Affinage

KAT2B

Histone acetyltransferase KAT2B · UniProt Q92831

Length
832 aa
Mass
93.0 kDa
Annotated
2026-06-10
100 papers in source corpus 56 papers cited in narrative 55 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

KAT2B (PCAF) is a GNAT-family lysine acetyltransferase that functions as a transcriptional co-activator and broad-spectrum protein-modifying enzyme, coupling chromatin acetylation to the control of cell differentiation, cell-cycle progression, genome maintenance, and metabolism (PMID:9742083, PMID:11009610, PMID:9659901). Mechanistically it catalyzes acetyl transfer through a fully ordered Bi-Bi mechanism in which acetyl-CoA binds first and acetyl transfer to histone H3 Lys-14 is rate-determining, with Glu-570 acting as the proposed general base; its extended N-terminal domain (relative to yeast Gcn5p) enables acetylation of nucleosomal as well as free histones (PMID:11009610, PMID:9742083). On chromatin, KAT2B deposits H3K9/H3K14 acetylation cooperatively with p300/CBP and within larger machines, including a cdk8-containing Mediator complex where H3 Ser-10 phosphorylation stimulates KAT2B-mediated H3K14 acetylation to produce activating tandem phosphoacetylation (PMID:18418385, PMID:9742083). Through direct interactions it serves as a co-activator for diverse transcription factors and signaling effectors—MyoD-driven myogenesis and p21 induction, nuclear receptors, TGF-β/Smad3, Notch/RBP-J, p73, and GLI1—frequently requiring its intrinsic HAT activity (PMID:9659901, PMID:9620851, PMID:11058129, PMID:10747963, PMID:14614455, PMID:23943798). Beyond histones, KAT2B acetylates a wide range of non-histone substrates to alter their activity, stability, or localization: p53 at K320 to enhance DNA binding after damage (PMID:9891054), PTEN at K125/K128 to dampen its lipid-phosphatase activity and sustain PI3K/AKT signaling (PMID:16829519), β-catenin at ubiquitination sites K19/K49 to block its degradation (PMID:18987336), EZH2 at K348 to stabilize it (PMID:25800736), cdk2 at K33 and p27 at K100 to restrain cell-cycle kinases (PMID:19773423, PMID:22547391), PLK4 at K45/K46 to limit centrosome amplification (PMID:27796307), PGC-1α to trigger its degradation and suppress gluconeogenesis (PMID:25497092), and RPA1 at K163 to drive nucleotide excision repair (PMID:28854354). KAT2B also carries a mechanistically distinct intrinsic E3 ubiquitin ligase activity that ubiquitinates Hdm2 (stabilizing p53) and GLI1 (terminating Hedgehog signaling) (PMID:17293853, PMID:24013724). At the genome it acts at stalled replication forks, where it acetylates H4K8 to recruit MRE11/EXO1 and promote fork degradation, an activity restrained by ATR phosphorylation at S264 (PMID:32966758). Its own nuclear localization is governed by autoacetylation of a C-terminal NLS, which is reversed by HDAC3/SIRT1 to drive cytoplasmic accumulation (PMID:19015268, PMID:12888487), and a cytoplasmic, HAT-independent pool inhibits the kinase TBK1 to negatively regulate IFN-β production (PMID:25269644). KAT2B is functionally redundant with GCN5, which is upregulated in PCAF-null mice to compensate (PMID:11027331).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1998 High

    Established that human PCAF/GCN5 differ from yeast Gcn5p by acetylating nucleosomal substrates, defining KAT2B as a bona fide chromatin-modifying enzyme that partners with CBP/p300.

    Evidence In vitro HAT assays on free versus nucleosomal histones with comparative domain analysis

    PMID:9742083

    Open questions at the time
    • Did not resolve which complexes target it to chromatin in vivo
    • Substrate site specificity on nucleosomes not fully mapped
  2. 2000 High

    Defined the catalytic mechanism, showing KAT2B uses an ordered Bi-Bi mechanism with acetyl-CoA binding first and acetyl transfer as the rate-limiting chemical step.

    Evidence Bi-substrate kinetics, product inhibition, equilibrium dialysis, and pre-steady-state quench-flow on H3 peptide

    PMID:11009610

    Open questions at the time
    • Kinetics established on peptide, not full nucleosomes
    • General-base role of Glu-570 inferred, not structurally proven in this study
  3. 1997 High

    Showed KAT2B HAT activity is specifically required for terminal differentiation, linking its enzymatic function to p21 induction and cell-cycle exit in myogenesis.

    Evidence Anti-PCAF antibody microinjection, HAT-dead mutant, and reporter assays in muscle differentiation

    PMID:9659901

    Open questions at the time
    • Direct substrates driving p21 induction not pinned to histone vs non-histone
    • Did not separate PCAF from GCN5 contribution
  4. 1999 High

    Identified the first non-histone substrate, p53 at K320, showing acetylation enhances p53 DNA binding and is induced by DNA damage—extending KAT2B function beyond chromatin.

    Evidence In vitro acetyltransferase assay with site-specific antibodies and in vivo detection after genotoxic stress

    PMID:9891054

    Open questions at the time
    • Erasers of K320 not identified here
    • In vivo functional consequence for specific target genes not resolved
  5. 1999 High

    Demonstrated that viral and cellular antagonists directly inhibit KAT2B, establishing it as a regulated node; E1A, E1B-55K, and MDM2 bind PCAF and block its acetylation of p53.

    Evidence Direct binding and in vitro/in vivo HAT inhibition assays for E1A, E1B-55K, and MDM2

    PMID:10025405 PMID:10891493 PMID:12068014

    Open questions at the time
    • Structural basis of inhibition not fully resolved
    • Whether inhibition is global or substrate-selective varies by antagonist
  6. 2000 High

    Genetically established functional redundancy between KAT2B and GCN5, explaining the viability of PCAF-null animals.

    Evidence PCAF-knockout mouse with Western quantification of compensatory GCN5 upregulation

    PMID:11027331

    Open questions at the time
    • Did not identify non-redundant KAT2B-specific functions in vivo
    • Tissue-specific requirements not dissected
  7. 2003 Medium

    Showed KAT2B activity and localization are self-regulated by autoacetylation, with p300 also acetylating it and autoacetylation boosting HAT activity.

    Evidence In vitro and in vivo acetylation assays with NLS-lysine mutagenesis

    PMID:12888487

    Open questions at the time
    • Single lab without independent replication
    • Quantitative contribution of inter- vs intramolecular autoacetylation unclear
  8. 2008 Medium

    Connected autoacetylation to subcellular control, showing NLS acetylation drives nuclear retention while HDAC3/SIRT1 deacetylation causes cytoplasmic accumulation.

    Evidence Subcellular fractionation, imaging, HAT-dead mutants, and HDAC manipulation

    PMID:19015268

    Open questions at the time
    • Signals triggering deacetylation-driven export not defined
    • Single lab
  9. 2007 High

    Revealed a second, mechanistically distinct enzymatic activity: an intrinsic E3 ubiquitin ligase that ubiquitinates Hdm2 to stabilize p53.

    Evidence In vitro ubiquitination assay plus knockdown phenotype with domain deletion

    PMID:17293853

    Open questions at the time
    • Catalytic ubiquitin-ligase residues not fully mapped
    • Relationship between HAT and E3 domains structurally unresolved
  10. 2013 Medium

    Extended the dual-enzyme model to Hedgehog signaling, showing KAT2B both supports GLI1-dependent H3K9ac on target promoters and ubiquitinates/acetylates GLI1 to terminate or restrain the pathway.

    Evidence Co-IP, ChIP, in vitro ubiquitination/acetylation, site mutagenesis, and in vivo tumor models

    PMID:23943798 PMID:24013724 PMID:25855960 PMID:26945969

    Open questions at the time
    • Context determining co-activator vs degradative role not resolved
    • Direct GLI1 acetylation not reconstituted in every report
  11. 2016 High

    Broadened the non-histone substrate repertoire to cell-cycle and centrosome control, identifying acetylation of cdk2 (K33), p27 (K100), and PLK4 (K45/K46) as inhibitory modifications.

    Evidence Acetylome proteomics, in vitro acetylation with site mutagenesis, kinase assays, and centrosome/cell-cycle phenotypes

    PMID:19773423 PMID:22547391 PMID:27796307

    Open questions at the time
    • Endogenous stoichiometry of these acetylations unclear
    • Erasers not identified for all sites
  12. 2014 High

    Established KAT2B as a metabolic regulator of hepatic gluconeogenesis through both histone and non-histone routes, with pharmacological tractability for glycemic control.

    Evidence In vitro acetylation, ChIP, liver-specific knockdown/overexpression, and small-molecule inhibitor in metabolic mouse models

    PMID:24051374 PMID:25497092

    Open questions at the time
    • Reconciliation of gluconeogenesis-promoting (H3K9ac/CRTC2) and -suppressing (PGC-1α degradation) roles not fully integrated
    • Tissue selectivity of inhibitor not detailed
  13. 2017 High

    Placed KAT2B in the DNA-damage response, showing DNA-PK-activated KAT2B acetylates RPA1 at K163 to promote XPA recruitment and nucleotide excision repair.

    Evidence In vitro acetylation with site mapping, upstream kinase and eraser identification, and pathway-specific NER assays

    PMID:28854354

    Open questions at the time
    • Selectivity for NER over other repair pathways mechanism not fully explained
    • Single lab
  14. 2020 High

    Defined a replication-fork function in which KAT2B acetylates H4K8 to recruit nucleases for fork degradation, with ATR phosphorylation at S264 acting as a brake relevant to PARPi resistance.

    Evidence Chromatin fractionation, in vitro H4K8 acetylation, phospho-site mapping, H4K8ac-binding domain analysis, and PARPi resistance assays

    PMID:32966758

    Open questions at the time
    • How fork-localized KAT2B is initially recruited not fully defined
    • Relevance across BRCA-proficient contexts not established
  15. 2014 Medium

    Uncovered a HAT-independent cytoplasmic function, with KAT2B and GCN5 inhibiting TBK1 to negatively regulate IFN-β, decoupling an immune role from histone acetylation.

    Evidence HAT-dead mutants, genetic knockout, cytoplasmic TBK1 inhibition, and IFN-β assays

    PMID:25269644

    Open questions at the time
    • Direct biochemical mode of TBK1 inhibition not resolved
    • Single lab

Open questions

Synthesis pass · forward-looking unresolved questions
  • How KAT2B's two catalytic activities (acetyltransferase and E3 ubiquitin ligase), its autoacetylation-controlled localization, and its many context-dependent substrates are coordinated and selected in a given cell remains unresolved.
  • No structural model integrating HAT and ubiquitin-ligase activities
  • Substrate-selection rules in vivo undefined
  • Functional separation from GCN5 across tissues incomplete

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 8 GO:0140096 catalytic activity, acting on a protein 7 GO:0140110 transcription regulator activity 4 GO:0016874 ligase activity 3 GO:0042393 histone binding 2
Localization
GO:0005634 nucleus 3 GO:0000228 nuclear chromosome 2 GO:0005829 cytosol 2
Pathway
R-HSA-74160 Gene expression (Transcription) 5 R-HSA-162582 Signal Transduction 4 R-HSA-1640170 Cell Cycle 4 R-HSA-1266738 Developmental Biology 3 R-HSA-1430728 Metabolism 2 R-HSA-168256 Immune System 2 R-HSA-73894 DNA Repair 2
Partners
EP300GCN5GLI1MDM2MYOD1SIRT1SMAD3TP53
Complex memberships
PRC2 (associated via EZH2)STAGA/TFTC/ATACcdk8-Mediator (T/G-Mediator)

Evidence

Reading pass · 55 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1999 PCAF acetylates p53 in vitro at lysine K320, a residue distinct from that acetylated by p300, and this acetylation increases p53's ability to bind its cognate DNA site. Acetylation at this site is detected in vivo and increases in response to DNA-damaging agents. In vitro acetyltransferase assay, site-specific acetylation antibodies, in vivo detection after DNA damage Molecular and cellular biology High 9891054
1997 PCAF directly interacts with MyoD and forms a multimeric complex with p300/CBP on promoter elements to drive myogenic differentiation; the histone acetyltransferase activity of PCAF (but not p300) is required for p21 expression and terminal cell-cycle arrest during myogenesis. Anti-PCAF antibody microinjection, exogenous expression, reporter assays, HAT-dead mutant analysis Molecular cell High 9659901
1998 PCAF directly associates with the DNA-binding domain of nuclear receptors (RXR/RAR heterodimer) in a ligand-dependent manner and independently of p300/CBP, defining a novel cofactor interaction surface on nuclear receptors. Ligand-dependent recruitment from mammalian cell extracts, in vitro direct binding assay, transcription reporter assays Genes & development Medium 9620851
1999 Adenoviral E1A directly binds PCAF independently of CBP and inhibits PCAF HAT activity in vitro, blocking nucleosomal histone modification by the PCAF complex and p53 acetylation. In vitro HAT assay, direct binding assay, in vivo transcription assays Cell High 10025405 9687513
1998 PCAF forms a ternary complex with p300 and HIV-1 Tat in cells, and the HAT activity of PCAF (but not p300) is specifically required for Tat transactivation of integrated (but not unintegrated) HIV-1 LTR. Co-immunoprecipitation (ternary complex), dominant-negative HAT mutant transfection, integrated vs. unintegrated LTR reporter assays The Journal of biological chemistry High 9733796
2000 Mouse Notch1 intracellular region (RAMIC) directly interacts with mouse PCAF (and GCN5) through its ankyrin repeats and transactivation domain, recruiting PCAF to RBP-J and facilitating RBP-J-mediated transactivation; the N-terminal regions of PCAF/GCN5 are required for this interaction. Co-immunoprecipitation, domain mapping, transactivation reporter assays, E1A and Twist inhibition The Journal of biological chemistry Medium 10747963
1998 Human GCN5 and P/CAF have extended homologous amino-terminal domains enabling acetylation of nucleosomal substrates (not only free histones), unlike the shorter yeast Gcn5p; both interact with CBP/p300 and display similar substrate specificities for histone H3. In vitro HAT assay with nucleosomal and free histone substrates, protein interaction assays, immunodetection Molecular and cellular biology High 9742083
2000 Kinetic mechanism of human P/CAF follows a fully ordered Bi-Bi mechanism: acetyl-CoA binds first to free enzyme (Kd = 0.64 µM), then histone H3 peptide binds; chemical catalysis (acetyl transfer to Lys-14 of H3) is rate-determining; Glu-570 is the proposed general base catalyst. Bi-substrate kinetic analysis, product inhibition, equilibrium dialysis, pre-steady-state quench-flow, pH-dependent activity measurements Biochemistry High 11009610
2003 P/CAF auto-acetylates itself intramolecularly at five lysines (416–442) within its nuclear localization signal (NLS) at the C-terminus, and intermolecularly via its N-terminal domain; P/CAF is also acetylated by p300 but not CBP; auto-acetylation increases PCAF HAT activity. In vitro acetylation assay, mutational analysis, in vivo acetylation detection Nucleic acids research Medium 12888487
2008 P/CAF autoacetylation at the NLS (C-terminal lysines) controls its nuclear localization; HAT-inactive P/CAF accumulates in the cytoplasm; HDAC3 (and to a lesser extent HDAC1/2/4) deacetylates P/CAF leading to cytoplasmic accumulation; P/CAF accumulates in cytoplasm during apoptosis. Subcellular fractionation, fluorescence microscopy, HAT-dead mutant, HDAC overexpression/knockdown The Journal of biological chemistry Medium 19015268
2006 PCAF interacts physically with PTEN and acetylates PTEN at Lys125 and Lys128 within its catalytic cleft in a growth-factor-dependent manner, reducing PTEN's lipid phosphatase activity and thereby maintaining PI3K/AKT signaling; acetylation-resistant K125R/K128R mutants retain PI3K-suppressing and G1 arrest activities even with PCAF overexpression. Co-immunoprecipitation, in vitro acetyltransferase assay, shRNA knockdown, acetylation-resistant mutant analysis, cell cycle assays The Journal of biological chemistry High 16829519
2007 PCAF possesses an intrinsic ubiquitin E3 ligase activity (distinct from its acetyltransferase activity) that ubiquitinates Hdm2, promoting its degradation and thereby stabilizing p53; PCAF knockdown stabilizes Hdm2. PCAF knockdown in HeLa/U2OS cells, in vitro ubiquitination assay, domain deletion analysis Nature cell biology High 17293853
2002 PCAF acetylates HIV-1 Tat at Lys50; acetylated Tat binds the PCAF bromodomain; structural analysis defined critical interaction residues (Y47/R53 in Tat; V763/Y802/Y809 in PCAF bromodomain); mutations at these residues cumulatively inhibit Tat–PCAF interaction and synergistic HIV promoter activation. In vitro binding assay, in vivo co-immunoprecipitation, structural analysis of acetyl-peptide–bromodomain complex, site-directed mutagenesis, HIV promoter reporter assay The EMBO journal High 12032084
2000 P/CAF directly interacts with Smad3 (via Smad3 MH2 domain and P/CAF N-terminal region) upon TGF-β receptor activation; P/CAF potentiates TGF-β/Smad3-mediated transcription cooperatively with p300 and Smad4. In vitro GST pull-down, co-immunoprecipitation in mammalian cells, transcription reporter assays Nucleic acids research Medium 11058129
2000 P/CAF-mediated acetylation of TAL1/SCL maps to a lysine-rich motif in the loop region and increases TAL1 DNA binding while selectively inhibiting its interaction with co-repressor mSin3A; an acetylation-defective P/CAF mutant inhibits TAL1 acetylation, DNA binding, transcription, and terminal erythroid differentiation. In vitro acetylation assay, domain mapping, DNA-binding EMSA, co-immunoprecipitation, differentiation assay with HAT-dead mutant The EMBO journal High 11118214
2000 Mice lacking PCAF are developmentally normal; in PCAF-null mice, PCAF-B/GCN5 protein levels are drastically elevated in tissues where PCAF is normally abundant, indicating functional compensation between PCAF and GCN5. Gene knockout mouse model, Western blot quantification of compensatory protein levels Proceedings of the National Academy of Sciences of the United States of America High 11027331
2001 Cyclin D1 and the androgen receptor (AR) both bind overlapping domains of P/CAF; cyclin D1 displaces AR binding to P/CAF in vitro; the HAT activity of P/CAF rescues cyclin D1-mediated AR trans-repression. In vitro binding/displacement assay, HAT-dead P/CAF rescue experiment, reporter assays Molecular endocrinology (Baltimore, Md.) Medium 11328859
1999 Cyclin D1 interacts with the histone acetyltransferase P/CAF, facilitating P/CAF–estrogen receptor (ER) association; P/CAF potentiates cyclin D1-stimulated ER transcriptional activity in a dose-dependent, HAT-activity-dependent manner. Co-immunoprecipitation, reporter assay, HAT-dead mutant analysis Proceedings of the National Academy of Sciences of the United States of America Medium 10318892
2003 P/CAF acetylates the ETS transcription factor ER81 at K116 (and p300 additionally at K33); acetylation enhances ER81 transactivation, DNA binding activity, and in vivo half-life; oncogenic HER2/Neu stimulates p300-mediated ER81 acetylation via the Ras→Raf→MAPK pathway. In vitro and in vivo acetylation assay, acetylation-deficient mutant analysis, DNA binding assay, protein stability measurement Molecular and cellular biology Medium 12917345
1998 NF-Y complex associates with human GCN5 and P/CAF in vivo and possesses HAT activity through these interactions; the NF-YB:YC histone-fold motif is sufficient for stable interaction with GCN5 in vitro; deletion of N- or C-terminal regions of GCN5 disrupts NF-Y interaction. In vivo co-immunoprecipitation, in vitro interaction assay, deletion mutant analysis, transient transfection reporter assay The Journal of biological chemistry Medium 9430679
2002 The adenovirus E1B 55-kDa oncoprotein specifically inhibits PCAF-mediated acetylation of p53 at K320 in vivo and in vitro (without affecting histone acetylation or PCAF autoacetylation) by physically binding PCAF and interfering with the PCAF–p53 interaction. In vitro acetylation assay, co-immunoprecipitation, competition assay, in vivo acetylation measurement Molecular and cellular biology Medium 10891493
2002 MDM2 interacts with PCAF in vitro and in cells and inhibits PCAF-mediated acetylation of p53 at K320 in vitro and in overexpression experiments, repressing PCAF-dependent p53 transcriptional activation without affecting p53 levels. GST pull-down, co-immunoprecipitation, in vitro acetylation assay, reporter assay, chromatin immunoprecipitation The Journal of biological chemistry Medium 12068014
2008 PCAF associates with actin and hnRNP U in nuclear extracts via affinity chromatography and protein-protein interaction assays; PCAF-associated HAT activity is released by disruption of the actin–hnRNP U complex; PCAF, actin, and hnRNP U co-occupy promoters and coding regions of pol II genes and associate with nascent ribonucleoprotein complexes, supporting a role for PCAF in pol II transcription elongation. Affinity chromatography, co-immunoprecipitation, biochemical fractionation, chromatin immunoprecipitation, RNA immunoprecipitation, bromouridine incorporation assay Molecular and cellular biology Medium 18710935
2008 PCAF and HDAC4 associate with cardiac sarcomeres (Z-disc and I/A bands) in cardiomyocytes; PCAF acetylates the Z-disc protein MLP (muscle LIM protein); increased acetylation via HDAC inhibition enhances myofilament calcium sensitivity in wild-type but not MLP-knockout mice. Immunohistochemistry, electron microscopy, co-immunoprecipitation, in vitro acetylation assay, calcium sensitivity measurement, knockout comparison The Journal of biological chemistry Medium 18250163
2008 PCAF acetylates β-catenin, inhibiting its ubiquitination and increasing its stability; the key ubiquitination sites K19 and K49 of β-catenin are the critical residues for PCAF-induced acetylation and stabilization; PCAF knockdown reduces β-catenin protein level, transcriptional activity, promotes differentiation, and inhibits migration. Co-immunoprecipitation, in vitro acetylation assay, site-directed mutagenesis, ubiquitination assay, shRNA knockdown, cell migration and differentiation assays Molecular biology of the cell High 18987336
2013 PCAF/KAT2B interacts with GLI1 (downstream effector of Hedgehog signaling) and is required for H3K9 acetylation on Hh target gene promoters; PCAF depletion impairs Hh target gene expression, reduces medulloblastoma/glioblastoma cell proliferation, and reduces tumor-forming potential of neural stem cells in vivo. Co-immunoprecipitation, siRNA knockdown, ChIP for H3K9ac, proliferation assay, apoptosis assay, in vivo neural stem cell tumor assay Cancer research Medium 23943798
2013 PCAF acts as a novel E3 ubiquitin ligase for GLI1; in response to genotoxic stress, p53-elevated PCAF promotes GLI1 ubiquitination and proteasome-dependent degradation (requires the ubiquitination domain of PCAF, not a deletion mutant lacking it), thereby inhibiting Hedgehog signaling. In vitro ubiquitination assay, deletion mutant of PCAF ubiquitination domain, shRNA knockdown of p53/PCAF, GLI1 rescue experiment, in vivo medulloblastoma model Cell death and differentiation High 24013724
2013 KAT2B (PCAF) is recruited by dephosphorylated CRTC2 to gluconeogenic gene promoters, where it acetylates H3K9 (H3K9Ac); KAT2B cooperates with WDR5 to stimulate the gluconeogenic program; depletion of KAT2B or WDR5 decreases gluconeogenic gene expression and a KAT2B antagonist lowers blood glucose in insulin-resistant mice. Mouse knockout/knockdown models, in vitro acetylation assays, ChIP, small-molecule KAT2B inhibitor in vivo The Journal of clinical investigation High 24051374
2016 KAT2A and KAT2B (PCAF) acetylate PLK4 kinase domain at K45 and K46; K45/K46 acetylation impairs PLK4 kinase activity in vitro (molecular dynamics suggests shift to inactive conformation); the PLK4 K45R/K46R mutant does not cause centrosome overamplification when overexpressed; impairing KAT2A/2B acetyltransferase activity results in excess centrosome numbers in cells. Shotgun proteomics acetylome, in vitro kinase assay after acetylation, molecular dynamics modelling, mutant overexpression centrosome assay Nature communications High 27796307
2014 PCAF acetylates PGC-1α at K328 and K450, triggering its proteasomal degradation and suppressing gluconeogenic transcriptional activity; adenoviral PCAF expression in obese mouse liver represses gluconeogenic enzyme activation and improves glucose homeostasis; liver-specific PCAF knockdown increases blood glucose. In vitro acetylation assay, site-directed mutagenesis, adenoviral overexpression in vivo, liver-specific knockdown, glucose tolerance tests Cell reports High 25497092
2015 PCAF acetylates EZH2 primarily at K348; K348 acetylation decreases EZH2 phosphorylation at T345 and T487, increases EZH2 stability without disrupting PRC2 formation, and enhances EZH2's capacity to suppress target genes; SIRT1 deacetylates EZH2. Co-immunoprecipitation, in vitro acetylation assay, site-directed mutagenesis, protein stability assay, PRC2 complex immunoprecipitation Nucleic acids research Medium 25800736
2003 PCAF directly interacts with cdk2; PCAF inhibits cyclin/cdk2 activity by two mechanisms: (i) disrupting cyclin–cdk2 interaction and (ii) acetylating cdk2 at K33 (within the ATP-binding pocket), thereby inhibiting cdk2 kinase activity; PCAF overexpression arrests cells at S and G2/M in a cdk2-dependent manner. Co-immunoprecipitation, in vitro kinase assay, in vitro acetylation with K33 mutagenesis, cell cycle analysis, cdk2 rescue overexpression Nucleic acids research Medium 19773423
2010 During keratinocyte differentiation, PCAF acetylates Rb at sites within its nuclear localization sequence; PCAF HAT activity is required for normal differentiation; acetylation-deficient Rb is mislocalized to the cytoplasm during differentiation, and SIRT1 overexpression or PCAF reduction causes the same Rb mislocalization. shRNA depletion, HAT-dead PCAF, acetylation-site Rb mutants, subcellular fractionation/immunofluorescence, differentiation assays Journal of cell science Medium 20940255
2008 PCAF is an HIF-1α cofactor that acetylates HIF-1α under hypoxia-mimicking conditions; PCAF-mediated acetylation of p53 at K320 is preferentially reduced under hypoxia compared to K382 acetylation (by p300), redirecting acetylated p53 to p21 promoters while preventing recruitment to pro-apoptotic BID promoter. Co-immunoprecipitation, chromatin immunoprecipitation, acetylation site-specific analysis under hypoxia Oncogene Medium 18574470
2012 PCAF HAT activity is required for stress-induced H3K9 and H3K14 acetylation at the p21 promoter and for p53-dependent p21 transcription in response to multiple stresses (nutlin-3, DNA damage, p14ARF); this role is independent of p53 K320 acetylation; PCAF loss prevents cell cycle arrest. siRNA knockdown, HAT-dead PCAF mutant, chromatin immunoprecipitation for H3K9ac/H3K14ac, p53 promoter occupancy assay, cell cycle analysis Cell cycle (Georgetown, Tex.) Medium 22713239
2017 PCAF/GCN5 PCAF-deficient macrophages exhibit markedly reduced cytokine production upon LPS stimulation; chemical inhibition of PCAF/GCN5 bromodomains alone is insufficient to recapitulate this immune phenotype, but PCAF/GCN5 PROTAC-mediated degradation potently modulates inflammatory mediator expression. PCAF-deficient macrophage functional assay, bromodomain inhibitor comparison, PROTAC-mediated degradation, cytokine expression assay ACS chemical biology Medium 30200762
2017 PCAF acetylates RPA1 at K163; DNA-PK phosphorylates and activates PCAF upon UV damage to promote K163 acetylation; K163 acetylation of RPA1 is critical for accumulation of XPA at damaged DNA and activation of nucleotide excision repair (NER) specifically; HDAC6 and SIRT1 deacetylate RPA1. In vitro acetylation assay, co-immunoprecipitation, site-directed mutagenesis, NER assay, XPA recruitment to damage sites, HDAC identification Cell reports High 28854354
2020 PCAF is a fork-associated protein at stalled replication forks; PCAF acetylates H4K8 at stalled forks, recruiting MRE11 and EXO1 (via an H4K8ac-binding domain) to promote fork degradation in BRCA-deficient cells; ATR phosphorylates PCAF at S264 to limit its fork association and activity; low PCAF levels stabilize stalled forks and cause PARPi resistance in BRCA-deficient cells. Chromatin fractionation at forks, in vitro H4K8 acetylation assay, S264 phospho-site mapping, MRE11/EXO1 H4K8ac binding domain analysis, PARPi resistance assay, ATR inhibition Molecular cell High 32966758
2009 hSIRT1 deacetylates PCAF in vitro and modulates PCAF acetylation in vivo; hSIRT1 represses E2F1-dependent p73 promoter activity by forming a hSIRT1–PCAF–E2F1 complex on the P1p73 promoter; upon apoptotic DNA damage, decreased nuclear NAD+ inactivates hSIRT1's deacetylase activity (without disrupting SIRT1–PCAF interaction), releasing PCAF to form active PCAF/E2F1 complexes on the P1p73 promoter. In vitro deacetylation assay, co-immunoprecipitation, chromatin immunoprecipitation, NAD+ manipulation, reporter assay Molecular and cellular biology Medium 19188449
2010 PTH treatment increases PCAF acetylation at the MMP-13 promoter in a p300-dependent manner; p300-dependent PCAF acetylation and mutual p300–PCAF promoter recruitment is required for PTH-induced MMP-13 transcription; HAT activities of both PCAF and p300 are additively required. ChIP, siRNA knockdown of PCAF/p300/Runx2, reporter assay with HAT-dead mutants The Journal of biological chemistry Medium 20870727
2013 PCAF directly acetylates cytoplasmic GLI1 at K518, preventing its nuclear translocation and promoter occupancy, suppressing Hedgehog signaling in hepatocellular carcinoma; PCAF-mediated GLI1 acetylation reduces BCL2 expression and upregulates BAX. In vitro acetylation assay, site-directed mutagenesis, subcellular fractionation, ChIP, in vivo xenograft model Cell death & disease Medium 25855960
2008 GCN5L (KAT2B) stably associates with Mediator containing the cdk8 subcomplex (T/G-Mediator) together with TRRAP; cdk8 phosphorylates H3 serine-10, which then stimulates H3K14 acetylation by GCN5L within the complex, producing tandem H3 phosphoacetylation that correlates with transcriptional activation. Reconstituted human transcription system, co-immunoprecipitation, in vitro histone modification assay, cdk8 knockdown, ChIP The EMBO journal High 18418385
2014 PCAF (Kat2b) and Gcn5 negatively regulate IFN-β production through a HAT-independent, non-transcriptional mechanism by inhibiting the innate immune kinase TBK1 in the cytoplasm; Gcn5/PCAF-mediated H3K9ac is dispensable for IFNB expression and the vast majority of active genes in fibroblasts. HAT-dead mutant analysis, cytoplasmic TBK1 inhibition assay, genetic knockout of Gcn5/PCAF, IFN-β production assay EMBO reports Medium 25269644
2013 PCAF acetylates HOXA10 at K338 and K339, inhibiting HOXA10-mediated ITGB3 (β3-integrin) transcription and thereby impairing endometrial receptivity and embryo adhesion. Co-immunoprecipitation, in vitro acetylation assay with site mapping, luciferase reporter assay, ChIP, BeWo spheroid attachment assay, PCAF overexpression/knockdown The Journal of clinical endocrinology and metabolism Medium 24037888
2012 PCAF directly interacts with p27Kip1 (via aa 91–120 of p27; PCAF catalytic domain required) and acetylates p27 at K100; PCAF overexpression induces proteasomal degradation of p27 (Skp2-independent); K100R mutant p27 is resistant to PCAF-induced degradation and remains stable through the cell cycle. Co-immunoprecipitation, in vitro acetylation assay, K100R mutagenesis, protein half-life measurement, proteasome inhibitor assay, ubiquitylation assay Nucleic acids research Medium 22547391
2020 PCAF-dependent acetylation of ISX at K69 promotes ISX interaction with BRD4 (acetylated at K332) and nuclear translocation of the ISX–BRD4 complex; in the nucleus, the complex binds EMT gene promoters with H3K9/K14/K18 acetylation, activating EMT transcription and cancer metastasis. Co-immunoprecipitation, site-directed mutagenesis, subcellular fractionation, ChIP, histone acetylation analysis EMBO reports Medium 31908141
2020 SIRT7 directly interacts with PCAF and deacetylates PCAF at K720 upon glucose deprivation; deacetylated PCAF binds MDM2 more strongly, promoting MDM2 ubiquitination/degradation, elevating p53, and inducing p21/cell-cycle arrest. Co-immunoprecipitation, in vitro deacetylation assay, K720 site mapping, MDM2 ubiquitination assay, cell cycle analysis Oncogene Medium 32404984
2013 PCAF interacts with GLI1 and ubiquitinates it (E3 ubiquitin ligase activity), inhibiting HCC cell metastasis and EMT; PCAF-mediated GLI1 targeting suppresses Gli1-driven EMT in hepatocellular carcinoma. Co-immunoprecipitation, in vitro ubiquitination assay, siRNA knockdown, migration/invasion assay Cancer letters Medium 26945969
2017 PCAF acetylates MKL1 in response to pro-inflammatory stimuli (TNF-α, LPS); acetylation promotes MKL1 nuclear enrichment, enhances MKL1–NF-κB interaction, and stabilizes MKL1 binding to NF-κB target promoters, activating pro-inflammatory transcription. Co-immunoprecipitation, in vivo acetylation analysis, MKL1 lysine mutant functional assay, nuclear fractionation, ChIP Biochimica et biophysica acta. Gene regulatory mechanisms Medium 28571745
2013 PCAF regulates the EB1–TIP150 interaction at kinetochore microtubule plus ends by acetylating EB1; persistent EB1 acetylation by PCAF perturbs the EB1–TIP150 interaction, leading to metaphase alignment defects, spindle checkpoint activation, and chromosome aneuploidy. siRNA knockdown, co-immunoprecipitation, acetylation assay, live cell imaging of chromosome alignment, spindle checkpoint assay The Journal of biological chemistry Medium 23595990
2018 PCAF (and GCN5) acetylate influenza A virus nucleoprotein (NP) in vitro; PCAF acetylates NP at Lys-31 while GCN5 targets Lys-90; PCAF silencing increases viral polymerase activity, indicating that PCAF-mediated NP acetylation at K31 negatively regulates influenza replication. In vitro acetyltransferase assay, LC-MS site identification, RNAi silencing, viral polymerase activity assay The Journal of biological chemistry Medium 29555684
2003 PCAF binds p73; the N-terminal transactivation domain and OD of p73 and the HAT domain of PCAF are required for interaction; PCAF HAT activity is required for stimulating p73-mediated transactivation of p21 and induction of apoptosis. Co-immunoprecipitation, domain mapping, reporter assay, PCAF-specific siRNA, colony formation assay Oncogene Medium 14614455
2010 PCAF and CBP/p300 are repressed by inhibitory (myelin, CSPG) substrates in neurons; HDAC inhibition restores PCAF/CBP/p300 expression; PCAF and CBP/p300 acetylate histone H3 at K9–14 and p53, initiating a pro-neuronal outgrowth transcriptional program that promotes axon growth and counteracts growth cone collapse. Gene silencing, gain-of-function, chromatin acetylation analysis, neurite outgrowth quantification Cell death and differentiation Medium 20094059
2014 Gcn5 and PCAF double knockout blocks PPARγ expression (preventing adipocyte differentiation) and is essential for Prdm16 expression (required for brown adipogenesis); Gcn5/PCAF facilitate PPARγ RNA pol II recruitment and Prdm16 transcript elongation. Double-knockout cell lines, PPARγ rescue expression, ChIP for pol II, nascent RNA analysis Molecular and cellular biology Medium 25071153
2010 HIPK2 cooperates with PCAF to induce PCAF-mediated p53 acetylation after nonapoptotic DNA damage; HIPK2 depletion abolishes PCAF-dependent p53 acetylation and reduces p53 binding to the p21Waf1 promoter, inhibiting p21 transactivation and allowing cell proliferation. RNAi knockdown of HIPK2, ChIP, acetylation analysis, cell cycle assay Oncogene Medium 15897882

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2010 Distinct roles of GCN5/PCAF-mediated H3K9ac and CBP/p300-mediated H3K18/27ac in nuclear receptor transactivation. The EMBO journal 673 21131905
1999 p53 sites acetylated in vitro by PCAF and p300 are acetylated in vivo in response to DNA damage. Molecular and cellular biology 658 9891054
1997 Differential roles of p300 and PCAF acetyltransferases in muscle differentiation. Molecular cell 372 9659901
1998 The histone acetylase PCAF is a nuclear receptor coactivator. Genes & development 327 9620851
2007 Distinct GCN5/PCAF-containing complexes function as co-activators and are involved in transcription factor and global histone acetylation. Oncogene 317 17694077
1999 A viral mechanism for inhibition of p300 and PCAF acetyltransferase activity. Cell 297 10025405
1998 Activation of integrated provirus requires histone acetyltransferase. p300 and P/CAF are coactivators for HIV-1 Tat. The Journal of biological chemistry 268 9733796
2002 p300 and PCAF act cooperatively to mediate transcriptional activation from chromatin templates by notch intracellular domains in vitro. Molecular and cellular biology 217 12391150
2006 PCAF modulates PTEN activity. The Journal of biological chemistry 190 16829519
2000 Functional interaction between the mouse notch1 intracellular region and histone acetyltransferases PCAF and GCN5. The Journal of biological chemistry 187 10747963
2000 Distinct but overlapping roles of histone acetylase PCAF and of the closely related PCAF-B/GCN5 in mouse embryogenesis. Proceedings of the National Academy of Sciences of the United States of America 187 11027331
2010 HDAC inhibition promotes neuronal outgrowth and counteracts growth cone collapse through CBP/p300 and P/CAF-dependent p53 acetylation. Cell death and differentiation 162 20094059
2005 Isothiazolones as inhibitors of PCAF and p300 histone acetyltransferase activity. Molecular cancer therapeutics 160 16227401
2001 Cyclin D1 binds the androgen receptor and regulates hormone-dependent signaling in a p300/CBP-associated factor (P/CAF)-dependent manner. Molecular endocrinology (Baltimore, Md.) 153 11328859
2007 Intrinsic ubiquitination activity of PCAF controls the stability of the oncoprotein Hdm2. Nature cell biology 149 17293853
1999 P/CAF associates with cyclin D1 and potentiates its activation of the estrogen receptor. Proceedings of the National Academy of Sciences of the United States of America 148 10318892
2018 Modulating PCAF/GCN5 Immune Cell Function through a PROTAC Approach. ACS chemical biology 134 30200762
2002 Down regulation of the interleukin-8 promoter by human papillomavirus type 16 E6 and E7 through effects on CREB binding protein/p300 and P/CAF. Journal of virology 134 12163591
2008 HDAC4 and PCAF bind to cardiac sarcomeres and play a role in regulating myofilament contractile activity. The Journal of biological chemistry 132 18250163
1998 NF-Y is associated with the histone acetyltransferases GCN5 and P/CAF. The Journal of biological chemistry 132 9430679
1998 Mammalian GCN5 and P/CAF acetyltransferases have homologous amino-terminal domains important for recognition of nucleosomal substrates. Molecular and cellular biology 129 9742083
2008 The histone acetyltransferase PCAF associates with actin and hnRNP U for RNA polymerase II transcription. Molecular and cellular biology 119 18710935
2003 Role of p300 and PCAF in regulating cyclooxygenase-2 promoter activation by inflammatory mediators. Blood 118 14630807
2002 Transcriptional synergy between Tat and PCAF is dependent on the binding of acetylated Tat to the PCAF bromodomain. The EMBO journal 115 12032084
2001 Transcription factor-dependent regulation of CBP and P/CAF histone acetyltransferase activity. The EMBO journal 115 11296231
2015 PCAF-primed EZH2 acetylation regulates its stability and promotes lung adenocarcinoma progression. Nucleic acids research 114 25800736
1998 E1A directly binds and regulates the P/CAF acetyltransferase. The EMBO journal 110 9687513
2008 PCAF acetylates {beta}-catenin and improves its stability. Molecular biology of the cell 104 18987336
2016 KAT2A/KAT2B-targeted acetylome reveals a role for PLK4 acetylation in preventing centrosome amplification. Nature communications 99 27796307
2013 Histone acetyltransferase PCAF is required for Hedgehog-Gli-dependent transcription and cancer cell proliferation. Cancer research 96 23943798
2003 Interaction of the HPV E7 proteins with the pCAF acetyltransferase. Oncogene 94 12813456
2000 The transcriptional co-activator P/CAF potentiates TGF-beta/Smad signaling. Nucleic acids research 94 11058129
2003 Acetylation-mediated transcriptional activation of the ETS protein ER81 by p300, P/CAF, and HER2/Neu. Molecular and cellular biology 92 12917345
2003 Mechanisms of P/CAF auto-acetylation. Nucleic acids research 91 12888487
2008 PCAF is an HIF-1alpha cofactor that regulates p53 transcriptional activity in hypoxia. Oncogene 90 18574470
2008 Cooperative activity of cdk8 and GCN5L within Mediator directs tandem phosphoacetylation of histone H3. The EMBO journal 88 18418385
2001 Interaction of EVI1 with cAMP-responsive element-binding protein-binding protein (CBP) and p300/CBP-associated factor (P/CAF) results in reversible acetylation of EVI1 and in co-localization in nuclear speckles. The Journal of biological chemistry 80 11568182
2015 Histone acetyltransferase PCAF regulates inflammatory molecules in the development of renal injury. Epigenetics 79 25496441
2000 Adenovirus E1B 55-kilodalton oncoprotein inhibits p53 acetylation by PCAF. Molecular and cellular biology 79 10891493
2013 PCAF ubiquitin ligase activity inhibits Hedgehog/Gli1 signaling in p53-dependent response to genotoxic stress. Cell death and differentiation 77 24013724
2012 The histone acetyltransferase PCAF regulates p21 transcription through stress-induced acetylation of histone H3. Cell cycle (Georgetown, Tex.) 73 22713239
2000 P/CAF-mediated acetylation regulates the function of the basic helix-loop-helix transcription factor TAL1/SCL. The EMBO journal 73 11118214
2002 MDM2 inhibits PCAF (p300/CREB-binding protein-associated factor)-mediated p53 acetylation. The Journal of biological chemistry 72 12068014
2000 Kinetic mechanism of human histone acetyltransferase P/CAF. Biochemistry 72 11009610
2017 PCAF/GCN5-Mediated Acetylation of RPA1 Promotes Nucleotide Excision Repair. Cell reports 67 28854354
2013 Glucagon regulates gluconeogenesis through KAT2B- and WDR5-mediated epigenetic effects. The Journal of clinical investigation 67 24051374
2001 Functional interaction between coactivators CBP/p300, PCAF, and transcription factor FKLF2. The Journal of biological chemistry 64 11748222
2016 Discovery of a PCAF Bromodomain Chemical Probe. Angewandte Chemie (International ed. in English) 63 27966810
2005 HIPK2 contributes to PCAF-mediated p53 acetylation and selective transactivation of p21Waf1 after nonapoptotic DNA damage. Oncogene 61 15897882
2020 PCAF-Mediated Histone Acetylation Promotes Replication Fork Degradation by MRE11 and EXO1 in BRCA-Deficient Cells. Molecular cell 60 32966758
2009 hSirT1-dependent regulation of the PCAF-E2F1-p73 apoptotic pathway in response to DNA damage. Molecular and cellular biology 59 19188449
2014 Gcn5 and PCAF regulate PPARγ and Prdm16 expression to facilitate brown adipogenesis. Molecular and cellular biology 57 25071153
2008 Autoacetylation regulates P/CAF nuclear localization. The Journal of biological chemistry 57 19015268
2013 Lysine acetyltransferase PCAF is a key regulator of arteriogenesis. Arteriosclerosis, thrombosis, and vascular biology 56 23788761
2013 PCAF impairs endometrial receptivity and embryo implantation by down-regulating β3-integrin expression via HOXA10 acetylation. The Journal of clinical endocrinology and metabolism 56 24037888
2003 M phase-specific phosphorylation of BRCA2 by Polo-like kinase 1 correlates with the dissociation of the BRCA2-P/CAF complex. The Journal of biological chemistry 55 12815053
2014 PCAF improves glucose homeostasis by suppressing the gluconeogenic activity of PGC-1α. Cell reports 54 25497092
2019 MicroRNA-29a represses osteoclast formation and protects against osteoporosis by regulating PCAF-mediated RANKL and CXCL12. Cell death & disease 53 31543513
2012 A novel function for p21Cip1 and acetyltransferase p/CAF as critical transcriptional regulators of TGFβ-mediated breast cancer cell migration and invasion. Breast cancer research : BCR 52 22995475
2010 Acetylation of Rb by PCAF is required for nuclear localization and keratinocyte differentiation. Journal of cell science 52 20940255
2012 Cooperation of p300 and PCAF in the control of microRNA 200c/141 transcription and epithelial characteristics. PloS one 51 22384255
2006 Regulation of histone acetyltransferase activity of p300 and PCAF by proto-oncogene protein DEK. FEBS letters 51 16696975
2015 Histone acetyltransferase PCAF accelerates apoptosis by repressing a GLI1/BCL2/BAX axis in hepatocellular carcinoma. Cell death & disease 47 25855960
2013 Histone acetyltransferase PCAF up-regulated cell apoptosis in hepatocellular carcinoma via acetylating histone H4 and inactivating AKT signaling. Molecular cancer 46 23981651
2020 PCAF-mediated acetylation of ISX recruits BRD4 to promote epithelial-mesenchymal transition. EMBO reports 44 31908141
2018 Influenza A virus nucleoprotein is acetylated by histone acetyltransferases PCAF and GCN5. The Journal of biological chemistry 42 29555684
2018 Nonhistone targets of KAT2A and KAT2B implicated in cancer biology 1. Biochemistry and cell biology = Biochimie et biologie cellulaire 41 29671337
2020 Complex functions of Gcn5 and Pcaf in development and disease. Biochimica et biophysica acta. Gene regulatory mechanisms 40 32730897
2018 Kat2a and Kat2b Acetyltransferase Activity Regulates Craniofacial Cartilage and Bone Differentiation in Zebrafish and Mice. Journal of developmental biology 40 30424580
2014 Gcn5 and PCAF negatively regulate interferon-β production through HAT-independent inhibition of TBK1. EMBO reports 40 25269644
2004 HIV-1 Tat interactions with p300 and PCAF transcriptional coactivators inhibit histone acetylation and neurotrophin signaling through CREB. The Journal of biological chemistry 40 15611041
2020 Design, synthesis, and antitumor activity of novel compounds based on 1,2,4-triazolophthalazine scaffold: Apoptosis-inductive and PCAF-inhibitory effects. Bioorganic chemistry 39 32615465
2017 The epigenetic factor PCAF regulates vascular inflammation and is essential for intimal hyperplasia development. PloS one 39 29016683
2008 Inhibition of the PCAF histone acetyl transferase and cell proliferation by isothiazolones. Bioorganic & medicinal chemistry 39 19111471
2003 p300/CBP-associated factor (P/CAF) interacts with nuclear respiratory factor-1 to regulate the UDP-N-acetyl-alpha-d-galactosamine: polypeptide N-acetylgalactosaminyltransferase-3 gene. The Biochemical journal 39 12720548
2016 PCAF inhibits hepatocellular carcinoma metastasis by inhibition of epithelial-mesenchymal transition by targeting Gli-1. Cancer letters 38 26945969
2016 P300/CBP-associated factor (PCAF) inhibits the growth of hepatocellular carcinoma by promoting cell autophagy. Cell death & disease 38 27711074
2010 Enhanced expression of PCAF endows apoptosis resistance in cisplatin-resistant cells. Molecular cancer research : MCR 38 20530585
2010 Parathyroid hormone activation of matrix metalloproteinase-13 transcription requires the histone acetyltransferase activity of p300 and PCAF and p300-dependent acetylation of PCAF. The Journal of biological chemistry 38 20870727
2020 The regulation of acetylation and stability of HMGA2 via the HBXIP-activated Akt-PCAF pathway in promotion of esophageal squamous cell carcinoma growth. Nucleic acids research 37 32313942
2003 Functional interplay between CBP and PCAF in acetylation and regulation of transcription factor KLF13 activity. Journal of molecular biology 37 12758070
2001 Functional interaction between p/CAF and human papillomavirus E2 protein. The Journal of biological chemistry 36 11744716
2020 SIRT7 activates p53 by enhancing PCAF-mediated MDM2 degradation to arrest the cell cycle. Oncogene 35 32404984
2010 Restoration of DNA-binding and growth-suppressive activity of mutant forms of p53 via a PCAF-mediated acetylation pathway. Journal of cellular physiology 35 20589832
2009 The transcriptional co-activator PCAF regulates cdk2 activity. Nucleic acids research 35 19773423
2007 Concerted activation of the Mdm2 promoter by p72 RNA helicase and the coactivators p300 and P/CAF. Journal of cellular biochemistry 35 17226766
2017 Acetylation of MKL1 by PCAF regulates pro-inflammatory transcription. Biochimica et biophysica acta. Gene regulatory mechanisms 34 28571745
2013 Regulation of a dynamic interaction between two microtubule-binding proteins, EB1 and TIP150, by the mitotic p300/CBP-associated factor (PCAF) orchestrates kinetochore microtubule plasticity and chromosome stability during mitosis. The Journal of biological chemistry 34 23595990
2019 Epigenetically Down-Regulated Acetyltransferase PCAF Increases the Resistance of Colorectal Cancer to 5-Fluorouracil. Neoplasia (New York, N.Y.) 32 31042625
2020 Non-histone protein acetylation by the evolutionarily conserved GCN5 and PCAF acetyltransferases. Biochimica et biophysica acta. Gene regulatory mechanisms 31 32711095
2022 hMSCs-derived exosome circCDK13 inhibits liver fibrosis by regulating the expression of MFGE8 through miR-17-5p/KAT2B. Cell biology and toxicology 30 35484432
2012 PCAF regulates the stability of the transcriptional regulator and cyclin-dependent kinase inhibitor p27 Kip1. Nucleic acids research 28 22547391
2021 P300/CBP-associated factor (PCAF) attenuated M1 macrophage inflammatory responses possibly through KLF2 and KLF4. Immunology and cell biology 27 33768642
2015 Selective PCAF inhibitor ameliorates cognitive and behavioral deficits by suppressing NF-κB-mediated neuroinflammation induced by Aβ in a model of Alzheimer's disease. International journal of molecular medicine 27 25672970
2012 A transcriptionally active pRb-E2F1-P/CAF signaling pathway is central to TGFβ-mediated apoptosis. Cell death & disease 27 23059826
2003 PCAF is a coactivator for p73-mediated transactivation. Oncogene 26 14614455
2014 Differential coupling of KLF10 to Sin3-HDAC and PCAF regulates the inducibility of the FOXP3 gene. American journal of physiology. Regulatory, integrative and comparative physiology 25 24944246
2010 PCAF interacts with XBP-1S and mediates XBP-1S-dependent transcription. Nucleic acids research 25 20817929
2021 In-vitro acetylation of SARS-CoV and SARS-CoV-2 nucleocapsid proteins by human PCAF and GCN5. Biochemical and biophysical research communications 22 33894414
2017 Differential Effects of Histone Acetyltransferase GCN5 or PCAF Knockdown on Urothelial Carcinoma Cells. International journal of molecular sciences 22 28678170

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