Affinage

NBR1

Next to BRCA1 gene 1 protein · UniProt Q14596

Length
966 aa
Mass
107.4 kDa
Annotated
2026-04-29
100 papers in source corpus 22 papers cited in narrative 21 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NBR1 is a multidomain selective autophagy receptor and PB1-domain scaffold that links ubiquitinated cargo to autophagic degradation and transduces signals through MAPK and JNK pathways. Its UBA domain binds K48- and K63-linked polyubiquitin chains while its LIR motif engages LC3/GABARAP proteins on autophagosomes, enabling p62-independent clearance of protein aggregates; it cooperates with p62 by contributing a high-affinity UBA domain to p62–NBR1 heterooligomeric ubiquitin condensates and recruits TAX1BP1 to these condensates for FIP200-dependent autophagic turnover (PMID:19250911, PMID:34471133). NBR1 is the primary receptor for pexophagy, where coincident binding of its amphipathic J domain and UBA domain confers peroxisome selectivity, and it also mediates autophagy-dependent focal adhesion disassembly and selective degradation of ubiquitinated MAVS during viral immune evasion (PMID:23239026, PMID:27484104, PMID:33577621). Beyond autophagy, NBR1 acts as a PB1-domain adaptor that complexes with MEKK3 to activate JNK in macrophages, scaffolds p38 MAPK signaling in osteoblasts, directs Th2 differentiation, and facilitates unconventional IL-12 secretion via late endosomes; its cytosolic accumulation upon autophagy blockade drives pro-metastatic basal differentiation in breast cancer through LLPS-mediated RAC1 degradation (PMID:25043814, PMID:20616007, PMID:20808283, PMID:32084360, PMID:37847732).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2005 High

    Determining the crystal structure of the NBR1 PB1 domain established that NBR1 is a type-A PB1 scaffold capable of binding titin kinase and p62, providing the first structural basis for its signaling adaptor function.

    Evidence X-ray crystallography at 1.55 Å resolution

    PMID:16376336

    Open questions at the time
    • Functional consequences of PB1-mediated interactions in cells were not yet tested
    • Stoichiometry and regulation of the titin kinase–NBR1–p62 complex unresolved
  2. 2009 High

    Identification of NBR1 as an autophagy receptor — with both LIR and UBA domains — answered whether mammals possessed selective autophagy receptors beyond p62 and showed that NBR1 can function independently of p62 to clear ubiquitinated aggregates.

    Evidence Co-IP, domain mutagenesis, autophagic flux assays, siRNA/KO in mammalian cells

    PMID:19250911 PMID:19427866

    Open questions at the time
    • Relative contributions of NBR1 versus p62 to different selective autophagy pathways were unclear
    • Cargo specificity mechanism not yet defined
  3. 2010 High

    A series of genetic studies revealed that NBR1 functions as a PB1-domain adaptor in non-autophagy signaling: it complexes with activated p38 MAPK to restrain osteoblast differentiation, regulates Th2 polarization via Gata3/NFAT, directs FGF receptor trafficking to lysosomes through Spred2, and modulates RTK internalization via a C-terminal amphipathic α-helix.

    Evidence Nbr1-truncation mice (osteoblast phenotype), T-cell-specific Nbr1 KO mice (Th2 defect), Spred2 co-IP and FGF signaling assays, RTK degradation assays with domain mutagenesis

    PMID:19822672 PMID:20616007 PMID:20808283 PMID:20937771

    Open questions at the time
    • How autophagy-receptor and signaling-scaffold functions of NBR1 are coordinated or segregated remained unknown
    • Direct structural basis for p38 MAPK interaction not resolved
  4. 2011 High

    The NMR structure of the GABARAPL-1–NBR1 LIR complex revealed an atypical LIR motif (tyrosine/isoleucine substitutions) and showed that binding affinity is modulated by enthalpy–entropy compensation, providing the molecular basis for how NBR1 engages the autophagosome membrane.

    Evidence NMR structure determination, ITC/fluorescence binding measurements, LIR mutagenesis

    PMID:21620860

    Open questions at the time
    • Relative selectivity of NBR1 LIR for different LC3/GABARAP paralogs in vivo was not established
  5. 2012 High

    Demonstrating that NBR1 is necessary and sufficient for pexophagy — with cargo selectivity arising from coincident J-domain and UBA-domain recognition of peroxisomes — established pexophagy as NBR1's primary selective autophagy pathway and defined a multi-domain coincidence detection mechanism.

    Evidence Domain mutagenesis, pexophagy assays, NBR1 overexpression/knockdown, colocalization imaging

    PMID:23239026

    Open questions at the time
    • Peroxisomal ligand recognized by the J domain was not identified
    • Regulation of pexophagy by post-translational modifications of NBR1 unknown
  6. 2014 High

    Multiple studies clarified NBR1 regulation and additional signaling roles: GSK3 phosphorylation at Thr586 inhibits ubiquitinated aggregate formation, coxsackievirus proteases generate dominant-negative NBR1 fragments to subvert selective autophagy, NBR1–MEKK3 PB1-domain interaction activates JNK in macrophages to drive adipose inflammation, and Ack1 diverts EGFR into an NBR1/p62-dependent degradative pathway.

    Evidence In vitro kinase assays with site-directed mutagenesis and human muscle biopsies (GSK3); viral protease cleavage assays and dominant-negative overexpression (coxsackievirus); myeloid-specific KO mice with JNK activation assays (MEKK3); co-IP and EGFR trafficking assays (Ack1)

    PMID:24413169 PMID:24769734 PMID:24879152 PMID:25043814

    Open questions at the time
    • Whether GSK3-mediated phosphorylation regulates pexophagy specifically was not tested
    • Whether viral cleavage of NBR1 occurs in vivo during natural infection is unclear
    • Ack1–NBR1 interaction lacks reciprocal validation outside a single lab
  7. 2015 Medium

    Ruling out NBR1 (alone or with SQSTM1) from parkin-mediated mitophagy defined a negative boundary for NBR1's selectivity, establishing that its receptor function is pathway-specific rather than universal.

    Evidence NBR1/SQSTM1 single and double KO cells, mitophagy assays after mitochondrial depolarization

    PMID:26512954

    Open questions at the time
    • Whether NBR1 participates in parkin-independent mitophagy pathways was not tested
  8. 2016 Medium

    Identification of NBR1 as a mediator of autophagy-dependent focal adhesion disassembly expanded its repertoire of selective autophagy cargoes beyond protein aggregates and peroxisomes to include cell adhesion structures governing migration.

    Evidence NBR1 knockdown/knockout, focal adhesion turnover assays, live-cell imaging, migration assays

    PMID:27484104

    Open questions at the time
    • Direct ubiquitin signal on focal adhesion components recognized by NBR1 not identified
    • Awaits independent replication
  9. 2020 High

    Showing that autophagy-mediated degradation of NBR1 suppresses breast cancer metastasis — and that accumulated NBR1 drives pro-metastatic basal differentiation — established NBR1 as a critical effector linking autophagy status to tumor cell plasticity.

    Evidence Conditional autophagy KO, transcriptomic analysis, NBR1 overexpression/knockdown, in vivo mammary tumor metastasis models

    PMID:32084360 PMID:32267786

    Open questions at the time
    • Transcriptional targets downstream of accumulated NBR1 that drive basal differentiation not fully mapped
    • Whether this mechanism extends beyond breast cancer models is unknown
  10. 2021 High

    In vitro reconstitution defined the precise division of labor among autophagy receptors: NBR1 equips p62 heterooligomers with a high-affinity UBA domain for condensate nucleation and recruits TAX1BP1, which is the principal driver of FIP200 recruitment and autophagic flux. Separately, cryo-EM of fission yeast Nbr1-ZZ1 revealed bispecific cargo recognition by a single ZZ domain, and NBR1 was shown to deliver ubiquitinated MAVS to autophagosomes during influenza immune evasion.

    Evidence In vitro reconstitution of ubiquitin condensates and KO cell rescue (p62–NBR1–TAX1BP1); cryo-EM of Nbr1-ZZ1 with two cargos; co-IP and IFN induction assays (MAVS degradation)

    PMID:33577621 PMID:34169534 PMID:34471133

    Open questions at the time
    • Whether TAX1BP1 recruitment by NBR1 occurs in all NBR1-dependent selective autophagy pathways is untested
    • ZZ-domain bispecific recognition shown in yeast ortholog — conservation in mammalian NBR1 not demonstrated
  11. 2022 Medium

    Discovery that NBR1 mediates unconventional IL-12 secretion via late endosomes in Atg5-deficient myeloid cells revealed a non-autophagic trafficking function that prevents intestinal dysbiosis and excess inflammation.

    Evidence Myeloid-specific Atg5 KO mice, NBR1 functional assays, IL-12 secretion and late endosome pH measurements

    PMID:34374750

    Open questions at the time
    • Molecular mechanism of NBR1-mediated IL-12 sorting to late endosomes not defined
    • Awaits independent replication
  12. 2023 High

    Demonstrating that TKI-induced lysosomal stress triggers p62/NBR1 LLPS droplets that accelerate RAC1 degradation via cIAP1 explained how NBR1 phase separation connects autophagy receptor condensation to anti-metastatic signaling.

    Evidence p62/NBR1 double KO cells, live-cell LLPS imaging, RAC1 degradation assays, in vivo metastasis models

    PMID:37847732

    Open questions at the time
    • Whether LLPS of NBR1 is relevant to its other selective autophagy functions (pexophagy, aggrephagy) is unknown
    • cIAP1 recruitment mechanism to p62/NBR1 droplets not structurally resolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the identity of the peroxisomal J-domain ligand, how NBR1's autophagy-receptor and signaling-scaffold functions are spatiotemporally segregated, whether mammalian NBR1 ZZ domain exhibits bispecific cargo recognition like its yeast ortholog, and the structural basis for NBR1 phase separation with p62.
  • Peroxisomal ligand for J domain unknown
  • No structural model of full-length mammalian NBR1
  • Regulation of switch between autophagy-receptor and signaling-scaffold modes undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4 GO:0008289 lipid binding 3 GO:0098772 molecular function regulator activity 3
Localization
GO:0005768 endosome 3 GO:0005829 cytosol 3 GO:0031410 cytoplasmic vesicle 3
Pathway
R-HSA-9612973 Autophagy 5 R-HSA-162582 Signal Transduction 4 R-HSA-168256 Immune System 3 R-HSA-392499 Metabolism of proteins 2
Partners
GABARAPL1GSK3BMAP3K3SPRED2SQSTM1TAX1BP1TNK2
Complex memberships
NBR1-MEKK3 PB1 complexp62-NBR1 heterooligomer

Evidence

Reading pass · 21 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2009 NBR1 functions as an autophagy receptor containing both an LC3-interacting region (LIR) and a ubiquitin-binding (UBA) domain, enabling it to link ubiquitinated protein aggregates to autophagosomal degradation. NBR1 is recruited to Ub-positive protein aggregates and degraded by autophagy in an LIR-dependent manner. Although NBR1 and p62 interact and form oligomers, they can function independently—NBR1 mediates autophagosomal clearance in p62-deficient cells. Co-immunoprecipitation, domain mutagenesis, cell depletion (siRNA/knockout), immunofluorescence, autophagic flux assays Molecular cell High 19250911
2009 NBR1 UBA domain binds K48- and K63-linked polyubiquitin chains. NBR1 also binds autophagic effector LC3-A via a novel LIR binding site. Ubiquitin-binding (but not PB1-mediated p62 interaction) is required to target NBR1 to LC3- and polyubiquitin-positive bodies. NBR1 additionally binds ubiquitin-specific peptidases USP8 and endosomal transport regulator p14/Robld3. Pulldown assays, domain mutagenesis, co-immunoprecipitation, fluorescence microscopy FEBS letters High 19427866
2011 The structure of the GABARAPL-1/NBR1-LIR complex was determined by NMR, revealing that the NBR1 LIR motif contains a tyrosine and isoleucine substituting the canonical tryptophan and leucine. A tryptophan in the LIR increases binding affinity; substitution with other aromatic residues or additional N-terminal negative charges has little effect due to enthalpy-entropy compensation. NMR structure determination, binding affinity measurements (ITC/fluorescence), LIR mutagenesis Journal of molecular biology High 21620860
2012 NBR1 is necessary and sufficient for pexophagy (selective autophagic degradation of peroxisomes). The amphipathic α-helical J domain, UBA domain, LIR, and coiled-coil domain are all required for pexophagy. Substrate selectivity is achieved by coincident binding of J and UBA domains to peroxisomes. p62 is not required when NBR1 is in excess but increases the efficiency of NBR1-mediated pexophagy via direct binding to NBR1. NBR1 domain mutagenesis, pexophagy assays, knockdown/overexpression, colocalization imaging Journal of cell science High 23239026
2005 Crystal structure of the PB1 domain of NBR1 was determined at 1.55 Å resolution, revealing a type-A PB1 domain with two negatively charged residue clusters. This domain mediates interactions with titin kinase and with p62, placing NBR1 in the titin kinase signaling pathway. X-ray crystallography FEBS letters High 16376336
2010 Genetic truncation of murine Nbr1 leads to increased bone mass through enhanced osteoblast differentiation via hyperactivation of p38 MAPK. Truncated Nbr1 fails to complex with activated p38 MAPK, and pharmacological inhibition of p38 MAPK abrogates the increased osteoblast differentiation. Nbr1 truncation also increases p62 protein expression. Mouse genetic model (Nbr1 truncation), in vitro osteoblast differentiation, pharmacological p38 MAPK inhibition, co-immunoprecipitation Proceedings of the National Academy of Sciences of the United States of America High 20616007
2010 NBR1 promotes down-regulation of FGF receptor signaling by interacting with Spred2 via a direct binding of Spred2 EVH1 domain to NBR1. This interaction redirects activated FGF receptors to the lysosomal degradation pathway. NBR1 colocalizes with Spred2 on late endosomes, and attenuation of FGF signaling by Spred2 is dependent on this interaction. Co-immunoprecipitation, colocalization imaging, siRNA knockdown, FGF signaling assays The Journal of cell biology High 19822672
2010 Ectopic NBR1 expression inhibits ligand-mediated lysosomal degradation of receptor tyrosine kinases (RTKs), probably by inhibiting receptor internalization. Depletion of NBR1 enhances RTK degradation. The C-terminus of Nbr1 is essential but not sufficient for this activity. The C-terminus contains a novel membrane-interacting amphipathic α-helix essential for late endocytic localization. Autophagic and late endocytic localizations of Nbr1 are independent. Ectopic overexpression, siRNA knockdown, truncation mutagenesis, RTK degradation assays, subcellular fractionation/imaging Molecular and cellular biology High 20937771
2014 NBR1 forms a signaling complex with MEKK3 via their PB1 domains, enabling JNK activation in macrophages. Myeloid-specific NBR1 inactivation impairs macrophage M1 polarization and chemotaxis, prevents adipose tissue inflammation, and improves glucose tolerance in obese mice. PB1 domain interaction assays, myeloid-specific knockout mice, JNK activation assays, adipose tissue inflammation phenotyping Cell metabolism High 25043814
2014 GSK3 phosphorylates NBR1 at Thr586. This phosphorylation prevents the formation of ubiquitinated protein aggregates and inhibits their selective autophagic degradation. Reduced NBR1 phosphorylation correlates with increased protein aggregation severity in sporadic inclusion body myositis (sIBM) muscle biopsies. In vitro kinase assay, site-directed mutagenesis (Thr586), protein aggregation assays (puromycin, desmin mutant), Atg7 KO mice, human muscle biopsy analysis Autophagy High 24879152
2014 During coxsackievirus infection, NBR1 is cleaved at two sites by virus-encoded proteases 2Apro and 3Cpro, generating C-terminal fragments that exert dominant-negative effects on selective autophagy by competing with native NBR1/SQSTM1 for LC3 and ubiquitin chain binding. SQSTM1 and NBR1 also positively regulate each other's expression levels. Viral infection, protease cleavage assays, dominant-negative overexpression, siRNA knockdown, co-immunoprecipitation Cell death and differentiation Medium 24769734
2014 Ack1 (non-receptor tyrosine kinase) colocalizes and interacts with NBR1 via a ubiquitin-associated domain interaction. This colocalization is enhanced by ectopic p62/SQSTM1 expression. Ack1 diverts activated EGFR into a non-canonical degradative pathway marked by association with NBR1, p62, and Atg16L. Co-immunoprecipitation, colocalization imaging, Ack1 knockdown, EGFR trafficking assays, deletion mutagenesis Journal of cell science Medium 24413169
2016 NBR1 acts as a critical mediator of selective autophagy-dependent focal adhesion (FA) disassembly, promoting cell migration. NBR1 targets autophagosomes to FAs leading to their disassembly by sequestering FA proteins. NBR1 knockdown/knockout, focal adhesion turnover assays, live-cell imaging, migration assays Autophagy Medium 27484104
2020 Autophagic degradation of NBR1 suppresses breast cancer metastasis. Genetic autophagy inhibition causes cytosolic accumulation of NBR1 in tumor cells, eliciting a pro-metastatic basal epithelial differentiation subpopulation. Preventing NBR1 accumulation reverses this pro-metastatic differentiation and suppresses metastatic outgrowth. Genetic autophagy ablation (conditional KO), transcriptomic analysis, NBR1 overexpression/knockdown, mammary tumor models (in vivo metastasis) Developmental cell High 32084360 32267786
2021 In vitro reconstitution defined the roles of NBR1 in ubiquitin condensate formation: NBR1 promotes condensate formation by equipping the p62-NBR1 heterooligomeric complex with a high-affinity UBA domain. NBR1 also recruits TAX1BP1 to ubiquitin condensates formed by p62. All three receptors interact with FIP200, but TAX1BP1 is the main driver of FIP200 recruitment and autophagic degradation. In vitro reconstitution, condensate formation assays, co-immunoprecipitation, cell biology (KO cell lines) Nature communications High 34471133
2021 Influenza A virus PB1 protein promotes K27-linked polyubiquitination of MAVS (at Lys362 and Lys461) via E3 ligase RNF5, and then preferentially associates with NBR1. NBR1 recognizes ubiquitinated MAVS and delivers it to autophagosomes for degradation, suppressing RIG-I-MAVS innate immune signaling. Co-immunoprecipitation, ubiquitination assays (K27-linked), siRNA knockdown of NBR1, IFN induction assays PLoS pathogens Medium 33577621
2021 Cryo-EM structure of the ZZ domain of fission yeast Nbr1 (Nbr1-ZZ1) in complex with two distinct cargos (Ams1 and Ape4) reveals that a single ZZ domain can recognize two different protein cargos: via a conserved acidic pocket recognizing N-termini of cargos, and via additional cargo-specific contacts. This establishes a single-domain bispecific cargo recognition mechanism in selective autophagy. Cryo-EM structure determination, cargo competition assays, binding analysis The EMBO journal High 34169534
2010 T-cell-specific NBR1-deficient mice display impaired lung inflammation and defective Th2 differentiation ex vivo, with alterations in T-cell polarity and selective inhibition of Gata3 and NFAT activation, establishing NBR1 as a novel PB1 adapter in Th2 differentiation and allergic airway inflammation. T-cell-specific NBR1 knockout mice, ex vivo T-cell differentiation assays, lung inflammation models The EMBO journal High 20808283
2015 NBR1 is dispensable for PARK2 (parkin)-mediated mitophagy, both alone and in combination with SQSTM1 deletion. Deletion of NBR1 alone or together with SQSTM1 does not prevent degradation of damaged mitochondria or mitochondrial clustering after depolarization. NBR1/SQSTM1 single and double KO cells, mitophagy assays (mitochondrial depolarization), flow cytometry Cell death & disease Medium 26512954
2023 Lysosomal stress induced by tyrosine kinase inhibitors promotes liquid-liquid phase separation (LLPS) of p62 and NBR1 into liquid droplets. Within these droplets, RAC1 (Ras-related C3 botulinum toxin substrate 1) degradation is accelerated by cIAP1, limiting cancer cell motility. The antimetastatic activity of TKIs is abolished in p62/NBR1 double-knockout cells in vitro and in vivo. p62/NBR1 double KO cells, live-cell imaging of phase separation, RAC1 degradation assays, in vivo metastasis models Proceedings of the National Academy of Sciences of the United States of America High 37847732
2022 NBR1 plays a non-autophagy role in unconventional secretion of IL-12: in Atg5-deficient myeloid cells, NBR1 accumulates and delivers IL-12 to late endosomes, contributing to excess IL-12 secretion. This pathway prevents dysbiosis and excessive intestinal inflammation. Myeloid-specific Atg5 KO mice, NBR1 functional assays, IL-12 secretion assays, late endosome pH measurements Journal of Crohn's & colitis Medium 34374750

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2009 A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Molecular cell 928 19250911
2012 NBR1 acts as an autophagy receptor for peroxisomes. Journal of cell science 303 23239026
2013 NBR1-mediated selective autophagy targets insoluble ubiquitinated protein aggregates in plant stress responses. PLoS genetics 295 23341779
2005 Potential markers that complement expression of CA125 in epithelial ovarian cancer. Gynecologic oncology 272 16061277
2017 Selective autophagy limits cauliflower mosaic virus infection by NBR1-mediated targeting of viral capsid protein and particles. Proceedings of the National Academy of Sciences of the United States of America 213 28223514
1986 The use of CA-125 in the diagnosis and management of endometriosis. Fertility and sterility 186 3465595
2021 Reconstitution defines the roles of p62, NBR1 and TAX1BP1 in ubiquitin condensate formation and autophagy initiation. Nature communications 177 34471133
2008 A binding domain on mesothelin for CA125/MUC16. The Journal of biological chemistry 157 19075018
2011 MUC16 (CA125) regulates epithelial ovarian cancer cell growth, tumorigenesis and metastasis. Gynecologic oncology 150 21421261
2014 E3 ubiquitin ligase CHIP and NBR1-mediated selective autophagy protect additively against proteotoxicity in plant stress responses. PLoS genetics 134 24497840
2003 The cancer antigen CA125 represents a novel counter receptor for galectin-1. Journal of cell science 127 12615972
2021 The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradation. PLoS pathogens 119 33577621
2020 Selective autophagy regulates heat stress memory in Arabidopsis by NBR1-mediated targeting of HSP90.1 and ROF1. Autophagy 105 32967551
2020 Autophagy suppresses breast cancer metastasis by degrading NBR1. Autophagy 103 32267786
2020 Autophagic Degradation of NBR1 Restricts Metastatic Outgrowth during Mammary Tumor Progression. Developmental cell 101 32084360
2020 Arabidopsis cargo receptor NBR1 mediates selective autophagy of defective proteins. Journal of experimental botany 88 31494674
2011 Co-expression of mesothelin and CA125 correlates with unfavorable patient outcome in pancreatic ductal adenocarcinoma. Pancreas 88 21775916
2015 Understanding the Unique Attributes of MUC16 (CA125): Potential Implications in Targeted Therapy. Cancer research 78 26527287
2009 Interactions with LC3 and polyubiquitin chains link nbr1 to autophagic protein turnover. FEBS letters 78 19427866
2011 Characterization of the interaction of GABARAPL-1 with the LIR motif of NBR1. Journal of molecular biology 76 21620860
2022 NBR1: The archetypal selective autophagy receptor. The Journal of cell biology 75 36255390
2020 Brassinosteroids act as a positive regulator of NBR1-dependent selective autophagy in response to chilling stress in tomato. Journal of experimental botany 71 31639824
2010 Neighbor of Brca1 gene (Nbr1) functions as a negative regulator of postnatal osteoblastic bone formation and p38 MAPK activity. Proceedings of the National Academy of Sciences of the United States of America 71 20616007
2014 Gambogic acid induced oxidative stress dependent caspase activation regulates both apoptosis and autophagy by targeting various key molecules (NF-κB, Beclin-1, p62 and NBR1) in human bladder cancer cells. Biochimica et biophysica acta 67 25218692
2004 CA125- and tumor-specific T-cell responses correlate with prolonged survival in oregovomab-treated recurrent ovarian cancer patients. Gynecologic oncology 66 15297171
2011 Evaluation of ovarian cancer biomarkers HE4 and CA-125 in women presenting with a suspicious cystic ovarian mass. Journal of gynecologic oncology 65 22247801
2013 p38(MAPK)-regulated induction of p62 and NBR1 after photodynamic therapy promotes autophagic clearance of ubiquitin aggregates and reduces reactive oxygen species levels by supporting Nrf2-antioxidant signaling. Free radical biology & medicine 64 24269898
1997 Immunohistochemical phenotype of malignant mesothelioma: predictive value of CA125 and HBME-1 expression. Histopathology 64 9023557
1998 Endometrial protein PP14 and CA-125 in recurrent miscarriage patients; correlation with pregnancy outcome. Human reproduction (Oxford, England) 61 9853880
2012 Pathobiological implications of MUC16/CA125 expression in intrahepatic cholangiocarcinoma-mass forming type. Pathobiology : journal of immunopathology, molecular and cellular biology 58 22286058
2011 CA125 (MUC16) gene silencing suppresses growth properties of ovarian and breast cancer cells. European journal of cancer (Oxford, England : 1990) 58 21852110
2007 Development and in vitro validation of anti-mesothelin biobodies that prevent CA125/Mesothelin-dependent cell attachment. Cancer letters 57 17560019
2002 Tissue and serum CA125 expression in endometrial cancer. International journal of gynecological cancer : official journal of the International Gynecological Cancer Society 53 12144685
2014 Dominant-negative function of the C-terminal fragments of NBR1 and SQSTM1 generated during enteroviral infection. Cell death and differentiation 51 24769734
2011 Abnormalities of NBR1, a novel autophagy-associated protein, in muscle fibers of sporadic inclusion-body myositis. Acta neuropathologica 50 21935636
2017 Oncogenic KRAS Targets MUC16/CA125 in Pancreatic Ductal Adenocarcinoma. Molecular cancer research : MCR 48 28108627
2002 Diagnostic laparoscopy, serum CA125, and peritoneal metastasis in gastric cancer. Endoscopy 47 12170412
2014 Phosphorylation of NBR1 by GSK3 modulates protein aggregation. Autophagy 46 24879152
2013 Brain region- and age-dependent dysregulation of p62 and NBR1 in a mouse model of Huntington's disease. Neurobiology of disease 45 23295856
2014 A macrophage NBR1-MEKK3 complex triggers JNK-mediated adipose tissue inflammation in obesity. Cell metabolism 44 25043814
2012 Interpretation of single and serial measures of HE4 and CA125 in asymptomatic women at high risk for ovarian cancer. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 44 22962406
2009 Spred2 interaction with the late endosomal protein NBR1 down-regulates fibroblast growth factor receptor signaling. The Journal of cell biology 44 19822672
2018 Comparison of CA125, HE4, and ROMA index for ovarian cancer diagnosis. Current problems in cancer 42 30017407
2017 Smartphone-based immunosensor for CA125 detection. Talanta 42 28213228
2014 HE4, Ca125 and ROMA algorithm for differential diagnosis between benign gynaecological diseases and ovarian cancer. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 42 24771264
2010 Nbr1 is a novel inhibitor of ligand-mediated receptor tyrosine kinase degradation. Molecular and cellular biology 42 20937771
2022 The Potential Role of MUC16 (CA125) Biomarker in Lung Cancer: A Magic Biomarker but with Adversity. Diagnostics (Basel, Switzerland) 41 36552994
2014 CEA, AFP, CA125, CA153 and CA199 in malignant pleural effusions predict the cause. Asian Pacific journal of cancer prevention : APJCP 41 24528057
2015 Expression of the Carboxy-Terminal Portion of MUC16/CA125 Induces Transformation and Tumor Invasion. PloS one 40 25965947
2020 Broad and Complex Roles of NBR1-Mediated Selective Autophagy in Plant Stress Responses. Cells 37 33266087
2021 Consideration should be given to smoking, endometriosis, renal function (eGFR) and age when interpreting CA125 and HE4 in ovarian tumor diagnostics. Clinical chemistry and laboratory medicine 36 34388324
2015 NBR1 is dispensable for PARK2-mediated mitophagy regardless of the presence or absence of SQSTM1. Cell death & disease 34 26512954
2023 LLPS of SQSTM1/p62 and NBR1 as outcomes of lysosomal stress response limits cancer cell metastasis. Proceedings of the National Academy of Sciences of the United States of America 33 37847732
2020 The Combination of CA125 and NSE Is Useful for Predicting Liver Metastasis of Lung Cancer. Disease markers 33 33376560
2014 The non-receptor tyrosine kinase Ack1 regulates the fate of activated EGFR by inducing trafficking to the p62/NBR1 pre-autophagosome. Journal of cell science 33 24413169
2012 Autophagy-related proteins (p62, NBR1 and LC3) in intranuclear inclusions in neurodegenerative diseases. Neuroscience letters 32 22728060
2012 Deciphering the molecular nature of ovarian cancer biomarker CA125. International journal of molecular sciences 32 22949880
2020 A selective autophagy cargo receptor NBR1 modulates abscisic acid signalling in Arabidopsis thaliana. Scientific reports 31 32385330
2018 Suitability assessment of baseline concentration of MMP3, TIMP3, HE4 and CA125 in the serum of patients with ovarian cancer. Journal of ovarian research 31 29304854
2015 Diagnostic performances of CA125, HE4, and ROMA index in ovarian cancer. European journal of gynaecological oncology 30 26390703
2010 NBR1 is a new PB1 signalling adapter in Th2 differentiation and allergic airway inflammation in vivo. The EMBO journal 30 20808283
2009 CA125/MUC16 is dispensable for mouse development and reproduction. PloS one 30 19262696
1996 The BRCA1 and 1A1.3B promoters are parallel elements of a genomic duplication at 17q21. Genomics 30 8954804
2023 The autophagy receptor NBR1 directs the clearance of photodamaged chloroplasts. eLife 29 37070813
2002 Use of CA-125 and ultrasound in high-risk women. International journal of gynecological cancer : official journal of the International Gynecological Cancer Society 29 11860541
2021 The diagnostic value of the combination of hemoglobin, CA199, CA125, and HE4 in endometriosis. Journal of clinical laboratory analysis 28 34405450
2009 CA-125 and CRP are elevated in preeclampsia. Hypertension in pregnancy 27 19437230
2024 Enteric coronavirus nsp2 is a virulence determinant that recruits NBR1 for autophagic targeting of TBK1 to diminish the innate immune response. Autophagy 26 38597182
2019 Diagnostic accuracy of CA125 and HE4 in ovarian carcinoma patients and the effect of confounders on their serum levels. Current problems in cancer 24 30670303
2017 CA-125 in Disease Progression and Treatment of Lymphangioleiomyomatosis. Chest 24 28576630
2023 In silico designed mRNA vaccines targeting CA-125 neoantigen in breast and ovarian cancer. Vaccine 23 36813666
2021 Poplar Autophagy Receptor NBR1 Enhances Salt Stress Tolerance by Regulating Selective Autophagy and Antioxidant System. Frontiers in plant science 23 33552091
2016 Expression of CEA, CA19-9, CA125, and EpCAM in pseudomyxoma peritonei. Human pathology 23 27038681
2022 HE4 and CA125 serum biomarker monitoring in women with epithelial ovarian cancer. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 22 36189508
2007 The expression of Wilms' tumour-1 and Ca125 in invasive micropapillary carcinoma of the breast. Histopathology 22 18042071
2018 NBR1 is involved in selective pexophagy in filamentous ascomycetes and can be functionally replaced by a tagged version of its human homolog. Autophagy 21 30081713
1996 Isolation of the murine Nbr1 gene adjacent to the murine Brca1 gene. Genomics 21 8975707
1988 Correlation of an enzyme immunoassay with the radioimmunoassay for CA-125. American journal of obstetrics and gynecology 21 3276202
2021 Molecular and structural mechanisms of ZZ domain-mediated cargo selection by Nbr1. The EMBO journal 20 34169534
2020 Overexpression of the Selective Autophagy Cargo Receptor NBR1 Modifies Plant Response to Sulfur Deficit. Cells 20 32164165
2023 PSMB1 Inhibits the Replication of Porcine Reproductive and Respiratory Syndrome Virus by Recruiting NBR1 To Degrade Nonstructural Protein 12 by Autophagy. Journal of virology 19 36602366
2021 Serum HE4 and CA125 combined to predict and monitor recurrence of type II endometrial carcinoma. Scientific reports 19 34737393
2013 HE4 combined with CA125: favorable screening tool for ovarian cancer. Medical oncology (Northwood, London, England) 19 24323399
2011 Pre-treatment diagnosis of endometrial cancer through a combination of CA125 and multiplication of neutrophil and monocyte. The journal of obstetrics and gynaecology research 19 22142582
2016 NBR1-dependent selective autophagy is required for efficient cell-matrix adhesion site disassembly. Autophagy 18 27484104
2010 Conflicting views on the molecular structure of the cancer antigen CA125/MUC16. Disease markers 18 20683153
2009 Premenarchal ovarian torsion and elevated CA-125. Journal of pediatric and adolescent gynecology 18 19589703
2005 Crystal structure of the PB1 domain of NBR1. FEBS letters 18 16376336
2021 Early diagonosis of ovarian cancer: serum HE4, CA125 and ROMA model. American journal of translational research 17 35035759
2013 Saliva CA125 and TPS levels in patients with oral squamous cell carcinoma. The International journal of biological markers 17 23613350
2011 Combination of CA125 and RECAF biomarkers for early detection of ovarian cancer. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 17 21625941
2010 Autophagic receptors Nbr1 and p62 coregulate skeletal remodeling. Autophagy 17 20814249
2023 Diagnostic value of the combination of circulating serum miRNAs and CA125 in endometriosis. Medicine 16 38050316
2021 Significance of mesothelin and CA125 expression in endometrial carcinoma: a retrospective analysis. Diagnostic pathology 16 33832498
2022 Non-autophagy Role of Atg5 and NBR1 in Unconventional Secretion of IL-12 Prevents Gut Dysbiosis and Inflammation. Journal of Crohn's & colitis 15 34374750
2022 Ovarian Cancer Ascites Inhibits Transcriptional Activation of NK Cells Partly through CA125. Journal of immunology (Baltimore, Md. : 1950) 15 35396222
2019 Heme acquisition by Shu1 requires Nbr1 and proteins of the ESCRT complex in Schizosaccharomyces pombe. Molecular microbiology 14 31442344
2022 Urine CA125 and HE4 for the Triage of Symptomatic Women with Suspected Endometrial Cancer. Cancers 13 35884367
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2001 Expression of BRCA1, NBR1 and NBR2 genes in human breast cancer cells. Folia biologica 12 11508855