| 2023 |
FOXO3A directly binds to the promoter of GNG7 and preserves its expression; GNG7 protein in turn interacts with mTOR and restricts phosphorylated (activated) mTOR. Loss of FOXO3A reduces GNG7 expression, thereby releasing mTOR suppression and causing adaptive resistance in AML cells. |
Chromatin immunoprecipitation (FOXO3A binding to GNG7 promoter), co-immunoprecipitation (GNG7–mTOR interaction), loss-of-function/rescue experiments, mouse AML model, patient-derived xenograft model |
Genes & diseases |
High |
37588187
|
| 2024 |
GNG7 is a transmembrane binding partner of RSPO3; Co-IP and LC-MS/MS confirmed direct physical interaction. RSPO3 increases phosphorylation of Akt and GSK-3β and stabilises β-catenin to promote gastric cancer stem-cell properties, and this effect is blocked by GNG7 knockdown, placing GNG7 upstream of the Akt/GSK-3β/β-catenin axis in this pathway. |
Co-immunoprecipitation + LC-MS/MS (RSPO3–GNG7 interaction), siRNA knockdown of GNG7 with rescue of β-catenin/Akt/GSK-3β phosphorylation, spheroid-formation and in vivo peritoneal-seeding assays |
Cancer medicine |
Medium |
38581123
|
| 2026 |
H3K18 lactylation suppresses GNG7 transcription: ChIP-qPCR showed elevated H3K18la enrichment at the GNG7 promoter in bladder cancer cells, correlating with reduced GNG7 expression. Overexpression of GNG7 inhibited PI3K-AKT pathway activation and reduced glycolysis and tumor growth. |
ChIP-qPCR (H3K18la at GNG7 promoter), GNG7 overexpression with Western blot for PI3K-AKT, glycolysis assays (glucose uptake, lactate production, ECAR), xenograft mouse model |
World journal of surgical oncology |
Medium |
42163331
|
| 2021 |
GNG7 overexpression in lung adenocarcinoma cells inhibits activation of Hedgehog signaling and suppresses proliferation and invasion in vitro and tumor formation in vivo. miR-19b-3p directly targets GNG7 (verified by reporter assay), and GNG7 re-introduction rescues miR-19b-3p-driven aggressive phenotypes. |
GNG7 gain-of-function (overexpression), Hedgehog pathway reporter/western blot, dual-luciferase miRNA target validation, xenograft assay |
Bioengineered |
Medium |
34635014
|
| 2019 |
GNG7 silencing in placental cytotrophoblasts activates the mTOR signaling pathway (increased phosphorylation of mTOR, p70S6K, 4E-BP1), enhances cell proliferation and differentiation, and inhibits apoptosis. Conversely, mTOR inhibition (rapamycin) blocks the pro-proliferative effects of GNG7 knockdown, establishing GNG7 as a negative regulator of mTOR in trophoblasts. |
siRNA knockdown of GNG7, mTOR pathway activator (HIV-1 Tat) and inhibitor (rapamycin) treatments, proliferation/apoptosis assays, Western blot for mTOR/p70S6K/4E-BP1, ELISA for sFlt-1/sEng |
International journal of molecular medicine |
Medium |
30864685
|
| 2025 |
CAF-derived extracellular vesicles transfer miR-6765-3p to colorectal cancer cells; miR-6765-3p directly targets the 3'-UTR of GNG7 (dual-luciferase assay), reducing GNG7 expression, which releases mTOR pathway inhibition and promotes aerobic glycolysis. GNG7 overexpression rescues mTOR phosphorylation suppression and reduces CRC progression. |
Dual-luciferase 3'-UTR reporter assay, GNG7 overexpression rescue, mTOR pathway Western blot, glycolysis assays, cellular and xenograft experiments |
Cellular signalling |
Medium |
41352746
|
| 2022 |
LINC01526 interacts with TARBP2 and this complex decreases GNG7 mRNA stability, leading to GNG7 mRNA decay and enhanced gastric cancer proliferation and migration. Rescue experiments showed that GNG7 downregulation partially reversed the anti-proliferative effect of LINC01526 or TARBP2 silencing. |
RNA immunoprecipitation (LINC01526–TARBP2 interaction), mRNA stability assay, siRNA knockdown, functional rescue experiments in vitro and xenograft |
Cancers |
Medium |
36230863
|
| 2021 |
In sebocytes, miR-146a inversely regulates GNG7 expression (miR-146a inhibitor induces GNG7; miR-146a mimic suppresses GNG7). GNG7 inhibition in SZ95 sebocytes increases lipid content and decreases proliferation, indicating GNG7 restrains lipid production and promotes proliferation downstream of TLR1/2 and TLR4 signaling. |
miR-146a inhibitor/mimic treatment with gene expression profiling, GNG7 siRNA knockdown with lipid content and proliferation assays, in situ hybridization in acne tissue |
Scientific reports |
Medium |
34728702
|
| 2008 |
GNG7 overexpression in oesophageal cancer cells reduced tumour invasion in vitro and in vivo. GNG7 suppression in patient tumours was associated with promoter hypermethylation or loss of heterozygosity, establishing epigenetic silencing as a mechanism of GNG7 downregulation. |
GNG7 transfection/overexpression with invasion assays, LOH analysis, bisulfite sequencing/methylation-specific PCR for promoter hypermethylation |
British journal of cancer |
Medium |
18219292
|