Affinage

KLF7

Krueppel-like factor 7 · UniProt O75840

Length
302 aa
Mass
33.4 kDa
Annotated
2026-06-10
100 papers in source corpus 41 papers cited in narrative 41 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

KLF7 is a Krüppel-like zinc-finger transcription factor that binds CACCC motifs and KLF-type sites in target gene promoters and enhancers to activate or repress transcription, controlling cell-cycle exit, neuronal morphogenesis, lineage differentiation, and metabolic homeostasis (PMID:15964824, PMID:17123745, PMID:17553693). In neural and proliferative contexts it activates the CDK inhibitors p21/CDKN1A and p27/CDKN1B and represses cyclin D1 to enforce G1 arrest, a function central to its control of olfactory neuron differentiation and satellite-cell quiescence (PMID:15964824, PMID:11336497, PMID:26930448). During neuronal development it directly drives genes required for axon outgrowth and identity, including OMP and L1CAM, the neurotrophin receptor TrkA (acting synergistically with Brn3a) and TrkB (via the calcium/cAMP-responsive TCaRE3 element), and the chemorepellent Draxin, whose loss underlies hippocampal neurogenesis and granule-cell migration defects in conditional knockouts (PMID:17123745, PMID:17011544, PMID:17553693, PMID:40762575). KLF7 is broadly required for differentiation of neuroectodermal, mesodermal, and pluripotent lineages and can substitute for KLF4 in reprogramming to naive pluripotency (PMID:20580711, PMID:41094238). In the heart it simultaneously tunes glycolysis and fatty-acid oxidation by transactivating PFKL and ACADL, with cardiac-specific gain or loss producing distinct hypertrophic phenotypes (PMID:36810848). Across diverse cancers KLF7 acts as an oncogenic transcriptional driver, directly activating metabolic, signaling, and ECM-remodeling targets such as SLC1A5, ASL, PDGFB, ITGA2, LOX, IGF2BP2, and VPS35 to promote proliferation, metastasis, and stemness (PMID:32430335, PMID:31018905, PMID:33858520, PMID:39648156, PMID:38164176, PMID:40316546). KLF7 protein stability is controlled by SCF-Fbxw7-mediated polyubiquitylation that requires prior GSK-3-dependent phosphorylation of its Cdc4 phosphodegron, and its abundance is set upstream by transcriptional inputs including NF-κB/p65, NRF1, FOXO4, PU.1, and MLL1-deposited H3K4me3, while its co-activator MoKA binds KLF7 and stimulates its output (PMID:16990251, PMID:30838725, PMID:35443706, PMID:36647727, PMID:39007840, PMID:41231402, PMID:41483084).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2001 Medium

    Established that KLF7 is not merely a developmental marker but an active regulator of cell-cycle entry, linking it mechanistically to growth control.

    Evidence KLF7 overexpression in fibroblasts and neuroblastoma cells with cell-cycle analysis

    PMID:11336497

    Open questions at the time
    • Gain-of-function only, no direct promoter binding shown for cyclin D1 or p21
    • Single lab, no in vivo confirmation
  2. 2005 High

    Identified direct transcriptional targets (p21/CDKN1A, p27/CDKN1B) explaining how KLF7 enforces cell-cycle exit during neuronal differentiation in vivo.

    Evidence Klf7-/- mice, in situ hybridization, immunoblot, cotransfection/luciferase reporter assays

    PMID:15964824

    Open questions at the time
    • Whether p21/p27 activation is direct via defined CACCC sites not fully mapped
    • Tissue specificity of cofactor requirements unresolved
  3. 2006 High

    Demonstrated sequence-specific CACCC-motif binding to neuronal differentiation and adhesion gene promoters (OMP, L1CAM) and cooperative control of TrkA with Brn3a, establishing KLF7 as a direct transcriptional driver of neuronal identity and innervation.

    Evidence Microarray of Klf7-/- neurons, promoter transfection assays, Brn3a-/-;Klf7-/- double-knockout epistasis, IHC

    PMID:17011544 PMID:17123745

    Open questions at the time
    • Direct genome-wide binding not yet mapped at this stage
    • Mechanism of synergy with Brn3a at the molecular level unresolved
  4. 2007 High

    Defined a novel KLF7-bound regulatory element (TCaRE3) coupling KLF7 to activity-dependent (Ca2+/cAMP) TrkB transcription, connecting KLF7 to neuronal signal-responsive gene expression.

    Evidence EMSA for direct binding, TCaRE3 mutagenesis in luciferase reporters, Ca2+/cAMP stimulation

    PMID:17553693

    Open questions at the time
    • Signaling input that converts Ca2+/cAMP into KLF7 occupancy not defined
    • Post-translational modifications regulating TCaRE3 binding unknown
  5. 2006 Medium

    Identified MoKA as a physical KLF7 partner and co-activator with regulated nuclear-cytoplasmic shuttling, providing a mechanism for tuning KLF7 transcriptional output.

    Evidence Co-IP, NLS/NES fusion-protein expression, GAL4 chimeric reporter assays, leptomycin B treatment

    PMID:16990251

    Open questions at the time
    • Physiological contexts where MoKA shuttling controls KLF7 activity not established
    • Endogenous stoichiometry and reciprocal validation limited
  6. 2010 Medium

    Broadened KLF7 function beyond neurons by showing it is required for differentiation across neuroectodermal, cardiomyocytic, adipogenic, and osteogenic lineages.

    Evidence shRNA silencing in PC12 cells, Klf7-null neural stem cells, ESC differentiation assays, MEF lineage assays

    PMID:20580711

    Open questions at the time
    • Direct targets in each lineage not defined
    • Whether effects are cell-autonomous unresolved
  7. 2016 Medium

    Placed KLF7 downstream of TGF-β and Notch3 as a p21-dependent effector of satellite-cell quiescence, and implicated DNA-binding-domain acetylation (K227/K231) as a regulatory switch.

    Evidence KLF7 knockdown/overexpression in satellite cells, signaling epistasis, acetylation-mutant analysis

    PMID:26930448

    Open questions at the time
    • Acetyltransferase/deacetylase responsible for K227/K231 not identified
    • Direct effect of acetylation on DNA binding affinity not quantified
  8. 2019 High

    Defined the principal mechanism controlling KLF7 protein abundance: SCF-Fbxw7 polyubiquitylation gated by GSK-3 phosphorylation of a Cdc4 phosphodegron.

    Evidence DiPIUS proteomics, Co-IP, in vitro polyubiquitylation, CPD mutagenesis, GSK-3 inhibitor epistasis, half-life measurement

    PMID:30838725

    Open questions at the time
    • Signals that activate GSK-3-dependent CPD phosphorylation in vivo not defined
    • Whether degradation is regulated differentially across tissues unknown
  9. 2018 Medium

    Established KLF7 as a node in metabolic inflammation, transactivating IL-6 downstream of TLR4 in palmitic-acid-stimulated adipocytes.

    Evidence IL-6 promoter luciferase reporter, KLF7 and TLR4 manipulation, qRT-PCR, Western blot

    PMID:30598636

    Open questions at the time
    • Direct ChIP for KLF7 at IL-6 promoter not shown
    • Relationship to NF-κB occupancy unresolved
  10. 2022 Medium

    Identified upstream transcriptional control of KLF7 by NF-κB/p65 in lipotoxic adipose signaling, establishing a feed-forward inflammatory circuit.

    Evidence ChIP and luciferase reporter for p65 at KLF7 promoter, GPR40/GPR120 blockade in vivo and in vitro

    PMID:35443706

    Open questions at the time
    • Whether KLF7 reciprocally amplifies NF-κB in vivo not fully dissected
    • Tissue-specific dependence unresolved
  11. 2023 High

    Showed KLF7 sets cardiac substrate balance by directly co-regulating glycolysis (PFKL) and fatty-acid oxidation (ACADL), with dosage controlling hypertrophy phenotype.

    Evidence Cardiac-specific Klf7 knockout/overexpression mice, ChIP, luciferase reporter, PFKL/ACADL rescue

    PMID:36810848

    Open questions at the time
    • How a single factor coordinately tunes two opposing metabolic genes mechanistically unresolved
    • Developmental-stage specificity of opposite phenotypes not fully explained
  12. 2020 Medium

    Linked KLF7 to organelle integrity and tumor secretome, sustaining DLG3 to maintain Golgi structure, glycosylation, and chemokine secretion in PDAC.

    Evidence shRNA knockdown, xenograft, transcriptomics, glycosylation assays

    PMID:32430335

    Open questions at the time
    • Direct binding to DLG3 promoter not mapped
    • Mechanism connecting DLG3 to Golgi integrity unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • A large body of cancer studies establishes KLF7 as a context-dependent oncogenic transcription factor driving metabolic, signaling, ECM, and stemness programs, but a unifying determinant of its activator-versus-repressor behavior and cofactor selection across tissues remains undefined.
  • No structural model of KLF7 bound to DNA or cofactors
  • Rules governing activation versus repression of targets unknown
  • Whether the diverse cancer targets reflect distinct cofactor complexes is unresolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 7 GO:0003677 DNA binding 3
Localization
GO:0005634 nucleus 1
Pathway
R-HSA-1643685 Disease 6 R-HSA-1266738 Developmental Biology 4 R-HSA-1640170 Cell Cycle 4 R-HSA-74160 Gene expression (Transcription) 4 R-HSA-1430728 Metabolism 3 R-HSA-392499 Metabolism of proteins 1
Partners
BRN3AFBXW7FOXO4MOKANRF1PU.1

Evidence

Reading pass · 41 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2005 KLF7 transcriptionally activates the promoter of p21waf/cip (CDKN1A) in neuronal cells; loss of KLF7 in mice leads to significant downregulation of p21waf/cip and p27kip1 in olfactory epithelium, and cotransfection experiments demonstrated KLF7 can transactivate a reporter driven by the proximal p27kip1 promoter. Mouse knockout (Klf7-/-), in situ hybridization, immunoblot, cotransfection/luciferase reporter assay Molecular and cellular biology High 15964824
2001 Overexpression of KLF7 in cultured fibroblasts and neuroblastoma cells represses cyclin D1, activates p21, and leads to G1 growth arrest, indicating KLF7 regulates cell cycle entry. Overexpression in cell culture, cell cycle analysis Developmental biology Medium 11336497
2006 KLF7 directly binds CACCC motifs in the promoters of OMP (olfactory marker protein) and L1 (adhesion molecule) to activate their transcription in differentiating olfactory sensory neurons, as established by cell transfection and microarray profiling of Klf7-/- mice. DNA microarray (Klf7-/- vs wild-type olfactory neurons), cell transfection/promoter assays, CACCC-motif binding Gene Medium 17123745
2006 Brn3a and KLF7 synergistically activate the TrkA enhancer in vitro, and genetic double-knockout (Brn3a-/-;Klf7-/-) mice show severe reduction of TrkA expression at E12.5 and complete loss of Trk+ neurons at birth, demonstrating cooperative transcriptional regulation of TrkA. In vitro transcriptional synergy assay, double-mutant genetic epistasis (Brn3a-/-;Klf7-/- mice), immunohistochemistry Developmental biology High 17011544
2007 KLF7 binds to a novel regulatory element (TCaRE3) in the TRKB promoter in a sequence-specific manner (demonstrated by EMSA), and this element is required for Ca2+- and cAMP-stimulated TRKB transcription in neurons; mutations in TCaRE3 reduce basal expression by ≥80%. Electrophoretic mobility shift assay (EMSA), luciferase reporter with TCaRE3 mutations, Ca2+/cAMP stimulation assays Molecular and cellular neurosciences High 17553693
2006 MoKA, a novel F-box-containing protein, physically interacts with KLF7 and stimulates its transcriptional activity. MoKA nuclear localization is mediated by one specific NLS, while three separate sequences mediate cytoplasmic accumulation including one CRM1-dependent leucine-rich NES and two CRM1-independent export signals; the major activation domain of MoKA maps to a highly acidic sequence. Co-IP (MoKA–KLF7 interaction), forced expression of NLS/NES fusion proteins in mammalian cells, GAL4 chimeric transcriptional assays, leptomycin B (CRM1 inhibitor) treatment Nucleic acids research Medium 16990251
2010 KLF7 is required for differentiation of neuroectodermal and mesodermal cell lineages: shRNA-mediated silencing of KLF7 in PC12 cells downregulates MAP2 and TrkA; KLF7 inactivation in Klf7-null mice decreases BLBP/FABP7 in neural stem cells; Klf7 silencing impairs neuronal and cardiomyocytic differentiation of embryonic stem cells, and alters adipogenic and osteogenic potential of mouse embryonic fibroblasts. shRNA-mediated KLF7 silencing, Klf7-null mouse neural stem cells, embryonic stem cell differentiation assays, immunoblot Experimental cell research Medium 20580711
2016 KLF7 functions as a key mediator of TGF-β and Notch3 signaling-induced satellite cell quiescence and myoblast arrest; KLF7 is upregulated in quiescent satellite cells, and its knockdown promotes activation. The quiescence-promoting activity of KLF7 depends on p21 and on acetylation of Lys227 and/or Lys231 in the KLF7 DNA-binding domain. KLF7 knockdown/overexpression in satellite cells, epistasis with TGF-β and Notch3 signaling, acetylation mutant analysis (K227/K231) Stem cells Medium 26930448
2019 SCF-Fbxw7 ubiquitin ligase complex polyubiquitylates KLF7 for proteasomal degradation in a manner dependent on GSK-3-mediated phosphorylation of a Cdc4 phosphodegron (CPD) on KLF7; depletion of Fbxw7 stabilizes KLF7, and Fbxw7 overexpression downregulates the KLF7 target p21Cip1 in neuronal cells. DiPIUS proteomics screen, Co-IP (KLF7–Fbxw7 interaction), polyubiquitylation assay, CPD mutant analysis, GSK-3 inhibitor treatment, half-life measurement Genes to cells High 30838725
2018 KLF7 transcriptionally activates IL-6 by binding to its promoter region, acting downstream of TLR4 in palmitic acid (PA)-stimulated adipocytes; PA → TLR4 → KLF7 → NF-κB/IL-6 pathway established by luciferase reporter assay and siRNA knockdown experiments. Luciferase reporter assay (IL-6 promoter), KLF7 overexpression/knockdown, TLR4 manipulation, qRT-PCR, Western blot Mediators of inflammation Medium 30598636
2022 p65 (NF-κB subunit) transcriptionally upregulates KLF7 expression via direct binding to the KLF7 promoter in the palmitic acid/GPR40/GPR120-NF-κB pathway, demonstrated by luciferase reporter gene assay and ChIP assay. Luciferase reporter gene assay, chromatin immunoprecipitation (ChIP), GPR40/GPR120 blocker treatment in vivo and in vitro Nutrition & diabetes Medium 35443706
2020 KLF7 maintains Golgi complex integrity by transcriptionally sustaining DLG3 expression; KLF7 knockdown reduces DLG3, causing Golgi fragmentation, reduced protein glycosylation, and decreased secretion of chemokines that promote PDAC growth and metastasis. KLF7 also activates interferon-stimulated genes (ISGs) necessary for PDAC tumor growth. shRNA-mediated KLF7 knockdown, cell culture and xenograft mouse models, transcriptomic profiling, glycosylation assays Proceedings of the National Academy of Sciences of the United States of America Medium 32430335
2019 KLF7 transcriptionally activates argininosuccinate lyase (ASL), promoting polyamine biosynthesis (a urea cycle metabolite) in glioma cells; KLF7 knockdown reduces ASL expression and polyamine levels, and ASL overexpression rescues KLF7-driven glioma growth. Luciferase reporter assay (ASL promoter), KLF7 knockdown/overexpression, polyamine metabolite measurement, rescue experiments Biochemical and biophysical research communications Medium 31018905
2021 KLF7 transcriptionally activates VPS35, which then interacts with CCDC85C (confirmed by Co-IP), leading to activation of the β-catenin signaling pathway and promotion of HCC progression. ChIP-qPCR, luciferase reporter assay (VPS35 promoter), Co-IP (VPS35–CCDC85C), β-catenin inhibitor rescue, xenograft Cell & bioscience Medium 33858520
2023 KLF7 promotes HCC metastasis by directly transactivating TLR4 and PTK2; HMGB1 upregulates KLF7 via TLR4/RAGE-PI3K-AKT-NF-κB signaling, forming an HMGB1-KLF7-TLR4 positive feedback loop, confirmed by luciferase reporter and ChIP assays. Luciferase reporter assay, ChIP assay, orthotopic xenograft, DEN/CCl4 HCC models, genetic depletion of KLF7 Theranostics Medium 37554278
2023 KLF7 simultaneously transcriptionally targets PFKL (rate-limiting glycolysis enzyme) and ACADL (key fatty acid oxidation enzyme) in cardiomyocytes; cardiac-specific KLF7 knockout causes adult concentric hypertrophy and overexpression causes infant eccentric hypertrophy; knockdown of PFKL or overexpression of ACADL partially rescues hypertrophy in KLF7-deficient mice. Cardiac-specific KLF7 knockout/overexpression mice, ChIP assay, luciferase reporter, rescue experiments with PFKL knockdown/ACADL overexpression Nature communications High 36810848
2022 KLF7 binds the HDAC4 promoter to transcriptionally activate HDAC4; HDAC4 then reduces H3 and H4 acetylation at the miR-148b promoter to suppress miR-148b-3p, thereby promoting NCOR1 transcription and limiting glucose metabolic reprogramming in macrophages. Dual-luciferase assay, ChIP assay (KLF7 binding to HDAC4 promoter, HDAC4 at miR-148b promoter), HDAC4 knockdown rescue, AS mouse model Gerontology Medium 35439761
2023 KLF7 promotes IL-6 expression via the PKCζ/NF-κB pathway in adipocytes; luciferase reporter and ChIP assays confirmed that KLF7 transcriptionally upregulates PKCζ expression; adipocyte-specific KLF7 knockout mice showed decreased PKCζ, p-IκB, p-p65, and IL-6 in epididymal white adipose tissue. Fat-conditional KLF7 knockout mice, luciferase reporter assay, ChIP assay (KLF7 at PKCζ promoter), Western blot, qRT-PCR FASEB journal Medium 37342904
2024 KLF7 directly binds and transcriptionally activates the IGF2BP2 promoter and its super-enhancer region in head and neck squamous cell carcinoma, shown by ChIP-qPCR and dual-luciferase reporter assays; KLF7 abundance positively correlates with IGF2BP2 expression. H3K27Ac ChIP-seq, ChIP-qPCR, dual-luciferase reporter assay, CRISPR/Cas9 gene editing, small-molecule inhibitor (JQ1) Journal of experimental & clinical cancer research Medium 38443991
2022 KLF7 promotes preadipocyte proliferation by transcriptionally activating CDKN3 via a binding site upstream of the CDKN3 gene; CDKN3 in turn activates Akt signaling, driving the G1/S cell cycle transition (confirmed by ChIP-seq, promoter truncation/deletion analysis, and Akt phosphorylation assays). KLF7 ChIP-seq, luciferase reporter with 5'-truncation and binding-site deletion, CDKN3 knockdown/overexpression, flow cytometry (cell cycle), Akt phosphorylation assay Acta biochimica et biophysica Sinica Medium 36269137
2023 PU.1 directly binds KLF7 (confirmed by Co-IP and FRET) and enhances KLF7's transcriptional activation of CDKN3 in chicken preadipocytes; PU.1 overexpression inhibits preadipocyte differentiation and promotes proliferation. Co-immunoprecipitation, FRET assay, luciferase reporter (CDKN3 promoter), PU.1 overexpression/knockdown Acta biochimica et biophysica Sinica Medium 36647727
2017 KLF7 promotes the corneal progenitor cell state and antagonizes KLF4's corneal differentiation-promoting activity; KLF7 was shown by ChIP-seq to occupy corneal epithelial enhancers enriched for KLF motifs. ChIP-seq (KLF7 occupancy), KLF7 knockdown in primary human corneal epithelial cells, gene expression analysis The Journal of biological chemistry Medium 28916725
2024 KLF7 transcriptionally activates ALKBH5 by binding its promoter (confirmed by dual-luciferase and ChIP assays); ALKBH5 then reduces m6A modification of ACSL4 mRNA, decreasing ACSL4 protein and inhibiting ferroptosis in ox-LDL-treated endothelial cells. Dual-luciferase assay, ChIP assay, MeRIP assay for m6A modification of ACSL4, KLF7/ALKBH5 overexpression/knockdown, ferroptosis markers Cytotechnology Medium 39435423
2024 KLF7 directly binds and transcriptionally activates the SLC1A5 promoter in hepatocellular carcinoma cells, promoting tryptophan uptake and serotonin biosynthesis; increasing serotonin restores malignancy in KLF7-knockdown cells. ChIP assay (KLF7 at SLC1A5 promoter), KLF7 knockdown/overexpression, serotonin measurement, serotonin rescue experiment Journal of cellular and molecular medicine Medium 39648156
2021 GNA14 stimulates KLF7 expression in endometrial cancer cells, and KLF7 transcriptionally activates HAS2 (hyaluronan synthase 2); KLF7/HAS2 axis promotes UCEC cell proliferation and migration, with HAS2 knockdown reversing KLF7-driven oncogenic effects. GNA14/KLF7 knockdown/overexpression, RNA sequencing, qRT-PCR, Western blot, rescue experiments (HAS2 knockdown), xenograft BMC cancer Low 33892667
2024 KLF7 directly binds the promoter of PDGFB (at TGGGTGGAG motif), driving PDGFB transcription and secretion; secreted PDGFB activates MAPK/ERK, PI3K/AKT, and JAK/STAT3 pathways through PDGFRβ to promote colon adenocarcinoma progression. ChIP assay (KLF7 at PDGFB promoter), luciferase reporter, KLF7 knockdown/overexpression, in vitro and in vivo experiments, sunitinib pharmacological blockade International journal of biological sciences Medium 38164176
2022 KLF7 directly binds the MKNK2 promoter to repress MKNK2 transcription (confirmed by ChIP and dual-luciferase assays), thereby suppressing HIF-1 signaling and M1 microglia polarization after ischemic stroke. ChIP assay, dual-luciferase reporter, KLF7 overexpression/knockdown, MCAO/R rat model, MKNK2 knockdown/overexpression Brain and behavior Medium 40923147
2024 NRF1 binds to the KLF7 promoter to enhance KLF7 transcription; USP7 stabilizes NRF1 via deubiquitination; collectively USP7→NRF1→KLF7 axis protects neurons from inflammation and apoptosis after spinal cord injury (confirmed by Co-IP, ChIP, and dual-luciferase assays). Co-immunoprecipitation (USP7–NRF1), ChIP assay (NRF1 at KLF7 promoter), dual-luciferase reporter, KLF7/NRF1/USP7 knockdown/overexpression, SCI rat and LPS cell models Neurological research Medium 39007840
2025 KLF7 directly binds the promoters of AHNAK, AHNAK2, and GDPD5, regulating their expression and altering GTPase activity to induce neuroblastoma cell differentiation; KLF7 acts independently of the retinoic acid pathway and cooperatively with RA; silencing KLF7 promotes adrenergic-to-mesenchymal transition. ChIP assay (KLF7 at AHNAK/AHNAK2/GDPD5 promoters), KLF7 knockdown/overexpression, GTPase activity assay, RA co-treatment experiments, transcriptomic profiling The FEBS journal Medium 38924469
2025 KLF7 deletion in hippocampal progenitors (Emx1-Cre;Klf7F/F mice) causes hippocampal shrinkage with disrupted neurogenesis, neuronal differentiation, and migration; transcriptomic profiling identified Draxin (a neural chemorepellent) as a direct downstream KLF7 target, and overexpression of Draxin rescued dentate gyrus granule cell migration defects in KLF7 mutant mice. Conditional knockout mice (Emx1-Cre;Klf7F/F), RNA-seq transcriptomic profiling, Draxin overexpression rescue, behavioral assays (anxiety, memory) Development (Cambridge, England) High 40762575
2025 FOXO4 transcriptionally activates KLF7 by binding its promoter (confirmed by ChIP-qPCR and dual-luciferase assay); KLF7 in turn activates the TLR4/MyD88/NF-κB pathway to promote high-glucose-induced retinal pigment epithelial cell injury. ChIP-qPCR, dual-luciferase reporter (FOXO4 at KLF7 promoter), FOXO4/KLF7 knockdown/overexpression, NF-κB inhibitor (BAY 11-7085) epistasis, HG-challenged RPE cells Applied biochemistry and biotechnology Medium 41231402
2025 MLL1 promotes KLF7 transcription through H3K4me3 modification at the KLF7 promoter in rheumatoid arthritis fibroblast-like synoviocytes; KLF7 then transcriptionally activates USP7; this MLL1/KLF7/USP7 axis promotes FLS proliferation and invasion. ChIP assay (MLL1 and H3K4me3 at KLF7 promoter; KLF7 at USP7 promoter), dual-luciferase assay, shRNA knockdown, CCK-8/Transwell assays In vitro cellular & developmental biology. Animal Medium 41483084
2024 KLF7 directly binds and transcriptionally activates the ITGA2 promoter and super-enhancer in oral squamous cell carcinoma (confirmed by ChIP-seq and dual-luciferase assays); ITGA2, upon binding its ECM ligand type I collagen, activates PI3K-AKT, MAPK, and Hippo signaling to maintain cancer stem cell stemness. ChIP-seq (KLF7 at ITGA2 locus), dual-luciferase assay, ITGA2 knockdown, tumor sphere formation, flow cytometry, limiting dilution xenograft assay, TC-I 15 inhibitor treatment Cell death & disease Medium 40316546
2025 KLF7 transcriptionally activates LOX (lysyl oxidase) in HNSCC cells (direct transcriptional activation), and LOX-driven ECM crosslinking stiffens the tumor microenvironment to recruit macrophages and suppress CD8+ T cell killing. KLF7 knockdown/overexpression, in vitro and in vivo (xenograft) experiments, LOX identified as direct KLF7 transcriptional target (described as 'bona fide target'), macrophage recruitment assays Cancer letters Low 41482204
2024 KLF7 transcriptionally activates LIMK1 by binding its promoter (confirmed by ChIP and dual-luciferase assays); LIMK1 then physically interacts with SRPK1 (confirmed by GST pull-down and Co-IP) to promote SRPK1 phosphorylation, driving inflammation in LPS-treated alveolar epithelial cells. ChIP assay, dual-luciferase assay (KLF7 at LIMK1 promoter), GST pull-down and Co-IP (LIMK1–SRPK1 interaction), KLF7/LIMK1/SRPK1 knockdown/overexpression, ELISA Central-European journal of immunology Medium 42028385
2026 KLF7 directly binds the PPP1R14C promoter to drive its transcription; PPP1R14C physically interacts with and inhibits the PP1 catalytic subunit, sustaining CDK1 hyperactivation and promoting lung squamous cell carcinoma proliferation and invasion. ChIP assay (KLF7 at PPP1R14C promoter), Co-IP (PPP1R14C–PP1), KLF7/PPP1R14C overexpression/knockdown, CDK1 pharmacological inhibition (rescue), in vivo tumorigenesis Scientific reports Medium 41699008
2025 KLF7 promotes colorectal cancer liver metastasis by transcriptionally activating TGFβ autocrine signaling, driving EMT; EGCG inhibits KLF7 transcriptional activity and suppresses TGFβ/EMT (confirmed by dual-luciferase reporter assays and in vitro/in vivo experiments). Dual-luciferase reporter assay, KLF7 knockdown/overexpression, in vitro and in vivo metastasis assays, EGCG inhibitor treatment Cancer letters Low 41213461
2025 KLF7 is highly expressed in conventional and naive human pluripotent stem cells and can replace KLF4 in OSKM somatic reprogramming; forced KLF7 expression induces upregulation of naive pluripotency markers and enables chemical resetting to naive PSCs; CRISPRi-mediated KLF7 silencing reduces efficiency of chemical resetting without affecting maintenance of conventional PSCs. KLF7 overexpression replacing KLF4 in OSKM reprogramming, CRISPRi-mediated KLF7 silencing, transcriptome analysis, naive/primed PSC culture and chemical resetting assays EMBO reports Medium 41094238
2025 UKLF/KLF7 transcriptionally activates SLC39A4 expression; PCBP2 binds the 3'-UTR of UKLF mRNA to enhance its stability; together the PCBP2/UKLF/SLC39A4 pathway promotes colorectal cancer progression. ChIP assay/luciferase assay (KLF7 at SLC39A4 promoter), RNA-binding protein assay (PCBP2 binding to UKLF 3'-UTR), KLF7/PCBP2/SLC39A4 knockdown, xenograft Biochimica et biophysica acta. Molecular cell research Medium 38768927
2025 KLF7 transcriptionally represses MKNK2 and promotes PDE4 repression: in PCOS granulosa cells, KLF7 binds the PDE4 promoter and suppresses PDE4 transcription (confirmed by ChIP and dual-luciferase assay); KLF7 overexpression promotes granulosa cell proliferation and inhibits apoptosis, and PDE4 overexpression reverses these effects. ChIP assay, dual-luciferase reporter (KLF7 at PDE4 promoter), KLF7/PDE4 overexpression, CCK-8, TUNEL, EdU assays in DHT-treated HGL5 cells Endocrine connections Medium 41071839
2026 In hypopharyngeal squamous cell carcinoma, HDAC6 removes H3K9ac marks at the KLF7 promoter to suppress KLF7 expression; when HDAC6 is downregulated in HPSCC, KLF7 is upregulated and transcriptionally induces THBS1, which activates p38 MAPK signaling to drive EMT and lung metastasis. ChIP assay (H3K9ac at KLF7 promoter; KLF7 at THBS1 promoter), HDAC6/KLF7/THBS1 lentiviral knockdown/overexpression, EdU/colony formation/Transwell assays, in vivo lung metastasis (tail-vein injection), Western blot for EMT markers iScience Medium 41732256

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Transcription factor KLF7 is important for neuronal morphogenesis in selected regions of the nervous system. Molecular and cellular biology 128 15964824
2001 Developmental expression of mouse Krüppel-like transcription factor KLF7 suggests a potential role in neurogenesis. Developmental biology 91 11336497
2020 KLF7 promotes pancreatic cancer growth and metastasis by up-regulating ISG expression and maintaining Golgi complex integrity. Proceedings of the National Academy of Sciences of the United States of America 67 32430335
2013 Klf7 modulates the differentiation and proliferation of chicken preadipocyte. Acta biochimica et biophysica Sinica 56 23439665
2022 Obesity-induced elevated palmitic acid promotes inflammation and glucose metabolism disorders through GPRs/NF-κB/KLF7 pathway. Nutrition & diabetes 54 35443706
2018 miR-185 Inhibits the Proliferation and Invasion of Non-Small Cell Lung Cancer by Targeting KLF7. Oncology research 46 29716672
2023 HMGB1-mediated elevation of KLF7 facilitates hepatocellular carcinoma progression and metastasis through upregulating TLR4 and PTK2. Theranostics 45 37554278
2021 microRNA-204 shuttled by mesenchymal stem cell-derived exosomes inhibits the migration and invasion of non-small-cell lung cancer cells via the KLF7/AKT/HIF-1α axis. Neoplasma 45 33847133
2010 Transcription factor KLF7 regulates differentiation of neuroectodermal and mesodermal cell lineages. Experimental cell research 43 20580711
2006 Identification of genes regulated by transcription factor KLF7 in differentiating olfactory sensory neurons. Gene 43 17123745
2019 STAT3-induced long noncoding RNA LINC00668 promotes migration and invasion of non-small cell lung cancer via the miR-193a/KLF7 axis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 41 31150989
2023 The KLF7/PFKL/ACADL axis modulates cardiac metabolic remodelling during cardiac hypertrophy in male mice. Nature communications 39 36810848
2018 The Effect and Mechanism of KLF7 in the TLR4/NF-κB/IL-6 Inflammatory Signal Pathway of Adipocytes. Mediators of inflammation 39 30598636
2017 Correlation of TLR4 and KLF7 in Inflammation Induced by Obesity. Inflammation 37 27714571
2016 KLF7-transfected Schwann cell graft transplantation promotes sciatic nerve regeneration. Neuroscience 36 27826105
2020 KLF7: a new candidate biomarker and therapeutic target for high-grade serous ovarian cancer. Journal of experimental & clinical cancer research : CR 35 33250051
2019 KLF7 promotes polyamine biosynthesis and glioma development through transcriptionally activating ASL. Biochemical and biophysical research communications 35 31018905
2006 Brn3a and Klf7 cooperate to control TrkA expression in sensory neurons. Developmental biology 34 17011544
2021 KLF7/VPS35 axis contributes to hepatocellular carcinoma progression through CCDC85C-activated β-catenin pathway. Cell & bioscience 32 33858520
2024 KLF7 regulates super-enhancer-driven IGF2BP2 overexpression to promote the progression of head and neck squamous cell carcinoma. Journal of experimental & clinical cancer research : CR 31 38443991
2017 KLF7 overexpression in human oral squamous cell carcinoma promotes migration and epithelial-mesenchymal transition. Oncology letters 30 28454392
2006 Mice without transcription factor KLF7 provide new insight into olfactory bulb development. Brain research 30 16814267
2018 Inhibition of KLF7-Targeting MicroRNA 146b Promotes Sciatic Nerve Regeneration. Neuroscience bulletin 29 29356943
2016 KLF7 Regulates Satellite Cell Quiescence in Response to Extracellular Signaling. Stem cells (Dayton, Ohio) 29 26930448
2016 Combined chondroitinase and KLF7 expression reduce net retraction of sensory and CST axons from sites of spinal injury. Neurobiology of disease 26 27988344
2017 Characterization of enhancers and the role of the transcription factor KLF7 in regulating corneal epithelial differentiation. The Journal of biological chemistry 25 28916725
2020 miR-450b-3p inhibited the proliferation of gastric cancer via regulating KLF7. Cancer cell international 23 32063748
2019 KLF7 overexpression in bone marrow stromal stem cells graft transplantation promotes sciatic nerve regeneration. Journal of neural engineering 23 31296795
2024 Hypoxic preconditioning-engineered bone marrow mesenchymal stem cell-derived exosomes promote muscle satellite cell activation and skeletal muscle regeneration via the miR-210-3p/KLF7 mechanism. International immunopharmacology 22 39306891
2020 MicroRNA‑103 modulates tumor progression by targeting KLF7 in non‑small cell lung cancer. International journal of molecular medicine 22 32582959
2022 KLF7 Alleviates Atherosclerotic Lesions and Inhibits Glucose Metabolic Reprogramming in Macrophages by Regulating HDAC4/miR-148b-3p/NCOR1. Gerontology 20 35439761
2024 Cytotoxic and epigenetic effects of berberine-loaded chitosan/pectin nanoparticles on AGS gastric cancer cells: Role of the miR-185-5p/KLF7 axis, DNMTs, and global DNA methylation. International journal of biological macromolecules 18 38253156
2020 MicroRNA-136-3p inhibits glioma tumorigenesis in vitro and in vivo by targeting KLF7. World journal of surgical oncology 18 32677950
2007 Ca2+, CREB and krüppel: a novel KLF7-binding element conserved in mouse and human TRKB promoters is required for CREB-dependent transcription. Molecular and cellular neurosciences 18 17553693
2023 KLF7 promotes adipocyte inflammation and glucose metabolism disorder by activating the PKCζ/NF-κB pathway. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 17 37342904
2022 LncRNA NUTM2A-AS1 aggravates the progression of hepatocellular carcinoma by activating the miR-186-5p/KLF7-mediated Wnt/beta-catenin pathway. Human cell 17 36242728
2021 MicroRNA-301a-3p increases oxidative stress, inflammation and apoptosis in ox-LDL-induced HUVECs by targeting KLF7. Experimental and therapeutic medicine 17 33850541
2016 Sciatic nerve regeneration in KLF7-transfected acellular nerve allografts. Neurological research 17 27093235
2023 Promotion of colorectal cancer by transcription factor BHLHE40 involves upregulation of ADAM19 and KLF7. Frontiers in oncology 16 36890812
2022 Transcription Factor KLF7 Promotes Osteoclast Differentiation by Suppressing HO-1. Frontiers in genetics 16 35419025
2021 Methylation of miR-19b-3p promoter exacerbates inflammatory responses in sepsis-induced ALI via targeting KLF7. Cell biology international 16 33760339
2023 miR-132-3p and KLF7 as novel regulators of aortic stiffening-associated EndMT in type 2 diabetes mellitus. Diabetology & metabolic syndrome 15 36698180
2020 Long noncoding RNA KCNQ1OT1 contributes to tumor growth and activates Wnt/β‑catenin signaling in osteosarcoma by targeting the miR‑3666/KLF7 axis. International journal of molecular medicine 15 33416089
2018 De novo variants in KLF7 are a potential novel cause of developmental delay/intellectual disability, neuromuscular and psychiatric symptoms. Clinical genetics 14 29251763
2006 Multiple pathways regulate intracellular shuttling of MoKA, a co-activator of transcription factor KLF7. Nucleic acids research 14 16990251
2022 Long noncoding RNA PVT1 regulates the proliferation and apoptosis of ARPE-19 cells in vitro via the miR-1301-3p/KLF7 axis. Cell cycle (Georgetown, Tex.) 12 35451342
2021 GNA14 stimulation of KLF7 promotes malignant growth of endometrial cancer through upregulation of HAS2. BMC cancer 12 33892667
2021 LncRNA SNHG14 accelerates breast cancer progression through sponging miR-543 and regulating KLF7 expression. Archives of gynecology and obstetrics 12 34783894
2020 KLF7 promotes macrophage activation by activating the NF-κB signaling pathway in epicardial adipose tissue in patients with coronary artery disease. European review for medical and pharmacological sciences 12 32633394
2021 KLF7 Promotes Gastric Carcinogenesis Through Regulation of ANTXR1. Cancer management and research 11 34285576
2019 SCFFbxw7 ubiquitylates KLF7 for degradation in a manner dependent on GSK-3-mediated phosphorylation. Genes to cells : devoted to molecular & cellular mechanisms 11 30838725
2023 The active phthalate metabolite, DHEP, induces proliferation and metastasis of prostate cancer cells via upregulation of β-catenin and downregulation of KLF7. Bioorganic chemistry 10 37734194
2022 KLF7 promotes preadipocyte proliferation via activation of the Akt signaling pathway by Cis-regulating CDKN3. Acta biochimica et biophysica Sinica 10 36269137
2024 UKLF/PCBP2 axis governs the colorectal cancer development by transcriptionally activating SLC39A4. Biochimica et biophysica acta. Molecular cell research 8 38768927
2023 Circular hsa_circ_0020377 regulates KLF7 by targeting miR-194-5p to facilitate tumor cell malignant behaviors and glycolysis in oral squamous cell carcinoma progression. Functional & integrative genomics 8 36717528
2021 Effects of miR-132-3p on progress and epithelial mesenchymal transition of non-small cell lung cancer via regulating KLF7. Journal of thoracic disease 8 34012590
2020 DNA methylation of CpG sites in the chicken KLF7 promoter and Exon 2 in association with mRNA expression in abdominal adipose tissue and blood metabolic indicators. BMC genetics 8 33054719
2025 KLF7-regulated ITGA2 as a therapeutic target for inhibiting oral cancer stem cells. Cell death & disease 7 40316546
2024 miR-520f-3p blocks MNNG-induced gastric precancerous lesions via the KLF7/NFκB pathway. Toxicology letters 7 38184286
2024 Palmitic acid-activated GPRs/KLF7/CCL2 pathway is involved in the crosstalk between bone marrow adipocytes and prostate cancer. BMC cancer 7 38221626
2024 KLF7 Promotes Hepatocellular Carcinoma Progression Through Regulating SLC1A5-Mediated Tryptophan Metabolism. Journal of cellular and molecular medicine 7 39648156
2022 Notoginsenoside R1 alleviates spinal cord injury through the miR-301a/KLF7 axis to activate Wnt/β-catenin pathway. Open medicine (Warsaw, Poland) 7 35509687
2014 Luna, a Drosophila KLF6/KLF7, is maternally required for synchronized nuclear and centrosome cycles in the preblastoderm embryo. PloS one 7 24915236
2024 USP7 alleviates neuronal inflammation and apoptosis in spinal cord injury via deubiquitinating NRF1/KLF7 axis. Neurological research 6 39007840
2024 KLF7 enhances the invasion and migration of colorectal cancer cells via the miR-139-5p/TPD52 axis. Cancer biology & therapy 6 39097779
2024 KLF7 reverses ox-LDL-induced ferroptosis in HMEC-1 cells through transcriptionally activating ALKBH5 to inhibit the m6A modification of ACSL4. Cytotechnology 6 39435423
2024 Alpha-lipoic acid targets KLF7 expression to inhibit cervical cancer progression. Acta biochimica et biophysica Sinica 6 39696984
2024 KLF7 promotes colon adenocarcinoma progression through the PDGFB signaling pathway. International journal of biological sciences 5 38164176
2024 KLF7 promotes neuroblastoma differentiation through the GTPase signaling pathway by upregulating neuroblast differentiation-associated protein AHNAKs and glycerophosphodiesterase GDPD5. The FEBS journal 5 38924469
2023 Stress-inducible IL-6 is regulated by KLF7 in brown adipocytes. Heliyon 5 37025783
2022 Long non-coding RNA XIST negatively regulates thoracic aortic aneurysm cell proliferation by targeting the miR-193a-5p/KLF7 axis. Cellular and molecular biology (Noisy-le-Grand, France) 5 36495497
2021 MiR-1b up-regulation inhibits rat neuron proliferation and regeneration yet promotes apoptosis via targeting KLF7. Folia neuropathologica 5 33969678
2025 KLF7 promotes progression of Head and Neck Squamous Cell Carcinoma by remodeling tumor immune microenvironment. Cancer letters 4 41482204
2024 Newborn adiposity is associated with cord blood DNA methylation at IGF1R and KLF7. Obesity (Silver Spring, Md.) 4 39165088
2021 Transcriptional control of chicken KLF7 promoter in preadipocytes. Acta biochimica et biophysica Sinica 4 33330912
2021 Elevation of LncRNA ENST00000453774.1 Prevents Renal Fibrosis by Upregulating FBN1, IGF1R, and KLF7. Kidney & blood pressure research 4 34614499
2023 Analysis of KLF7 and KLF5 transcription factors gene variants in coronary artery disease. Revista portuguesa de cardiologia : orgao oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology 3 37268267
2022 Expression and Clinical Significance of Serum Krüppel-Like Factor 7 (KLF7) in NSCLC Patients. Computational and mathematical methods in medicine 3 35936369
2021 A Missense Mutation in the KLF7 Gene Is a Potential Candidate Variant for Congenital Deafness in Australian Stumpy Tail Cattle Dogs. Genes 3 33805165
2025 KLF7 Blocks MKNK2/HIF-1 Pathway-Mediated M1 Microglia Polarization to Ameliorate Ischemic Stroke-Induced Neurological Injury. Brain and behavior 2 40923147
2023 The Oncogenic Role of KLF7 in Colon Adenocarcinoma and Therapeutic Perspectives. International journal of genomics 2 38116138
2022 Cancer-suppressing miR-520-3p gene inhibits proliferation, migration, and invasion of gastric cancer cells through targeted regulation of KLF7. Bulletin du cancer 2 35465967
2022 Inhibition of microRNA-19a-3p alleviates the neuropathic pain (NP) in rats after chronic constriction injury (CCI) via targeting KLF7. Transplant immunology 2 36334791
2021 Knockdown of KLF7 inhibits the differentiation of both intramuscular and subcutaneous preadipocytes in goat. Animal biotechnology 2 34890305
2025 KLF7 orchestrates hippocampal development through neurogenesis and Draxin-mediated neuronal migration. Development (Cambridge, England) 1 40762575
2025 KLF7-IT1-ARPC2 axis in macrophages orchestrates caspase-4-driven hypercoagulation in sepsis. Biochemical and biophysical research communications 1 41275797
2026 MLL1-pioneered H3K4me3 modification on KLF7 promoter accelerates the invasion of fibroblast-like synoviocytes in rheumatoid arthritis. In vitro cellular & developmental biology. Animal 0 41483084
2026 KLF7 induced ADRB3-dependent IL-6 production in brown adipocytes during stress. Journal of lipid research 0 41565113
2026 Transcriptional activation of PPP1R14C by KLF7 unleashes CDK1 activity to promote lung squamous cell carcinoma. Scientific reports 0 41699008
2026 THBS1 upregulation by KLF7 in hypopharyngeal squamous cell carcinoma contributes to lung metastasis through the p38 MAPK signaling. iScience 0 41732256
2026 In silico and in vitro analysis: Unveiling the therapeutic potential of flavonoids against KLF7 in ovarian cancer. Biochemical and biophysical research communications 0 41930827
2025 circ_0000389 inhibits intervertebral disc degeneration by targeting the miR-346/KLF7 axis. Acta biochimica et biophysica Sinica 0 40055913
2025 Mechanism of KLF7 in regulating the proliferation and apoptosis of granulosa cells in polycystic ovarian syndrome. Endocrine connections 0 41071839
2025 KLF7 is a general inducer of human pluripotency. EMBO reports 0 41094238
2025 KLF7 as a biomarker for the pre-metastatic state promotes colorectal cancer liver metastasis via TGFβ autocrine signaling. Cancer letters 0 41213461
2025 FOXO4 Facilitates Diabetic Retinopathy by Mediating KLF7 Transcription and Affecting the TLR4/MyD88/NF-κB Pathway. Applied biochemistry and biotechnology 0 41231402
2025 KLF7 enhances the inflammatory response in LPS-induced alveolar epithelial cells via activating the LIMK1/SRPK1 pathway. Central-European journal of immunology 0 42028385
2024 To explore the protective effect and mechanism of KLF7 overexpression on retinal ganglion cells after optic nerve crush in mice. European journal of ophthalmology 0 39513284
2023 PU.1 interacts with KLF7 to suppress differentiation and promote proliferation in chicken preadipocytes. Acta biochimica et biophysica Sinica 0 36647727
2023 [Overexpression of chicken Klf2 promotes klf7 transcription and inhibits adipocyte differentiation]. Sheng wu gong cheng xue bao = Chinese journal of biotechnology 0 37154331

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