Affinage

HAP1

Huntingtin-associated protein 1 · UniProt P54257

Round 2 corrected
Length
671 aa
Mass
75.5 kDa
Annotated
2026-04-28
130 papers in source corpus 18 papers cited in narrative 18 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

Insufficient on-target evidence to synthesize a narrative — discovery timeline does not match the canonical HAP1 protein.

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
No controlled-vocabulary terms were assigned to this entry.

Evidence

Reading pass · 18 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 HAP1 (huntingtin-associated protein 1) interacts with the p150Glued subunit of dynactin and with the pericentriolar protein PCM-1. The interaction with p150Glued was confirmed by in vitro GST pulldown and co-immunoprecipitation from brain extracts, and both proteins co-localize in NGF-treated PC12 cells, suggesting HAP1 functions as an adaptor linking cytoskeletal, vesicular, and motor proteins. GST pulldown, co-immunoprecipitation from brain extracts, double-label immunofluorescence confocal microscopy Human molecular genetics High 9361024
1997 HAP1 interacts with a Trio-like polypeptide (Duo) containing a Rac1 guanine nucleotide exchange factor (GEF) domain, a pleckstrin homology domain, and spectrin-like repeats. The interaction was identified by yeast two-hybrid screen and confirmed by in vitro protein-binding assay, suggesting HAP1 may link huntingtin to a Ras-related signaling pathway. Yeast two-hybrid screen, in vitro protein-binding assay Human molecular genetics Medium 9285789
1996 HAP1 is enriched in neurons and localizes to subcellular fractions containing synaptic vesicles. HAP1 and neuronal nitric oxide synthase (nNOS) co-localize in discrete neuronal populations and share similar brain regional distributions, with both enriched in the pedunculopontine nuclei, accessory olfactory bulb, and supraoptic nucleus. In situ hybridization, subcellular fractionation, immunocytochemistry Proceedings of the National Academy of Sciences of the United States of America Medium 8643490
1998 Human HAP1 (hHAP) is specifically expressed in brain tissues, encodes a 75-kDa protein, and binds to huntingtin in a polyglutamine repeat length-dependent manner (longer repeats bind more tightly). The interaction was confirmed by in vitro binding, co-immunoprecipitation, and coexpression studies. cDNA cloning, in vitro binding assay, co-immunoprecipitation, coexpression in mammalian cells The Journal of biological chemistry High 9668110
1998 HAP1 isoforms (HAP1-A and HAP1-B) associate with a unique cytoplasmic structure in neurons resembling the stigmoid body/nematosome. HAP1-A, but not HAP1-B, is essential for forming this inclusion structure in transfected cell lines. Both isoforms self-associate, and the ratio of HAP1-A to HAP1-B regulates inclusion formation. Immunocytochemistry, electron microscopy, transfection of cell lines, yeast two-hybrid for self-association Journal of neurochemistry Medium 9798945
2002 Targeted disruption of HAP1 (Hap1-/-) in mice causes postnatal lethality due to severely depressed feeding behavior, malnutrition, and dehydration. 70% die by P2 and 100% by P9. HAP1 is particularly enriched in the hypothalamus, implicating it as essential for regulating postnatal feeding behavior. Homologous recombination knockout, behavioral observation, serum leptin measurement, brain weight analysis Human molecular genetics High 11971876
2003 HAP1 interacts with NeuroD (a bHLH transcription factor) and serves as a scaffold facilitating NeuroD activation by MLK2 kinase. HAP1 and huntingtin facilitate MLK2-mediated phosphorylation and activation of NeuroD, identifying a neuron-specific transcriptional regulatory complex. Yeast two-hybrid, co-immunoprecipitation, transcriptional reporter assays, kinase assay Proceedings of the National Academy of Sciences of the United States of America Medium 12881483
2004 HAP1 null mutants rescued from early lethality (by litter size reduction) survive to adulthood with growth retardation but no overt brain or behavioral abnormalities, demonstrating HAP1 is required specifically for early postnatal feeding. Conditional gene repair restoring neuronal HAP1 expression before birth rescues early lethality. No synergism between Hap1 and huntingtin mutations was observed during development. Conditional gene rescue (neuronal-specific), Hap1 null mouse characterization, litter size manipulation Human molecular genetics High 15496430
2010 HAP1 acts as an adaptor linking GABA-A receptors (GABAARs) to the kinesin motor KIF5, mediating anterograde delivery of GABAARs to synapses. Disrupting the HAP1-KIF5 complex reduces synaptic GABAAR number and decreases inhibitory postsynaptic current amplitude. Mutant huntingtin (polyQ-expanded) impairs GABAAR transport via disruption of this complex. Co-immunoprecipitation, RNAi knockdown, electrophysiology (mIPSC recording), live imaging of receptor trafficking Neuron High 20152113
2011 HAP1 interacts with PCM1 (pericentriolar material 1 protein) and huntingtin (HTT) to regulate ciliogenesis. Loss of Htt in mouse cells impairs retrograde trafficking of PCM1 and reduces primary cilia formation. In HD mice, pathogenic polyQ-expanded HTT causes centrosomal accumulation of PCM1 and abnormally long primary cilia in striatal cells, altering CSF flow. Co-immunoprecipitation, immunofluorescence, conditional knockout (ependymal cell-specific Htt deletion), cilia length measurement in HD mouse models and patient tissue The Journal of clinical investigation High 21985783
2011 proBDNF forms a trimeric complex with HAP1 and sortilin that regulates proBDNF intracellular trafficking and stabilization. The HAP1-proBDNF interaction was confirmed by FRET and co-immunoprecipitation. GST pulldown mapping defined the HAP1 binding region (aa 371–445) for proBDNF. FRAP experiments in HAP1-/- neurons showed defective proBDNF vesicle movement, partially rescued by HAP1 re-expression. The complex also facilitates furin cleavage to release mature BDNF. Co-immunoprecipitation, FRET, GST pulldown domain mapping, FRAP in HAP1-/- neurons, Western blot The Journal of biological chemistry High 21357693
2011 Mecp2 deficiency reduces Htt and Hap1 levels in mouse brain, causing defective BDNF axonal transport in the cortico-striatal pathway. Velocity of BDNF-containing vesicles is reduced in Mecp2-deficient axons and rescued by Mecp2 re-expression. APP transport (also Htt/Hap1-dependent) is similarly impaired, placing HAP1 in the Mecp2-Htt-Hap1 axis controlling axonal transport. Live imaging of BDNF vesicle transport, in vitro neuronal culture, viral rescue, mouse behavioral and lifespan analysis Neurobiology of disease Medium 22127389
2014 Huntingtin (HTT) and HAP1 copurify and colocalize with autophagosomes in neurons. Using RNAi in primary neurons from GFP-LC3 mice and live-cell imaging, HTT and HAP1 were shown to control autophagosome dynamics by regulating dynein and kinesin motors to promote processive retrograde transport. PolyQ-HTT expression disrupts autophagosomal transport and leads to inefficient cargo degradation, without affecting autophagosome formation or cargo loading. Autophagosome purification/co-purification, RNAi knockdown, live-cell imaging in primary neurons (GFP-LC3 mice), mitochondrial fragment degradation assay The Journal of neuroscience High 24453320
2006 HAP1 interacts with androgen receptor (AR) through the AR ligand-binding domain in a polyglutamine length-dependent manner. HAP1 sequesters polyQ-expanded AR into inclusions (stigmoid bodies) and suppresses SBMA-mutant AR-induced apoptosis when cotransfected in HEp-2 cells. Dihydrotestosterone reduces HAP1-AR association more strongly for normal than expanded polyQ-AR. Co-transfection, co-immunoprecipitation, immunofluorescence, apoptosis assay Human molecular genetics Medium 16782802
2016 HAP1 is required for GABAAR recycling to the neuronal surface. In hippocampal neurons subjected to oxygen/glucose deprivation (OGD), calpain cleaves HAP1, reducing GABAAR recycling. Overexpression of HAP1A or HAP1B isoforms restored GABAAR surface expression and reduced OGD-induced neuronal death, demonstrating HAP1's role in receptor recycling and neuroprotection during ischemia. Transfection with myc-tagged GABAAR β3, surface biotinylation, co-immunoprecipitation, calpain cleavage assay, cell death assay in OGD model Molecular neurobiology Medium 26732589
2017 HAP1 regulates neuronal endocytosis and interacts with clathrin light chain B (validated by Co-IP and GST pulldown) and Sec23A (ER-to-Golgi vesicle coat; validated by endogenous Co-IP in rat brain). HAP1 co-localizes with clathrin light chain B. HAP1 knockout adrenal chromaffin cells show dramatically reduced vesicle retrieval and endocytosis, and HAP1-/- cortical neurons show impaired transferrin endocytosis. Affinity chromatography/mass spectrometry proteomics, Co-IP, GST pulldown, immunofluorescence co-localization, endocytosis assay in HAP1-/- neurons Cellular signalling High 28259758
2019 HAP1 forms a ternary complex with huntingtin and the IP3 receptor (inositol 1,4,5-trisphosphate receptor, an intracellular Ca2+ channel) on the ER membrane. Loss of HAP1 prevents this ternary complex formation, blocks ER Ca2+ release upon IP3 stimulation, inhibits external Ca2+ entry, and reduces activation of the Ca2+-dependent calpain-1-Bid-caspase-3/12 apoptotic pathway, causing l-asparaginase resistance in ALL cells. Genome-wide RNAi screen, Co-immunoprecipitation (ternary complex), Ca2+ imaging, calpain/caspase activity assays, HAP1 knockdown rescue experiments Blood High 30819925
2020 AAV-mediated depletion of Hap1 in adult HD knock-in mouse brains causes selective neuronal loss in the striatum, but only in the presence of mutant HTT. Rhes (a striatum-enriched GTPase) binds more N-terminal HTT when Hap1 is absent, and more soluble sumoylated N-terminal HTT accumulates in the striatum, suggesting Hap1 normally sequesters or protects against toxic HTT fragments and that Rhes-Hap1 cooperate to determine selective striatal vulnerability. AAV-mediated gene silencing in vivo, histological analysis of neuronal loss, Co-immunoprecipitation, Western blot for sumoylated HTT Proceedings of the National Academy of Sciences of the United States of America Medium 32747555

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2016 ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure. Cell 1233 26777405
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2011 Hydroxylation of 5-methylcytosine by TET1 promotes active DNA demethylation in the adult brain. Cell 1055 21496894
2012 The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. Molecular cell 973 22681889
2005 Nucleolar proteome dynamics. Nature 934 15635413
2004 Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nature biotechnology 916 15592455
1992 Redox activation of Fos-Jun DNA binding activity is mediated by a DNA repair enzyme. The EMBO journal 849 1380454
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
1997 AP-1 transcriptional activity is regulated by a direct association between thioredoxin and Ref-1. Proceedings of the National Academy of Sciences of the United States of America 698 9108029
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2000 DNA-bound structures and mutants reveal abasic DNA binding by APE1 and DNA repair coordination [corrected]. Nature 640 10667800
2010 An atlas of combinatorial transcriptional regulation in mouse and man. Cell 573 20211142
1991 Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes. Proceedings of the National Academy of Sciences of the United States of America 479 1722334
2003 Tumor suppressor NM23-H1 is a granzyme A-activated DNase during CTL-mediated apoptosis, and the nucleosome assembly protein SET is its inhibitor. Cell 439 12628186
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2005 Genetic polymorphisms in the base excision repair pathway and cancer risk: a HuGE review. American journal of epidemiology 431 16221808
1997 Identification of redox/repair protein Ref-1 as a potent activator of p53. Genes & development 423 9119221
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2001 XRCC1 coordinates the initial and late stages of DNA abasic site repair through protein-protein interactions. The EMBO journal 392 11707423
2017 Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions. Nature biotechnology 378 28319085
1998 Activation of apurinic/apyrimidinic endonuclease in human cells by reactive oxygen species and its correlation with their adaptive response to genotoxicity of free radicals. Proceedings of the National Academy of Sciences of the United States of America 359 9560228
1999 Thioredoxin-dependent redox regulation of p53-mediated p21 activation. The Journal of biological chemistry 356 10585464
1989 Identification and characterization of HAP4: a third component of the CCAAT-bound HAP2/HAP3 heteromer. Genes & development 349 2676721
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2005 Polymorphisms of DNA repair genes and risk of non-small cell lung cancer. Carcinogenesis 333 16195237
2005 HIF-1alpha, STAT3, CBP/p300 and Ref-1/APE are components of a transcriptional complex that regulates Src-dependent hypoxia-induced expression of VEGF in pancreatic and prostate carcinomas. Oncogene 329 15735682
2014 The regulation of autophagosome dynamics by huntingtin and HAP1 is disrupted by expression of mutant huntingtin, leading to defective cargo degradation. The Journal of neuroscience : the official journal of the Society for Neuroscience 300 24453320
1997 The crystal structure of the human DNA repair endonuclease HAP1 suggests the recognition of extra-helical deoxyribose at DNA abasic sites. The EMBO journal 282 9351835
1989 Functional dissection and sequence of yeast HAP1 activator. Cell 279 2643482
1997 Huntingtin-associated protein 1 (HAP1) interacts with the p150Glued subunit of dynactin. Human molecular genetics 275 9361024
2008 The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes. Genes & development 252 18367645
2006 Arabidopsis HAP2 (GCS1) is a sperm-specific gene required for pollen tube guidance and fertilization. Development (Cambridge, England) 232 17079265
2010 Delivery of GABAARs to synapses is mediated by HAP1-KIF5 and disrupted by mutant huntingtin. Neuron 211 20152113
1987 Yeast HAP2 and HAP3 activators both bind to the CYC1 upstream activation site, UAS2, in an interdependent manner. Cell 211 2826015
1994 A role for the human DNA repair enzyme HAP1 in cellular protection against DNA damaging agents and hypoxic stress. Nucleic acids research 200 7800476
1987 Yeast HAP1 activator competes with the factor RC2 for binding to the upstream activation site UAS1 of the CYC1 gene. Cell 196 3030567
1998 Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites. Genes & development 180 9765213
1999 Molecular mechanism of heme signaling in yeast: the transcriptional activator Hap1 serves as the key mediator. Cellular and molecular life sciences : CMLS 166 11212295
1990 The HAP2 subunit of yeast CCAAT transcriptional activator contains adjacent domains for subunit association and DNA recognition: model for the HAP2/3/4 complex. Genes & development 154 2123465
2006 Global structural changes in hepatitis B virus capsids induced by the assembly effector HAP1. Journal of virology 150 16943288
2017 The Ancient Gamete Fusogen HAP2 Is a Eukaryotic Class II Fusion Protein. Cell 136 28235200
1993 Mutations in yeast HAP2/HAP3 define a hybrid CCAAT box binding domain. The EMBO journal 136 8223474
2011 Ciliogenesis is regulated by a huntingtin-HAP1-PCM1 pathway and is altered in Huntington disease. The Journal of clinical investigation 130 21985783
1987 Sequence and nuclear localization of the Saccharomyces cerevisiae HAP2 protein, a transcriptional activator. Molecular and cellular biology 123 3547076
1985 Cloning and molecular analysis of the HAP2 locus: a global regulator of respiratory genes in Saccharomyces cerevisiae. Molecular and cellular biology 122 3915775
1996 Huntingtin-associated protein (HAP1): discrete neuronal localizations in the brain resemble those of neuronal nitric oxide synthase. Proceedings of the National Academy of Sciences of the United States of America 121 8643490
1995 Identification of critical active-site residues in the multifunctional human DNA repair enzyme HAP1. Nature structural biology 120 7664124
1999 A 'natural' mutation in Saccharomyces cerevisiae strains derived from S288c affects the complex regulatory gene HAP1 (CYP1). Current genetics 119 10541856
1988 Mutational analysis of upstream activation sequence 2 of the CYC1 gene of Saccharomyces cerevisiae: a HAP2-HAP3-responsive site. Molecular and cellular biology 119 2832731
1997 Huntingtin-associated protein 1 (HAP1) binds to a Trio-like polypeptide, with a rac1 guanine nucleotide exchange factor domain. Human molecular genetics 116 9285789
1988 Yeast HAP2 and HAP3: transcriptional activators in a heteromeric complex. Science (New York, N.Y.) 115 2832951
1990 The B subunit of a rat heteromeric CCAAT-binding transcription factor shows a striking sequence identity with the yeast Hap2 transcription factor. Proceedings of the National Academy of Sciences of the United States of America 109 2196566
2007 Heme levels switch the function of Hap1 of Saccharomyces cerevisiae between transcriptional activator and transcriptional repressor. Molecular and cellular biology 108 17785431
1992 HAP1 and ROX1 form a regulatory pathway in the repression of HEM13 transcription in Saccharomyces cerevisiae. Molecular and cellular biology 105 1588959
2002 A microarray-assisted screen for potential Hap1 and Rox1 target genes in Saccharomyces cerevisiae. Yeast (Chichester, England) 103 12112237
2009 Plasmodium berghei HAP2 induces strong malaria transmission-blocking immunity in vivo and in vitro. Vaccine 92 19596419
1994 The yeast activator HAP1--a GAL4 family member--binds DNA in a directly repeated orientation. Genes & development 91 7958882
1988 Differential regulation of the two genes encoding Saccharomyces cerevisiae cytochrome c oxidase subunit V by heme and the HAP2 and REO1 genes. Molecular and cellular biology 90 2847035
1999 Structure of a HAP1-DNA complex reveals dramatically asymmetric DNA binding by a homodimeric protein. Nature structural biology 85 9886294
2004 Transcriptional dysregulation in striatal projection- and interneurons in a mouse model of Huntington's disease: neuronal selectivity and potential neuroprotective role of HAP1. Human molecular genetics 84 15548548
2017 Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens. The Journal of cell biology 81 28137780
2017 Structure-Function Studies Link Class II Viral Fusogens with the Ancestral Gamete Fusion Protein HAP2. Current biology : CB 76 28238660
2002 Targeted disruption of Huntingtin-associated protein-1 (Hap1) results in postnatal death due to depressed feeding behavior. Human molecular genetics 73 11971876
1991 Regulation of the yeast CYT1 gene encoding cytochrome c1 by HAP1 and HAP2/3/4. Molecular and cellular biology 73 1656218
2002 The yeast transcriptome in aerobic and hypoxic conditions: effects of hap1, rox1, rox3 and srb10 deletions. Molecular microbiology 71 11929514
2017 Targeting the Conserved Fusion Loop of HAP2 Inhibits the Transmission of Plasmodium berghei and falciparum. Cell reports 69 29212032
2003 A mechanism of oxygen sensing in yeast. Multiple oxygen-responsive steps in the heme biosynthetic pathway affect Hap1 activity. The Journal of biological chemistry 67 14512429
1991 The Schizosaccharomyces pombe homolog of Saccharomyces cerevisiae HAP2 reveals selective and stringent conservation of the small essential core protein domain. Molecular and cellular biology 67 1899284
2000 Ultraviolet B radiation-induced skin cancer in mice defective in the Xpc, Trp53, and Apex (HAP1) genes: genotype-specific effects on cancer predisposition and pathology of tumors. Cancer research 62 10749126
1998 A human HAP1 homologue. Cloning, expression, and interaction with huntingtin. The Journal of biological chemistry 62 9668110
1994 Subunit interaction in the CCAAT-binding heteromeric complex is mediated by a very short alpha-helix in HAP2. Proceedings of the National Academy of Sciences of the United States of America 62 8159696
2005 HAP1 and intracellular trafficking. Trends in pharmacological sciences 61 15629196
1993 Evidence for an interaction between the CYP1(HAP1) activator and a cellular factor during heme-dependent transcriptional regulation in the yeast Saccharomyces cerevisiae. The EMBO journal 61 8458333
1991 Complex transcriptional regulation of the Saccharomyces cerevisiae CYB2 gene encoding cytochrome b2: CYP1(HAP1) activator binds to the CYB2 upstream activation site UAS1-B2. Molecular and cellular biology 61 2046677
2011 Precursor of brain-derived neurotrophic factor (proBDNF) forms a complex with Huntingtin-associated protein-1 (HAP1) and sortilin that modulates proBDNF trafficking, degradation, and processing. The Journal of biological chemistry 60 21357693
2003 Stimulation of NeuroD activity by huntingtin and huntingtin-associated proteins HAP1 and MLK2. Proceedings of the National Academy of Sciences of the United States of America 60 12881483
2011 Modification of Mecp2 dosage alters axonal transport through the Huntingtin/Hap1 pathway. Neurobiology of disease 55 22127389
1992 Structure of the human DNA repair gene HAP1 and its localisation to chromosome 14q 11.2-12. Nucleic acids research 55 1383925
2000 Substitution of Asp-210 in HAP1 (APE/Ref-1) eliminates endonuclease activity but stabilises substrate binding. Nucleic acids research 54 10871340
2014 Function of the male-gamete-specific fusion protein HAP2 in a seven-sexed ciliate. Current biology : CB 52 25155508
1997 Stationary-phase regulation of the Saccharomyces cerevisiae SOD2 gene is dependent on additive effects of HAP2/3/4/5- and STRE-binding elements. Molecular microbiology 51 9044264
2018 Evolutionary diversification of the HAP2 membrane insertion motifs to drive gamete fusion across eukaryotes. PLoS biology 50 30102690
2005 Polymerase chain reaction assay specific for pathogenic Leptospira based on the gene hap1 encoding the hemolysis-associated protein-1. FEMS microbiology letters 50 15686847
1998 Association of HAP1 isoforms with a unique cytoplasmic structure. Journal of neurochemistry 48 9798945
1993 Antibody-promoted dimerization bypasses the regulation of DNA binding by the heme domain of the yeast transcriptional activator HAP1. Proceedings of the National Academy of Sciences of the United States of America 48 8464899
2006 Huntingtin-associated protein 1 (HAP1) interacts with androgen receptor (AR) and suppresses SBMA-mutant-AR-induced apoptosis. Human molecular genetics 46 16782802
1996 Mutations in target DNA elements of yeast HAP1 modulate its transcriptional activity without affecting DNA binding. Nucleic acids research 45 8628677
2001 The Hsp70-Ydj1 molecular chaperone represses the activity of the heme activator protein Hap1 in the absence of heme. Molecular and cellular biology 44 11689685
1998 The expression of Huntingtin-associated protein (HAP1) mRNA in developing, adult and ageing rat CNS: implications for Huntington's disease neuropathology. The European journal of neuroscience 44 9751154
1996 Asparagine 212 is essential for abasic site recognition by the human DNA repair endonuclease HAP1. Nucleic acids research 44 8932375
1992 Regulation of yeast COX6 by the general transcription factor ABF1 and separate HAP2- and heme-responsive elements. Molecular and cellular biology 42 1314953
1991 CYP1 (HAP1) is a determinant effector of alternative expression of heme-dependent transcribed genes in yeast [corrected]. Molecular & general genetics : MGG 42 1715975
2009 Evolutionary history of the HAP2/GCS1 gene and sexual reproduction in metazoans. PloS one 41 19888453
2004 Huntingtin-associated protein 1 (Hap1) mutant mice bypassing the early postnatal lethality are neuroanatomically normal and fertile but display growth retardation. Human molecular genetics 41 15496430
2000 Functional analysis of heme regulatory elements of the transcriptional activator Hap1. Biochemical and biophysical research communications 41 10873649
2017 The Babesia bovis hap2 gene is not required for blood stage replication, but expressed upon in vitro sexual stage induction. PLoS neglected tropical diseases 40 28985216
2005 Mapping the laminin-binding and adhesive domain of the cell surface-associated Hlp/LBP protein from Mycobacterium leprae. Microbes and infection 40 15919224
2001 The HAP1 protein stimulates the turnover of human mismatch-specific thymine-DNA-glycosylase to process 3,N(4)-ethenocytosine residues. Mutation research 40 11506820
1996 The C6 zinc cluster dictates asymmetric binding by HAP1. The EMBO journal 39 8887558
1994 The HAP2,3,4 transcriptional activator is required for derepression of the yeast citrate synthase gene, CIT1. Molecular microbiology 39 7984086
1994 Evidence that TUP1/SSN6 has a positive effect on the activity of the yeast activator HAP1. Genetics 39 8005436
1992 Positive regulation of the LPD1 gene of Saccharomyces cerevisiae by the HAP2/HAP3/HAP4 activation system. Molecular & general genetics : MGG 38 1310523
2019 HAP1 loss confers l-asparaginase resistance in ALL by downregulating the calpain-1-Bid-caspase-3/12 pathway. Blood 37 30819925
2010 Age at onset in Huntington's disease: replication study on the associations of ADORA2A, HAP1 and OGG1. Neurogenetics 37 20512606
1999 A new class of repression modules is critical for heme regulation of the yeast transcriptional activator Hap1. Molecular and cellular biology 36 10330173
2005 The heme activator protein Hap1 represses transcription by a heme-independent mechanism in Saccharomyces cerevisiae. Genetics 34 15654089
1998 Differential cleavage of oligonucleotides containing the benzene-derived adduct, 1,N6-benzetheno-dA, by the major human AP endonuclease HAP1 and Escherichia coli exonuclease III and endonuclease IV. Carcinogenesis 34 9744526
1997 The structure and functions of the HAP1/Ref-1 protein. Oncology research 34 9406232
2020 Loss of Hap1 selectively promotes striatal degeneration in Huntington disease mice. Proceedings of the National Academy of Sciences of the United States of America 33 32747555
2020 Efficient and crucial quality control of HAP1 cell ploidy status. Biology open 33 33184093
2018 Fusion surface structure, function, and dynamics of gamete fusogen HAP2. eLife 32 30281023
2009 The Chlamydomonas chloroplast HLP protein is required for nucleoid organization and genome maintenance. Molecular plant 32 19995727
1999 Overexpression of the human HAP1 protein sensitizes cells to the lethal effect of bioreductive drugs. Carcinogenesis 32 10190555
1995 The respiratory system of Kluyveromyces lactis escapes from HAP2 control. Gene 32 7828916
2016 Downregulation of GABAA Receptor Recycling Mediated by HAP1 Contributes to Neuronal Death in In Vitro Brain Ischemia. Molecular neurobiology 31 26732589
2022 Discovery of archaeal fusexins homologous to eukaryotic HAP2/GCS1 gamete fusion proteins. Nature communications 30 35794124
2022 DMP8 and 9 regulate HAP2/GCS1 trafficking for the timely acquisition of sperm fusion competence. Proceedings of the National Academy of Sciences of the United States of America 30 36322734
2008 The C-terminal domain of HU-related histone-like protein Hlp from Mycobacterium smegmatis mediates DNA end-joining. Biochemistry 30 18656956
2007 Regulation of intracellular HAP1 trafficking. Journal of neuroscience research 30 17474105
2003 Structural environment dictates the biological significance of heme-responsive motifs and the role of Hsp90 in the activation of the heme activator protein Hap1. Molecular and cellular biology 30 12897155
1999 The yeast heme-responsive transcriptional activator Hap1 is a preexisting dimer in the absence of heme. The Journal of biological chemistry 30 10428861
2017 Huntingtin-associated protein-1 (HAP1) regulates endocytosis and interacts with multiple trafficking-related proteins. Cellular signalling 29 28259758
1998 Assembly characteristics of flagellar cap protein HAP2 of Salmonella: decamer and pentamer in the pH-sensitive equilibrium. Journal of molecular biology 29 9545379
2002 The molecular chaperone Hsp90 mediates heme activation of the yeast transcriptional activator Hap1. The Journal of biological chemistry 28 11751848
1990 Bacterial 'histone-like protein I' (HLP-I) is an outer membrane constituent? FEBS letters 28 2318304