Affinage

NDEL1

Nuclear distribution protein nudE-like 1 · UniProt Q9GZM8

Length
345 aa
Mass
38.4 kDa
Annotated
2026-04-29
100 papers in source corpus 56 papers cited in narrative 56 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NDEL1 is a coiled-coil scaffold protein that functions as a central regulator of cytoplasmic dynein activity, coordinating microtubule-based transport, mitotic spindle organization, neuronal migration, and centrosome function. NDEL1 recruits LIS1 to the dynein intermediate chain and controls a timed handoff of LIS1 to dynein: unphosphorylated NDEL1 inhibits dynein activation by sequestering LIS1 and disfavoring dynein–dynactin–adaptor complex formation, while phosphorylation by CDK5, CDK1, Aurora A, or Erk1/2—counteracted by PP4c dephosphorylation—modulates its binding affinities for dynein, LIS1, and effectors such as katanin p60, thereby tuning dynein processivity, centrosome maturation, kinetochore dynein recruitment, nuclear envelope breakdown, and cargo-selective axonal transport (PMID:37730751, PMID:11163260, PMID:17060449, PMID:18347064, PMID:15473966). Beyond dynein regulation, NDEL1 directly binds intermediate filament subunits (neurofilament light chain, keratins, vimentin) to promote their assembly and transport, interacts with Ankyrin-G at the axon initial segment to enforce somatodendritic cargo sorting, suppresses ciliogenesis via the trichoplein–Aurora A pathway at the mother centriole, and possesses intrinsic oligopeptidase activity required for neurite outgrowth (PMID:15208636, PMID:26844830, PMID:26880200, PMID:15728732). A de novo somatic mosaic NDEL1 variant (p.R105P) that disrupts LIS1 binding causes severe neuronal migration defects, establishing NDEL1 as a gene in which pathogenic variants impair cortical development (PMID:38194050).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 2000 High

    Establishing that NDEL1 is a CDK5 substrate that physically links LIS1 to cytoplasmic dynein answered the foundational question of how CDK5 signaling connects to the dynein transport machinery in neurons.

    Evidence Co-immunoprecipitation, in vitro kinase assay, and immunofluorescence in neurons treated with CDK5 inhibitor

    PMID:11163260

    Open questions at the time
    • Precise phosphorylation sites on NDEL1 and their individual functional consequences were not mapped
    • Whether NDEL1 acts upstream or downstream of LIS1 in dynein regulation was unresolved
  2. 2003 High

    Identifying CDK1 and Erk1/2 as additional NDEL1 kinases and showing that M-phase phosphorylation increases LIS1 binding and regulates poleward dynein transport revealed that NDEL1 phosphorylation is a cell-cycle switch for dynein function, while 14-3-3ε was shown to maintain NDEL1 phosphorylation state in vivo.

    Evidence In vitro kinase assays, phosphomimetic/non-phosphorylatable mutants, dominant-negative overexpression (CDK1/Erk1/2); 14-3-3ε knockout mice with NDEL1/LIS1 mislocalization

    PMID:12556484 PMID:12796778

    Open questions at the time
    • How phosphorylation by different kinases is coordinated temporally was unknown
    • Whether 14-3-3ε protection of phospho-NDEL1 is direct or indirect was not biochemically resolved
  3. 2004 High

    Demonstrating that NDEL1 acts upstream of LIS1 in dynein regulation during cortical neuronal migration, and that it mediates dynein-dependent membrane organelle transport and neurofilament assembly, broadened NDEL1's role from a simple adaptor to a multifunctional scaffold.

    Evidence In vivo RNAi with LIS1 overexpression rescue (neuronal positioning); binding-defective mutant overexpression causing Golgi/lysosome dispersal; direct NF-L binding assay and in vitro NF assembly

    PMID:14970193 PMID:15208636 PMID:15473966

    Open questions at the time
    • The molecular mechanism by which NDEL1 promotes NF polymerization was not defined
    • Whether NDEL1's organelle transport and NF roles are dynein-dependent or independent was unclear
  4. 2005 High

    Discovery of NDEL1's intrinsic oligopeptidase activity (Cys273-dependent), its interaction with katanin p60 regulated by CDK5 phosphorylation, and its essential role in embryonic viability revealed unexpected enzymatic and microtubule-severing regulatory functions.

    Evidence In vitro peptidase assay with C273A mutagenesis; CDK5-dependent Co-IP of katanin p60; Ndel1 knockout (lethal) and hypomorph mice with migration defects

    PMID:15728732 PMID:16107726 PMID:16203747

    Open questions at the time
    • Physiological substrates of the oligopeptidase activity were not identified
    • Whether katanin p60 regulation and oligopeptidase activity represent independent or linked NDEL1 functions was unknown
  5. 2005 High

    Showing that yeast NudEL homolog targets dynein to microtubule plus ends through LIS1 established an evolutionarily conserved mechanism for NDEL1 in dynein positioning, while centrosome studies revealed NDEL1 as a mother centriole protein coordinating dynein-dependent and -independent centrosomal targeting.

    Evidence Yeast Ndl1 null with live imaging of dynein at MT ends; RNAi and deletion mutant analysis of centrosomal protein recruitment

    PMID:15965467 PMID:16291865

    Open questions at the time
    • The transient nature of NDEL1 at plus ends was not mechanistically explained
    • How NDEL1 mediates dynein-independent targeting of pericentrin/γ-tubulin was not resolved
  6. 2006 High

    Aurora A phosphorylation of NDEL1 at Ser251 was shown to be required for centrosome maturation and TACC3 recruitment, followed by NDEL1 degradation, revealing a phosphorylation-triggered lifecycle for NDEL1 at mitotic centrosomes.

    Evidence In vitro kinase assay, phospho-specific antibodies, phosphomimetic/dead mutants, siRNA with defined centrosome phenotype

    PMID:17060449

    Open questions at the time
    • The ubiquitin ligase responsible for Aurora A-phosphorylated NDEL1 degradation was not identified
    • Whether Ser251 phosphorylation affects dynein binding independently of centrosome maturation was untested
  7. 2007 High

    Multiple studies converged to establish NDEL1 as essential for kinetochore dynein recruitment: NDEL1 localizes to kinetochores in a CENP-F/mitosin-dependent manner, binds dynein via intermediate and light chains, and stabilizes kinetochore dynein against microtubule-dependent stripping; DISC1 was shown to bridge NDEL1 and LIS1 through independent binding domains.

    Evidence Co-IP defining dynein IC/LC binding; antibody microinjection displacing dynein from kinetochores; CENP-F-dependent localization; DISC1–NDEL1–LIS1 domain mapping; crystal structure of NDEL1 coiled-coil homodimer

    PMID:14962739 PMID:17494871 PMID:17600710 PMID:17682047 PMID:17997972

    Open questions at the time
    • How NDEL1 and NDE1 divide kinetochore functions was not fully resolved
    • Whether DISC1-regulated kinetochore attachment of NDEL1 affects chromosome segregation fidelity was untested
  8. 2008 High

    In vitro reconstitution showed that LIS1 blocks dynein motility on microtubules and NDEL1 releases this block, establishing the core mechanistic principle; PP4c was identified as the phosphatase opposing CDK1 on NDEL1; and NDEL1 was found to regulate Cdc42 activity and cell migration via Cdc42GAP sequestration.

    Evidence In vitro microtubule motility assay with purified proteins; in vitro PP4c phosphatase assay plus PP4c KO mice; Cdc42GAP competition binding and Cdc42 activity assays

    PMID:18331715 PMID:18347064 PMID:18784752

    Open questions at the time
    • The in vitro motility assay used bead-based dynein, not fully reconstituted dynein–dynactin–adaptor complexes
    • Whether Cdc42GAP regulation is dynein-dependent or represents a fully independent NDEL1 function was unclear
  9. 2009 High

    NDEL1 was shown to be required for dynein-dependent lamin B spindle matrix assembly, for prophase nuclear envelope invagination (regulated by its phosphorylation state), and for retrograde axonal organelle transport; palmitoylation was identified as a lipid modification that negatively regulates NDEL1–dynein interaction.

    Evidence Xenopus egg extract spindle assay with immunodepletion; RNAi and phospho-mutant analysis of NE dynamics; antibody microinjection blocking axonal transport; acyl-RAC palmitoylation assay

    PMID:18809722 PMID:19198602 PMID:19522757 PMID:19927128

    Open questions at the time
    • The palmitoyltransferase responsible for NDEL1 palmitoylation was not identified
    • How palmitoylation and phosphorylation are coordinated was unknown
  10. 2010 High

    Genetic mosaic analysis demonstrated that NDEL1 is cell-autonomously required for a specific late step of neuronal migration (laminar entry), distinct from LIS1's role, while biochemical mapping identified a novel dynein-binding domain in NDEL1's first 80 residues essential for spindle pole organization.

    Evidence MADM in mouse cortex with single-cell resolution; Xenopus extract spindle assay with mutagenesis and immunodepletion rescue

    PMID:21056974 PMID:21092859

    Open questions at the time
    • The molecular basis for NDEL1's unique role in terminal translocation versus general migration speed was not defined
    • Whether the N-terminal dynein-binding domain and the C-terminal dynein-interacting region function cooperatively was untested
  11. 2011 High

    CDK5 phosphorylation was shown to switch NDEL1 from a dynein inhibitor to a facilitator in adult axons, revealing that NDEL1's basal state is inhibitory and phosphorylation licenses dynein-dependent transport.

    Evidence Phosphomimetic/dead mutants, dominant-negative CDK5, live imaging of acidic organelle transport in adult DRG neurons

    PMID:22114287

    Open questions at the time
    • Whether this phospho-switch mechanism applies to all dynein cargoes or is cargo-specific was unknown
    • The structural basis for how phosphorylation relieves NDEL1's inhibitory conformation was not determined
  12. 2012 High

    Structural studies revealed that NDEL1 forms dimers/tetramers with a folded-back architecture where the C-terminal domain contacts the N-terminal coiled-coil, mediating dynein and DISC1 interactions; functionally, NDEL1 was shown to act as a cargo receptor for misfolded proteins (Gβ) directing them to aggresomes, and to promote WAVE regulatory complex assembly for actin polymerization.

    Evidence Negative-stain EM and crosslinking-MS; Co-IP with aggresome formation assay; Co-IP with in vitro actin polymerization assay

    PMID:22430153 PMID:22453242 PMID:22843697

    Open questions at the time
    • Whether the folded-back conformation is the auto-inhibited state relieved by phosphorylation was not tested
    • The physiological relevance of WAVE complex regulation versus dynein regulation was not compared
  13. 2015 High

    Mechanistic studies showed that elevated CDK5 activity creates a high-affinity LIS1/NDEL1/dynein complex that paradoxically blocks processive motility by preventing ATP-dependent dynein release from microtubules, while GSK-3β phosphorylation of dynein IC reduces IC–NDEL1 interaction, providing an additional regulatory input.

    Evidence In vitro dynein motility assay combined with CDK5 activation/inhibition; in vitro kinase assay with MS-identified IC phosphosites plus dynein motility measurement

    PMID:26010407 PMID:26166569

    Open questions at the time
    • The dose-response relationship between CDK5 activity level and dynein processivity in vivo was not established
    • How GSK-3β and CDK5 phosphorylation events are integrated at individual dynein complexes was unknown
  14. 2016 High

    NDEL1 was localized to the axon initial segment via Ankyrin-G interaction, where it activates local dynein to enforce somatodendritic cargo sorting; independently, NDEL1 was found to suppress primary ciliogenesis at the mother centriole by stabilizing trichoplein through the trichoplein–Aurora A pathway.

    Evidence siRNA KD with live-cell cargo sorting; Co-IP with AnkG; siRNA plus epistasis rescue (trichoplein/KCTD17); Ndel1-hypomorphic mice with cilia phenotype

    PMID:26844830 PMID:26880200

    Open questions at the time
    • Whether AIS cargo sorting and ciliogenesis suppression are coordinated or fully independent NDEL1 functions was unknown
    • The mechanism by which NDEL1 stabilizes trichoplein was not biochemically defined
  15. 2017 High

    The crystal structure of the DISC1–NDEL1 complex revealed the structural basis of their interaction and showed that DISC1 regulates NDEL1 kinetochore attachment but not centrosome localization; disrupting this complex causes mitotic deficits in human cells and mouse cortex.

    Evidence X-ray crystallography, mutagenesis, live-cell mitotic imaging, in utero electroporation, human forebrain organoids

    PMID:29103808

    Open questions at the time
    • Whether DISC1 regulation of NDEL1 at kinetochores is phosphorylation-dependent was not tested
    • The relationship between DISC1–NDEL1 mitotic function and psychiatric disease pathophysiology was not mechanistically defined
  16. 2019 High

    The crystal structure of Ankyrin-B in complex with NDEL1 C-terminal coiled-coil defined a 5-helix bundle with 2:1 stoichiometry; a blocking peptide disrupting AnkG–NDEL1 interaction impaired cargo sorting at the AIS, providing structural validation of the AIS sorting mechanism.

    Evidence X-ray crystallography, Co-IP, inhibitory peptide, cargo sorting assay

    PMID:31889000

    Open questions at the time
    • Whether AnkG–NDEL1 interaction is regulated by phosphorylation or other PTMs was not addressed
    • The identity of all cargoes sorted by this mechanism was not catalogued
  17. 2023 High

    Reconstitution with purified components resolved the core paradox of NDEL1 function: NDEL1 inhibits dynein activation by disfavoring dynein–dynactin–adaptor complex formation and sequestering LIS1, requiring a temporally controlled handoff of LIS1 from NDEL1 to dynein for productive activation.

    Evidence Purified protein binding assays, single-molecule imaging, mutagenesis across two independent studies

    PMID:37086789 PMID:37730751

    Open questions at the time
    • The cellular signal or factor that triggers LIS1 handoff from NDEL1 to dynein in vivo was not identified
    • Whether phosphorylation directly controls the handoff step was not reconstituted
  18. 2024 High

    A de novo somatic mosaic NDEL1 variant (p.R105P) was identified in a patient and shown to disrupt LIS1 binding and impair neuronal migration and nucleokinesis, establishing NDEL1 as a gene in which pathogenic variants cause cortical malformation.

    Evidence Patient variant identification, in utero electroporation, Co-IP showing disrupted LIS1 binding, live imaging of migration and nucleus–centrosome coupling

    PMID:38194050

    Open questions at the time
    • Whether other NDEL1 variants contribute to lissencephaly spectrum disorders remains to be screened
    • The full clinical phenotype associated with mosaic NDEL1 variants is based on a single case

Open questions

Synthesis pass · forward-looking unresolved questions
  • The cellular trigger that controls the timed handoff of LIS1 from NDEL1 to dynein in vivo, the physiological substrates of NDEL1's oligopeptidase activity, and how NDEL1's diverse functions (dynein regulation, intermediate filament binding, ciliogenesis suppression, Cdc42 regulation, WAVE complex assembly) are spatiotemporally coordinated remain unresolved.
  • No in vivo reconstitution of the LIS1 handoff mechanism exists
  • Oligopeptidase substrates have never been identified in cells
  • A unified structural model integrating all NDEL1 binding interfaces is lacking

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 6 GO:0098772 molecular function regulator activity 4 GO:0008092 cytoskeletal protein binding 3 GO:0016787 hydrolase activity 2
Localization
GO:0005815 microtubule organizing center 4 GO:0005694 chromosome 3 GO:0005856 cytoskeleton 2 GO:0005886 plasma membrane 2 GO:0005829 cytosol 1
Pathway
R-HSA-1640170 Cell Cycle 7 R-HSA-1266738 Developmental Biology 4 R-HSA-5653656 Vesicle-mediated transport 4 R-HSA-162582 Signal Transduction 3 R-HSA-1852241 Organelle biogenesis and maintenance 2
Partners
ANK3CENPFDISC1DYNC1I1KATNA1LIS1LMNB1TACC3
Complex memberships
DISC1-NDEL1-LIS1LIS1-NDEL1-dyneinNDEL1-14-3-3ε

Evidence

Reading pass · 56 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 NUDEL (NDEL1) is a substrate of Cdk5 kinase and interacts with LIS1 and cytoplasmic dynein; it is enriched at centrosomes and neuronal growth cones, and inhibition of Cdk5 modifies NUDEL distribution in neurons and affects neuritic morphology. Co-immunoprecipitation, in vitro kinase assay, immunofluorescence localization, Cdk5 inhibitor treatment Neuron High 11163260
2003 14-3-3epsilon binds to CDK5/p35-phosphorylated NUDEL and this binding maintains NUDEL phosphorylation; deficiency of 14-3-3epsilon causes mislocalization of NUDEL and LIS1, consistent with reduction of cytoplasmic dynein function. Co-immunoprecipitation, mouse knockout genetics, immunofluorescence localization Nature genetics High 12796778
2002 DISC1 interacts with NUDEL (NDEL1); the disease mutant truncated form of DISC1 fails to bind NUDEL, and expression of mutant DISC1 in PC12 cells reduces neurite extension. Yeast two-hybrid, co-immunoprecipitation, PC12 neurite outgrowth assay Proceedings of the National Academy of Sciences of the United States of America High 12506198
2004 Ndel1 positively regulates dynein activity by facilitating the interaction between LIS1 and dynein; loss of Ndel1 in developing neocortex impairs neuronal positioning and causes uncoupling of the centrosome and nucleus. Overexpression of LIS1 partially rescues Ndel1 RNAi positioning defects, placing Ndel1 upstream of LIS1 in the dynein regulatory pathway. RNAi in vivo (developing neocortex), genetic epistasis (LIS1 overexpression rescue), co-immunoprecipitation Neuron High 15473966
2010 Mosaic analysis reveals that Ndel1 is required cell-autonomously for a specific late step of neuronal migration: entry into the target lamina, distinct from LIS1's role in regulating migration efficiency. Mosaic analysis with double markers (MADM) in mouse, sparse labeling of mutant cells Neuron High 21092859
2007 DISC1 regulates the transport of the NUDEL/LIS1/14-3-3epsilon complex into axons through direct interaction with kinesin-1 heavy chain, acting as a cargo receptor for anterograde axonal transport; knockdown of DISC1 inhibits accumulation of NUDEL, LIS1, and 14-3-3epsilon at axons and impairs axon elongation. Co-immunoprecipitation, RNAi knockdown, immunofluorescence, axon elongation assays The Journal of neuroscience High 17202468
2004 DISC1 interacts with Nudel through a leucine zipper domain; Nudel bridges DISC1 and Lis1 to form a trimolecular complex, with the DISC1-interaction domain on Nudel being independent of the Lis1 binding site. Co-immunoprecipitation, yeast two-hybrid, deletion mapping Molecular and cellular neurosciences High 14962739
2008 LIS1 suppresses the motility of cytoplasmic dynein on microtubules, while NDEL1 releases this blocking effect of LIS1 on dynein; LIS1, cytoplasmic dynein, and MT fragments co-migrate anterogradely, and cytoplasmic dynein forms a complex with LIS1, tubulins, and kinesin-1. In vitro microtubule motility assay, co-immunoprecipitation, immunoabsorption, live imaging The EMBO journal High 18784752
2007 Cenp-F interacts with Ndel1 and Nde1; Ndel1, Nde1, and Lis1 localize to kinetochores in a Cenp-F-dependent manner. Suppression of Ndel1 causes chromosome malaligments not detected by the spindle checkpoint, leading to lagging chromosomes in anaphase. Co-immunoprecipitation, siRNA knockdown, immunofluorescence, live-cell imaging Current biology High 17600710
2005 Complete loss of Ndel1 causes embryonic lethality at peri-implantation; compound hypomorphs show neuronal migration defects; Ndel1 and Lis1 act in a common pathway to regulate dynein but each has distinct roles in microtubule organization. Rescue experiments in MEFs and granule cells by overexpressing LIS1, NDEL1, or NDE1 confirm the common pathway. Targeted gene disruption (knockout and hypomorph), genetic rescue overexpression, MEF microtubule assays, granule cell migration assays Molecular and cellular biology High 16107726
2006 Aurora-A phosphorylates NDEL1 at Ser251 at mitotic entry; this phosphorylation is required for centrosomal maturation, separation, and TACC3 recruitment; Aurora-A-phosphorylated NDEL1 is subsequently degraded via ubiquitination. NDEL1 is required for centrosome targeting of TACC3 through direct interaction. In vitro kinase assay, phospho-specific antibodies, siRNA knockdown, phosphomimetic/phospho-dead mutants, co-immunoprecipitation Molecular and cellular biology High 17060449
2003 Nudel is specifically phosphorylated in M phase by Cdc2 (Cdk1) and Erk1/2; phosphorylation regulates cell-cycle-dependent distribution by increasing its dissociation from the MTOC; phosphorylated Nudel binds Lis1 more efficiently; a Nudel mutant incapable of binding Lis1 impairs poleward dynein movement and kinetochore protein transport to spindle poles. In vitro kinase assay (Cdc2, Erk1/2), phosphomimetic/non-phosphorylatable mutants, immunofluorescence, dominant-negative overexpression, microtubule motor transport assays Molecular and cellular biology High 12556484
2007 NudE and NudEL each localize to mitotic kinetochores before dynein; dynein interacts with NudE through the dynein intermediate and light chains (not the motor domain); inhibition of NudE and NudEL displaces dynein and dynactin from kinetochores and causes metaphase arrest. Antibody microinjection, co-immunoprecipitation, immunofluorescence, live-cell imaging The Journal of cell biology High 17682047
2004 Nudel regulates dynein-mediated membrane transport; overexpression of a Nudel mutant defective in Lis1 or dynein heavy chain binding causes dispersal of dynein-dependent membranous organelles; silencing Nudel causes Golgi fragmentation and cell death. Overexpression of binding-defective mutants, RNAi, time-lapse microscopy of lysosomes The Journal of cell biology High 14970193
2016 NDEL1 localizes to the axon initial segment (AIS) via interaction with the scaffold protein Ankyrin-G; depletion of NDEL1 or LIS1 results in non-polarized trafficking of dendritic cargo through the AIS, establishing NDEL1 as a regulator of somatodendritic cargo sorting at the AIS via local dynein activation. siRNA knockdown, live-cell imaging, co-immunoprecipitation, immunofluorescence Neuron High 26844830
2009 An aPKC-Aurora A-NDEL1 pathway regulates microtubule organization during neurite extension; aPKC phosphorylates Aurora A at Thr287, which facilitates Aurora A activation and phosphorylation of NDEL1 at S251, reducing microtubule emanation frequency from the MTOC during neurite extension. In vitro kinase assay, phosphomimetic/dead mutants, live imaging of microtubule dynamics (EB1-GFP), siRNA knockdown Nature cell biology High 19668197
2010 NDE1 and NDEL1 depletion causes Golgi dispersal and complete loss of dynein from membranes; NDE1 and NDEL1 act upstream of LIS1 in dynein recruitment to membranes; exogenous NDE1 or NDEL1 rescues LIS1 depletion effects on Golgi, but LIS1 only partially rescues loss of NDE1/NDEL1. siRNA knockdown, organelle localization by immunofluorescence, membrane fractionation, rescue experiments Journal of cell science High 20048338
2017 Crystal structure of DISC1 C-terminal tail in complex with the Ndel1 binding domain was solved; DISC1 regulates Ndel1's kinetochore attachment but not its centrosome localization during mitosis; disrupting DISC1/Ndel1 complex formation prolongs mitotic length and causes cell-cycle deficits in human cells and embryonic mouse cortex. X-ray crystallography, co-immunoprecipitation, live-cell imaging of mitosis, in utero electroporation, human forebrain organoids Neuron High 29103808
2009 Nudel is required for dynein-dependent assembly of the lamin B spindle matrix; Nudel interacts directly with lamin B to facilitate accumulation of lamin-B-containing matrix on microtubules; perturbing Nudel or dynein inhibits lamin B matrix assembly and causes lack of spindle pole focusing. Xenopus egg extract spindle assembly assay, immunodepletion, direct binding assay, spindle imaging Nature cell biology High 19198602
2011 In adult axons, Cdk5 phosphorylation of Ndel1 switches it from an inhibitor to a facilitator of dynein-mediated organelle transport; unphosphorylated Ndel1 inhibits dynein transport capacity, while phosphorylation by Cdk5 relieves this inhibition and allows LIS1 to further stimulate dynein transport. RNAi, overexpression of phosphorylation-site mutants, dominant-negative Cdk5, live imaging of acidic organelles in adult rat DRG axons The Journal of neuroscience High 22114287
2005 Phosphorylation of NDEL1 by Cdk5 facilitates interaction between NDEL1 and katanin p60 microtubule-severing protein; NDEL1 regulates distribution of katanin p60 to the centrosome; loss of NDEL1 causes abnormal nuclear accumulation of p60 and impaired neuronal migration. Co-immunoprecipitation, in vitro kinase assay, dominant-negative mutants, neuronal migration assays Human molecular genetics High 16203747
2005 NudEL targets dynein to microtubule plus ends through LIS1; yeast Ndl1 (NudEL homolog) null mutants show decreased targeting of dynein to microtubule plus ends; Ndl1 interacts with LIS1 but not with CLIP170, and acts transiently at plus ends to promote LIS1 and dynein targeting. Yeast genetics (null mutant), live imaging of dynein/LIS1 at microtubule ends, co-immunoprecipitation Nature cell biology High 15965467
2007 The crystal structure of the coiled-coil domain of Ndel1 (residues 10–166) was determined at high resolution; the Ndel1 coiled coil forms a stable parallel homodimer, and the Lis1-interacting domain involves two supercoiled alpha helices that cooperatively bind to a Lis1 homodimer. X-ray crystallography, complementary solution studies (NMR/biophysics) Structure High 17997972
2008 Protein phosphatase 4 catalytic subunit (PP4c) dephosphorylates NDEL1 at Cdk1 sites; PP4c negatively regulates Cdk1 activity at the centrosome; loss of PP4c leads to abnormal NDEL1 phosphorylation and excessive katanin p60 recruitment, causing microtubule disorganization. In vitro phosphatase assay, Cdk1 activity assay, knockout mice, immunofluorescence, katanin p60 localization The Journal of cell biology High 18347064
2008 Nudel binds Cdc42GAP and competes with Cdc42 for Cdc42GAP binding; Nudel inhibits Cdc42GAP-mediated inactivation of Cdc42 in a dose-dependent manner; Nudel phosphorylation by Erk1/2 is required for its leading-edge localization; depleting Nudel abolishes Cdc42 activation and cell migration. Co-immunoprecipitation, in vitro competition binding assay, RNAi, Cdc42 activity assay, cell migration assay Developmental cell High 18331715
2005 NUDEL possesses oligopeptidase (cysteine protease) activity; mutation of Cys-273 fully abolishes this activity; DISC1 inhibits NUDEL-oligopeptidase activity in a competitive manner; the monomeric form is the active form in brain. Site-directed mutagenesis (C273A), in vitro peptidase assay, competitive inhibition assay with DISC1 Proceedings of the National Academy of Sciences of the United States of America High 15728732
2009 Nudel binds paxillin at nascent adhesions; this interaction is disrupted by focal adhesion kinase (FAK) in a paxillin-binding-dependent manner; forced localization of Nudel to all focal contacts strengthens adhesivity, and Nudel-paxillin interaction is important for cell edge protrusion and migration. Co-immunoprecipitation, RNAi, fusion protein targeting, cell migration/adhesion assays PLoS biology Medium 19492042
2009 Ndel1 and Nde1 undergo palmitoylation in vivo; unpalmitoylated Ndel1 interacts better with dynein; palmitoylated Ndel1 reduces cytoplasmic dynein activity as measured by Golgi distribution, VSVG trafficking, short microtubule transport, and neuronal migration. Palmitoylation assay (acyl-RAC), co-immunoprecipitation, dynein activity assays, neuronal migration assay The EMBO journal High 19927128
2005 Nudel is a mother centriole protein; its C-terminal region is required for associations with dynein, dynactin, PCM-1, pericentrin, and gamma-tubulin; Nudel participates in both dynein-mediated centripetal transport of dynactin/Lis1/PCM-1 and dynein-independent centrosomal targeting of pericentrin/gamma-tubulin; it tethers dynactin and dynein for microtubule anchoring. Immunofluorescence, RNAi, overexpression of deletion mutants, co-immunoprecipitation Molecular biology of the cell High 16291865
2004 NUDEL directly interacts with the neurofilament light subunit (NF-L) and facilitates NF polymerization; knockdown of NUDEL destabilizes NF-L and alters NF homeostasis, causing NF abnormalities and morphological changes reminiscent of neurodegeneration. RNAi, direct binding assay (co-immunoprecipitation), in vitro NF assembly assay, neuroblastoma cell line and primary cortical neurons Nature cell biology High 15208636
2010 Nudel contains a novel dynein binding domain within its first 80 amino acids that directly interacts with the dynein intermediate chain; both the dynein and Lis1 binding domains of Nudel are required for spindle pole organization; increased Lis1 rescues Nudel immunodepletion spindle defects, suggesting Nudel facilitates Lis1-dynein interaction. Xenopus egg extract spindle assembly assay, mutagenesis, immunodepletion, direct binding assay The Journal of biological chemistry High 21056974
2007 Nudel is required for robust localization of dynein/dynactin at kinetochores; Nudel localizes to kinetochores after NEB depending mostly on mitosin; Nudel activates dynein-mediated kinetochore protein transport and, when interacting with mitosin and dynein, stabilizes kinetochore dynein against microtubule-dependent stripping. RNAi, co-immunoprecipitation, immunofluorescence, kinetochore stripping assay Molecular biology of the cell High 17494871
2008 Lis1 and Ndel1 reduction impairs prophase nuclear envelope invagination (PNEI), a dynein-dependent process facilitating nuclear envelope breakdown (NEBD); Ndel1 phosphorylation regulates binding to both Lis1 and dynein and is important for this function; elevated Ndel1 phosphorylation at a key Cdk5 site is observed in prophase cells in the ventricular zone of Lis1+/- embryos. RNAi in mitotic cell line, phosphomimetic/phospho-dead mutants, live imaging, analysis of Lis1+/- embryo sections The Journal of cell biology High 18809722
2011 DISC1 and PDE4 modulate phosphorylation of NDE1 by PKA at Thr131; phosphorylation at T131 modulates NDE1-LIS1 and NDE1-NDEL1 interactions; T131-phosphorylated NDE1 is enriched at centrosome during mitosis; PKA phosphomimetic mutation of T131 inhibits neurite outgrowth. In vitro kinase assay, homology modeling, co-immunoprecipitation, neurite outgrowth assay, immunofluorescence The Journal of neuroscience High 21677187
2015 Elevated CDK5 activity (driven by p25) increases non-processive dynein events; CDK5 phosphorylation of Ndel1 favors a high-affinity Lis1/Ndel1/dynein complex that blocks ATP-dependent release of dynein from microtubules, inhibiting processive motility; transport disruption by CDK5 depends on the Lis1/Ndel1 complex. Live imaging of organelle transport in neurons, CDK5 inhibition/activation, in vitro dynein motility assay, co-immunoprecipitation Cell reports High 26166569
2013 LIS1 controls mitosis via the LIS1-NDEL1-dynein complex; overexpression of NDEL1-dynein and microtubule stabilization rescues spindle orientation defects in Lis1 mutant mouse embryonic fibroblasts, demonstrating that LIS1 acts through LIS1-NDEL1-dynein to regulate astral MT plus-end dynamics and cortical contact. Lis1 mutant MEFs, time-lapse live cell imaging, NDEL1 overexpression rescue, MT stabilization rescue Human molecular genetics High 24030547
2016 Ndel1 localizes to the subdistal appendage of the mother centriole and suppresses primary cilia formation by maintaining trichoplein levels via the trichoplein-Aurora A pathway; Ndel1 depletion reduces trichoplein at the mother centriole and induces unscheduled primary cilia formation, which is reverted by forced trichoplein expression or KCTD17 knockdown. siRNA knockdown, rescue experiments (trichoplein overexpression, KCTD17 co-knockdown), immunofluorescence, Ndel1-hypomorphic mice The Journal of cell biology High 26880200
2015 GSK-3β phosphorylates dynein intermediate chains at S87/T88 (IC-1B) and S88/T89 (IC-2C); this phosphorylation reduces IC interaction with Ndel1; inhibition of GSK-3β stimulates dynein motility in a manner dependent on Ndel1 binding to IC. Co-immunoprecipitation, in vitro kinase assay (mass spectrometry + site-directed mutagenesis), dynein motility assay, GSK-3β inhibitors/genetic manipulation Traffic High 26010407
2012 NDE1 and NDEL1 form dimers, tetramers, and chain-like polymers with a folded-back structure where the C-terminal domain interacts with the N-terminal coiled-coil; the C-terminal domain mediates interactions with dynein and DISC1; NDE1 and NDEL1 can directly interact with each other. Negative-stain electron microscopy, chemical cross-linking/mass spectrometry, isotope labeling The Journal of biological chemistry High 22843697
2008 Ndel1 directly binds PDE4 family members; the PDE4D3 isoform interaction is disrupted by PKA-mediated phosphorylation of Ser13 on PDE4D3 upon cAMP elevation; Ndel1 sequesters EPAC to form a cAMP signaling complex; PDE4 binding stabilizes Ndel1 self-interaction. Co-immunoprecipitation, scanning peptide array, BRET assay, PKA phosphorylation studies, in vitro binding Cellular signalling Medium 18845247
2008 Ndel1 forms a novel complex with vimentin, dynein, Lis1, and alphaCOP; Ndel1 promotes the interaction between Lis1, alphaCOP, and the vimentin-dynein complex to activate dynein-mediated transport of vimentin; loss of Ndel1 by RNAi reduces vimentin transport and causes IF accumulations. Tandem affinity purification, co-immunoprecipitation, RNAi, live imaging of vimentin transport The Journal of biological chemistry Medium 18303022
2009 Nudel is required for dynein-mediated axonal transport; microinjection of anti-Nudel antibody abolishes retrograde transport of membranous organelles in DRG axons; Nudel depletion causes lysosome accumulation in axons and delays endo-lysosome formation. Antibody microinjection into DRG neurons, live imaging of organelle transport Traffic Medium 19522757
2012 Nudel directly interacts with misfolded Gβ (both unubiquitinated and ubiquitinated forms) and recruits it to dynein for transport to the centrosome; depletion of Nudel reduces dynein-associated misfolded Gβ, impairs aggresome formation, and prolongs Gβ half-life. Co-immunoprecipitation, RNAi, aggresome formation assay, protein stability assay Cell research Medium 22430153
2012 Nudel promotes WAVE regulatory complex (WRC) assembly in vivo by stabilizing the Sra1-Nap1-Abi1 subcomplex through dynamic binding to Sra1 and protecting HSPC300 from proteasomal degradation; depletion of Nudel abolishes WRC-dependent actin polymerization and lamellipodial formation. Co-immunoprecipitation, RNAi, in vitro actin polymerization assay, cell spreading/lamellipodia imaging Cell research Medium 22453242
2011 Ndel1 directly associates with Dynamin 2 (Dyn2) and enhances Dyn2 GTPase activity in both unassembled and assembled forms in vitro; gain and loss of Ndel1 function phenocopies Dyn2 overexpression and dominant-negative Dyn2, respectively, on GluR1-containing membrane distribution. Co-immunoprecipitation, in vitro GTPase activity assay, overexpression and RNAi, immunofluorescence PLoS one Medium 21283621
2016 Ndel1 interacts with TRIO-associated repeat on actin (Tara); co-expression of Ndel1 and Tara causes synergistic increase in F-actin and filopodia formation; Tara redistributes Ndel1 to the cell periphery in a physical interaction-dependent manner, facilitating cell migration. Co-immunoprecipitation, RNAi, wound healing assay, Boyden chamber assay, immunofluorescence Scientific reports Medium 27546710
2019 Crystal structure of AnkB in complex with Ndel1 C-terminal coiled-coil (CT-CC) was determined at high resolution; Ndel1 CT-CC binds giant neuron-specific insertion regions of AnkG and AnkB with 2:1 stoichiometry via a 5-helix bundle; AnkG is essential for Ndel1 accumulation at the AIS; a peptide blocking the AnkG/Ndel1 interaction disrupts cargo sorting at the AIS. X-ray crystallography, co-immunoprecipitation, RNAi, cargo sorting assay, inhibitory peptide Proceedings of the National Academy of Sciences of the United States of America High 31889000
2017 NDEL1 is S-nitrosylated at Cys203 by neuronal nitric oxide synthase in an NMDA receptor-dependent manner; this modification accelerates dendritic arborization; disruption of S-nitrosylation of NDEL1 mediates impaired dendritic maturation caused by developmental alcohol exposure. Site-directed mutagenesis (C203 mutant), S-nitrosylation assay, NOS1 knockout mice, pharmacological inhibition of NMDA receptors, dendritic morphology quantification Cerebral cortex Medium 27371763
2013 LIS1, Ndel1, and NudCL have distinct functions in axonal mitochondrial transport; LIS1 interacts with KIF5b; depletion of LIS1 suppresses both anterograde and retrograde mitochondrial motility; depletion of both Ndel1 and NudCL together almost blocks retrograde mitochondrial transport. Co-immunoprecipitation (LIS1-KIF5b), siRNA knockdown, live imaging of mitochondrial transport in hippocampal neurons Traffic Medium 23551859
2015 DBZ (DISC1-binding zinc finger protein) regulates Ndel1 phosphorylation at Thr219 and Ser251; DBZ depletion or expression of double-phosphomimetic Ndel1 impairs anterograde transport of Lis1 and DISC1 to neurite tips and inhibits microtubule elongation. In vivo electroporation, phosphomimetic/non-phosphorylatable Ndel1 mutants, live imaging of Lis1/DISC1 transport, neurite extension assay The Journal of neuroscience Medium 25698733
2023 Ndel1 inhibits dynein activation in two ways: (1) it disfavors formation of the activated dynein-dynactin-adaptor complex, and (2) it sequesters LIS1 away from its dynein-binding site. Phosphomimetic mutations in Ndel1 C-terminal domain increase its inhibitory effect. The dynein intermediate chain N-terminus is an evolutionarily conserved hub interacting with dynactin and Ndel1; Ndel1 recruits LIS1 to dynein, and LIS1 must be handed off from Ndel1 to dynein in temporally discrete steps. Purified protein binding assays, single-molecule imaging, protein biochemistry, mutagenesis The Journal of biological chemistry / Nature communications High 37086789 37730751
2021 Ndel1 binds directly to keratin subunits through a motif conserved in all intermediate filament proteins; Ndel1 is required for robust desmosome-keratin association and for desmosomal localization (the latter requiring Lis1); loss of Ndel1 results in desmosome defects in mouse epidermis. Direct binding assay, mouse genetics, immunofluorescence, keratin reorganization assay Molecular biology of the cell Medium 34319758
2024 A de novo somatic mosaic NDEL1 variant p.R105P disrupts NDEL1 binding to LIS1; this variant strongly disrupts neuronal migration, increases leading process length, and impairs nucleus-centrosome coupling (nucleokinesis) when expressed alone; Ndel1 is expressed complementarily to Nde1 in post-mitotic neurons. In utero electroporation, single-cell RNA sequencing, spatial transcriptomics, co-immunoprecipitation (disrupted LIS1 binding), live imaging Acta neuropathologica High 38194050
2010 Oligopeptidase activity of Ndel1 is required for neurite outgrowth; the catalytically inactive mutant Ndel1(C273A) decreases neurite outgrowth and cannot rescue RNAi-mediated depletion, while wild-type Ndel1 rescues; Ndel1 expression and enzyme activity are upregulated during PC12 differentiation. Site-directed mutagenesis, RNAi rescue assay, PC12 neurite outgrowth assay Molecular and cellular neurosciences Medium 20462516
2009 Lis1 and Ndel1 reduction impairs prophase nuclear envelope invagination (PNEI); Ndel1 phosphorylation regulates its binding to both Lis1 and dynein for this function; Ndel1 phosphorylated at a Cdk5 site is elevated in prophase cells of the ventricular zone in Lis1+/- embryos. RNAi in cell line, phospho-mutant analysis, live imaging of NE dynamics, brain section immunostaining The Journal of cell biology Medium 18809722
2022 NDEL1 KD specifically impacts retrograde mitochondrial transport in adult axons in a LIS1-dependent manner; a LIS1 NDEL1-binding mutant (LIS1-R212A) dramatically impairs retrograde mitochondrial transport, revealing cargo-specific NDEL1 function distinct from its role in acidic organelle transport. siRNA KD, LIS1 mutant overexpression, live imaging of mitochondrial transport in adult DRG axons, sciatic nerve cross-section analysis Frontiers in molecular neuroscience Medium 35465088

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 NUDEL is a novel Cdk5 substrate that associates with LIS1 and cytoplasmic dynein. Neuron 412 11163260
2003 14-3-3epsilon is important for neuronal migration by binding to NUDEL: a molecular explanation for Miller-Dieker syndrome. Nature genetics 326 12796778
2002 Disrupted-in-Schizophrenia-1 (DISC-1): mutant truncation prevents binding to NudE-like (NUDEL) and inhibits neurite outgrowth. Proceedings of the National Academy of Sciences of the United States of America 325 12506198
2003 DISC1 (Disrupted-In-Schizophrenia 1) is a centrosome-associated protein that interacts with MAP1A, MIPT3, ATF4/5 and NUDEL: regulation and loss of interaction with mutation. Human molecular genetics 314 12812986
2004 Ndel1 operates in a common pathway with LIS1 and cytoplasmic dynein to regulate cortical neuronal positioning. Neuron 306 15473966
1998 The Saccharomyces cerevisiae NDE1 and NDE2 genes encode separate mitochondrial NADH dehydrogenases catalyzing the oxidation of cytosolic NADH. The Journal of biological chemistry 239 9733747
2010 Genetic mosaic dissection of Lis1 and Ndel1 in neuronal migration. Neuron 200 21092859
2007 DISC1 regulates the transport of the NUDEL/LIS1/14-3-3epsilon complex through kinesin-1. The Journal of neuroscience : the official journal of the Society for Neuroscience 197 17202468
2004 Disrupted in Schizophrenia 1 and Nudel form a neurodevelopmentally regulated protein complex: implications for schizophrenia and other major neurological disorders. Molecular and cellular neurosciences 187 14962739
2008 LIS1 and NDEL1 coordinate the plus-end-directed transport of cytoplasmic dynein. The EMBO journal 152 18784752
2006 DISC1-NDEL1/NUDEL protein interaction, an essential component for neurite outgrowth, is modulated by genetic variations of DISC1. Human molecular genetics 146 17035248
2007 Cenp-F links kinetochores to Ndel1/Nde1/Lis1/dynein microtubule motor complexes. Current biology : CB 138 17600710
2005 Complete loss of Ndel1 results in neuronal migration defects and early embryonic lethality. Molecular and cellular biology 133 16107726
2006 NDEL1 phosphorylation by Aurora-A kinase is essential for centrosomal maturation, separation, and TACC3 recruitment. Molecular and cellular biology 123 17060449
2003 Human Nudel and NudE as regulators of cytoplasmic dynein in poleward protein transport along the mitotic spindle. Molecular and cellular biology 118 12556484
2007 NudE and NudEL are required for mitotic progression and are involved in dynein recruitment to kinetochores. The Journal of cell biology 117 17682047
2004 Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein. The Journal of cell biology 108 14970193
2016 Dynein Regulator NDEL1 Controls Polarized Cargo Transport at the Axon Initial Segment. Neuron 107 26844830
2009 An essential role of the aPKC-Aurora A-NDEL1 pathway in neurite elongation by modulation of microtubule dynamics. Nature cell biology 105 19668197
2017 DISC1 Regulates Neurogenesis via Modulating Kinetochore Attachment of Ndel1/Nde1 during Mitosis. Neuron 102 29103808
2010 Functional interplay between LIS1, NDE1 and NDEL1 in dynein-dependent organelle positioning. Journal of cell science 102 20048338
2009 Requirement for Nudel and dynein for assembly of the lamin B spindle matrix. Nature cell biology 96 19198602
2008 Elucidating the relationship between DISC1, NDEL1 and NDE1 and the risk for schizophrenia: evidence of epistasis and competitive binding. Human molecular genetics 93 18469341
2011 A Cdk5-dependent switch regulates Lis1/Ndel1/dynein-driven organelle transport in adult axons. The Journal of neuroscience : the official journal of the Society for Neuroscience 92 22114287
1995 An unusual mosaic protein with a protease domain, encoded by the nudel gene, is involved in defining embryonic dorsoventral polarity in Drosophila. Cell 88 7671306
2005 NudEL targets dynein to microtubule ends through LIS1. Nature cell biology 87 15965467
2009 Distinct dose-dependent cortical neuronal migration and neurite extension defects in Lis1 and Ndel1 mutant mice. The Journal of neuroscience : the official journal of the Society for Neuroscience 85 20007476
2005 Recruitment of katanin p60 by phosphorylated NDEL1, an LIS1 interacting protein, is essential for mitotic cell division and neuronal migration. Human molecular genetics 85 16203747
2010 Evidence of statistical epistasis between DISC1, CIT and NDEL1 impacting risk for schizophrenia: biological validation with functional neuroimaging. Human genetics 81 20084519
2004 A NUDEL-dependent mechanism of neurofilament assembly regulates the integrity of CNS neurons. Nature cell biology 79 15208636
2005 Nudel contributes to microtubule anchoring at the mother centriole and is involved in both dynein-dependent and -independent centrosomal protein assembly. Molecular biology of the cell 77 16291865
2013 LIS1 controls mitosis and mitotic spindle organization via the LIS1-NDEL1-dynein complex. Human molecular genetics 76 24030547
2007 Nudel modulates kinetochore association and function of cytoplasmic dynein in M phase. Molecular biology of the cell 74 17494871
2008 Lis1 and Ndel1 influence the timing of nuclear envelope breakdown in neural stem cells. The Journal of cell biology 73 18809722
2011 PKA phosphorylation of NDE1 is DISC1/PDE4 dependent and modulates its interaction with LIS1 and NDEL1. The Journal of neuroscience : the official journal of the Society for Neuroscience 72 21677187
2007 The structure of the coiled-coil domain of Ndel1 and the basis of its interaction with Lis1, the causal protein of Miller-Dieker lissencephaly. Structure (London, England : 1993) 72 17997972
2008 Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation. The Journal of cell biology 68 18347064
2005 Inhibition of NUDEL (nuclear distribution element-like)-oligopeptidase activity by disrupted-in-schizophrenia 1. Proceedings of the National Academy of Sciences of the United States of America 65 15728732
2008 Nudel binds Cdc42GAP to modulate Cdc42 activity at the leading edge of migrating cells. Developmental cell 64 18331715
2016 Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway. The Journal of cell biology 62 26880200
2000 The nudel protease of Drosophila is required for eggshell biogenesis in addition to embryonic patterning. Developmental biology 55 10625559
2015 Stress-Induced CDK5 Activation Disrupts Axonal Transport via Lis1/Ndel1/Dynein. Cell reports 54 26166569
2005 Subcellular targeting of DISC1 is dependent on a domain independent from the Nudel binding site. Molecular and cellular neurosciences 52 15797709
2016 NDE1 and NDEL1 from genes to (mal)functions: parallel but distinct roles impacting on neurodevelopmental disorders and psychiatric illness. Cellular and molecular life sciences : CMLS 51 27742926
2010 Identification of a novel dynein binding domain in nudel essential for spindle pole organization in Xenopus egg extract. The Journal of biological chemistry 51 21056974
2015 GSK-3β Phosphorylation of Cytoplasmic Dynein Reduces Ndel1 Binding to Intermediate Chains and Alters Dynein Motility. Traffic (Copenhagen, Denmark) 47 26010407
1998 Role of Nudel protease activation in triggering dorsoventral polarization of the Drosophila embryo. Development (Cambridge, England) 43 9735365
2008 Ndel1 controls the dynein-mediated transport of vimentin during neurite outgrowth. The Journal of biological chemistry 42 18303022
2009 Nudel and FAK as antagonizing strength modulators of nascent adhesions through paxillin. PLoS biology 41 19492042
2009 Ndel1 palmitoylation: a new mean to regulate cytoplasmic dynein activity. The EMBO journal 41 19927128
2007 Expression patterns of LIS1, dynein and their interaction partners dynactin, NudE, NudEL and NudC in human gliomas suggest roles in invasion and proliferation. Acta neuropathologica 41 17221205
2001 NudE-L, a novel Lis1-interacting protein, belongs to a family of vertebrate coiled-coil proteins. Mechanisms of development 41 11231056
2013 Distinct functions of nuclear distribution proteins LIS1, Ndel1 and NudCL in regulating axonal mitochondrial transport. Traffic (Copenhagen, Denmark) 38 23551859
2013 NDE1 and NDEL1: twin neurodevelopmental proteins with similar 'nature' but different 'nurture'. Biomolecular concepts 37 24093049
2019 NFAT1-Mediated Regulation of NDEL1 Promotes Growth and Invasion of Glioma Stem-like Cells. Cancer research 36 30940662
2012 The mitosis and neurodevelopment proteins NDE1 and NDEL1 form dimers, tetramers, and polymers with a folded back structure in solution. The Journal of biological chemistry 36 22843697
2008 Ndel1 alters its conformation by sequestering cAMP-specific phosphodiesterase-4D3 (PDE4D3) in a manner that is dynamically regulated through Protein Kinase A (PKA). Cellular signalling 34 18845247
2010 The essential role of LIS1, NDEL1 and Aurora-A in polarity formation and microtubule organization during neurogensis. Cell adhesion & migration 33 20168084
2010 Assessing the role of endooligopeptidase activity of Ndel1 (nuclear-distribution gene E homolog like-1) in neurite outgrowth. Molecular and cellular neurosciences 33 20462516
1996 The maternal nudel protein of Drosophila has two distinct roles important for embryogenesis. Genetics 33 8844153
2009 Nudel promotes axonal lysosome clearance and endo-lysosome formation via dynein-mediated transport. Traffic (Copenhagen, Denmark) 32 19522757
2013 Plasma Ndel1 enzyme activity is reduced in patients with schizophrenia--a potential biomarker? Journal of psychiatric research 31 23388542
2011 Ndel1, Nudel (Noodle): flexible in the cell? Cytoskeleton (Hoboken, N.J.) 30 21948775
2022 miR-103-3p targets Ndel1 to regulate neural stem cell proliferation and differentiation. Neural regeneration research 25 34269216
2023 Conserved roles for the dynein intermediate chain and Ndel1 in assembly and activation of dynein. Nature communications 23 37730751
2009 Opposing effects of Ndel1 and alpha1 or alpha2 on cytoplasmic dynein through competitive binding to Lis1. Journal of cell science 22 19622634
2016 Regulation of the actin cytoskeleton by the Ndel1-Tara complex is critical for cell migration. Scientific reports 21 27546710
2015 Increased expression of NDEL1 and MBP genes in the peripheral blood of antipsychotic-naïve patients with first-episode psychosis. European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology 21 26476704
2015 DBZ regulates cortical cell positioning and neurite development by sustaining the anterograde transport of Lis1 and DISC1 through control of Ndel1 dual-phosphorylation. The Journal of neuroscience : the official journal of the Society for Neuroscience 20 25698733
2023 Ndel1 disfavors dynein-dynactin-adaptor complex formation in two distinct ways. The Journal of biological chemistry 19 37086789
2004 Expression of NUDEL in manchette and its implication in spermatogenesis. FEBS letters 18 15147871
2000 Biochemical defects of mutant nudel alleles causing early developmental arrest or dorsalization of the Drosophila embryo. Genetics 18 10628985
2022 Nde1 and Ndel1: Outstanding Mysteries in Dynein-Mediated Transport. Frontiers in cell and developmental biology 17 35493069
2020 Arsenic trioxide disturbs the LIS1/NDEL1/dynein microtubule dynamic complex by disrupting the CLIP170 zinc finger in head and neck cancer. Toxicology and applied pharmacology 17 32717241
2019 Mechanistic insights into the interactions of dynein regulator Ndel1 with neuronal ankyrins and implications in polarity maintenance. Proceedings of the National Academy of Sciences of the United States of America 16 31889000
2016 Ndel1 and Reelin Maintain Postnatal CA1 Hippocampus Integrity. The Journal of neuroscience : the official journal of the Society for Neuroscience 16 27307241
2014 The expression and roles of Nde1 and Ndel1 in the adult mammalian central nervous system. Neuroscience 16 24785679
2012 Nudel is crucial for the WAVE complex assembly in vivo by selectively promoting subcomplex stability and formation through direct interactions. Cell research 16 22453242
2019 Ndel1 oligopeptidase activity as a potential biomarker of early stages of schizophrenia. Schizophrenia research 14 30857875
2018 Oligopeptidases activity in bipolar disorder: Ndel1 and angiotensin I converting enzyme. Journal of affective disorders 13 30321766
2014 NDEL1 was decreased in the CA3 region but increased in the hippocampal blood vessel network during the spontaneous seizure period after pilocarpine-induced status epilepticus. Neuroscience 13 24680936
2012 Misfolded Gβ is recruited to cytoplasmic dynein by Nudel for efficient clearance. Cell research 13 22430153
2016 Genome-wide investigation of schizophrenia associated plasma Ndel1 enzyme activity. Schizophrenia research 12 26851141
2012 Ndel1-derived peptides modulate bidirectional transport of injected beads in the squid giant axon. Biology open 12 23213412
2021 Behavioral Deficits in Mice with Postnatal Disruption of Ndel1 in Forebrain Excitatory Neurons: Implications for Epilepsy and Neuropsychiatric Disorders. Cerebral cortex communications 11 33615226
2021 Expression and function of Ndel1 during the differentiation of neural stem cells induced by hippocampal exosomesticle. Stem cell research & therapy 10 33422130
2015 Protein-Protein and Peptide-Protein Interactions of NudE-Like 1 (Ndel1): A Protein Involved in Schizophrenia. Current protein & peptide science 10 25961396
2005 Cloning and characterization of the human and rabbit NUDEL-oligopeptidase promoters and their negative regulation. Biochimica et biophysica acta 10 16005531
2002 Evidence for a glycosaminoglycan on the nudel protein important for dorsoventral patterning of the drosophila embryo. Developmental dynamics : an official publication of the American Association of Anatomists 10 11984873
2024 Novel lissencephaly-associated NDEL1 variant reveals distinct roles of NDE1 and NDEL1 in nucleokinesis and human cortical malformations. Acta neuropathologica 9 38194050
2020 Role of NDEL1 and VEGF/VEGFR-2 in Mouse Hippocampus After Status Epilepticus. ASN neuro 9 32423231
2020 Reelin Improves Cognition and Extends the Lifespan of Mutant Ndel1 Mice with Postnatal CA1 Hippocampus Deterioration. Cerebral cortex (New York, N.Y. : 1991) 8 32328622
2018 Nudel involvement in the high-glucose-induced epithelial-mesenchymal transition of tubular epithelial cells. American journal of physiology. Renal physiology 8 30539652
2017 Developmental Alcohol Exposure Impairs Activity-Dependent S-Nitrosylation of NDEL1 for Neuronal Maturation. Cerebral cortex (New York, N.Y. : 1991) 8 27371763
2016 Status epilepticus stimulates NDEL1 expression via the CREB/CRE pathway in the adult mouse brain. Neuroscience 8 27298008
2023 Conserved Roles for the Dynein Intermediate Chain and Ndel1 in Assembly and Activation of Dynein. bioRxiv : the preprint server for biology 7 36711700
2022 LIS1 and NDEL1 Regulate Axonal Trafficking of Mitochondria in Mature Neurons. Frontiers in molecular neuroscience 7 35465088
2020 Evaluation of NDEL1 oligopeptidase activity in blood and brain in an animal model of schizophrenia: effects of psychostimulants and antipsychotics. Scientific reports 7 33116174
2021 Roles for Ndel1 in keratin organization and desmosome function. Molecular biology of the cell 6 34319758
2011 The cytoskeletal protein Ndel1 regulates dynamin 2 GTPase activity. PloS one 6 21283621