- Length
- 539 aa
- Mass
- 60.7 kDa
- Annotated
- 2026-04-28
50 papers in source corpus
10 papers cited in narrative
10 extracted findings
Mechanistic narrative
Synthesis pass · prose summary of the discoveries below
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Mechanism profile
Synthesis pass · controlled-vocabulary classification · explore literature graph →
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Evidence
Reading pass · 10 per-paper findings extracted from the source corpus
| Year | Finding | Method | Journal | Conf | PMIDs |
|---|---|---|---|---|---|
| 2014 | Crystal structure of the human KPNA5 C-terminus in complex with Ebola virus VP24 (eVP24) revealed that eVP24 recognizes a unique nonclassical NLS binding site on KPNA5 that is necessary for efficient PY-STAT1 nuclear transport. eVP24 binds KPNA5 with very high affinity at this site to competitively inhibit PY-STAT1 nuclear import without affecting classical NLS cargo transport. | X-ray crystallography of KPNA5 C-terminus/eVP24 complex; binding affinity measurements; cell-based nuclear transport assays | Cell host & microbe | High | 25121748 |
| 2015 | Lloviu virus VP24 (LLOV VP24) blocks tyrosine-phosphorylated STAT1 (PY-STAT1) binding to KPNA5, preventing STAT1 nuclear accumulation and IFN-induced gene expression, demonstrating that KPNA5's role in PY-STAT1 nuclear import is targeted by multiple filovirus VP24 proteins. | Co-immunoprecipitation; nuclear accumulation assays; IFN-induced gene expression assays in human and bat cells | Virology | Medium | 26255028 |
| 2015 | PHB2 interacts with KPNA1, KPNA5, and KPNA6, and these interactions mediate estrogen-dependent translocation of PHB2 into the nuclei of breast cancer cells. BIG3 blocks the KPNA-binding region(s) of PHB2, thereby inhibiting KPNA-mediated PHB2 nuclear translocation. | Co-immunoprecipitation; siRNA knockdown of individual KPNAs; nuclear translocation assays in breast cancer cells | PloS one | Medium | 26052702 |
| 2017 | VP24 proteins from different Ebolavirus species (EBOV, BDBV, RESTV) all bind KPNA1, KPNA5, and KPNA6, but with differing affinities; BDBV VP24 shows ~10-fold lower KPNA5 binding affinity than EBOV or RESTV VP24. Reduced VP24-KPNA binding affinity decreases IFN inhibition and shortens VP24 half-life, revealing that KPNA interaction stabilizes VP24. | Binding affinity measurements with purified proteins; cell-based VP24 half-life assays; IFN-induced gene expression assays; alanine-scanning mutagenesis | Journal of virology | High | 27974555 |
| 2017 | Molecular dynamics simulations of the VP24-KPNA5 complex identified key hydrogen bonds (D480-T138, E483-R137, D205-R396) critical for the interaction interface. VP24 residues R137 and T138, and KPNA5 residues R396, R398, R480, Y477, and F484, were identified as hotspot residues essential for complex stability. | All-atom molecular dynamics simulation; MM-GB/SA energy calculation; computational alanine scanning | Molecular bioSystems | Low | 28418440 |
| 2017 | Macrocyclic peptide inhibitors of the VP24-KPNA5 protein-protein interaction were identified with dissociation constants in the single-digit nanomolar range for eVP24, and these peptides successfully disrupted the eVP24-KPNA interaction, providing the first chemical probes capable of modulating this PPI. | RaPID (Random non-standard Peptides Integrated Discovery) system; binding affinity measurements; PPI disruption assays with purified proteins | Organic & biomolecular chemistry | Medium | 28574091 |
| 2025 | miR-153 directly targets two binding sites in the 3'UTR of KPNA5 mRNA to suppress KPNA5 expression. KPNA5 facilitates nuclear translocation of NRF2 by binding to three nuclear localization sequences (NLS) of NRF2, and miR-153-mediated suppression of KPNA5 impairs NRF2-mediated antioxidant responses, promoting oxidative stress and cognitive deficits in chronic cerebral hypoperfusion models. | Luciferase reporter assays for miR-153 binding sites; Co-IP for KPNA5-NRF2 interaction; knockdown and overexpression in vivo (rat basal forebrain) and in vitro; nuclear fractionation assays | The Journal of neuroscience | Medium | 40816766 |
| 2025 | KPNA5 recognizes nuclear localization signals (NLS) in the protein tyrosine phosphatase PTPN4 and mediates its nuclear transport. Nuclear PTPN4 inhibits STAT3 phosphorylation and its downstream signaling pathway. KPNA5 overexpression suppressed ovarian cancer cell proliferation, tumor growth, and invasion in vitro and in vivo. | Co-IP for KPNA5-PTPN4 interaction; Western blot for STAT3 phosphorylation; xenograft tumor model; CCK-8, colony formation, Transwell assays | Cancer medicine | Medium | 40145330 |
| 2025 | miR-153-3p derived from bone marrow mesenchymal stem cell exosomes targets KPNA5, and this targeting promotes M2 macrophage polarization to facilitate wound healing. | Target prediction and validation; co-culture assays; exosome transfer experiments; macrophage polarization assays | Applied biochemistry and biotechnology | Low | 40053206 |
| 2025 | Nuclear trafficking of eVP24 by KPNA5 requires simultaneous binding of cellular NLS-containing cargo; eVP24 binding to KPNA5 does not impair cargo NLS binding at the major NLS binding site, indicating that eVP24 and classical cargo can simultaneously associate with KPNA5 for co-transport into the nucleus. | Hydrogen-deuterium exchange mass spectrometry (HDX-MS); native MS; biophysical binding assays with purified proteins; nuclear localization assays | Viruses | Medium | 40872766 |
Source papers
Stage 0 corpus · 50 papers · ranked by NIH iCite citations
| Year | Title | Journal | Citations | PMID |
|---|---|---|---|---|
| 2012 | Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. | Cell | 1718 | 22658674 |
| 2002 | Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. | Proceedings of the National Academy of Sciences of the United States of America | 1479 | 12477932 |
| 2015 | The BioPlex Network: A Systematic Exploration of the Human Interactome. | Cell | 1118 | 26186194 |
| 2017 | Architecture of the human interactome defines protein communities and disease networks. | Nature | 1085 | 28514442 |
| 2014 | A proteome-scale map of the human interactome network. | Cell | 977 | 25416956 |
| 2020 | A reference map of the human binary protein interactome. | Nature | 849 | 32296183 |
| 2018 | VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. | Cell discovery | 829 | 29507755 |
| 1994 | The Vpr protein of human immunodeficiency virus type 1 influences nuclear localization of viral nucleic acids in nondividing host cells. | Proceedings of the National Academy of Sciences of the United States of America | 736 | 8041786 |
| 1993 | A nuclear localization signal within HIV-1 matrix protein that governs infection of non-dividing cells. | Nature | 708 | 8105392 |
| 2021 | Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. | Cell | 705 | 33961781 |
| 2012 | A census of human soluble protein complexes. | Cell | 689 | 22939629 |
| 1995 | Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence. | Nature | 660 | 7566098 |
| 2011 | Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. | Briefings in bioinformatics | 656 | 21873635 |
| 2006 | A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. | Cell | 610 | 16713569 |
| 2020 | Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms. | Science (New York, N.Y.) | 564 | 33060197 |
| 1992 | Active nuclear import of human immunodeficiency virus type 1 preintegration complexes. | Proceedings of the National Academy of Sciences of the United States of America | 513 | 1631159 |
| 2011 | The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. | Molecular and cellular biology | 437 | 21555454 |
| 2022 | OpenCell: Endogenous tagging for the cartography of human cellular organization. | Science (New York, N.Y.) | 432 | 35271311 |
| 1997 | HIV-1 infection of nondividing cells through the recognition of integrase by the importin/karyopherin pathway. | Proceedings of the National Academy of Sciences of the United States of America | 415 | 9275210 |
| 2015 | Panorama of ancient metazoan macromolecular complexes. | Nature | 407 | 26344197 |
| 2007 | Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. | Molecular cell | 367 | 17643375 |
| 2021 | A proximity-dependent biotinylation map of a human cell. | Nature | 339 | 34079125 |
| 2010 | Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics. | Cell | 318 | 21145461 |
| 1997 | HIV-1 infection of non-dividing cells: evidence that the amino-terminal basic region of the viral matrix protein is important for Gag processing but not for post-entry nuclear import. | The EMBO journal | 313 | 9303297 |
| 1995 | HIV nuclear import is governed by the phosphotyrosine-mediated binding of matrix to the core domain of integrase. | Cell | 312 | 7585960 |
| 1995 | HIV-1 infection of nondividing cells: C-terminal tyrosine phosphorylation of the viral matrix protein is a key regulator. | Cell | 288 | 7859280 |
| 1998 | Viral protein R regulates nuclear import of the HIV-1 pre-integration complex. | The EMBO journal | 275 | 9463369 |
| 1995 | Role of the basic domain of human immunodeficiency virus type 1 matrix in macrophage infection. | Journal of virology | 271 | 7745752 |
| 2017 | Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation. | Nature medicine | 251 | 28805822 |
| 1995 | Mutational analysis of cell cycle arrest, nuclear localization and virion packaging of human immunodeficiency virus type 1 Vpr. | Journal of virology | 245 | 7494303 |
| 2014 | Ebola virus VP24 targets a unique NLS binding site on karyopherin alpha 5 to selectively compete with nuclear import of phosphorylated STAT1. | Cell host & microbe | 187 | 25121748 |
| 2007 | An intracellular serpin regulates necrosis by inhibiting the induction and sequelae of lysosomal injury. | Cell | 133 | 17889653 |
| 2012 | Somatic mutations in the Notch, NF-KB, PIK3CA, and Hedgehog pathways in human breast cancers. | Genes, chromosomes & cancer | 63 | 22302350 |
| 2015 | Lloviu virus VP24 and VP35 proteins function as innate immune antagonists in human and bat cells. | Virology | 40 | 26255028 |
| 2017 | Macrocyclic peptide inhibitors for the protein-protein interaction of Zaire Ebola virus protein 24 and karyopherin alpha 5. | Organic & biomolecular chemistry | 39 | 28574091 |
| 2017 | VP24-Karyopherin Alpha Binding Affinities Differ between Ebolavirus Species, Influencing Interferon Inhibition and VP24 Stability. | Journal of virology | 33 | 27974555 |
| 2016 | Conserved differences in protein sequence determine the human pathogenicity of Ebolaviruses. | Scientific reports | 28 | 27009368 |
| 2022 | Lysoptosis is an evolutionarily conserved cell death pathway moderated by intracellular serpins. | Communications biology | 23 | 35022507 |
| 2015 | BIG3 Inhibits the Estrogen-Dependent Nuclear Translocation of PHB2 via Multiple Karyopherin-Alpha Proteins in Breast Cancer Cells. | PloS one | 23 | 26052702 |
| 2021 | Molecular profiling of nucleocytoplasmic transport factor genes in breast cancer. | Heliyon | 12 | 33553736 |
| 2023 | LncRNA SNHG6 Upregulates KPNA5 to Overcome Gemcitabine Resistance in Pancreatic Cancer via Sponging miR-944. | Pharmaceuticals (Basel, Switzerland) | 11 | 37259332 |
| 2021 | Effect of Ivermectin and Atorvastatin on Nuclear Localization of Importin Alpha and Drug Target Expression Profiling in Host Cells from Nasopharyngeal Swabs of SARS-CoV-2- Positive Patients. | Viruses | 9 | 34696514 |
| 2017 | An all-atom molecular dynamics study of the anti-interferon signaling of Ebola virus: interaction mechanisms of EBOV VP24 binding to Karyopherin alpha5. | Molecular bioSystems | 9 | 28418440 |
| 2017 | Investigating Ebola virus pathogenicity using molecular dynamics. | BMC genomics | 8 | 28812539 |
| 2018 | Ebolavirus interferon antagonists-protein interaction perspectives to combat pathogenesis. | Briefings in functional genomics | 7 | 29140409 |
| 2002 | Physical map of the chromosome 6q22 region containing the oculodentodigital dysplasia locus: analysis of thirteen candidate genes and identification of novel ESTs and DNA polymorphisms. | Cytogenetic and genome research | 5 | 12584438 |
| 2025 | MiR-153 Prevents NRF2 Nuclear Translocation to Drive Hypoperfusion-Related Cognitive Deficits by Targeting KPNA5. | The Journal of neuroscience : the official journal of the Society for Neuroscience | 4 | 40816766 |
| 2025 | Mesenchymal Stem Cell-Derived Exosome miR-153-3 Induced M2-Type Polarization of Macrophages to Improve the Healing Effect of Burn Wounds. | Applied biochemistry and biotechnology | 2 | 40053206 |
| 2025 | KPNA5 Suppresses Malignant Progression of Ovarian Cancer Through Importing the PTPN4 Into the Nucleus. | Cancer medicine | 2 | 40145330 |
| 2025 | The Biophysical Basis for Karyopherin-Dependent Ebola Virus VP24 Nuclear Transport. | Viruses | 1 | 40872766 |