Affinage

IFIT2

Interferon-induced protein with tetratricopeptide repeats 2 · UniProt P09913

Length
472 aa
Mass
54.6 kDa
Annotated
2026-06-10
58 papers in source corpus 21 papers cited in narrative 23 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/7 claims corpus-supported (86%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

IFIT2 is an interferon-stimulated tetratricopeptide-repeat (TPR) protein that acts as an RNA-binding antiviral effector and immune signaling regulator (PMID:8660659, PMID:38888342). It forms a domain-swapped heterodimer with IFIT3 that recognizes viral mRNAs bearing short 5' UTRs (<50 nucleotides) as a non-self molecular pattern, binding such RNAs and inhibiting their translation to confer antiviral activity against viruses including VSV and parainfluenza virus 3; the 5' UTR length requirement is necessary and sufficient and operates independently of cap structure (PMID:41093992, PMID:40079162). Within the broader IFIT system, IFIT3 stabilizes IFIT1 and promotes its cap0 mRNA binding, and IFIT2–IFIT3 heterodimers form preferentially over homodimers and can further associate with IFIT1 (PMID:29554348). Beyond restricting viral mRNAs, IFIT2 binds AU-rich regions of cellular and viral transcripts and prevents ribosome pausing to enhance translational efficiency, an activity that influenza virus repurposes to promote its own replication (PMID:32839537). This RNA-binding activity is essential in vivo: an RNA-binding-deficient IFIT2 knock-in fails to protect mice from intranasal VSV, and IFIT2 acts specifically in neurons to block VSV neuropathogenesis through both peripheral-nerve and CNS routes (PMID:38888342, PMID:22615570, PMID:24991014). IFIT2 expression is driven downstream of interferon signaling via an IKKε→STAT1(Ser708) axis and through STAT1-dependent transcription, and its mRNA is destabilized by METTL3/YTHDF2-dependent m6A decay (PMID:22065572, PMID:35094011). IFIT2 additionally functions as a microtubule-associated protein enriched in the mitotic spindle (PMID:17030862), amplifies IFN-β/IRF3 signaling and inflammatory cytokine output in macrophages (PMID:24014876, PMID:24198415), suppresses NADPH oxidase by binding the p67phox subunit (PMID:29666281), and acts as a tumor suppressor by restraining aPKC-mediated epithelial-mesenchymal transition (PMID:22986528, PMID:29358655).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1996 Medium

    Established the molecular identity of IFIT2 as an interferon- and LPS-inducible protein built from tetratricopeptide repeats, framing it from the outset as a scaffold for protein-protein interactions.

    Evidence cDNA cloning, TPR sequence analysis, and northern blot induction in macrophages and 3T3 cells (murine GARG-39 homolog)

    PMID:8660659

    Open questions at the time
    • No biochemical demonstration of any binding partner at this stage
    • No functional antiviral or signaling assay
  2. 2006 Medium

    Showed IFIT2 has a cytoskeletal role independent of RNA biology, binding microtubules and localizing to the mitotic spindle.

    Evidence In vitro microtubule binding assay and immunofluorescence co-localization with β-tubulin

    PMID:17030862

    Open questions at the time
    • Functional consequence for mitosis not established
    • Relationship to RNA-binding function unclear
  3. 2008 Medium

    Defined a post-transcriptional immunomodulatory function whereby IFIT2 dampens inflammatory cytokine output by reducing mRNA stability.

    Evidence IFIT2-overexpressing RAW264.7 macrophages with actinomycin D mRNA stability chase and cytokine ELISA

    PMID:19108715

    Open questions at the time
    • Direct RNA binding not demonstrated here
    • 3' UTR determinants of susceptibility not defined at sequence level
    • Overexpression-based, no loss-of-function
  4. 2011 Medium

    Placed IFIT2 induction within a specific signaling axis, identifying IKKε-driven STAT1 Ser708 phosphorylation as required for IFIT2 expression and antiviral control of WNV.

    Evidence IKKε−/− and IFIT2−/− models, site-specific STAT1 phosphorylation analysis, CRM1 inhibition, WNV infection

    PMID:22065572

    Open questions at the time
    • Direct kinase-substrate biochemistry between IKKε and STAT1 Ser708 not reconstituted
    • Generality beyond WNV not tested
  5. 2012 High

    Established IFIT2 as a tissue- and virus-specific antiviral effector in the CNS and as a tumor suppressor restraining EMT, revealing two distinct disease-relevant roles.

    Evidence Intranasal VSV challenge of Ifit2−/− vs Ifit1−/− mice; IFIT2 shRNA knockdown and aPKC epistasis in oral squamous carcinoma cells with metastasis model

    PMID:22615570 PMID:22986528

    Open questions at the time
    • Molecular basis of CNS-specific protection not defined at this stage
    • Mechanism linking IFIT2 loss to aPKC activation unresolved
  6. 2013 Medium

    Distinguished IFIT2 as a positive regulator of interferon induction and inflammatory signaling, not merely a direct effector, by showing it promotes IFN-α/β and IRF3 activation in macrophages.

    Evidence Ifit2−/− macrophages with MHV coronavirus and LPS endotoxin models, IRF3 phosphorylation western blot, cytokine ELISA

    PMID:24014876 PMID:24198415

    Open questions at the time
    • Mechanism by which IFIT2 promotes IRF3 phosphorylation unknown
    • No direct molecular partner identified in this signaling step
  7. 2014 High

    Mapped IFIT2 antiviral protection to neuronal compartments by tracing blocked VSV spread from periphery to CNS in knockout mice.

    Evidence Subcutaneous footpad VSV infection of Ifit2−/− mice with anatomical viral titer dissection

    PMID:24991014

    Open questions at the time
    • Molecular mechanism of neuronal protection not yet established
    • Cell-intrinsic vs systemic contribution not resolved
  8. 2016 Medium

    Connected IFIT2 transcription to JAK-STAT pathway integrity, showing AJUBA-mediated JAK1 dissociation represses STAT1-dependent IFIT2 expression and apoptosis in cancer.

    Evidence Co-IP of AJUBA-JAK1, STAT1 phosphorylation and IFIT2 expression analysis, apoptosis assays in colorectal cancer cells

    PMID:27893714

    Open questions at the time
    • IFIT2's direct contribution to apoptosis not isolated from broader STAT1 program
    • Single-lab Co-IP
  9. 2017 Medium

    Reinforced IFIT2's tumor-suppressive role as a downstream effector of the PLZF/STAT1 growth-suppressive axis, and revealed its proteasome-coupled centrosomal aggregation behavior.

    Evidence PLZF overexpression with IFIT2 knockdown epistasis and xenograft; proteasome inhibition with colchicine/ciliobrevin perturbation of IFIT2 localization and apoptosis assay

    PMID:28367102 PMID:29358655

    Open questions at the time
    • Mechanism linking IFIT2 to growth suppression downstream of STAT1 incomplete
    • Functional role of centrosomal aggregation unclear
  10. 2018 Medium

    Resolved the architecture of the IFIT complex, showing IFIT2-IFIT3 heterodimers form preferentially and that IFIT3 stabilizes IFIT1 and enhances its translation-inhibitory activity, and identified IFIT2 as a p67phox-binding suppressor of NADPH oxidase.

    Evidence In vitro reconstitution of IFIT complexes with YxxxL mutagenesis and cap0 mRNA assays; Co-IP and ROS measurement in Ifit2-knockout leukocytes with C. albicans model

    PMID:29554348 PMID:29666281

    Open questions at the time
    • Structural basis of heterodimer formation not yet resolved at this stage
    • Mechanism of p67phox suppression beyond binding not defined
  11. 2020 High

    Defined IFIT2's RNA-binding specificity and dual translational role, showing it binds AU-rich mRNAs to prevent ribosome pausing and enhance translation—an activity hijacked by influenza.

    Evidence CLIP-seq, polysome and ribosome profiling, and CRISPR-Cas9 knockout during influenza infection

    PMID:32839537

    Open questions at the time
    • Reconciliation of translation enhancement with translation inhibition of short-UTR viral mRNAs not fully mechanistic
    • Structural basis of AU-rich recognition not resolved here
  12. 2022 Medium

    Identified an m6A-based decay mechanism controlling IFIT2 abundance, linking METTL3/YTHDF2 to destabilization of IFIT2 mRNA in cancer.

    Evidence METTL3 perturbation, MeRIP-seq, YTHDF2 rescue, and mRNA stability assays in intrahepatic cholangiocarcinoma

    PMID:35094011

    Open questions at the time
    • Generality of m6A control of IFIT2 across cell types untested
    • Single-lab
  13. 2024 High

    Provided definitive in vivo evidence that RNA binding is the essential antiviral activity of IFIT2 and that it acts cell-autonomously in neurons.

    Evidence RNA-binding mutant knock-in and neuron-specific conditional knockout mice challenged with VSV

    PMID:38888342

    Open questions at the time
    • Identity of the in vivo RNA targets driving protection not enumerated
    • Contribution of IFIT3 partnership in neurons not tested
  14. 2025 High

    Delivered the structural basis for IFIT2-IFIT3 antiviral recognition, showing a domain-swapped heterodimer that reads short 5' UTRs as a self/non-self discriminator independent of cap structure.

    Evidence 3.2 Å cryo-EM structure, in vitro translation inhibition, RNA binding to VEEV UTRs, and 5' UTR length-swap reporters

    PMID:40079162 PMID:41093992

    Open questions at the time
    • How short-UTR recognition is reconciled with AU-rich translation enhancement remains open
    • In vivo relevance of the structural mechanism to neuronal protection not directly linked

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how IFIT2's opposing translational activities—repression of short-UTR viral mRNAs within the IFIT2-IFIT3 complex versus enhancement of AU-rich mRNA translation—are mechanistically partitioned and regulated in vivo, and which RNA targets underlie its neuron-specific protection.
  • No unified model integrating translation-enhancing and translation-repressing activities
  • In vivo RNA targets in neurons not identified
  • Role of microtubule association relative to RNA functions unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 4 GO:0045182 translation regulator activity 3 GO:0008092 cytoskeletal protein binding 2 GO:0098772 molecular function regulator activity 1
Localization
GO:0005815 microtubule organizing center 1 GO:0005829 cytosol 1 GO:0005856 cytoskeleton 1
Pathway
R-HSA-168256 Immune System 4 R-HSA-1643685 Disease 3 R-HSA-8953854 Metabolism of RNA 3
Partners
IFIT1IFIT3P67PHOXTUBB
Complex memberships
IFIT2-IFIT3 heterodimer

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2025 Cryo-EM structure of the IFIT2-IFIT3 complex at 3.2 Å reveals a domain-swapped heterodimer; this complex recognizes viral mRNAs bearing short 5' UTRs (<50 nucleotides) as a molecular pattern for innate immune recognition, inhibits their translation, and confers antiviral activity against viruses such as VSV and parainfluenza virus 3. Cryo-EM structure determination (3.2 Å), in vitro translation inhibition assays, antiviral activity assays with recombinant viruses, 5' UTR length-swap reporter experiments Nature microbiology High 41093992
2025 IFIT2-IFIT3 heterodimer binds directly to the VEEV 3' UTR in vitro; 5' UTR lengths <50 nucleotides are necessary and sufficient for translation inhibition by the IFIT2-IFIT3 complex, independent of cap structure. In vitro RNA-binding assays, recombinant VEEV clones with swapped 3' UTRs, in vitro translation inhibition assays Journal of interferon & cytokine research Medium 40079162
2018 IFIT1 and IFIT3 interact via a YxxxL motif in their C-termini; IFIT2 and IFIT3 form homodimers that dissociate to yield a more stable IFIT2-IFIT3 heterodimer, which also associates with IFIT1. IFIT3 stabilizes IFIT1 protein expression, promotes IFIT1 binding to cap0 Zika virus reporter mRNA, and enhances IFIT1-mediated translation inhibition. In vitro reconstitution of IFIT complexes, cap0 mRNA-binding assay, translation inhibition assay, site-directed mutagenesis of YxxxL motif Nucleic acids research High 29554348
2020 IFIT2 binds directly to viral and cellular mRNAs in AU-rich regions (demonstrated by CLIP-seq during influenza virus infection); ribosome/polysome profiling showed that IFIT2 prevents ribosome pausing on bound mRNAs, thereby enhancing translational efficiency. Influenza virus repurposes this activity to promote viral mRNA translation and replication. CLIP-seq, polysome profiling, ribosome profiling, CRISPR-Cas9 knockout screen Nature microbiology High 32839537
2024 IFIT2 RNA-binding activity is essential for its antiviral function in vivo: mice expressing a mutant IFIT2 that cannot bind RNA were as susceptible to intranasal VSV infection as Ifit2−/− mice. Additionally, neuron-specific conditional knockout of Ifit2 was sufficient to render mice susceptible to VSV neuropathogenesis via the intranasal (but not subcutaneous) route, placing IFIT2 function specifically in neurons. Conditional knockout mouse (neuron-specific Cre), RNA-binding mutant knock-in mouse, intranasal/subcutaneous VSV challenge, survival and viral load analysis mBio High 38888342
2006 GARG39/IFIT2 protein interacts with microtubules in vitro, co-localizes with β-tubulin in vivo, and is enriched in the mitotic spindle of non-neuronal cells undergoing mitosis, identifying it as a microtubule-associated protein. In vitro microtubule binding assay, immunofluorescence co-localization with β-tubulin, mitotic cell imaging The Journal of general virology Medium 17030862
2017 Proteasome inhibition blocks IFIT2 degradation and causes IFIT2 to aggregate at the centrosome; this aggregation depends on intact microtubule dynamics (colchicine-sensitive) and dynein activity (ciliobrevin-sensitive). IFIT2 combined with proteasome inhibitor synergistically induces cancer cell apoptosis. Immunofluorescence localization after proteasome inhibitor treatment, pharmacological inhibition of microtubules (colchicine) and dynein (ciliobrevin), apoptosis assay International journal of biological sciences Medium 28367102
2018 IFIT2 is a binding partner of p67phox, a critical regulatory subunit of NADPH oxidase; loss of IFIT2 increases ROS production by leukocytes, suggesting IFIT2 suppresses NADPH oxidase activation. Ifit2 knockout mice showed improved survival and reduced fungal burden in a systemic Candida albicans model. Co-immunoprecipitation/binding partner identification, ROS measurement in Ifit2-knockout leukocytes, in vivo C. albicans infection survival study mBio Medium 29666281
2013 Ifit2 deficiency in macrophages impairs IFN-α/β mRNA and protein production following MHV coronavirus infection, placing Ifit2 as a positive regulator of IFN-α/β induction (rather than solely a direct antiviral effector) in macrophages during coronavirus CNS infection. Ifit2−/− mouse model, ex vivo microglia/macrophage analysis, IFN-α/β mRNA and protein quantification in bone marrow-derived macrophages, in vivo viral load measurement Journal of virology Medium 24198415
2011 IKKε phosphorylates STAT1 at serine 708, which is required for IFIT2 expression; loss of IKKε abrogates IFIT2 induction and enhances WNV replication. STAT1 tyrosine dephosphorylation and CRM1-mediated nuclear-cytoplasmic shuttling are prerequisites for Ser-708 phosphorylation. This defines an IFN-induced IKKε→STAT1(Ser708)→IFIT2 signaling axis. IKKε−/− and IFIT2−/− cell/mouse models, STAT1 phosphorylation analysis (site-specific antibodies), CRM1 inhibitor (leptomycin B) treatment, WNV infection assays The Journal of biological chemistry Medium 22065572
2013 IFIT2 promotes IRF3 phosphorylation, thereby amplifying LPS-induced IFN-β production and subsequent IL-6/TNF-α secretion (post-transcriptionally). Ifit2-deficient mice show reduced serum IL-6 and TNF-α and reduced mortality in endotoxin shock. IFIT2 expression is induced in an IFN-α receptor- and IRF9-dependent manner. Ifit2−/− mouse generation, bone marrow-derived macrophage cytokine assays (ELISA), IRF3 phosphorylation western blotting, LPS endotoxin shock model Journal of immunology Medium 24014876
2008 Overexpression of IFIT2 in macrophages selectively reduces LPS-induced TNF-α, IL-6, and MIP-2 expression by decreasing mRNA stability at the post-transcriptional level; characteristics of the 3' UTR of transcripts determine susceptibility to IFIT2-mediated regulation. Stable IFIT2-overexpressing RAW264.7 macrophage lines, mRNA stability assay (actinomycin D chase), ELISA, western blot BMC immunology Medium 19108715
2012 Depletion of IFIT2 in oral squamous cell carcinoma cells activates atypical PKC (aPKC), leading to epithelial-mesenchymal transition, enhanced cell migration, and invasiveness. Inhibition of aPKC (by pseudosubstrate or siRNA) abolished these phenotypes, placing aPKC downstream of IFIT2. Stable IFIT2 shRNA knockdown cell lines, aPKC pseudosubstrate inhibitor treatment, siRNA knockdown of aPKC, migration/invasion assays, tail-vein metastasis model Oncogene Medium 22986528
2016 AJUBA binds directly to the FERM domain of JAK1, dissociating JAK1 from the IFN-γ receptor and thereby inhibiting STAT1 phosphorylation and nuclear translocation, which represses IFIT2 gene expression and inhibits apoptosis in colorectal cancer cells. Co-immunoprecipitation (AJUBA-JAK1 binding), STAT1 phosphorylation assay, IFIT2 promoter/expression analysis, apoptosis assays, siRNA knockdown Oncogene Medium 27893714
2022 METTL3 mediates m6A modification of IFIT2 mRNA, which accelerates IFIT2 mRNA decay in a YTHDF2-dependent manner in intrahepatic cholangiocarcinoma. METTL3 transcription itself is driven by H3K4me3 activation. METTL3 knockdown/overexpression, MeRIP-seq (m6A mapping), YTHDF2 knockdown rescue experiments, mRNA stability assays, ChIP-seq for H3K4me3 Oncogene Medium 35094011
2012 Ifit2 is required for protection against lethal VSV neuropathogenesis following intranasal infection in mice. Ifit2−/− mice show several-hundred-fold higher VSV titers in the brain (without broadened cell tropism) compared to wild-type or Ifit1−/− mice, establishing Ifit2 as a tissue- and virus-specific ISG antiviral effector in the CNS. Ifit2−/− and Ifit1−/− knockout mouse infection models (intranasal VSV), viral titer quantification in CNS and peripheral organs, neurological phenotyping PLoS pathogens High 22615570
2014 Ifit2 protects peripheral nervous system neurons from VSV infection after subcutaneous footpad injection. In Ifit2−/− mice, VSV spread from lymph node to sciatic nerve, spinal cord, and brain causing paralysis, whereas in wild-type mice this neuronal spread was blocked despite equivalent viral replication in the lymph node. Ifit2−/− mouse model, subcutaneous footpad VSV injection, viral titer in lymph node, sciatic nerve, spinal cord, and brain, neurological outcome assessment Journal of virology High 24991014
2017 PLZF increases STAT1 protein levels, which drives IFIT2 mRNA transcription; ablation of IFIT2 in PLZF-overexpressing gallbladder cancer cells partially abrogates the tumor-suppressive effect of PLZF (reduced growth, EMT inhibition), placing IFIT2 downstream of PLZF/STAT1 in a growth-suppressive pathway. PLZF overexpression, IFIT2 shRNA knockdown in PLZF-OE cells (epistasis), STAT1 western blot, RT-PCR, xenograft mouse model Cell death & disease Medium 29358655
1996 GARG-39 (murine IFIT2 homolog) was identified as a protein containing 10 tetratricopeptide repeat (TPR) domains, induced by LPS and interferons (α/β and γ) in macrophages. The TPR domain architecture was predicted to mediate protein-protein interactions in multicomponent assemblies. cDNA cloning, sequence analysis of TPR domains, northern blot induction assays in 3T3 cells and macrophages with LPS/IFN stimulation Archives of biochemistry and biophysics Medium 8660659
2024 IFIT2 knockdown in foamy macrophages attenuates ox-LDL-induced iron retention (reduces ferritin-L, ferritin-H, Fe2+ and Fe3+) and promotes cholesterol efflux via upregulation of PPARγ/LXRα/ABCA1/ABCG1 pathway; in vivo Ifit2 knockdown in atherosclerotic APOE−/− mice reduces plaque area and lipid accumulation. shRNA lentivirus knockdown in HFD-induced APOE−/− mice (in vivo), ox-LDL-induced foamy macrophage model (in vitro), western blot for iron/cholesterol efflux proteins, iron measurement International immunopharmacology Medium 39276454
2020 IFIT2 overexpression in K562 chronic myeloid leukemia cells inhibits cell proliferation and causes G1 arrest by inhibiting the BCR-ABL oncoprotein and its downstream AKT/mTOR signaling, and by inducing p27kip1 via degradation of cullin1-mediated E3 ligases. Stable IFIT2-overexpressing K562 cell line, cell cycle analysis, western blot for BCR-ABL, p-AKT, p-mTOR, p27kip1, and cullin1 International journal of molecular medicine Low 32124954
2025 IRF1 directly binds to the IFIT2 gene promoter and activates its transcription, as validated by promoter-reporter (luciferase) assay and protein-binding microarray mapping of IRF1 binding motifs. Luciferase reporter assay with IFIT2 promoter constructs, protein-binding microarray, IRF1 knockout and overexpression in HeLa cells, RNA-seq bioRxiv (preprint)preprint Low
2024 Biophysical characterization shows that cap-adjacent m6Am modification on RNA strongly blocks formation of the IFIT protein complex with RNA; the IFIT2-IFIT3 interaction (and IFIT1-IFIT3 interaction at nanomolar affinity) was characterized with kinetic parameters. m6A within the 5'UTR (not at cap) is not recognized by IFIT proteins and does not contribute to translation repression. Biophysical binding assays (SPR/ITC-type kinetics), RNA with defined 5'-cap modifications, IFIT protein complex reconstitution bioRxiv (preprint)preprint Low

Source papers

Stage 0 corpus · 58 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2012 Interferon-induced Ifit2/ISG54 protects mice from lethal VSV neuropathogenesis. PLoS pathogens 155 22615570
2016 Long non-coding RNA LINC00161 sensitises osteosarcoma cells to cisplatin-induced apoptosis by regulating the miR-645-IFIT2 axis. Cancer letters 121 27609068
2006 Coordinated regulation and widespread cellular expression of interferon-stimulated genes (ISG) ISG-49, ISG-54, and ISG-56 in the central nervous system after infection with distinct viruses. Journal of virology 113 17079283
2022 METTL3 promotes intrahepatic cholangiocarcinoma progression by regulating IFIT2 expression in an m6A-YTHDF2-dependent manner. Oncogene 108 35094011
2018 IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA. Nucleic acids research 90 29554348
2020 Influenza virus repurposes the antiviral protein IFIT2 to promote translation of viral mRNAs. Nature microbiology 76 32839537
2013 A role for Ifit2 in restricting West Nile virus infection in the brain. Journal of virology 71 23740986
2013 A death-promoting role for ISG54/IFIT2. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 67 23570386
2019 Baicalein Suppresses Stem Cell-Like Characteristics in Radio- and Chemoresistant MDA-MB-231 Human Breast Cancer Cells through Up-Regulation of IFIT2. Nutrients 63 30875792
2016 The LIM protein AJUBA promotes colorectal cancer cell survival through suppression of JAK1/STAT1/IFIT2 network. Oncogene 61 27893714
1996 The glucocorticoid attenuated response genes GARG-16, GARG-39, and GARG-49/IRG2 encode inducible proteins containing multiple tetratricopeptide repeat domains. Archives of biochemistry and biophysics 57 8660659
2011 Inhibitor of κB kinase epsilon (IKK(epsilon)), STAT1, and IFIT2 proteins define novel innate immune effector pathway against West Nile virus infection. The Journal of biological chemistry 55 22065572
2013 IFIT2 is an effector protein of type I IFN-mediated amplification of lipopolysaccharide (LPS)-induced TNF-α secretion and LPS-induced endotoxin shock. Journal of immunology (Baltimore, Md. : 1950) 54 24014876
2012 Depleting IFIT2 mediates atypical PKC signaling to enhance the migration and metastatic activity of oral squamous cell carcinoma cells. Oncogene 54 22986528
1998 IFI60/ISG60/IFIT4, a new member of the human IFI54/IFIT2 family of interferon-stimulated genes. Genomics 53 9828129
2013 Ifit2 deficiency results in uncontrolled neurotropic coronavirus replication and enhanced encephalitis via impaired alpha/beta interferon induction in macrophages. Journal of virology 49 24198415
2008 Forced IFIT-2 expression represses LPS induced TNF-alpha expression at posttranscriptional levels. BMC immunology 49 19108715
2015 Blocking TNF-α inhibits angiogenesis and growth of IFIT2-depleted metastatic oral squamous cell carcinoma cells. Cancer letters 45 26515391
2018 PLZF inhibits proliferation and metastasis of gallbladder cancer by regulating IFIT2. Cell death & disease 38 29358655
2017 Ifit2 Is a Restriction Factor in Rabies Virus Pathogenicity. Journal of virology 38 28637751
2014 Interferon-induced protein Ifit2 protects mice from infection of the peripheral nervous system by vesicular stomatitis virus. Journal of virology 37 24991014
2014 MicroRNA-645, up-regulated in human adencarcinoma of gastric esophageal junction, inhibits apoptosis by targeting tumor suppressor IFIT2. BMC cancer 36 25174799
2014 Sendai virus pathogenesis in mice is prevented by Ifit2 and exacerbated by interferon. Journal of virology 36 25231314
2017 Long non-coding RNA00364 represses hepatocellular carcinoma cell proliferation via modulating p-STAT3-IFIT2 signaling axis. Oncotarget 30 29254221
2022 METTL3/m6A/IFIT2 regulates proliferation, invasion and immunity in esophageal squamous cell carcinoma. Frontiers in pharmacology 27 36386128
2017 Curcumin induces apoptosis in human leukemic cell lines through an IFIT2-dependent pathway. Cancer biology & therapy 27 28071969
2017 Inhibition of Proteasome Activity Induces Aggregation of IFIT2 in the Centrosome and Enhances IFIT2-Induced Cell Apoptosis. International journal of biological sciences 27 28367102
2012 Crystal structure of IFIT2 (ISG54) predicts functional properties of IFITs. Cell research 27 22964712
2020 Ifit2 deficiency restricts microglial activation and leukocyte migration following murine coronavirus (m-CoV) CNS infection. PLoS pathogens 25 33253295
2006 Identification of Japanese encephalitis virus-inducible genes in mouse brain and characterization of GARG39/IFIT2 as a microtubule-associated protein. The Journal of general virology 25 17030862
2017 Decreased expression of interferon-induced protein 2 (IFIT2) by Wnt/β-catenin signaling confers anti-apoptotic properties to colorectal cancer cells. Oncotarget 21 29245969
2018 Pathogenic Effects of IFIT2 and Interferon-β during Fatal Systemic Candida albicans Infection. mBio 20 29666281
2022 LncRNA RP5-998N21.4 promotes immune defense through upregulation of IFIT2 and IFIT3 in schizophrenia. Schizophrenia (Heidelberg, Germany) 14 35232977
2021 Super-enhancer-associated long noncoding RNA RP11-569A11.1 inhibited cell progression and metastasis by regulating IFIT2 in colorectal cancer. Journal of clinical laboratory analysis 13 33942366
2023 MiR-199a-5P promotes osteogenic differentiation of human stem cells from apical papilla via targeting IFIT2 in apical periodontitis. Frontiers in immunology 11 37063854
2022 IFIT2-depleted metastatic oral squamous cell carcinoma cells induce muscle atrophy and cancer cachexia in mice. Journal of cachexia, sarcopenia and muscle 10 35170238
2024 The interferon-induced protein, IFIT2, requires RNA-binding activity and neuronal expression to protect mice from intranasal vesicular stomatitis virus infection. mBio 9 38888342
2022 STAT1/IFIT2 signaling pathway is involved in PD-L1-mediated epithelial-to-mesenchymal transition in human esophageal cancer. Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 9 35107757
2020 SAHA Overcomes 5-FU Resistance in IFIT2-Depleted Oral Squamous Cell Carcinoma Cells. Cancers 9 33256074
2023 IFIT2 Depletion Promotes Cancer Stem Cell-like Phenotypes in Oral Cancer. Biomedicines 8 36979874
2020 Overexpression of IFIT2 inhibits the proliferation of chronic myeloid leukemia cells by regulating the BCR‑ABL/AKT/mTOR pathway. International journal of molecular medicine 8 32124954
2025 The IFIT2-IFIT3 antiviral complex targets short 5' untranslated regions on viral mRNAs for translation inhibition. Nature microbiology 7 41093992
2015 Cox4i2, Ifit2, and Prdm11 Mutant Mice: Effective Selection of Genes Predisposing to an Altered Airway Inflammatory Response from a Large Compendium of Mutant Mouse Lines. PloS one 7 26263558
2024 IFIT2 mediates iron retention and cholesterol efflux in atherosclerosis. International immunopharmacology 6 39276454
2023 Ifit2 restricts murine coronavirus spread to the spinal cord white matter and its associated myelin pathology. Journal of virology 6 37504572
2023 IRF7 and IFIT2 in mediating different hemorrhage outcomes for non-small cell lung cancer after bevacizumab treatment. Journal of thoracic disease 5 37197507
2021 Long Non-Coding RNA GRIK1-AS1 Inhibits the Proliferation and Invasion of Gastric Cancer Cells by Regulating the miR-375/IFIT2 Axis. Frontiers in oncology 4 34660323
2025 Sequence Diversity in the 3' Untranslated Region of Alphavirus Modulates IFIT2-Dependent Restriction in a Cell Type-Dependent Manner. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 3 40079162
2025 IFIT2-induced transcriptomic changes in Mycobacterium tuberculosis infected macrophages. Frontiers in cellular and infection microbiology 3 40463504
2020 Clinical significance of IFIT2 expression in human renal cancer tissues. Translational cancer research 3 35117687
2018 Molecular identification and transcriptional regulation of porcine IFIT2 gene. Molecular biology reports 3 29623507
2018 A haplotype variant of porcine IFIT2 increases poly(I:C)-induced activation of NF-κB and ISRE-binding factors. Molecular biology reports 2 30298349
2025 Short 5' UTRs serve as a marker for viral mRNA translation inhibition by the IFIT2-IFIT3 antiviral complex. bioRxiv : the preprint server for biology 1 39990370
2025 Correlation Analysis Between IFIT2 and HLA Class II in Oral Cancer. Anticancer research 1 40295076
2025 Therapeutic potential of IFIT2 in human diseases. Cytokine 1 41046825
2026 SPIB suppresses protective autophagy via the IFIT2/PINK1/Parkin axis to promote anoikis in colorectal cancer. Cancer cell international 0 41572316
2026 IL-6 as a driver of bone invasion in IFIT2-depleted oral squamous cell carcinoma. Cancer immunology, immunotherapy : CII 0 41619016
2026 Cross-Cohort Transcriptomic Integration Identifies IFIT2 as a Translational Diagnostic Biomarker and Functional Driver of Inflammation-Linked Tubular Injury in Chronic Kidney Disease. Human mutation 0 42147814

Missed literature

Know a paper Affinage missed for IFIT2? Flag it for the maintainers and the community.

No submissions yet.