Affinage

LMAN1

Protein ERGIC-53 · UniProt P49257

Length
510 aa
Mass
57.5 kDa
Annotated
2026-06-10
92 papers in source corpus 44 papers cited in narrative 44 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/8 claims corpus-supported (88%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

LMAN1 (ERGIC-53) is a hexameric type I transmembrane lectin that operates as a glycoprotein cargo receptor in the early secretory pathway, cycling continuously between the ER, ERGIC, and cis-Golgi (PMID:9788882, PMID:16257008). Its short cytoplasmic tail encodes the dual trafficking logic of this cycle: a C-terminal phenylalanine motif that binds the COPII coat component Sec23 to drive ER exit, and a dilysine (KKXX) motif that mediates retrograde retrieval and, upon surface escape, lysine-dependent endocytosis (PMID:8119975, PMID:9395526). Full ER export is reconstituted by three cooperating determinants—the COPII phenylalanine signal, disulfide- and coiled-coil-stabilized hexamerization, and an optimal transmembrane domain length (PMID:13130098). Within the ER lumen, its leguminous-lectin-fold carbohydrate recognition domain binds high-mannose N-glycans with broad, low-affinity specificity in a calcium-dependent manner (PMID:8868475, PMID:11850423, PMID:18025080), and a conserved CRD histidine acts as a pH/Ca2+ sensor so that cargo is captured at ER pH and released in the more acidic ERGIC (PMID:14718532). Through this mechanism LMAN1 selectively promotes ER-to-Golgi transport of a defined subset of secretory glycoproteins, including cathepsin C and cathepsin Z, alpha1-antitrypsin, Mac-2BP, MMP-9, and thrombopoietin (PMID:9679138, PMID:10559958, PMID:18283111, PMID:23550150, PMID:26150355, PMID:39499573). For coagulation factors V and VIII, LMAN1 forms a stoichiometric 1:1 complex with the soluble EF-hand protein MCFD2, which it anchors in the ER and which contributes an additional polypeptide-recognition surface; the MCFD2- and cargo/sugar-binding sites on the CRD are structurally separable (PMID:15886209, PMID:20817851, PMID:20138881, PMID:24498414). Loss-of-function mutations in LMAN1 cause the autosomal recessive bleeding disorder combined deficiency of coagulation factors V and VIII (F5F8D) (PMID:9546392). Beyond its secretory role, LMAN1 is exploited by multiple enveloped viruses for production of infectious particles (PMID:24237698, PMID:32806600) and functions at the cell surface as a receptor for house dust mite allergens that dampens NF-κB signaling (PMID:36870056).

Mechanistic history

Synthesis pass · year-by-year structured walk · 30 steps
  1. 1994 High

    Established that LMAN1's cytoplasmic dilysine motif is the operational signal for pre-Golgi retention, answering how the protein is confined to the early secretory pathway.

    Evidence Site-directed mutagenesis of the KKXX lysines with retention and endocytosis assays in COS cells

    PMID:8119975

    Open questions at the time
    • Did not identify the retrograde coat machinery (COPI) recognizing the motif
    • Surface endocytosis was an overexpression artifact, not the physiological route
  2. 1995 High

    Defined the cytoplasmic domain as necessary and sufficient for cis-Golgi recycling localization and identified an adjacent phenylalanine determinant, resolving the bidirectional targeting code.

    Evidence CD4 reporter domain-swap experiments with endoglycosidase H resistance and immunofluorescence readouts

    PMID:7559786

    Open questions at the time
    • Anterograde coat partner of the phenylalanine motif not yet identified
    • Luminal cargo-binding function not addressed
  3. 1996 High

    Demonstrated that LMAN1 is a functional mannose-selective, calcium-dependent lectin, providing the molecular basis for glycoprotein cargo recognition.

    Evidence Mannose-affinity chromatography of overexpressed protein plus CRD-residue mutagenesis abolishing binding

    PMID:8868475

    Open questions at the time
    • Physiological cargo glycoproteins not yet identified
    • Glycan specificity not quantified
  4. 1997 High

    Showed the C-terminal phenylalanine ER-exit determinant binds COPII Sec23 directly, defining the anterograde transport mechanism.

    Evidence In vitro peptide binding assay with Sec23p plus mutagenesis and trafficking readouts

    PMID:9395526

    Open questions at the time
    • Did not address Sec24 cargo-adaptor contribution
    • Oligomerization requirement for exit not yet established
  5. 1998 High

    Connected LMAN1 to human disease by showing null mutations cause combined factor V and VIII deficiency, establishing its physiological role as an FV/FVIII transport receptor.

    Evidence Positional cloning and sequencing in F5F8D patients with protein-absence confirmation in patient cells

    PMID:9546392

    Open questions at the time
    • Mechanism of FV/FVIII recognition not defined
    • Did not explain why only FV and FVIII are affected
  6. 1998 High

    Demonstrated cargo selectivity by showing that ER mistargeting of LMAN1 impairs cathepsin C secretion specifically, establishing the cargo-receptor concept for a defined glycoprotein subset.

    Evidence Inducible ER-retention mutant in HeLa cells with metabolic labeling and secretion immunoprecipitation

    PMID:9679138

    Open questions at the time
    • Direct cargo binding not shown in this study
    • Full cargo repertoire unknown
  7. 1999 High

    Provided direct mechanistic proof that LMAN1 binds glycoprotein cargo in the ER in a carbohydrate- and calcium-dependent manner and releases it in the ERGIC, separating binding from oligomerization requirements.

    Evidence Co-immunoprecipitation of a cathepsin-Z-related glycoprotein with calcium-depletion, glycan-modification and temperature-block assays

    PMID:10559958

    Open questions at the time
    • Trigger for ERGIC release not yet defined
    • Stoichiometry of cargo capture unknown
  8. 2002 High

    Solved the CRD crystal structure, revealing a leguminous-lectin fold with a ligand-binding cleft and an oligomerization patch, framing the structural basis of lectin and protein-protein function.

    Evidence X-ray crystallography of rat CRD at 1.46 Å (calcium-free)

    PMID:11850423

    Open questions at the time
    • Calcium-bound conformation not captured
    • Glycan-bound complex not resolved
  9. 2003 High

    Captured the calcium-bound CRD, showing two calcium sites and ligand-site conformational changes consistent with high-mannose glycan preference, explaining calcium-dependent cargo capture.

    Evidence X-ray crystallography of the calcium-bound CRD form

    PMID:14643651

    Open questions at the time
    • Did not resolve how calcium loss drives cargo release in cells
    • pH contribution not addressed structurally
  10. 2003 High

    Defined three cooperating ER-export determinants—COPII phenylalanine signal, disulfide/coiled-coil hexamerization, and transmembrane length—reconstituting full transport competence.

    Evidence Systematic mutagenesis with endoglycosidase H resistance and reconstitution of a signal-less construct

    PMID:13130098

    Open questions at the time
    • Did not address luminal cargo loading during assembly
    • Retrograde KKXX coupling not examined
  11. 2004 High

    Identified a pH/Ca2+-sensing CRD histidine as the molecular switch that releases cargo in the slightly acidic ERGIC, resolving the directionality of the capture-release cycle.

    Evidence In vitro mannose-binding at varying pH/Ca2+, histidine mutagenesis and live-cell organelle neutralization with co-IP

    PMID:14718532

    Open questions at the time
    • Did not reconcile pH sensitivity differences seen with mannobiose in later work
    • In vivo pH gradient values inferred indirectly
  12. 2005 High

    Established the hexameric quaternary architecture (three disulfide-linked dimers via coiled coils), defining the functional oligomeric unit of the receptor.

    Evidence Sucrose gradients, cross-linking, non-denaturing gels and cysteine mutagenesis

    PMID:16257008

    Open questions at the time
    • Functional contribution of covalent vs non-covalent hexamers to cargo transport not fully resolved
  13. 2005 High

    Discovered the stoichiometric 1:1 LMAN1-MCFD2 complex and showed LMAN1 retains MCFD2 in the ER, revealing a two-component receptor for FVIII binding.

    Evidence Reciprocal co-IP, cross-linking, stoichiometry analysis and calcium-chelation experiments

    PMID:15886209

    Open questions at the time
    • Relative cargo-binding contributions of LMAN1 vs MCFD2 not yet dissected
    • Binding-site locations on each protein undefined
  14. 2006 High

    Showed MCFD2 is specifically required for FV/FVIII export but dispensable for cathepsin binding, and that LMAN1 anchors MCFD2, defining cargo-class-specific division of labor.

    Evidence siRNA knockdown of LMAN1 and MCFD2 with YFP fragment complementation and localization in vivo

    PMID:17010120

    Open questions at the time
    • Did not establish whether MCFD2 alone can bind/transport cargo
    • Mechanism of cargo discrimination unclear
  15. 2007 High

    Quantified LMAN1 glycan specificity as broad, low-affinity high-mannose binding tunable by single CRD substitutions, refining the lectin recognition model.

    Evidence Frontal affinity chromatography with a pyridylaminated sugar library plus structure-based mutagenesis

    PMID:18025080

    Open questions at the time
    • Did not link specific glycan structures to particular cargo proteins in cells
  16. 2007 High

    Demonstrated cooperative, calcium-dependent LMAN1-MCFD2 binding and that F5F8D MCFD2 missense mutations weaken the interaction, mechanistically linking complex assembly to disease.

    Evidence Flow cytometry with biotinylated soluble LMAN1 and surface plasmon resonance

    PMID:18056485

    Open questions at the time
    • Did not resolve the atomic interface
    • Effect on cargo transport rates not measured
  17. 2008 High

    Identified alpha1-antitrypsin as a conformation- and carbohydrate-dependent LMAN1 cargo via an unbiased complementation screen with KO rescue, broadening the physiological cargo set.

    Evidence YFP fragment complementation cDNA screen, siRNA knockdown and KO cell reconstitution secretion assays

    PMID:18283111

    Open questions at the time
    • MCFD2 involvement in AAT transport not yet tested
    • Specific AAT glycosite not mapped here
  18. 2008 High

    Showed that cargo receptors collectively maintain ERGIC and Golgi architecture by controlling COPI recruitment, placing LMAN1 in a redundant transport-receptor network with Surf4.

    Evidence Single and double siRNA knockdown with live imaging, co-IP and COPI/Golgi marker immunofluorescence

    PMID:18287528

    Open questions at the time
    • Direct vs indirect contribution to COPI recruitment unresolved
    • Whether LMAN1 and Surf4 share cargo not addressed
  19. 2009 High

    Mapped MCFD2's C-terminal EF-hand domains as both necessary and sufficient for LMAN1 binding, with a separable site for FV/FVIII, refining the architecture of the two-component receptor.

    Evidence MCFD2 deletion/missense mutant co-IP plus circular dichroism spectroscopy

    PMID:20007547

    Open questions at the time
    • Atomic-resolution interface not yet solved here
    • Calcium dependence of cargo vs LMAN1 binding only partially separated
  20. 2010 High

    Localized separable MCFD2- and cargo/sugar-binding sites on the LMAN1 CRD and showed oligomerization is required for both ER exit and MCFD2 binding, integrating structure with function.

    Evidence CRD mutagenesis with co-IP, FVIII interaction and oligomerization assays, plus crystal structure of the CRD/MCFD2 complex with CD of disease mutants

    PMID:20138881 PMID:20817851

    Open questions at the time
    • Glycan-bound ternary structure not yet captured
    • Why most F5F8D MCFD2 mutations destabilize rather than block binding still being worked out
  21. 2011 High

    Established an in vivo LMAN1 KO mouse showing ~50% FV/FVIII reductions and ER accumulation of alpha1-antitrypsin, validating physiological cargo relationships in a whole organism.

    Evidence Lman1 knockout mouse plasma factor assays, liver histology/EM and Western blot

    PMID:21795745

    Open questions at the time
    • Partial (not complete) FV/FVIII loss implied redundancy
    • Cathepsin levels unaffected, leaving cargo hierarchy unexplained
  22. 2013 High

    Provided structural and biochemical bases for broad sugar specificity and FV/FVIII recognition, including a ternary glycan complex and identification of critical CRD residues.

    Evidence X-ray crystallography of CRD/MCFD2/mannotriose and CRD/Man-α-1,2-Man complexes with mutagenesis and pH/Ca2+ titration

    PMID:23709226 PMID:24498414

    Open questions at the time
    • Discrepant pH dependence between sugar species not fully reconciled
    • Direct cargo-glycan co-structures absent
  23. 2013 High

    Expanded the role of LMAN1 beyond secretion by showing it is required for production of infectious arenavirus, coronavirus and filovirus particles via lectin-independent association with viral glycoproteins.

    Evidence siRNA knockdown, co-IP, live-cell imaging and viral infectivity/particle assays across virus families

    PMID:24237698

    Open questions at the time
    • Molecular basis of lectin-independent viral GP association undefined
    • Host attachment defect mechanism not resolved
  24. 2015 Medium

    Added MMP-9 to the LMAN1 cargo repertoire, showing N-glycosylation-dependent interaction and KO-dependent secretion defects.

    Evidence Protein fragment complementation, co-IP and LMAN1 KO secretion assays

    PMID:26150355

    Open questions at the time
    • Single lab without reciprocal in vivo validation
    • MCFD2 involvement not tested
  25. 2018 High

    Genetic epistasis in MCFD2 vs LMAN1 KO mice revealed an alternative FVIII secretion pathway and shared roles in alpha1-antitrypsin export, refining the relative contributions of the two components.

    Evidence MCFD2 KO and double-KO mice with plasma factor assays and hepatocyte ER analysis

    PMID:29735583

    Open questions at the time
    • Identity of the alternative FVIII pathway unknown
    • Sex-specific AAT effects unexplained
  26. 2019 Medium

    Identified a glycan-independent role for LMAN1 in trafficking neuronal GABAA and 5HT3A receptors, expanding cargo recognition modes beyond lectin binding.

    Evidence siRNA knockdown, Western blot of LMAN1 KO brain, surface trafficking and co-IP with glycan-independence test

    PMID:30791981

    Open questions at the time
    • Single lab; receptor-class generality unknown
    • Structural basis of glycan-independent recognition undefined
  27. 2022 High

    Demonstrated with CRISPR KO and rescue that the LMAN1-MCFD2 complex transports alpha1-antitrypsin in a glycosylation-site-dependent but MCFD2-independent manner, clarifying cargo-specific requirements.

    Evidence CRISPR KO of LMAN1 and MCFD2, secretion/intracellular assays, rescue and AAT glycosite mutagenesis with co-IP

    PMID:35322856

    Open questions at the time
    • Reconciliation of MCFD2-independence for AAT vs MCFD2-dependence for FVIII not fully mechanistic
  28. 2023 Medium

    Challenged the prevailing model by showing LMAN1 carbohydrate binding is dispensable for FV/FVIII transport and that MCFD2 overexpression alone rescues secretion, recasting LMAN1 as a transmembrane shuttle for MCFD2.

    Evidence Multiple LMAN1/MCFD2 KO cell lines with secretion assays, carbohydrate-binding-mutant and MCFD2-overexpression rescue

    PMID:36490287

    Open questions at the time
    • Single lab; reconciliation with earlier lectin-dependent FV/FVIII data needed
    • Whether MCFD2 alone reaches Golgi without LMAN1 unclear
  29. 2023 Medium

    Revealed a non-secretory surface function for LMAN1 as a house dust mite allergen receptor that dampens NF-κB signaling via FcRγ and SHP1, identifying an immunomodulatory role.

    Evidence Receptor glycocapture screen, direct binding, NF-κB reporter assay, co-IP and SHP1 recruitment assays

    PMID:36870056

    Open questions at the time
    • Single lab; physiological relevance in vivo limited
    • Relationship between surface pool and secretory cycling unclear
  30. 2024 High

    Established LMAN1 as a hepatocyte cargo receptor for thrombopoietin, linking it to platelet homeostasis through an MCFD2-independent mechanism.

    Evidence Tissue-specific Lman1 KO mice with platelet/MK counts, plasma TPO, co-IP and intracellular accumulation assays

    PMID:39499573

    Open questions at the time
    • TPO recognition determinant (glycan vs protein) not fully mapped
    • Why TPO transport is MCFD2-independent unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how LMAN1 selects its diverse cargo through both lectin-dependent and glycan-independent modes, and how the relative roles of carbohydrate binding versus MCFD2 are reconciled across different cargo classes.
  • No unified model explaining glycan-dependent vs glycan-independent cargo recognition
  • Conflicting evidence on whether LMAN1 lectin activity is required for FV/FVIII transport
  • Identity of the alternative MCFD2-independent FVIII secretion route unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0038024 cargo receptor activity 6 GO:0060090 molecular adaptor activity 3
Localization
GO:0005783 endoplasmic reticulum 3 GO:0005794 Golgi apparatus 3 GO:0005886 plasma membrane 2 GO:0031410 cytoplasmic vesicle 2
Pathway
R-HSA-9609507 Protein localization 4 R-HSA-1643685 Disease 3 R-HSA-392499 Metabolism of proteins 3 R-HSA-5653656 Vesicle-mediated transport 3
Partners
ERP44FCRΓMCFD2SEC23SUMF1SURF4UBXD1VIPL
Complex memberships
ERGIC-53 hexamerLMAN1-MCFD2 cargo receptor complex

Evidence

Reading pass · 44 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1993 ERGIC-53 is a type I membrane protein of the ER-Golgi intermediate compartment whose short cytoplasmic tail contains a dilysine (KKXX) motif that functions as an ER retention/retrieval signal, as demonstrated by cDNA cloning and expression in Vero cells. cDNA cloning, sequence analysis, heterologous expression with immunofluorescence localization European journal of cell biology High 8223692
1994 The COOH-terminal dilysine motif of ERGIC-53 mediates both pre-Golgi retention and, when ERGIC-53 reaches the cell surface upon overexpression, lysine-dependent endocytosis; replacing the two critical lysines with serines disrupts both retention and endocytosis. Site-directed mutagenesis, overexpression in COS cells, cell-surface assay, endocytosis assay The Journal of biological chemistry High 8119975
1995 The cytoplasmic domain of ERGIC-53 is required and sufficient for pre-medial-Golgi localization, containing a COOH-terminal dilysine ER-retrieval signal (KKFF) and an adjacent RSQQE targeting determinant; the two C-terminal phenylalanines modulate both signals and are required for full recycling through the ER-ERGIC-cis-Golgi pathway. Domain-swap experiments with CD4 reporter, N-glycosylation/endoglycosidase H resistance assay, immunofluorescence microscopy, site-directed mutagenesis The Journal of cell biology High 7559786
1995 ERGIC-53 is identical to the intracellular mannose-specific lectin MR60 isolated from myelomonocytic cells; sequence homology to leguminous lectins and galectins was established. cDNA cloning, peptide sequence matching, sequence homology analysis The Journal of biological chemistry Medium 7876089
1996 ERGIC-53 is a functional mannose-selective, calcium-dependent lectin. Overexpressed ERGIC-53 binds mannose columns in a calcium-dependent manner; substitution of a conserved asparagine in the putative carbohydrate recognition domain (CRD), or a second CRD-site residue, abolishes mannose binding and co-staining with mannosylated neoglycoprotein. Mannose-affinity chromatography of overexpressed protein, morphological binding assay with mannosylated neoglycoprotein, site-directed mutagenesis Molecular biology of the cell High 8868475
1997 ERGIC-53 carries a C-terminal cytoplasmic phenylalanine-dependent ER-exit determinant that interacts directly with the COPII coat component Sec23p; the two terminal phenylalanines are essential for ER exit and for this interaction. Site-directed mutagenesis, in vitro peptide binding assay with Sec23p, subcellular trafficking assay The Journal of biological chemistry High 9395526
1998 ERGIC-53 cycles continuously through ER, ERGIC, and cis-Golgi; the major retrograde recycling pathway of ERGIC-53 bypasses the Golgi apparatus, returning directly from ERGIC to ER, as shown by temperature-shift experiments and immunogold electron microscopy. Temperature-shift experiments, immunofluorescence microscopy, immunogold electron microscopy, density-gradient centrifugation in HepG2 cells Journal of cell science High 9788882
1998 Mistargeting ERGIC-53 to the ER (by a retention mutant that sequesters endogenous ERGIC-53) specifically impairs secretion of cathepsin C precursor while leaving other lysosomal enzymes and membrane glycoproteins unaffected, establishing that ERGIC-53 recycling is required for efficient transport of a selective subset of glycoproteins. Tetracycline-inducible expression of ER-retention mutant in HeLa cells, metabolic labeling, immunoprecipitation of secreted glycoproteins The Journal of cell biology High 9679138
1998 Mutations in ERGIC-53 (null mutations) cause combined deficiency of coagulation factors V and VIII, identifying ERGIC-53 as an ER-to-Golgi molecular chaperone/transport receptor required for secretion of FV and FVIII. Positional cloning, DNA sequence analysis, immunofluorescence and Western blot of patient lymphocytes Cell High 9546392
1999 ERGIC-53 functions as a cargo transport receptor for glycoproteins: it binds a cathepsin-Z-related glycoprotein in the ER in a carbohydrate- and calcium-dependent manner, and cargo dissociation occurs in the ERGIC. Binding does not require ERGIC-53 oligomerization, but oligomerization is required for ER exit of ERGIC-53 itself. Co-immunoprecipitation, calcium-depletion and glycan-modification experiments, temperature-block assays, mislocalization studies Nature cell biology High 10559958
1999 The sugar-binding ability of ERGIC-53 (MR60) requires a dimeric state: a truncated protein retaining Cys466 (but not Cys475) forms dimers and binds mannosides, whereas a shorter construct lacking both cysteines neither dimerizes nor binds mannose. Expression of recombinant truncated proteins, mannose-column binding, immunoprecipitation/SDS-PAGE oligomerization analysis Glycobiology Medium 10521535
2002 Crystal structure of the carbohydrate recognition domain (CRD) of rat p58/ERGIC-53 determined to 1.46 Å resolution (calcium-free form); the fold resembles leguminous lectins with a beta-sandwich and a negatively charged ligand-binding cleft; a conserved surface patch on the opposite face is implicated in protein-protein interactions and oligomerization. X-ray crystallography at 1.46 Å resolution The Journal of biological chemistry High 11850423
2003 ER export of ERGIC-53 requires three cooperating determinants: (1) a C-terminal phenylalanine motif for COPII interaction, assisted by a cytoplasmic glutamine; (2) disulfide-bond-stabilized hexamerization dependent on polar and aromatic residues in the transmembrane domain; (3) optimal transmembrane domain length of 21 amino acids. Together these reconstitute full transport activity. Site-directed mutagenesis, endoglycosidase H resistance assay, immunofluorescence, reconstitution with signal-less construct Journal of cell science High 13130098
2003 Crystal structure of the CRD of p58/ERGIC-53 in the calcium-bound form reveals two calcium-binding sites 6 Å apart (one novel, one homologous to plant lectins), large conformational changes in the ligand-binding site upon calcium binding, and absence of the short loop present in plant lectins, consistent with preference for Man8GlcNAc2 glycans at ER exit. X-ray crystallography (calcium-bound form) Journal of molecular biology High 14643651
2003 LMAN1 interacts with coagulation factor VIII in vivo via co-immunoprecipitation; the interaction is mediated by both high-mannose N-linked oligosaccharides in the B domain of FVIII and protein-protein contacts. Co-immunoprecipitation from transfected HeLa and COS-1 cells, glycosylation modification experiments Journal of thrombosis and haemostasis : JTH Medium 14629470
2004 pH-induced conversion of ERGIC-53 triggers glycoprotein cargo release: ERGIC-53 binds mannose efficiently at pH 7.4 (ER pH) but not at slightly lower pH (ERGIC pH); a conserved histidine in the CRD center is required for lectin activity and acts as a molecular pH/Ca2+ sensor. Organelle neutralization impairs cargo dissociation in the ERGIC. In vitro mannose-binding assay at varying pH and Ca2+ concentrations, histidine mutagenesis, acidification of live cells, organelle pH neutralization, co-immunoprecipitation The Journal of biological chemistry High 14718532
2005 LMAN1 and MCFD2 form a stoichiometric 1:1 complex in cells; MCFD2 is retained in the ER via its interaction with LMAN1. Both LMAN1 and MCFD2 interact specifically with factor VIII (primarily via the B domain) in a calcium-dependent, glycosylation-independent manner. MCFD2 can interact with FVIII independently of LMAN1-MCFD2 complex formation. Co-immunoprecipitation, cross-linking-immunoprecipitation, stoichiometry analysis, Western blot, calcium chelation experiments The Journal of biological chemistry High 15886209
2005 ERGIC-53 forms exclusively hexameric complexes in cells, existing in two forms: covalent disulfide-linked and non-covalent SDS-sensitive hexamers assembled from three disulfide-linked dimers via coiled-coil interactions. Neither membrane-proximal cysteine is essential for hexamer formation or intracellular ERGIC distribution. Sucrose gradient sedimentation, chemical cross-linking, non-denaturing gel electrophoresis, subcellular fractionation, cysteine mutagenesis Journal of molecular biology High 16257008
2006 MCFD2 is required for the ERGIC-53-dependent ER export of coagulation factors V and VIII but is dispensable for ERGIC-53 binding to cathepsin Z and cathepsin C; in the absence of ERGIC-53, MCFD2 is secreted rather than retained in the ER, establishing ERGIC-53 as the membrane anchor of the complex. siRNA knockdown of ERGIC-53 and MCFD2, YFP fragment complementation assay in vivo, localization studies Traffic (Copenhagen, Denmark) High 17010120
2007 Frontal affinity chromatography shows ERGIC-53 binds high-mannose oligosaccharides with low affinity and broad specificity, not distinguishing between monoglucosylated and deglucosylated high-mannose N-glycans; single amino acid substitutions in the CRD can switch the sugar-binding properties. Frontal affinity chromatography with pyridylaminated sugar library, structure-based mutagenesis The Journal of biological chemistry High 18025080
2007 MCFD2 binding to ERGIC-53 is enhanced when MCFD2 is present; ERGIC-53 sugar binding is enhanced by its interaction with MCFD2 as shown by flow cytometry and surface plasmon resonance; F5F8D patient MCFD2 missense mutations drastically reduce binding affinity to ERGIC-53; the interaction is Ca2+-dependent (weakened below 0.2 mM Ca2+). Flow cytometry with biotinylated soluble ERGIC-53, surface plasmon resonance, endo-H treatment Blood High 18056485
2008 ERGIC-53 is an intracellular transport receptor for alpha1-antitrypsin (α1-AT): ERGIC-53 binds α1-AT in a carbohydrate- and conformation-dependent manner; ERGIC-53 knockdown and knockout cells show a specific secretion defect of α1-AT that is corrected by ERGIC-53 re-expression. YFP-based protein fragment complementation screening of cDNA library, siRNA knockdown, ERGIC-53 KO cell reconstitution, secretion assay The Journal of cell biology High 18283111
2008 SUMF1 interacts with ERGIC-53 in the early secretory pathway; ERGIC-53 favors SUMF1 export from the ER; silencing ERGIC-53 causes proteasomal degradation of SUMF1. Co-immunoprecipitation, siRNA silencing with SUMF1 trafficking and stability assays Human molecular genetics Medium 18508857
2008 Surf4 interacts with ERGIC-53; co-silencing of Surf4 and ERGIC-53 (but not either alone) reduces the number of ERGIC clusters and fragments the Golgi, partially redistributing COPI but not Golgi matrix proteins, establishing that cargo receptors collectively maintain ERGIC and Golgi architecture by controlling COPI recruitment. siRNA knockdown (single and double), co-immunoprecipitation, live imaging of ERGIC stability, immunofluorescence for COPI and Golgi markers, BFA resistance assay Molecular biology of the cell High 18287528
2009 The C-terminal EF-hand domains of MCFD2 are both necessary and sufficient for interaction with LMAN1; these same EF-hand domains also mediate interaction with FV and FVIII but via a site separable from the LMAN1-binding site; Ca2+-induced folding is important for LMAN1 interaction but not for FV/FVIII binding. MCFD2 deletion and missense mutant analysis, co-immunoprecipitation, circular dichroism spectroscopy Blood High 20007547
2010 The LMAN1 CRD contains distinct, separable binding sites for MCFD2 (N-terminal beta sheet) and for FV/FVIII cargo (Ca2+- and sugar-binding sites); monomeric LMAN1 mutants are defective in ER exit and cannot interact with MCFD2, indicating oligomerization is necessary for cargo receptor function. Mutagenesis of LMAN1 CRD, co-immunoprecipitation, FVIII interaction assays, oligomerization analysis Blood High 20817851
2010 Crystal structure of the LMAN1-CRD/MCFD2 complex reveals the protein-protein interaction interface; circular dichroism shows that most F5F8D missense mutations in MCFD2 cause global destabilization, while stable mutations map to the LMAN1-binding surface. X-ray crystallography of LMAN1-CRD/MCFD2 complex, circular dichroism of MCFD2 mutants FEBS letters High 20138881
2011 LMAN1-deficient mice exhibit ~50% reductions in plasma FV and FVIII and platelet FV; ER in hepatocytes is slightly distended with accumulation of α1-antitrypsin and GRP78; no significant effect on cathepsin C or Z levels in liver or α1-antitrypsin in plasma under normal conditions. Lman1 knockout mouse analysis, plasma coagulation factor assays, liver histology and EM, protein level analysis by Western blot Blood High 21795745
2012 UBXD1 interacts with ERGIC-53 via the N-terminal 10 residues of UBXD1 and the C-terminal cytoplasmic tail of ERGIC-53; this interaction requires p97 ATPase activity but not ubiquitin modification; UBXD1 modulates subcellular trafficking of ERGIC-53 including promoting its movement to the cell membrane. LC-MS/MS interactome, co-immunoprecipitation, SILAC proteomic profiling, localization studies, p97/E1 inhibitor experiments Molecular & cellular proteomics : MCP Medium 22337587
2012 Under ER stress, ERGIC-53 redistributes from broad ER/Golgi distribution to compact Golgi localization; this redistribution is abrogated by co-expression of VIPL; ERGIC-53 co-precipitates with VIPL but not VIP36, indicating VIPL interaction regulates ERGIC-53 localization. Monoclonal antibody generation, immunostaining, flow cytometry, co-immunoprecipitation, UPR induction with tunicamycin Glycobiology Medium 22821029
2013 Crystal structure of ERGIC-53-CRD complexed with MCFD2 and α1,2-mannotriose reveals that ERGIC-53 can bind the D1 trimannosyl arm in two alternative modes; a single Asp-to-Gly substitution creates a shallower sugar-binding pocket compared to VIP36, enabling ERGIC-53 to accommodate terminal glucose residues. X-ray crystallography of ternary complex (ERGIC-53-CRD/MCFD2/mannotriose) PloS one High 24498414
2013 Crystal structures of LMAN1-CRD bound to Man-α-1,2-Man define the central mannose-binding site; mutagenesis identifies His178 and Gly251/252 as critical for FV/FVIII binding; mannobiose binding is relatively pH-independent but sensitive to lowered Ca2+ concentrations, suggesting Ca2+ regulates cargo release. X-ray crystallography, site-directed mutagenesis, in vitro binding assays, pH and Ca2+ titration The Journal of biological chemistry High 23709226
2013 ERGIC-53 is required for production of infectious arenavirus, coronavirus, and filovirus particles; ERGIC-53 associates with viral glycoproteins through a lectin-independent mechanism, traffics to budding sites, and is incorporated into virions; in its absence, GP-containing virus particles form but are non-infectious due to impaired host-cell attachment. siRNA knockdown, co-immunoprecipitation, live-cell imaging, viral infectivity and particle formation assays Cell host & microbe High 24237698
2013 Mac-2BP (Mac-2 binding protein) is a novel ERGIC-53 cargo glycoprotein; interaction requires high-mannose-type N-glycan binding by ERGIC-53; ERGIC-53 ER-mistargeting mutant blocks Mac-2BP transport; MCFD2 is also involved in Mac-2BP secretion. GFP fragment complementation cDNA library screen, N-glycan-binding-deficient mutant (N156A), ER-mistargeting mutant (KKAA), glycosylation inhibitors, co-immunoprecipitation Glycobiology Medium 23550150
2015 LMAN1 interacts with N-glycosylated MMP-9 in the ER and is required for efficient MMP-9 secretion; N-glycosylation-deficient MMP-9 is secretion-compromised; LMAN1 knockout cells show reduced MMP-9 secretion. Protein fragment complementation assay, co-immunoprecipitation, LMAN1 KO cell secretion assay Biochemical and biophysical research communications Medium 26150355
2018 MCFD2-deficient mice have lower plasma FV and FVIII than LMAN1-deficient mice; doubly deficient mice match LMAN1-deficient levels, suggesting an alternative FVIII secretion pathway exists. Both LMAN1 and MCFD2 deficiency cause decreased plasma α1-antitrypsin in male mice and comparable ER accumulation of AAT in hepatocytes. Mouse gene targeting (MCFD2 KO), plasma coagulation factor assays, comparison of singly and doubly deficient mice, hepatocyte ER analysis Blood advances High 29735583
2019 LMAN1 promotes surface trafficking of GABAAR β3 subunits in mouse hypothalamic neurons; LMAN1 KO mice show decreased total protein levels of 5HT3A receptors and GABAAR γ2 subunits; LMAN1 interacts with GABAARs in a glycan-independent manner; LMAN1 KO upregulates ERp44 without changing calnexin. siRNA knockdown, Western blot of brain homogenates from LMAN1 KO mice, surface trafficking assay, co-immunoprecipitation with glycan-independence test Biochemical and biophysical research communications Medium 30791981
2020 HBV exploits ERGIC-53 for viral particle propagation; ERGIC-53 interacts with the N146-glycan of the HBV envelope in a productive, lectin-dependent manner; ERGIC-53 silencing blocks infectious viral particle exit but not subviral particle exit; ERGIC-53 acts after nucleocapsid envelopment in conjunction with ESCRT components. siRNA silencing, molecular interaction studies, cell imaging in HBV-expressing liver cells, particle infectivity assays Cells Medium 32806600
2020 Multiple crystal forms of the ERGIC-53-CRD/MCFD2 complex at up to 1.60 Å resolution reveal that MCFD2 (but not ERGIC-53-CRD) exhibits significant conformational plasticity potentially enabling accommodation of diverse polypeptide cargo ligands. X-ray crystallography (multiple crystal forms, 1.60 Å best resolution) Acta crystallographica. Section F, Structural biology communications High 32356523
2021 ERp44 binds ERGIC-53 in the ER to negotiate preferential loading into COPII vesicles; silencing ERGIC-53 causes secretion of Prdx4 (an ERp44-retained client), establishing that ERGIC-53 couples transport of cargo and quality-control inspector proteins. Co-immunoprecipitation, ERGIC-53 siRNA silencing with Prdx4 secretion readout, 4-phenylbutyrate COPII competition iScience Medium 33763635
2022 The LMAN1-MCFD2 complex is a cargo receptor for AAT ER-to-Golgi transport: LMAN1 and MCFD2 KO HepG2 and HEK293T cells show reduced AAT secretion and elevated intracellular AAT due to delayed ER-to-Golgi transport; rescue requires wild-type but not mutant proteins; elimination of the second glycosylation site of AAT abolishes LMAN1-dependent secretion; AAT interaction with LMAN1 is independent of MCFD2. CRISPR KO of LMAN1 and MCFD2, secretion assays, intracellular AAT quantification, rescue with wild-type and mutant proteins, glycosylation site mutagenesis, co-immunoprecipitation The Biochemical journal High 35322856
2023 LMAN1 carbohydrate binding is not essential for FV/FVIII transport; overexpression of MCFD2 alone (wild-type or mutant) rescues FV/FVIII secretion in LMAN1-deficient cells, suggesting MCFD2 performs cargo binding/transport and LMAN1 primarily functions as a transmembrane shuttling carrier for MCFD2. Multiple LMAN1/MCFD2 KO cell lines, FV/FVIII secretion assays, carbohydrate-binding mutant rescue, MCFD2 overexpression rescue Blood advances Medium 36490287
2023 LMAN1 directly binds house dust mite (HDM) allergens on the surface of dendritic cells and airway epithelial cells; LMAN1 overexpression downregulates NF-κB signaling in response to HDM or inflammatory cytokines; HDM promotes LMAN1 binding to FcRγ and recruitment of SHP1. Receptor glycocapture screen, direct binding verification, overexpression NF-κB reporter assay, co-immunoprecipitation (LMAN1-FcRγ), SHP1 recruitment assay, in vivo localization Cell reports Medium 36870056
2024 LMAN1 is a cargo receptor for thrombopoietin (TPO): hepatocyte-specific (but not hematopoietic) LMAN1 deletion causes thrombocytopenia with reduced plasma TPO despite normal Tpo mRNA; TPO co-immunoprecipitates with LMAN1; TPO accumulates intracellularly in LMAN1-deleted cells; TPO secretion is MCFD2-independent. Conditional hepatocyte-specific and hematopoietic-specific Lman1 KO mice, platelet and MK quantification, plasma TPO measurement, co-immunoprecipitation, intracellular TPO accumulation assay JCI insight High 39499573

Source papers

Stage 0 corpus · 92 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1998 Mutations in the ER-Golgi intermediate compartment protein ERGIC-53 cause combined deficiency of coagulation factors V and VIII. Cell 353 9546392
1999 The lectin ERGIC-53 is a cargo transport receptor for glycoproteins. Nature cell biology 271 10559958
2000 ERGIC-53 and traffic in the secretory pathway. Journal of cell science 268 10652252
1997 The recycling of ERGIC-53 in the early secretory pathway. ERGIC-53 carries a cytosolic endoplasmic reticulum-exit determinant interacting with COPII. The Journal of biological chemistry 227 9395526
1998 The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment. Journal of cell science 167 9788882
1996 ERGIC-53 is a functional mannose-selective and calcium-dependent human homologue of leguminous lectins. Molecular biology of the cell 147 8868475
1993 ERGIC-53, a membrane protein of the ER-Golgi intermediate compartment, carries an ER retention motif. European journal of cell biology 146 8223692
1998 Mistargeting of the lectin ERGIC-53 to the endoplasmic reticulum of HeLa cells impairs the secretion of a lysosomal enzyme. The Journal of cell biology 137 9679138
2007 Molecular basis of sugar recognition by the human L-type lectins ERGIC-53, VIPL, and VIP36. The Journal of biological chemistry 121 18025080
1989 Identification of a neutralizing epitope on glycoprotein gp58 of human cytomegalovirus. Journal of virology 119 2467992
1993 Fine specificity of the human immune response to the major neutralization epitopes expressed on cytomegalovirus gp58/116 (gB), as determined with human monoclonal antibodies. Journal of virology 117 7678304
2005 LMAN1 and MCFD2 form a cargo receptor complex and interact with coagulation factor VIII in the early secretory pathway. The Journal of biological chemistry 114 15886209
2008 Identification of ERGIC-53 as an intracellular transport receptor of alpha1-antitrypsin. The Journal of cell biology 109 18283111
1995 Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway. The Journal of cell biology 107 7559786
2008 The cargo receptors Surf4, endoplasmic reticulum-Golgi intermediate compartment (ERGIC)-53, and p25 are required to maintain the architecture of ERGIC and Golgi. Molecular biology of the cell 98 18287528
1995 ERGIC-53, a membrane protein of the endoplasmic reticulum-Golgi intermediate compartment, is identical to MR60, an intracellular mannose-specific lectin of myelomonocytic cells. The Journal of biological chemistry 98 7876089
2004 pH-induced conversion of the transport lectin ERGIC-53 triggers glycoprotein release. The Journal of biological chemistry 93 14718532
2005 Combined deficiency of factor V and factor VIII is due to mutations in either LMAN1 or MCFD2. Blood 88 16304051
2003 ER export of ERGIC-53 is controlled by cooperation of targeting determinants in all three of its domains. Journal of cell science 87 13130098
1990 The gp116 of the gp58/116 complex of human cytomegalovirus represents the amino-terminal part of the precursor molecule and contains a neutralizing epitope. The Journal of general virology 80 1700066
1999 Molecular analysis of the ERGIC-53 gene in 35 families with combined factor V-factor VIII deficiency. Blood 74 10090934
1992 A continuous sequence of more than 70 amino acids is essential for antibody binding to the dominant antigenic site of glycoprotein gp58 of human cytomegalovirus. Journal of virology 73 1323695
2002 Crystal structure of the carbohydrate recognition domain of p58/ERGIC-53, a protein involved in glycoprotein export from the endoplasmic reticulum. The Journal of biological chemistry 70 11850423
1999 ERGIC-53 gene structure and mutation analysis in 19 combined factors V and VIII deficiency families. Blood 68 10090935
2006 Cargo selectivity of the ERGIC-53/MCFD2 transport receptor complex. Traffic (Copenhagen, Denmark) 66 17010120
1991 Use of the polymerase chain reaction to analyse sequence variation within a major neutralizing epitope of glycoprotein B (gp58) in clinical isolates of human cytomegalovirus. The Journal of general virology 66 1714945
2013 The intracellular cargo receptor ERGIC-53 is required for the production of infectious arenavirus, coronavirus, and filovirus particles. Cell host & microbe 61 24237698
2008 Multistep, sequential control of the trafficking and function of the multiple sulfatase deficiency gene product, SUMF1 by PDI, ERGIC-53 and ERp44. Human molecular genetics 59 18508857
2006 ER storage diseases: a role for ERGIC-53 in controlling the formation and shape of Russell bodies. Journal of cell science 52 16735443
1995 Segregation of ERGIC53 and the mammalian KDEL receptor upon exit from the 15 degrees C compartment. European journal of cell biology 52 8690019
2010 Molecular basis of LMAN1 in coordinating LMAN1-MCFD2 cargo receptor formation and ER-to-Golgi transport of FV/FVIII. Blood 50 20817851
2003 LMAN1 is a molecular chaperone for the secretion of coagulation factor VIII. Journal of thrombosis and haemostasis : JTH 47 14629470
2007 The sugar-binding ability of ERGIC-53 is enhanced by its interaction with MCFD2. Blood 46 18056485
2011 Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of α1-antitrypsin. Blood 44 21795745
2003 The crystal structure of the carbohydrate-recognition domain of the glycoprotein sorting receptor p58/ERGIC-53 reveals an unpredicted metal-binding site and conformational changes associated with calcium ion binding. Journal of molecular biology 44 14643651
2013 Structural characterization of carbohydrate binding by LMAN1 protein provides new insight into the endoplasmic reticulum export of factors V (FV) and VIII (FVIII). The Journal of biological chemistry 42 23709226
2019 LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors. Biochemical and biophysical research communications 33 30791981
1994 A dual role for COOH-terminal lysine residues in pre-Golgi retention and endocytosis of ERGIC-53. The Journal of biological chemistry 32 8119975
2012 Protein interaction profiling of the p97 adaptor UBXD1 points to a role for the complex in modulating ERGIC-53 trafficking. Molecular & cellular proteomics : MCP 30 22337587
2013 Regulation of Mac-2BP secretion is mediated by its N-glycan binding to ERGIC-53. Glycobiology 29 23550150
2012 Subcellular localization of ERGIC-53 under endoplasmic reticulum stress condition. Glycobiology 29 22821029
2009 A review of ERGIC-53: its structure, functions, regulation and relations with diseases. Histology and histopathology 29 19609866
2005 Oligomerization and interacellular localization of the glycoprotein receptor ERGIC-53 is independent of disulfide bonds. Journal of molecular biology 29 16257008
2015 LMAN1 (ERGIC-53) is a potential carrier protein for matrix metalloproteinase-9 glycoprotein secretion. Biochemical and biophysical research communications 26 26150355
2007 Regulation of ERGIC-53 gene transcription in response to endoplasmic reticulum stress. The Journal of biological chemistry 26 17535801
2001 ERGL, a novel gene related to ERGIC-53 that is highly expressed in normal and neoplastic prostate and several other tissues. Gene 26 11255007
2014 Structural basis for disparate sugar-binding specificities in the homologous cargo receptors ERGIC-53 and VIP36. PloS one 25 24498414
2003 The cargo receptor ERGIC-53 is a target of the unfolded protein response. Biochemical and biophysical research communications 25 12727195
2021 A virtuous cycle operated by ERp44 and ERGIC-53 guarantees proteostasis in the early secretory compartment. iScience 24 33763635
2020 Hepatitis B Virus Exploits ERGIC-53 in Conjunction with COPII to Exit Cells. Cells 24 32806600
2009 EF-hand domains of MCFD2 mediate interactions with both LMAN1 and coagulation factor V or VIII. Blood 23 20007547
2004 Heat shock induces preferential translation of ERGIC-53 and affects its recycling pathway. The Journal of biological chemistry 23 15292203
2010 Crystal structure of the LMAN1-CRD/MCFD2 transport receptor complex provides insight into combined deficiency of factor V and factor VIII. FEBS letters 22 20138881
2009 High frequency of LMAN1 abnormalities in colorectal tumors with microsatellite instability. Cancer research 20 19118014
2007 Deletion of 3 residues from the C-terminus of MCFD2 affects binding to ERGIC-53 and causes combined factor V and factor VIII deficiency. Blood 20 17971482
2005 Mutations in the MCFD2 gene and a novel mutation in the LMAN1 gene in Indian families with combined deficiency of factor V and VIII. American journal of hematology 20 16044454
2018 Analysis of MCFD2- and LMAN1-deficient mice demonstrates distinct functions in vivo. Blood advances 19 29735583
2006 N-glycosylation of human nicastrin is required for interaction with the lectins from the secretory pathway calnexin and ERGIC-53. Biochimica et biophysica acta 18 16938437
2004 A mutation in LMAN1 (ERGIC-53) causing combined factor V and factor VIII deficiency is prevalent in Jews originating from the island of Djerba in Tunisia. Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis 18 15166951
2022 LMAN1-MCFD2 complex is a cargo receptor for the ER-Golgi transport of α1-antitrypsin. The Biochemical journal 16 35322856
1998 Dynamic recycling of ERGIC53 between the endoplasmic reticulum and the Golgi complex is disrupted by nordihydroguaiaretic acid. Biochemical and biophysical research communications 16 9918822
2001 Molecular characterization of the ERGIC-53 gene in two Japanese patients with combined factor V-factor VIII deficiency. Annals of hematology 15 11446732
2009 A novel missense mutation causing abnormal LMAN1 in a Japanese patient with combined deficiency of factor V and factor VIII. American journal of hematology 13 19787799
2023 Separate roles of LMAN1 and MCFD2 in ER-to-Golgi trafficking of FV and FVIII. Blood advances 10 36490287
2023 LMAN1 is a receptor for house dust mite allergens. Cell reports 9 36870056
2013 Successful percutaneous coronary intervention in a patient with combined deficiency of FV and FVIII due to novel compound heterozygous mutations in LMAN1. Haemophilia : the official journal of the World Federation of Hemophilia 9 23557496
2022 Effect of co-overexpression of the cargo receptor ERGIC-53/MCFD2 on antibody production and intracellular IgG secretion in recombinant Chinese hamster ovary cells. Journal of bioscience and bioengineering 8 35963666
2020 Crystallographic snapshots of the EF-hand protein MCFD2 complexed with the intracellular lectin ERGIC-53 involved in glycoprotein transport. Acta crystallographica. Section F, Structural biology communications 8 32356523
2002 Expression, purification, refolding and crystallization of the carbohydrate-recognition domain of p58/ERGIC-53, an animal C-type lectin involved in export of glycoproteins from the endoplasmic reticulum. Acta crystallographica. Section D, Biological crystallography 8 11856848
2001 ERGIC-53 KKAA signal mediates endoplasmic reticulum retrieval in yeast. European journal of cell biology 8 11302519
2023 Identification of LMAN1- and SURF4-Dependent Secretory Cargoes. Journal of proteome research 7 37844105
1999 The sugar binding activity of MR60, a mannose-specific shuttling lectin, requires a dimeric state. Glycobiology 7 10521535
2022 Combined Factor V and VIII Deficiency with LMAN1 Mutation: A Report of 3 Saudi Siblings. The American journal of case reports 6 36116005
2020 Altered phenotype in LMAN1-deficient mice with low levels of residual LMAN1 expression. Blood advances 6 33196840
2007 A new case of combined factor V and factor VIII deficiency further suggests that the LMAN1 M1T mutation is a frequent cause in Italian patients. Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis 6 17287640
1999 Sequence and expression of the monkey homologue of the ER-golgi intermediate compartment lectin, ERGIC-53. Biochimica et biophysica acta 6 10542336
2008 Transient dimerization and interaction with ERGIC-53 occur in the fibroblast growth factor receptor 3 early secretory pathway. The international journal of biochemistry & cell biology 5 18577465
2014 In vivo function of the ER-Golgi transport protein LMAN1 in photoreceptor homeostasis. Advances in experimental medicine and biology 3 24664723
2010 [Combined deficiency of factors V and VIII caused by a novel compound heterozygous mutation of gene Lman1]. Zhongguo shi yan xue ye xue za zhi 3 20137144
1996 Expression of a human cytomegalovirus gp58 antigenic domain fused to the hepatitis B virus nucleocapsid protein. FEMS immunology and medical microbiology 3 9116635
2019 Crystal structure of the legume lectin-like domain of an ERGIC-53-like protein from Entamoeba histolytica. Acta crystallographica. Section F, Structural biology communications 2 30839295
2013 The lectin ERGIC-53 goes viral. Cell host & microbe 2 24237693
2010 Two new mutations at ERGIC-53 gene in a Turkish family. Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis 2 20460353
2024 RNAi targeting LMAN1-MCFD2 complex promotes anticoagulation in mice. Journal of thrombosis and thrombolysis 1 39222205
2024 LMAN1 serves as a cargo receptor for thrombopoietin. JCI insight 1 39499573
2023 Identification of LMAN1 and SURF4 dependent secretory cargoes. bioRxiv : the preprint server for biology 1 37066360
2022 Molecular cloning, sequence characterization, and expression analysis of C-type lectin (CTL) and ER-Golgi intermediate compartment 53-kDa protein (ERGIC-53) homologs from the freshwater prawn, Macrobrachium rosenbergii. Aquaculture international : journal of the European Aquaculture Society 1 35153391
1999 Intradermal DNA immunization: antisera specific for the membrane lectin MR60/ERGIC-53. Bioscience reports 1 10841272
2026 A dual role for ER-Golgi cargo receptor LMAN1 in supporting CSFV replication and restraining RLR signaling. Journal of virology 0 41773859
2026 Zinc-mediated structural and functional regulation of ERp44 and ERGIC-53 in protein quality control. Journal of biochemistry 0 42083771
2025 Sex-dependent influence of LMAN1 on allergen-induced airway hyperresponsiveness. Journal of immunology (Baltimore, Md. : 1950) 0 40517435
2024 A Novel Variant of the LMAN1 Gene in Combined Factor V and Factor VIII Deficiency in a Saudi Female Child: A Case Report. Cureus 0 38659545

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