Affinage

MCRS1

Microspherule protein 1 · UniProt Q96EZ8

Length
462 aa
Mass
51.8 kDa
Annotated
2026-06-10
51 papers in source corpus 24 papers cited in narrative 26 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MCRS1 (MSP58/p78) is a multifunctional FHA-domain protein that partitions between the nucleus/nucleolus and the centrosomal/spindle compartment to coordinate gene expression with cell division (PMID:9654073, PMID:16547491). Its FHA domain serves as a central interaction hub, mediating binding to PTEN, the bHLH factor STRA13, NDRG2, and centrosomal proteins Nde1 and Su48, with FHA-dependent docking also required for centrosomal targeting (PMID:15659546, PMID:15719173, PMID:16547491, PMID:17109818). In the nucleolus MCRS1 partners with RINT-1 at the rDNA promoter to control rRNA synthesis, and its dual nuclear/nucleolar localization signals and importin α binding are required for transcriptional regulation of p21 and ribosomal genes (PMID:25981436, PMID:27530925). As a transcriptional co-regulator it assembles a p53/BRG1 ternary complex to activate p21 and drive p53-dependent senescence, represses Nrf1- and DIPA-dependent transcription, and modulates SIX1/EYA, YY1, and ZEB1 transcriptional programs governing antigen presentation, neural crest/placode fate, and neural commitment (PMID:19187526, PMID:22563078, PMID:32891623, PMID:39545935). At the mitotic and meiotic spindle MCRS1 stabilizes microtubule minus ends and tunes kinetochore-fiber dynamics, with this activity gated by Aurora-A (Ser35/36) and Mps1 phosphorylation that control KIF2A minus-end localization and chromosome alignment (PMID:27192185, PMID:30785839, PMID:36350698). MCRS1 abundance is set by an opposing ubiquitin cycle: the HECT E3 ligase EDD/UBR5 targets it for proteasomal degradation while the deubiquitinase BAP1 stabilizes it to maintain chromosome stability (PMID:23069210, PMID:26300492). MCRS1 also couples amino-acid signaling to growth by maintaining Rheb at lysosomes to enable mTORC1 activation (PMID:25816988), localizes to centriolar satellites to support dynein-dependent cargo transport and ciliogenesis (PMID:27263857), and is essential for epiblast specification in early mouse embryogenesis (PMID:31671403).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1998 High

    Established MCRS1/MSP58 as a nucleolar protein with a defined physical partner, anchoring it to ribosomal/nucleolar biology before any functional role was known.

    Evidence Yeast two-hybrid, Sf9 co-expression and deletion mapping of the MSP58–p120 interaction, with nucleolar imaging in COS-7 cells

    PMID:9654073

    Open questions at the time
    • Functional consequence of p120 binding undefined
    • No link yet between nucleolar localization and a cellular process
  2. 2004 High

    Linked an MCRS1 splice variant to telomere homeostasis, showing a cell-cycle-regulated role beyond static nucleolar residence.

    Evidence Reciprocal binding to LPTS/PinX1, telomerase activity assays and long-term telomere shortening in SMMC-7721 cells

    PMID:15044100

    Open questions at the time
    • Mechanism by which MCRS2 inhibits telomerase not resolved
    • Relationship between the MCRS2 variant and full-length MCRS1 function unclear
  3. 2005 High

    Defined the FHA domain as the protein-interaction module and connected MCRS1 to tumor suppression and transcription, framing it as a transformation-relevant hub.

    Evidence Co-IP and point-mutant analysis mapping PTEN C-terminus to the MSP58 FHA domain (focus-formation rescue), plus FHA-dependent STRA13 binding with reporter and protein-stability assays

    PMID:15659546 PMID:15719173

    Open questions at the time
    • How FHA-domain binding suppresses transformation mechanistically not detailed
    • Phospho-dependence of FHA interactions only partially mapped
  4. 2006 High

    Revealed a centrosomal pool of MCRS1 and additional nuclear transcriptional partners, establishing its dual nuclear/centrosomal lifestyle and FHA-dependent centrosome targeting.

    Evidence Y2H/co-IP/GST pull-down with Nde1, Su48, DIPA and NDRG2; FHA-dependent centrosomal localization imaging; siRNA knockdown causing cell death and mitotic delay

    PMID:16547491 PMID:17014843 PMID:17109818

    Open questions at the time
    • Molecular function of MCRS1 at the centrosome not yet defined
    • Transcriptional targets of DIPA/NDRG2 complexes unidentified
  5. 2009 High

    Extended MCRS1's transcriptional co-regulator role to repression of a specific bZIP factor, generalizing it as a context-dependent modulator of distinct transcription factors.

    Evidence Y2H, GST pull-down, co-IP and luciferase reporter assays mapping the MCRS2–Nrf1 interaction and repression

    PMID:19187526

    Open questions at the time
    • Endogenous Nrf1 target genes affected not identified
    • Whether repression is direct or via recruited corepressors unknown
  6. 2010 High

    Connected MCRS1 to general transcription machinery, showing it associates with RNA Pol II and promotes its promoter recruitment.

    Evidence Co-purification with RNAP II, ChIP showing 5'-gene occupancy and cyclin gene expression effects in Drosophila

    PMID:20679484

    Open questions at the time
    • Whether human MCRS1 directly contacts Pol II not established
    • Mechanism of Pol II recruitment unresolved
  7. 2012 High

    Placed MCRS1 within the p53/p21 senescence axis and identified its degradation pathway, defining both its action on a key tumor suppressor and how its own levels are controlled.

    Evidence ChIP/co-IP of the p53–BRG1–MSP58 ternary complex with p53-dependent senescence assays; in vitro/in vivo EDD(UBR5) binding, ubiquitination and half-life measurements

    PMID:22563078 PMID:23069210

    Open questions at the time
    • Signals controlling EDD-mediated MCRS1 turnover unknown
    • How MCRS1 is recruited to the p21 promoter not fully mapped
  8. 2015 High

    Resolved the trafficking determinants and a deubiquitinase partner of MCRS1, and uncovered an unexpected cytoplasmic role coupling amino acids to mTORC1.

    Evidence NLS/NoLS mutagenesis and importin binding; BAP1 deubiquitination and stability assays; siRNA and Cre/Lox KO showing AA-dependent Rheb retention at lysosomes and mTORC1 control

    PMID:25816988 PMID:25981436 PMID:26300492

    Open questions at the time
    • How MCRS1 physically anchors Rheb at lysosomes not defined
    • Coordination between nuclear and lysosomal pools of MCRS1 unknown
  9. 2016 High

    Established MCRS1 as a microtubule minus-end and centriolar-satellite factor whose mitotic activity is gated by Aurora-A, linking its centrosomal localization to spindle function and ciliogenesis.

    Evidence RINT-1 co-IP/ChIP at rDNA; dynein co-IP, centriolar satellite imaging and zebrafish mcrs1 mutant phenotypes; in vitro Aurora-A phosphorylation of Ser35/36 with mitotic functional assays

    PMID:27192185 PMID:27263857 PMID:27530925

    Open questions at the time
    • Direct biochemical mechanism of minus-end stabilization not defined
    • How Aurora-A phosphorylation alters MCRS1 activity at the molecular level unresolved
  10. 2019 High

    Identified Mps1 as a second mitotic kinase regulating MCRS1 and defined KIF2A as a downstream effector, mechanistically connecting MCRS1 phosphorylation to chromosome alignment.

    Evidence Co-IP and in vitro kinase assays for Mps1–MCRS1, KIF2A localization imaging and chromosome alignment assays; plus Pkmyt1 interaction in gastric cancer cells

    PMID:30785839 PMID:30953699

    Open questions at the time
    • How MCRS1 directs KIF2A specifically to minus ends not detailed
    • Integration of Aurora-A and Mps1 inputs on MCRS1 unknown
  11. 2020 High

    Demonstrated organismal-level requirements for MCRS1 in early embryogenesis and lineage-specific transcriptional control via SIX1.

    Evidence Conditional Mcrs1 mouse KO with epiblast-specific outgrowth failure; Mcrs1–Six1 co-IP, reporter repression and Xenopus morpholino knockdown affecting neural crest/placode genes

    PMID:31671403 PMID:32891623

    Open questions at the time
    • Molecular cause of epiblast-specific defect not identified
    • Whether embryonic phenotypes reflect transcriptional vs spindle functions unresolved
  12. 2022 High

    Provided ultrastructural proof that MCRS1 stabilizes spindle MT minus ends and extended its meiotic role through chromatin and kinase regulation in oocytes.

    Evidence Large-scale electron tomography of HeLa spindles after MCRS1 silencing; siRNA in mouse oocytes linking Mcrs1 to HDAC2/histone marks, CDK1/cyclin B1 and Aurora/Kif2A activities

    PMID:36350698 PMID:36951681

    Open questions at the time
    • Direct binding partners mediating minus-end stabilization not identified
    • Mechanism connecting Mcrs1 to oocyte chromatin modifications unclear
  13. 2024 High

    Expanded MCRS1's transcriptional repertoire to immune evasion and developmental gene control, showing chromatin-level effects on MHC-I and SIX1/EYA programs.

    Evidence CRISPRa screen, YY1 co-IP, ATAC-seq and T-cell/anti-PD-1 models for MHC-I; MCRS1–SIX1 co-IP, reporter repression and EYA nuclear translocation assays (preprint)

    PMID:39545935

    Open questions at the time
    • How MCRS1 increases chromatin accessibility at MHC-I loci not mechanistically defined
    • Whether YY1 and SIX1 effects share a common chromatin mechanism unknown
  14. 2025 Medium

    Positioned MCRS1 as an upstream brake on neural commitment via ZEB1 repression, broadening its developmental gene-regulatory role.

    Evidence Pooled CRISPRi with single-cell multiomics and GRN inference in hiPSC-derived neural progenitors (preprint)

    Open questions at the time
    • MCRS1→ZEB1 repression inferred by GRN modeling without direct biochemical confirmation
    • Preprint, single study not independently replicated

Open questions

Synthesis pass · forward-looking unresolved questions
  • How MCRS1 mechanistically stabilizes microtubule minus ends and whether its nuclear/transcriptional, lysosomal mTORC1, and spindle activities are independent or coordinated remains unresolved.
  • No structural model of MCRS1 at the MT minus end
  • No unifying mechanism linking its distinct subcellular roles
  • Direct effectors of minus-end stabilization unidentified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 5 GO:0008092 cytoskeletal protein binding 3 GO:0060090 molecular adaptor activity 2 GO:0060089 molecular transducer activity 1
Localization
GO:0005634 nucleus 4 GO:0005730 nucleolus 3 GO:0005856 cytoskeleton 3 GO:0005815 microtubule organizing center 2 GO:0005764 lysosome 1
Pathway
R-HSA-1640170 Cell Cycle 4 R-HSA-74160 Gene expression (Transcription) 3 R-HSA-1266738 Developmental Biology 2 R-HSA-162582 Signal Transduction 1 R-HSA-168256 Immune System 1
Partners
BAP1NDE1PTENRINT1SIX1STRA13UBR5YY1
Complex memberships
centriolar satellitescentrosomep53–BRG1 transcriptional complex

Evidence

Reading pass · 26 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 MSP58 (MCRS1) physically interacts with nucleolar protein p120; the interaction requires the coiled-coil domain in the N-terminal region of p120 and the C-terminal region of MSP58, as shown by yeast two-hybrid, recombinant co-expression in Sf9 cells, and deletion mutagenesis. MSP58 localizes to microspherules in the nucleolus, and overexpression causes irregular nucleolar enlargement in COS-7 cells. Yeast two-hybrid, recombinant protein co-expression in insect Sf9 cells, deletion mutagenesis, immunofluorescence European journal of biochemistry High 9654073
2004 MCRS2 (splice variant of MCRS1/p78) interacts with the telomerase-inhibitory protein LPTS/PinX1 in vitro and in vivo, co-localizes with LPTS/PinX1 in cells, inhibits telomerase activity in vitro, and its long-term overexpression progressively shortens telomeres in SMMC-7721 cells. MCRS2 expression is cell-cycle dependent, accumulating in early S phase. Yeast two-hybrid, in vitro binding assay, co-immunoprecipitation, immunofluorescence co-localization, telomerase activity assay, telomere length measurement Biochemical and biophysical research communications High 15044100
2005 The PTEN C-terminal domain physically interacts with the forkhead-associated (FHA) domain of MSP58 (MCRS1), requiring PTEN Thr-366. This interaction suppresses MSP58-driven cellular transformation, as PTEN(T366A) mutant fails to suppress MSP58-induced foci formation, while catalytically inactive PTEN (G129R) retains suppressive activity, demonstrating the C-terminal interaction (not phosphatase activity) is responsible. Co-immunoprecipitation, focus-formation assay in Pten-/- MEFs, point-mutant analysis (T366A, G129R) Proceedings of the National Academy of Sciences of the United States of America High 15659546
2005 MSP58 (MCRS1) interacts with the bHLH transcription factor STRA13 through the basic domain of STRA13 and the FHA domain of MSP58; this interaction is modulated by STRA13 phosphorylation status. Complex formation protects both proteins from proteasome-mediated degradation, extending their half-lives. MSP58 and STRA13 synergistically repress STRA13 promoter-driven transcription and both proteins accumulate during S phase. Yeast two-hybrid, co-immunoprecipitation, phospho-peptide mapping, proteasome inhibitor experiments, luciferase reporter assay, cell-cycle expression analysis Cellular and molecular life sciences : CMLS High 15719173
2006 p78/MCRS1 associates with centrosomal proteins Nde1 and Su48; the FHA domain of p78 is required for these interactions and for centrosomal localization. A fraction of p78 localizes to the centrosome (in addition to the nucleus). The Nde1-p78 interaction is regulated by phosphorylation of Nde1. siRNA knockdown of p78 causes cell death and a modest mitotic delay. Yeast two-hybrid, co-immunoprecipitation, immunofluorescence (centrosome localization), domain mapping, siRNA knockdown with viability and cell-cycle assays Oncogene High 16547491
2006 DIPA (delta-interacting protein A) physically associates with p78/MCRS1/MSP58 as shown by co-immunoprecipitation; the structural regions mediating the interaction were identified. DIPA co-localizes predominantly with p78 in the nucleus, and DIPA acts as a repressor of gene transcription, an activity enhanced by p78. Co-immunoprecipitation, domain mapping, immunofluorescence co-localization, transcriptional reporter assay Experimental and molecular pathology Medium 17014843
2006 NDRG2 interacts with MSP58 (MCRS1); the FHA domain of MSP58 is essential for this interaction. NDRG2 co-localizes with MSP58 in the nucleus of HeLa cells during cell stress. Modulation of NDRG2 levels influences cell cycle progression together with MSP58. Yeast two-hybrid, GST pull-down, co-immunoprecipitation, immunofluorescence co-localization, cell cycle analysis Biochemical and biophysical research communications Medium 17109818
2009 MCRS2 (isoform of MCRS1) directly interacts with the CNC-bZIP transcription factor Nrf1; the interaction domains were mapped to residues 354–447 of Nrf1 and residues 314–475 of MCRS2. MCRS2 co-localizes with Nrf1 in the nucleus and represses Nrf1-mediated transcriptional activation in reporter assays. Yeast two-hybrid, GST pull-down, co-immunoprecipitation, immunofluorescence, luciferase reporter assay BMC cell biology High 19187526
2010 Drosophila MCRS2 (ortholog of human MCRS1/MCRS2) co-purifies with RNA polymerase II complexes, localizes to the 5' ends of genes, and is required for optimal recruitment of RNAP II to promoter regions of cyclin genes and for normal cyclin gene expression levels. Co-purification/co-IP with RNAP II, chromatin immunoprecipitation (ChIP), RNA expression analysis, genetic knockdown Molecular and cellular biology High 20679484
2012 MSP58 (MCRS1) forms a ternary complex with p53 and BRG1 (SWI/SNF subunit) on the p21 promoter to activate p21 transcription. MSP58 also activates the DNA damage response and p53/p21 signaling pathways. MSP58-induced senescence strictly requires functional p53; cells with p53 knockdown or mutant p53 bypass MSP58-induced senescence. Chromatin immunoprecipitation (ChIP), co-immunoprecipitation (ternary complex), shRNA knockdown of p53, ectopic overexpression, senescence assays (β-galactosidase), western blot The Journal of biological chemistry High 22563078
2012 EDD (UBR5), a HECT-domain ubiquitin E3 ligase, directly interacts with MSP58 (MCRS1) in vitro and in vivo. EDD depletion increases MSP58 protein levels and extends its half-life. MSP58 is ubiquitinated and degraded via the ubiquitin-proteasome pathway. Knockdown of either MSP58 or EDD alters cyclin B, D, and E levels and cell cycle progression. Co-immunoprecipitation, in vitro binding assay, proteasome inhibitor (MG132) treatment, ubiquitination assay, siRNA knockdown with western blot and cell cycle analysis Biochimica et biophysica acta High 23069210
2015 MCRS1 is an essential link between Rheb and mTORC1 activation by amino acids (AAs). In an AA-dependent manner, MCRS1 maintains Rheb at lysosome surfaces. MCRS1 depletion (siRNA or Cre/Lox in MEFs) reduces mTORC1 activity, promotes Rheb–TSC2 interaction (rendering Rheb inactive), and delocalizes Rheb from lysosomes to recycling endocytic vesicles. siRNA knockdown, inducible Cre/Lox knockout in MEFs, co-immunoprecipitation, immunofluorescence (lysosomal localization of Rheb), mTORC1 activity assays, amino acid stimulation/deprivation Developmental cell High 25816988
2015 BAP1, a nuclear de-ubiquitinating enzyme, binds MCRS1 and stabilizes it by removing ubiquitin chains (de-ubiquitination), thereby preventing proteasomal degradation of MCRS1. BAP1 contributes to chromosome stability partially through MCRS1. MCRS1 is a component of centrosomal proteins and plays an essential role in spindle assembly. Co-immunoprecipitation, de-ubiquitination assay, BAP1 knockdown/knockout with MCRS1 stability measurement, chromosome instability assays Cancer letters High 26300492
2015 MSP58 (MCRS1) contains two nuclear localization signals (NLS1: residues 32–56; NLS2: residues 113–123) and a bipartite nucleolar localization signal within NLS1 (residues 44–56). MSP58 binds importin α1 and α6, indicating receptor-mediated nuclear import. Both nuclear and nucleolar localization are required for MSP58-mediated transcriptional regulation of p21 and ribosomal RNA genes. Deletion/mutation analysis of NLS/NoLS, yeast two-hybrid, GST pull-down, co-immunoprecipitation with importins, reporter assays, immunofluorescence Journal of biomedical science High 25981436
2016 MCRS1 localizes to centriolar satellites and is required for dynein-dependent cargo transport to the centrosome. MCRS1 knockdown disperses centriolar satellites. MCRS1 plays a positive role in ciliogenesis initiation, possibly through interaction with TTBK2. Zebrafish mcrs1 mutants show brain/eye size reduction due to apoptosis, defective retinal layering, melanosome aggregation defects, and reduced ciliogenesis — phenotypes resembling dynein mutants. Additionally, MCRS1 directly associates with cytoplasmic dynein. siRNA knockdown, immunofluorescence (centriolar satellite localization), zebrafish mcrs1 mutant phenotype analysis, co-immunoprecipitation with dynein components, ciliogenesis assays Scientific reports High 27263857
2016 Aurora-A kinase phosphorylates MCRS1 on Ser35/36 during mitosis. This phosphorylation does not affect MCRS1 localization to chromosomal MTs or K-fiber minus-ends, but regulates MCRS1 activity in mitosis, particularly in tuning kinetochore fiber (K-fiber) minus-end dynamics. In vitro kinase assay, phospho-site mutagenesis, immunofluorescence, siRNA-based functional assays in mitosis Cell cycle (Georgetown, Tex.) Medium 27192185
2016 MCRS1 (MSP58) directly interacts with RINT-1 in vitro and in vivo; both proteins co-localize with UBF in the nucleolus. Overexpression of either MSP58 or RINT-1 decreases rRNA expression and rDNA promoter activity, while co-expression produces a greater decrease. Both MSP58 and RINT-1 associate with the rDNA promoter as shown by ChIP. Yeast two-hybrid, in vitro binding, co-immunoprecipitation, immunofluorescence co-localization, rRNA expression assays, rDNA promoter reporter assay, chromatin immunoprecipitation (ChIP) Biochemical and biophysical research communications High 27530925
2019 Mps1 kinase binds and phosphorylates MCRS1, enabling KIF2A localization to the minus end of spindle microtubules, thereby regulating chromosome alignment. MCRS1 controls the dynamics of the minus end of kinetochore microtubules. Co-immunoprecipitation (Mps1–MCRS1 binding), in vitro kinase assay (phosphorylation), immunofluorescence (KIF2A localization), siRNA/inhibitor-based functional assays (chromosome alignment) Molecular biology of the cell High 30785839
2019 MCRS1 physically interacts with Pkmyt1 (a WEE1-family kinase) in vitro and the two proteins co-localize in the cytoplasm. Overexpression of MCRS1 inhibits growth, invasion and migration of gastric cancer cells and suppresses Pkmyt1 expression, while downregulation of MCRS1 promotes these phenotypes; inhibition of WEE1/Pkmyt1 with MK1775 rescues the MCRS1-knockdown phenotype. Co-immunoprecipitation, immunofluorescence co-localization, overexpression/knockdown cell assays, kinase inhibitor (MK1775) epistasis Cellular signalling Medium 30953699
2020 MCRS1 is essential for epiblast development in early murine embryogenesis. Mcrs1 mutant embryos show normal blastocyst morphology but fail to form an inner cell mass colony in outgrowth assays; trophoblast and primitive endoderm are properly specified, but the epiblast lineage is specifically compromised with severely reduced cell number. Global H4 acetylation and apoptosis are normal in mutant blastocysts. Conditional Mcrs1 knockout mouse, outgrowth assays, lineage marker immunostaining, histone H4 acetylation analysis, apoptosis assay Reproduction (Cambridge, England) High 31671403
2020 Mcrs1 binds to the Six1 transcription factor (confirmed in cultured cells and embryonic ectoderm) and reduces Six1-Eya1 transcriptional activation. Knockdown of Mcrs1 in Xenopus embryos causes expansion of neural plate gene domains, reduction of pre-migratory neural crest genes (foxd3, sox9), and pleiotropic effects on cranial placode genes. Double knockdown and rescue experiments establish a functional interaction between Mcrs1 and Six1 required for otic vesicle development. Co-immunoprecipitation (Mcrs1–Six1 binding), transcriptional reporter assay (Six1-Eya1 activity), antisense morpholino knockdown in Xenopus, rescue experiments, in situ hybridization for lineage markers Developmental biology High 32891623
2022 Mcrs1 localizes to spindle poles and chromosomes of mouse oocytes during meiosis I. Depletion of Mcrs1 alters HDAC2-mediated H4K16ac, H3K4me2, and H3K9me2 chromatin modifications in NSN-type oocytes, reduces CDK1 activity and cyclin B1 accumulation causing G2/M transition delay, and results in abnormal spindle assembly due to reduced Aurora kinase (Aurka, Aurkc) and Kif2A activities. siRNA knockdown in mouse oocytes, immunofluorescence (spindle localization), histone modification immunostaining, CDK1 activity assay, western blot (cyclin B1), Aurora kinase and Kif2A activity assays EMBO reports High 36951681
2022 Silencing MCRS1 increases the proportion of open (versus closed) microtubule minus-end morphologies in metaphase HeLa cell spindles as measured by large-scale electron tomography, demonstrating that MCRS1 stabilizes MT minus ends and modulates their structural heterogeneity. siRNA knockdown of MCRS1, large-scale electron tomography of 3D-reconstructed metaphase spindles in HeLa cells Molecular biology of the cell High 36350698
2024 MCRS1 interacts with the transcription factor YY1 and coordinately increases chromatin accessibility and expression of MHC-I genes, thereby augmenting antigen presentation in pancreatic cancer cells and sensitizing them to T cell-mediated killing and anti-PD-1 therapy. CRISPR activation screen, co-immunoprecipitation (MCRS1–YY1), ATAC-seq/chromatin accessibility assay, MHC-I expression (flow cytometry/western blot), T cell killing assay, in vivo tumor model with anti-PD-1 The Journal of experimental medicine High 39545935
2025 CRISPRi knockdown of MCRS1 in hiPSC-derived neural progenitors drives precocious neural commitment. MCRS1 represses ZEB1 expression, positioning MCRS1 as a brake on premature neurodevelopment. Gene regulatory network inference identified MCRS1 as a key upstream regulator of the ZEB1-mediated neural differentiation axis. Pooled CRISPRi screen with single-cell multiomics (scRNA-seq + ATAC-seq), gene regulatory network (GRN) inference, validation in hiPSC-derived neurons bioRxivpreprint Medium
2024 MCRS1 (Mcrs1) acts as a bona fide SIX1 co-factor that represses SIX1+EYA transcriptional activity in mouse. MCRS1 can translocate EYA to the nucleus indirectly (in contrast to SOBP which does so directly). MCRS1 and SOBP are co-expressed with SIX1 in the oral domain of the mandibular arch. Co-immunoprecipitation (MCRS1–SIX1 binding), transcriptional reporter assay (SIX1+EYA activity), nuclear translocation assay, immunofluorescence co-localization in mouse tissue bioRxivpreprint Medium

Source papers

Stage 0 corpus · 51 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Cellular transformation by the MSP58 oncogene is inhibited by its physical interaction with the PTEN tumor suppressor. Proceedings of the National Academy of Sciences of the United States of America 102 15659546
2015 MCRS1 binds and couples Rheb to amino acid-dependent mTORC1 activation. Developmental cell 63 25816988
2014 The Rift Valley fever accessory proteins NSm and P78/NSm-GN are distinct determinants of virus propagation in vertebrate and invertebrate hosts. Emerging microbes & infections 63 26038497
2014 MCRS1 overexpression, which is specifically inhibited by miR-129*, promotes the epithelial-mesenchymal transition and metastasis in non-small cell lung cancer. Molecular cancer 56 25373388
2000 Structure and polymorphism of the human gene for the interferon-induced p78 protein (MX1): evidence of association with alopecia areata in the Down syndrome region. Human genetics 56 10942113
1998 The 58-kDa microspherule protein (MSP58), a nucleolar protein, interacts with nucleolar protein p120. European journal of biochemistry 55 9654073
2008 Autographa californica multiple nucleopolyhedrovirus nucleocapsid protein BV/ODV-C42 mediates the nuclear entry of P78/83. Journal of virology 51 18287235
2001 TOJ3, a target of the v-Jun transcription factor, encodes a protein with transforming activity related to human microspherule protein 1 (MCRS1). Oncogene 51 11709724
2001 Identification of BV/ODV-C42, an Autographa californica nucleopolyhedrovirus orf101-encoded structural protein detected in infected-cell complexes with ODV-EC27 and p78/83. Journal of virology 44 11711623
2009 Downregulation of MSP58 inhibits growth of human colorectal cancer cells via regulation of the cyclin D1-cyclin-dependent kinase 4-p21 pathway. Cancer science 43 19549253
2015 Stabilization of MCRS1 by BAP1 prevents chromosome instability in renal cell carcinoma. Cancer letters 40 26300492
2012 Analysis of 20 genes at chromosome band 12q13: RACGAP1 and MCRS1 overexpression in nonsmall-cell lung cancer. Genes, chromosomes & cancer 40 23225332
2012 58-kDa microspherule protein (MSP58) is novel Brahma-related gene 1 (BRG1)-associated protein that modulates p53/p21 senescence pathway. The Journal of biological chemistry 34 22563078
2005 Association, mutual stabilization, and transcriptional activity of the STRA13 and MSP58 proteins. Cellular and molecular life sciences : CMLS 34 15719173
2004 Human MCRS2, a cell-cycle-dependent protein, associates with LPTS/PinX1 and reduces the telomere length. Biochemical and biophysical research communications 33 15044100
2006 p78/MCRS1 forms a complex with centrosomal protein Nde1 and is essential for cell viability. Oncogene 32 16547491
2006 The physical and functional interaction of NDRG2 with MSP58 in cells. Biochemical and biophysical research communications 32 17109818
2006 DIPA, which can localize to the centrosome, associates with p78/MCRS1/MSP58 and acts as a repressor of gene transcription. Experimental and molecular pathology 29 17014843
2009 RNAi-mediated inhibition of MSP58 decreases tumour growth, migration and invasion in a human glioma cell line. Journal of cellular and molecular medicine 22 18798870
2009 MCRS2 represses the transactivation activities of Nrf1. BMC cell biology 22 19187526
2019 Effects of MCRS1 on proliferation, migration, invasion, and epithelial mesenchymal transition of gastric cancer cells by interacting with Pkmyt1 protein kinase. Cellular signalling 21 30953699
2012 The novel interaction between microspherule protein Msp58 and ubiquitin E3 ligase EDD regulates cell cycle progression. Biochimica et biophysica acta 21 23069210
2020 MCRS1 is essential for epiblast development during early mouse embryogenesis. Reproduction (Cambridge, England) 20 31671403
2015 The candidate oncogene (MCRS1) promotes the growth of human lung cancer cells via the miR-155-Rb1 pathway. Journal of experimental & clinical cancer research : CR 20 26467212
2010 Drosophila MCRS2 associates with RNA polymerase II complexes to regulate transcription. Molecular and cellular biology 20 20679484
2019 miR-186 modulates hepatocellular carcinoma cell proliferation and mobility via targeting MCRS1-mediated Wnt/β-catenin signaling. Journal of cellular physiology 17 31140612
2013 Expression of MSP58 in hepatocellular carcinoma. Medical oncology (Northwood, London, England) 14 23519485
2020 Mcrs1 interacts with Six1 to influence early craniofacial and otic development. Developmental biology 13 32891623
2016 MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment. Scientific reports 12 27263857
2015 Identification and characterization of nuclear and nucleolar localization signals in 58-kDa microspherule protein (MSP58). Journal of biomedical science 12 25981436
2012 MSP58 knockdown inhibits the proliferation of esophageal squamous cell carcinoma in vitro and in vivo. Asian Pacific journal of cancer prevention : APJCP 11 22994740
2023 Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis. EMBO reports 10 36951681
2019 Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment. Molecular biology of the cell 9 30785839
2012 Downregulation of MSP58 suppresses cell proliferation in neuroblastoma cell lines. Neuroreport 9 22975844
1998 The late expression factors 8 and 9 and possibly the phosphoprotein p78/83 of Autographa californica multicapsid nucleopolyhedrovirus are components of the virus-induced RNA polymerase. Intervirology 8 9705563
2024 MCRS1 sensitizes T cell-dependent immunotherapy by augmenting MHC-I expression in solid tumors. The Journal of experimental medicine 7 39545935
2011 Mapping signals that are important for nuclear and nucleolar localization in MCRS2. Molecules and cells 7 21533551
1989 Two PstI DNA polymorphisms adjacent to the human gene for the interferon-induced p78 protein (MX1 gene). Nucleic acids research 7 2798118
2016 Expression of 58-kD Microspherule Protein (MSP58) is Highly Correlated with PET Imaging of Tumor Malignancy and Cell Proliferation in Glioma Patients. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 5 26849376
2016 Aurora-A regulates MCRS1 function during mitosis. Cell cycle (Georgetown, Tex.) 5 27192185
2016 Increased Expression of 58-kDa Microspherule Protein (MSP58) in Human Gastric Cancer Promotes Cell Proliferation and Correlates with Poor Patient Survival. Clinical laboratory 5 27468560
2017 Knockdown of MSP58 inhibits the proliferation and metastasis in human renal cell carcinoma cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 4 28448870
2016 Expression of MCRS1 and MCRS2 and their correlation with serum carcinoembryonic antigen in colorectal cancer. Experimental and therapeutic medicine 4 27446248
2016 RINT-1 interacts with MSP58 within nucleoli and plays a role in ribosomal gene transcription. Biochemical and biophysical research communications 4 27530925
2022 Mcrs1 is required for branchial arch and cranial cartilage development. Developmental biology 3 35697116
2025 Isolation and characterization of a novel lytic bacteriophage vB_EcoS_P78 against multidrug-resistant Escherichia coli. Virus research 2 41173327
2022 MCRS1 Expression Regulates Tumor Activity and Affects Survival Probability of Patients with Gastric Cancer. Diagnostics (Basel, Switzerland) 2 35741311
2022 MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles. Molecular biology of the cell 2 36350698
2026 Integrated multi-omics identifies MCRS1 as a causal hub linking aging, metabolic syndrome, and breast cancer progression. International journal of surgery (London, England) 0 41556158
2025 MCRS1 is associated with immunosuppressive microenvironments in pan-cancer and promotes hepatocellular carcinoma malignant phenotypes. Translational cancer research 0 41378029
2020 Analysis of P78: A Novel Cytoplasmic Membrane-Associated Protein Encoded on Chromosome 19q13.3 in Glioma Specimens. Journal of molecular neuroscience : MN 0 32367506

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