Affinage

MOB1A

MOB kinase activator 1A · UniProt Q9H8S9

Length
216 aa
Mass
25.1 kDa
Annotated
2026-06-10
69 papers in source corpus 30 papers cited in narrative 30 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/8 claims corpus-supported (88%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MOB1A is a conserved kinase coactivator and phosphorylation-controlled scaffold that operates as a core hub of the Hippo tumor-suppressor pathway and the mitotic exit/cytokinesis machinery (PMID:26108669, PMID:26699479). The protein folds as a zinc-stabilized four-helix bundle whose conserved, electrostatically negative surface engages basic regulatory regions of partner kinases (PMID:12962634, PMID:15001360). In the activation cycle, autophosphorylated MST1/2 present docking phosphomotifs that MOB1A recognizes through a dedicated phosphopeptide-binding surface, and MST-mediated phosphorylation of MOB1A (e.g. Thr12/Thr35) relieves an N-terminal Switch-helix autoinhibition by a 'pull-the-string' mechanism, exposing a distinct surface for LATS/NDR kinase binding (PMID:26108669, PMID:27335147, PMID:28373298); through dynamic scaffolding and allostery MOB1A then activates LATS1/2 and NDR kinases (PMID:16674920, PMID:18362890, PMID:26108669). The MOB1-LATS(Warts) interaction is the binding event essential for tumor suppression and growth control, whereas stable MOB1-MST binding is dispensable and MOB1-NDR binding alone is insufficient (PMID:28947795). Genetically, MOB1A/B act as the critical node upstream of LATS-YAP1/TAZ, since their loss in mouse liver drives YAP1-dependent hyperproliferation, dedifferentiation, EMT, and tumorigenesis (PMID:26699479). MOB1A abundance is tuned by competing post-translational modifications: praja2-mediated ubiquitylation drives proteasomal degradation (PMID:23652010), opposed by CBP/HDAC6-controlled Lys11 acetylation that blocks praja2 binding and stabilizes the protein (PMID:35349706), while GSK3β phosphorylation at Ser146 promotes its degradation (PMID:30069702). This conserved coactivator role originated in the yeast mitotic exit network, where Mob1 binds and activates the Dbf2 kinase—itself activated by Cdc15 downstream of Tem1—and the Dbf2-Mob1 complex phosphorylates RXXS-motif substrates including Cdc14 to control nucleolar release and cytokinesis (PMID:9528782, PMID:11404483, PMID:16242037, PMID:19221193). In human cells MOB1A likewise localizes to spindle poles, kinetochores, and the midzone and is required for cytokinetic abscission and centrosome control (PMID:19955215, PMID:22454515).

Mechanistic history

Synthesis pass · year-by-year structured walk · 22 steps
  1. 1998 High

    Established the founding principle that Mob1 is an obligate binding partner and effector of the Dbf2 kinase in late mitosis, answering how Dbf2 executes its cell-cycle function.

    Evidence Two-hybrid, reciprocal in vivo/in vitro binding, and genetic suppression in budding yeast

    PMID:9436989 PMID:9528782

    Open questions at the time
    • Did not define the catalytic consequence of binding on Dbf2 activity
    • Mammalian ortholog function not addressed
  2. 2001 High

    Defined Mob1's position in the mitotic exit network and showed it is required for an upstream kinase (Cdc15) to phosphorylate and activate Dbf2, establishing Mob1 as a coactivator rather than a passive partner.

    Evidence In vitro kinase reconstitution with phosphoacceptor mutagenesis, genetic epistasis, and localization microscopy in yeast

    PMID:11378390 PMID:11404483 PMID:11434459

    Open questions at the time
    • Mechanism by which Mob1 enables substrate phosphorylation not structurally resolved
    • Restricted to yeast MEN
  3. 2001 Medium

    Connected mammalian MOB1 to phosphatase regulation, showing it associates with striatin-PP2A complexes and is a PP2A dephosphorylation target near the nuclear membrane.

    Evidence Co-IP, pharmacological PP2A inhibition, and immunofluorescence in murine fibroblasts

    PMID:11319234

    Open questions at the time
    • Functional consequence of PP2A association unresolved
    • Single lab, no reciprocal kinase counterpart identified
  4. 2003 High

    Provided the structural basis for MOB1 function, revealing a zinc-stabilized four-helix bundle with a conserved acidic surface predicted to bind basic kinase regions.

    Evidence X-ray crystallography of human MOB1A with mapping to yeast conditional alleles, corroborated by Xenopus NMR with NDR peptide perturbation

    PMID:12962634 PMID:15001360

    Open questions at the time
    • Full-length autoinhibitory elements not yet captured
    • Did not show phosphorylation-dependent conformational change
  5. 2005 High

    Defined the intrinsic substrate specificity of the Dbf2-Mob1 kinase complex as an RXXS consensus, allowing prediction of physiological substrates.

    Evidence Peptide library selection and proteome microarray phosphorylation screening

    PMID:16242037

    Open questions at the time
    • In vitro specificity not directly tied to in vivo substrate set
    • Yeast complex only
  6. 2006 Medium

    Showed human MOB1 activates LATS1 only upon membrane targeting and identified a conserved MOB1-binding motif in LATS1, linking MOB1 to the mammalian Hippo effector kinase.

    Evidence Membrane-targeting co-expression, kinase assays, and mutagenesis in cells

    PMID:16674920

    Open questions at the time
    • Physiological membrane recruitment trigger unclear
    • Single lab
  7. 2008 High

    Identified MST2 phosphorylation of MOB1 at Thr74 as the event nucleating a MOB1-MST2-NDR1 scaffold required for NDR1 activation, establishing a phospho-dependent assembly mechanism in mammals.

    Evidence In vitro kinase assay, T74A mutagenesis, co-IP, and RNAi with NDR1 activity readout

    PMID:18362890

    Open questions at the time
    • Structural basis of the scaffold not resolved at this stage
    • Relationship to LATS branch not addressed
  8. 2009 High

    Identified Cdc14 as a direct Dbf2-Mob1 substrate, showing phosphorylation adjacent to its NLS drives nucleolar-to-cytoplasmic relocalization, mechanistically linking the complex to mitotic exit.

    Evidence In vitro kinase assay, MS phosphosite mapping, and NLS localization assays in yeast

    PMID:19221193

    Open questions at the time
    • Mammalian substrate equivalents not defined here
  9. 2009 Medium

    Mapped human MOB1 to mitotic structures and showed its recruitment depends on the CPC and Plk1, implicating it in chromosomal passenger relocalization and cytokinesis.

    Evidence Immunofluorescence, RNAi, and live-cell imaging in human cells

    PMID:19955215

    Open questions at the time
    • Kinase partner driving midzone function not pinpointed
    • Single lab
  10. 2010 Medium

    Established that among seven MOB family members only MOB1A/B bind LATS1/2 and activate LATS-mediated growth suppression, defining MOB1A/B as the LATS-specific coactivators.

    Evidence Co-IP, in vitro binding, kinase assays, and overexpression/shRNA in cancer cells

    PMID:19739119

    Open questions at the time
    • In vivo tumor relevance not yet shown
    • Single lab
  11. 2012 Medium

    Demonstrated MOB1A/B are required for cytokinetic abscission and centrosome control, linking the Hippo coactivator to microtubule dynamics in the midbody.

    Evidence RNAi depletion, microtubule stability assays, and live-cell imaging

    PMID:22454515

    Open questions at the time
    • Effector kinase responsible for midbody microtubule destabilization not identified
    • Single lab
  12. 2013 High

    Identified praja2 as the E3 ligase that ubiquitylates MOB1 for degradation, establishing proteostatic control of Hippo signaling output relevant to tumor growth.

    Evidence Co-IP, ubiquitylation assay, proteasome inhibition, and in vivo glioblastoma growth

    PMID:23652010

    Open questions at the time
    • Counter-regulatory stabilizing mechanisms not yet defined
    • Single lab
  13. 2015 High

    Resolved the phosphorylation-driven activation cycle in atomic detail, showing MOB1 binds autophosphorylated MST2 docking motifs, becomes phosphorylated, and then engages LATS1 via dynamic scaffolding and allostery.

    Evidence Crystal structures of phospho-MST2-MOB1 and phospho-MOB1-LATS1 complexes with biochemical reconstitution

    PMID:26108669

    Open questions at the time
    • Precise autoinhibitory release step not yet captured in full-length structure
  14. 2015 High

    Showed in vivo that MOB1A/B are the critical Hippo hub upstream of YAP1/TAZ, since their liver-specific loss causes YAP1-dependent tumorigenesis.

    Evidence Conditional double-knockout mice with Yap1 epistatic rescue and cell assays

    PMID:26699479

    Open questions at the time
    • Relative roles of MOB1A vs MOB1B not dissected
  15. 2016 High

    Captured the autoinhibited full-length MOB1 conformation and showed MST-mediated Thr12/Thr35 phosphorylation relieves a Switch-helix block on the LATS-binding surface via a pull-the-string mechanism.

    Evidence Crystal structures of autoinhibited MOB1B and its complex with LATS1 NTR plus biochemical validation

    PMID:27335147

    Open questions at the time
    • Single lab; in vivo requirement of the Switch helix not tested in mammals
  16. 2017 High

    Defined how phosphorylation differentially partitions MOB1's two interaction surfaces and expanded its interactome beyond core Hippo kinases to PP6 and DOCK6-8 complexes.

    Evidence Crystal structures, peptide arrays, biophysical binding, and interaction proteomics

    PMID:28373297 PMID:28373298

    Open questions at the time
    • Functional roles of PP6 and DOCK associations not characterized
  17. 2017 High

    Genetically dissected which MOB1 kinase interaction is essential, showing MOB1-LATS/Warts binding underlies tumor suppression while stable MOB1-MST binding is dispensable.

    Evidence Crystal structure of MOB1/NDR2, selective loss-of-interaction variants, Drosophila genetics, and human cells

    PMID:28947795

    Open questions at the time
    • How transient MST binding still contributes catalytically not fully reconciled
  18. 2019 Medium

    Identified upstream regulators of MOB1 turnover and activity (Kindlin-2 enhancing praja2-mediated degradation; PR55α/PP2A inhibiting MOB1-driven LATS autoactivation), linking MOB1 control to fibrosis and cancer.

    Evidence Co-IP, ubiquitylation and kinase assays, RNAi, and mouse disease models

    PMID:31659153 PMID:31825843

    Open questions at the time
    • Single labs; direct vs indirect effects on MOB1 not fully separated
  19. 2018 Medium

    Established GSK3β phosphorylation of MOB1 at Ser146 as a degradation signal under PTEN-PI3K-AKT control, connecting MOB1 stability to neurite outgrowth.

    Evidence In vitro kinase assay, S146 mutagenesis, proteasome assays, and in vivo silencing

    PMID:30069702

    Open questions at the time
    • Single lab; relationship to praja2 pathway not integrated
  20. 2020 High

    Revealed that a bacterial effector kinase LegK7 hijacks MOB1A as both substrate and allosteric activator, with the MOB1A N-terminal extension acting as a YAP1 docking platform, generalizing MOB1's coactivator role.

    Evidence Crystal structure of LegK7-MOB1A, kinase assays, interface mutagenesis, and cell-based phosphorylation

    PMID:32513747

    Open questions at the time
    • Whether the same NTE docking operates with host kinases not established
  21. 2022 High

    Identified competing stabilizing modifications and non-canonical inputs to MOB1: CBP/HDAC6-controlled Lys11 acetylation blocks praja2 ubiquitylation and stabilizes MOB1, while a CAF-exosomal lncRNA scaffolds MOB1A-praja2 to promote its degradation.

    Evidence Acetylation/ubiquitylation assays, K11R mutagenesis, RNA-protein interaction, and in vivo tumor models

    PMID:35349706 PMID:36123327

    Open questions at the time
    • Integration of acetylation, phosphorylation, and ubiquitylation timing not resolved
  22. 2021 Medium

    Showed EGFR drives tyrosine phosphorylation of MOB1 to inactivate LATS1/2 independently of MST1/2 and activate YAP/TAZ, revealing a receptor-driven, MST-independent branch of MOB1 regulation.

    Evidence Phosphorylation assays, erlotinib inhibition, RNAi, and transcriptomics in HNSCC cells

    PMID:34725466

    Open questions at the time
    • Tyrosine phosphosite and direct vs indirect mechanism not mapped
    • Single lab

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the multiple PTMs (MST/GSK3β/EGFR phosphorylation, K11 acetylation, praja2 ubiquitylation) are temporally and spatially integrated to set MOB1A levels and direct it between Hippo signaling and mitotic exit remains unresolved.
  • No unified model coordinating competing modifications
  • Mammalian MOB1A vs MOB1B functional division undefined
  • Physiological cytokinesis substrates of human MOB1 not identified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 6 GO:0060090 molecular adaptor activity 3
Localization
GO:0005815 microtubule organizing center 2 GO:0005886 plasma membrane 2 GO:0005634 nucleus 1
Pathway
R-HSA-1640170 Cell Cycle 5 R-HSA-162582 Signal Transduction 3 R-HSA-392499 Metabolism of proteins 3
Partners
CDC15DBF2LATS1LATS2MST1MST2NDR1PRAJA2
Complex memberships
Dbf2-Mob1 complexMOB1-LATS1/2 complexMST2-MOB1-NDR1 scaffoldstriatin/SG2NA-PP2A complex

Evidence

Reading pass · 30 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 MOB1 (yeast) physically binds DBF2 in vivo and in vitro in the absence of other yeast proteins; this interaction is required for the late mitotic function of DBF2, as temperature-sensitive MOB1 mutants that cannot bind DBF2 fail to execute the telophase/G1 transition. Overexpression of MOB1 suppresses lethality of dbf2Δ dbf20Δ double deletion, indicating DBF2 acts through MOB1. Two-hybrid screen, co-immunoprecipitation, in vitro binding, genetic suppression analysis Molecular and cellular biology High 9528782
1998 Yeast MOB1 is a phosphoprotein in vivo and a substrate for the Mps1p kinase in vitro; loss-of-function mob1 alleles cause late nuclear division arrest, and MOB1 shows genetic interactions with LTE1, CDC5, and CDC15, placing it in the mitotic exit pathway. Two-hybrid screen, in vitro kinase assay, conditional allele analysis, genetic interaction Molecular biology of the cell High 9436989
2001 Cdc15 kinase activates the Dbf2-Mob1 complex in vitro only when Dbf2 is bound to Mob1; Cdc15 phosphorylates conserved sites Ser-374 and Thr-544 on Dbf2, and Mob1 enables Cdc15 to phosphorylate these critical sites. Dbf2 kinase activity in vivo depends on Tem1, Cdc15, and Mob1. In vitro kinase reconstitution, site-directed mutagenesis, in vivo activity assays Proceedings of the National Academy of Sciences of the United States of America High 11404483
2001 Genetic epistasis in budding yeast places Tem1 upstream of Cdc15, which functions upstream of Mob1 and Dbf2 in the mitotic exit network; Dbf2 kinase activity and actin ring formation at the bud neck are both controlled by the same MEN components including Mob1. Genetic epistasis, Dbf2 kinase activity assays, actin ring visualization Current biology : CB High 11378390
2001 Mammalian MOB1 (mMOB1) associates with striatin/SG2NA-PP2A complexes; PP2A dephosphorylates mMOB1 since PP2A inhibition with okadaic acid induces serine phosphorylation of PP2A-associated mMOB1. mMOB1 localizes adjacent to the nuclear membrane in murine fibroblasts. Co-immunoprecipitation, pharmacological PP2A inhibition, indirect immunofluorescence, mass spectrometry identification The Journal of biological chemistry Medium 11319234
2001 Dbf2 and Mob1 (yeast) localize to spindle pole bodies in anaphase and then relocalize to the bud neck just prior to actin ring assembly; bud-neck localization but not SPB localization of Dbf2 is inhibited by the Bub2 spindle checkpoint, and neck localization of both Dbf2 and Mob1 depends on Cdc14 activity. Fluorescence microscopy of tagged proteins, genetic analysis with checkpoint and cdc14 mutants Genes & genetic systems Medium 11434459
2003 Crystal structure of human MOB1A reveals a four-helix bundle stabilized by a bound zinc atom; the N-terminal helix and adjacent elements form an evolutionarily conserved surface with strong negative electrostatic potential that is targeted by temperature-sensitive yeast MOB1 alleles, suggesting this surface mediates electrostatic interactions with basic regions of kinase partners. X-ray crystallography Structure (London, England : 1993) High 12962634
2004 NMR solution structure of Xenopus Mob1 shows a four-helix bundle with a zinc-binding site; chemical shift perturbations upon addition of a peptide from the N-terminal regulatory domain of NDR kinase map the NDR-binding interface to the conserved acidic surface of Mob1, confirming this surface mediates kinase interaction. Heteronuclear multidimensional NMR, chemical shift perturbation mapping Journal of molecular biology High 15001360
2005 The Dbf2-Mob1 kinase complex preferentially phosphorylates substrates containing an RXXS motif (serine over threonine required; arginine at -3 position essential and cannot be substituted by lysine), as determined by peptide library selection and proteome chip screening. Peptide library selection, phosphorylation of optimal peptide variants, proteome microarray BMC biochemistry High 16242037
2006 Human MOB1 activates LATS1 kinase when membrane-targeted (but not when merely co-expressed); activation requires intact phosphorylation sites in LATS1's activation segment and hydrophobic motif, and occurs within minutes of membrane association. A conserved MOB1-binding motif within LATS1 was identified. Co-expression with membrane-targeting constructs, kinase activity assays, site-directed mutagenesis Biochemical and biophysical research communications Medium 16674920
2006 Crystal structure of S. cerevisiae Mob1 (full-length, 2.0 Å) reveals N-terminal structural elements (helix H0, strand S0, strand S-1); H0 mediates intermolecular homodimerization and S0 binds intramolecularly across the Dbf2-binding site. In vivo functional analysis shows Mob1 mutants targeting H0 or its binding site are biologically compromised. X-ray crystallography, in vivo yeast functional analysis Journal of molecular biology High 16934835
2008 MST2 phosphorylates MOB1 at Thr74 in vitro; phosphorylation at Thr74 (but not Thr181) is essential for the formation of a MOB1-MST2-NDR1 scaffold complex and for full NDR1 activation. MOB1-T74A fails to enhance its interaction with NDR1 upon okadaic acid treatment. Knockdown of MOB1 or MST2 abolishes NDR1 activation in cells. In vitro kinase assay, site-directed mutagenesis, co-immunoprecipitation, RNAi knockdown, NDR1 kinase activity assay Oncogene High 18362890
2009 The Dbf2-Mob1 kinase complex directly phosphorylates Cdc14 phosphatase on serine and threonine residues adjacent to its nuclear localization signal (NLS), thereby abrogating NLS activity and promoting Cdc14 relocalization from the nucleolus to the cytoplasm during mitotic exit. In vitro kinase assay, mass spectrometry phosphorylation site mapping, nuclear localization assays The Journal of cell biology High 19221193
2009 Human Mob1 localizes to spindle poles and kinetochores in early mitosis and to the spindle midzone during cytokinesis; chromosomal passenger complex (CPC) disruption abolishes Mob1 recruitment to kinetochores (but not spindle poles), while Plk1 depletion eliminates spindle-pole Mob1. Mob1 depletion delays CPC and MKLP2 relocalization to the spindle midzone in early anaphase. Immunofluorescence, RNAi depletion, live-cell imaging Molecular biology of the cell Medium 19955215
2012 Human Mob1A and Mob1B are required for cytokinetic abscission; RNAi depletion causes abscission failure due to hyper-stabilization of microtubules in the midbody region. Mob1 depletion also increases cell motility and induces prolonged centriole separation in G1; conversely, Mob1A or Mob1B overexpression prevents centrosome splitting. RNAi depletion, microtubule stability assay, live-cell imaging, cytokinesis assay Journal of cell science Medium 22454515
2013 The RING E3 ubiquitin ligase praja2 ubiquitylates and targets MOB1 for proteasomal degradation; this degradation attenuates LATS/NDR kinase activity and the Hippo tumor-suppressor cascade, sustaining glioblastoma growth in vivo. Co-immunoprecipitation, ubiquitylation assay, proteasome inhibition, in vivo tumor growth Nature communications High 23652010
2015 Human MOB1 binds autophosphorylated docking motifs in active MST2, enabling MOB1 phosphorylation by MST2; phosphorylated MOB1 undergoes conformational activation and binds LATS1. Crystal structures of phospho-MST2-MOB1 and phospho-MOB1-LATS1 complexes reveal phosphorylation-dependent binding events. MOB1 mediates LATS1 activation through dynamic scaffolding and allosteric mechanisms. Biochemical binding assays, in vitro kinase assays, X-ray crystallography, functional epistasis Genes & development High 26108669
2015 Mob1a/Mob1b double knockout in mouse liver causes hyperproliferation, hepatocyte dedifferentiation, enhanced epithelial-mesenchymal transition, and development of liver cancers; these phenotypes are strongly dependent on YAP1 and partially on TAZ and TGF-β2/3 signaling, placing MOB1A/B as the critical hub of Hippo signaling upstream of LATS-YAP1/TAZ. Conditional knockout mice, genetic epistasis (Yap1 rescue), drug screening, cell culture assays Proceedings of the National Academy of Sciences of the United States of America High 26699479
2016 Crystal structure of full-length MOB1B reveals an autoinhibited conformation where the N-terminal Switch helix (stabilized by SN strand β-sheet formation) blocks the LATS1-binding surface. MST1/2-mediated phosphorylation of Thr12 and Thr35 structurally relieves autoinhibition via a 'pull-the-string' mechanism, enabling LATS1 binding. X-ray crystallography of full-length MOB1B, complex with LATS1 NTR, biochemical validation Scientific reports High 27335147
2017 MOB1A phosphopeptide-binding specificity is highly complementary to MST1/2 substrate phosphorylation consensus; autophosphorylation of MST1/2 on multiple threonine residues provides several MOB1A-binding sites with varying affinities. Crystal structures of MOB1A with two MST1 phosphopeptides define the phosphopeptide-binding consensus. Phosphopeptide-binding is conserved in six of seven human MOB family members. Proteomics, peptide arrays, biochemical binding assays, X-ray crystallography, interaction proteomics Molecular & cellular proteomics : MCP High 28373298
2017 MOB1 phosphorylation by upstream MST kinases differentially regulates its two protein interaction surfaces: phosphorylation promotes binding to MST via the phosphopeptide-binding surface while modulating LATS/NDR binding at a distinct surface. MOB1A also associates in a phosphorylation-dependent manner with PP6 phosphatase and Rho guanine exchange factor complexes (DOCK6-8) through mechanisms distinct from MST1/2 binding. Biochemical assays, biophysical binding measurements, mutagenesis, structural analysis, interaction proteomics Molecular & cellular proteomics : MCP High 28373297
2017 MOB1/Warts (LATS) binding is essential for tumor suppression, tissue growth control, and development in Drosophila and human cells, while stable MOB1/Hippo (MST) binding is dispensable and MOB1/Trc (NDR) binding alone is insufficient. Crystal structure of the MOB1/NDR2 complex defines key residues for differential binding to Hippo core kinases. Crystal structure, mutagenesis, Drosophila genetic analysis, human cancer cell studies Nature communications High 28947795
2019 Kindlin-2 (focal adhesion molecule) interacts with MOB1 and promotes its degradation by enhancing MOB1-praja2 (E3 ligase) interaction; this inhibits LATS1 phosphorylation and YAP phosphorylation, promoting YAP nuclear translocation. Kindlin-2 depletion activates Hippo/YAP signaling and alleviates renal fibrosis in vivo. Co-immunoprecipitation, ubiquitylation assay, MOB1 degradation assay, mouse knockout with UUO model Cell reports Medium 31825843
2019 PR55α regulatory subunit of PP2A inhibits MOB1-triggered autoactivation of LATS1/2 kinases, thereby promoting YAP activation; PR55α also directly interacts with YAP. This identifies a PP2A-MOB1-LATS1/2 axis controlling YAP in pancreatic cancer cells. Co-immunoprecipitation, kinase activity assay, RNAi knockdown, anchorage-independent growth assay Oncogenesis Medium 31659153
2018 MOB1 stability is regulated by the PTEN-GSK3β axis; GSK3β phosphorylates MOB1 on Ser146, targeting it for ubiquitin-proteasome degradation. PTEN knockdown (via PI3K-AKT) suppresses GSK3β and stabilizes MOB1 protein, promoting neurite outgrowth. In vitro kinase assay, site-directed mutagenesis, ubiquitin-proteasome assay, lentiviral in vivo silencing Cellular and molecular life sciences : CMLS Medium 30069702
2020 The Legionella pneumophila effector kinase LegK7 uses MOB1A as both a substrate and an allosteric activator; MOB1A binding to the LegK7 kinase domain reorients the two kinase lobes into an ATP-binding-compatible closed conformation. The MOB1A N-terminal extension (NTE) serves as a docking site for downstream substrates (YAP1). Crystal structure of the LegK7-MOB1A complex was determined. X-ray crystallography, in vitro kinase assay, mutagenesis of interface residues, cell-based phosphorylation assays Proceedings of the National Academy of Sciences of the United States of America High 32513747
2021 EGFR promotes tyrosine phosphorylation of MOB1 (a core Hippo component) and inactivates LATS1/2 independently of MST1/2, thereby activating YAP/TAZ in head and neck squamous cell carcinoma cells. Phosphorylation assays, kinase inhibition (erlotinib), RNAi and loss-of-function experiments, transcriptomic analysis Communications biology Medium 34725466
2022 MOB1 is acetylated at Lys11 by acetyltransferase CBP and deacetylated by HDAC6; MOB1-K11 acetylation stabilizes MOB1 by reducing its binding to E3 ligase Praja2 and subsequent ubiquitination, and also increases MOB1 phosphorylation and activates LATS1. Oxidative stress promotes MOB1 acetylation by suppressing CBP degradation, independently of MST1/2 kinase activity. Co-immunoprecipitation, ubiquitylation assay, in vitro acetylation assay, site-directed mutagenesis (K11R), in vitro and in vivo tumor assays Nucleic acids research High 35349706
2022 The lncRNA WEE2-AS1 (from CAF-derived exosomes) functions as a scaffold bridging MOB1A and E3 ligase praja2, leading to MOB1A ubiquitin-proteasome degradation, Hippo pathway inhibition, and increased YAP nuclear translocation in colorectal cancer cells. Co-immunoprecipitation, RNA-protein interaction assay, ubiquitylation assay, in vivo tumor model Cell death & disease Medium 36123327
2010 Among all seven human MOB family members, only hMOB1A and hMOB1B interact with both LATS1 and LATS2 in vitro and in vivo; overexpression of hMOB1 in cancer cells activates LATS kinase activity and inhibits proliferation or causes apoptosis, with membrane-targeted hMOB1 producing stronger effects. Co-immunoprecipitation, in vitro binding, kinase activity assay, overexpression and shRNA knockdown in cancer cells International journal of cancer Medium 19739119

Source papers

Stage 0 corpus · 69 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex. Proceedings of the National Academy of Sciences of the United States of America 172 11404483
2015 Dysregulated YAP1/TAZ and TGF-β signaling mediate hepatocarcinogenesis in Mob1a/1b-deficient mice. Proceedings of the National Academy of Sciences of the United States of America 165 26699479
2006 The human tumour suppressor LATS1 is activated by human MOB1 at the membrane. Biochemical and biophysical research communications 162 16674920
1998 MOB1, an essential yeast gene required for completion of mitosis and maintenance of ploidy. Molecular biology of the cell 142 9436989
2015 Structural basis for Mob1-dependent activation of the core Mst-Lats kinase cascade in Hippo signaling. Genes & development 138 26108669
2001 Order of function of the budding-yeast mitotic exit-network proteins Tem1, Cdc15, Mob1, Dbf2, and Cdc5. Current biology : CB 133 11378390
2012 Cancer susceptibility and embryonic lethality in Mob1a/1b double-mutant mice. The Journal of clinical investigation 126 23143302
2013 Proteolysis of MOB1 by the ubiquitin ligase praja2 attenuates Hippo signalling and supports glioblastoma growth. Nature communications 102 23652010
2000 The S. pombe orthologue of the S. cerevisiae mob1 gene is essential and functions in signalling the onset of septum formation. Journal of cell science 100 10769201
2009 Dbf2-Mob1 drives relocalization of protein phosphatase Cdc14 to the cytoplasm during exit from mitosis. The Journal of cell biology 92 19221193
1998 DBF2 protein kinase binds to and acts through the cell cycle-regulated MOB1 protein. Molecular and cellular biology 89 9528782
2020 Human umbilical cord mesenchymal stem cell-derived exosomes act via the miR-1263/Mob1/Hippo signaling pathway to prevent apoptosis in disuse osteoporosis. Biochemical and biophysical research communications 82 32057365
2001 A mammalian homolog of yeast MOB1 is both a member and a putative substrate of striatin family-protein phosphatase 2A complexes. The Journal of biological chemistry 82 11319234
2022 Oxidative stress-CBP axis modulates MOB1 acetylation and activates the Hippo signaling pathway. Nucleic acids research 80 35349706
2005 Substrate specificity analysis of protein kinase complex Dbf2-Mob1 by peptide library and proteome array screening. BMC biochemistry 79 16242037
2000 CCK stimulates mob-1 expression and NF-kappaB activation via protein kinase C and intracellular Ca(2+). American journal of physiology. Cell physiology 77 10666030
2018 Loss of Mob1a/b in mice results in chondrodysplasia due to YAP1/TAZ-TEAD-dependent repression of SOX9. Development (Cambridge, England) 71 29511023
1994 Ras activation of genes: Mob-1 as a model. Proceedings of the National Academy of Sciences of the United States of America 65 7809069
2003 Crystal structure of a human Mob1 protein: toward understanding Mob-regulated cell cycle pathways. Structure (London, England : 1993) 61 12962634
2018 The MST4-MOB4 complex disrupts the MST1-MOB1 complex in the Hippo-YAP pathway and plays a pro-oncogenic role in pancreatic cancer. The Journal of biological chemistry 59 30072378
1999 Cholecystokinin induction of mob-1 chemokine expression in pancreatic acinar cells requires NF-kappaB activation. The American journal of physiology 53 10409110
2021 EGFR Regulates the Hippo pathway by promoting the tyrosine phosphorylation of MOB1. Communications biology 52 34725466
2005 Trypanosoma brucei MOB1 is required for accurate and efficient cytokinesis but not for exit from mitosis. Molecular microbiology 52 15773982
2014 The kinase regulator mob1 acts as a patterning protein for stentor morphogenesis. PLoS biology 51 24823688
2001 Regulation of the localization of Dbf2 and mob1 during cell division of saccharomyces cerevisiae. Genes & genetic systems 51 11434459
2019 Kindlin-2 Inhibits the Hippo Signaling Pathway by Promoting Degradation of MOB1. Cell reports 48 31825843
2008 Threonine 74 of MOB1 is a putative key phosphorylation site by MST2 to form the scaffold to activate nuclear Dbf2-related kinase 1. Oncogene 48 18362890
2022 CAF-derived exosomal WEE2-AS1 facilitates colorectal cancer progression via promoting degradation of MOB1A to inhibit the Hippo pathway. Cell death & disease 45 36123327
2002 Mammalian achaete scute homolog 2 is expressed in the adult sciatic nerve and regulates the expression of Krox24, Mob-1, CXCR4, and p57kip2 in Schwann cells. The Journal of neuroscience : the official journal of the Society for Neuroscience 44 12196582
2017 MOB1 Mediated Phospho-recognition in the Core Mammalian Hippo Pathway. Molecular & cellular proteomics : MCP 42 28373298
2017 MOB1-YAP1/TAZ-NKX2.1 axis controls bronchioalveolar cell differentiation, adhesion and tumour formation. Oncogene 41 28346423
2010 Molecular characterization of human homologs of yeast MOB1. International journal of cancer 39 19739119
2019 PR55α regulatory subunit of PP2A inhibits the MOB1/LATS cascade and activates YAP in pancreatic cancer cells. Oncogenesis 38 31659153
2006 Structural and functional analysis of Saccharomyces cerevisiae Mob1. Journal of molecular biology 35 16934835
2017 Regulation of Protein Interactions by Mps One Binder (MOB1) Phosphorylation. Molecular & cellular proteomics : MCP 34 28373297
2017 Stable MOB1 interaction with Hippo/MST is not essential for development and tissue growth control. Nature communications 34 28947795
2007 Clinical significance of the loss of MATS1 mRNA expression in colorectal cancer. International journal of oncology 34 17611689
2007 Human MOB1 expression in non-small-cell lung cancer. Clinical lung cancer 33 17311693
2004 NMR solution structure of Mob1, a mitotic exit network protein and its interaction with an NDR kinase peptide. Journal of molecular biology 31 15001360
2022 Circular RNA circCCDC85A inhibits breast cancer progression via acting as a miR-550a-5p sponge to enhance MOB1A expression. Breast cancer research : BCR 30 34983617
2016 Structural basis for autoinhibition and its relief of MOB1 in the Hippo pathway. Scientific reports 30 27335147
2012 Mob1: defining cell polarity for proper cell division. Journal of cell science 30 22331360
1997 Mob-1, a Ras target gene, is overexpressed in colorectal cancer. Oncogene 29 9129152
2012 Human Mob1 proteins are required for cytokinesis by controlling microtubule stability. Journal of cell science 27 22454515
2018 PTEN-GSK3β-MOB1 axis controls neurite outgrowth in vitro and in vivo. Cellular and molecular life sciences : CMLS 22 30069702
2020 Lysine demethylase 2 (KDM2B) regulates hippo pathway via MOB1 to promote pancreatic ductal adenocarcinoma (PDAC) progression. Journal of experimental & clinical cancer research : CR 21 31941533
2018 Depletion of MOB1A/B causes intestinal epithelial degeneration by suppressing Wnt activity and activating BMP/TGF-β signaling. Cell death & disease 19 30349003
2006 Alfalfa Mob1-like proteins are involved in cell proliferation and are localized in the cell division plane during cytokinesis. Experimental cell research 19 16460730
2009 Mutual dependence of Mob1 and the chromosomal passenger complex for localization during mitosis. Molecular biology of the cell 18 19955215
1995 Mob-1 expression in IL-2-induced ARDS: regulation by TNF-alpha. The American journal of physiology 17 8572251
2020 The Legionella kinase LegK7 exploits the Hippo pathway scaffold protein MOB1A for allostery and substrate phosphorylation. Proceedings of the National Academy of Sciences of the United States of America 16 32513747
1997 The novel chemokine mob-1: involvement in adult respiratory distress syndrome. Surgery 15 9288136
2020 RNA-binding protein Musashi2 regulates Hippo signaling via SAV1 and MOB1 in pancreatic cancer. Medical oncology (Northwood, London, England) 14 32780197
2019 Loss of Mob1a/b impairs the differentiation of mouse embryonic stem cells into the three germ layer lineages. Experimental & molecular medicine 13 31723125
2022 Tubuloside B, isolated from Cistanche tubulosa, a promising agent against M1 macrophage activation via synergistically targeting Mob1 and ERK1/2. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 11 36076538
2020 Activation of the Legionella pneumophila LegK7 Effector Kinase by the Host MOB1 Protein. Journal of molecular biology 11 33309852
2020 MOB1A regulates glucose deprivation-induced autophagy via IL6-STAT3 pathway in gallbladder carcinoma. American journal of cancer research 10 33294275
2023 Acetylation of MOB1 mediates polyphyllin II-reduced lysosome biogenesis in breast cancer by promoting the cytoplasmic retention of the YAP/TFEB coactivator complex. Phytomedicine : international journal of phytotherapy and phytopharmacology 8 37922793
2015 Growth Inhibition Accompanied by MOB1 Upregulation in Human Acute Lymphoid Leukemia Cells by 3-Deazaneplanocin A. Biochemical genetics 7 26298709
2004 Involvement of M3 cholinergic receptor signal transduction pathway in regulation of the expression of chemokine MOB-1, MCP-1 genes in pancreatic acinar cells. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 6 15315164
2002 MOB-1 and TNF-alpha interact to induce microvascular lung injury. Shock (Augusta, Ga.) 6 12353928
2019 MOB1 regulates thymocyte egress and T-cell survival in mice in a YAP1-independent manner. Genes to cells : devoted to molecular & cellular mechanisms 4 31125466
2021 Characterization of a MOB1 Homolog in the Apicomplexan Parasite Toxoplasma gondii. Biology 3 34943148
2020 MOB1 Inhibits Malignant Progression of Colorectal Cancer by Targeting PAK2. OncoTargets and therapy 3 32943885
2002 Expression of mOb1, a novel atypical 73 amino acid K50-homeodomain protein, during mouse development. Gene expression patterns : GEP 3 12617835
2025 MOB1 deletion in murine mature adipocytes ameliorates obesity and diabetes. Proceedings of the National Academy of Sciences of the United States of America 2 40258148
2009 The mob as tumor suppressor (mats1) gene is required for growth control in developing zebrafish embryos. The International journal of developmental biology 2 19247963
2002 Expression of mOb1, a novel atypical 73 amino acid K50-homeodomain protein, during mouse development. Mechanisms of development 2 14516659
2025 Characterization of temperature-sensitive alleles of the septation initiation network protein Mob1 in Schizosaccharomyces pombe. microPublication biology 1 40385371

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