Affinage

GPSM3

G-protein-signaling modulator 3 · UniProt Q9Y4H4

Length
160 aa
Mass
17.9 kDa
Annotated
2026-06-10
96 papers in source corpus 10 papers cited in narrative 10 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

GPSM3 (G18/AGS4) is a regulator of heterotrimeric G-protein signaling that integrates multiple biochemically distinct activities to control chemokine-directed leukocyte responses and innate immune signaling (PMID:20097748, PMID:28062526). It engages Gαi through two functional modules: tandem GoLoco motifs that act as GDP dissociation inhibitors on Gαi·GDP, and a proline-rich N-terminal domain that binds activated Gα subunits and exhibits guanine nucleotide exchange activity toward Gαi1 (PMID:20097748). The integrity of the Gαi·GPSM3 interaction is required for recruitment of GPSM3 to the plasma membrane and for normal mitotic spindle dynamics (PMID:18984596). GPSM3 also binds nascent monomeric Gβ subunits through a leucine-rich region N-terminal to its first GoLoco motif, associating with the chaperones PhLP and CCT7 at the Golgi to stabilize Gβ prior to Gβγ dimer formation; both Gβ and Gαi·GDP binding are required for its inhibition of phospholipase-Cβ and for Akt activation (PMID:22167191). Phosphorylation of its N-terminal disordered region creates a 14-3-3 binding site that retains GPSM3 in the cytoplasm and protects it from proteasomal degradation (PMID:22843681). At the cellular level, GPSM3 is required for chemoattractant-directed chemotaxis and ERK activation in monocytes, neutrophils, dendritic cells, and T lymphocytes (PMID:28062526, PMID:23280397), directly suppresses the NLRP3 inflammasome by binding its C-terminal LRR domain to limit IL-1β production (PMID:25271165), and selectively partners with RGS5 to maintain GTPase-accelerating activity on Gαi1 (PMID:25842189). GPSM3 deficiency protects mice from inflammatory arthritis, and promoter variants that reduce GPSM3 expression impair chemokine-directed migration (PMID:23280397, PMID:26821282).

Mechanistic history

Synthesis pass · year-by-year structured walk · 9 steps
  1. 2008 High

    Established that the Gαi·GPSM3 interaction occurs through the canonical GoLoco-binding surface and is functionally consequential, linking the complex to membrane recruitment and mitotic spindle behavior.

    Evidence Rational mutagenesis of the Gαi helical domain (GoLoco-insensitive 'GLi' mutant) with binding, plasma-membrane recruitment, and live-cell spindle imaging in kidney epithelial cells

    PMID:18984596

    Open questions at the time
    • Spindle phenotype shown by ectopic Gαi expression in epithelial cells, not in physiological GPSM3-expressing hematopoietic cells
    • Did not resolve which GPSM3 module mediates the membrane recruitment
  2. 2010 High

    Resolved that GPSM3 carries two opposing G-protein regulatory activities — N-terminal GEF activity toward Gαi1 and GoLoco-mediated GDI activity — defining it as a bifunctional regulator rather than a simple GDI.

    Evidence In vitro [35S]GTPγS binding and AlF4- pull-downs with purified recombinant full-length, N-terminal (G18ΔC), and GoLoco-only (ΔNG18) domains

    PMID:20097748

    Open questions at the time
    • Activities measured in vitro with isolated domains; their integration on intact heterotrimers in cells not established
    • No effect on intrinsic GTPase activity, leaving the cellular consequence of the GEF activity unclear
  3. 2011 High

    Showed GPSM3 acts beyond Gα by binding free Gβ subunits during biogenesis, assigning it a chaperone-like role in G-protein assembly and linking both Gβ and Gαi binding to downstream PLCβ inhibition and Akt activation.

    Evidence Co-IP, BiFC, GST pulldown, domain mapping, PLCβ and Akt assays, and immunofluorescence colocalization in THP-1 cells

    PMID:22167191

    Open questions at the time
    • The order of binding events relative to PhLP/CCT7 chaperone handoff not fully resolved
    • Whether Gβ stabilization is rate-limiting for cellular Gβγ output not quantified
  4. 2012 High

    Defined a post-translational control layer in which phosphorylation-dependent 14-3-3 binding governs GPSM3 cytoplasmic retention and stability, coupling its abundance and localization to kinase signaling.

    Evidence Mass-spectrometry identification of 14-3-3 partners, phosphorylation-site mutagenesis, reciprocal Co-IP, subcellular fractionation, and immunofluorescence

    PMID:22843681

    Open questions at the time
    • The specific proline-directed kinase responsible for the phosphorylation not identified
    • Physiological stimuli that trigger this regulation in immune cells unknown
  5. 2012 High

    Connected GPSM3 to organism-level inflammatory disease, showing it is required in monocytes for chemokine-directed migration and survival and that its loss protects against arthritis.

    Evidence Gpsm3 knockout mice in the collagen antibody-induced arthritis model, transwell chemotaxis to CCL2/CX3CL1/chemerin, apoptosis assays, and flow cytometry

    PMID:23280397

    Open questions at the time
    • Did not distinguish which GPSM3 biochemical activity (GDI/GEF/Gβ chaperone) drives the migration defect
    • Mechanism linking GPSM3 loss to enhanced apoptosis not defined
  6. 2014 High

    Identified a G-protein-independent function: direct binding to the NLRP3 LRR domain establishes GPSM3 as a negative regulator of inflammasome activity and IL-1β output.

    Evidence Yeast two-hybrid, domain-mapped Co-IP, Gpsm3-null BMDM IL-1β/TNF-α ELISA, and in vivo Alum peritonitis

    PMID:25271165

    Open questions at the time
    • Whether GPSM3-NLRP3 binding affects inflammasome assembly, priming, or activation steps not mechanistically dissected
    • Relationship between this function and GPSM3's G-protein roles unresolved
  7. 2015 Medium

    Demonstrated selective coupling between GPSM3 and a specific RGS protein, showing GPSM3 enhances RGS5-mediated GTPase acceleration on Gαi1 under receptor-activated conditions.

    Evidence Co-IP selectivity screen across RGS proteins and solution/membrane-based GTP hydrolysis assays with a reconstituted M2 receptor system

    PMID:25842189

    Open questions at the time
    • Single laboratory with limited replication
    • Physiological consequence of the GPSM3-RGS5 module in vascular smooth muscle not tested in vivo
  8. 2016 Medium

    Linked human GPSM3 promoter variants to reduced transcript levels and showed that this expression reduction impairs chemokine-directed migration, providing a mechanistic basis for the disease-associated allele.

    Evidence Human cohort genotyping, qRT-PCR, promoter-reporter assays, and siRNA knockdown chemotaxis (toward CCL2 in THP-1; toward LTB4/CXCL8 vs fMLP in NB4-derived neutrophils)

    PMID:26821282 PMID:27307211

    Open questions at the time
    • Chemoattractant selectivity (LTB4/CXCL8 dependent, fMLP independent) not explained at the receptor-signaling level
    • Knockdown in cell-line models rather than primary human leukocytes
  9. 2017 High

    Generalized the chemotaxis requirement across leukocyte lineages and established that the GPSM3-Gαi interaction is regulated agonist-dependently at the receptor, with organismal consequences for blood cell counts and inflammation.

    Evidence Gpsm3 knockout mice, complete blood counts, chemotaxis and ERK assays across dendritic cells/T cells/neutrophils, and an in vivo peritonitis migration model

    PMID:28062526

    Open questions at the time
    • The molecular basis of agonist-dependent regulation of the AGS4-Gαi interaction not resolved
    • Cause of the neutropenia/leukocytosis phenotype not mechanistically traced

Open questions

Synthesis pass · forward-looking unresolved questions
  • How GPSM3's distinct biochemical modules (GoLoco-GDI, N-terminal GEF, Gβ chaperone, NLRP3 binding, RGS5 enhancement) are coordinated within a single cell to produce chemoattractant-selective signaling and inflammasome control remains unresolved.
  • No unified model integrating the G-protein and NLRP3 functions
  • The kinase and stimuli controlling 14-3-3-dependent localization in immune cells unidentified
  • No structural model of GPSM3 bound to its various partners

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 3 GO:0044183 protein folding chaperone 1 GO:0060089 molecular transducer activity 1 GO:0140096 catalytic activity, acting on a protein 1
Localization
GO:0005829 cytosol 2 GO:0005886 plasma membrane 2 GO:0005794 Golgi apparatus 1
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-168256 Immune System 3 R-HSA-392499 Metabolism of proteins 2
Partners
CCT7GNAI1GNB1NLRP3PDCLRGS5YWHAB

Evidence

Reading pass · 10 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2008 A point mutation (asparagine-to-isoleucine) in the αD-αE loop of the Gαi helical domain selectively abolishes GoLoco motif binding. This GoLoco-insensitivity ('GLi') mutation prevented Gαi1 association with all human GoLoco motif proteins including GPSM3, and abrogated GPSM3 recruitment to the plasma membrane by Gαi subunits. Ectopic expression of wild-type Gαi (but not the GLi mutant) caused exaggerated mitotic spindle rocking in kidney epithelial cells, directly implicating the Gαi·GPSM3 complex in mitotic spindle dynamics. Rational mutagenesis of Gαi, biochemical binding assays, cell-based plasma membrane recruitment assays, live-cell imaging of mitotic spindle behavior The Journal of biological chemistry High 18984596
2011 GPSM3 interacts with Gβ1–Gβ4 subunits independently of Gγ or Gα·GDP subunits. The Gβ-interaction site was mapped to a leucine-rich region N-terminal to GPSM3's first GoLoco motif. The GPSM3–Gβ complex forms at and transits through the Golgi apparatus and also exists as a soluble cytoplasmic complex; endogenously, GPSM3 and Gβ co-localize in THP-1 cells at the plasma membrane and in a juxtanuclear compartment. GPSM3 increases Gβ stability prior to Gβγ dimer formation and associates with chaperones PhLP and CCT7 during Gβ biosynthesis. Both Gβ and Gαi·GDP binding by GPSM3 are required for its inhibition of phospholipase-Cβ activation. GPSM3 is also required for Akt activation in THP-1 cells, a known Gβγ-dependent pathway. Co-immunoprecipitation, bimolecular fluorescence complementation (BiFC), GST pulldown, domain-deletion mapping, phospholipase-Cβ activity assay, Akt phosphorylation assay, immunofluorescence colocalization The Journal of biological chemistry High 22167191
2010 The proline-rich N-terminal domain of GPSM3 (G18) functions as a novel G-protein regulatory region distinct from its GoLoco motifs. In the presence of fluoroaluminate (AlF4−), full-length GPSM3 and its isolated N-terminal domain (G18ΔC) bound strongly to activated Gαi and Gαo, whereas the isolated GoLoco region (ΔNG18) did not. The N-terminal region exhibited guanine nucleotide exchange factor (GEF) activity toward Gαi1 (promoting [35S]GTPγS binding), while the GoLoco region acted as a GDP dissociation inhibitor. On Gαo, the N-terminal region inhibited [35S]GTPγS binding. Neither full-length GPSM3 nor any mutant affected the GTPase activity of Gαi1 or Gαo. In vitro [35S]GTPγS binding assays with purified recombinant proteins, AlF4− activated Gα pull-downs, domain deletion mutagenesis The Journal of biological chemistry High 20097748
2012 GPSM3 subcellular localization is regulated by phosphorylation-dependent interaction with 14-3-3 proteins. A proline-directed serine/threonine kinase phosphorylates GPSM3, creating a mode II consensus 14-3-3 binding site in its N-terminal disordered region. 14-3-3 binding stabilizes GPSM3 from proteasomal degradation. The GPSM3–14-3-3 complex is exclusively cytoplasmic, and both proteins mutually control their exclusion from the nucleus. In silico binding site prediction, proteomics/mass spectrometry identification of 14-3-3 partners, co-immunoprecipitation, phosphorylation-site mutagenesis, subcellular fractionation, immunofluorescence localization The Journal of biological chemistry High 22843681
2012 GPSM3 deficiency in mice protects against collagen antibody-induced arthritis (CAIA). GPSM3 is expressed at highest levels in mature monocytes. GPSM3-deficient myeloid cells showed reduced migration ex vivo toward CCL2, CX3CL1, and chemerin, and enhanced apoptosis in vitro. Expression of monocyte-representative pro-inflammatory chemokine receptors and cytokines in paws of Gpsm3−/− mice was decreased. Gpsm3 knockout mice, CAIA disease model (clinical scoring, histopathology), flow cytometry, transwell chemotaxis assay, apoptosis assay, immunoblotting Molecular immunology High 23280397
2014 GPSM3 directly interacts with the C-terminal leucine-rich repeat (LRR) domain of NLRP3 and acts as a negative regulator of NLRP3 inflammasome activity. Bone marrow-derived macrophages from Gpsm3−/− mice showed increased NLRP3-dependent IL-1β (but not TNF-α) secretion. Gpsm3-null mice exhibited enhanced serum and peritoneal IL-1β production following Alum-induced peritonitis. Yeast two-hybrid screen (initial identification), co-immunoprecipitation (GPSM3–NLRP3 LRR domain), Gpsm3 knockout macrophages, ELISA for IL-1β/TNF-α, in vivo Alum peritonitis model The Journal of biological chemistry High 25271165
2015 GPSM3 selectively binds RGS5 (but not other tested RGS proteins) and enhances RGS5-accelerated GTP hydrolysis by Gαi1 in solution-based assays. In membrane-based assays with M2 muscarinic receptor-activated Gαi1, GPSM3 decreased GTP hydrolysis in the presence of RGS4 but not RGS5, indicating that the GPSM3–RGS5 complex maintains enhanced GTPase-accelerating activity under physiological receptor-activation conditions. Both GPSM3 and RGS5 are expressed in primary rat aortic smooth muscle cells. Co-immunoprecipitation (selectivity screen across RGS proteins), in vitro GTP hydrolysis assay (solution and membrane-based with reconstituted M2 receptor system), immunoblotting of primary cells Molecular and cellular biochemistry Medium 25842189
2016 GPSM3 is induced during retinoic acid-driven differentiation of NB4 cells into a neutrophil model. Reducing GPSM3 expression (mimicking the arthritis-protective SNP rs204989 effect) specifically disrupted migration toward leukotriene B4 (LTB4) and interleukin-8 (CXCL8) but not toward formylated peptides (fMLP), indicating chemoattractant-selective signaling dependence on GPSM3 levels in neutrophil-like cells. Retinoic acid differentiation of NB4 cells, siRNA knockdown of GPSM3, transwell chemotaxis assay toward LTB4, CXCL8, and fMLP Genes and immunity Medium 27307211
2016 GPSM3 SNPs rs204989 and rs204991 (upstream of the transcription start site) reduce GPSM3 transcript abundance in individuals homozygous for the minor allele. In vitro promoter activity studies indicate rs204989 is the primary functional variant. Knockdown of GPSM3 in THP-1 monocytes disrupts migration toward MCP-1 (CCL2). Human cohort genotyping, GPSM3 transcript quantification by qRT-PCR, in vitro promoter-reporter assays, siRNA knockdown in THP-1 cells, chemotaxis assay Genes and immunity Medium 26821282
2017 AGS4/GPSM3-null mice exhibit mild but significant neutropenia and leukocytosis. Dendritic cells, T lymphocytes, and neutrophils from AGS4/GPSM3-null mice show significant defects in chemoattractant-directed chemotaxis and ERK activation. In a peritonitis model, AGS4/GPSM3-null neutrophils had dramatically reduced ability to migrate to sites of inflammation. The AGS4–Gαi interaction is regulated in an agonist-dependent, receptor-proximal manner by chemokine receptors. Gpsm3 knockout mice, complete blood count, in vitro chemotaxis assay (multiple leukocyte types), ERK phosphorylation assay, in vivo peritonitis model The Journal of pharmacology and experimental therapeutics High 28062526

Source papers

Stage 0 corpus · 96 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
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2002 Leptin mediates the parathyroid hormone-related protein paracrine stimulation of fetal lung maturation. American journal of physiology. Lung cellular and molecular physiology 118 11839533
2010 Differential expression and activity of the porcine type I interferon family. Physiological genomics 86 20406849
2006 Mechanism-based pharmacokinetic-pharmacodynamic modeling of the antinociceptive effect of buprenorphine in healthy volunteers. Anesthesiology 65 16732095
2017 Autophagy Is an Innate Mechanism Associated with Leprosy Polarization. PLoS pathogens 54 28056107
1982 Demonstration at the single-cell level of the existence of distinct clusters of epitopes in two predefined human Ia molecular subsets. European journal of immunology 52 6176457
2014 Flower-like ZnO nanostructure based electrochemical DNA biosensor for bacterial meningitis detection. Biosensors & bioelectronics 51 24727606
2012 Pharmacokinetic and pharmacodynamic modelling of intravenous, intramuscular and subcutaneous buprenorphine in conscious cats. Veterinary anaesthesia and analgesia 51 22998333
2017 Electrochemical detection of cardiac biomarker myoglobin using polyphenol as imprinted polymer receptor. Analytica chimica acta 49 28693728
2021 Ti3C2-MXene decorated with nanostructured silver as a dual-energy acceptor for the fluorometric neuron specific enolase detection. Biosensors & bioelectronics 48 34560349
2005 Soluble forms of EphrinB2 and EphB4 reduce retinal neovascularization in a model of proliferative retinopathy. Investigative ophthalmology & visual science 46 15914639
2001 Physiological hyperinsulinemia impairs insulin-stimulated glycogen synthase activity and glycogen synthesis. American journal of physiology. Endocrinology and metabolism 46 11287353
2018 CRISPR/Cas9‒Mediated Tspo Gene Mutations Lead to Reduced Mitochondrial Membrane Potential and Steroid Formation in MA-10 Mouse Tumor Leydig Cells. Endocrinology 43 29300865
2023 Self-Immolative Photosensitizers for Self-Reported Cancer Phototheranostics. Journal of the American Chemical Society 42 37216494
2012 Surfactant aided biodegradation of NAPLs by Burkholderia multivorans: comparison between Triton X-100 and rhamnolipid JBR-515. Colloids and surfaces. B, Biointerfaces 41 23104033
2021 IL-17A Damages the Blood-Retinal Barrier through Activating the Janus Kinase 1 Pathway. Biomedicines 40 34356895
2008 A point mutation to Galphai selectively blocks GoLoco motif binding: direct evidence for Galpha.GoLoco complexes in mitotic spindle dynamics. The Journal of biological chemistry 40 18984596
2017 Protective, repairing and fibrinolytic effects of rivaroxaban on vascular endothelium. British journal of clinical pharmacology 36 28940408
1995 Steroidogenic activity, insulin-like growth factor-I production, and proliferation of granulosa and theca cells obtained from dominant preovulatory and nonovulatory follicles during the bovine estrous cycle: effects of low-density and high-density lipoproteins. Biology of reproduction 33 8562681
2022 Mitochondrial dynamics, Leydig cell function, and age-related testosterone deficiency. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 32 36349989
2019 Carboxymethyl chitosan-based nanogels via acid-labile ortho ester linkages mediated enhanced drug delivery. International journal of biological macromolecules 27 30771386
2017 Haemocytes collected from experimentally infected Pacific oysters, Crassostrea gigas: Detection of ostreid herpesvirus 1 DNA, RNA, and proteins in relation with inhibition of apoptosis. PloS one 27 28542284
2005 Phenotypic and genotypic characteristics of Streptococcus porcinus isolated from human sources. Journal of clinical microbiology 27 16145112
2014 G Protein signaling modulator-3 inhibits the inflammasome activity of NLRP3. The Journal of biological chemistry 26 25271165
2011 Characterization and quantitation of a novel β-lactamase gene found in a wastewater treatment facility and the surrounding coastal ecosystem. Applied and environmental microbiology 26 21965412
2002 VEGF(121), VEGF(165) overexpression enhances tumorigenicity in U251 MG but not in NG-1 glioma cells. Cancer research 26 11912165
1990 Inhibition of epidermal growth factor receptor activity by retinoic acid in glioma cells. Journal of cellular biochemistry 25 2307713
2012 G-protein signaling modulator-3, a gene linked to autoimmune diseases, regulates monocyte function and its deficiency protects from inflammatory arthritis. Molecular immunology 24 23280397
1998 Cross-talking among Drosophila nuclear receptors at the promiscuous response element of the ng-1 and ng-2 intermolt genes. Journal of molecular biology 23 9466931
2021 Carbon cloth-based immunosensor for detection of 25-hydroxy vitamin D3. Mikrochimica acta 22 33792779
2022 Optimization of enzymatic fragmentation is crucial to maximize genome coverage: a comparison of library preparation methods for Illumina sequencing. BMC genomics 21 35105301
2006 Interleukin-1beta signals through a c-Jun N-terminal kinase-dependent inducible nitric oxide synthase and nitric oxide production pathway in Sertoli epithelial cells. Endocrinology 20 16887914
2021 Antimicrobial-specific response from resistance gene carriers studied in a natural, highly diverse microbiome. Microbiome 19 33504360
2014 G protein signaling modulator-3: a leukocyte regulator of inflammation in health and disease. American journal of clinical and experimental immunology 19 25143870
2023 Enhanced Electrochemical Biosensing of the Sp17 Cancer Biomarker in Serum Samples via Engineered Two-Dimensional MoS2 Nanosheets on the Reduced Graphene Oxide Interface. ACS applied bio materials 18 37715717
2012 Systemic nocardiosis in a Mediterranean population of cultured meagre, Argyrosomus regius Asso (Perciformes: Sciaenidae). Journal of fish diseases 18 23094711
2023 Three point mutations in AaCYP51 combined with induced overexpression of AaCYP51 conferred low-level resistance to mefentrifluconazole in Alternaria alternata. Pesticide biochemistry and physiology 17 38072534
2018 Presence of aggregates of smooth endoplasmic reticulum in MII oocytes affects oocyte competence: molecular-based evidence. Molecular human reproduction 17 29635518
2015 Gelsolin, NF-κB, and p53 expression in clear cell renal cell carcinoma: Impact on outcome. Pathology, research and practice 17 25908108
2023 A disposable immunosensor for the detection of salivary MMP-8 as biomarker of periodontitis. Bioelectrochemistry (Amsterdam, Netherlands) 16 37976772
2020 Pre-operative cellular dissociation grading in biopsies is highly predictive of post-operative tumour stage and patient outcome in head and neck squamous cell carcinoma. British journal of cancer 16 31937923
2019 Fabrication of size-controllable human mesenchymal stromal cell spheroids from micro-scaled cell sheets. Biofabrication 16 31096204
2021 Fabrication of GQD-Electrodeposited Screen-Printed Carbon Electrodes for the Detection of the CRP Biomarker. ACS omega 15 34901602
2015 Surfactant-free preparation of highly stable zwitterionic poly(amido amine) nanogels with minimal cytotoxicity. Acta biomaterialia 15 26518103
1986 Prostacyclin and steroidogenesis in goat ovarian cell types in vitro. Prostaglandins 15 3086937
2022 Trunk injection delivery of dsRNA for RNAi-based pest control in apple trees. Pest management science 14 35578562
2011 G-protein signaling modulator-3 regulates heterotrimeric G-protein dynamics through dual association with Gβ and Gαi protein subunits. The Journal of biological chemistry 14 22167191
2010 Inhibition of central angiotensin II-induced pressor responses by hydrogen peroxide. Neuroscience 14 20832454
1997 Insulin-like growth factors (IGF-I and IGF-II) and IGF-binding protein-3 production by fibroblasts of patients with Turner's syndrome in culture. The Journal of clinical endocrinology and metabolism 14 9100570
1997 Protein kinase C modulator effects on parathyroid hormone-induced intracellular calcium and morphologic changes in UMR 106-H5 osteoblastic cells. Journal of cellular biochemistry 14 9136085
2022 New phthalonitrile/metal phthalocyanine-gold nanoparticle conjugates for biological applications. Dalton transactions (Cambridge, England : 2003) 13 35229854
2017 N-glycosylation of serum proteins for the assessment of patients with IgD multiple myeloma. BMC cancer 13 29268706
2009 Drosophila suppressor of sable protein [Su(s)] promotes degradation of aberrant and transposon-derived RNAs. Molecular and cellular biology 13 19687295
2012 A simple, cost-effective and flexible method for processing of snap-frozen tissue to prepare large amounts of intact RNA using laser microdissection. Biochimie 12 22796379
2023 Plant Virus Sensor for the Rapid Detection of Bean Pod Mottle Virus Using Virus-Specific Nanocavities. ACS sensors 11 37738225
2014 Involvement of p38 mitogen-activated protein kinase in acquired gemcitabine-resistant human urothelial carcinoma sublines. The Kaohsiung journal of medical sciences 11 24924837
2010 The proline-rich N-terminal domain of G18 exhibits a novel G protein regulatory function. The Journal of biological chemistry 11 20097748
1987 Kinetics of carcinoembryonic antigen and carbohydrate antigen 19-9 production in a human pancreatic cancer cell line (SUIT-2). Gastroenterologia Japonica 11 2445616
2023 Clinical significance of tumor cell seeding associated with needle biopsy in patients with breast cancer. Asian journal of surgery 10 36732183
2021 Transcriptional Changes in Regulatory T Cells From Patients With Autoimmune Polyendocrine Syndrome Type 1 Suggest Functional Impairment of Lipid Metabolism and Gut Homing. Frontiers in immunology 10 34526996
2014 Synthesis of thermoresponsive polymers for drug delivery. Methods in molecular biology (Clifton, N.J.) 10 24567132
2013 The value of progesterone receptor expression in predicting the Recurrence Score for hormone-receptor positive invasive breast cancer patients. Breast cancer (Tokyo, Japan) 9 24081457
2009 Clinical significance of vascular endothelial growth factor expression in patients with carcinoma of the mouth floor and tongue. Vojnosanitetski pregled 9 19583141
2006 Neurogenesin-1 differentially inhibits the osteoblastic differentiation by bone morphogenetic proteins in C2C12 cells. Biochemical and biophysical research communications 9 16630536
2011 AtNG1 encodes a protein that is required for seed germination. Plant science : an international journal of experimental plant biology 8 21889052
2009 Chemical and electroporated transformation of Edwardsiella ictaluri using three different plasmids. FEMS microbiology letters 8 19594620
2012 Regulation of the subcellular localization of the G-protein subunit regulator GPSM3 through direct association with 14-3-3 protein. The Journal of biological chemistry 7 22843681
2025 Genetic Commonalities Between Metabolic Syndrome and Rheumatic Diseases Through Disease Interactome Modules. Journal of cellular and molecular medicine 6 39789419
2024 Comammox Nitrospira are the dominant ammonia oxidizers in the Yangtze estuarine biofilms. Water research 6 39689421
2023 Nanoengineered phosphorus doped graphitic carbon nitride based ultrasensitive biosensing platform for Swine flu detection. Colloids and surfaces. B, Biointerfaces 6 37597493
2023 The role and expression of pro/antiangiogenic factors and microRNAs in gestational hypertension and pre-eclampsia. European journal of obstetrics, gynecology, and reproductive biology 6 37716201
2017 Regulation of Chemokine Signal Integration by Activator of G-Protein Signaling 4 (AGS4). The Journal of pharmacology and experimental therapeutics 6 28062526
2016 Genetic variations in GPSM3 associated with protection from rheumatoid arthritis affect its transcript abundance. Genes and immunity 6 26821282
2024 Fish-hunting cone snail disrupts prey's glucose homeostasis with weaponized mimetics of somatostatin and insulin. Nature communications 5 39164229
2021 Sodium palatability in male spontaneously hypertensive rats. Hormones and behavior 5 33647281
2021 Characterization and functional properties of gelatin from goat bone through alcalase and neutrase enzymatic extraction. Veterinary world 5 34840460
2023 Optimized Carbohydrate-Based Nanogel Formulation to Sensitize Hypoxic Tumors. Molecular pharmaceutics 4 37148327
2023 A semi-automated microscopic image analysis method for scoring Ki-67 nuclear immunostaining. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 4 37970922
2016 Reduction of GPSM3 expression akin to the arthritis-protective SNP rs204989 differentially affects migration in a neutrophil model. Genes and immunity 4 27307211
2017 The value of Nottingham grade in breast cancer re-visited in the Sri Lankan setting. The Malaysian journal of pathology 3 28866695
2015 Regulation of RGS5 GAP activity by GPSM3. Molecular and cellular biochemistry 3 25842189
2024 A Simple and Efficient One-Step Synthesis System for Flexible Production of Circular RNA in E. coli. Biomolecules 2 39595592
2022 An observational study of pro- and anti-angiogenic factors in hypertensive disorders of pregnancy in women of African ancestry. Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology 2 35866241
2020 Effects of preterm birth induced with or without exogenous glucocorticoids on the ovine glucose-insulin axis. Journal of developmental origins of health and disease 2 31937391
2019 Histamine H3 receptor activation reduces the impairment in prepulse inhibition (PPI) of the acoustic startle response and Akt phosphorylation induced by MK-801 (dizocilpine), antagonist at N-Methyl-d-Aspartate (NMDA) receptors. Progress in neuro-psychopharmacology & biological psychiatry 2 31108178
2001 Functional analysis of regulatory elements controlling the expression of the ecdysone-regulated Drosophila ng-1 gene. Mechanisms of development 2 11118881
2025 [Establishment and Evaluation of a Nucleic Acid Amplification Test for Spectinomycin-Resistant Neisseria gonorrhoeae]. Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 1 40109447
2025 Deep learning helps fight against antibiotic resistance. Cell host & microbe 1 41067213
2025 Single-cell RNA-seq combined with bulk RNA-seq analysis identifies necroptosis-related genes as therapeutic targets for periodontitis. BMC medical genomics 1 41088263
2023 Resorc[4]arene Modifiers for Supramolecular Site-Directed Immobilization of Antibodies on Multi-Walled Carbon Nanotubes. Chembiochem : a European journal of chemical biology 1 36867729
2022 Stability improvement of polyaniline nanocomposite immunosensor for early detection of insulin receptor antibody as biomarker of type 2 diabetes. Mikrochimica acta 1 36322195
2025 [Feasibility of polyetheretherketone loaded with bone morphogenetic protein 2 for orbital fracture repair]. [Zhonghua yan ke za zhi] Chinese journal of ophthalmology 0 41218902
2025 Click Detect: A Rapid and Sensitive Assay for Shiga Toxin 2 Detection. Biosensors 0 41440294
2022 Proliferative epithelial changes in tumour adjacent tissue in Sri Lankan women with breast carcinoma: do morphological changes support molecular models of breast carcinogenesis? Diagnostic pathology 0 36581929

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