Affinage

LMO4

LIM domain transcription factor LMO4 · UniProt P61968

Length
165 aa
Mass
18.0 kDa
Annotated
2026-06-10
100 papers in source corpus 53 papers cited in narrative 52 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

LMO4 is a nuclear LIM-only adaptor protein that nucleates context-dependent multi-protein transcriptional complexes through its tandem LIM domains, governing cell fate, proliferation, and differentiation across neural, epithelial, and immune lineages (PMID:11751867, PMID:16899735, PMID:19323994). Atomic-resolution structures establish its central architecture: LDB1/NLI binds across both LIM domains in an extended conformation, contributing a third strand to a LIM1 beta-hairpin (PMID:12727888, PMID:15343268), while context-specific partners including CtIP/RBBP8 and DEAF1 engage a common binding face on LMO4 that overlaps the LDB1 site, making partner engagement mutually competitive (PMID:23353824, PMID:25310299). The second LIM domain is required for CtIP and BRCA1 association, within which LMO4 forms a trimeric complex that represses BRCA1-mediated transcription (PMID:11751867, PMID:12925972). Through these modular complexes LMO4 directs binary cell-fate choices—collaborating with SCL, Gata2 and NLI to assemble a V2b interneuron enhancer complex and with NGN2/LDB1 to activate cortical neuronal differentiation genes (PMID:19323994, PMID:21652654)—and acts as a ligand-dependent PPARγ cofactor and a Snail2 cofactor driving neural crest EMT (PMID:19020036, PMID:22119055, PMID:23407937). LMO4 regulates target genes in part by displacing HDAC2 from promoters, as shown for BMP7 (PMID:17452977), and controls calcium-dependent transcription downstream of NMDA receptors and voltage-sensitive calcium channels via CaMKIV/MAPK/CREB (PMID:16899735). It positively regulates RyR2 to enable calcium-induced calcium release and synaptic plasticity (PMID:22442089) and is required for neural tube closure, vestibular morphogenesis, and restriction of cochlear sensory organ formation, in the latter through reciprocal antagonism with Lmx1a (PMID:14966285, PMID:15691703, PMID:19913004, PMID:25057208, PMID:29769265). In CD8+ T cells LMO4 binds JAK1 directly to potentiate IL-21–STAT3 signaling and stem-like memory formation (PMID:39117617), and the same LMO4/STAT3 axis confers otoprotection in cochlear hair cells (PMID:33411315). LMO4 is itself targeted for proteasomal degradation—by peroxynitrite-mediated tyrosine nitration of its LIM domains during cisplatin ototoxicity (PMID:22493493) and by K48-linked ubiquitination at K29/K67 driven by the Salmonella effector SsPH2 (PMID:40693870). LMO4 overexpression is oncogenic in breast and oral cancers, promoting proliferation, invasion, and substrate degradation (PMID:15897450, PMID:41213908).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 1998 Medium

    Established LMO4's foundational molecular partnership by identifying it as a binding protein for the nuclear LIM interactor LDB1/NLI and for DEAF-1, defining a new LIM-interaction module.

    Evidence Yeast two-hybrid and expression-library screens with domain mapping

    PMID:9736723 PMID:9840944 PMID:9860983

    Open questions at the time
    • No structural definition of the interaction interface yet
    • Functional transcriptional consequence not established
    • Displacement of LMO4 by LMO1/LMO2 inferred, not directly demonstrated
  2. 2001 High

    Showed LMO4 assembles a defined trimeric complex with BRCA1 and CtIP and represses BRCA1-mediated transcription, giving LMO4 its first concrete role as a transcriptional regulator and tumor-relevant adaptor.

    Evidence Yeast two-hybrid, reciprocal Co-IP, and transcription reporter assays in yeast and mammalian cells; mammary epithelial overexpression

    PMID:11734645 PMID:11751867

    Open questions at the time
    • Promoters/genes directly regulated by the complex not mapped
    • Domain requirements for each partner not yet resolved
  3. 2003 High

    Resolved the molecular basis of LMO4 partner binding by solving the LMO4:LDB1-LID structure and mapping that the second LIM domain is required for CtIP/BRCA1 binding, converting a list of interactors into a structural model.

    Evidence NMR solution structure of LMO4:ldb1-LID; truncation-mutant binding assays; nuclear Co-IP in carcinoma cells; mammalian two-hybrid with HEN1

    PMID:12727888 PMID:12771919 PMID:12877980 PMID:12878195 PMID:12925972

    Open questions at the time
    • Structure covered only LIM1:LID, not the full tandem interface
    • How distinct partners share or compete for surfaces unresolved
  4. 2004 High

    Defined the complete tandem LIM:LDB1 interface by crystallography and established LMO4's in vivo developmental requirement, linking the structural module to neural tube closure and a functional DEAF1 partnership.

    Evidence High-resolution X-ray crystal structure with mutagenic validation; Lmo4-null and Deaf-1-null mice with histology, BrdU, and TUNEL; phenotypic epistasis

    PMID:14966285 PMID:14966286 PMID:15343268 PMID:15691703

    Open questions at the time
    • Transcriptional targets driving the neural tube phenotype not identified
    • Whether DEAF1 and LDB1 compete for LMO4 not yet tested structurally
  5. 2005 High

    Extended LMO4 beyond the nucleus by showing it associates with the gp130/JAK1 receptor complex and potentiates STAT3, and demonstrated its oncogenic potential in vivo, establishing roles in both cytokine signaling and breast tumorigenesis.

    Evidence Yeast two-hybrid, in vitro binding, Co-IP, siRNA, reporter assays; MMTV-Lmo4 transgenic mice, siRNA, invasion/motility assays; conditional mammary knockout with BrdU

    PMID:15677447 PMID:15856027 PMID:15897450

    Open questions at the time
    • Direct vs. scaffolded nature of the gp130/JAK1 association unresolved at this stage
    • Mechanism coupling cytoplasmic LMO4 to nuclear transcription unclear
  6. 2006 High

    Connected LMO4 to activity-dependent and growth-factor signaling, showing it mediates calcium-dependent transcription via CaMKIV/MAPK/CREB and integrates TGFβ/Smad and LKB1/GATA inputs.

    Evidence Calcium-stimulated reporter assays, pharmacological inhibitors, Co-IP, conditional cortical knockout with barrel-field histology; Smad Co-IP/ChIP; LKB1 complex Co-IP and p21 reporter

    PMID:16331278 PMID:16580634 PMID:16899735

    Open questions at the time
    • Direct DNA contacts of LMO4-containing complexes not defined
    • How calcium signaling alters LMO4 complex composition unknown
  7. 2007 High

    Defined a chromatin mechanism for LMO4-mediated gene activation—displacement of HDAC2 from target promoters—and tied LMO4 to ATP/HuD-dependent post-transcriptional regulation in neuronal survival.

    Evidence ChIP of LMO4/Clim2/HDAC2 at BMP7 promoter, microarray, reporter, siRNA; 3'UTR reporter and RNA-binding-protein assays for HuD/CUGBP1

    PMID:17418808 PMID:17452977 PMID:17524392

    Open questions at the time
    • Whether HDAC displacement is a general LMO4 mechanism beyond BMP7 untested
    • Direct vs. indirect recruitment to promoters not fully resolved
  8. 2008 High

    Established LMO4 as a cell-fate and morphogenesis regulator across tissues—genetically with GRHL3 in epidermal differentiation/neural tube closure and as an essential ligand-dependent PPARγ cofactor in neuronal ischemia protection.

    Evidence Compound Grhl3/Lmo4 and Get-1/Lmo4 knockouts with EM, phospho-ERK staining, scratch assays; PPARγ Co-IP, mammalian two-hybrid, conditional KO, focal ischemia, SOD2 reporter, pharmacological rescue

    PMID:16949565 PMID:18619436 PMID:19020036

    Open questions at the time
    • Direct biochemical interface for the GRHL3 interaction not structurally mapped
    • How LMO4 selects PPARγ versus other partners in vivo unknown
  9. 2009 High

    Demonstrated that LMO4 nucleates distinct LIM-complexes to make binary cell-fate decisions, switching V2 spinal interneurons toward an inhibitory fate via an SCL/Gata2/NLI complex, and linked LMO4 to cell-cycle-coupled proliferation in cancer.

    Evidence Conditional and compound (LMO4;SCL) mutants, enhancer reporters, Co-IP; ErbB2/PI3K profiling, siRNA, FACS cell-cycle analysis with cullin-3/cyclin readouts

    PMID:19323994 PMID:19648968

    Open questions at the time
    • How partner availability determines which complex LMO4 forms in a given cell unresolved
    • Mechanism linking LMO4 to cullin-3 oscillation not defined
  10. 2011 High

    Generalized LMO4's co-activator logic by showing it bridges NLI/LDB1 to the bHLH factor NGN2 for cortical neuronal differentiation and serves as an essential Snail/Slug cofactor for neural crest induction.

    Evidence Co-IP of LMO4-NLI-NGN2 trimer, ChIP at E-box enhancers, Lmo4-null embryos, in utero electroporation migration assay; Xenopus morpholino knockdown, misexpression, Slug/Snail binding; ERα/Alk ChIP

    PMID:21652654 PMID:21976498 PMID:22119055

    Open questions at the time
    • Whether the same LMO4 surface engages bHLH partners as engages LDB1 not structurally shown here
    • Direct vs. complex-mediated contact with Snail2 unresolved
  11. 2012 High

    Defined LMO4 control of neuronal calcium handling (RyR2/CICR) and synaptic plasticity, mapped its regulation of DEAF1 nuclear export, and identified the first post-translational off-switch—tyrosine nitration during cisplatin ototoxicity driving its loss.

    Evidence Conditional forebrain KO with electrophysiology, two-photon imaging, single-cell rescue, Morris water maze; NES localization assays; MALDI-TOF identification of LMO4 nitration with reciprocal IP and co-localization; p53 Co-IP

    PMID:22442089 PMID:22493493 PMID:22723967 PMID:22906635

    Open questions at the time
    • How RyR2 transcription is mechanistically activated by LMO4 not detailed
    • Which nitrated tyrosines drive degradation and the responsible E3 not identified at this stage
  12. 2013 High

    Resolved the competitive-binding principle underlying LMO4 partner selection (CtIP and LDB1 share one face) and confirmed LMO4 as an essential cofactor for Snail2-driven EMT and cadherin repression.

    Evidence NMR structure of LMO4:CtIP/RBBP8 with competition assays; siRNA/shRNA knockdown, invasion and chick neural crest delamination assays, cadherin analysis; HBXIP/Sp1 promoter activation by ChIP/EMSA

    PMID:23291272 PMID:23353824 PMID:23407937

    Open questions at the time
    • In vivo determinants of which competing partner wins not established
    • Direct LMO4 contact with Snail2 versus scaffolded interaction unresolved
  13. 2014 High

    Broadened LMO4's competitive-surface model to DEAF1 (disorder-to-order on a shared face) and detailed its tissue-specific roles in retinal interneuron specification, cochlear sensory restriction, and hypothalamic control of Ca2+ channels, excitability, feeding, and insulin signaling.

    Evidence NMR of LMO4-LIM2:DEAF1 with mutagenesis; conditional KOs in retina (ERG), cochlea (ectopic organ of Corti), PVH and glutamatergic neurons with electrophysiology, RT-PCR of Ca2+ channels, DREADD and PTP1B-inhibitor rescue

    PMID:20949055 PMID:24381275 PMID:24937445 PMID:25057208 PMID:25310299

    Open questions at the time
    • Direct transcriptional targets in each tissue largely unmapped
    • How a single competitive surface accommodates so many tissue partners unresolved
  14. 2015 High

    Established the LMO4/STAT3 axis as an otoprotective survival pathway, showing LMO4 deficiency compromises STAT3 phosphorylation and increases hair-cell apoptosis after cisplatin, and defined behavioral roles in cue-reward learning.

    Evidence Hair-cell-specific conditional KO with ABR and phospho-STAT3/caspase-3 staining; BLA-specific RNAi with conditioned-reinforcement and D2 electrophysiology; cisplatin cochlear gene array with Trolox rescue

    PMID:25501662 PMID:26134647 PMID:33411315

    Open questions at the time
    • Mechanism by which LMO4 supports STAT3 phosphorylation in cochlea not fully defined
    • The cochlear array link to LMO4 is correlative (Low confidence)
  15. 2018 High

    Defined reciprocal genetic antagonism between Lmo4 and Lmx1a as the switch patterning inner-ear sensory versus non-sensory fate, refining LMO4's role as a negative regulator of ectopic sensory organ formation.

    Evidence Conditional and compound Lmx1a/Lmo4 knockouts with histology and otic-marker in situ hybridization

    PMID:29769265

    Open questions at the time
    • Molecular mechanism by which each gene represses the other unknown
    • Whether the antagonism is transcriptional or post-translational unresolved
  16. 2019 Medium

    Showed how Lmo4 transcription is itself epigenetically initiated—via Yeats4/Dot1l/RNA Pol II recruitment to its H3K27ac-marked promoter—and placed LMO4 in innate lymphoid cell lineage differentiation.

    Evidence Conditional Yeats4 KO, ChIP of Yeats4/Dot1l/RNA Pol II at the Lmo4 promoter, H3K27ac recognition assays, ILC phenotyping

    PMID:31434684

    Open questions at the time
    • Direct LMO4 targets driving ILC differentiation not identified
    • Single-lab finding without orthogonal upstream confirmation
  17. 2024 High

    Defined a direct cytoplasmic signaling mechanism: LMO4 binds JAK1 to potentiate IL-21–STAT3 signaling and drive stem-like CD8+ T-cell memory, with STAT3 deletion nullifying the effect—establishing LMO4 as a STAT3-pathway amplifier with antitumor immune relevance.

    Evidence CRISPR-activation screen, overexpression, LMO4-JAK1 Co-IP, CRISPR/Cas9 Stat3 deletion epistasis, syngeneic and xenograft tumor models, transcriptomics

    PMID:39117617

    Open questions at the time
    • Whether LMO4-JAK1 binding is direct via LIM domains not structurally mapped
    • Generality of the JAK1/STAT3 axis beyond CD8+ T cells untested here
  18. 2025 High

    Identified the degradative control points and an oncogenic substrate of LMO4: the Salmonella effector SsPH2 drives K48-linked ubiquitination at K29/K67 in the LIM domains to suppress gp130/STAT3, while LMO4 itself promotes proteasomal degradation of the tumor suppressor RAB17 in oral cancer.

    Evidence Yeast two-hybrid, GST pull-down, in vitro/in vivo ubiquitination with lysine mutagenesis, proteasome-inhibitor rescue; siRNA/overexpression with ubiquitin-proteasome assay and OSCC xenografts

    PMID:40693870 PMID:41213908

    Open questions at the time
    • The endogenous host E3 ligase regulating LMO4 turnover not identified
    • Mechanism by which LMO4 directs RAB17 degradation (adaptor vs. ligase recruitment) unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How LMO4 selects among its many mutually competitive partners in a given cell—and the upstream signals and host E3 ligases that tune its abundance to switch complex composition—remains the central open question.
  • No cell-type-resolved map of which competing partner occupies LMO4 in vivo
  • Endogenous regulators of LMO4 stability beyond pathogen effectors and nitration unknown
  • No structure of LMO4 bound to its bHLH (NGN2/SCL) or JAK1 partners

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 6 GO:0140110 transcription regulator activity 6 GO:0098772 molecular function regulator activity 3
Localization
GO:0005634 nucleus 4 GO:0005829 cytosol 2
Pathway
R-HSA-1266738 Developmental Biology 6 R-HSA-162582 Signal Transduction 5 R-HSA-74160 Gene expression (Transcription) 5 R-HSA-392499 Metabolism of proteins 3 R-HSA-168256 Immune System 2
Partners
BRCA1CREBCTIP/RBBP8DEAF1JAK1LDB1NGN2PPARG
Complex memberships
LMO4-BRCA1-CtIP complexLMO4-LDB1/NLI complexLMO4-NLI-NGN2 complexLMO4-SCL-Gata2-NLI V2b complex

Evidence

Reading pass · 52 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 LMO4 was identified as a binding partner of LDB1/NLI1 via yeast two-hybrid screening; nuclear retention of LMO proteins is enhanced by the nuclear LIM interactor (NLI/LDB1). Yeast two-hybrid screen, in situ hybridization Proceedings of the National Academy of Sciences of the United States of America Medium 9736723
1998 LMO4 interacts strongly with CLIM/LDB coregulators via its LIM domain, and interacts with mouse DEAF-1 (DEFORMED EPIDERMAL AUTOREGULATORY FACTOR 1) through a proline-rich C-terminal domain of DEAF-1, defining a novel LIM-interacting domain distinct from HLH and GATA domains. Expression library screen using CLIM LIM-interaction domain as bait; yeast two-hybrid; domain mapping Proceedings of the National Academy of Sciences of the United States of America Medium 9860983
1998 LMO4 interacts with LDB1/NLI1, and enforced LMO1/LMO2 expression may displace LMO4 from its complex with LDB1 in T cell development. Yeast two-hybrid identification of LMO4 via LDB1 interaction Oncogene Low 9840944
2001 LMO4 interacts with the cofactor CtIP and with the BRCA1 tumor suppressor (via BRCA1's C-terminal BRCT domains); a stable trimeric complex of LMO4, BRCA1, and CtIP was demonstrated in vivo, and LDB1 also participates. LMO4 represses BRCA1-mediated transcriptional activation in both yeast and mammalian cells. Yeast two-hybrid screen, co-immunoprecipitation (in vivo complex), functional transcription reporter assays in yeast and mammalian cells The Journal of biological chemistry High 11751867
2001 Forced expression of LMO4 inhibited differentiation of mammary epithelial cells in vitro, consistent with a role in maintaining proliferation. Forced expression/overexpression in mammary epithelial cells, differentiation assays Proceedings of the National Academy of Sciences of the United States of America Medium 11734645
2003 NMR solution structures of LMO2-LIM1:ldb1-LID and LMO4-LIM1:ldb1-LID complexes show that ldb1-LID binds to the N-terminal LIM domain of LMO4 in an extended conformation, contributing a third strand to a beta-hairpin in LIM1 domains, providing the first molecular definition of LIM-mediated protein-protein interactions. NMR solution structure determination (PDB: 1M3V and 1J2O) The EMBO journal High 12727888
2003 LMO4 immunoprecipitates with LDB1 in the nuclear fraction of oral carcinoma cells, confirming formation of an LMO4-LDB1 transcription complex in vivo. Co-immunoprecipitation from nuclear fractions of carcinoma cells British journal of cancer Low 12771919
2003 LMO4 modulates the transcriptional activity of the bHLH protein HEN1: LMO4 interacts with HEN1 (by mammalian two-hybrid), and LMO4 but not LMO2 augments HEN1-mediated repression of E47 transcription. Overexpression of LMO4 in hippocampal precursor cells prevents HEN1-induced neurite extension. Mammalian two-hybrid assay, reporter gene assays, overexpression in hippocampal precursor cells Biochemical and biophysical research communications Medium 12878195
2003 Overexpression of LMO4 (sense orientation) reduces neurite number and length in SH-SY5Y neuroblastoma cells, while antisense LMO4 promotes neuritogenesis; LMO4-dependent neuritic effects correlate with altered phosphorylation of cytoskeletal proteins. Stable transfection of sense/antisense LMO4 cDNA, morphometric analysis, phosphorylation analysis Brain research. Molecular brain research Medium 12877980
2003 A truncating somatic mutation in LMO4 (frameshift at exon 2) generates a LIM1-only mutant that retains LDB1 binding but loses CtIP and BRCA1 association, demonstrating the second LIM domain is required for CtIP/BRCA1 interaction. Mutation analysis, binding assays comparing wild-type vs. mutant LMO4 International journal of cancer Medium 12925972
2004 X-ray crystal structure of LMO4 in complex with LDB1-LID (the FLINC4 complex) at high resolution reveals a highly modular structure with Ldb1-LID binding in an extended manner across both LIM domains of LMO4, with extensive hydrophobic and electrostatic interactions. Mutagenic screening of Ldb1-LID (yeast two-hybrid and competition ELISA) identified key interface residues. X-ray crystallography (high-resolution crystal structure), mutagenic screen assessed by yeast two-hybrid and competition ELISA The EMBO journal High 15343268
2004 Lmo4-null mice die perinatally and show severe neural tube defect (exencephaly/anencephaly); conditional null demonstrates LMO4 is required for proper closure of the anterior neural tube, with loss causing abnormal cell proliferation and elevated apoptosis in the neuroepithelium. LMO4 is expressed in neuroepithelial cells and Schwann cell progenitors. Gene targeting (null mutation), histology, BrdU proliferation assay, TUNEL apoptosis assay Molecular and cellular biology / Molecular and cellular neurosciences High 14966285 15691703
2004 The transcription factor Deaf-1/NUDR is a binding partner of LMO4 in vivo; Deaf-1 knockout mice share overlapping phenotypes with Lmo4 knockouts (exencephaly, vertebral homeotic transformations), indicating they act in a functional complex. Gene targeting of both Lmo4 and Deaf-1 in mice, phenotypic comparison (genetic epistasis) Molecular and cellular biology High 14966286
2005 LMO4 overexpression in the mouse mammary gland (MMTV-Lmo4 transgene) elicits hyperplasia and mammary intraepithelial neoplasia; siRNA knockdown of LMO4 reduces proliferation of breast cancer cells and increases differentiation of mammary epithelial cells; LMO4 overexpression in MCF10A cells promotes cell motility and invasion of extracellular matrix. Transgenic mouse model (MMTV-Lmo4), siRNA knockdown, Matrigel invasion assay, cell motility assay Proceedings of the National Academy of Sciences of the United States of America High 15897450
2005 Conditional deletion of Lmo4 in mammary epithelium during pregnancy impairs lobuloalveolar development with a 2-fold reduction in BrdU-positive proliferating cells, demonstrating LMO4 is a positive regulator of alveolar epithelial proliferation. Conditional knockout (Cre-lox), BrdU incorporation, mammary gland histology Oncogene High 15856027
2006 LMO4 mediates calcium-dependent transcription in cortical neurons: calcium influx via VSCCs and NMDA receptors activates LMO4-mediated transactivation via CaM kinase IV and MAP kinase. LMO4 forms a complex with CREB, CLIM1, and CLIM2 (by co-immunoprecipitation). Conditional cortical deletion of Lmo4 disrupts barrel field segregation of thalamocortical afferents in somatosensory cortex. Luciferase reporter assay (calcium stimulation), pharmacological inhibitors, co-immunoprecipitation, conditional Cre-lox knockout, barrel field histology The Journal of neuroscience High 16899735
2006 LMO4 interacts with the MH1 and linker domains of receptor-mediated Smad proteins and associates with the endogenous TGFβ-responsive PAI-1 gene promoter in a TGFβ-dependent manner; LMO4 modulates TGFβ signaling in epithelial cells and potentiates growth-inhibitory effects of TGFβ. Co-immunoprecipitation (LMO4-Smad interaction), chromatin immunoprecipitation (LMO4 at PAI-1 promoter), TGFβ-responsive reporter gene assays Oncogene Medium 16331278
2006 Get-1/Grhl3 and LMO4 interact functionally (genetically) in epidermal terminal differentiation: double knockout of Get-1 and LMO4 causes more severe stratum corneum defects than Get-1 single knockout alone. Compound knockout mouse model (Get-1−/−; LMO4−/−), epidermal barrier assays, histology Developmental biology Medium 16949565
2007 LMO4 regulates expression of BMP7 by a mechanism involving displacement of HDAC2 from the BMP7 promoter: LMO4 and its cofactor Clim2 are recruited to the BMP7 promoter (ChIP), HDAC2 recruitment is inhibited by LMO4 upregulation, and HDAC2 knockdown upregulates the promoter. Inhibition of BMP7 partially blocks LMO4-mediated effects on apoptosis. DNA microarray, promoter-reporter assay, chromatin immunoprecipitation (LMO4, Clim2, HDAC2 at BMP7 promoter), siRNA knockdown Oncogene High 17452977
2007 LMO4 is a downstream effector of ATP signaling that promotes neuron survival from hypoxia; extracellular ATP stabilizes LMO4 mRNA via the ARE1 element in the 3'UTR by increasing HuD protein binding, while CUGBP1 binds sequences flanking ARE2 and is destabilized by ATP. ERK and CREB activation by ATP increases LMO4 promoter activity and LMO4 protein stability. siRNA knockdown, luciferase 3'UTR reporter, RNA-binding protein assays (HuD/CUGBP1), primary LMO4-null neurons Experimental cell research / Biochemical and biophysical research communications Medium 17418808 17524392
2008 GRHL3 and LMO4 interact biochemically and genetically; compound Grhl3/Lmo4 null mice show fully penetrant exencephaly, spina bifida, and eyes-open-at-birth phenotype not seen in individual nulls. ERK1/2 phosphorylation is lost in the epidermis of double-null embryos, actin cable formation is absent at eyelid margins, and keratinocytes from double-null mice fail to heal in scratch assays. Compound knockout mouse model, biochemical interaction assay, electron microscopy, phospho-ERK immunostaining, in vitro scratch assay Developmental biology High 18619436
2008 LMO4 is required for vestibular morphogenesis: Lmo4-null mice lack all three semicircular canals and anterior/posterior cristae; canal outpouches fail to form and cell proliferation is reduced in the dorsolateral otocyst. LMO4 controls expression of Bmp4, Fgf10, Msx1, Isl1, Gata3, and Dlx5 in the dorsolateral otocyst. Gene targeting (null mutation), BrdU proliferation assay, in situ hybridization for otic markers Developmental biology High 19913004
2005 LMO4 associates with gp130 and JAK1 in mammalian cells; it also interacts with SHP2 and SOCS3, and exists in the gp130 IL-6 receptor complex. LMO4 overexpression enhances STAT3 transcriptional activity and target gene expression, while LMO4 siRNA knockdown decreases STAT3 activity. LMO4 is detected both in the nucleus and cytoplasm. Yeast two-hybrid screen, in vitro binding assays, co-immunoprecipitation, siRNA stable knockdown, reporter assay The Journal of biological chemistry Medium 15677447
2006 LKB1 forms a complex with LMO4, GATA-6, and Ldb1, enhancing GATA-mediated transactivation in a kinase-dependent manner; this complex induces p21 expression via a p53-independent mechanism. Co-immunoprecipitation (complex formation), reporter gene assay (GATA-mediated transactivation, p21 promoter), kinase-dead mutant Biochemical and biophysical research communications Medium 16580634
2008 PPARγ signaling requires LMO4 as an essential cofactor to protect neurons from ischemic injury: LMO4 interacts with PPARγ in a ligand-dependent manner (co-immunoprecipitation, mammalian two-hybrid), promotes RXRα binding to PPARγ, increases PPARγ binding to target DNA, and activates SOD2 promoter. PPARγ agonist failed to reduce cerebral infarction in neuron-specific LMO4-null mice. Co-immunoprecipitation, mammalian two-hybrid, LMO4-null cortical neurons, conditional forebrain knockout (CaMKIIα-Cre/LMO4loxP), focal ischemia model, promoter reporter assay, MnTBAP rescue The Journal of neuroscience High 19020036
2009 LMO4 controls the binary fate choice between excitatory V2a and inhibitory V2b spinal interneurons by regulating LIM complexes: LMO4 induces GABAergic V2b interneurons in collaboration with SCL and inhibits Lhx3 from generating glutamatergic V2a interneurons. LMO4 nucleates assembly of a novel LIM-complex containing SCL, Gata2, and NLI, which activates V2b-specific enhancers. In LMO4;SCL compound mutants, V2a cells increase at the expense of V2b cells. Conditional knockout, compound mutant (LMO4;SCL), enhancer reporter assay, co-immunoprecipitation (complex assembly), in situ hybridization for V2a/V2b markers Neuron High 19323994
2009 LMO4 is a downstream target of ErbB2 and PI3K signaling in breast cancer cells; LMO4 silencing causes G2/M arrest associated with decreased cullin-3 (E3 ubiquitin ligase component), followed by reductions in Cyclin D1 and Cyclin E. LMO4 expression oscillates through the cell cycle with maximum at G2/M, preceding cullin-3 oscillations. Gene expression profiling, siRNA knockdown, cell cycle analysis (FACS), immunoblotting, PI3K inhibitor treatment Oncogene Medium 19648968
2011 LMO4 functions as a co-activator of Neurogenin 2 (NGN2) in the developing cortex: LMO4 and NLI/LDB1 interact with NGN2 simultaneously, forming a multi-protein transcription complex recruited to E-box-containing enhancers of NGN2-target genes. Loss of LMO4 in Lmo4-null embryos impairs neuronal differentiation, and LMO4 expression facilitates NGN2-mediated radial migration of cortical neurons. Co-immunoprecipitation (LMO4-NLI-NGN2 complex), chromatin immunoprecipitation (complex at NGN2-target enhancers), Lmo4-null embryo analysis, in utero electroporation (migration assay) Development High 21652654
2011 LMO4 is a Slug/Snail interacting protein (direct binding demonstrated); LMO4 morpholino knockdown in Xenopus causes loss of neural crest precursor formation, and misexpression of LMO4 modulates Slug-mediated neural crest induction, indicating LMO4 is an essential cofactor in Snail2-mediated neural crest development. Morpholino knockdown (Xenopus), misexpression, co-immunoprecipitation/direct binding assay for LMO4-Slug/Snail interaction Developmental biology Medium 22119055
2011 LMO4 is repressed by Alk transcription in the striatum; LMO4 and ERα are associated with the Alk promoter (chromatin immunoprecipitation), and Alk is an estrogen-responsive gene modulated by LMO4 in the striatum. ERα knockout mice show enhanced cocaine sensitization and increased Alk expression. Chromatin immunoprecipitation (LMO4 and ERα at Alk promoter), ERα knockout, RNA interference in striatum The Journal of neuroscience Medium 21976498
2012 LMO4 positively regulates ryanodine receptor type 2 (RyR2) expression; forebrain-specific LMO4 knockout neurons have severely compromised calcium-induced calcium release (CICR) modulation of afterhyperpolarization (rescued by single-cell LMO4 overexpression), absent caffeine-potentiated RyR-mediated calcium release, defective CICR-facilitated glutamate release, and reduced CA3-CA1 LTP. Conditional forebrain knockout, electrophysiology (patch clamp), two-photon calcium imaging, single-cell overexpression rescue, Morris water maze The Journal of neuroscience High 22442089
2012 LMO4 interacts with p53 (co-immunoprecipitation) and inhibits p53-mediated suppression of breast cancer cell colony formation; p53 induction by adriamycin represses LMO4 expression, indicating LMO4 is a transcriptional target of p53. Co-immunoprecipitation, colony formation assay, RT-PCR/Western blot after ADR treatment Life sciences Low 22906635
2012 LMO4 nitration at tyrosine residues in the cochlea is the primary molecular event in cisplatin-induced ototoxicity: cisplatin induces nitroxidative protein modification and nitration of LMO4 (identified by MALDI-TOF mass spectrometry, confirmed by reciprocal immunoprecipitation and immunoblotting), leading to decreased cochlear LMO4 levels and cochlear apoptosis. MALDI-TOF mass spectrometry, reciprocal co-immunoprecipitation/immunoblotting, immunofluorescence co-localization of nitrotyrosine and LMO4, in vitro nitration assay The Journal of biological chemistry High 22493493
2012 LMO4 is required for normal DEAF1 nuclear export: LMO4 modulates the activity of the DEAF1 nuclear export signal (NES) within a coiled-coil domain, causing nuclear accumulation of constructs containing the LMO4-interaction region of DEAF1. The specific LMO4-binding domain in DEAF1 contains an unstructured region that directly contacts LMO4. Yeast two-hybrid domain mapping, cell-based NES activity assay, co-expression/localization PloS one Medium 22723967
2013 LMO4 is an essential cofactor in Snail2-mediated epithelial-to-mesenchymal transition and cadherin repression in neuroblastoma and neural crest cells; LMO4 knockdown impairs neuroblastoma cell invasion and neural crest delamination, and LMO4 is required for Snail2-mediated cadherin downregulation. siRNA/shRNA knockdown, invasion assay, in vivo chick neural crest delamination assay, cadherin expression analysis The Journal of neuroscience Medium 23407937
2013 HBXIP activates LMO4 transcription by binding to the LMO4 promoter through the transcription factor Sp1 at the −237/−206 region; HBXIP co-immunoprecipitates with Sp1, ChIP shows HBXIP at the LMO4 promoter, and mutation of the Sp1 site abolishes HBXIP-LMO4 promoter interaction. Co-immunoprecipitation, chromatin immunoprecipitation, electrophoretic mobility shift assay, luciferase reporter, Sp1-binding site mutagenesis Carcinogenesis Medium 23291272
2013 The solution NMR structure of LMO4 in complex with CtIP/RBBP8 reveals that CtIP and LDB1 bind to the same face on LMO4 and cannot simultaneously bind LMO4, suggesting competitive binding between these two partners. NMR solution structure determination, competition binding assay Journal of molecular biology High 23353824
2014 Loss of LMO4 in retinal progenitor cells leads to fewer Bhlhb5-positive GABAergic amacrine and OFF-cone bipolar cells (affecting the postnatal wave), with functional deficit shown by reduced b-wave amplitude on electroretinography; cholinergic/dopaminergic amacrine, rod bipolar, and photoreceptor cell numbers are not affected. Conditional knockout (Pax6α-Cre/LMO4flox), cell counting by immunostaining, electroretinography PloS one High 20949055
2014 The NMR solution structure of the LMO4-LIM2/DEAF1 complex shows that DEAF1's intrinsically disordered LMO4-binding domain becomes structured upon binding LMO4, and DEAF1 binds the same face on LMO4 as LDB1 and CtIP; mutagenic screening identified key residues in LMO4-LIM2 and the N-terminal half of DEAF1's binding domain. NMR solution structure, yeast two-hybrid mutagenic screening, domain mapping PloS one High 25310299
2014 LMO4 inactivation in the cochlea causes ectopic formation of an organ of Corti (eOC) in the lateral cochlea with inner/outer hair cells and supporting cells but in mirror-image orientation, demonstrating LMO4 is a negative regulator of sensory organ formation in the cochlea. Conditional knockout (Lmo4 inactivation), histology, hair cell markers (immunostaining) The Journal of neuroscience High 25057208
2014 LMO4 regulates the expression of Ca2+ channel subunits Cacna1h and Cacna1e in PVH neurons; PVH-specific LMO4 ablation reduces voltage-activated Ca2+ currents and basal neuronal excitability, leading to hyperphagia. Restoring neuronal activity by DREADD technology suppresses food intake in these mice. Conditional (PVH-specific) knockout, brain slice electrophysiology (patch clamp for Ca2+ currents), real-time PCR for Ca2+ channel expression, DREADD designer receptor The Journal of neuroscience High 24381275
2014 Loss of LMO4 in hypothalamic glutamatergic neurons impairs insulin signaling in the hypothalamus and peripheral tissues (liver, skeletal muscle), associated with markedly elevated PTP1B activity; administration of a PTP1B inhibitor to the hypothalamus restores insulin signaling and improves peripheral insulin response in LMO4-deficient mice. Conditional knockout (glutamatergic neuron-specific), insulin signaling assays (phospho-IR, phospho-Akt), PTP1B activity assay, intracerebroventricular drug delivery Biochemical and biophysical research communications Medium 24937445
2015 LMO4 is required for cisplatin-induced cochlear apoptosis: hair cell-specific Lmo4 conditional knockout mice show elevated auditory brainstem response thresholds after cisplatin; LMO4 deficiency compromises STAT3 phosphorylation (anti-apoptotic) and increases activated caspase-3 in hair cells post-cisplatin. Conditional knockout (Lmo4lox/lox; Gfi1Cre), auditory brainstem response, immunostaining for phospho-STAT3 and caspase-3 Molecular neurobiology High 33411315
2015 LMO4 in the basolateral amygdala (BLA) is required for cue-reward learning but not fear learning or anxiety; knockdown of LMO4 in BLA (but not nucleus accumbens) impairs conditioned reinforcement; molecular analysis identifies a deficit in dopamine D2 receptor signaling in the BLA of Lmo4-deficient mice. Lmo4-deficient mice, BLA-specific RNA interference, conditioned reinforcement behavioral assay, electrophysiology (D2 receptor signaling) The Journal of neuroscience Medium 26134647
2015 Cisplatin treatment modulates LMO4's downstream cochlear interactome: Esr1 (estrogen receptor 1) is upregulated and Stat3 is downregulated by cisplatin in the cochlea; Trolox co-treatment attenuates these effects, and immunoblots confirm cisplatin-induced decrease in cochlear STAT3 protein is attenuated by Trolox, suggesting LMO4 loss promotes STAT3 repression in cisplatin ototoxicity. Custom gene array, RT-PCR, immunoblotting (STAT3), antioxidant rescue (Trolox co-treatment) in rats PloS one Low 25501662
2018 Lmx1a and Lmo4 mutually negatively regulate each other in the inner ear: Lmo4 negatively regulates Lmx1a to allow formation of the three sensory cristae and blocks ectopic cochlear sensory formation; Lmx1a negatively regulates Lmo4 to mediate epithelial resorption of the canal pouch. Conditional knockout of Lmx1a shows the inner ear source is the major contributor to ear patterning. Conditional knockout of Lmx1a and compound mutant analysis with Lmo4; histology, in situ hybridization for otic markers The Journal of neuroscience High 29769265
2019 Yeats4 recruits the Dot1l-RNA Pol II complex onto the Lmo4 promoter by recognizing H3K27ac modification, initiating Lmo4 transcription in α4β7+ CLPs; Yeats4 conditional KO decreases ILC numbers and Lmo4 expression, and Lmo4 deficiency also impairs ILC lineage differentiation. Conditional knockout, ChIP (Yeats4, Dot1l, RNA Pol II at Lmo4 promoter), H3K27ac recognition assay The Journal of experimental medicine Medium 31434684
2020 LMO4 binds the Oprk1 promoter (chromatin immunoprecipitation) in the basolateral amygdala (BLA), regulating kappa opioid receptor expression; LMO4 also controls extracellular matrix gene expression in the BLA. BLA-specific Lmo4 knockdown decreases alcohol consumption; disruption of ECM or kappa opioid receptor antagonism in the BLA reduces alcohol consumption. Lmo4 heterozygous and shRNA knockdown, RNA-seq, chromatin immunoprecipitation, pharmacological dissection (BLA infusion) Molecular psychiatry Medium 32144357
2021 Lmo4 deficiency enhances susceptibility to cisplatin-induced cochlear apoptosis: hair cell-specific conditional LMO4 deletion does not alter baseline cochlear morphology or hearing but significantly increases ABR thresholds after cisplatin; LMO4 deficiency impairs STAT3 activation, and this LMO4/STAT3 axis is an otoprotective cellular defense mechanism. Conditional knockout (Lmo4lox/lox; Gfi1Cre), ABR, immunostaining (phospho-STAT3, caspase-3) Molecular neurobiology High 33411315
2024 LMO4 binds JAK1 directly and potentiates STAT3 signaling in response to IL-21 in CD8+ T cells, inducing expression of stemness-related target genes (Tcf7, Socs3, Junb, Zfp36). CRISPR/Cas9 deletion of STAT3 nullifies the enhanced memory signature conferred by LMO4. LMO4 overexpression in CD8+ T cells promotes stem-like memory phenotype and antitumor immunity. CRISPR activation screen, synthetic overexpression, co-immunoprecipitation (LMO4-JAK1 interaction), CRISPR/Cas9 Stat3 deletion, syngeneic and xenograft tumor models, transcriptomics Signal transduction and targeted therapy High 39117617
2025 Salmonella effector SsPH2 targets LMO4 for ubiquitination and proteasome-dependent degradation: SsPH2 mediates K48-linked poly-ubiquitination of LMO4 at Lysine 29 and Lysine 67 within the LIM domains (in vivo and in vitro); SsPH2 alters the subcellular localization of LMO4, destabilizes gp130, and inhibits STAT3 activation. Yeast two-hybrid screen, GST pull-down, co-immunoprecipitation, immunofluorescence microscopy, in vitro and in vivo ubiquitination assay, lysine mutational analysis, proteasome inhibitor rescue FASEB journal High 40693870
2025 LMO4 promotes OSCC progression by inducing ubiquitin-proteasome-dependent degradation of the tumor suppressor RAB17, thereby promoting cancer cell proliferation, migration, and resistance to ferroptosis; restoration of RAB17 reduces these malignant behaviors and reduces in vivo tumor growth. siRNA/overexpression, ubiquitin-proteasome pathway assay, xenograft model, immunostaining for RAB17 and LMO4 Cell death & disease Medium 41213908

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 The Grainyhead-like epithelial transactivator Get-1/Grhl3 regulates epidermal terminal differentiation and interacts functionally with LMO4. Developmental biology 152 16949565
2001 The LIM domain protein LMO4 interacts with the cofactor CtIP and the tumor suppressor BRCA1 and inhibits BRCA1 activity. The Journal of biological chemistry 129 11751867
2001 The LIM domain gene LMO4 inhibits differentiation of mammary epithelial cells in vitro and is overexpressed in breast cancer. Proceedings of the National Academy of Sciences of the United States of America 123 11734645
2004 Defective neural tube closure and anteroposterior patterning in mice lacking the LIM protein LMO4 or its interacting partner Deaf-1. Molecular and cellular biology 105 14966286
2014 Combinatorial expression of Lef1, Lhx2, Lhx5, Lhx9, Lmo3, Lmo4, and Prox1 helps to identify comparable subdivisions in the developing hippocampal formation of mouse and chicken. Frontiers in neuroanatomy 90 25071464
1998 Identification and characterization of LMO4, an LMO gene with a novel pattern of expression during embryogenesis. Proceedings of the National Academy of Sciences of the United States of America 89 9736723
1998 Mouse deformed epidermal autoregulatory factor 1 recruits a LIM domain factor, LMO-4, and CLIM coregulators. Proceedings of the National Academy of Sciences of the United States of America 85 9860983
2004 Tandem LIM domains provide synergistic binding in the LMO4:Ldb1 complex. The EMBO journal 84 15343268
2005 Overexpression of LMO4 induces mammary hyperplasia, promotes cell invasion, and is a predictor of poor outcome in breast cancer. Proceedings of the National Academy of Sciences of the United States of America 79 15897450
2004 Null mutation of the Lmo4 gene or a combined null mutation of the Lmo1/Lmo3 genes causes perinatal lethality, and Lmo4 controls neural tube development in mice. Molecular and cellular biology 76 14966285
2019 Circular RNA circBACH2 plays a role in papillary thyroid carcinoma by sponging miR-139-5p and regulating LMO4 expression. Cell death & disease 75 30796202
2006 Calcium activation of the LMO4 transcription complex and its role in the patterning of thalamocortical connections. The Journal of neuroscience : the official journal of the Society for Neuroscience 71 16899735
2009 LMO4 controls the balance between excitatory and inhibitory spinal V2 interneurons. Neuron 69 19323994
1998 Identification of the LMO4 gene encoding an interaction partner of the LIM-binding protein LDB1/NLI1: a candidate for displacement by LMO proteins in T cell acute leukaemia. Oncogene 65 9840944
2003 Structural basis for the recognition of ldb1 by the N-terminal LIM domains of LMO2 and LMO4. The EMBO journal 62 12727888
2013 The oncoprotein HBXIP activates transcriptional coregulatory protein LMO4 via Sp1 to promote proliferation of breast cancer cells. Carcinogenesis 57 23291272
2007 The LIM-only factor LMO4 regulates expression of the BMP7 gene through an HDAC2-dependent mechanism, and controls cell proliferation and apoptosis of mammary epithelial cells. Oncogene 57 17452977
2009 Lmo4 and Clim1 progressively delineate cortical projection neuron subtypes during development. Cerebral cortex (New York, N.Y. : 1991) 54 19366868
2005 The LIM domain-only protein LMO4 is required for neural tube closure. Molecular and cellular neurosciences 52 15691703
2006 LMO4 can interact with Smad proteins and modulate transforming growth factor-beta signaling in epithelial cells. Oncogene 46 16331278
2015 Cisplatin-induced apoptosis in auditory, renal, and neuronal cells is associated with nitration and downregulation of LMO4. Cell death discovery 44 26925255
2003 The LIM-only protein, LMO4, and the LIM domain-binding protein, LDB1, expression in squamous cell carcinomas of the oral cavity. British journal of cancer 44 12771919
2008 Grhl3 and Lmo4 play coordinate roles in epidermal migration. Developmental biology 43 18619436
2012 LIM domain only 4 (LMO4) regulates calcium-induced calcium release and synaptic plasticity in the hippocampus. The Journal of neuroscience : the official journal of the Society for Neuroscience 42 22442089
2008 Rescue of neurons from ischemic injury by peroxisome proliferator-activated receptor-gamma requires a novel essential cofactor LMO4. The Journal of neuroscience : the official journal of the Society for Neuroscience 42 19020036
2005 Mutation analysis of FANCD2, BRIP1/BACH1, LMO4 and SFN in familial breast cancer. Breast cancer research : BCR 41 16280053
2006 The tumor suppressor LKB1 induces p21 expression in collaboration with LMO4, GATA-6, and Ldb1. Biochemical and biophysical research communications 40 16580634
2003 The LIM-only protein LMO4 modulates the transcriptional activity of HEN1. Biochemical and biophysical research communications 39 12878195
2020 Long non‑coding RNA SNHG1 promotes breast cancer progression by regulation of LMO4. Oncology reports 37 32323846
2013 LMO4 is an essential cofactor in the Snail2-mediated epithelial-to-mesenchymal transition of neuroblastoma and neural crest cells. The Journal of neuroscience : the official journal of the Society for Neuroscience 37 23407937
2012 Cisplatin-induced ototoxicity is mediated by nitroxidative modification of cochlear proteins characterized by nitration of Lmo4. The Journal of biological chemistry 37 22493493
2007 Extracellular ATP-dependent upregulation of the transcription cofactor LMO4 promotes neuron survival from hypoxia. Experimental cell research 37 17524392
2009 LMO4 is an essential mediator of ErbB2/HER2/Neu-induced breast cancer cell cycle progression. Oncogene 35 19648968
2009 Requirement for Lmo4 in the vestibular morphogenesis of mouse inner ear. Developmental biology 35 19913004
2005 The LIM domain protein Lmo4 is highly expressed in proliferating mouse epithelial tissues. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 35 15805422
2004 Expression of an engrailed-LMO4 fusion protein in mammary epithelial cells inhibits mammary gland development in mice. Oncogene 35 14676840
2005 Modulation of the interleukin-6 receptor subunit glycoprotein 130 complex and its signaling by LMO4 interaction. The Journal of biological chemistry 32 15677447
2011 LMO4 functions as a co-activator of neurogenin 2 in the developing cortex. Development (Cambridge, England) 30 21652654
2002 Differential expression of a transcription regulatory factor, the LIM domain only 4 protein Lmo4, in muscle sensory neurons. Development (Cambridge, England) 29 12397097
2011 Alk is a transcriptional target of LMO4 and ERα that promotes cocaine sensitization and reward. The Journal of neuroscience : the official journal of the Society for Neuroscience 28 21976498
2011 The LIM adaptor protein LMO4 is an essential regulator of neural crest development. Developmental biology 28 22119055
2016 LIM-Only Protein 4 (LMO4) and LIM Domain Binding Protein 1 (LDB1) Promote Growth and Metastasis of Human Head and Neck Cancer (LMO4 and LDB1 in Head and Neck Cancer). PloS one 26 27780223
2010 Lmo4 in the nucleus accumbens regulates cocaine sensitivity. Genes, brain, and behavior 26 20618444
2007 LMO4 mRNA stability is regulated by extracellular ATP in F11 cells. Biochemical and biophysical research communications 26 17418808
2008 Transcription factor Lmo4 defines the shape of functional areas in developing cortices and regulates sensorimotor control. Developmental biology 25 19111533
2005 Loss of the LIM domain protein Lmo4 in the mammary gland during pregnancy impedes lobuloalveolar development. Oncogene 25 15856027
2011 Ablation of LMO4 in glutamatergic neurons impairs leptin control of fat metabolism. Cellular and molecular life sciences : CMLS 24 21874351
2004 Differential expression of LMO4 protein in Alzheimer's disease. Neuropathology and applied neurobiology 24 14720177
2020 Circ_0058124 Aggravates the Progression of Papillary Thyroid Carcinoma by Activating LMO4 Expression via Targeting miR-370-3p. Cancer management and research 23 33061633
2014 LMO4 is required to maintain hypothalamic insulin signaling. Biochemical and biophysical research communications 23 24937445
2010 Loss of LMO4 in the retina leads to reduction of GABAergic amacrine cells and functional deficits. PloS one 23 20949055
2003 Transcription regulator LMO4 interferes with neuritogenesis in human SH-SY5Y neuroblastoma cells. Brain research. Molecular brain research 23 12877980
2008 Expression of LMO4 and outcome in pancreatic ductal adenocarcinoma. British journal of cancer 22 18231110
1999 Expression of LMO-4 in the central nervous system of the embryonic and adult mouse. Cellular and molecular biology (Noisy-le-Grand, France) 22 10512198
2014 LMO4 functions as a negative regulator of sensory organ formation in the mammalian cochlea. The Journal of neuroscience : the official journal of the Society for Neuroscience 21 25057208
2012 Contribution of DEAF1 structural domains to the interaction with the breast cancer oncogene LMO4. PloS one 21 22723967
2023 Pro-inflammatory cytokine IL-6 regulates LMO4 expression in psoriatic keratinocytes via AKT/STAT3 pathway. Immunity, inflammation and disease 18 38156380
2019 LMO4 promotes the invasion and proliferation of gastric cancer by activating PI3K-Akt-mTOR signaling. American journal of translational research 18 31737204
2018 Reciprocal Negative Regulation Between Lmx1a and Lmo4 Is Required for Inner Ear Formation. The Journal of neuroscience : the official journal of the Society for Neuroscience 18 29769265
2010 Aberrant expression of LMO4 induces centrosome amplification and mitotic spindle abnormalities in breast cancer cells. The Journal of pathology 18 20814902
2014 Downstream targets of Lmo4 are modulated by cisplatin in the inner ear of Wistar rats. PloS one 17 25501662
2013 BRCA1, LMO4, and CtIP mRNA expression in erlotinib-treated non-small-cell lung cancer patients with EGFR mutations. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 17 23407556
2024 LIM-domain-only 4 (LMO4) enhances CD8+ T-cell stemness and tumor rejection by boosting IL-21-STAT3 signaling. Signal transduction and targeted therapy 16 39117617
2020 Differential regulation of alcohol consumption and reward by the transcriptional cofactor LMO4. Molecular psychiatry 16 32144357
2019 Yeats4 drives ILC lineage commitment via activation of Lmo4 transcription. The Journal of experimental medicine 16 31434684
2014 LMO4 is essential for paraventricular hypothalamic neuronal activity and calcium channel expression to prevent hyperphagia. The Journal of neuroscience : the official journal of the Society for Neuroscience 16 24381275
2021 CircLDLR Promotes Papillary Thyroid Carcinoma Tumorigenicity by Regulating miR-637/LMO4 Axis. Disease markers 15 34925640
2020 Lmo4-resistin signaling contributes to adipose tissue-liver crosstalk upon weight cycling. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 15 32030835
2017 LMO4 Is a Disease-Provocative Transcription Coregulator Activated by IL-23 in Psoriatic Keratinocytes. The Journal of investigative dermatology 15 29258893
2012 LMO4 inhibits p53-mediated proliferative inhibition of breast cancer cells through interacting p53. Life sciences 15 22906635
2007 A zebrafish LMO4 ortholog limits the size of the forebrain and eyes through negative regulation of six3b and rx3. Developmental biology 15 17692837
2021 Lmo4 Deficiency Enhances Susceptibility to Cisplatin-Induced Cochlear Apoptosis and Hearing Loss. Molecular neurobiology 14 33411315
2012 Lmo4 in the basolateral complex of the amygdala modulates fear learning. PloS one 14 22509321
2021 FOXM1 Promotes Head and Neck Squamous Cell Carcinoma via Activation of the Linc-ROR/LMO4/AKT/PI3K Axis. Frontiers in oncology 13 34447693
2018 LMO4 mediates trastuzumab resistance in HER2 positive breast cancer cells. American journal of cancer research 13 29736306
2003 Mutational analysis of the LMO4 gene, encoding a BRCA1-interacting protein, in breast carcinomas. International journal of cancer 13 12925972
2020 Cisplatin-induced hair cell loss in zebrafish neuromasts is accompanied by protein nitration and Lmo4 degradation. Toxicology and applied pharmacology 12 33245977
2018 miR-150 might inhibit cell proliferation and promote cell apoptosis by targeting LMO4 in Burkitt lymphoma. Journal of cellular physiology 12 30422313
2009 The highly related LIM factors, LMO1, LMO3 and LMO4, play different roles in the regulation of the pituitary glycoprotein hormone alpha-subunit (alpha GSU) gene. Bioscience reports 12 19228115
2003 Two promoters within the human LMO4 gene contribute to its overexpression in breast cancer cells. Genomics 12 12906853
2010 A novel role for transcription factor Lmo4 in thymus development through genetic interaction with Cited2. Developmental dynamics : an official publication of the American Association of Anatomists 11 20549734
2003 Crystallization of FLINC4, an intramolecular LMO4-ldb1 complex. Acta crystallographica. Section D, Biological crystallography 11 12876360
1999 Characterization of the Lmo4 gene encoding a LIM-only protein: genomic organization and comparative chromosomal mapping. Mammalian genome : official journal of the International Mammalian Genome Society 11 10556429
2022 Human umbilical cord mesenchymal stem cell-derived extracellular vesicles carrying miR-655-3p inhibit the development of esophageal cancer by regulating the expression of HIF-1α via a LMO4/HDAC2-dependent mechanism. Cell biology and toxicology 10 36222945
2014 The structure of an LIM-only protein 4 (LMO4) and Deformed epidermal autoregulatory factor-1 (DEAF1) complex reveals a common mode of binding to LMO4. PloS one 10 25310299
2013 LMO4 modulates proliferation and differentiation of 3T3-L1 preadipocytes. FEBS letters 10 23892074
2022 Nimodipine Treatment Protects Auditory Hair Cells from Cisplatin-Induced Cell Death Accompanied by Upregulation of LMO4. International journal of molecular sciences 9 35628594
2013 Structural basis of the interaction of the breast cancer oncogene LMO4 with the tumour suppressor CtIP/RBBP8. Journal of molecular biology 9 23353824
2023 Lmo4 synergizes with Fezf2 to promote direct in vivo reprogramming of upper layer cortical neurons and cortical glia towards deep-layer neuron identities. PLoS biology 8 37552690
2015 A Selective Role for Lmo4 in Cue-Reward Learning. The Journal of neuroscience : the official journal of the Society for Neuroscience 7 26134647
2015 Lmo4 and Other LIM domain only factors are necessary and sufficient for multiple retinal cell type development. Developmental neurobiology 5 26579872
2013 Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1. Biomolecular NMR assignments 4 23417771
2011 ¹H, ¹⁵N and ¹³C assignments of an intramolecular LMO4-LIM1/CtIP complex. Biomolecular NMR assignments 4 21643835
2002 Dynamic expression and regulation by Fgf8 and Pou2 of the zebrafish LIM-only gene, lmo4. Mechanisms of development 4 14516683
2002 Dynamic expression and regulation by Fgf8 and Pou2 of the zebrafish LIM-only gene, lmo4. Gene expression patterns : GEP 3 12617802
2025 The Type III Secretion Effector SsPH2 of Salmonella enterica Targets LMO4 for Ubiquitination and Degradation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2 40693870
2025 miR-143-3p mediates mercury chloride-induced neurotoxicity by targeting LMO4 and the Akt/GSK3β/mTOR pathway in vitro. Toxicological research 2 41503443
2025 The Protective Effect of Nimodipine in Schwann Cells Is Related to the Upregulation of LMO4 and SERCA3 Accompanied by the Fine-Tuning of Intracellular Calcium Levels. International journal of molecular sciences 1 39859578
2021 Research and Clinical Significance of the Differentially Expressed Genes TP63 and LMO4 in Human Immunodeficiency Virus-Related Penile Squamous Cell Carcinoma. American journal of men's health 1 33906487
2025 LMO4 promotes OSCC progression by inducing RAB17 degradation and ferroptosis resistance. Cell death & disease 0 41213908

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