| 2021 |
SNORD50A/B small nucleolar RNAs enhance the interaction between E3 ubiquitin ligase TRIM21 and GMPS by forming a complex among them, promoting GMPS ubiquitination and cytoplasmic sequestration; loss of SNORD50A/B releases GMPS to translocate into the nucleus, where GMPS recruits USP7 and forms a complex with p53, decreasing p53 ubiquitination and stabilizing p53 protein. |
Co-immunoprecipitation, western blot, immunofluorescence, functional overexpression/knockout studies in breast cancer cells |
Cell death and differentiation |
High |
33742136
|
| 2020 |
TRIM21 acts as an E3 ubiquitin ligase that mediates GMPS ubiquitination and degradation, thereby reducing nuclear GMPS availability and suppressing TP53 expression; SERPINB5 serves as an adaptor protein that prevents GMPS from entering the nucleus and recruits TRIM21 for GMPS ubiquitination. |
Co-immunoprecipitation, western blot, mass spectrometry identification of GMPS as TRIM21 target, immunofluorescence, overexpression/knockout cell lines, in vivo xenograft models |
Journal of biomedical science |
High |
32005234
|
| 2024 |
GMPS promotes PD-L1 expression in hepatocellular carcinoma by enhancing the interaction between PD-L1 and the catalytic subunit STT3A of oligosaccharyltransferase (OST), acting as an additional module connecting the Sec61 channel complex and STT3A, thereby facilitating translocation and N-glycosylation of PD-L1 and reducing its ubiquitination-mediated degradation. |
Proteomic and scRNA-seq analyses, co-immunoprecipitation, western blot, pharmacological inhibition with angustmycin A, in vivo tumor models |
Cell death and differentiation |
Medium |
39690246
|
| 2021 |
GMPS functions in the de novo purine biosynthesis pathway utilizing a glutamine amide to synthesize guanosine monophosphate; pharmacological inhibition or knockdown of GMPS significantly decreases prostate cancer cell growth and alters glutamine metabolism, as demonstrated by [15N-(amide)]glutamine and [U-13C5]glutamine metabolic tracing. |
siRNA knockdown, pharmacological inhibition, isotope metabolic tracing ([15N]-glutamine, [U-13C5]-glutamine), PC-3 xenograft mouse model |
The Journal of pathology |
Medium |
33768538
|
| 2000 |
GMPS (Guanosine 5'-Monophosphate Synthetase) gene on chromosome 3q24 is fused in-frame with MLL in a t(3;11)(q25;q23) translocation in treatment-related acute myeloid leukemia, producing a chimeric MLL-GMPS fusion transcript that is alternatively spliced. |
cDNA panhandle PCR, reverse transcription, Southern blot analysis |
Blood |
Medium |
11110714
|
| 2013 |
The GATase subunit of archaeal (Methanocaldococcus jannaschii) GMPS has a solution structure with a seven-stranded mixed β-sheet fenced by five α-helices; NMR chemical shift perturbation mapping identified a common surface on GATase responsible for both Mg2+ binding and interaction with the ATPPase subunit, with GATase-Mg2+ having Kd ~1 mM and GATase-ATPPase interaction falling in intermediate chemical exchange. |
Solution NMR structure determination, 15N backbone relaxation, NMR chemical shift perturbation mapping, gel filtration chromatography |
Biochemistry |
Medium |
23724776
|
| 2025 |
GMPS promotes lung cancer cell migration through the SERPINB2-uPA axis, with DNMT1 serving as an intermediate factor by which GMPS regulates SERPINB2 expression; knockdown of GMPS inhibits tumor progression in NSCLC. |
Lentiviral overexpression and knockout cell lines, RNA sequencing, western blot, animal experiments |
Cellular oncology (Dordrecht, Netherlands) |
Medium |
40498285
|
| 2025 |
Extracellular matrix (ECM) bio-scaffolds enhance guanylate production in pancreatic ductal adenocarcinoma cells via upregulation of GMPS (and IMPDH) expression, with increased guanylates alleviating oxaliplatin-induced DNA damage; GMPS expression correlates with matrisomal and DNA repair gene expression in PDAC patient samples. |
In vitro ECM bio-scaffolds, multi-omics integration, metabolic tracing, gene expression correlation in patient samples |
bioRxivpreprint |
Low |
|