Affinage

HOXB3

Homeobox protein Hox-B3 · UniProt P14651

Length
431 aa
Mass
44.3 kDa
Annotated
2026-06-10
43 papers in source corpus 23 papers cited in narrative 23 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

HOXB3 is a homeodomain transcription factor that patterns the vertebrate hindbrain, governs hematopoietic stem and progenitor cell behavior, and acts as a context-dependent oncogenic driver by directly binding DNA and regulating downstream target genes (PMID:9052833, PMID:19854132, PMID:21320481). During hindbrain development, Hoxb3 expression is established by direct binding of the Maf/bZIP factor kreisler/Krml1 to conserved blocks in an r5 enhancer, with Krox20 synergizing to restrict expression to rhombomere 5, while a separate set of modular cis-elements directs neural, neural crest, and mesodermal domains (PMID:9144291, PMID:11823429, PMID:11254356); one of these (b3IIIa) is a vagal neural-crest enhancer relevant to enteric nervous system colonization (PMID:15768390). HOXB3 participates in cross-regulatory Hox networks, binding its own and paralog loci—repressing Hoxb1 through a discrete S3 site and being bound at element IIIa by both Hoxb3 and Hoxb4 (PMID:12482716, PMID:21320481). Its expression boundaries are maintained post-establishment by the Polycomb-group protein rae28 (PMID:11044623). In hematopoiesis, HOXB3 promotes progenitor proliferation: overexpression drives myeloproliferation and impairs lymphoid development, Hoxb3/Hoxb4 double-knockout mice show a reduced and slow-cycling HSC pool, and Hoxb3-deficient mice develop age-dependent defects in IL-7-dependent B lymphopoiesis (PMID:9052833, PMID:12748289, PMID:17309827). In cancer, HOXB3 acts oncogenically by transcriptionally activating DNMT3B, which silences the RASSF1A tumor suppressor and miR-375 (a self-sustaining feedback loop), and by activating CDCA3, Notch1, and WNT pathway genes to drive proliferation, migration, and invasion (PMID:19854132, PMID:23219899, PMID:29439669, PMID:37652291); in castration-resistant prostate cancer, WNT3A and APC loss release HOXB3 from the destruction complex to enter the nucleus and activate WNT targets (PMID:36973255). Transforming activity requires cooperation with PBX1 via its conserved tetrapeptide and homeodomain (PMID:9692548). HOXB3 activity is further tuned by post-translational control—STUB1-mediated ubiquitination and degradation (with HOXB3 activating PARK7 to regulate ferroptosis) and SIRT5-mediated desuccinylation (PMID:39501077, PMID:39145876)—and in glioblastoma it forms phase-separated condensates through its intrinsically disordered regions and RUNX1 interaction that drive super-enhancer-associated oncogenic transcription (PMID:41028837).

Mechanistic history

Synthesis pass · year-by-year structured walk · 23 steps
  1. 1997 High

    Established that HOXB3 directly controls hematopoietic proliferation and lineage output, defining its physiological role in blood formation.

    Evidence Retroviral overexpression in murine bone marrow with transplantation, flow cytometry and colony assays

    PMID:9052833

    Open questions at the time
    • Did not identify direct transcriptional targets mediating the proliferative effect
    • Gain-of-function may not reflect endogenous dosage
  2. 1997 High

    Identified the upstream activator of Hoxb3 in the hindbrain, showing kreisler/Krml1 directly binds the r5 enhancer to drive segmental expression.

    Evidence Transgenic reporter analysis with in vitro binding and enhancer mutagenesis

    PMID:9144291

    Open questions at the time
    • Did not establish the full combinatorial logic restricting expression to r5
    • Role of the required Ets site not assigned to a specific factor
  3. 1999 High

    Confirmed kreisler as a conserved direct regulator of Hox group 3 paralogs and showed enhancer architecture determines distinct segmental boundaries.

    Evidence Transgenic, deletion, ectopic expression and kreisler-null analysis of Hoxa3/Hoxb3 enhancers

    PMID:9895323

    Open questions at the time
    • Did not address downstream targets of group 3 Hox in r5/r6
  4. 1998 High

    Demonstrated HOXB3 transforming activity depends on cofactor PBX1, defining a cooperative DNA-binding requirement for oncogenic function.

    Evidence Rat-1 transformation assay with tetrapeptide/homeodomain mutagenesis and PBX1 modulation

    PMID:9692548

    Open questions at the time
    • Did not identify the target genes through which HOXB3/PBX1 drive transformation
  5. 1998 Medium

    Provided early evidence that HOXB3 binds defined TAATTA motifs to activate a developmental target gene (Otx2).

    Evidence Reporter assays and EMSA with recombinant HOXB3 homeodomain in embryonal carcinoma cells

    PMID:9556594

    Open questions at the time
    • In vivo relevance of Otx2 regulation not established
    • Single lab, limited functional validation
  6. 2000 Medium

    Distinguished establishment from maintenance of Hoxb3 boundaries, assigning Polycomb (rae28) to long-term domain restriction.

    Evidence rae28-knockout mouse with in situ hybridization and temporal expression analysis

    PMID:11044623

    Open questions at the time
    • Mechanism of rae28 recruitment to Hoxb3 not defined
    • Single lab
  7. 2001 Medium

    Mapped the modular cis-regulatory architecture of the Hoxb3 locus, assigning separable elements to distinct tissue/stage expression domains.

    Evidence Transgenic lacZ reporter dissection of multiple DNA fragments alone and in combination

    PMID:11254356

    Open questions at the time
    • Trans-acting factors for several elements not identified
    • Single lab
  8. 2002 High

    Defined the combinatorial logic restricting Hoxb3 to r5, showing Krox20 synergizes with kreisler at the r5 enhancer.

    Evidence Krox20-null genetics, ectopic expression, enhancer mutagenesis and EMSA

    PMID:11823429

    Open questions at the time
    • Did not resolve how synergy is biochemically achieved at the enhancer
  9. 2002 Medium

    Revealed Hox auto/cross-regulation, with Hoxb3 and Hoxb4 binding element IIIa to control neural-tube expression independent of kreisler sites.

    Evidence Transgenic analysis with EMSA and deletion/mutational dissection of element IIIa

    PMID:12482716

    Open questions at the time
    • Direct autoregulatory link is indirect
    • Identity of GCCAGGC-binding factor unknown
  10. 2003 High

    Showed Hoxb3 and Hoxb4 cooperate to sustain the HSC pool through proliferation/self-renewal rather than lineage commitment.

    Evidence Double-knockout mice with competitive repopulation, colony assays, cell-cycle kinetics

    PMID:12748289

    Open questions at the time
    • Direct transcriptional targets in HSCs not identified
    • Individual contribution of Hoxb3 vs Hoxb4 not separated
  11. 2005 Medium

    Identified the b3IIIa element as a vagal neural-crest enhancer with relevance to enteric nervous system colonization.

    Evidence Transgenic lacZ reporter with cellular markers and Dom (Hirschsprung model) genetic cross

    PMID:15768390

    Open questions at the time
    • Causal role of HOXB3 itself in Hirschsprung phenotype not established
    • Single lab
  12. 2007 High

    Established a non-redundant role for endogenous Hoxb3 in IL-7-dependent B lymphopoiesis, refining its hematopoietic function.

    Evidence Hoxb3 knockout mice with flow cytometry, IL-7R analysis and IL-7 proliferation assays

    PMID:17309827

    Open questions at the time
    • Whether Hoxb3 directly regulates IL-7R transcription not shown
  13. 2009 High

    Defined an oncogenic epigenetic mechanism whereby HOXB3 activates DNMT3B to silence the RASSF1A tumor suppressor.

    Evidence Genome-wide shRNA screen, ChIP, bisulfite sequencing, rescue and xenograft experiments

    PMID:19854132

    Open questions at the time
    • Did not address context-dependent tumor-suppressive roles of HOXB3
  14. 2011 High

    Demonstrated direct Hoxb3-mediated repression of Hoxb1 via a discrete site, extending cross-regulatory Hox network function in the hindbrain.

    Evidence EMSA, in vivo ChIP, luciferase reporter and gain-of-function transgenic mouse

    PMID:21320481

    Open questions at the time
    • Cofactors at the S3 site not defined
  15. 2012 Medium

    Identified CDCA3 as a direct HOXB3 target driving prostate cancer proliferation and migration.

    Evidence ChIP, shRNA, overexpression, xenograft and proliferation/migration assays

    PMID:23219899

    Open questions at the time
    • Single lab
    • Generality across tumor types not established at this stage
  16. 2015 Medium

    Revealed a tumor-suppressive facet of HOXB3, antagonizing FLT3-ITD oncogenic signaling in pro-B cells.

    Evidence Overexpression in mouse pro-B cells with phospho-signaling Western blots and apoptosis/colony assays

    PMID:26482852

    Open questions at the time
    • No direct molecular link between HOXB3 and FLT3 pathway demonstrated
    • Single lab
  17. 2018 Medium

    Connected HOXB3 oncogenic outputs into a self-sustaining DNMT3B-miR-375 feedback loop in leukemia.

    Evidence Luciferase 3'-UTR reporter, bisulfite sequencing, shRNA, colony and xenograft assays

    PMID:29439669

    Open questions at the time
    • Single lab
    • Relative contribution of CDCA3 vs DNMT3B arms not quantified
  18. 2021 Medium

    Showed Hoxb3 directly regulates Jag1 in pharyngeal epithelium, linking it to neural crest migration via Notch ligand control.

    Evidence Binding assay at Jag1 site S2, gain-of-function transgenic mouse, in situ hybridization, migration analysis

    PMID:33505317

    Open questions at the time
    • Single lab
    • Endogenous loss-of-function effect on Jag1 not tested
  19. 2023 Medium

    Placed HOXB3 within WNT signaling as a destruction-complex-regulated transcription factor in castration-resistant prostate cancer.

    Evidence RNA-seq, WNT3A treatment, APC knockdown, nuclear fractionation and xenograft assays

    PMID:36973255

    Open questions at the time
    • Direct WNT target genes activated by HOXB3 not individually validated
    • Single lab
  20. 2023 Medium

    Identified Notch1 as a direct HOXB3 target driving Wnt/β-catenin-dependent trophoblast proliferation and invasion.

    Evidence Luciferase reporter, ChIP, siRNA rescue and invasion assays plus a rat preeclampsia model

    PMID:37652291

    Open questions at the time
    • Single lab
    • In vivo human relevance limited
  21. 2024 Medium

    Established post-translational control of HOXB3 stability by STUB1 and a downstream PARK7 axis governing ferroptosis and drug resistance.

    Evidence Co-IP, dual luciferase, knockdown/overexpression rescue and xenograft in ovarian cancer

    PMID:39501077

    Open questions at the time
    • Ubiquitination sites on HOXB3 not mapped
    • Single lab
  22. 2024 Medium

    Identified SIRT5-mediated desuccinylation as a suppressive post-translational modification of HOXB3 activity in trophoblasts.

    Evidence Co-IP, dual-luciferase, siRNA rescue and invasion/migration assays in HTR-8/SVneo cells

    PMID:39145876

    Open questions at the time
    • Specific desuccinylated residues not defined
    • Single lab
  23. 2025 High

    Revealed that HOXB3 can drive oncogenic transcription through RUNX1-dependent phase-separated condensates at super-enhancers, offering a targetable mechanism.

    Evidence Single-cell CUT&Tag, condensate analysis, peptide disruption and patient-derived xenografts in glioblastoma

    PMID:41028837

    Open questions at the time
    • Generality of condensate behavior beyond GBM subset unknown
    • How condensate formation integrates with cofactors like PBX1 not addressed

Open questions

Synthesis pass · forward-looking unresolved questions
  • The molecular basis determining whether HOXB3 acts as an oncogenic driver versus a tumor suppressor in a given cellular context remains unresolved.
  • No unifying model reconciling oncogenic (DNMT3B/WNT/CDCA3) and suppressive (FLT3-ITD) outputs
  • Cofactor and PTM combinations that switch HOXB3 output not systematically defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 5 GO:0003677 DNA binding 4
Localization
GO:0005634 nucleus 1
Pathway
R-HSA-1266738 Developmental Biology 5 R-HSA-74160 Gene expression (Transcription) 4 R-HSA-162582 Signal Transduction 3 R-HSA-1643685 Disease 3
Partners
PBX1RUNX1SIRT5STUB1

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 HOXB3 overexpression in murine bone marrow cells via retroviral gene transfer caused defective lymphoid development (reduced CD4+CD8+ thymocytes, absent IL-7-responsive pre-B progenitors) and progressive myeloproliferation (elevated granulocyte macrophage colony-forming cells), establishing a direct role for HOXB3 in hematopoietic proliferation and differentiation. Retroviral gene transfer into murine bone marrow; transplantation into mice; flow cytometry and colony assays Immunity High 9052833
1997 The kreisler protein (Krml1), a Maf/bZIP transcription factor expressed in rhombomeres 5 and 6, directly binds two conserved sequence blocks (19 bp and 45 bp) in the Hoxb3 r5 enhancer and activates Hoxb3 transcription in r5, with an Ets-related activation site also required for both activation and r5 restriction. Transgenic reporter analysis; in vitro binding assays; deletion/mutational analysis of enhancer elements Nature High 9144291
1999 Hoxa3, like its paralog Hoxb3, is a direct transcriptional target of kreisler (Krml1) in the hindbrain; a single high-affinity Krml1 binding site in the Hoxa3 r5/r6 enhancer is necessary and sufficient for kreisler-dependent activation, but the number and arrangement of binding sites differ between Hoxa3 and Hoxb3 enhancers, accounting for their distinct segmental restriction (r5/r6 vs. r5 only). Transgenic analysis; deletion analysis; ectopic kreisler expression; kreisler-null embryo analysis Development High 9895323
1998 HOXB3-induced cellular transformation and proliferation of Rat-1 cells requires cooperation with PBX1; the conserved tetrapeptide (mediating PBX binding) and homeodomain (mediating DNA binding) are both required for transforming activity, and modulating PBX1 levels strongly modulates HOXB3 transforming capacity. Rat-1 cell transformation assay; co-transfection with PBX1; deletion/point mutations of tetrapeptide and homeodomain Oncogene High 9692548
1998 HOXB1, HOXB2, and HOXB3 proteins all positively regulate a 534-bp upstream fragment of the Otx2 gene in embryonal carcinoma cells; HOXB3 (homeodomain-containing bacterial extract) binds to palindromic TAATTA sites within this fragment, and this binding region is necessary for Otx2 up-regulation. Co-transfection reporter assay; EMSA with recombinant HOXB3 homeodomain; nuclear extract binding assays Journal of Biological Chemistry Medium 9556594
2002 Krox20 (expressed in r3 and r5) is required for activity of the Hoxb3 r5 enhancer (but not the Hoxa3 r5/r6 enhancer); Krox20 binds the Hoxb3 r5 enhancer and synergizes with kreisler to restrict expression to r5 (the overlap domain of both factors), as shown by genetic analysis in Krox20-null embryos and ectopic Krox20 expression. Genetic analysis (Krox20-null mice); ectopic expression; mutational analysis of enhancer; EMSA EMBO Journal High 11823429
2001 Multiple widely-spaced cis-acting regulatory elements (Ib, IIIa, IVa, IVb, Va) within and flanking the Hoxb3 locus control distinct tissue- and stage-specific expression domains in a modular, additive manner; element IVa (r5 enhancer) controls anterior neural expression in r5, element IIIa directs anterior spinal cord/hindbrain to r6, region A/element I mediates posterior neural expression, and element Va controls mesoderm-specific expression. Transgenic lacZ reporter analysis with multiple DNA fragments; combinatorial element testing Developmental Biology Medium 11254356
2002 Hoxb3 auto/cross-regulates its own expression in the neural tube: within the 482-bp element IIIa, two Hox binding sites are bound in vitro by both Hoxb3 and Hoxb4 proteins; a novel GCCAGGC motif is also required; kreisler binding sites in this element are not required for r6 or neural crest expression. Transgenic analysis; in vitro binding (EMSA); deletion/mutational analysis of element IIIa Developmental Biology Medium 12482716
2003 Mice deficient in both Hoxb3 and Hoxb4 display reduced hematopoietic stem cell (HSC) pool in fetal liver, diminished progenitor numbers, impaired proliferative capacity of Lin-Sca1+c-kit+ progenitors in vitro, reduced in vivo repopulating capability, and slower cell cycle kinetics without perturbing lineage commitment, indicating that Hoxb3 and Hoxb4 cooperate to regulate HSC proliferation and self-renewal. Double-knockout mouse model; competitive repopulation assay; in vitro colony assay; flow cytometry; cytostatic drug treatment Molecular and Cellular Biology High 12748289
2000 The Polycomb group protein rae28 is required for maintenance (but not establishment) of Hoxb3 expression boundaries; rae28-deficient mice show ectopic anterior extension of Hoxb3 expression in hindbrain and pharyngeal arch despite normal kreisler and Krox20 expression, indicating rae28 restricts Hoxb3 expression domain post-establishment. rae28-knockout mouse analysis; in situ hybridization for Hoxb3, kreisler, Krox20, p75; temporal expression analysis Mechanisms of Development Medium 11044623
2005 The Hoxb3 cis-element b3IIIa functions as a vagal neural crest-specific enhancer that directs expression in vagal (but not trunk or sacral) neural crest, marks a subset of enteric neuroblasts, and is maintained in differentiated myenteric plexus neurons; in Dom mutant mice (Hirschsprung's model), colonization of b3IIIa-expressing cells in large intestine was incomplete. Transgenic lacZ reporter analysis; cellular marker co-localization; genetic cross with Dom mutant mice Developmental Dynamics Medium 15768390
2007 Hoxb3-deficient mice develop age-dependent impairment of B lymphopoiesis: by 6 months, Hoxb3-/- mice show reduced BM cellularity, twofold reduction in B220+CD43+ progenitor B cells, fivefold reduction in B220+CD43-IgM- precursor B cells, reduced IL-7 receptor expression on progenitor B cells, and impaired IL-7-driven proliferation in vitro. Hoxb3 knockout mouse analysis; flow cytometry; in vitro IL-7 stimulation proliferation assay; IL-7R expression analysis Experimental Hematology High 17309827
2009 HOXB3 epigenetically silences RASSF1A tumor suppressor by binding to and transcriptionally activating the DNMT3B promoter; DNMT3B is then recruited to the RASSF1A promoter via interactions with Polycomb repressor complex 2 and MYC, resulting in hypermethylation and silencing of RASSF1A; HOXB3 oncogenic activity in xenograft models is partly dependent on RASSF1A silencing. Genome-wide shRNA screen; ChIP; bisulfite sequencing; reporter assays; shRNA knockdown; mouse xenograft experiments Molecular Cell High 19854132
2011 Hoxb3 directly represses Hoxb1 transcription by binding to a novel site S3 on the Hoxb1 locus; EMSA confirmed protein-DNA interaction in vitro; in vivo ChIP in P19 cells and hindbrain tissues from Hoxb3(Tg) mutant confirmed occupancy; luciferase reporter assay in chick demonstrated transcriptional suppression; in wildtype E10.5 caudal hindbrain (where Hoxb1 is absent), Hoxb3 occupies the S3 site. EMSA; in vivo ChIP; luciferase reporter assay (chick in ovo); gain-of-function transgenic mouse Developmental Biology High 21320481
2012 HoxB3 promotes prostate cancer cell proliferation and migration by transcriptionally activating CDCA3; ChIP analysis showed HoxB3 binds the CDCA3 promoter; depletion of HoxB3 decreased proliferation in a CDCA3-dependent manner both in vitro and in vivo. ChIP; shRNA knockdown; overexpression; xenograft in vivo; proliferation/migration assays Cancer Letters Medium 23219899
2015 HOXB3 overexpression in mouse pro-B cells decreased FLT3-ITD-dependent cell proliferation and colony formation, increased apoptosis, and significantly reduced FLT3-ITD-induced phosphorylation of AKT, ERK, p38, and STAT5, indicating HOXB3 acts as a suppressor of FLT3-ITD oncogenic signaling. Overexpression in mouse pro-B cells; colony formation assay; Western blot for phospho-AKT, phospho-ERK, phospho-p38, phospho-STAT5; apoptosis assay Biochemical and Biophysical Research Communications Medium 26482852
2018 HOXB3 drives leukemogenesis partly by transcriptionally activating CDCA3 (confirmed by knockdown reducing CDCA3 and cell proliferation) and inducing DNMT3B expression; DNMT3B then methylates the pre-miR-375 promoter to silence miR-375, creating a HOXB3→DNMT3B→miR-375 feedback loop that sustains HOXB3 expression; miR-375 directly targets HOXB3 3'-UTR (luciferase reporter). Luciferase reporter assay for miR-375/HOXB3 3'-UTR; shRNA knockdown; colony formation; xenograft; bisulfite sequencing of pre-miR-375 promoter BMC Cancer Medium 29439669
2021 Hoxb3 directly regulates Jag1 expression in pharyngeal epithelium: molecular experiments showed Hoxb3 binds to an upstream genomic site S2 on the Jag1 locus; ectopic Hoxb3 in Hoxb3(Tg) mutant specifically upregulated Jag1 in ectodermal pharyngeal epithelial cells of PA2; elevated Jag1 led to abnormal cellular interaction and deficiency of neural crest cells migrating into PA2. ChIP/molecular binding assay; gain-of-function transgenic mouse; in situ hybridization; cellular migration analysis Frontiers in Physiology Medium 33505317
2023 In castration-resistant prostate cancer (CRPC), WNT3A signaling and APC deficiency cause HOXB3 to dissociate from the destruction complex and translocate to the nucleus, where it transcriptionally activates multiple WNT pathway genes; HOXB3 suppression reduced proliferation in APC-downregulated CRPC cells and re-sensitized APC-deficient CRPC xenografts to abiraterone. RNA sequencing; WNT3A treatment; APC knockdown; nuclear fractionation/localization; xenograft assays; proliferation assays Cell Death & Disease Medium 36973255
2023 HOXB3 transcriptionally activates Notch1 by binding to its promoter (confirmed by luciferase reporter and ChIP assays); this promotes Wnt/β-catenin pathway activation and trophoblast cell proliferation, migration, and invasion; Notch1 knockdown abrogated HOXB3 functions in trophoblasts. Luciferase reporter assay; ChIP; siRNA knockdown; cell proliferation/migration/invasion assays; rat PE model European Journal of Pharmacology Medium 37652291
2024 STUB1 (an E3 ubiquitin ligase) mediates ubiquitination and degradation of HOXB3; HOXB3 in turn transcriptionally activates PARK7 by binding to its promoter; the STUB1→HOXB3→PARK7 axis regulates ferroptosis and paclitaxel resistance in ovarian cancer cells. Co-immunoprecipitation; dual luciferase reporter assay; RT-qPCR; western blot; overexpression/knockdown rescue experiments; xenograft Communications Biology Medium 39501077
2024 SIRT5 desuccinylates HOXB3 as a post-translational modification, suppressing HOXB3 activity; SIRT5 inhibition increases trophoblast proliferation, invasion, and migration through HOXB3-dependent Notch and β-catenin signaling pathway activation; silencing HOXB3 reversed these effects. Co-immunoprecipitation; dual-luciferase reporter assay; siRNA knockdown rescue; proliferation/invasion/migration assays in HTR-8/SVneo cells Journal of Assisted Reproduction and Genetics Medium 39145876
2025 HOXB3 forms phase-separated condensates in a subset of glioblastoma cells through its intrinsically disordered regions and interactions with RUNX1; these condensates drive oncogenic transcription at super-enhancer-associated sites; a peptide (P621-R9) disrupting HOXB3 condensation altered chromatin structure, reduced super-enhancer-driven transcription, and selectively diminished tumorigenic potential in GBM patient-derived xenograft models with HOXB3 condensates. Single-cell CUT&Tag (H3K27ac); condensate/phase separation analysis; peptide disruption assay; patient-derived xenograft models; chromatin structure analysis Nature Cell Biology High 41028837

Source papers

Stage 0 corpus · 43 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1997 Overexpression of HOXB3 in hematopoietic cells causes defective lymphoid development and progressive myeloproliferation. Immunity 155 9052833
1997 Segmental regulation of Hoxb-3 by kreisler. Nature 127 9144291
2009 Epigenetic silencing of the RASSF1A tumor suppressor gene through HOXB3-mediated induction of DNMT3B expression. Molecular cell 109 19854132
1999 Conserved and distinct roles of kreisler in regulation of the paralogous Hoxa3 and Hoxb3 genes. Development (Cambridge, England) 103 9895323
2003 Reduced proliferative capacity of hematopoietic stem cells deficient in Hoxb3 and Hoxb4. Molecular and cellular biology 94 12748289
2012 miR-7 and miR-218 epigenetically control tumor suppressor genes RASSF1A and Claudin-6 by targeting HoxB3 in breast cancer. Biochemical and biophysical research communications 74 22705304
1998 Cellular proliferation and transformation induced by HOXB4 and HOXB3 proteins involves cooperation with PBX1. Oncogene 72 9692548
2019 LncRNA-UCA1 modulates progression of colon cancer through regulating the miR-28-5p/HOXB3 axis. Journal of cellular biochemistry 69 30652355
2012 HoxB3 promotes prostate cancer cell progression by transactivating CDCA3. Cancer letters 66 23219899
2002 Krox20 and kreisler co-operate in the transcriptional control of segmental expression of Hoxb3 in the developing hindbrain. The EMBO journal 57 11823429
2017 miR-375 inhibits cancer stem cell phenotype and tamoxifen resistance by degrading HOXB3 in human ER-positive breast cancer. Oncology reports 56 28075453
2018 A novel miR-375-HOXB3-CDCA3/DNMT3B regulatory circuitry contributes to leukemogenesis in acute myeloid leukemia. BMC cancer 54 29439669
2016 miR-10b Inhibits Apoptosis and Promotes Proliferation and Invasion of Endometrial Cancer Cells via Targeting HOXB3. Cancer biotherapy & radiopharmaceuticals 40 27447302
2015 The role of HOXB2 and HOXB3 in acute myeloid leukemia. Biochemical and biophysical research communications 32 26482852
2007 Hoxa9/hoxb3/hoxb4 compound null mice display severe hematopoietic defects. Experimental hematology 32 17761289
2001 Regulatory analysis of the mouse Hoxb3 gene: multiple elements work in concert to direct temporal and spatial patterns of expression. Developmental biology 31 11254356
2019 Knockdown of lncRNA-UCA1 inhibits the proliferation and migration of melanoma cells through modulating the miR-28-5p/HOXB3 axis. Experimental and therapeutic medicine 26 30988802
2016 Deregulation of MicroRNA-375 inhibits cancer proliferation migration and chemosensitivity in pancreatic cancer through the association of HOXB3. American journal of translational research 25 27186281
2005 Hoxb3 vagal neural crest-specific enhancer element for controlling enteric nervous system development. Developmental dynamics : an official publication of the American Association of Anatomists 20 15768390
2000 Regulation of Hoxb3 expression in the hindbrain and pharyngeal arches by rae28, a member of the mammalian Polycomb group of genes. Mechanisms of development 20 11044623
2002 Auto/cross-regulation of Hoxb3 expression in posterior hindbrain and spinal cord. Developmental biology 19 12482716
2011 Hoxb3 negatively regulates Hoxb1 expression in mouse hindbrain patterning. Developmental biology 17 21320481
2007 Hoxb3 deficiency impairs B lymphopoiesis in mouse bone marrow. Experimental hematology 17 17309827
1998 Regulatory interactions between the human HOXB1, HOXB2, and HOXB3 proteins and the upstream sequence of the Otx2 gene in embryonal carcinoma cells. The Journal of biological chemistry 16 9556594
2024 STUB1 suppresses paclitaxel resistance in ovarian cancer through mediating HOXB3 ubiquitination to inhibit PARK7 expression. Communications biology 12 39501077
2020 CSF levels of HoxB3 and YKL-40 may predict conversion from clinically isolated syndrome to relapsing remitting multiple sclerosis. Multiple sclerosis and related disorders 12 33352356
2022 Extracellular Vesicle-Encapsulated MicroRNA-375 from Bone Marrow-Derived Mesenchymal Stem Cells Inhibits Hepatocellular Carcinoma Progression through Regulating HOXB3-Mediated Wnt/β-Catenin Pathway. Analytical cellular pathology (Amsterdam) 11 35127344
2020 miR-7 Reduces High Glucose Induced-damage Via HoxB3 and PI3K/AKT/mTOR Signaling Pathways in Retinal Pigment Epithelial Cells. Current molecular medicine 11 31702491
2023 HOXB3 promotes trophoblast cell proliferation, invasion, and migration to alleviate preeclampsia via mediating the Notch/Wnt/β-catenin pathway. European journal of pharmacology 10 37652291
2020 Loss of HOXB3 correlates with the development of hormone receptor negative breast cancer. PeerJ 10 33240685
2007 Expression of homeotic genes Hoxa3, Hoxb3, Hoxd3 and Hoxc4 is decreased in the lungs but not in the hearts of adriamycin-exposed mice. Pediatric surgery international 10 17211587
2023 HOXB3 drives WNT-activation associated progression in castration-resistant prostate cancer. Cell death & disease 8 36973255
2020 Levels of serum Hoxb3 and sFlt-1 in pre-eclamptic patients and their effects on pregnancy outcomes. The journal of obstetrics and gynaecology research 8 32748508
2021 Long non-coding RNA LINC00514 promotes the proliferation and invasion through the miR-708-5p/HOXB3 axis in cervical squamous cell carcinoma. Environmental toxicology 7 34652879
2021 Hoxb3 Regulates Jag1 Expression in Pharyngeal Epithelium and Affects Interaction With Neural Crest Cells. Frontiers in physiology 6 33505317
1994 The 5'-sequence of the murine Hox-b3 (Hox-2.7) gene and its intron contain multiple transcription-regulatory elements. The International journal of biochemistry 6 7890121
2010 Multiple coding and non-coding RNAs in the Hoxb3 locus and their spatial expression patterns during mouse embryogenesis. Biochemical and biophysical research communications 5 20515659
2024 SIRT5 suppresses the trophoblast cell proliferation, invasion, and migration to promote preeclampsia via desuccinylating HOXB3. Journal of assisted reproduction and genetics 4 39145876
2021 Upregulation of lncRNA147410.3 in the Brain of Mice With Chronic Toxoplasma Infection Promoted Microglia Apoptosis by Regulating Hoxb3. Frontiers in cellular neuroscience 4 34084127
2021 Investigation of HoxB3 and Growth Factors Expression in Placentas of Various Gestational Ages. Journal of developmental biology 4 35076557
2025 Defining heterogeneity in core regulatory circuitry reveals HOXB3 condensation as a potential target in glioblastoma. Nature cell biology 3 41028837
2014 The absence of mutations in homeobox candidate genes HOXA3, HOXB3, HOXD3 and PITX2 in familial and sporadic thyroid hemiagenesis. Journal of pediatric endocrinology & metabolism : JPEM 3 24127533
2022 Immunohistochemical Evaluation of BARX1, DLX4, FOXE1, HOXB3, and MSX2 in Nonsyndromic Cleft Affected Tissue. Acta medica Lituanica 2 37733420

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