Affinage

GPX7

Glutathione peroxidase 7 · UniProt Q96SL4

Round 2 corrected
Length
187 aa
Mass
21.0 kDa
Annotated
2026-04-28
57 papers in source corpus 18 papers cited in narrative 18 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

GPX7 is an ER-resident, non-selenocysteine glutathione peroxidase that functions primarily as a redox sensor and disulfide relay rather than a classical peroxide scavenger. Its peroxidatic Cys57 is oxidized to sulfenic acid by H₂O₂—including Ero1α-generated H₂O₂—and resolved by Cys86 to form an intramolecular disulfide; both the sulfenic acid and disulfide forms oxidize PDI (and P5), coupling peroxide consumption to oxidative protein folding via an Ero1α/GPX7/PDI triad that generates two disulfide bonds per O₂ consumed (PMID:23919619, PMID:32719007). Beyond PDI, the activated disulfide form of GPX7 engages diverse client proteins through intermolecular disulfide bonds—GRP78 (enhancing chaperone activity) (PMID:23123197), CPEB2 (suppressing HIF-1α translation) (PMID:26446990), OGA (modulating O-GlcNAcylation to protect motor neurons) (PMID:31747588), and ZAP70 (dampening TCR signaling to restrain T cell hyperactivation) (PMID:33460768)—thereby serving as a versatile redox signal transmitter linking ER oxidative status to protein homeostasis, metabolic regulation, and immune tolerance. GPX7 deficiency in mice causes obesity through ROS-dependent PKA-regulatory-subunit dimerization and enhanced adipogenesis (PMID:23828861), ALS-like motor neuron degeneration (PMID:31747588), and T cell-driven autoimmune susceptibility (PMID:33460768), while its epigenetic silencing by promoter hypermethylation is frequent in esophageal adenocarcinoma where it acts as a tumor suppressor (PMID:23580780).

Mechanistic history

Synthesis pass · year-by-year structured walk · 10 steps
  1. 2004 High

    Establishing that GPX7 is a non-selenocysteine GPx family member with minimal classical peroxidase activity answered whether the protein functions as a conventional glutathione peroxidase—it does not, yet still protects cells from oxidative stress.

    Evidence In vitro GPx activity assay, siRNA knockdown, and overexpression in breast cancer cells exposed to EPA-derived ROS

    PMID:15294905

    Open questions at the time
    • Mechanism of protection without classical GPx activity was unknown
    • Subcellular localization was initially assigned as cytoplasmic
  2. 2011 High

    Demonstration that GPX7 resides in the ER and cooperates with Ero1α and PDI to drive oxidative protein refolding established its principal biochemical function as a PDI peroxidase rather than a glutathione-dependent scavenger.

    Evidence In vitro oxidative refolding reconstitution, co-immunoprecipitation with Ero1α, oxygen consumption assay

    PMID:21215271 PMID:22157330

    Open questions at the time
    • Catalytic mechanism and role of individual cysteines unresolved
    • Whether GPX7 uses GSH or PDI as its physiological reductant was unclear
  3. 2012 High

    Identifying GPX7 as a redox stress sensor that transmits oxidative signals to GRP78 via covalent disulfide intermediates (Cys86–Cys41/Cys420) shifted the paradigm from antioxidant to redox relay, explaining how it enhances chaperone activity and protein homeostasis.

    Evidence Active-site cysteine mutagenesis, disulfide trapping, chaperone activity assay, GPX7-knockout mice

    PMID:23123197

    Open questions at the time
    • Whether other ER chaperones are similarly regulated was not addressed
    • Structural basis of the GPX7–GRP78 disulfide intermediate was not resolved
  4. 2013 High

    Detailed kinetic and mechanistic characterization resolved the catalytic cycle: H₂O₂ oxidizes Cys57 to sulfenic acid, Cys86 resolves it to an intramolecular disulfide, and both forms can oxidize PDI, with the Ero1α/GPX7/PDI triad generating two disulfides per O₂.

    Evidence In vitro reconstitution with mutagenesis, sulfenic acid trapping, SPR (KD 5.2 µM for PDI), oxygen consumption stoichiometry

    PMID:23454490 PMID:23919619

    Open questions at the time
    • Crystal structure of the sulfenic acid intermediate was lacking
    • Relative contributions of one-Cys vs two-Cys mechanisms in vivo unresolved
  5. 2013 High

    GPX7 knockout mice develop obesity via ROS-dependent PKA regulatory subunit dimerization and C/EBPβ activation in preadipocytes, revealing GPX7 as a metabolic regulator beyond ER protein folding.

    Evidence GPX7-knockout mouse phenotyping, adipocyte differentiation assays, NAC rescue, human SNP association

    PMID:23828861

    Open questions at the time
    • Whether the adipogenic phenotype depends on ER stress vs cytosolic ROS was unresolved
    • Human genetic association was limited to SNP correlation
  6. 2013 High

    Epigenetic silencing of GPX7 by promoter hypermethylation in 69% of esophageal adenocarcinomas, together with growth-suppressive and senescence-inducing activity upon reconstitution, established GPX7 as a tumor suppressor in this tissue context.

    Evidence Colony formation, EdU proliferation, mouse xenograft, pyrosequencing of CpG methylation

    PMID:23580780

    Open questions at the time
    • Whether tumor-suppressive function operates through PDI oxidation, NF-κB suppression, or other pathways was unclear
    • Relevance to cancers beyond esophageal adenocarcinoma was not tested
  7. 2015 Medium

    Discovery that GPX7 forms a disulfide bond with CPEB2 to suppress HIF-1α mRNA translation under basal conditions—disrupted under high ROS—extended the redox relay model to translational control of hypoxia signaling.

    Evidence Co-IP, disulfide trapping, RNA–protein interaction assay, HIF-1α translation readout in GPX7-deficient cells

    PMID:26446990

    Open questions at the time
    • Whether GPX7–CPEB2 interaction regulates other mRNA targets was not explored
    • In vivo validation of HIF-1α translational control was limited
  8. 2019 High

    GPX7 inhibits O-GlcNAcase (OGA) by disulfide bonding, fine-tuning O-GlcNAcylation to protect spinal motor neurons; GPX7-knockout mice develop ALS-like phenotypes rescued by OGA inhibition, linking the redox relay to neurodegeneration.

    Evidence GPX7-knockout mouse ALS phenotype, disulfide trapping of GPX7–OGA complex, pharmacological OGA inhibitor rescue

    PMID:31747588

    Open questions at the time
    • Whether GPX7–OGA interaction occurs in cell types beyond motor neurons was not assessed
    • Upstream signals that activate GPX7 in aging motor neurons remain undefined
  9. 2020 High

    Refined enzymological comparison showed GPX7 has much higher H₂O₂ reactivity than GPX8, attributable to a catalytic tetrad stabilizing sulfenylated Cys57; the resolving Cys86 controls PDI oxidation activity, and GPX7 preferentially forms complexes with PDI and P5.

    Evidence In vitro H₂O₂ reactivity and PDI oxidation assays, mutagenesis, co-IP in H₂O₂-treated cells

    PMID:32719007

    Open questions at the time
    • Full structural determination of the catalytic tetrad was not provided
    • Quantitative contribution of GPX7 vs GPX8 to ER redox homeostasis in vivo remains unresolved
  10. 2021 High

    T cell-specific GPX7 knockout revealed that GPX7 restrains TCR signaling by forming a disulfide bond with ZAP70 that reduces its lipid-raft recruitment, establishing GPX7 as an immune checkpoint via redox relay.

    Evidence Proteomic identification of GPX7–ZAP70 disulfide, conditional T cell-specific KO mouse, TCR activation assays, lipid raft fractionation, EAE autoimmune model

    PMID:33460768

    Open questions at the time
    • Whether GPX7 modulates other proximal TCR kinases is unknown
    • Relevance to human autoimmune disease has not been tested

Open questions

Synthesis pass · forward-looking unresolved questions
  • A high-resolution crystal structure of GPX7 in the sulfenylated and disulfide states is lacking, and the hierarchy of client selection—how GPX7 discriminates among PDI, GRP78, CPEB2, OGA, and ZAP70—remains mechanistically unresolved.
  • No crystal structure of catalytic intermediates
  • Determinants of client selectivity unknown
  • Relative importance of GPX7 vs GPX8 in ER redox homeostasis in vivo not quantified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016209 antioxidant activity 5 GO:0016491 oxidoreductase activity 5 GO:0098772 molecular function regulator activity 4
Localization
GO:0005783 endoplasmic reticulum 4
Pathway
R-HSA-392499 Metabolism of proteins 5 R-HSA-162582 Signal Transduction 4 R-HSA-8953897 Cellular responses to stimuli 4 R-HSA-168256 Immune System 1
Partners
CPEB2ERO1AGRP78OGAP5PDIXRN2ZAP70

Evidence

Reading pass · 18 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2004 GPX7 (NPGPx) was identified as a cytoplasmic ~22 kDa protein that incorporates cysteine instead of selenocysteine at the conserved catalytic motif, exhibits little detectable glutathione peroxidase activity in vitro, and protects against oxidative stress generated from polyunsaturated fatty acid (EPA) metabolism in breast cancer cells. In vitro GPx activity assay, siRNA knockdown, ectopic overexpression, cell viability assays The Journal of biological chemistry High 15294905
2011 GPX7 and GPX8 were shown to be ER-resident PDI peroxidases: in vitro, addition of GPX7 or GPX8 together with PDI and peroxide enables efficient oxidative refolding of reduced denatured protein; both proteins interact with Ero1α in vivo, and GPX7 significantly increases oxygen consumption by Ero1α in vitro. In vitro oxidative refolding assay, co-immunoprecipitation (in vivo), oxygen consumption assay Journal of molecular biology High 21215271
2011 GPX7 exhibits H2O2-neutralizing activity independent of glutathione; reconstitution of GPX7 expression in Barrett's esophagus cells conferred resistance to H2O2-induced oxidative DNA damage, double-strand breaks, ROS-dependent JNK/p38 signaling, and apoptosis following acidic bile acid exposure. GPx enzymatic activity assay, Amplex UltraRed H2O2 assay, CM-H2DCFDA ROS assay, 8-oxoguanine and phospho-H2AX assays, siRNA knockdown, ectopic overexpression Gut High 22157330
2012 NPGPx (GPX7) acts as an oxidative stress sensor/transmitter: upon ROS accumulation, it forms an intramolecular disulfide bond between Cys57 and Cys86; this oxidized form then forms covalent disulfide intermediates with GRP78 (Cys86 of NPGPx to Cys41/Cys420 of GRP78), which subsequently promotes formation of the Cys41–Cys420 disulfide in GRP78, enhancing its chaperone activity. NPGPx-deficient cells accumulate ROS, misfolded proteins, and display impaired GRP78 chaperone activity. Mutagenesis of active-site cysteines, co-immunoprecipitation, disulfide bond trapping, chaperone activity assay, NPGPx knockout mice Molecular cell High 23123197
2011 Under non-targeting siRNA stress, NPGPx (GPX7) is selectively induced and covalently binds exoribonuclease XRN2 via disulfide bonding, facilitating XRN2-mediated degradation of accumulated non-targeting siRNA and thereby releasing cellular stress. Co-immunoprecipitation, siRNA knockdown, apoptosis assay, G1 phase cell cycle analysis Nucleic acids research Medium 21908404
2013 GPX7 utilizes Ero1α-generated H2O2 to promote oxidative protein folding. Mechanistically, H2O2 oxidizes Cys57 of GPX7 to sulfenic acid, which is resolved by Cys86 to form an intramolecular disulfide bond; both the sulfenic acid form and disulfide form of GPX7 can oxidize PDI. GPX7 preferentially interacts with the a-domain of PDI, and the Ero1α/GPX7/PDI triad generates two disulfide bonds per O2 molecule consumed. In vitro oxidative folding assay, site-directed mutagenesis (Cys57, Cys86), biochemical trapping of sulfenic acid intermediate, in vivo co-IP, oxygen consumption assay Antioxidants & redox signaling High 23919619
2013 GPX7 uses a one-Cys catalytic mechanism in which the peroxidatic Cys (CP) is rapidly oxidized by phospholipid hydroperoxide; GSH and PDI are alternative reducing substrates for re-reduction of oxidized CP. PDI-GPX7 interaction was quantified with KD = 5.2 μM by surface plasmon resonance; thioredoxin does not serve as a reducing substrate. Steady-state kinetic analysis, site-directed mutagenesis, molecular docking, surface plasmon resonance Biochimica et biophysica acta High 23454490
2013 GPX7 functions as a tumor suppressor in esophageal adenocarcinoma: reconstitution of GPX7 suppresses cell growth, impairs G1/S progression, increases cellular senescence, and elevates p73, p27, p21, p16 while decreasing phospho-RB. GPX7 is silenced by location-specific promoter DNA hypermethylation (+13 to +64 region) in 69% of OAC cases. Growth curve, colony formation, EdU proliferation assay, cell cycle analysis, senescence assay, western blot, mouse xenograft model, pyrosequencing of CpG methylation Gut High 23580780
2014 Loss of GPX7 promotes TNF-α-induced NF-κB activation in esophageal cells. GPX7 suppresses NF-κB by promoting proteasomal degradation of TNFR1 and TRAF2 upstream regulators; this suppression is independent of ROS levels and GPX7 antioxidant function. Western blot, immunofluorescence, luciferase reporter assay, siRNA knockdown, ectopic overexpression, protein degradation assays Carcinogenesis Medium 24692067
2015 NPGPx (GPX7) forms a disulfide bond with translational regulator CPEB2, which maintains CPEB2 binding to HIF-1α mRNA and suppresses HIF-1α translation under basal conditions. High oxidative stress disrupts this NPGPx–CPEB2 disulfide, releasing CPEB2 from HIF-1α mRNA and elevating HIF-1α translation. Co-immunoprecipitation, disulfide trapping, RNA-protein interaction assay, western blot for HIF-1α translation, NPGPx-deficient cell lines Nucleic acids research Medium 26446990
2013 NPGPx (GPX7) deficiency leads to obesity in mice via ROS-dependent dimerization of protein kinase A regulatory subunits and activation of C/EBPβ, promoting preadipocyte differentiation into adipocytes. NPGPx is highly expressed in preadipocytes, and NPGPx-knockout mice exhibit increased fat mass and adipocyte hypertrophy reversible by N-acetylcysteine treatment. NPGPx knockout mouse model, adipocyte differentiation assays, western blot (PKA regulatory subunit dimerization, C/EBPβ), NAC rescue experiment, SNP association in humans EMBO molecular medicine High 23828861
2017 GPX7 and GPX8 are ER-resident antioxidant enzymes; expression of GPX7 in pancreatic INS-1E β-cells attenuates FFA-mediated H2O2 accumulation in the ER, ER stress, and apoptosis without compromising insulin production or oxidative protein folding/disulfide bond formation in insulin. Ectopic expression of GPX7/GPX8, H2O2 measurement, ER stress markers (western blot), apoptosis assay, insulin content assay Free radical biology & medicine Medium 28751022
2019 NPGPx (GPX7) is activated by oxidative stress and inhibits O-GlcNAcase (OGA) through disulfide bonding, thereby fine-tuning global O-GlcNAcylation. Deficiency of NPGPx in mice causes ALS-like phenotypes (paralysis, muscle denervation, motor neuron loss) and failure to boost O-GlcNAcylation in spinal motor neurons with age; pharmacological OGA inhibition rescues spinal motor neuron loss in aged NPGPx-deficient mice. NPGPx knockout mouse model (ALS phenotype characterization), disulfide bond trapping (NPGPx–OGA interaction), proteomic identification, pharmacological rescue (OGA inhibitor), O-GlcNAcylation western blot Cell reports High 31747588
2020 Human GPX7 has much higher reactivity with H2O2 than GPX8, attributable to a catalytic tetrad at the redox-active site that stabilizes the sulfenylated Cys57 intermediate. Contrary to prior models, the resolving Cys (not the peroxidatic Cys) regulates PDI oxidation activity of GPX7. GPX7 forms complexes preferentially with PDI and P5 in H2O2-treated cells. In vitro H2O2 reactivity assay, PDI oxidation assay, site-directed mutagenesis, co-immunoprecipitation in H2O2-treated cells The Journal of biological chemistry High 32719007
2020 GPX7 knockdown in TGF-β/FFA-treated hepatic stellate cells (LX-2) elevated pro-fibrotic and pro-inflammatory gene expression and collagen synthesis; GPX7 overexpression suppressed ROS and these genes. In vivo, GPX7 knockdown accelerated NASH fibrosis in a choline-deficient high-fat diet mouse model. siRNA knockdown, ectopic overexpression, western blot/qPCR for fibrotic markers, ROS assay, in vivo mouse NASH model BMB reports Medium 32317079
2021 NPGPx (GPX7) modulates T cell homeostasis by restraining ZAP70 activity. Upon TCR stimulation, ROS activates NPGPx, which then forms a disulfide bond with ZAP70, reducing ZAP70 recruitment to the TCR/CD3 complex in membrane lipid rafts and thereby dampening TCR signaling. T cell-specific NPGPx-knockout mice display hyperproliferation, elevated cytokines, and susceptibility to EAE. Proteomic identification of NPGPx–ZAP70 disulfide complex, T cell-specific conditional KO mouse, TCR activation assays, lipid raft fractionation, EAE model Free radical biology & medicine High 33460768
2021 GPX7 deficiency reduces osteogenesis while increasing adipogenesis in BMSCs via ER stress (not via ROS alone); the osteogenic defect is rescued by ER stress antagonist but not by ROS inhibitor. Mechanistically, Gpx7 deficiency downregulates mTOR signaling during osteogenic differentiation, which is rescued by relief of ER stress. siRNA knockdown of Gpx7, osteogenic/adipogenic differentiation assays, ER stress antagonist rescue, ROS inhibitor control, mTOR pathway western blot Journal of cellular and molecular medicine Medium 34626080
2020 GPX7 (fused with PDI) mediates disulfide transfer from H2O2 to target proteins; a PDI-GPX7 fusion expressed in E. coli SHuffle cells consumed ER-equivalent H2O2 and enabled efficient disulfide bond formation, resulting in 4-fold improved yield of correctly folded IgG antibody. Recombinant fusion protein expression, antibody yield quantification in shake-flask and fermentation, functional disulfide bond formation assay Applied microbiology and biotechnology Medium 32997203

Source papers

Stage 0 corpus · 57 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Towards a proteome-scale map of the human protein-protein interaction network. Nature 2090 16189514
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2006 A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Cell 610 16713569
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2003 The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome research 285 12975309
2011 Two endoplasmic reticulum PDI peroxidases increase the efficiency of the use of peroxide during disulfide bond formation. Journal of molecular biology 216 21215271
2009 Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. American journal of human genetics 164 19913121
2005 HIV-1 viral proteins gp120 and Tat induce oxidative stress in brain endothelial cells. Brain research 158 15910762
2006 The DNA sequence and biological annotation of human chromosome 1. Nature 144 16710414
2000 Molecular mechanism of decreased glutathione content in human immunodeficiency virus type 1 Tat-transgenic mice. The Journal of biological chemistry 134 10652368
2012 Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease. Molecular cell 123 23123197
2022 The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin. Nature cell biology 122 35013556
2013 Glutathione peroxidase 7 utilizes hydrogen peroxide generated by Ero1α to promote oxidative protein folding. Antioxidants & redox signaling 100 23919619
2015 GPX4 and GPX7 over-expression in human hepatocellular carcinoma tissues. European journal of histochemistry : EJH 93 26708178
2004 Identification of a novel putative non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) essential for alleviating oxidative stress generated from polyunsaturated fatty acids in breast cancer cells. The Journal of biological chemistry 92 15294905
2020 Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains. Cell reports 79 32814053
2011 Glutathione peroxidase 7 protects against oxidative DNA damage in oesophageal cells. Gut 79 22157330
2016 NPGPx (GPx7): a novel oxidative stress sensor/transmitter with multiple roles in redox homeostasis. American journal of translational research 75 27186289
2013 Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human. EMBO molecular medicine 68 23828861
2017 ER-resident antioxidative GPx7 and GPx8 enzyme isoforms protect insulin-secreting INS-1E β-cells against lipotoxicity by improving the ER antioxidative capacity. Free radical biology & medicine 51 28751022
2015 Candidate pathway-based genetic association study of platinum and platinum-taxane related toxicity in a cohort of primary lung cancer patients. Journal of the neurological sciences 51 25586538
2016 HIV-host interactome revealed directly from infected cells. Nature microbiology 49 27375898
2010 MicroRNA-related genetic variations as predictors for risk of second primary tumor and/or recurrence in patients with early-stage head and neck cancer. Carcinogenesis 46 20819778
2013 Protein disulfide isomerase and glutathione are alternative substrates in the one Cys catalytic cycle of glutathione peroxidase 7. Biochimica et biophysica acta 44 23454490
2013 Glutathione peroxidase 7 has potential tumour suppressor functions that are silenced by location-specific methylation in oesophageal adenocarcinoma. Gut 41 23580780
2010 Glutathione pathway genetic polymorphisms and lung cancer survival after platinum-based chemotherapy. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 38 20200426
2014 Loss of glutathione peroxidase 7 promotes TNF-α-induced NF-κB activation in Barrett's carcinogenesis. Carcinogenesis 36 24692067
2020 Characterization of the endoplasmic reticulum-resident peroxidases GPx7 and GPx8 shows the higher oxidative activity of GPx7 and its linkage to oxidative protein folding. The Journal of biological chemistry 33 32719007
2021 GPX7 Facilitates BMSCs Osteoblastogenesis via ER Stress and mTOR Pathway. Journal of cellular and molecular medicine 29 34626080
2001 Human immunodeficiency virus type 1 Tat protein impairs selenoglutathione peroxidase expression and activity by a mechanism independent of cellular selenium uptake: consequences on cellular resistance to UV-A radiation. Archives of biochemistry and biophysics 29 11368344
2014 Synthetic lethal screening in the mammalian central nervous system identifies Gpx6 as a modulator of Huntington's disease. Proceedings of the National Academy of Sciences of the United States of America 28 25535386
2010 Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. Diabetes care 28 20628086
1998 Glutathione depletion associated with the HIV-1 TAT protein mediates the extracellular appearance of acidic fibroblast growth factor. Archives of biochemistry and biophysics 26 9501919
2022 GPX7 Is Targeted by miR-29b and GPX7 Knockdown Enhances Ferroptosis Induced by Erastin in Glioma. Frontiers in oncology 25 35127512
2019 NPGPx-Mediated Adaptation to Oxidative Stress Protects Motor Neurons from Degeneration in Aging by Directly Modulating O-GlcNAcase. Cell reports 25 31747588
2012 A cis-element with mixed G-quadruplex structure of NPGPx promoter is essential for nucleolin-mediated transactivation on non-targeting siRNA stress. Nucleic acids research 23 23241391
2020 GPx6 is involved in the in vitro induced capacitation and acrosome reaction in porcine sperm. Theriogenology 21 32698036
2011 Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress. Nucleic acids research 21 21908404
2018 Brazilin and Caesalpinia sappan L. extract protect epidermal keratinocytes from oxidative stress by inducing the expression of GPX7. Chinese journal of natural medicines 19 29576056
2020 GPx7 ameliorates non-alcoholic steatohepatitis by regulating oxidative stress. BMB reports 18 32317079
2015 NPGPx modulates CPEB2-controlled HIF-1α RNA translation in response to oxidative stress. Nucleic acids research 16 26446990
2016 Expression of human selenoprotein genes selh, selk, selm, sels, selv, and gpx-6 in various tumor cell lines. Doklady. Biochemistry and biophysics 12 27417721
2020 Improved production of Humira antibody in the genetically engineered Escherichia coli SHuffle, by co-expression of human PDI-GPx7 fusions. Applied microbiology and biotechnology 9 32997203
2019 GPX7 promotes the growth of human papillary thyroid carcinoma via enhancement of cell proliferation and inhibition of cell apoptosis. Translational cancer research 7 35117014
2025 Metformin's mechanism in reducing oxidative stress and promoting bone regeneration in T2DM rat BMMSCs: A focus on NRF2-GPX7 signaling pathway. Journal of dentistry 6 40306480
2024 GPX7 reduces chondrocyte inflammation and extracellular matrix degradation triggered by IL‑1β, via a mechanism mediated by ferroptosis. Molecular medicine reports 6 38757339
2025 Multi-Omics and Experimental Validation Identify GPX7 and Glutathione-Associated Oxidative Stress as Potential Biomarkers in Ischemic Stroke. Antioxidants (Basel, Switzerland) 4 40563300
2025 Co-Exposure to Different Zinc Concentrations and High-Fat Diet Modules Endoplasmic Reticulum Stress and Lipotoxicity through the MTF-1/GPx7 Axis in Yellow Catfish (Pelteobagrus fulvidraco). Journal of agricultural and food chemistry 2 40238493
2021 Redox sensor NPGPx restrains ZAP70 activity and modulates T cell homeostasis. Free radical biology & medicine 2 33460768
2024 Papain expression in the Escherichia coli cytoplasm by T7-promoter engineering and co-expression with human protein disulfide isomerase (PDI) and thiol peroxidase (GPx7) genes. Applied and environmental microbiology 1 39589110
2024 Computational Mutagenesis of GPx7 and GPx8: Structural and Stability Insights into Rare Genetic and Somatic Missense Mutations and Their Implications for Cancer Development. Cancers 1 39796732