage via the PI3K/Akt/GSK3β signaling pathway. Scientific reports 0 41484166
2026 Targeting VAMP5 suppresses PLK1-driven growth of gliomas with high NDRG4 expression. Journal of neuro-oncology 0 42257837
2023 Retracted: NDRG4 Alleviates Myocardial Infarction-Induced Apoptosis through the JAK2/STAT3 Pathway. Computational and mathematical methods in medicine 0 38094327
2022 Erratum: NDRG4 sensitizes CRC cells to 5-FU by upregulating DDIT3 expression. Oncology letters 0 35707763

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