Affinage

NCK1

SH2/SH3 adapter protein NCK1 · UniProt P16333

Length
377 aa
Mass
42.9 kDa
Annotated
2026-06-10
100 papers in source corpus 44 papers cited in narrative 44 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NCK1 is a cytoplasmic adaptor protein that couples tyrosine-phosphorylated receptors and effectors to the actin polymerization machinery, thereby controlling actin dynamics, cell polarity, and directional migration (PMID:1333047, PMID:19917259, PMID:23444376). Through its SH2 domain it docks onto phosphotyrosine motifs in activated receptor tyrosine kinases and effectors, including PDGF and EGF receptors (PMID:1333047), Eph receptors (EphB1 pTyr594, EphA3 pTyr602) (PMID:9430661, PMID:19505147), the bacterial effector EPEC Tir pTyr474 (PMID:11918809, PMID:16636066), and Tks5 pTyr557 in invadopodia (PMID:19596797), while its three SH3 domains engage proline-rich effectors that drive actin assembly: WASP/N-WASP (PMID:7565724, PMID:17302440), PAK1 (PMID:8824201, PMID:8798379), and DCC (PMID:12149262). NCK1 is among the most potent activators of WASP/N-WASP-Arp2/3-mediated actin nucleation in vitro, operating at a defined 4 Nck:2 N-WASP:1 Arp2/3 stoichiometry and integrating phosphotyrosine and PI(4,5)P2 signals into localized actin polymerization (PMID:17302440, PMID:19917259, PMID:22613834). This scaffolding underlies diverse actin-dependent processes, including pathogen actin-based motility and entry (vaccinia virus, EPEC, picornaviruses) (PMID:11918809, PMID:12007418, PMID:31769754, PMID:24284073), FcγR-mediated phagocytosis (PMID:22454526), endothelial front-rear polarity and angiogenesis downstream of VEGF and Slit2/ROBO1 (PMID:23444376, PMID:26659946), and osteoblast migration toward IGF1 (PMID:26621720). In T cells, NCK1 binds the CD3ε ITAM/proline-rich region through a cooperative SH3.1/SH2 two-site engagement required for TCR signaling and T cell activation in vivo (PMID:18555270, PMID:26590318, PMID:24470497). NCK1 activity and abundance are tightly regulated: it is selectively ubiquitinated by c-Cbl at Lys178 (antagonized by synaptopodin) (PMID:24287595), translocated to the nucleus by SOCS7 where it is required for DNA-damage-induced p53 Ser15 phosphorylation and cell-cycle arrest (PMID:17803907), and it suppresses ER stress signaling by binding the PERK inhibitory site pTyr561 and modulating IRE1α/JNK activation, with consequences for insulin biosynthesis and glucose homeostasis (PMID:20587749, PMID:24371088). Beyond cytoskeletal scaffolding, NCK1 organizes the cytoplasmic capping complex by juxtaposing the capping enzyme and 5'-kinase on adjacent SH3 domains and promotes both cap-dependent and -independent translation via eIF2β binding (PMID:11959995, PMID:25137142).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1992 High

    Established NCK1 as a phosphotyrosine-binding adaptor coupled to growth factor receptors and capable of cellular transformation, defining its core scaffolding identity.

    Evidence Co-IP and GST pulldown with activated PDGF/EGF receptors plus NIH 3T3/3Y1 focus and soft-agar transformation assays

    PMID:1280326 PMID:1333047

    Open questions at the time
    • Did not identify the SH3-bound effectors mediating transformation
    • Mechanism linking receptor binding to oncogenic output unresolved
  2. 1995 High

    Identified WASP as the first SH3-domain effector of NCK1, linking the adaptor to the actin nucleation machinery.

    Evidence Yeast two-hybrid screen with in vitro/in vivo binding and subcellular fractionation

    PMID:7565724

    Open questions at the time
    • Did not demonstrate functional actin polymerization output
    • Receptor-to-WASP coupling not yet reconstituted
  3. 1996 High

    Mapped the NCK1 second SH3 domain to PAK1 and showed this links receptor tyrosine kinases to PAK1 kinase activation, defining a receptor-to-kinase relay.

    Evidence Domain-mapped Co-IP and kinase activity assays in COS-7, HeLa, and Swiss 3T3 cells with PDGFR stimulation

    PMID:8798379 PMID:8824201

    Open questions at the time
    • Downstream cytoskeletal consequences of PAK1 activation not measured
    • Did not establish in vivo pathway context
  4. 1998 High

    Placed NCK1 in Eph receptor and TCR signaling pathways, showing it relays distinct downstream outputs (JNK vs NFAT) depending on context.

    Evidence Yeast two-hybrid, Y594F mutagenesis, dominant-negative overexpression, NFAT/JNK reporter and kinase assays in T cells

    PMID:9430661 PMID:9755165

    Open questions at the time
    • Effector specificity determinants for JNK vs NFAT not defined
    • Did not resolve direct vs indirect contributions
  5. 2000 High

    Defined regulatory logic of the NCK1-PAK1 interaction (phospho-dependent motif recognition and autophosphorylation-controlled release) and the NIK/Nck integrin-activation module.

    Evidence Saturation peptide substitution binding analysis, phosphorylation assays, and Co-IP/kinase/integrin assays downstream of EphB receptors

    PMID:10669731 PMID:10805734

    Open questions at the time
    • In vivo physiological role of phospho-regulated cycling not tested
    • NIK identity and broader role not fully characterized
  6. 2002 High

    Demonstrated NCK1 is hijacked by pathogens for actin-based motility and revealed non-cytoskeletal roles in translation and netrin/DCC signaling.

    Evidence EPEC Tir pTyr474 binding/localization, vaccinia A36R phosphomutant actin tail assays, DCC Co-IP/Rac1/neurite assays, and eIF2β binding with in vitro translation assays

    PMID:11918809 PMID:11959995 PMID:12007418 PMID:12149262

    Open questions at the time
    • Translation role mechanism beyond eIF2β binding not resolved
    • DCC findings from single lab with Medium confidence
  7. 2004 Medium

    Identified an ER-associated NCK1 pool that restrains ERK-1 and stress signaling, expanding NCK1 into ER-stress regulation.

    Evidence ER membrane IP, in vitro reconstitution, and kinase assays in Nck-null fibroblasts

    PMID:15201339

    Open questions at the time
    • Molecular ER tether not identified
    • Single-lab finding without reciprocal validation
  8. 2006 High

    Provided atomic-resolution basis for NCK1 SH2 phosphopeptide recognition and confirmed indistinguishable Nck1/Nck2 SH2 specificity, anchoring binding-mode interpretation.

    Evidence X-ray crystallography of SH2-Tir phosphopeptide complexes with mutagenesis and pulldowns

    PMID:16636066

    Open questions at the time
    • Does not explain paralog-specific functional divergence given identical SH2 specificity
    • SH3 selectivity not structurally addressed
  9. 2007 High

    Established NCK1 as a top-tier in vitro activator of WASP/N-WASP and revealed an unexpected SOCS7-driven nuclear function in the DNA-damage response.

    Evidence In vitro actin nucleation with purified proteins and nuclear/cytoplasmic rescue, ATM/ATR epistasis, and p53 Ser15 phosphorylation assays

    PMID:17302440 PMID:17803907

    Open questions at the time
    • Nuclear NCK1 effectors mediating cell-cycle arrest not identified
    • How nuclear vs cytoplasmic pools are balanced under stress unresolved
  10. 2009 High

    Showed NCK1 integrates phosphotyrosine and PI(4,5)P2 inputs to drive localized actin polymerization, and extended this to invadopodia matrix degradation.

    Evidence Nck KO/KD with PI(4,5)P2 manipulation and live imaging of actin comets; Tks5 Y557 mutagenesis and gelatin degradation assays

    PMID:19242519 PMID:19505147 PMID:19596797 PMID:19917259

    Open questions at the time
    • Spatial coincidence detection mechanism of dual signals not fully defined
    • Paralog non-redundancy mechanism for GTPase selectivity unclear
  11. 2012 High

    Quantified the stoichiometric and density requirements for NCK1-driven actin assembly, converting the scaffold model into a quantitative threshold model.

    Evidence Computational modeling with antibody-induced membrane aggregation and quantitative live-cell fluorescence; FcγR phagocytosis dependency

    PMID:22454526 PMID:22613834

    Open questions at the time
    • In vivo relevance of the 4:2:1 stoichiometry at physiological receptors untested
    • FcγR study single-lab Medium confidence
  12. 2013 High

    Revealed paralog-specific regulation (c-Cbl ubiquitination of NCK1 Lys178), defined NCK1 control of front-back polarity, and discovered the PERK-binding metabolic axis.

    Evidence K178R/ubiquitination and synaptopodin competition assays; FRET/AFM polarity imaging in endothelial cells; PERK Y561F mutagenesis with KO mice and β-cell insulin readouts

    PMID:23444376 PMID:24287595 PMID:24371088

    Open questions at the time
    • How c-Cbl regulation intersects with signaling outputs in vivo not resolved
    • Mechanism by which NCK1 controls RhoA versus Cdc42/Rac balance incomplete
  13. 2014 High

    Defined the cooperative two-site NCK1-CD3ε engagement required for TCR signaling in vivo and established NCK1 as the scaffold for the cytoplasmic capping complex.

    Evidence Proximity ligation, KI-PRS knock-in mice, EAE and peptide-inhibitor studies; in vitro capping-complex reconstitution on recombinant Nck1 with SH3 mutagenesis

    PMID:24470497 PMID:25137142 PMID:26590318

    Open questions at the time
    • Regulation of capping complex assembly under physiological signaling unclear
    • Whether mRNA recapping targets are sequence-selective not addressed
  14. 2015 High

    Established NCK1 (with NCK2) as essential for VEGF/Slit2-ROBO1-driven endothelial polarity and angiogenesis and for IGF1-directed osteoblast migration and bone formation.

    Evidence Endothelial- and osteoblast-specific conditional Nck1/2 KO mice with Cdc42/PAK activation, ROBO1/IRS-1 Co-IP, and in vivo vascular/bone phenotyping

    PMID:26621720 PMID:26659946

    Open questions at the time
    • Individual NCK1 versus NCK2 contributions in these tissues not separated
    • Receptor-proximal coupling to ROBO1 not structurally defined
  15. 2018 High

    Demonstrated NCK1/2 act as negative regulators of PDGFRβ signaling in pericytes and resolved NCK1- versus NCK2-specific interactomes and functions (NCK2 in cytokinesis).

    Evidence Mural-cell conditional KO with PDGFRβ Y1009 phosphorylation and retinopathy models; AP-MS/BioID interactomes and Nck-KO MEF cytokinesis imaging

    PMID:30002203 PMID:30150707

    Open questions at the time
    • Molecular basis of NCK1 acting as PDGFR brake versus relay context-dependence unclear
    • Cytokinesis role is NCK2-specific, leaving NCK1's mitotic role undefined
  16. 2020 High

    Identified an NCK1-selective inflammatory pathway in atherosclerosis, linking disturbed flow to NF-κB via an NCK1-IRAK1 interaction.

    Evidence Nck1 vs Nck2 KO mice, domain mutagenesis, Nck1-IRAK1 Co-IP, NF-κB reporters, flow chambers, and high-fat-diet atherosclerosis with bone marrow chimeras

    PMID:32427580

    Open questions at the time
    • Structural basis of NCK1-IRAK1 selectivity over NCK2 not defined
    • How a cytoskeletal adaptor engages an innate-immune kinase mechanistically unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unknown how the molecular features distinguishing NCK1 from NCK2 — given indistinguishable SH2 specificity — generate paralog-specific functions across actin, inflammatory, metabolic, and nuclear pathways.
  • No structural determinant explaining NCK1-selective partners (IRAK1, c-Cbl substrate, PERK) identified
  • Integration logic that routes NCK1 between cytoskeletal scaffolding and translation/capping roles undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 7 GO:0008092 cytoskeletal protein binding 4 GO:0098772 molecular function regulator activity 4
Localization
GO:0005829 cytosol 3 GO:0005886 plasma membrane 3 GO:0005783 endoplasmic reticulum 2 GO:0005634 nucleus 1 GO:0005840 ribosome 1
Pathway
R-HSA-162582 Signal Transduction 5 R-HSA-168256 Immune System 5 R-HSA-1266738 Developmental Biology 4 R-HSA-1643685 Disease 4 R-HSA-8953897 Cellular responses to stimuli 4 R-HSA-8953854 Metabolism of RNA 2
Partners
CBLIRAK1PAK1PERK/EIF2AK3ROBO1TKS5/SH3PXD2AWASWASL
Complex memberships
N-WASP/Arp2/3 actin nucleation complexcytoplasmic capping complex

Evidence

Reading pass · 44 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1992 NCK1 (Nck) SH2 domain mediates association with tyrosine-autophosphorylated PDGF and EGF receptors in response to ligand activation; Nck is phosphorylated on tyrosine, serine, and threonine residues upon growth factor stimulation; ~5-fold overexpression of Nck in NIH 3T3 cells induces oncogenic foci formation. Co-immunoprecipitation with anti-Nck antibodies, in vitro binding with GST-Nck fusion protein, NIH 3T3 focus formation assay Molecular and cellular biology High 1333047
1992 Overexpression of NCK1 in 3Y1 rat fibroblasts causes transformation (altered morphology, soft agar colony formation, tumor formation in nude mice) without detectable elevation of phosphotyrosine levels; GST-Nck binds p60v-src and serine/threonine kinases from cell lysates. Soft agar colony formation, tumor formation in nude mice, GST fusion protein pulldown, Western blot Molecular and cellular biology High 1280326
1995 The C-terminal (third) SH3 domain of NCK1 (p47nck) specifically interacts with WASP (Wiskott-Aldrich syndrome protein) in vivo and in vitro; WASP is mainly cytosolic. Yeast two-hybrid/cDNA expression library screen, in vitro and in vivo binding assays, subcellular fractionation, Western blot Molecular and cellular biology High 7565724
1996 NCK1 binds PAK1 through its second SH3 domain; PAK1 interacts with Nck via its first N-terminal proline-rich SH3-binding motif; the Nck–PAK1 interaction is strengthened upon PDGF receptor stimulation; active PAK1 association with Nck leads to phosphorylation of Nck at multiple sites. Co-immunoprecipitation in COS-7 and Swiss 3T3 cells, in vitro binding, domain-mapping with individual SH3 domain constructs The Journal of biological chemistry High 8824201
1996 NCK1 SH2 domain recruits Nck to activated EGF and PDGF receptors; Nck second SH3 domain binds PAK1 proline-rich N-terminus; Nck–PAK1 interaction links receptor tyrosine kinases to PAK1 and enhances PAK1 kinase activity when Nck is co-transfected. Co-immunoprecipitation in living cells, domain mapping, kinase activity assay, co-transfection in HeLa cells The Journal of biological chemistry High 8798379
1998 NCK1 SH2 domain binds phosphorylated juxtamembrane Tyr594 of EphB1 receptor; Nck recruitment to EphB1 is required for downstream JNK/SAPK activation and cell attachment responses. Yeast two-hybrid screen, co-immunoprecipitation of native receptor complexes, site-directed mutagenesis (Y594F), JNK activity assay The Journal of biological chemistry High 9430661
1998 NCK1 and PAK1 form a signaling module downstream of TCR activation; this module is required specifically for NFAT activation but not for JNK activation; Pak1 acts downstream of Vav and Cdc42; dominant-negative Nck or Pak1 inhibits TCR-mediated NFAT and Erk2 activation. Co-immunoprecipitation, dominant-negative overexpression, NFAT luciferase reporter assay, kinase activity assay in T cells The EMBO journal High 9755165
2000 NCK1 second SH3 domain binds an 18-mer PAK-derived peptide containing the motif PXXPXRXXS; serine phosphorylation within this motif negatively regulates NCK1-PAK1 binding; PAK autophosphorylation blocks binding of both Nck and PIX to PAK1, providing a mechanism to regulate PAK interactions and its cycling between cytosolic and focal complex sites. Saturation substitution binding analysis, in vitro binding assays with peptides and full-length proteins, phosphorylation assays Molecular and cellular biology High 10805734
2000 Nck-interacting kinase (NIK) is activated by EphB1 and EphB2 receptors in a manner requiring EphB1 kinase activity and phosphorylation of juxtamembrane Tyr594; NIK activation through NCK1 is required for EphB1-mediated integrin activation and JNK activation; a complex of NIK/Nck, p62(dok), and RasGAP forms upon EphB receptor stimulation. Co-immunoprecipitation, kinase activity assays, dominant-negative experiments, integrin activation assay Molecular and cellular biology High 10669731
2002 A 12-amino-acid sequence of EPEC Tir containing phospho-Tyr474 binds the host adaptor NCK1; NCK1 is recruited to actin pedestals of EPEC in a Y474 phosphorylation-dependent manner and is required for EPEC-induced actin signaling. Chimeric protein expression, in vivo binding assay, immunofluorescence localization of endogenous Nck to pedestals Molecular microbiology High 11918809
2002 NCK1 SH3 domains interact directly with DCC (deleted in colorectal cancer) receptor, independent of the SH2 domain; this interaction mediates DCC/Nck-1 association with the actin cytoskeleton and is required for netrin-1-induced Rac1 activation and neurite outgrowth. Co-immunoprecipitation in commissural neurons, dominant-negative Nck-1 overexpression, Rac1 activation assay, neurite outgrowth assay The Journal of biological chemistry Medium 12149262
2002 NCK1 SH3 domains interact with eIF2β; Nck is present in ribosomal fractions and its ribosomal localization is enhanced by insulin; overexpression of Nck-1 enhances both cap-dependent and -independent protein translation in a manner requiring the first and third SH3 domains; Nck-1 directly increases protein translation in vitro. Co-immunoprecipitation, cell fractionation/ribosomal enrichment, in vitro translation assay, domain mutants Proceedings of the National Academy of Sciences of the United States of America Medium 11959995
2002 Grb2 and NCK1 act cooperatively to promote actin-based motility of vaccinia virus; Nck is recruited to viral particles via phospho-Tyr112 of A36R, whereas Grb2 is recruited via phospho-Tyr132; the Grb2 pathway requires prior Nck-mediated N-WASP recruitment. Immunofluorescence, phosphomutant constructs, actin tail counting, co-immunoprecipitation Current biology : CB High 12007418
2004 ER-associated NCK1 represses ERK-1 activation under basal conditions; upon ER stress, this pool of Nck dissociates from ER membranes, allowing ERK-1 activation; Nck-null cells show stronger ERK-1 activation after ER stress correlating with enhanced cell survival. Immunoprecipitation of ER membranes, in vitro reconstitution with ER membranes, Nck-null fibroblasts, kinase assays Molecular biology of the cell Medium 15201339
2006 Crystal structures of Nck1 and Nck2 SH2 domains in complex with phosphopeptides from EPEC Tir establish highly conserved phosphopeptide recognition; Nck1 and Nck2 SH2 domains have essentially indistinguishable binding specificities; GIT1 is confirmed experimentally as a new Nck binding partner. X-ray crystallography, phosphopeptide binding studies, sequential mutagenesis, pulldown assays The Journal of biological chemistry High 16636066
2007 NCK1 nuclear accumulation (carried by SOCS7 via nuclear import/export signals) causes actin stress fiber disintegration and cell polarity loss; nuclear NCK1 is essential for cell-cycle arrest after DNA damage; the septin–SOCS7–NCK axis functions downstream of ATM/ATR and is required for p53 Ser15 phosphorylation. RNAi knockdown, cytoplasmic vs. nuclear NCK1 rescue constructs, live imaging, DNA damage assays, epistasis with ATM/ATR inhibitors, p53 phosphorylation immunoblot Cell High 17803907
2007 NCK1 (and NCK2) are the most potent activators of WASP and N-WASP in vitro; Nck1/2 differentially regulate WASP vs. N-WASP (Nck is a more potent activator than Cdc42 or Rac1); in vitro actin nucleation assays with purified full-length proteins. In vitro actin nucleation assay with purified full-length WASP/N-WASP, Arp2/3 complex, and various activators Biochemistry High 17302440
2008 NCK1 SH3.1 domain forms a complex with an atypical PxxDY motif at Tyr166 of CD3ε (non-ITAM-requiring interaction); Nck binding inhibits phosphorylation of the CD3ε ITAM by Fyn and Lck in vitro; Nck–CD3ε interaction downregulates TCR surface expression upon physiological stimulation. NMR structure of complex, in vitro kinase assay, flow cytometry for TCR surface expression in primary mouse lymphocytes Journal of molecular biology High 18555270
2009 Src phosphorylates Tks5 at Y557, inducing direct association with NCK1 (and NCK2) SH2 domains in invadopodia; Tks5 mutants unable to bind Nck show reduced matrix degradation and inefficient actin recruitment; Nck1 depletion inhibits Src–Tks5-driven matrix proteolysis and actin assembly in invadopodia. Co-immunoprecipitation, site-directed mutagenesis of Y557, Nck1 siRNA knockdown, gelatin matrix degradation assay, immunofluorescence Journal of cell science High 19596797
2009 NCK1 SH2 domain binds phospho-Tyr602 of EphA3 receptor; this interaction mediates EphA3-induced inhibition of cell migration and process retraction; dominant-negative Nck1 mutants lacking SH2 or SH3 domains partially alleviate EphA3-mediated inhibitory effects. Yeast two-hybrid, GST pulldown, co-immunoprecipitation, SH2 domain mutagenesis, cell migration assay Biochemistry Medium 19505147
2009 NCK1 is essential for actin remodeling stimulated by PI(4,5)P2, and PI(4,5)P2 is necessary for NCK1-induced localized actin polymerization in vivo; Nck knockdown/knockout suppresses PI(4,5)P2-induced actin comets; Nck couples phosphotyrosine- and phosphoinositide-dependent signals to N-WASp/Arp2/3 actin polymerization. Nck siRNA knockdown, Nck-knockout cells, antibody-induced Nck aggregation, live-cell imaging of actin comets, PI(4,5)P2 manipulation with inositol 5-phosphatase Molecular cell High 19917259
2010 Nck1 specifically localizes to invadopodia of metastatic mammary carcinoma cells but not to podosomes in macrophages or Src-transformed fibroblasts, establishing distinct upstream activators for invadopodia vs. podosomes. Confocal immunofluorescence microscopy of endogenous Nck1 in multiple cell types European journal of cell biology Medium 20850195
2010 Nck1 deletion in obese mice attenuates hepatic IRE1α and JNK activation and IRS-1 phosphorylation at Ser307, improving glucose disposal and insulin signaling; siRNA knockdown of Nck1 in HepG2 cells decreases thapsigargin-induced IRE1α activation. Nck1−/− obese mice (in vivo), siRNA knockdown in HepG2 cells, kinase activation assays, glucose tolerance tests, insulin signaling (Akt phosphorylation) American journal of physiology. Endocrinology and metabolism High 20587749
2012 The stoichiometry of NCK1-dependent actin polymerization through the N-WASp/Arp2/3 pathway is 4 Nck : 2 N-WASp : 1 Arp2/3; the density of Nck molecules in membrane aggregates is a critical determinant of localized actin polymerization. Computational modeling (Virtual Cell), antibody-induced aggregation of membrane-targeted Nck SH3 domains, quantitative fluorescence microscopy in living cells The Journal of cell biology High 22613834
2013 NCK1 (and Nck2) is essential for front-back polarity, polarized membrane protrusion, and directional migration of endothelial cells; Nck depletion causes delocalized Cdc42/Rac activity and reduces RhoA activity and myosin II phosphorylation; Nck couples polarized protrusion formation with adhesion maturation via integrin α5β1-fibronectin interaction. siRNA knockdown, time-lapse DIC and TIRF microscopy, FRET imaging for Cdc42/Rac/RhoA activity, atomic force microscopy for adhesion force Journal of cell science High 23444376
2013 NCK1 (but not Nck2) is a substrate of c-Cbl-mediated ubiquitination at the evolutionarily conserved Lys178; synaptopodin competes with c-Cbl for Nck1 binding, preventing Nck1 ubiquitination; c-Cbl knockdown restores Nck1 protein levels and stress fibers; NCK1-specific SH3 domain 2 drives RhoA activation and actin stress fiber formation. Ubiquitination assays, site-directed mutagenesis (K178R), co-immunoprecipitation, gene silencing (c-Cbl siRNA), RhoA activation assay, immunofluorescence for stress fibers Nature communications High 24287595
2013 NCK1 SH2 domain binds phospho-Y561 in the PERK juxtamembrane domain; this interaction negatively regulates PERK activity (pY561PERK is an inhibitory site); Nck1 silencing in MIN6 cells accelerates pY561PERK dephosphorylation, enhances PERK activation, increases insulin biosynthesis and proinsulin content; Nck1-knockout mice pancreatic islets contain more insulin. Co-immunoprecipitation, site-directed mutagenesis (Y561F), siRNA in MIN6 cells, Nck1-KO mice, PERK phosphorylation kinetics, insulin/proinsulin content assays Molecular biology of the cell High 24371088
2014 NCK1 is required for assembly of the cytoplasmic capping complex; capping enzyme (CE) binds Nck1 via its proline-rich C-terminus; CE and the 5'-kinase are juxtaposed by binding adjacent SH3 domains of Nck1; Nck1 knockdown disrupts complex integrity; cap homeostasis is inhibited by Nck1 with inactivating mutations in the CE- or kinase-binding SH3 domains. Co-immunoprecipitation, sedimentation, gel filtration, in vitro complex assembly on recombinant Nck1, siRNA knockdown, SH3-domain mutagenesis PLoS biology High 25137142
2014 NCK1 SH3.1 domain binds the proline-rich sequence (PRS) of CD3ε when the first ITAM tyrosine is unphosphorylated; the SH2 domain simultaneously binds the second ITAM tyrosine when phosphorylated; cooperative two-site engagement is required for Nck1 binding to the TCR; SH3.1-mutated Nck1 impairs TCR signaling and T cell activation. In situ proximity ligation assay, co-purification, domain mutations, TCR signaling readouts in Jurkat T cells Journal of immunology High 26590318
2014 NCK1 SH3.1 domain binding to the CD3ε PRS is required for T cell activation in vivo; KI-PRS mice with a mutation preventing Nck–CD3ε interaction show impaired CD3ζ phosphorylation, reduced T cell activation by antigen in vitro, partial protection from EAE, and deficient antitumoral response; a high-affinity SH3.1-blocking peptide inhibits T cell proliferation in vitro and in vivo. Knock-in mouse model, TCR signaling assays (CD3ζ phosphorylation), EAE model, peptide inhibitor in vivo Journal of immunology High 24470497
2015 NCK1 and NCK2 inactivation in endothelial cells inhibits Cdc42 and PAK2 activation by VEGF-A and Slit2, preventing front-rear polarity; NCK1/2 binding to ROBO1 is required for both Slit2- and VEGF-induced front-rear polarity; endothelial-specific Nck1/2 deletion causes severe angiogenesis defects in postnatal retina and embryonic development. Inducible endothelial-specific Nck1/2 knockout mice, Cdc42/PAK2 activation assays, co-immunoprecipitation of NCK with ROBO1, retinal and embryonic vascular phenotyping Circulation High 26659946
2015 NCK1 (and NCK2) in osteoblasts regulates preosteoblast/osteoblast migration toward IGF1; Nck1 binds IRS-1 (by co-immunoprecipitation); conditional double deletion of Nck1/2 in osteoblasts causes osteopenia, suppresses bone formation rate in vivo, and impairs bone injury repair, without affecting bone resorption. shRNA knockdown, Nck1 overexpression, co-immunoprecipitation (Nck1/IRS-1), osteoblast-specific conditional KO mice, in vivo bone histomorphometry, calvarial defect model Proceedings of the National Academy of Sciences of the United States of America High 26621720
2018 NCK1 and NCK2 adaptors in pericytes inhibit PDGF-B-induced phosphorylation of PDGFRβ at Y1009 and PAK activation; inactivation of NCK1/2 in mural cells prevents pathological neovascular tuft formation and vascular leakage in oxygen-induced retinopathy model. Inducible mural cell-specific Nck1/2 knockout mice, PDGFRβ phosphorylation assay, PAK activation assay, oxygen-induced retinopathy model, lineage tracing Nature communications High 30150707
2018 Proteomic analysis identifies 30 NCK1-specific and 28 NCK2-specific interactors; NCK2 (not NCK1) is required for cell abscission during cytokinesis—Nck2-deficient MEFs are multinucleated, show extended intercellular bridges, fail to complete abscission, and have altered midbody composition (mislocalized AURKB, PLK1, ECT2); NCK2 function in cytokinesis requires its SH2 domain. Affinity purification-MS, BioID proximity labeling, Nck1/Nck2-KO MEFs, live-cell time-lapse imaging of cytokinesis, immunofluorescence of midbody markers, SH2 domain mutants Molecular & cellular proteomics : MCP High 30002203
2020 Only NCK1 (not NCK2) mediates disturbed flow-induced NF-κB activation in endothelial cells; NCK1 SH2 domain and first SH3 domain are required for this effect; IRAK-1 is identified as an NCK1-selective binding partner; disturbed flow-induced IRAK-1 activation requires NCK1 in vitro and in vivo; Nck1-knockout mice show reduced endothelial activation and atherosclerotic plaque formation. Nck1-KO and Nck2-KO mice, domain-swap and point-mutation experiments, co-immunoprecipitation (Nck1–IRAK1), NF-κB reporter assays, flow chamber experiments, high fat diet atherosclerosis model, bone marrow chimeras The Journal of clinical investigation High 32427580
2019 NCK1 functions in a common pathway with TNK2 and WASL for picornavirus (EMCV, CVB3, poliovirus, EV-D68) entry/infection; CRISPR deletion of NCK1 reduces infection by multiple picornaviruses; genetic epistasis places NCK1, TNK2, and WASL in the same pathway for viral internalization. CRISPR deletion, viral infection assays, genetic epistasis analysis, EMCV internalization assay, mouse model (TNK2 KO) eLife High 31769754
2011 NCK1 (Dock/Nck) forms a stable complex with PTP1B (PTP61F in Drosophila); this Nck/PTP1B complex inducibly associates with the insulin receptor; Nck is required for effective insulin receptor dephosphorylation and inactivation by PTP1B in mammalian cells. Co-immunoprecipitation, Drosophila genetic assays (PTP61F/Dock interaction), IR dephosphorylation assay in vitro and in vivo The Biochemical journal Medium 21707536
2011 PAK1-Nck complex formation sequesters PAK1 in the cytoplasm; co-expression of PAK1 and NCK1 inhibits PRL-induced cyclin D1 promoter activity by 95%; disruption of PAK1-Nck binding partially abolishes this inhibition. Co-immunoprecipitation, cytoplasmic/nuclear fractionation, cyclin D1 luciferase reporter assay, dominant-negative and binding-deficient mutants Molecular endocrinology Medium 21719533
1999 The third (C-terminal) SH3 domain of NCK1 specifically binds Sos; the third SH3 domain also binds dynamin (a GTP-binding protein involved in clathrin-coated vesicle formation); Nck and dynamin co-precipitate from cell lysates. GST fusion protein pulldown with individual SH3 domains, co-immunoprecipitation from K562 cells, protein identification by molecular mass Cellular signalling Medium 10206341
1999 Tyrosine 351 of DokR (Dok-related protein) mediates binding to NCK1 SH2 domain; this interaction is dependent on DokR tyrosine phosphorylation by Lyn kinase; DokR–Nck complex forms upon EGF receptor activation. GST fusion protein binding assay, site-directed mutagenesis of DokR tyrosines, co-immunoprecipitation in 293T cells and BaF/3 cells The Journal of biological chemistry Medium 10428862
2006 cGMP-dependent protein kinase (PKG) phosphorylates PAK1 at Ser21, causing release of NCK1 from PAK1 and promoting formation of a new PAK1-VASP complex; Ser21 phosphorylation of PAK1 regulates cell polarization and tail retraction, and cells expressing S21A-PAK1 show aberrant morphology when NCK/PAK binding is blocked. In vitro phosphorylation mapping, phosphospecific antibody, co-transfection assays, endothelial cell morphology assays, peptide-mediated disruption of Nck/PAK binding The Journal of biological chemistry Medium 16490784
2013 NCK1 interacts with Cdc42 and the Rho-GEF intersectin-1 (ITSN1) to promote N-WASP-dependent actin polymerization during vaccinia virus actin tail formation and FcγR-mediated phagocytosis; ITSN1 is recruited prior to actin-based motility and functions with Cdc42 in a feed-forward loop with N-WASP and Nck. Immunofluorescence, siRNA knockdown, co-immunoprecipitation, phagocytosis assay, vaccinia actin tail assay Journal of cell science Medium 24284073
2012 NCK1 is required for FcγR- but not CR3-mediated phagocytosis; Nck is recruited to phagocytic cups via phospho-Tyr282 and Tyr298 in the FcγR ITAM; Nck promotes phagocytosis by recruiting N-WASP and requires Cdc42 interaction with the N-WASP CRIB domain for efficient phagocytosis. Nck siRNA knockdown, FcγR phosphomutants (Y282F/Y298F), immunofluorescence of N-WASP/Cdc42 localization, phagocytosis assays Journal of cell science Medium 22454526
2009 NCK1 (and Nck2) plays a non-compensating role in PDGF-BB-stimulated dermal fibroblast migration; Nckalpha mediates Cdc42 signaling for filopodium formation, while Nckbeta mediates Rho signaling for stress fiber induction; PDGFRβ-Y751F (preventing Nckalpha binding) and Y1009F (preventing Nckbeta binding) each abolish PDGF-BB-induced migration. PDGFRβ phosphosite mutants, Nck-KO MEFs, human dermal fibroblasts with siRNA, GTPase activation assays, cell migration assays The Journal of investigative dermatology High 19242519

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1995 Wiskott-Aldrich syndrome protein physically associates with Nck through Src homology 3 domains. Molecular and cellular biology 279 7565724
1996 Interaction of the Nck adapter protein with p21-activated kinase (PAK1). The Journal of biological chemistry 274 8824201
1996 The adaptor protein Nck links receptor tyrosine kinases with the serine-threonine kinase Pak1. The Journal of biological chemistry 241 8798379
2002 The Nck family of adapter proteins: regulators of actin cytoskeleton. Cellular signalling 193 12034353
1992 The SH2 and SH3 domain-containing Nck protein is oncogenic and a common target for phosphorylation by different surface receptors. Molecular and cellular biology 188 1333047
2007 Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. Cell 181 17803907
2001 Nck/Dock: an adapter between cell surface receptors and the actin cytoskeleton. Oncogene 150 11607841
2004 Nck-dependent activation of extracellular signal-regulated kinase-1 and regulation of cell survival during endoplasmic reticulum stress. Molecular biology of the cell 145 15201339
2004 The Traf2- and Nck-interacting kinase as a putative effector of Rap2 to regulate actin cytoskeleton. The Journal of biological chemistry 143 15342639
1998 Nck recruitment to Eph receptor, EphB1/ELK, couples ligand activation to c-Jun kinase. The Journal of biological chemistry 139 9430661
2002 A tyrosine-phosphorylated 12-amino-acid sequence of enteropathogenic Escherichia coli Tir binds the host adaptor protein Nck and is required for Nck localization to actin pedestals. Molecular microbiology 131 11918809
2002 Grb2 and Nck act cooperatively to promote actin-based motility of vaccinia virus. Current biology : CB 125 12007418
2009 Nck adaptor proteins link Tks5 to invadopodia actin regulation and ECM degradation. Journal of cell science 123 19596797
2000 Interaction between PAK and nck: a template for Nck targets and role of PAK autophosphorylation. Molecular and cellular biology 120 10805734
2000 Nck-interacting Ste20 kinase couples Eph receptors to c-Jun N-terminal kinase and integrin activation. Molecular and cellular biology 116 10669731
2010 Traf2- and Nck-interacting kinase is essential for Wnt signaling and colorectal cancer growth. Cancer research 112 20530691
2007 Differential regulation of WASP and N-WASP by Cdc42, Rac1, Nck, and PI(4,5)P2. Biochemistry 108 17302440
1998 A Nck-Pak1 signaling module is required for T-cell receptor-mediated activation of NFAT, but not of JNK. The EMBO journal 105 9755165
1992 The SH2- and SH3-containing Nck protein transforms mammalian fibroblasts in the absence of elevated phosphotyrosine levels. Molecular and cellular biology 101 1280326
2002 The adaptor protein Nck-1 couples the netrin-1 receptor DCC (deleted in colorectal cancer) to the activation of the small GTPase Rac1 through an atypical mechanism. The Journal of biological chemistry 94 12149262
2001 A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT. The EMBO journal 86 11157752
2006 The phosphotyrosine peptide binding specificity of Nck1 and Nck2 Src homology 2 domains. The Journal of biological chemistry 83 16636066
1998 The Nck SH2/SH3 adaptor protein: a regulator of multiple intracellular signal transduction events. BioEssays : news and reviews in molecular, cellular and developmental biology 83 9872057
2018 NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy. Nature communications 71 30150707
1998 Identification of Nck family genes, chromosomal localization, expression, and signaling specificity. The Journal of biological chemistry 70 9737977
2015 Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization. Circulation 66 26659946
2012 Stoichiometry of Nck-dependent actin polymerization in living cells. The Journal of cell biology 66 22613834
2018 LncRNA NCK1-AS1 promotes proliferation and induces cell cycle progression by crosstalk NCK1-AS1/miR-6857/CDK1 pathway. Cell death & disease 52 29416014
2013 Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics. Nature communications 51 24287595
2004 Identification and functional characterization of a novel human misshapen/Nck interacting kinase-related kinase, hMINK beta. The Journal of biological chemistry 51 15469942
1999 Independent SH2-binding sites mediate interaction of Dok-related protein with RasGTPase-activating protein and Nck. The Journal of biological chemistry 51 10428862
2016 NCK Associated Protein 1 Modulated by miRNA-214 Determines Vascular Smooth Muscle Cell Migration, Proliferation, and Neointima Hyperplasia. Journal of the American Heart Association 49 27927633
2013 Cdc42 and the Rho GEF intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation. Journal of cell science 49 24284073
2002 Modulation of protein translation by Nck-1. Proceedings of the National Academy of Sciences of the United States of America 47 11959995
1997 Interaction of Nck-associated protein 1 with activated GTP-binding protein Rac. The Biochemical journal 47 9148763
2010 Nck1 and Grb2 localization patterns can distinguish invadopodia from podosomes. European journal of cell biology 46 20850195
2020 Long non-coding RNA NCK1-AS1 promotes the tumorigenesis of glioma through sponging microRNA-138-2-3p and activating the TRIM24/Wnt/β-catenin axis. Journal of experimental & clinical cancer research : CR 44 32293515
2018 Suppression of long noncoding RNA NCK1-AS1 increases chemosensitivity to cisplatin in cervical cancer. Journal of cellular physiology 43 30221354
2009 Nck- and N-WASP-dependent actin-based motility is conserved in divergent vertebrate poxviruses. Cell host & microbe 43 20006842
2019 Nck-associated protein 1 associates with HSP90 to drive metastasis in human non-small-cell lung cancer. Journal of experimental & clinical cancer research : CR 41 30867003
2013 Nck enables directional cell migration through the coordination of polarized membrane protrusion with adhesion dynamics. Journal of cell science 41 23444376
1999 Nrk: a murine X-linked NIK (Nck-interacting kinase)-related kinase gene expressed in skeletal muscle. Mechanisms of development 40 10559491
2010 Deletion of Nck1 attenuates hepatic ER stress signaling and improves glucose tolerance and insulin signaling in liver of obese mice. American journal of physiology. Endocrinology and metabolism 39 20587749
2006 Rat nephrin modulates cell morphology via the adaptor protein Nck. Biochemical and biophysical research communications 39 16934223
2001 Identification and kinetic analysis of the interaction between Nck-2 and DOCK180. FEBS letters 39 11240126
2008 Structural and functional evidence that Nck interaction with CD3epsilon regulates T-cell receptor activity. Journal of molecular biology 38 18555270
2014 Relevance of Nck-CD3 epsilon interaction for T cell activation in vivo. Journal of immunology (Baltimore, Md. : 1950) 36 24470497
2011 PAK1-Nck regulates cyclin D1 promoter activity in response to prolactin. Molecular endocrinology (Baltimore, Md.) 36 21719533
2010 The adapter protein Nck: role of individual SH3 and SH2 binding modules for protein interactions in T lymphocytes. Protein science : a publication of the Protein Society 36 20082308
2016 Comprehensive Modeling and Discovery of Mebendazole as a Novel TRAF2- and NCK-interacting Kinase Inhibitor. Scientific reports 34 27650168
2015 The Nck-interacting kinase NIK increases Arp2/3 complex activity by phosphorylating the Arp2 subunit. The Journal of cell biology 34 25601402
2014 The cytoplasmic capping complex assembles on adapter protein nck1 bound to the proline-rich C-terminus of Mammalian capping enzyme. PLoS biology 34 25137142
2009 A reciprocal interdependence between Nck and PI(4,5)P(2) promotes localized N-WASp-mediated actin polymerization in living cells. Molecular cell 34 19917259
2006 cGMP-dependent protein kinase phosphorylates p21-activated kinase (Pak) 1, inhibiting Pak/Nck binding and stimulating Pak/vasodilator-stimulated phosphoprotein association. The Journal of biological chemistry 34 16490784
2004 Nck and Crk mediate distinct VEGF-induced signaling pathways that serve overlapping functions in focal adhesion turnover and integrin activation. Experimental cell research 34 15051508
2002 Nck-2 interacts with focal adhesion kinase and modulates cell motility. The international journal of biochemistry & cell biology 34 11950595
1999 Characterization of interactions of Nck with Sos and dynamin. Cellular signalling 34 10206341
2012 Nck and Cdc42 co-operate to recruit N-WASP to promote FcγR-mediated phagocytosis. Journal of cell science 32 22454526
2017 Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis. Cellular signalling 31 28455144
2019 Knockdown Of lncRNA NCK-AS1 Regulates Cisplatin Resistance Through Modulating miR-137 In Osteosarcoma Cells. OncoTargets and therapy 30 31908475
2013 Interaction of Nck1 and PERK phosphorylated at Y⁵⁶¹ negatively modulates PERK activity and PERK regulation of pancreatic β-cell proinsulin content. Molecular biology of the cell 30 24371088
2000 The SH2 and SH3 adapter Nck: a two-gene family and a linker between tyrosine kinases and multiple signaling networks. Histology and histopathology 30 10963137
2019 NCK1-AS1 Increases Drug Resistance of Glioma Cells to Temozolomide by Modulating miR-137/TRIM24. Cancer biotherapy & radiopharmaceuticals 29 31750728
2010 Traf2- and Nck-interacting kinase is essential for canonical Wnt signaling in Xenopus axis formation. The Journal of biological chemistry 29 20566648
2013 Integration of signaling and cytoskeletal remodeling by Nck in directional cell migration. Bioarchitecture 28 23887203
2011 Dock/Nck facilitates PTP61F/PTP1B regulation of insulin signalling. The Biochemical journal 28 21707536
2001 Regulation of Cbl phosphorylation by the Abl tyrosine kinase and the Nck SH2/SH3 adaptor. Oncogene 28 11494134
2020 NCK1-AS1 enhances glioma cell proliferation, radioresistance and chemoresistance via miR-22-3p/IGF1R ceRNA pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 27 32887025
2002 Dok-related protein negatively regulates T cell development via its RasGTPase-activating protein and Nck docking sites. The Journal of cell biology 27 12093790
2001 Cell adhesion regulates the interaction between Nck and p21-activated kinase. The Journal of biological chemistry 27 11278241
2019 NCK1-AS1 promotes NCK1 expression to facilitate tumorigenesis and chemo-resistance in ovarian cancer. Biochemical and biophysical research communications 26 31761329
2015 Nck influences preosteoblastic/osteoblastic migration and bone mass. Proceedings of the National Academy of Sciences of the United States of America 26 26621720
2014 Non-overlapping functions of Nck1 and Nck2 adaptor proteins in T cell activation. Cell communication and signaling : CCS 26 24670066
2009 Regulation of process retraction and cell migration by EphA3 is mediated by the adaptor protein Nck1. Biochemistry 26 19505147
2003 Regulation of SPIN90 phosphorylation and interaction with Nck by ERK and cell adhesion. The Journal of biological chemistry 26 14559906
1994 Chromosome locations of genes encoding human signal transduction adapter proteins, Nck (NCK), Shc (SHC1), and Grb2 (GRB2). Genomics 26 7806213
2010 Nck adaptors are positive regulators of the size and sensitivity of the T-cell repertoire. Proceedings of the National Academy of Sciences of the United States of America 25 20709959
2015 Nck Binds to the T Cell Antigen Receptor Using Its SH3.1 and SH2 Domains in a Cooperative Manner, Promoting TCR Functioning. Journal of immunology (Baltimore, Md. : 1950) 23 26590318
1998 Characterization of Cbl-Nck and Nck-Pak1 interactions in myeloid FcgammaRII signaling. Experimental cell research 23 9851874
2019 Nck adapter proteins promote podosome biogenesis facilitating extracellular matrix degradation and cancer invasion. Cancer medicine 22 31638742
2019 Entry by multiple picornaviruses is dependent on a pathway that includes TNK2, WASL, and NCK1. eLife 22 31769754
2018 Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis. Molecular & cellular proteomics : MCP 22 30002203
2018 NCK1 promotes the angiogenesis of cervical squamous carcinoma via Rac1/PAK1/MMP2 signal pathway. Gynecologic oncology 22 30442385
2020 Multivalent nephrin-Nck interactions define a threshold for clustering and tyrosine-dependent nephrin endocytosis. Journal of cell science 21 31974115
2020 Selective role of Nck1 in atherogenic inflammation and plaque formation. The Journal of clinical investigation 21 32427580
2020 Pharmacological blockage of transforming growth factor-β signalling by a Traf2- and Nck-interacting kinase inhibitor, NCB-0846. British journal of cancer 21 33244122
2016 Traf2- and Nck-interacting kinase (TNIK) is involved in the anti-cancer mechanism of dovitinib in human multiple myeloma IM-9 cells. Amino acids 21 26995282
2015 Requisite role for Nck adaptors in cardiovascular development, endothelial-to-mesenchymal transition, and directed cell migration. Molecular and cellular biology 21 25691664
2015 Nck1 deficiency improves pancreatic β cell survival to diabetes-relevant stresses by modulating PERK activation and signaling. Cellular signalling 21 26434994
2004 The adaptor protein Nck1 mediates endothelin A receptor-regulated cell migration through the Cdc42-dependent c-Jun N-terminal kinase pathway. The Journal of biological chemistry 21 15187089
2011 Identification of Dermcidin as a novel binding protein of Nck1 and characterization of its role in promoting cell migration. Biochimica et biophysica acta 20 21397687
2009 Non-compensating roles between Nckalpha and Nckbeta in PDGF-BB signaling to promote human dermal fibroblast migration. The Journal of investigative dermatology 20 19242519
2021 LncRNA NCK1-AS1 exerts oncogenic property in gastric cancer by targeting the miR-22-3p/BCL9 axis to activate the Wnt/β-catenin signaling. Environmental toxicology 19 33974352
2021 miR-374a-5p inhibits non-small cell lung cancer cell proliferation and migration via targeting NCK1. Experimental and therapeutic medicine 19 34306207
2020 LncRNA NCK1-AS1 Promotes Cancer Cell Proliferation and Increase Cell Stemness in Urinary Bladder Cancer Patients by Downregulating miR-143. Cancer management and research 19 32184669
2005 Dissecting Nck/Dock signaling pathways in Drosophila visual system. International journal of biological sciences 19 15951852
2020 Feasibility of Targeting Traf2-and-Nck-Interacting Kinase in Synovial Sarcoma. Cancers 18 32429395
2012 A neuronal transmembrane protein LRFN4 complexes with 14-3-3s and NCK1 to induce morphological change in monocytic cells via Rac1-mediated actin cytoskeleton reorganization. FEBS letters 18 22677168
2021 Long non-coding RNA PVT1/microRNA miR-3127-5p/NCK-associated protein 1-like axis participates in the pathogenesis of abdominal aortic aneurysm by regulating vascular smooth muscle cells. Bioengineered 17 34898354
2020 LncRNA NCK1-AS1 in plasma distinguishes oral ulcer from early-stage oral squamous cell carcinoma. Journal of biological research (Thessalonike, Greece) 16 33194849

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