Affinage

NDEL1

Nuclear distribution protein nudE-like 1 · UniProt Q9GZM8

Length
345 aa
Mass
38.4 kDa
Annotated
2026-06-10
100 papers in source corpus 55 papers cited in narrative 51 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NDEL1 is a coiled-coil scaffold protein that functions as a central regulator of cytoplasmic dynein, controlling dynein recruitment and activation to drive neuronal migration, mitotic processes, and intracellular transport (PMID:11163260, PMID:15473966, PMID:16107726). It directly binds LIS1, the dynein intermediate chain, and dynactin, recruiting LIS1 to dynein through the conserved intermediate-chain N-terminus; because LIS1 cannot simultaneously occupy NDEL1 and dynein, NDEL1 must hand LIS1 off in discrete steps and is itself a negative regulator that disfavors dynein-dynactin-adaptor assembly until released (PMID:14970193, PMID:18784752, PMID:37730751, PMID:37086789). In neurons, NDEL1 acts cell-autonomously with LIS1 to couple the nucleus and centrosome during nucleokinesis, and its disruption underlies cortical migration defects, including the disease-associated p.R105P variant that ablates LIS1 binding (PMID:15473966, PMID:21092859, PMID:38194050). NDEL1 activity is gated by a dense layer of post-translational modification: CDK5/CDK1, Aurora-A, and Erk1/2 phosphorylation, palmitoylation, and S-nitrosylation each remodel its affinity for dynein, LIS1, and partner proteins to switch transport on or off in distinct compartments (PMID:12556484, PMID:17060449, PMID:19668197, PMID:19927128, PMID:22114287, PMID:26166569, PMID:27371763). Beyond dynein, NDEL1 organizes the centrosome and mother centriole, suppresses ciliogenesis through the trichoplein-Aurora A pathway, supports kinetochore dynein function, and bridges DISC1 into a DISC1-NDEL1-LIS1 complex relevant to neurite extension (PMID:12812986, PMID:14962739, PMID:16291865, PMID:26880200, PMID:17600710). It additionally engages cytoskeletal and signaling systems—neurofilament and keratin intermediate filaments, the WAVE regulatory complex, Cdc42GAP, and intermediate-filament transport—and possesses an intrinsic Cys273-dependent endooligopeptidase activity required for neurite outgrowth (PMID:15208636, PMID:22453242, PMID:18331715, PMID:15728732, PMID:34319758).

Mechanistic history

Synthesis pass · year-by-year structured walk · 19 steps
  1. 2000 High

    Established NDEL1 as a LIS1 partner and CDK5 substrate at centrosomes and growth cones, linking it to dynein and neuronal morphology and seeding the entire dynein-regulation model.

    Evidence Co-IP, subcellular fractionation, in vitro kinase assay and immunofluorescence in neurons

    PMID:11163260

    Open questions at the time
    • Did not define how phosphorylation alters dynein binding
    • No structural basis for LIS1 interaction
  2. 2002 High

    Identified DISC1 as an NDEL1 binding partner whose disease-truncation abolishes binding, connecting NDEL1 to a psychiatric-disease pathway and neurite extension.

    Evidence Yeast two-hybrid, Co-IP and neurite outgrowth in PC12 cells

    PMID:12506198

    Open questions at the time
    • Binding interface not mapped
    • Functional consequence beyond neurite length unknown
  3. 2003 High

    Mapped the DISC1-NDEL1-LIS1 trimolecular architecture and showed NDEL1 phosphorylation by CDK1/Erk1/2 and 14-3-3epsilon binding control its localization and LIS1 association, establishing NDEL1 as a phospho-regulated bridge.

    Evidence Two-hybrid and deletion mapping, in vitro kinase assays with phospho-mutants, mouse Ywhae genetics

    PMID:12556484 PMID:12796778 PMID:12812986 PMID:14962739

    Open questions at the time
    • Stoichiometry of the trimolecular complex unresolved
    • How 14-3-3 binding mechanistically stabilizes phosphorylation unclear
  4. 2004 High

    Demonstrated NDEL1 acts upstream of dynein and in a common pathway with LIS1, directly binding dynein heavy chain to drive membrane/organelle transport and nucleus-centrosome coupling.

    Evidence In utero RNAi with LIS1 rescue epistasis, dynein/LIS1-binding mutants, lysosome motility time-lapse, neurofilament binding and polymerization assays

    PMID:14970193 PMID:15208636 PMID:15473966

    Open questions at the time
    • Whether NDEL1 activates or recruits dynein not yet distinguished
    • Direct vs indirect effects on motor processivity untested
  5. 2005 High

    Revealed NDEL1 as a multifunctional effector—targeting dynein to microtubule plus ends via LIS1, linking CDK5 phosphorylation to katanin p60 distribution, and possessing intrinsic Cys273 endooligopeptidase activity inhibited by DISC1.

    Evidence Yeast null genetics with live imaging, Co-IP and migration assays, active-site mutagenesis with enzymatic and competitive-inhibition assays, knockout mouse

    PMID:15728732 PMID:15965467 PMID:16107726 PMID:16203747

    Open questions at the time
    • Physiological substrates of the peptidase activity unidentified
    • Relationship between scaffolding and enzymatic functions unclear
  6. 2006 High

    Placed NDEL1 in mitotic centrosome control, showing Aurora-A phosphorylation at Ser251 and direct TACC3 interaction drive centrosome maturation and separation.

    Evidence In vitro kinase assay, phosphomimetic rescue of Aurora-A depletion, Co-IP and immunofluorescence

    PMID:17060449

    Open questions at the time
    • How Ser251 phosphorylation alters dynein/LIS1 binding not defined
    • TACC3 recruitment mechanism incomplete
  7. 2007 High

    Defined NDEL1 kinetochore function—recruited via CENP-F to stabilize dynein/dynactin and ensure chromosome alignment—and solved the coiled-coil homodimer structure rationalizing cooperative LIS1 binding.

    Evidence Co-IP, siRNA with live imaging of chromosome behavior, X-ray crystallography of residues 10-166

    PMID:17494871 PMID:17600710 PMID:17997972

    Open questions at the time
    • C-terminal cargo/dynein region not crystallized
    • Distinct roles of NDEL1 vs NDE1 at kinetochores not fully separated
  8. 2008 High

    Resolved how NDEL1 phosphorylation is reversed and broadened its reach, showing PP4c dephosphorylates CDK1 sites to limit katanin recruitment, NDEL1 functions in prophase nuclear-envelope invagination, and it controls Cdc42 signaling, PDE4/cAMP scaffolding, and vimentin transport.

    Evidence In vitro phosphatase assay with genetic/pharmacological epistasis, siRNA with live imaging, competitive Cdc42GAP binding and migration assays, BRET and TAP-MS

    PMID:18303022 PMID:18331715 PMID:18347064 PMID:18784752 PMID:18809722 PMID:18845247

    Open questions at the time
    • Integration of these parallel functions in a single cell unclear
    • How LIS1 blocking of dynein is relieved mechanistically not fully defined
  9. 2009 High

    Established palmitoylation at the Cys273 region as a switch reducing dynein binding, defined NDEL1's role in retrograde axonal transport and adhesion dynamics, and placed it in an aPKC-Aurora-A-TPX2 cascade driving neurite microtubule emanation.

    Evidence Metabolic palmitoylation labeling with transport assays, antibody-blocking in DRG axons, paxillin/FAK Co-IP and adhesion assays, in vitro kinase and live MT imaging

    PMID:17202468 PMID:19492042 PMID:19522757 PMID:19668197 PMID:19927128

    Open questions at the time
    • Palmitoylating enzyme(s) not identified
    • Crosstalk between palmitoylation and phosphorylation untested
  10. 2010 High

    Positioned NDE1/NDEL1 upstream of LIS1 for membrane dynein recruitment, identified an N-terminal dynein-IC binding domain and lamin-B-dependent spindle matrix role, and rescued PDE-driven defects, consolidating NDEL1 as the membrane dynein adaptor.

    Evidence Double siRNA epistasis with membrane fractionation, Xenopus extract spindle reconstitution with mutagenesis, neurite-outgrowth rescue with catalytic mutant

    PMID:19198602 PMID:20048338 PMID:20462516 PMID:21056974

    Open questions at the time
    • How NDEL1/NDE1 associate with membranes molecularly unclear
    • Endopeptidase substrate during neurite outgrowth unknown
  11. 2011 High

    Defined the phosphorylation-gated motility switch: CDK5-phosphorylated NDEL1 forms a high-affinity LIS1/NDEL1/dynein complex that blocks ATP-dependent dynein release, and uncovered Dynamin 2 GTPase regulation, centrosomal microtubule anchoring, and additional partner contexts.

    Evidence Phospho-mutants and RNAi with axonal transport assays, in vitro GTPase assay, mutant mapping of centrosome targeting

    PMID:16291865 PMID:21283621 PMID:22114287 PMID:26166569

    Open questions at the time
    • Reconciliation of inhibitory vs activating roles across cell types incomplete
    • Quantitative kinetics of LIS1 handoff not yet measured
  12. 2012 High

    Determined NDEL1's higher-order architecture (dimers/tetramers/folded-back polymers) and expanded its functions to protein quality control (misfolded Gβ to aggresome) and WAVE regulatory complex assembly for actin polymerization.

    Evidence Negative-stain EM and crosslinking-MS, Co-IP and aggresome/half-life assays, in vitro actin polymerization and cell-spreading assays

    PMID:22430153 PMID:22453242 PMID:22843697

    Open questions at the time
    • Functional significance of polymeric self-assembly in cells unclear
    • How NDEL1 selects misfolded clients undefined
  13. 2013 Medium

    Placed NDEL1-dynein downstream of LIS1 for mitotic spindle orientation and showed cooperation with NudCL in retrograde mitochondrial transport.

    Evidence Lis1-mutant MEFs with rescue and live imaging, double siRNA with mitochondrial transport imaging and Co-IP

    PMID:23551859 PMID:24030547

    Open questions at the time
    • Direct vs indirect role of NDEL1 in spindle orientation not isolated
    • Mechanism of NudCL cooperation unclear
  14. 2016 High

    Identified NDEL1's localization-based control of cargo polarity at the AIS via Ankyrin-G and its suppression of ciliogenesis through trichoplein-Aurora A, plus actin-bundling cooperation with Tara.

    Evidence Co-IP and live cargo tracking with fractionation, siRNA and hypomorphic mouse with co-localization, migration and synergy assays

    PMID:26844830 PMID:26880200 PMID:27546710

    Open questions at the time
    • How AnkG recruitment couples to local dynein activation incompletely defined
    • Trigger for serum-starvation-induced NDEL1 degradation unknown
  15. 2017 High

    Provided structural and functional resolution of the DISC1-NDEL1 interface, linking it specifically to NDEL1 kinetochore attachment and mitotic timing in human cells, organoids, and patient iPSCs, and showed nNOS S-nitrosylation of Cys203 promotes dendritic arborization.

    Evidence X-ray crystallography with multi-system functional validation, biotin-switch S-nitrosylation assay with Cys203 mutant and Nos1 deletion

    PMID:27371763 PMID:29103808

    Open questions at the time
    • Whether S-nitrosylation intersects with dynein regulation untested
    • Interplay of DISC1-controlled kinetochore role with neuronal functions unclear
  16. 2019 High

    Solved the AnkB-NDEL1 5-helix bundle structure (2:1 stoichiometry) and demonstrated that disrupting the AnkG/NDEL1 complex disrupts AIS cargo sorting, cementing the structural basis of NDEL1 AIS recruitment.

    Evidence X-ray crystallography, Co-IP, live cargo tracking and peptide competition

    PMID:31889000

    Open questions at the time
    • In vivo requirement of this interface not tested
    • Link between ankyrin binding and dynein activation incomplete
  17. 2021 Medium

    Extended NDEL1's intermediate-filament role to keratins and desmosomes in epidermis, showing direct binding via a conserved IF motif and LIS1-dependent desmosomal localization.

    Evidence Direct binding assay, conditional knockout mouse with desmosome morphology analysis

    PMID:34319758

    Open questions at the time
    • Single-lab finding without independent replication
    • Whether keratin/neurofilament binding share a common mechanism untested
  18. 2023 High

    Reconstituted the mechanism with purified components: NDEL1 binds the conserved dynein IC N-terminus to recruit LIS1, but because LIS1 cannot bind NDEL1 and dynein simultaneously it must be handed off in discrete steps, and NDEL1 inhibits dynein-dynactin-adaptor assembly—an effect strengthened by phosphomimetic C-terminal mutations.

    Evidence Purified-protein binding, single-molecule imaging, in vitro dynein-dynactin-adaptor reconstitution and mutagenesis across two studies

    PMID:37086789 PMID:37730751

    Open questions at the time
    • Trigger that releases NDEL1 to permit handoff in cells unknown
    • How phosphorylation timing is coordinated with cargo loading unresolved
  19. 2024 High

    Provided direct disease causality, showing the p.R105P variant disrupts the NDEL1-LIS1 interface, impairs migration and nucleokinesis, validating the LIS1-binding interface as critical for cortical development.

    Evidence In utero electroporation, scRNA-seq and spatial transcriptomics, biochemical binding disruption, patient variant analysis

    PMID:38194050

    Open questions at the time
    • Full clinical spectrum of NDEL1 variants not defined
    • Whether other NDEL1 interfaces contribute to disease untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the dense array of NDEL1 modifications and binding partners is coordinated spatiotemporally to switch between dynein inhibition and activation in a given cell remains the central open question.
  • No unified model integrating phosphorylation, palmitoylation, and S-nitrosylation timing
  • In-cell trigger for LIS1 handoff from NDEL1 to dynein unidentified
  • Physiological substrates of the endooligopeptidase activity unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 5 GO:0008092 cytoskeletal protein binding 4 GO:0098772 molecular function regulator activity 4 GO:0016787 hydrolase activity 2 GO:0140096 catalytic activity, acting on a protein 2
Localization
GO:0005886 plasma membrane 4 GO:0005694 chromosome 3 GO:0005815 microtubule organizing center 3 GO:0005829 cytosol 3
Pathway
R-HSA-1266738 Developmental Biology 4 R-HSA-1640170 Cell Cycle 4 R-HSA-5653656 Vesicle-mediated transport 4 R-HSA-9609507 Protein localization 4 R-HSA-1852241 Organelle biogenesis and maintenance 2
Partners
14-3-3EPSILONANKYRIN-GCENP-FDISC1DYNEIN INTERMEDIATE CHAINKATANIN P60LIS1TACC3
Complex memberships
DISC1-NDEL1-LIS1 complexLIS1-NDEL1-dynein complexWAVE regulatory complex (WRC)dynein-dynactin-adaptor complex

Evidence

Reading pass · 51 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 NUDEL (NDEL1) was identified as a novel LIS1-interacting protein and a substrate of Cdk5 kinase. NUDEL is enriched at centrosomes and neuronal growth cones and interacts with cytoplasmic dynein. Inhibition of Cdk5 modifies NUDEL distribution in neurons and affects neuritic morphology. Co-immunoprecipitation, subcellular fractionation, in vitro kinase assay, immunofluorescence Neuron High 11163260
2003 14-3-3epsilon binds to CDK5/p35-phosphorylated NUDEL and this binding maintains NUDEL phosphorylation. Deficiency of 14-3-3epsilon results in mislocalization of NUDEL and LIS1, consistent with reduction of cytoplasmic dynein function. Co-immunoprecipitation, genetic mouse models (Ywhae knockout/heterozygous), immunolocalization Nature genetics High 12796778
2002 DISC1 interacts with NUDEL (NDEL1) via yeast two-hybrid, and the disease-associated truncation mutant of DISC1 fails to bind NUDEL. Expression of mutant DISC1 in PC12 cells reduces neurite extension. Yeast two-hybrid, co-immunoprecipitation, neurite outgrowth assay in PC12 cells Proceedings of the National Academy of Sciences of the United States of America High 12506198
2003 DISC1 interacts with NUDEL through a central coiled-coil domain of DISC1 that binds the C-terminal domain of NUDEL, independent from the LIS1 binding site on NUDEL. NUDEL acts as a bridge between DISC1 and LIS1, allowing formation of a trimolecular complex. Yeast two-hybrid, mammalian two-hybrid, co-immunoprecipitation, deletion mapping Human molecular genetics High 12812986 14962739
2004 Ndel1 positively regulates dynein activity by facilitating the interaction between LIS1 and dynein. Loss of Ndel1 in developing neocortex impairs neuronal positioning and causes uncoupling of the centrosome and nucleus. Overexpression of LIS1 partially rescues the positioning defect caused by Ndel1 RNAi, placing Ndel1 upstream of dynein and in common pathway with LIS1. RNA interference in developing neocortex (in utero), epistasis rescue experiments, immunofluorescence Neuron High 15473966
2003 Nudel (NDEL1) is specifically phosphorylated in M phase by Cdc2 (CDK1) and by Erk1/2. Phosphorylation regulates its cell-cycle-dependent distribution and increases its association with Lis1. A Nudel mutant incapable of binding Lis1 impaired poleward movement of dynein and dynein-mediated transport of kinetochore proteins to spindle poles. In vitro kinase assays, phosphorylation-mimicking and phosphorylation-deficient mutants, immunofluorescence, functional mitosis assays Molecular and cellular biology High 12556484
2005 Complete loss of Ndel1 results in embryonic lethality at the peri-implantation stage and cell proliferation defects. Compound heterozygous mice show neuronal migration defects. Ndel1 loss leads to abnormalities in microtubule organization and similar defects in dynein-dependent vesicle distribution as Lis1 loss. Rescue by LIS1, NDEL1, or NDE1 overexpression confirms they act in a common pathway. Targeted gene disruption (mouse knockout/hypomorph), MEF and granule cell assays, immunofluorescence, rescue experiments Molecular and cellular biology High 16107726
2005 CDK5-mediated phosphorylation of NDEL1 facilitates interaction between NDEL1 and katanin p60, and phosphorylated NDEL1 regulates the distribution of katanin p60. Loss of NDEL1 causes abnormal accumulation of p60 in nucleus. Loss of NDEL1 or dominant negative p60 in migrating neurons causes defective migration and elongation of nuclear-centrosomal distance. Co-immunoprecipitation, phosphorylation assays, dominant-negative expression, neuronal migration assay Human molecular genetics High 16203747
2006 Aurora-A phosphorylates NDEL1 at Ser251 at mitotic entry. This phosphorylation is required for centrosomal maturation and separation. NDEL1 is required for centrosome targeting of TACC3 through direct interaction with TACC3. Aurora-A phosphorylation-mimetic NDEL1 mutants rescue centrosomal maturation/separation defects in Aurora-A-depleted cells. In vitro kinase assay, phosphomimetic/phosphodeficient mutants, siRNA depletion rescue, immunofluorescence, co-immunoprecipitation Molecular and cellular biology High 17060449
2007 DISC1 directly interacts with kinesin-1 heavy chain and links the NUDEL/LIS1/14-3-3epsilon complex to kinesin-1 for anterograde axonal transport. Knockdown of DISC1 inhibited accumulation of NUDEL, LIS1, and 14-3-3epsilon at axons and inhibited axon elongation. Co-immunoprecipitation, RNAi knockdown, immunofluorescence, axon elongation assay The Journal of neuroscience Medium 17202468
2007 Cenp-F interacts with Ndel1 and Nde1, and Ndel1/Nde1/Lis1 localize to kinetochores in a Cenp-F-dependent manner. Inhibition of Ndel1 leads to chromosome malalignments not detected by the spindle checkpoint, resulting in lagging chromosomes during anaphase. Ndel1 and Nde1 play distinct roles at kinetochores. Co-immunoprecipitation, siRNA knockdown, live cell imaging, immunofluorescence Current biology High 17600710
2007 Ndel1 is required for robust localization of dynein/dynactin at the kinetochore. Kinetochore localization of Nudel depends mostly (~78%) on mitosin (CENP-F) and slightly on dynein/dynactin. Nudel activates dynein-mediated protein transport at the kinetochore and stabilizes kinetochore dynein/dynactin against microtubule-dependent stripping. RNAi, mutant overexpression, time-lapse imaging, immunofluorescence Molecular biology of the cell Medium 17494871
2007 Crystal structure of two fragments of the coiled-coil domain of Ndel1 (residues 10-166) revealed a stable parallel homodimer with supercoiled alpha helices. The structure suggests how the Lis1-interacting domain can be regulated via cooperative binding of two Ndel1 helices to a Lis1 homodimer. X-ray crystallography, solution studies (solution NMR/biophysics) Structure High 17997972
2004 NUDEL directly interacts with neurofilament light subunit (NF-L) and facilitates the polymerization of neurofilaments. Knockdown of NUDEL by RNAi destabilizes NF-L and alters neurofilament homeostasis, resulting in morphological changes reminiscent of neurodegeneration. Co-immunoprecipitation, RNAi knockdown in neuroblastoma cells and primary cortical neurons, in vitro polymerization assay Nature cell biology High 15208636
2004 Nudel (NDEL1) regulates dynein-mediated membrane transport through direct interactions with both Lis1 and dynein heavy chain. A Nudel mutant defective in Lis1 or dynein heavy chain binding causes dispersion of dynein-dependent membranous organelles and reduces frequency and velocity of lysosome minus-end-directed motions. Mutant overexpression, RNAi, time-lapse microscopy for lysosome motility, organelle localization assays The Journal of cell biology High 14970193
2005 Nudel (Ndl1 in budding yeast) targets dynein to microtubule plus ends through LIS1. The Ndl1 null mutant shows decreased targeting of dynein to microtubule plus ends. Ndl1 regulates dynein targeting through LIS1 but not through CLIP170. Yeast genetics (null mutant), live cell imaging, biochemical interaction assays Nature cell biology High 15965467
2005 Nudel (NDEL1) contributes to microtubule anchoring at the mother centriole. Nudel localizes to mother centriole and its centrosome localization requires a C-terminal region essential for associations with dynein, dynactin, PCM-1, pericentrin, and gamma-tubulin. Nudel plays roles in both dynein-mediated transport of dynactin/Lis1/PCM-1 and dynein-independent centrosomal targeting of pericentrin and gamma-tubulin. RNAi, mutant overexpression, immunofluorescence, co-immunoprecipitation Molecular biology of the cell Medium 16291865
2008 LIS1 suppresses the motility of cytoplasmic dynein on microtubules, while NDEL1 releases the blocking effect of LIS1 on cytoplasmic dynein. LIS1 mediates anterograde transport of cytoplasmic dynein as a dynein-LIS1 complex on transportable microtubule fragments. In vitro microtubule motility assay, blocking antibody experiments, co-immunoprecipitation The EMBO journal High 18784752
2008 Protein phosphatase 4 catalytic subunit (PP4c) dephosphorylates NDEL1 at CDK1 sites. Loss of PP4c leads to unscheduled CDK1 activation and abnormal NDEL1 phosphorylation, which causes excessive recruitment of katanin p60 to the centrosome and microtubule disorganization. Inhibition of CDK1, NDEL1, or katanin p60 rescues the defects caused by PP4 inhibition. In vitro phosphatase assay, targeted gene disruption, pharmacological CDK1 inhibition, epistasis rescue The Journal of cell biology High 18347064
2008 Lis1 and Ndel1 reduction impairs prophase nuclear envelope invagination (PNEI), a dynein-dependent process facilitating nuclear envelope breakdown (NEBD). Ndel1 phosphorylation is important for this function, regulating binding to both Lis1 and dynein. siRNA knockdown, live cell imaging, phosphomimetic/phosphodeficient mutants, mouse brain histology The Journal of cell biology Medium 18809722
2008 Nudel interacts with Cdc42GAP and competes with Cdc42 for binding Cdc42GAP, inhibiting Cdc42GAP-mediated inactivation of Cdc42 in a dose-dependent manner. Nudel's leading-edge localization in migrating cells requires phosphorylation by Erk1/2. Depleting Nudel or expressing a non-phosphorylatable mutant abolishes Cdc42 activation and cell migration. Co-immunoprecipitation, competitive binding assay, RNAi, phosphomimetic mutants, Cdc42 activation assay, migration assay Developmental cell High 18331715
2008 Ndel1 forms a novel complex with vimentin, dynein, Lis1, and alphaCOP. Ndel1 promotes interaction between Lis1, alphaCOP, and the vimentin-dynein complex, activating dynein-mediated transport of vimentin. Loss of Ndel1 by RNAi fails to incorporate Lis1/alphaCOP in the complex, reduces vimentin transport, and alters neuritogenesis. Tandem affinity purification, co-immunoprecipitation, RNAi, transport assay The Journal of biological chemistry Medium 18303022
2009 aPKC phosphorylates Aurora A at Thr287, which facilitates Aurora A interaction with TPX2 and activates Aurora A at the neurite hillock, leading to phosphorylation of NDEL1 at S251 and NDEL1 recruitment. Suppression of aPKC, Aurora A, TPX2, or Ndel1 impairs neurite extension. Suppression of this pathway decreases frequency of microtubule emanation from the MTOC. In vitro kinase assays, RNAi, phosphomimetic mutants, live microtubule plus-end marker imaging, immunofluorescence Nature cell biology High 19668197
2009 Palmitoylation of Ndel1 by specific palmitoylation enzymes occurs in vivo. Unpalmitoylated Ndel1 interacts better with dynein; palmitoylated Ndel1 reduces cytoplasmic dynein activity, as shown by Golgi distribution, vesicle trafficking, retrograde transport of dynein, and neuronal migration assays. Palmitoylation assay (metabolic labeling), co-immunoprecipitation, organelle localization assays, neuronal migration assay, time-lapse imaging The EMBO journal High 19927128
2009 Nudel binds to paxillin at nascent adhesions and colocalization is observed in areas of active membrane protrusions. Focal adhesion kinase (FAK) disrupts the Nudel-paxillin interaction in a paxillin-binding-dependent manner. Forced localization of Nudel to all focal contacts markedly strengthened adhesivity, while overexpression of activated FAK caused cell edge shrinkage. Co-immunoprecipitation, fusion protein forced localization, RNAi, cell migration assays, immunofluorescence PLoS biology Medium 19492042
2009 Nudel is required for retrograde axonal transport in DRG neurons; anti-Nudel antibody injection abolishes retrograde transport of membranous organelles and leads to lysosome accumulation in axons and delayed endo-lysosome formation. Microinjection of blocking antibody into cultured DRG neurons, time-lapse microscopy, Golgi and lysosome localization assays Traffic Medium 19522757
2010 Depletion of both NDE1 and NDEL1 causes striking dispersal of Golgi and endocytic organelles and complete loss of dynein from membranes. A substantial portion of NDE1 and NDEL1 is membrane-associated. NDE1 and NDEL1 act upstream of LIS1 in dynein recruitment on membrane: exogenous NDE1 or NDEL1 rescues LIS1 depletion effects on Golgi, while LIS1 only partially rescues loss of NDE1 and NDEL1. siRNA knockdown (single and double), organelle localization imaging, membrane fractionation, epistasis rescue experiments Journal of cell science High 20048338
2010 Nudel/Ndel1 regulates microtubule organization during spindle assembly independently of kinetochore functions. Nudel directly interacts with lamin B to facilitate lamin-B-containing matrix accumulation and assembly on microtubules in a dynein-dependent manner. A novel dynein binding domain within the first 80 amino acids of Nudel was identified that interacts with dynein intermediate chain. Xenopus egg extract spindle assembly assay, immunodepletion, protein binding assays, extensive mutagenesis Nature cell biology / The Journal of biological chemistry High 19198602 21056974
2010 Genetic mosaic analysis (MADM) established that Ndel1 is required cell-autonomously for a specific late step of neuronal migration: entry into the target lamina, distinct from Lis1's role in migration efficiency. Mosaic Analysis with Double Markers (MADM), in vivo sparse clonal analysis in mouse cortex Neuron High 21092859
2011 Cdk5-phosphorylated Ndel1 promotes a high-affinity Lis1/Ndel1/dynein complex that blocks ATP-dependent release of dynein from microtubules, inhibiting processive motility. In adult axons, unphosphorylated Ndel1 inhibits dynein-mediated transport; Cdk5 phosphorylation of Ndel1 releases this inhibition and allows Lis1 to further stimulate cargo transport. RNAi, phosphorylation mutants, organelle transport assays in adult DRG axons, dominant-negative Cdk5 The Journal of neuroscience / Cell reports High 22114287 26166569
2005 NUDEL possesses endooligopeptidase (cysteine protease) activity; mutation of Cys-273 fully abolishes this activity without disrupting secondary structure. DISC1 inhibits NUDEL-oligopeptidase activity in a competitive fashion, and the catalytic site is close to the DISC1-binding site on NUDEL. Site-directed mutagenesis of catalytic Cys-273, enzymatic activity assay, competitive inhibition assay Proceedings of the National Academy of Sciences of the United States of America High 15728732
2010 The endooligopeptidase activity of Ndel1 is functionally important for neurite outgrowth in PC12 cells. Wild-type Ndel1 increases neurite-bearing cells; the catalytically dead mutant Ndel1(C273A) decreases neurite outgrowth; and RNAi depletion of Ndel1 is rescued by enzymatically active Ndel1(WT) but not Ndel1(C273A). RNAi, rescue with wild-type vs. catalytic mutant, neurite outgrowth quantification in PC12 cells Molecular and cellular neurosciences Medium 20462516
2008 Ndel1 directly interacts with PDE4 family members, and the interaction with PDE4D3 is specifically disrupted by PKA phosphorylation at Ser13 of PDE4D3. Ndel1 sequesters EPAC (but not PKA) to form a cAMP signaling complex. Ndel1 self-interaction (dimerization) is stabilized by PDE4 binding. Co-immunoprecipitation, BRET interaction assay in living cells, peptide array mapping, PKA phosphorylation assay Cellular signalling Medium 18845247
2011 PKA phosphorylates NDE1 at threonine-131 (T131) in a DISC1/PDE4-dependent manner. T131 phosphorylation modulates NDE1-LIS1 and NDE1-NDEL1 interactions, as confirmed by homology modeling and experimental binding assays. Mutation of T131 to mimic PKA phosphorylation inhibits neurite outgrowth. In vitro kinase assay, co-immunoprecipitation, homology modeling, neurite outgrowth assay, immunofluorescence The Journal of neuroscience Medium 21677187
2012 Full-length NDEL1 forms dimers, tetramers, and chain-like polymers with a folded-back structure in solution. The C-terminal region, required for interaction with dynein and DISC1, folds back onto the N-terminal coiled-coil domain. NDE1 and NDEL1 can interact directly to form mixed complexes. Negative stain electron microscopy, chemical cross-linking/mass spectrometry, isotope labeling, biophysical characterization The Journal of biological chemistry High 22843697
2012 Nudel directly interacts with misfolded Gβ (mfGβ) and recruits it to cytoplasmic dynein for transport to the centrosome/aggresome. Depletion of Nudel by RNAi reduces dynein-associated mfGβ, impairs aggresome formation, and prolongs the half-life of nascent Gβ. Co-immunoprecipitation, RNAi, half-life measurement, aggresome formation assay Cell research Medium 22430153
2012 Nudel is crucial for in vivo assembly of the WAVE regulatory complex (WRC). Nudel stabilizes the Sra1-Nap1-Abi1 subcomplex through dynamic binding to Sra1 and protects HSPC300 from proteasomal degradation, stimulating HSPC300-WAVE2 complex formation. Depletion of Nudel abolishes WRC-dependent actin polymerization in vitro and Rac1-induced lamellipodial actin networks. Co-immunoprecipitation, RNAi, in vitro actin polymerization assay, cell spreading assay Cell research Medium 22453242
2013 LIS1 is required for mitotic spindle organization via the LIS1-NDEL1-dynein complex. Overexpression of NDEL1-dynein and microtubule stabilization rescues spindle orientation defects in Lis1 mutants, placing NDEL1-dynein downstream of LIS1 in spindle orientation. Lis1 mutant mouse embryonic fibroblasts, time-lapse live cell imaging, rescue experiments, immunofluorescence Human molecular genetics Medium 24030547
2013 Ndel1 and NudCL together regulate retrograde axonal mitochondrial transport. Knocking down both Ndel1 and NudCL almost blocks retrograde mitochondrial transport, while each alone only partially reduces it. LIS1 also interacts with KIF5b (kinesin) and its depletion suppresses mitochondrial motility in both anterograde and retrograde directions. siRNA knockdown (single and double), live imaging of mitochondrial transport in hippocampal neurons, co-immunoprecipitation Traffic Medium 23551859
2016 NDEL1 localizes to the axon initial segment (AIS) via interaction with the scaffold protein Ankyrin-G. Depletion of NDEL1 or LIS1 results in non-polarized trafficking of dendritic cargo through the AIS. NDEL1 facilitates reversal of somatodendritic cargos in the proximal axon via local dynein activation. Co-immunoprecipitation, RNAi, live cargo tracking, immunofluorescence, fractionation Neuron High 26844830
2016 Ndel1 localizes to the subdistal appendage of the mother centriole and suppresses primary cilia formation in proliferating cells by maintaining trichoplein at the mother centriole, thereby sustaining Aurora A activation. Serum starvation induces transient Ndel1 degradation followed by trichoplein disappearance and cilia assembly. Ndel1 acts upstream of the trichoplein-Aurora A pathway. siRNA knockdown, forced expression, immunofluorescence, mouse genetics (Ndel1 hypomorphic mice), co-localization studies The Journal of cell biology High 26880200
2016 Ndel1 interacts with TRIO-associated repeat on actin (Tara), an actin-bundling protein. Loss of Ndel1 or Tara impairs cell migration. Tara overexpression induces accumulation of Ndel1 at the cell periphery co-localizing with F-actin. Co-expression of Ndel1 and Tara causes synergistic increase in F-actin and filopodia formation. Co-immunoprecipitation, RNAi, wound healing and Boyden chamber migration assays, immunofluorescence Scientific reports Medium 27546710
2017 Crystal structure of DISC1 C-terminal tail in complex with Ndel1 binding domain was solved at high resolution. DISC1 regulates Ndel1 kinetochore attachment (but not centrosome localization) during mitosis. Disrupting DISC1/Ndel1 complex formation prolongs mitotic length and causes cell-cycle deficits in human cells and mouse cortical radial glia. X-ray crystallography, co-immunoprecipitation, live cell imaging, mouse in utero electroporation, human forebrain organoids, patient iPSCs Neuron High 29103808
2019 Crystal structure of AnkB in complex with Ndel1 C-terminal coiled-coil (CT-CC) region revealed a stable 5-helix bundle with 2:1 (Ndel1:AnkB) stoichiometry. AnkG is essential for Ndel1 accumulation at the AIS. Cargo sorting at the AIS is disrupted by a peptide designed to block the AnkG/Ndel1 complex. X-ray crystallography, co-immunoprecipitation, live cargo tracking, peptide competition assay Proceedings of the National Academy of Sciences of the United States of America High 31889000
2015 GSK-3β phosphorylates dynein intermediate chain (IC) at conserved residues S87/T88 (IC-1B) and S88/T89 (IC-2C) within the Ndel1-binding domain. This phosphorylation reduces IC interaction with Ndel1. Pharmacological or genetic inhibition of GSK-3β stimulates dynein motility. Co-immunoprecipitation, mass spectrometry, site-directed mutagenesis, in vitro phosphorylation assay, dynein motility assay Traffic Medium 26010407
2015 DBZ (DISC1-binding zinc finger protein) hinders Ndel1 phosphorylation at Thr219 and Ser251. DBZ depletion or expression of double-phosphorylated Ndel1 impairs anterograde transport of Lis1 and DISC1 to neurite tips. This identifies a role for Ndel1 dual-phosphorylation state in regulating anterograde transport. RNAi, phosphomimetic expression, in utero electroporation, immunofluorescence, microtubule elongation assay The Journal of neuroscience Medium 25698733
2017 NDEL1 is specifically S-nitrosylated at Cys203 by neuronal nitric oxide synthase (nNOS) in an NMDA receptor-activity-dependent manner. This S-nitrosylation accelerates dendritic arborization. Disruption of NDEL1 S-nitrosylation mediates impaired dendritic maturation caused by developmental alcohol exposure. Biotin-switch S-nitrosylation assay, site-directed mutagenesis of Cys203, genetic Nos1 deletion, NMDA receptor pharmacology, dendritic morphometry Cerebral cortex Medium 27371763
2011 Ndel1 directly associates with Dynamin 2 (Dyn2) and enhances Dyn2 GTPase activity in its unassembled and assembled forms without promoting oligomerization. Gain and loss of function of Ndel1 recapitulate effects of Dyn2 overexpression or dominant-negative Dyn2 on intracellular localization of GluR1. Co-immunoprecipitation, in vitro GTPase activity assay, gain/loss-of-function experiments, GluR1 localization assay PloS one Medium 21283621
2021 Ndel1 binds directly to keratin subunits through a motif conserved in all intermediate filament proteins and is necessary for robust desmosome-keratin association. Lis1 is required for desmosomal localization of Ndel1 but not for its effects on keratin filaments. Loss of Ndel1 in mouse epidermis results in desmosome defects. Direct binding assay, mouse genetics (conditional KO), immunofluorescence, desmosome morphology analysis Molecular biology of the cell Medium 34319758
2023 The dynein intermediate chain N-terminus (ICN) is a critical evolutionarily conserved hub that interacts with both dynactin and Ndel1. Ndel1 recruits LIS1 to the dynein complex via ICN binding. LIS1 cannot simultaneously bind Ndel1 and dynein, requiring LIS1 to be handed off from Ndel1 to dynein in temporally discrete steps. In vitro, Ndel1 inhibits dynein activation by disfavoring dynein-dynactin-adaptor complex formation and by sequestering Lis1 away from dynein. Phosphomimetic C-terminal domain mutations in Ndel1 increase its ability to inhibit dynein-dynactin-adaptor complex formation. Purified protein binding assays, single-molecule imaging, in vitro reconstitution of dynein-dynactin-adaptor complex, mutagenesis Nature communications / The Journal of biological chemistry High 37086789 37730751
2024 The NDEL1 variant p.R105P disrupts NDEL1 binding to LIS1, impairs neuronal migration, increases leading process length, and disrupts nucleus-centrosome coupling (nucleokinesis). This identifies the critical role of the LIS1-binding interface of NDEL1 in nucleokinesis during cortical development. In utero electroporation (mouse), single-cell RNA sequencing, spatial transcriptomics, biochemical binding assay, patient variant analysis Acta neuropathologica High 38194050

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 NUDEL is a novel Cdk5 substrate that associates with LIS1 and cytoplasmic dynein. Neuron 412 11163260
2003 14-3-3epsilon is important for neuronal migration by binding to NUDEL: a molecular explanation for Miller-Dieker syndrome. Nature genetics 327 12796778
2002 Disrupted-in-Schizophrenia-1 (DISC-1): mutant truncation prevents binding to NudE-like (NUDEL) and inhibits neurite outgrowth. Proceedings of the National Academy of Sciences of the United States of America 326 12506198
2003 DISC1 (Disrupted-In-Schizophrenia 1) is a centrosome-associated protein that interacts with MAP1A, MIPT3, ATF4/5 and NUDEL: regulation and loss of interaction with mutation. Human molecular genetics 314 12812986
2004 Ndel1 operates in a common pathway with LIS1 and cytoplasmic dynein to regulate cortical neuronal positioning. Neuron 307 15473966
1998 The Saccharomyces cerevisiae NDE1 and NDE2 genes encode separate mitochondrial NADH dehydrogenases catalyzing the oxidation of cytosolic NADH. The Journal of biological chemistry 240 9733747
2010 Genetic mosaic dissection of Lis1 and Ndel1 in neuronal migration. Neuron 201 21092859
2007 DISC1 regulates the transport of the NUDEL/LIS1/14-3-3epsilon complex through kinesin-1. The Journal of neuroscience : the official journal of the Society for Neuroscience 197 17202468
2004 Disrupted in Schizophrenia 1 and Nudel form a neurodevelopmentally regulated protein complex: implications for schizophrenia and other major neurological disorders. Molecular and cellular neurosciences 187 14962739
2008 LIS1 and NDEL1 coordinate the plus-end-directed transport of cytoplasmic dynein. The EMBO journal 154 18784752
2006 DISC1-NDEL1/NUDEL protein interaction, an essential component for neurite outgrowth, is modulated by genetic variations of DISC1. Human molecular genetics 146 17035248
2007 Cenp-F links kinetochores to Ndel1/Nde1/Lis1/dynein microtubule motor complexes. Current biology : CB 139 17600710
2005 Complete loss of Ndel1 results in neuronal migration defects and early embryonic lethality. Molecular and cellular biology 133 16107726
2006 NDEL1 phosphorylation by Aurora-A kinase is essential for centrosomal maturation, separation, and TACC3 recruitment. Molecular and cellular biology 124 17060449
2003 Human Nudel and NudE as regulators of cytoplasmic dynein in poleward protein transport along the mitotic spindle. Molecular and cellular biology 119 12556484
2007 NudE and NudEL are required for mitotic progression and are involved in dynein recruitment to kinetochores. The Journal of cell biology 118 17682047
2016 Dynein Regulator NDEL1 Controls Polarized Cargo Transport at the Axon Initial Segment. Neuron 108 26844830
2004 Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein. The Journal of cell biology 108 14970193
2009 An essential role of the aPKC-Aurora A-NDEL1 pathway in neurite elongation by modulation of microtubule dynamics. Nature cell biology 105 19668197
2017 DISC1 Regulates Neurogenesis via Modulating Kinetochore Attachment of Ndel1/Nde1 during Mitosis. Neuron 102 29103808
2010 Functional interplay between LIS1, NDE1 and NDEL1 in dynein-dependent organelle positioning. Journal of cell science 102 20048338
2009 Requirement for Nudel and dynein for assembly of the lamin B spindle matrix. Nature cell biology 97 19198602
2008 Elucidating the relationship between DISC1, NDEL1 and NDE1 and the risk for schizophrenia: evidence of epistasis and competitive binding. Human molecular genetics 93 18469341
2011 A Cdk5-dependent switch regulates Lis1/Ndel1/dynein-driven organelle transport in adult axons. The Journal of neuroscience : the official journal of the Society for Neuroscience 92 22114287
2009 Distinct dose-dependent cortical neuronal migration and neurite extension defects in Lis1 and Ndel1 mutant mice. The Journal of neuroscience : the official journal of the Society for Neuroscience 88 20007476
1995 An unusual mosaic protein with a protease domain, encoded by the nudel gene, is involved in defining embryonic dorsoventral polarity in Drosophila. Cell 88 7671306
2005 NudEL targets dynein to microtubule ends through LIS1. Nature cell biology 87 15965467
2005 Recruitment of katanin p60 by phosphorylated NDEL1, an LIS1 interacting protein, is essential for mitotic cell division and neuronal migration. Human molecular genetics 85 16203747
2010 Evidence of statistical epistasis between DISC1, CIT and NDEL1 impacting risk for schizophrenia: biological validation with functional neuroimaging. Human genetics 81 20084519
2004 A NUDEL-dependent mechanism of neurofilament assembly regulates the integrity of CNS neurons. Nature cell biology 79 15208636
2005 Nudel contributes to microtubule anchoring at the mother centriole and is involved in both dynein-dependent and -independent centrosomal protein assembly. Molecular biology of the cell 77 16291865
2013 LIS1 controls mitosis and mitotic spindle organization via the LIS1-NDEL1-dynein complex. Human molecular genetics 76 24030547
2007 Nudel modulates kinetochore association and function of cytoplasmic dynein in M phase. Molecular biology of the cell 74 17494871
2008 Lis1 and Ndel1 influence the timing of nuclear envelope breakdown in neural stem cells. The Journal of cell biology 73 18809722
2011 PKA phosphorylation of NDE1 is DISC1/PDE4 dependent and modulates its interaction with LIS1 and NDEL1. The Journal of neuroscience : the official journal of the Society for Neuroscience 72 21677187
2007 The structure of the coiled-coil domain of Ndel1 and the basis of its interaction with Lis1, the causal protein of Miller-Dieker lissencephaly. Structure (London, England : 1993) 72 17997972
2008 Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation. The Journal of cell biology 69 18347064
2005 Inhibition of NUDEL (nuclear distribution element-like)-oligopeptidase activity by disrupted-in-schizophrenia 1. Proceedings of the National Academy of Sciences of the United States of America 66 15728732
2008 Nudel binds Cdc42GAP to modulate Cdc42 activity at the leading edge of migrating cells. Developmental cell 64 18331715
2016 Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway. The Journal of cell biology 62 26880200
2000 The nudel protease of Drosophila is required for eggshell biogenesis in addition to embryonic patterning. Developmental biology 55 10625559
2015 Stress-Induced CDK5 Activation Disrupts Axonal Transport via Lis1/Ndel1/Dynein. Cell reports 54 26166569
2016 NDE1 and NDEL1 from genes to (mal)functions: parallel but distinct roles impacting on neurodevelopmental disorders and psychiatric illness. Cellular and molecular life sciences : CMLS 52 27742926
2005 Subcellular targeting of DISC1 is dependent on a domain independent from the Nudel binding site. Molecular and cellular neurosciences 52 15797709
2010 Identification of a novel dynein binding domain in nudel essential for spindle pole organization in Xenopus egg extract. The Journal of biological chemistry 51 21056974
2015 GSK-3β Phosphorylation of Cytoplasmic Dynein Reduces Ndel1 Binding to Intermediate Chains and Alters Dynein Motility. Traffic (Copenhagen, Denmark) 47 26010407
1998 Role of Nudel protease activation in triggering dorsoventral polarization of the Drosophila embryo. Development (Cambridge, England) 43 9735365
2008 Ndel1 controls the dynein-mediated transport of vimentin during neurite outgrowth. The Journal of biological chemistry 42 18303022
2009 Nudel and FAK as antagonizing strength modulators of nascent adhesions through paxillin. PLoS biology 41 19492042
2009 Ndel1 palmitoylation: a new mean to regulate cytoplasmic dynein activity. The EMBO journal 41 19927128
2007 Expression patterns of LIS1, dynein and their interaction partners dynactin, NudE, NudEL and NudC in human gliomas suggest roles in invasion and proliferation. Acta neuropathologica 41 17221205
2001 NudE-L, a novel Lis1-interacting protein, belongs to a family of vertebrate coiled-coil proteins. Mechanisms of development 41 11231056
2013 Distinct functions of nuclear distribution proteins LIS1, Ndel1 and NudCL in regulating axonal mitochondrial transport. Traffic (Copenhagen, Denmark) 38 23551859
2013 NDE1 and NDEL1: twin neurodevelopmental proteins with similar 'nature' but different 'nurture'. Biomolecular concepts 38 24093049
2019 NFAT1-Mediated Regulation of NDEL1 Promotes Growth and Invasion of Glioma Stem-like Cells. Cancer research 36 30940662
2012 The mitosis and neurodevelopment proteins NDE1 and NDEL1 form dimers, tetramers, and polymers with a folded back structure in solution. The Journal of biological chemistry 36 22843697
2010 Assessing the role of endooligopeptidase activity of Ndel1 (nuclear-distribution gene E homolog like-1) in neurite outgrowth. Molecular and cellular neurosciences 34 20462516
2008 Ndel1 alters its conformation by sequestering cAMP-specific phosphodiesterase-4D3 (PDE4D3) in a manner that is dynamically regulated through Protein Kinase A (PKA). Cellular signalling 34 18845247
2010 The essential role of LIS1, NDEL1 and Aurora-A in polarity formation and microtubule organization during neurogensis. Cell adhesion & migration 33 20168084
1996 The maternal nudel protein of Drosophila has two distinct roles important for embryogenesis. Genetics 33 8844153
2013 Plasma Ndel1 enzyme activity is reduced in patients with schizophrenia--a potential biomarker? Journal of psychiatric research 32 23388542
2009 Nudel promotes axonal lysosome clearance and endo-lysosome formation via dynein-mediated transport. Traffic (Copenhagen, Denmark) 32 19522757
2011 Ndel1, Nudel (Noodle): flexible in the cell? Cytoskeleton (Hoboken, N.J.) 30 21948775
2022 miR-103-3p targets Ndel1 to regulate neural stem cell proliferation and differentiation. Neural regeneration research 25 34269216
2023 Conserved roles for the dynein intermediate chain and Ndel1 in assembly and activation of dynein. Nature communications 23 37730751
2015 Increased expression of NDEL1 and MBP genes in the peripheral blood of antipsychotic-naïve patients with first-episode psychosis. European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology 22 26476704
2009 Opposing effects of Ndel1 and alpha1 or alpha2 on cytoplasmic dynein through competitive binding to Lis1. Journal of cell science 22 19622634
2016 Regulation of the actin cytoskeleton by the Ndel1-Tara complex is critical for cell migration. Scientific reports 21 27546710
2015 DBZ regulates cortical cell positioning and neurite development by sustaining the anterograde transport of Lis1 and DISC1 through control of Ndel1 dual-phosphorylation. The Journal of neuroscience : the official journal of the Society for Neuroscience 20 25698733
2023 Ndel1 disfavors dynein-dynactin-adaptor complex formation in two distinct ways. The Journal of biological chemistry 19 37086789
2022 Nde1 and Ndel1: Outstanding Mysteries in Dynein-Mediated Transport. Frontiers in cell and developmental biology 19 35493069
2014 The expression and roles of Nde1 and Ndel1 in the adult mammalian central nervous system. Neuroscience 18 24785679
2004 Expression of NUDEL in manchette and its implication in spermatogenesis. FEBS letters 18 15147871
2000 Biochemical defects of mutant nudel alleles causing early developmental arrest or dorsalization of the Drosophila embryo. Genetics 18 10628985
2020 Arsenic trioxide disturbs the LIS1/NDEL1/dynein microtubule dynamic complex by disrupting the CLIP170 zinc finger in head and neck cancer. Toxicology and applied pharmacology 17 32717241
2019 Mechanistic insights into the interactions of dynein regulator Ndel1 with neuronal ankyrins and implications in polarity maintenance. Proceedings of the National Academy of Sciences of the United States of America 17 31889000
2016 Ndel1 and Reelin Maintain Postnatal CA1 Hippocampus Integrity. The Journal of neuroscience : the official journal of the Society for Neuroscience 17 27307241
2012 Nudel is crucial for the WAVE complex assembly in vivo by selectively promoting subcomplex stability and formation through direct interactions. Cell research 16 22453242
2019 Ndel1 oligopeptidase activity as a potential biomarker of early stages of schizophrenia. Schizophrenia research 15 30857875
2018 Oligopeptidases activity in bipolar disorder: Ndel1 and angiotensin I converting enzyme. Journal of affective disorders 14 30321766
2014 NDEL1 was decreased in the CA3 region but increased in the hippocampal blood vessel network during the spontaneous seizure period after pilocarpine-induced status epilepticus. Neuroscience 14 24680936
2012 Misfolded Gβ is recruited to cytoplasmic dynein by Nudel for efficient clearance. Cell research 13 22430153
2016 Genome-wide investigation of schizophrenia associated plasma Ndel1 enzyme activity. Schizophrenia research 12 26851141
2012 Ndel1-derived peptides modulate bidirectional transport of injected beads in the squid giant axon. Biology open 12 23213412
2021 Behavioral Deficits in Mice with Postnatal Disruption of Ndel1 in Forebrain Excitatory Neurons: Implications for Epilepsy and Neuropsychiatric Disorders. Cerebral cortex communications 11 33615226
2015 Protein-Protein and Peptide-Protein Interactions of NudE-Like 1 (Ndel1): A Protein Involved in Schizophrenia. Current protein & peptide science 11 25961396
2021 Expression and function of Ndel1 during the differentiation of neural stem cells induced by hippocampal exosomesticle. Stem cell research & therapy 10 33422130
2020 Role of NDEL1 and VEGF/VEGFR-2 in Mouse Hippocampus After Status Epilepticus. ASN neuro 10 32423231
2005 Cloning and characterization of the human and rabbit NUDEL-oligopeptidase promoters and their negative regulation. Biochimica et biophysica acta 10 16005531
2002 Evidence for a glycosaminoglycan on the nudel protein important for dorsoventral patterning of the drosophila embryo. Developmental dynamics : an official publication of the American Association of Anatomists 10 11984873
2024 Novel lissencephaly-associated NDEL1 variant reveals distinct roles of NDE1 and NDEL1 in nucleokinesis and human cortical malformations. Acta neuropathologica 9 38194050
2017 Developmental Alcohol Exposure Impairs Activity-Dependent S-Nitrosylation of NDEL1 for Neuronal Maturation. Cerebral cortex (New York, N.Y. : 1991) 9 27371763
2016 Status epilepticus stimulates NDEL1 expression via the CREB/CRE pathway in the adult mouse brain. Neuroscience 9 27298008
2020 Reelin Improves Cognition and Extends the Lifespan of Mutant Ndel1 Mice with Postnatal CA1 Hippocampus Deterioration. Cerebral cortex (New York, N.Y. : 1991) 8 32328622
2020 Evaluation of NDEL1 oligopeptidase activity in blood and brain in an animal model of schizophrenia: effects of psychostimulants and antipsychotics. Scientific reports 8 33116174
2018 Nudel involvement in the high-glucose-induced epithelial-mesenchymal transition of tubular epithelial cells. American journal of physiology. Renal physiology 8 30539652
2023 Conserved Roles for the Dynein Intermediate Chain and Ndel1 in Assembly and Activation of Dynein. bioRxiv : the preprint server for biology 7 36711700
2022 LIS1 and NDEL1 Regulate Axonal Trafficking of Mitochondria in Mature Neurons. Frontiers in molecular neuroscience 7 35465088
2021 Roles for Ndel1 in keratin organization and desmosome function. Molecular biology of the cell 6 34319758
2011 The cytoskeletal protein Ndel1 regulates dynamin 2 GTPase activity. PloS one 6 21283621

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